AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180004620.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 13 sgRNAs with CRISPR-Local

Find 15 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACTCCTTTCTTGCCTCTTT+TGG 0.160489 1:-80414322 None:intergenic
TCTGGCACTGTTAATGTTGA+TGG 0.353049 1:+80414273 MsG0180004620.01.T01:CDS
TTCATCCATGTTCAATGGAT+TGG 0.389835 1:+80414147 MsG0180004620.01.T01:CDS
TTCTCCAAAAGAGGCAAGAA+AGG 0.392141 1:+80414318 MsG0180004620.01.T01:CDS
AAAGGAAGCACTAAATATAG+TGG 0.436790 1:+80414190 MsG0180004620.01.T01:CDS
CCCACACTAAGCAACAATCT+TGG 0.441948 1:-80414347 None:intergenic
CACTCCCTTATCAATAGCTC+TGG 0.468896 1:+80414255 MsG0180004620.01.T01:CDS
CAGTGCCAGAGCTATTGATA+AGG 0.477915 1:-80414260 None:intergenic
GAGAGAAGCTGCTGAAGCAT+TGG 0.524056 1:+80414213 MsG0180004620.01.T01:CDS
AGTGCCAGAGCTATTGATAA+GGG 0.543600 1:-80414259 None:intergenic
CTTTGCCAATCCATTGAACA+TGG 0.552225 1:-80414152 None:intergenic
AAGTCATTGAAAATGAAGAA+AGG 0.566697 1:+80414172 MsG0180004620.01.T01:CDS
AAGGAAGCACTAAATATAGT+GGG 0.587134 1:+80414191 MsG0180004620.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AAGTCATTGAAAATGAAGAA+AGG + Chr1:80414172-80414191 MsG0180004620.01.T01:CDS 25.0%
! CATTTTTCATCCATGTTCAA+TGG + Chr1:80414142-80414161 MsG0180004620.01.T01:CDS 30.0%
!! AAAGGAAGCACTAAATATAG+TGG + Chr1:80414190-80414209 MsG0180004620.01.T01:CDS 30.0%
!! AAGGAAGCACTAAATATAGT+GGG + Chr1:80414191-80414210 MsG0180004620.01.T01:CDS 30.0%
TTCATCCATGTTCAATGGAT+TGG + Chr1:80414147-80414166 MsG0180004620.01.T01:CDS 35.0%
AGTGCCAGAGCTATTGATAA+GGG - Chr1:80414262-80414281 None:intergenic 40.0%
CTTTGCCAATCCATTGAACA+TGG - Chr1:80414155-80414174 None:intergenic 40.0%
TCTGGCACTGTTAATGTTGA+TGG + Chr1:80414273-80414292 MsG0180004620.01.T01:CDS 40.0%
TTCTCCAAAAGAGGCAAGAA+AGG + Chr1:80414318-80414337 MsG0180004620.01.T01:CDS 40.0%
! AACTCCTTTCTTGCCTCTTT+TGG - Chr1:80414325-80414344 None:intergenic 40.0%
CACTCCCTTATCAATAGCTC+TGG + Chr1:80414255-80414274 MsG0180004620.01.T01:CDS 45.0%
CAGTGCCAGAGCTATTGATA+AGG - Chr1:80414263-80414282 None:intergenic 45.0%
! GCCTCAGTTTTCTCCAAAAG+AGG + Chr1:80414309-80414328 MsG0180004620.01.T01:CDS 45.0%
!! GCCTCTTTTGGAGAAAACTG+AGG - Chr1:80414313-80414332 None:intergenic 45.0%
GAGAGAAGCTGCTGAAGCAT+TGG + Chr1:80414213-80414232 MsG0180004620.01.T01:CDS 50.0%
Chromosome Type Strat End Strand Name
Chr1 gene 80414136 80414363 80414136 ID=MsG0180004620.01;Name=MsG0180004620.01
Chr1 mRNA 80414136 80414363 80414136 ID=MsG0180004620.01.T01;Parent=MsG0180004620.01;Name=MsG0180004620.01.T01;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|1|1|0|75
Chr1 exon 80414136 80414363 80414136 ID=MsG0180004620.01.T01:exon:13324;Parent=MsG0180004620.01.T01
Chr1 CDS 80414136 80414363 80414136 ID=MsG0180004620.01.T01:cds;Parent=MsG0180004620.01.T01
Gene Sequence

>MsG0180004620.01.T01

ATGGGTCATTTTTCATCCATGTTCAATGGATTGGCAAAGTCATTGAAAATGAAGAAAGGAAGCACTAAATATAGTGGGAGAGAAGCTGCTGAAGCATTGGCAAAAGAAGCAAAAGAAATCACTCCCTTATCAATAGCTCTGGCACTGTTAATGTTGATGGTTCAAACAACTTTGCCTCAGTTTTCTCCAAAAGAGGCAAGAAAGGAGTTAACCAAGATTGTTGCTTAG

Protein sequence

>MsG0180004620.01.T01

MGHFSSMFNGLAKSLKMKKGSTKYSGREAAEALAKEAKEITPLSIALALLMLMVQTTLPQFSPKEARKELTKIVA*