AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0076310

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0076310 MtrunA17_Chr1g0185261 78.601 243 30 2 1 243 1 221 3.26e-119 338
Msa0076310 MtrunA17_Chr5g0445881 34.156 243 85 6 16 186 61 300 7.46e-21 90.9
Msa0076310 MtrunA17_Chr5g0446181 39.111 225 94 9 16 215 79 285 5.08e-20 87.8
Msa0076310 MtrunA17_Chr5g0445861 49.580 119 47 2 70 175 240 358 2.16e-17 80.5
Msa0076310 MtrunA17_Chr5g0445961 58.025 81 33 1 106 185 320 400 3.01e-17 80.5
Msa0076310 MtrunA17_Chr5g0445831 38.021 192 76 5 16 167 57 245 4.66e-15 73.9
Msa0076310 MtrunA17_Chr5g0445771 41.667 132 56 4 68 187 184 306 7.55e-13 67.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 75 sgRNAs with CRISPR-Local

Find 190 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTATCTTTCTTGAGCTATTT+TGG 0.110071 1_2:+52292001 None:intergenic
GGGGTTGTCCTCTTCCTTGC+AGG 0.198536 1_2:-52291508 Msa0076310:CDS
CTTGAAAGATGTTATCAAAA+TGG 0.217383 1_2:+52292192 None:intergenic
GATCCTATGGTTGCTGCTTC+TGG 0.233693 1_2:-52291585 Msa0076310:CDS
TTCAAAGAACTCAAAATAAT+TGG 0.260116 1_2:-52291969 Msa0076310:CDS
GTACAAATTTGTCCTCATTT+TGG 0.267038 1_2:-52292276 Msa0076310:five_prime_UTR
AAAATAATTGGCTCAACTTA+TGG 0.294742 1_2:-52291957 Msa0076310:CDS
AACAAAGAATGTTGAATTAG+AGG 0.303948 1_2:-52291808 Msa0076310:CDS
GGAAACTGTGAAGAAGAATT+TGG 0.316676 1_2:-52291787 Msa0076310:CDS
AGTCTGTGGACACGAAGTTC+AGG 0.338708 1_2:-52291692 Msa0076310:CDS
TTGAAAGATGTTATCAAAAT+GGG 0.368916 1_2:+52292193 None:intergenic
CAGACTAGAGGAGCTTTCAT+TGG 0.371001 1_2:-52292090 Msa0076310:CDS
TGAGTTCTTTGAAGAGATCT+TGG 0.373162 1_2:+52291979 None:intergenic
GGGAGCTTGGTCAAGCGAGC+AGG 0.405630 1_2:-52289705 Msa0076310:CDS
TCCATTCACAGACTTTGAAA+TGG 0.406297 1_2:-52292147 Msa0076310:exon
ATAAAGCAGAAAAGAGGAAA+AGG 0.411906 1_2:-52289726 Msa0076310:CDS
TTTCTTTGTTAAAAGTTCAA+TGG 0.428054 1_2:+52291929 None:intergenic
CACTTGTACTGAGAAAGATT+TGG 0.437573 1_2:-52289537 Msa0076310:three_prime_UTR
AAGTGAAGCGGCCCTTCAAA+TGG 0.442528 1_2:+52292116 None:intergenic
AGAGACAATGGAGTTTGCTC+TGG 0.451514 1_2:-52289646 Msa0076310:three_prime_UTR
TCGATTTGTGCTTGGTTGGT+TGG 0.452174 1_2:+52292219 None:intergenic
ATGGAGATACTTCCATTTGA+AGG 0.456578 1_2:-52292128 Msa0076310:CDS
TTAATGAGAGATTGATATAG+TGG 0.461277 1_2:-52289463 Msa0076310:three_prime_UTR
TTGTGCTTGGTTGGTTGGAT+TGG 0.463061 1_2:+52292224 None:intergenic
GAGGAAAGAAAACAGCAAAC+TGG 0.467949 1_2:-52291748 Msa0076310:CDS
GGAGCTTGGTCAAGCGAGCA+GGG 0.474005 1_2:-52289704 Msa0076310:CDS
TCCATTTCAAAGTCTGTGAA+TGG 0.474011 1_2:+52292146 None:intergenic
GGGTTGTCCTCTTCCTTGCA+GGG 0.478879 1_2:-52291507 Msa0076310:intron
GTATAAAGGTGGTACTATGA+TGG 0.480771 1_2:-52289582 Msa0076310:three_prime_UTR
AGAGTTCGCTAAAAGAGAAA+AGG 0.488955 1_2:-52292039 Msa0076310:CDS
ATCTACACTCATCAAACGTT+CGG 0.489204 1_2:+52291632 None:intergenic
TTCAGAAATGGCGCTGTGGA+GGG 0.500430 1_2:-52291528 Msa0076310:CDS
TAAAGCAGAAAAGAGGAAAA+GGG 0.510608 1_2:-52289725 Msa0076310:CDS
GTTATGTTGAAGAAGAGTAA+AGG 0.515053 1_2:-52289675 Msa0076310:CDS
GAAAAGAGGAAAAGGGAGCT+TGG 0.519790 1_2:-52289718 Msa0076310:CDS
GGAGTCTGATGTTGATCCTA+TGG 0.524968 1_2:-52291598 Msa0076310:CDS
GAACTCTGACTCAACATGTA+TGG 0.529103 1_2:+52292055 None:intergenic
GTCTGTGGACACGAAGTTCA+GGG 0.534295 1_2:-52291691 Msa0076310:CDS
TAAAGGCTGTTGAGAGACAA+TGG 0.538069 1_2:-52289658 Msa0076310:exon
GCATGAAAGAGTAGCGAGGT+TGG 0.539040 1_2:-52291664 Msa0076310:CDS
GTTCAGAAATGGCGCTGTGG+AGG 0.545156 1_2:-52291529 Msa0076310:CDS
GATGACTGTGAGTTCAGAAA+TGG 0.547831 1_2:-52291540 Msa0076310:CDS
ACTCAACATGTATGGTTGAA+AGG 0.549288 1_2:+52292063 None:intergenic
TTGAAGGGTGTTGATGATGA+TGG 0.554326 1_2:-52291876 Msa0076310:CDS
AGCTTGGTCAAGCGAGCAGG+GGG 0.560859 1_2:-52289702 Msa0076310:CDS
TGGAGATACTTCCATTTGAA+GGG 0.564385 1_2:-52292127 Msa0076310:CDS
AGACTCACCCTGCAAGGAAG+AGG 0.565003 1_2:+52291500 None:intergenic
GGGTCAAGTCAAGAAATTGA+AGG 0.565533 1_2:-52291892 Msa0076310:CDS
TTCTGGAGACGAAGATGAAG+AGG 0.569256 1_2:-52291568 Msa0076310:CDS
TCAGAAATGGCGCTGTGGAG+GGG 0.569883 1_2:-52291527 Msa0076310:CDS
AAATCTATAAAGCAGAAAAG+AGG 0.570733 1_2:-52289732 Msa0076310:CDS
TGAGAAATTGCTCAAGTCTG+TGG 0.574071 1_2:-52291706 Msa0076310:CDS
AGATGGAGACTCACCCTGCA+AGG 0.586923 1_2:+52291494 None:intergenic
TTTCAAAGTCTGTGAATGGA+AGG 0.589355 1_2:+52292150 None:intergenic
AACTGGAAGATGAGAAACGC+GGG 0.589875 1_2:-52291731 Msa0076310:CDS
TCTCCAGAAGCAGCAACCAT+AGG 0.593805 1_2:+52291582 None:intergenic
GAGCTTGGTCAAGCGAGCAG+GGG 0.596255 1_2:-52289703 Msa0076310:CDS
GATATAGTGGGACCATCTGA+AGG 0.603383 1_2:-52289450 Msa0076310:three_prime_UTR
ACCACTTCACTACCAAAATG+AGG 0.604692 1_2:+52292264 None:intergenic
CAAAGTCTGTGAATGGAAGG+TGG 0.611681 1_2:+52292153 None:intergenic
GCTTCACTTTGACAGACTAG+AGG 0.616458 1_2:-52292102 Msa0076310:CDS
TCTAGTCTGTCAAAGTGAAG+CGG 0.619726 1_2:+52292104 None:intergenic
TGAAAGAGTAGCGAGGTTGG+AGG 0.624900 1_2:-52291661 Msa0076310:CDS
TAATGAGAGATTGATATAGT+GGG 0.635563 1_2:-52289462 Msa0076310:three_prime_UTR
ATGAGTGTAGATGACTCTGA+AGG 0.643207 1_2:-52291621 Msa0076310:CDS
GGTCAAGTCAAGAAATTGAA+GGG 0.648822 1_2:-52291891 Msa0076310:CDS
ATGTCTGCAAGACCTTCAGA+TGG 0.654397 1_2:+52289438 None:intergenic
TCTGTGGACACGAAGTTCAG+GGG 0.655793 1_2:-52291690 Msa0076310:CDS
TGAGTGTAGATGACTCTGAA+GGG 0.681333 1_2:-52291620 Msa0076310:CDS
TGGCAACTATAAGCGAGCTG+AGG 0.694355 1_2:-52291767 Msa0076310:CDS
GAGTGTAGATGACTCTGAAG+GGG 0.702524 1_2:-52291619 Msa0076310:CDS
TGAGTTCAGAAATGGCGCTG+TGG 0.712136 1_2:-52291532 Msa0076310:CDS
GACTGCATGAAAGAGTAGCG+AGG 0.721557 1_2:-52291668 Msa0076310:CDS
AAACTGGAAGATGAGAAACG+CGG 0.745275 1_2:-52291732 Msa0076310:CDS
ACTGGAAGATGAGAAACGCG+GGG 0.746758 1_2:-52291730 Msa0076310:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTTCAAATAAGATTATA+GGG + chr1_2:52290465-52290484 None:intergenic 10.0%
!!! TTTTTTTCAAATAAGATTAT+AGG + chr1_2:52290466-52290485 None:intergenic 10.0%
!!! ATCATAAGTATTTTTTTTAG+AGG - chr1_2:52291477-52291496 Msa0076310:intron 15.0%
!!! ATCTACTATATTTTGTATAA+AGG - chr1_2:52292056-52292075 Msa0076310:CDS 15.0%
!! TTGAAAGATGTTATCAAAAT+GGG + chr1_2:52289462-52289481 None:intergenic 20.0%
!! TTCAAAGAACTCAAAATAAT+TGG - chr1_2:52289683-52289702 Msa0076310:CDS 20.0%
!! TTTCTTTGTTAAAAGTTCAA+TGG + chr1_2:52289726-52289745 None:intergenic 20.0%
!! TATTTATATTAGGATCTGTT+TGG - chr1_2:52290244-52290263 Msa0076310:intron 20.0%
!! TATTTGTGATCATATTGATA+AGG - chr1_2:52290774-52290793 Msa0076310:intron 20.0%
!! ATTTGTGATCATATTGATAA+GGG - chr1_2:52290775-52290794 Msa0076310:intron 20.0%
!! TAATTGCTACATAATTAAAC+TGG + chr1_2:52290861-52290880 None:intergenic 20.0%
!!! ATAATACCTAATGATGTTTT+AGG + chr1_2:52291310-52291329 None:intergenic 20.0%
!! ACAAATTAAGTCATACAATA+TGG + chr1_2:52291755-52291774 None:intergenic 20.0%
!!! TACTATATTTTGTATAAAGG+TGG - chr1_2:52292059-52292078 Msa0076310:CDS 20.0%
! CTTGAAAGATGTTATCAAAA+TGG + chr1_2:52289463-52289482 None:intergenic 25.0%
!! AAAATAATTGGCTCAACTTA+TGG - chr1_2:52289695-52289714 Msa0076310:CDS 25.0%
! AAAGAAAAACAATGAATTGC+AGG - chr1_2:52289739-52289758 Msa0076310:intron 25.0%
! AAGAAAAACAATGAATTGCA+GGG - chr1_2:52289740-52289759 Msa0076310:intron 25.0%
! AACAAAGAATGTTGAATTAG+AGG - chr1_2:52289844-52289863 Msa0076310:intron 25.0%
! ACTGATAAGTTAACTTATAG+CGG + chr1_2:52290639-52290658 None:intergenic 25.0%
! ATTCTAGTGTAAAGACATTT+AGG + chr1_2:52290688-52290707 None:intergenic 25.0%
! TCATATTGATAAGGGTAAAT+TGG - chr1_2:52290783-52290802 Msa0076310:intron 25.0%
! CATATTGATAAGGGTAAATT+GGG - chr1_2:52290784-52290803 Msa0076310:intron 25.0%
! ATATGATAGAATAGAAGTTC+TGG - chr1_2:52290910-52290929 Msa0076310:intron 25.0%
!! GTTATCACTCAATTAACTTT+TGG + chr1_2:52290942-52290961 None:intergenic 25.0%
! AGTGATAACAGATAATAGTA+TGG - chr1_2:52290953-52290972 Msa0076310:intron 25.0%
! ATCATAATACACCAATAACA+AGG + chr1_2:52291025-52291044 None:intergenic 25.0%
! AATTGACTGTACATTTGAAT+TGG + chr1_2:52291191-52291210 None:intergenic 25.0%
! CATCATTAGGTATTATCATT+CGG - chr1_2:52291314-52291333 Msa0076310:intron 25.0%
! TGTTGTTAGCATATTATTAG+AGG - chr1_2:52291508-52291527 Msa0076310:CDS 25.0%
! GTTGTTAGCATATTATTAGA+GGG - chr1_2:52291509-52291528 Msa0076310:CDS 25.0%
! TTGTTAGCATATTATTAGAG+GGG - chr1_2:52291510-52291529 Msa0076310:CDS 25.0%
! AGTATGAAGCTGTAATAATA+TGG + chr1_2:52291810-52291829 None:intergenic 25.0%
! ACTAGTAAATAAGCACAATA+AGG + chr1_2:52291876-52291895 None:intergenic 25.0%
! AAATCTATAAAGCAGAAAAG+AGG - chr1_2:52291920-52291939 Msa0076310:CDS 25.0%
! TTAATGAGAGATTGATATAG+TGG - chr1_2:52292189-52292208 Msa0076310:five_prime_UTR 25.0%
! TAATGAGAGATTGATATAGT+GGG - chr1_2:52292190-52292209 Msa0076310:five_prime_UTR 25.0%
! GTACAAATTTGTCCTCATTT+TGG - chr1_2:52289376-52289395 Msa0076310:three_prime_UTR 30.0%
! GTATCTTTCTTGAGCTATTT+TGG + chr1_2:52289654-52289673 None:intergenic 30.0%
ACAACACAAACACTAATTGT+GGG + chr1_2:52290218-52290237 None:intergenic 30.0%
ACAATTAGTGTTTGTGTTGT+GGG - chr1_2:52290218-52290237 Msa0076310:intron 30.0%
TTGTGGGCTCTATTTATATT+AGG - chr1_2:52290234-52290253 Msa0076310:intron 30.0%
AATGTCTTTACACTAGAATG+TGG - chr1_2:52290689-52290708 Msa0076310:intron 30.0%
AAAACCAAGGTTTCATATCT+TGG + chr1_2:52290727-52290746 None:intergenic 30.0%
ATTAGATGCCATTCACAAAA+TGG + chr1_2:52290892-52290911 None:intergenic 30.0%
!! ACTGTTTTAGATTCAGTTGT+TGG - chr1_2:52291046-52291065 Msa0076310:intron 30.0%
!! CTGTTTTAGATTCAGTTGTT+GGG - chr1_2:52291047-52291066 Msa0076310:intron 30.0%
!! TGTTTTAGATTCAGTTGTTG+GGG - chr1_2:52291048-52291067 Msa0076310:intron 30.0%
CTTTGAATGTACTCTGATTT+AGG + chr1_2:52291162-52291181 None:intergenic 30.0%
CTAAATCAGAGTACATTCAA+AGG - chr1_2:52291160-52291179 Msa0076310:intron 30.0%
GCATAACCTAAAACATCATT+AGG - chr1_2:52291301-52291320 Msa0076310:intron 30.0%
ACAATATGGTCAAACAAAGA+CGG + chr1_2:52291741-52291760 None:intergenic 30.0%
ATAAAGCAGAAAAGAGGAAA+AGG - chr1_2:52291926-52291945 Msa0076310:CDS 30.0%
TAAAGCAGAAAAGAGGAAAA+GGG - chr1_2:52291927-52291946 Msa0076310:CDS 30.0%
GTTATGTTGAAGAAGAGTAA+AGG - chr1_2:52291977-52291996 Msa0076310:CDS 30.0%
TTTCAAAGTCTGTGAATGGA+AGG + chr1_2:52289505-52289524 None:intergenic 35.0%
! TCCATTCACAGACTTTGAAA+TGG - chr1_2:52289505-52289524 Msa0076310:three_prime_UTR 35.0%
TCCATTTCAAAGTCTGTGAA+TGG + chr1_2:52289509-52289528 None:intergenic 35.0%
ATGGAGATACTTCCATTTGA+AGG - chr1_2:52289524-52289543 Msa0076310:three_prime_UTR 35.0%
TGGAGATACTTCCATTTGAA+GGG - chr1_2:52289525-52289544 Msa0076310:three_prime_UTR 35.0%
ACTCAACATGTATGGTTGAA+AGG + chr1_2:52289592-52289611 None:intergenic 35.0%
AGAGTTCGCTAAAAGAGAAA+AGG - chr1_2:52289613-52289632 Msa0076310:three_prime_UTR 35.0%
! TGAGTTCTTTGAAGAGATCT+TGG + chr1_2:52289676-52289695 None:intergenic 35.0%
GGTCAAGTCAAGAAATTGAA+GGG - chr1_2:52289761-52289780 Msa0076310:intron 35.0%
GGAAACTGTGAAGAAGAATT+TGG - chr1_2:52289865-52289884 Msa0076310:intron 35.0%
ATCTACACTCATCAAACGTT+CGG + chr1_2:52290023-52290042 None:intergenic 35.0%
CAACACAAACACTAATTGTG+GGG + chr1_2:52290217-52290236 None:intergenic 35.0%
CACAACACAAACACTAATTG+TGG + chr1_2:52290219-52290238 None:intergenic 35.0%
CACAATTAGTGTTTGTGTTG+TGG - chr1_2:52290217-52290236 Msa0076310:intron 35.0%
! GCTTTAAGCTATTAGCTACT+TGG + chr1_2:52290405-52290424 None:intergenic 35.0%
!! TATAGCGGATGAACTTATAG+TGG + chr1_2:52290624-52290643 None:intergenic 35.0%
AAAGACATTTAGGCTCTGTT+TGG + chr1_2:52290678-52290697 None:intergenic 35.0%
TCATCCAAGATATGAAACCT+TGG - chr1_2:52290720-52290739 Msa0076310:intron 35.0%
TGAATGAACTAGCAAAACCA+AGG + chr1_2:52290740-52290759 None:intergenic 35.0%
GTAAATTGGGCTACATCTAA+GGG - chr1_2:52290797-52290816 Msa0076310:intron 35.0%
! CTTGAAGACCATTTTGTGAA+TGG - chr1_2:52290881-52290900 Msa0076310:intron 35.0%
!! ATTTGATGATGACCCAATTG+TGG - chr1_2:52290991-52291010 Msa0076310:intron 35.0%
AAGGAATCACTTCCACAATT+GGG + chr1_2:52291006-52291025 None:intergenic 35.0%
! TGGAAGTGATTCCTTGTTAT+TGG - chr1_2:52291011-52291030 Msa0076310:intron 35.0%
TTACTGAAGGTCAAGATAGA+AGG + chr1_2:52291080-52291099 None:intergenic 35.0%
TTAGCATATTATTAGAGGGG+AGG - chr1_2:52291513-52291532 Msa0076310:CDS 35.0%
TAGCATATTATTAGAGGGGA+GGG - chr1_2:52291514-52291533 Msa0076310:CDS 35.0%
TTAATGAACTTCATGTCTGC+AGG - chr1_2:52291896-52291915 Msa0076310:CDS 35.0%
!!! GTTTTGATTCTTGATATGCG+AGG - chr1_2:52292028-52292047 Msa0076310:CDS 35.0%
GTATAAAGGTGGTACTATGA+TGG - chr1_2:52292070-52292089 Msa0076310:CDS 35.0%
CACTTGTACTGAGAAAGATT+TGG - chr1_2:52292115-52292134 Msa0076310:CDS 35.0%
!!! TCCTCATTTTGGTAGTGAAG+TGG - chr1_2:52289387-52289406 Msa0076310:three_prime_UTR 40.0%
ACCACTTCACTACCAAAATG+AGG + chr1_2:52289391-52289410 None:intergenic 40.0%
!!! ATTTTGGTAGTGAAGTGGTG+TGG - chr1_2:52289392-52289411 Msa0076310:three_prime_UTR 40.0%
!! GTTTTCGATTTGTGCTTGGT+TGG + chr1_2:52289440-52289459 None:intergenic 40.0%
!! ATGGGTTTTCGATTTGTGCT+TGG + chr1_2:52289444-52289463 None:intergenic 40.0%
GGAAGGTGGAAGTGAAAAAA+GGG + chr1_2:52289488-52289507 None:intergenic 40.0%
TGGAAGGTGGAAGTGAAAAA+AGG + chr1_2:52289489-52289508 None:intergenic 40.0%
TCTAGTCTGTCAAAGTGAAG+CGG + chr1_2:52289551-52289570 None:intergenic 40.0%
GAACTCTGACTCAACATGTA+TGG + chr1_2:52289600-52289619 None:intergenic 40.0%
GGGTCAAGTCAAGAAATTGA+AGG - chr1_2:52289760-52289779 Msa0076310:intron 40.0%
!! TTGAAGGGTGTTGATGATGA+TGG - chr1_2:52289776-52289795 Msa0076310:intron 40.0%
GAGGAAAGAAAACAGCAAAC+TGG - chr1_2:52289904-52289923 Msa0076310:intron 40.0%
AAACTGGAAGATGAGAAACG+CGG - chr1_2:52289920-52289939 Msa0076310:intron 40.0%
TGAGAAATTGCTCAAGTCTG+TGG - chr1_2:52289946-52289965 Msa0076310:intron 40.0%
ATGAGTGTAGATGACTCTGA+AGG - chr1_2:52290031-52290050 Msa0076310:intron 40.0%
TGAGTGTAGATGACTCTGAA+GGG - chr1_2:52290032-52290051 Msa0076310:intron 40.0%
GATGACTGTGAGTTCAGAAA+TGG - chr1_2:52290112-52290131 Msa0076310:intron 40.0%
GGTAAATTGGGCTACATCTA+AGG - chr1_2:52290796-52290815 Msa0076310:intron 40.0%
CAAGGAATCACTTCCACAAT+TGG + chr1_2:52291007-52291026 None:intergenic 40.0%
TCTTGCATTGCACTTACTGA+AGG + chr1_2:52291093-52291112 None:intergenic 40.0%
TCAGTAAGTGCAATGCAAGA+TGG - chr1_2:52291093-52291112 Msa0076310:intron 40.0%
ATACAGTTGTGGTAGACAGA+TGG - chr1_2:52291130-52291149 Msa0076310:intron 40.0%
GTTCTTGGTCAAGCTATACT+TGG - chr1_2:52291367-52291386 Msa0076310:intron 40.0%
TTAAAGGATTGGGACGTTGA+AGG + chr1_2:52291575-52291594 None:intergenic 40.0%
AGCCTAGTGGTTAAAGGATT+GGG + chr1_2:52291585-52291604 None:intergenic 40.0%
TAGCCTAGTGGTTAAAGGAT+TGG + chr1_2:52291586-52291605 None:intergenic 40.0%
TGATTTAGCCTCATATCCCT+TGG - chr1_2:52291649-52291668 Msa0076310:CDS 40.0%
GATTTAGCCTCATATCCCTT+GGG - chr1_2:52291650-52291669 Msa0076310:CDS 40.0%
CTTTCCTTGACACAAAGCTT+TGG + chr1_2:52291681-52291700 None:intergenic 40.0%
TAAAGGCTGTTGAGAGACAA+TGG - chr1_2:52291994-52292013 Msa0076310:CDS 40.0%
! TTGTGCTTGGTTGGTTGGAT+TGG + chr1_2:52289431-52289450 None:intergenic 45.0%
! TCGATTTGTGCTTGGTTGGT+TGG + chr1_2:52289436-52289455 None:intergenic 45.0%
CAAAGTCTGTGAATGGAAGG+TGG + chr1_2:52289502-52289521 None:intergenic 45.0%
GCTTCACTTTGACAGACTAG+AGG - chr1_2:52289550-52289569 Msa0076310:three_prime_UTR 45.0%
CAGACTAGAGGAGCTTTCAT+TGG - chr1_2:52289562-52289581 Msa0076310:three_prime_UTR 45.0%
AACTGGAAGATGAGAAACGC+GGG - chr1_2:52289921-52289940 Msa0076310:intron 45.0%
! GAGTGTAGATGACTCTGAAG+GGG - chr1_2:52290033-52290052 Msa0076310:intron 45.0%
GGAGTCTGATGTTGATCCTA+TGG - chr1_2:52290054-52290073 Msa0076310:intron 45.0%
! TTCTGGAGACGAAGATGAAG+AGG - chr1_2:52290084-52290103 Msa0076310:intron 45.0%
!! TCCATCTCTAGGATGTGCAT+AGG - chr1_2:52290174-52290193 Msa0076310:intron 45.0%
ACCTATGCACATCCTAGAGA+TGG + chr1_2:52290178-52290197 None:intergenic 45.0%
ATAAGCTCGTGAGAATGCGA+TGG + chr1_2:52290340-52290359 None:intergenic 45.0%
AATTGGGCTACATCTAAGGG+TGG - chr1_2:52290800-52290819 Msa0076310:intron 45.0%
ATTGGGCTACATCTAAGGGT+GGG - chr1_2:52290801-52290820 Msa0076310:intron 45.0%
CACAACTGTATTGCACGACA+AGG + chr1_2:52291121-52291140 None:intergenic 45.0%
CTTGTCGTGCAATACAGTTG+TGG - chr1_2:52291119-52291138 Msa0076310:intron 45.0%
GAGAATGGGTCCTAGTAATC+CGG + chr1_2:52291415-52291434 None:intergenic 45.0%
ATTACTAGGACCCATTCTCC+TGG - chr1_2:52291416-52291435 Msa0076310:intron 45.0%
TCTCCGATTTCCAGGAGAAT+GGG + chr1_2:52291429-52291448 None:intergenic 45.0%
AGGGGAGGGTAAAATGTTAG+CGG - chr1_2:52291528-52291547 Msa0076310:CDS 45.0%
GTCCCAATCCTTTAACCACT+AGG - chr1_2:52291580-52291599 Msa0076310:CDS 45.0%
AGGTGTAGCCTAGTGGTTAA+AGG + chr1_2:52291591-52291610 None:intergenic 45.0%
TACCTATGATGTGTTCGCGA+AGG + chr1_2:52291611-52291630 None:intergenic 45.0%
! AAAGCTTTGGCTGAACCCAA+GGG + chr1_2:52291668-52291687 None:intergenic 45.0%
! TCAGCCAAAGCTTTGTGTCA+AGG - chr1_2:52291674-52291693 Msa0076310:CDS 45.0%
GAAAAGAGGAAAAGGGAGCT+TGG - chr1_2:52291934-52291953 Msa0076310:CDS 45.0%
! AGAGACAATGGAGTTTGCTC+TGG - chr1_2:52292006-52292025 Msa0076310:CDS 45.0%
! GATATAGTGGGACCATCTGA+AGG - chr1_2:52292202-52292221 Msa0076310:five_prime_UTR 45.0%
ATGTCTGCAAGACCTTCAGA+TGG + chr1_2:52292217-52292236 None:intergenic 45.0%
!!! ATGTTTAATATTTATTTTTT+TGG + chr1_2:52291459-52291478 None:intergenic 5.0%
AAGTGAAGCGGCCCTTCAAA+TGG + chr1_2:52289539-52289558 None:intergenic 50.0%
TGGCAACTATAAGCGAGCTG+AGG - chr1_2:52289885-52289904 Msa0076310:intron 50.0%
ACTGGAAGATGAGAAACGCG+GGG - chr1_2:52289922-52289941 Msa0076310:intron 50.0%
AGTCTGTGGACACGAAGTTC+AGG - chr1_2:52289960-52289979 Msa0076310:intron 50.0%
GTCTGTGGACACGAAGTTCA+GGG - chr1_2:52289961-52289980 Msa0076310:intron 50.0%
TCTGTGGACACGAAGTTCAG+GGG - chr1_2:52289962-52289981 Msa0076310:intron 50.0%
GACTGCATGAAAGAGTAGCG+AGG - chr1_2:52289984-52290003 Msa0076310:intron 50.0%
GCATGAAAGAGTAGCGAGGT+TGG - chr1_2:52289988-52290007 Msa0076310:intron 50.0%
TGAAAGAGTAGCGAGGTTGG+AGG - chr1_2:52289991-52290010 Msa0076310:intron 50.0%
! GATCCTATGGTTGCTGCTTC+TGG - chr1_2:52290067-52290086 Msa0076310:intron 50.0%
TCTCCAGAAGCAGCAACCAT+AGG + chr1_2:52290073-52290092 None:intergenic 50.0%
TGAGTTCAGAAATGGCGCTG+TGG - chr1_2:52290120-52290139 Msa0076310:intron 50.0%
TTCAGAAATGGCGCTGTGGA+GGG - chr1_2:52290124-52290143 Msa0076310:intron 50.0%
AAAACCCGAGTTCGTTTCCG+AGG - chr1_2:52291337-52291356 Msa0076310:intron 50.0%
TTCCACCTCGGAAACGAACT+CGG + chr1_2:52291345-52291364 None:intergenic 50.0%
TTCCGAGGTGGAACTGTTCT+TGG - chr1_2:52291352-52291371 Msa0076310:intron 50.0%
GACCAAGAACAGTTCCACCT+CGG + chr1_2:52291357-52291376 None:intergenic 50.0%
GGACCCATTCTCCTGGAAAT+CGG - chr1_2:52291423-52291442 Msa0076310:intron 50.0%
CTCTCCGATTTCCAGGAGAA+TGG + chr1_2:52291430-52291449 None:intergenic 50.0%
TCTCCTGGAAATCGGAGAGT+TGG - chr1_2:52291431-52291450 Msa0076310:intron 50.0%
AAGGATTGGGACGTTGAAGG+AGG + chr1_2:52291572-52291591 None:intergenic 50.0%
CACCTTCGCGAACACATCAT+AGG - chr1_2:52291606-52291625 Msa0076310:CDS 50.0%
! CAAAGCTTTGGCTGAACCCA+AGG + chr1_2:52291669-52291688 None:intergenic 50.0%
GTTCAGAAATGGCGCTGTGG+AGG - chr1_2:52290123-52290142 Msa0076310:intron 55.0%
TCAGAAATGGCGCTGTGGAG+GGG - chr1_2:52290125-52290144 Msa0076310:intron 55.0%
GGGTTGTCCTCTTCCTTGCA+GGG - chr1_2:52290145-52290164 Msa0076310:intron 55.0%
AGACTCACCCTGCAAGGAAG+AGG + chr1_2:52290155-52290174 None:intergenic 55.0%
AGATGGAGACTCACCCTGCA+AGG + chr1_2:52290161-52290180 None:intergenic 55.0%
CAGGGTGAGTCTCCATCTCT+AGG - chr1_2:52290163-52290182 Msa0076310:intron 55.0%
TCCACCTCGGAAACGAACTC+GGG + chr1_2:52291344-52291363 None:intergenic 55.0%
CTTACCTCTTGACCGAGCTC+CGG - chr1_2:52291393-52291412 Msa0076310:intron 55.0%
TAATCCGGAGCTCGGTCAAG+AGG + chr1_2:52291400-52291419 None:intergenic 55.0%
TGACCGAGCTCCGGATTACT+AGG - chr1_2:52291402-52291421 Msa0076310:intron 55.0%
GGTCCTAGTAATCCGGAGCT+CGG + chr1_2:52291408-52291427 None:intergenic 55.0%
GTGCCAACTCTCCGATTTCC+AGG + chr1_2:52291437-52291456 None:intergenic 55.0%
GGGACGTTGAAGGAGGTTCA+AGG + chr1_2:52291565-52291584 None:intergenic 55.0%
! TTCGCGAAGGTGTAGCCTAG+TGG + chr1_2:52291598-52291617 None:intergenic 55.0%
GGCTGAACCCAAGGGATATG+AGG + chr1_2:52291660-52291679 None:intergenic 55.0%
GGGGTTGTCCTCTTCCTTGC+AGG - chr1_2:52290144-52290163 Msa0076310:intron 60.0%
ACCCGAGTTCGTTTCCGAGG+TGG - chr1_2:52291340-52291359 Msa0076310:intron 60.0%
GGAGCTTGGTCAAGCGAGCA+GGG - chr1_2:52291948-52291967 Msa0076310:CDS 60.0%
GAGCTTGGTCAAGCGAGCAG+GGG - chr1_2:52291949-52291968 Msa0076310:CDS 60.0%
AGCTTGGTCAAGCGAGCAGG+GGG - chr1_2:52291950-52291969 Msa0076310:CDS 60.0%
GGGAGCTTGGTCAAGCGAGC+AGG - chr1_2:52291947-52291966 Msa0076310:CDS 65.0%
Chromosome Type Strat End Strand Name
chr1_2 gene 52289363 52292311 52289363 ID=Msa0076310;Name=Msa0076310
chr1_2 mRNA 52289363 52292311 52289363 ID=Msa0076310-mRNA-1;Parent=Msa0076310;Name=Msa0076310-mRNA-1;_AED=0.01;_eAED=0.01;_QI=161|1|1|1|1|1|2|305|243
chr1_2 exon 52289363 52289756 52289363 ID=Msa0076310-mRNA-1:exon:16184;Parent=Msa0076310-mRNA-1
chr1_2 exon 52291508 52292311 52291508 ID=Msa0076310-mRNA-1:exon:16183;Parent=Msa0076310-mRNA-1
chr1_2 five_prime_UTR 52292151 52292311 52292151 ID=Msa0076310-mRNA-1:five_prime_utr;Parent=Msa0076310-mRNA-1
chr1_2 CDS 52291508 52292150 52291508 ID=Msa0076310-mRNA-1:cds;Parent=Msa0076310-mRNA-1
chr1_2 CDS 52289668 52289756 52289668 ID=Msa0076310-mRNA-1:cds;Parent=Msa0076310-mRNA-1
chr1_2 three_prime_UTR 52289363 52289667 52289363 ID=Msa0076310-mRNA-1:three_prime_utr;Parent=Msa0076310-mRNA-1
Gene Sequence

>Msa0076310

ATGGAGATACTTCCATTTGAAGGGCCGCTTCACTTTGACAGACTAGAGGAGCTTTCATTGGAGAGCCTTTCAACCATACATGTTGAGTCAGAGTTCGCTAAAAGAGAAAAGGAACTATCTTTGAAAGCCAAAATAGCTCAAGAAAGATACCAAGATCTCTTCAAAGAACTCAAAATAATTGGCTCAACTTATGGTAAAACCATTGAACTTTTAACAAAGAAAAACAATGAATTGCAGGGTCAAGTCAAGAAATTGAAGGGTGTTGATGATGATGGTAGTACTAGTAGCTATGACTATGACTACGAACTTGATGTGTTAAGAACAAAGAATGTTGAATTAGAGGAAACTGTGAAGAAGAATTTGGCAACTATAAGCGAGCTGAGGAAAGAAAACAGCAAACTGGAAGATGAGAAACGCGGGGTTGAGAAATTGCTCAAGTCTGTGGACACGAAGTTCAGGGGACTGCATGAAAGAGTAGCGAGGTTGGAGGATGATACCGAACGTTTGATGAGTGTAGATGACTCTGAAGGGGAGTCTGATGTTGATCCTATGGTTGCTGCTTCTGGAGACGAAGATGAAGAGGATGTTGATGACTGTGAGTTCAGAAATGGCGCTGTGGAGGGGTTGTCCTCTTCCTTGCAGGGCAAATCTATAAAGCAGAAAAGAGGAAAAGGGAGCTTGGTCAAGCGAGCAGGGGGAAAAGTTATGTTGAAGAAGAGTAAAGGCTGTTGA
Protein sequence

>Msa0076310

MEILPFEGPLHFDRLEELSLESLSTIHVESEFAKREKELSLKAKIAQERYQDLFKELKIIGSTYGKTIELLTKKNNELQGQVKKLKGVDDDGSTSSYDYDYELDVLRTKNVELEETVKKNLATISELRKENSKLEDEKRGVEKLLKSVDTKFRGLHERVARLEDDTERLMSVDDSEGESDVDPMVAASGDEDEEDVDDCEFRNGAVEGLSSSLQGKSIKQKRGKGSLVKRAGGKVMLKKSKGC*