AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1169090


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 31 sgRNAs with CRISPR-Local

Find 111 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TACAATTACTCGTGCTATTA+TGG 0.109957 7_4:+62033852 Msa1169090:CDS
CTGCAGGTCAAGGCTGATTT+TGG 0.138857 7_4:+62033485 Msa1169090:intron
TACTGGTGATGTTTCTAAAA+AGG 0.173626 7_4:+62033247 Msa1169090:five_prime_UTR
TCGGATTAACCAACACAATT+AGG 0.275265 7_4:+62033181 Msa1169090:five_prime_UTR
CAGTGTGCATATTGATTTAG+AGG 0.321280 7_4:+62033802 Msa1169090:CDS
TGAGTTGAGAAGGTTGAATA+AGG 0.323601 7_4:+62032980 Msa1169090:five_prime_UTR
CACTTGTAGGCTCTCTTCTA+TGG 0.338739 7_4:+62033028 Msa1169090:five_prime_UTR
ACAATTACTCGTGCTATTAT+GGG 0.418679 7_4:+62033853 Msa1169090:CDS
TTAACTTAAATCATACTGAT+AGG 0.430639 7_4:+62033356 Msa1169090:CDS
AACCAACACAATTAGGCTCT+TGG 0.438251 7_4:+62033188 Msa1169090:five_prime_UTR
GATAAGAGGAAGCGGTGAAG+GGG 0.453973 7_4:+62032913 None:intergenic
TTGTAATAGAAGATATGAAT+AGG 0.461497 7_4:+62033152 Msa1169090:five_prime_UTR
GAAGTAAATGTAAAGGAAGA+AGG 0.468064 7_4:+62033776 Msa1169090:CDS
AGCCTTGCTCTGCAAATACT+TGG 0.488191 7_4:-62033528 None:intergenic
AGATAAGAGGAAGCGGTGAA+GGG 0.491215 7_4:+62032912 None:intergenic
AGATATGAATAGGCTCTCTT+CGG 0.510356 7_4:+62033162 Msa1169090:five_prime_UTR
AGAAGAGGAACCTCACTTGT+AGG 0.517175 7_4:+62033015 Msa1169090:five_prime_UTR
GTCCAAGTATTTGCAGAGCA+AGG 0.527011 7_4:+62033526 Msa1169090:CDS
AGCCAAGAGCCTAATTGTGT+TGG 0.531992 7_4:-62033190 None:intergenic
TTACTGAGTTGGACTTAAAG+TGG 0.536061 7_4:+62033300 Msa1169090:five_prime_UTR
ATAAGAGGAAGCGGTGAAGG+GGG 0.538898 7_4:+62032914 None:intergenic
TTGAAGTTGTTGAGTTGAGA+AGG 0.559117 7_4:+62032970 Msa1169090:five_prime_UTR
GAGCAAAGAAGTAAATGTAA+AGG 0.561628 7_4:+62033769 Msa1169090:CDS
TGTATGCTATCTTACTGAGT+TGG 0.580470 7_4:+62033289 Msa1169090:five_prime_UTR
AGGAGCTGCATATGTAGAAG+AGG 0.591272 7_4:+62033000 Msa1169090:five_prime_UTR
ATGATTTAAGTTAATAACCA+TGG 0.599391 7_4:-62033347 None:intergenic
TCATACTGATAGGACAGTGA+TGG 0.631868 7_4:+62033366 Msa1169090:CDS
TAGAAGAGAGCCTACAAGTG+AGG 0.633933 7_4:-62033025 None:intergenic
TAGTTGTGCCTGCCAAAACA+CGG 0.641537 7_4:-62033226 None:intergenic
AGCAGAACATGCAATGAGTG+CGG 0.674542 7_4:+62033736 Msa1169090:CDS
TAACTTGCTGAGAAATACCA+TGG 0.684621 7_4:+62033330 Msa1169090:exon

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTGTAATAGAAGATATGAAT+AGG + chr7_4:62033152-62033171 Msa1169090:five_prime_UTR 20.0%
!!! ATGATTTAAGTTAATAACCA+TGG - chr7_4:62033350-62033369 None:intergenic 20.0%
!! TTAACTTAAATCATACTGAT+AGG + chr7_4:62033356-62033375 Msa1169090:CDS 20.0%
!! TTGTAATAGAAGATATGAAT+AGG + chr7_4:62033152-62033171 Msa1169090:five_prime_UTR 20.0%
!!! ATGATTTAAGTTAATAACCA+TGG - chr7_4:62033350-62033369 None:intergenic 20.0%
!! TTAACTTAAATCATACTGAT+AGG + chr7_4:62033356-62033375 Msa1169090:CDS 20.0%
!! TGACATAATTTCTTTTCTCA+TGG + chr7_4:62033120-62033139 Msa1169090:five_prime_UTR 25.0%
!! GACATAATTTCTTTTCTCAT+GGG + chr7_4:62033121-62033140 Msa1169090:five_prime_UTR 25.0%
!! CTTTGAATGGTAATTCATTT+TGG - chr7_4:62033425-62033444 None:intergenic 25.0%
! CAAAATGAATTACCATTCAA+AGG + chr7_4:62033423-62033442 Msa1169090:intron 25.0%
! ATTTCTAACTTAATGGTACT+TGG - chr7_4:62033555-62033574 None:intergenic 25.0%
! GTTAAAGAAAATTAGAAGAC+TGG - chr7_4:62033648-62033667 None:intergenic 25.0%
!!! TGTTTTGAAGTCAATGAAAT+TGG + chr7_4:62033713-62033732 Msa1169090:CDS 25.0%
!! TTTTTCTTTGTTGCAAAACA+TGG - chr7_4:62033830-62033849 None:intergenic 25.0%
!! TGACATAATTTCTTTTCTCA+TGG + chr7_4:62033120-62033139 Msa1169090:five_prime_UTR 25.0%
!! GACATAATTTCTTTTCTCAT+GGG + chr7_4:62033121-62033140 Msa1169090:five_prime_UTR 25.0%
!! CTTTGAATGGTAATTCATTT+TGG - chr7_4:62033425-62033444 None:intergenic 25.0%
! CAAAATGAATTACCATTCAA+AGG + chr7_4:62033423-62033442 Msa1169090:intron 25.0%
! ATTTCTAACTTAATGGTACT+TGG - chr7_4:62033555-62033574 None:intergenic 25.0%
! GTTAAAGAAAATTAGAAGAC+TGG - chr7_4:62033648-62033667 None:intergenic 25.0%
!!! TGTTTTGAAGTCAATGAAAT+TGG + chr7_4:62033713-62033732 Msa1169090:CDS 25.0%
!! TTTTTCTTTGTTGCAAAACA+TGG - chr7_4:62033830-62033849 None:intergenic 25.0%
! TACTGGTGATGTTTCTAAAA+AGG + chr7_4:62033247-62033266 Msa1169090:five_prime_UTR 30.0%
! ACTGGTGATGTTTCTAAAAA+GGG + chr7_4:62033248-62033267 Msa1169090:five_prime_UTR 30.0%
! AGTACTTGATAACCTTTGAA+TGG - chr7_4:62033438-62033457 None:intergenic 30.0%
GTACCATTAAGTTAGAAATG+CGG + chr7_4:62033556-62033575 Msa1169090:intron 30.0%
TACCATTAAGTTAGAAATGC+GGG + chr7_4:62033557-62033576 Msa1169090:intron 30.0%
TAAAAAGTAAACAAGCAGCA+AGG - chr7_4:62033597-62033616 None:intergenic 30.0%
!!! TTGCTGCTTGTTTACTTTTT+AGG + chr7_4:62033596-62033615 Msa1169090:intron 30.0%
GAGCAAAGAAGTAAATGTAA+AGG + chr7_4:62033769-62033788 Msa1169090:CDS 30.0%
GAAGTAAATGTAAAGGAAGA+AGG + chr7_4:62033776-62033795 Msa1169090:CDS 30.0%
! TACAATTACTCGTGCTATTA+TGG + chr7_4:62033852-62033871 Msa1169090:CDS 30.0%
! ACAATTACTCGTGCTATTAT+GGG + chr7_4:62033853-62033872 Msa1169090:CDS 30.0%
! TACTGGTGATGTTTCTAAAA+AGG + chr7_4:62033247-62033266 Msa1169090:five_prime_UTR 30.0%
! ACTGGTGATGTTTCTAAAAA+GGG + chr7_4:62033248-62033267 Msa1169090:five_prime_UTR 30.0%
! AGTACTTGATAACCTTTGAA+TGG - chr7_4:62033438-62033457 None:intergenic 30.0%
GTACCATTAAGTTAGAAATG+CGG + chr7_4:62033556-62033575 Msa1169090:intron 30.0%
TACCATTAAGTTAGAAATGC+GGG + chr7_4:62033557-62033576 Msa1169090:intron 30.0%
TAAAAAGTAAACAAGCAGCA+AGG - chr7_4:62033597-62033616 None:intergenic 30.0%
!!! TTGCTGCTTGTTTACTTTTT+AGG + chr7_4:62033596-62033615 Msa1169090:intron 30.0%
GAGCAAAGAAGTAAATGTAA+AGG + chr7_4:62033769-62033788 Msa1169090:CDS 30.0%
GAAGTAAATGTAAAGGAAGA+AGG + chr7_4:62033776-62033795 Msa1169090:CDS 30.0%
! TACAATTACTCGTGCTATTA+TGG + chr7_4:62033852-62033871 Msa1169090:CDS 30.0%
! ACAATTACTCGTGCTATTAT+GGG + chr7_4:62033853-62033872 Msa1169090:CDS 30.0%
!! TTGAAGTTGTTGAGTTGAGA+AGG + chr7_4:62032970-62032989 Msa1169090:five_prime_UTR 35.0%
! TGAGTTGAGAAGGTTGAATA+AGG + chr7_4:62032980-62032999 Msa1169090:five_prime_UTR 35.0%
AGATATGAATAGGCTCTCTT+CGG + chr7_4:62033162-62033181 Msa1169090:five_prime_UTR 35.0%
TCGGATTAACCAACACAATT+AGG + chr7_4:62033181-62033200 Msa1169090:five_prime_UTR 35.0%
!! TTTTGATTGATGCCGTGTTT+TGG + chr7_4:62033214-62033233 Msa1169090:five_prime_UTR 35.0%
! ATGTTTCTAAAAAGGGTCGA+TGG + chr7_4:62033255-62033274 Msa1169090:five_prime_UTR 35.0%
TGTATGCTATCTTACTGAGT+TGG + chr7_4:62033289-62033308 Msa1169090:five_prime_UTR 35.0%
! TTACTGAGTTGGACTTAAAG+TGG + chr7_4:62033300-62033319 Msa1169090:five_prime_UTR 35.0%
!! TAACTTGCTGAGAAATACCA+TGG + chr7_4:62033330-62033349 Msa1169090:exon 35.0%
TTTGGACAGAGTAAACTTCA+AGG - chr7_4:62033407-62033426 None:intergenic 35.0%
AACCCGCATTTCTAACTTAA+TGG - chr7_4:62033562-62033581 None:intergenic 35.0%
!! CAGTGTGCATATTGATTTAG+AGG + chr7_4:62033802-62033821 Msa1169090:CDS 35.0%
!! TTGAAGTTGTTGAGTTGAGA+AGG + chr7_4:62032970-62032989 Msa1169090:five_prime_UTR 35.0%
! TGAGTTGAGAAGGTTGAATA+AGG + chr7_4:62032980-62032999 Msa1169090:five_prime_UTR 35.0%
AGATATGAATAGGCTCTCTT+CGG + chr7_4:62033162-62033181 Msa1169090:five_prime_UTR 35.0%
TCGGATTAACCAACACAATT+AGG + chr7_4:62033181-62033200 Msa1169090:five_prime_UTR 35.0%
!! TTTTGATTGATGCCGTGTTT+TGG + chr7_4:62033214-62033233 Msa1169090:five_prime_UTR 35.0%
! ATGTTTCTAAAAAGGGTCGA+TGG + chr7_4:62033255-62033274 Msa1169090:five_prime_UTR 35.0%
TGTATGCTATCTTACTGAGT+TGG + chr7_4:62033289-62033308 Msa1169090:five_prime_UTR 35.0%
! TTACTGAGTTGGACTTAAAG+TGG + chr7_4:62033300-62033319 Msa1169090:five_prime_UTR 35.0%
!! TAACTTGCTGAGAAATACCA+TGG + chr7_4:62033330-62033349 Msa1169090:exon 35.0%
TTTGGACAGAGTAAACTTCA+AGG - chr7_4:62033407-62033426 None:intergenic 35.0%
AACCCGCATTTCTAACTTAA+TGG - chr7_4:62033562-62033581 None:intergenic 35.0%
!! CAGTGTGCATATTGATTTAG+AGG + chr7_4:62033802-62033821 Msa1169090:CDS 35.0%
AACCAACACAATTAGGCTCT+TGG + chr7_4:62033188-62033207 Msa1169090:five_prime_UTR 40.0%
TCATACTGATAGGACAGTGA+TGG + chr7_4:62033366-62033385 Msa1169090:CDS 40.0%
CAGTAGAGGATAGGCAAATT+GGG - chr7_4:62033468-62033487 None:intergenic 40.0%
AACCAACACAATTAGGCTCT+TGG + chr7_4:62033188-62033207 Msa1169090:five_prime_UTR 40.0%
TCATACTGATAGGACAGTGA+TGG + chr7_4:62033366-62033385 Msa1169090:CDS 40.0%
CAGTAGAGGATAGGCAAATT+GGG - chr7_4:62033468-62033487 None:intergenic 40.0%
AGGAGCTGCATATGTAGAAG+AGG + chr7_4:62033000-62033019 Msa1169090:five_prime_UTR 45.0%
AGAAGAGGAACCTCACTTGT+AGG + chr7_4:62033015-62033034 Msa1169090:five_prime_UTR 45.0%
TAGAAGAGAGCCTACAAGTG+AGG - chr7_4:62033028-62033047 None:intergenic 45.0%
CACTTGTAGGCTCTCTTCTA+TGG + chr7_4:62033028-62033047 Msa1169090:five_prime_UTR 45.0%
AGCCAAGAGCCTAATTGTGT+TGG - chr7_4:62033193-62033212 None:intergenic 45.0%
TAGTTGTGCCTGCCAAAACA+CGG - chr7_4:62033229-62033248 None:intergenic 45.0%
GCAGTAGAGGATAGGCAAAT+TGG - chr7_4:62033469-62033488 None:intergenic 45.0%
ATTTGCCTATCCTCTACTGC+AGG + chr7_4:62033469-62033488 Msa1169090:intron 45.0%
!! TGCAGGTCAAGGCTGATTTT+GGG + chr7_4:62033486-62033505 Msa1169090:intron 45.0%
GTCCAAGTATTTGCAGAGCA+AGG + chr7_4:62033526-62033545 Msa1169090:CDS 45.0%
AGCCTTGCTCTGCAAATACT+TGG - chr7_4:62033531-62033550 None:intergenic 45.0%
AGCAGAACATGCAATGAGTG+CGG + chr7_4:62033736-62033755 Msa1169090:CDS 45.0%
! CGTGCTATTATGGGTAGAGT+AGG + chr7_4:62033862-62033881 Msa1169090:CDS 45.0%
AGGAGCTGCATATGTAGAAG+AGG + chr7_4:62033000-62033019 Msa1169090:five_prime_UTR 45.0%
AGAAGAGGAACCTCACTTGT+AGG + chr7_4:62033015-62033034 Msa1169090:five_prime_UTR 45.0%
TAGAAGAGAGCCTACAAGTG+AGG - chr7_4:62033028-62033047 None:intergenic 45.0%
CACTTGTAGGCTCTCTTCTA+TGG + chr7_4:62033028-62033047 Msa1169090:five_prime_UTR 45.0%
AGCCAAGAGCCTAATTGTGT+TGG - chr7_4:62033193-62033212 None:intergenic 45.0%
TAGTTGTGCCTGCCAAAACA+CGG - chr7_4:62033229-62033248 None:intergenic 45.0%
GCAGTAGAGGATAGGCAAAT+TGG - chr7_4:62033469-62033488 None:intergenic 45.0%
ATTTGCCTATCCTCTACTGC+AGG + chr7_4:62033469-62033488 Msa1169090:intron 45.0%
!! TGCAGGTCAAGGCTGATTTT+GGG + chr7_4:62033486-62033505 Msa1169090:intron 45.0%
GTCCAAGTATTTGCAGAGCA+AGG + chr7_4:62033526-62033545 Msa1169090:CDS 45.0%
AGCCTTGCTCTGCAAATACT+TGG - chr7_4:62033531-62033550 None:intergenic 45.0%
AGCAGAACATGCAATGAGTG+CGG + chr7_4:62033736-62033755 Msa1169090:CDS 45.0%
!!! GATTGATGCCGTGTTTTGGC+AGG + chr7_4:62033218-62033237 Msa1169090:five_prime_UTR 50.0%
!! GTTTTGGCAGGCACAACTAC+TGG + chr7_4:62033230-62033249 Msa1169090:five_prime_UTR 50.0%
CTTGACCTGCAGTAGAGGAT+AGG - chr7_4:62033477-62033496 None:intergenic 50.0%
CTATCCTCTACTGCAGGTCA+AGG + chr7_4:62033475-62033494 Msa1169090:intron 50.0%
!! CTGCAGGTCAAGGCTGATTT+TGG + chr7_4:62033485-62033504 Msa1169090:intron 50.0%
!!! GATTGATGCCGTGTTTTGGC+AGG + chr7_4:62033218-62033237 Msa1169090:five_prime_UTR 50.0%
!! GTTTTGGCAGGCACAACTAC+TGG + chr7_4:62033230-62033249 Msa1169090:five_prime_UTR 50.0%
CTTGACCTGCAGTAGAGGAT+AGG - chr7_4:62033477-62033496 None:intergenic 50.0%
CTATCCTCTACTGCAGGTCA+AGG + chr7_4:62033475-62033494 Msa1169090:intron 50.0%
!! CTGCAGGTCAAGGCTGATTT+TGG + chr7_4:62033485-62033504 Msa1169090:intron 50.0%
TCAGCCTTGACCTGCAGTAG+AGG - chr7_4:62033482-62033501 None:intergenic 55.0%
TCAGCCTTGACCTGCAGTAG+AGG - chr7_4:62033482-62033501 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr7_4 gene 62032927 62033883 62032927 ID=Msa1169090;Name=Msa1169090
chr7_4 mRNA 62032927 62033883 62032927 ID=Msa1169090-mRNA-1;Parent=Msa1169090;Name=Msa1169090-mRNA-1;_AED=0.44;_eAED=0.44;_QI=422|0.5|0.33|1|0.5|0.66|3|0|112
chr7_4 exon 62032927 62033387 62032927 ID=Msa1169090-mRNA-1:exon:19592;Parent=Msa1169090-mRNA-1
chr7_4 exon 62033491 62033555 62033491 ID=Msa1169090-mRNA-1:exon:19593;Parent=Msa1169090-mRNA-1
chr7_4 exon 62033649 62033883 62033649 ID=Msa1169090-mRNA-1:exon:19594;Parent=Msa1169090-mRNA-1
chr7_4 five_prime_UTR 62032927 62033348 62032927 ID=Msa1169090-mRNA-1:five_prime_utr;Parent=Msa1169090-mRNA-1
chr7_4 CDS 62033349 62033387 62033349 ID=Msa1169090-mRNA-1:cds;Parent=Msa1169090-mRNA-1
chr7_4 CDS 62033491 62033555 62033491 ID=Msa1169090-mRNA-1:cds;Parent=Msa1169090-mRNA-1
chr7_4 CDS 62033649 62033883 62033649 ID=Msa1169090-mRNA-1:cds;Parent=Msa1169090-mRNA-1
Gene Sequence

>Msa1169090

ATGGTTATTAACTTAAATCATACTGATAGGACAGTGATGGTCAAGGCTGATTTTGGGTCTGTTATTGATGCTTTGTCCAAGTATTTGCAGAGCAAGGCTGCCAATCTTCTAATTTTCTTTAACTTTGTTGATTCTAATGACATTCTGTTCATTATTGCTGTTTTCTTTTGTTTTGAAGTCAATGAAATTGGAGCAGAACATGCAATGAGTGCGGACAATGTCACGAGCAAAGAAGTAAATGTAAAGGAAGAAGGTTTCAGTGTGCATATTGATTTAGAGGAACCATGTTTTGCAACAAAGAAAAAAGTACAATTACTCGTGCTATTATGGGTAGAGTAG

Protein sequence

>Msa1169090

MVINLNHTDRTVMVKADFGSVIDALSKYLQSKAANLLIFFNFVDSNDILFIIAVFFCFEVNEIGAEHAMSADNVTSKEVNVKEEGFSVHIDLEEPCFATKKKVQLLVLLWVE*