AlfalfaGEDB | Msa1323190

M. sativa cultivar zhongmu-4 / Msa1323190

KEGG: -
GO: -
PFAM: PF12049.11

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1323190	G7L8J3	95.912	318	13	0	1	318	1	318	0.0	629

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0001950	Msa1323190	0.811605	6.439153e-51	-8.615850e-47
Msa0008340	Msa1323190	0.803078	4.139092e-49	-8.615850e-47
Msa0011720	Msa1323190	0.807613	4.640382e-50	-8.615850e-47
Msa0014920	Msa1323190	0.811619	6.394932e-51	-8.615850e-47
Msa0017510	Msa1323190	0.807731	4.381177e-50	-8.615850e-47
Msa0020630	Msa1323190	0.833615	4.816166e-56	-8.615850e-47
Msa0024680	Msa1323190	0.827225	1.759444e-54	-8.615850e-47
Msa0027540	Msa1323190	0.856792	2.531452e-62	-8.615850e-47
Msa0028060	Msa1323190	0.822736	2.019959e-53	-8.615850e-47
Msa0029540	Msa1323190	-0.819913	9.047377e-53	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1323190	MtrunA17_Chr8g0355941	95.912	318	13	0	1	318	1	318	0.0	629
Msa1323190	MtrunA17_Chr6g0448911	42.188	192	111	0	127	318	110	301	5.80e-54	178

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1323190	AT4G29400.1	73.558	208	54	1	112	318	95	302	2.62e-112	328
Msa1323190	AT5G08400.2	36.071	280	173	2	45	318	21	300	6.98e-55	181
Msa1323190	AT5G08400.1	43.229	192	109	0	127	318	142	333	6.00e-54	179

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Find 69 sgRNAs with CRISPR-Local

Find 518 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGGTTTGGAGCATGTAA+TGG	0.511789	8_4:-31386797	None:intergenic
AAAAGTGTATTGTTGTGTCT+CGG	0.551151	8_4:-31386719	None:intergenic
AAAATACTCCACACAAAGAC+TGG	0.415066	8_4:+31387785	Msa1323190:CDS
AAGAAGGAACAGGTGTAAAA+GGG	0.521054	8_4:-31386768	None:intergenic
AAGACAACTTAGCTCGAATA+TGG	0.395194	8_4:+31392626	Msa1323190:CDS
AAGTGAAAATTCGAACAGTT+TGG	0.419153	8_4:+31386918	Msa1323190:CDS
AATTGTTTCAATACAAATAC+TGG	0.282259	8_4:-31393243	None:intergenic
ACAACTTACTTGCATGTTCA+TGG	0.330028	8_4:-31392389	None:intergenic
ACATCAGAGGATTCAGGTTA+TGG	0.330697	8_4:+31393010	Msa1323190:CDS
ACCAAGTTACGATTATATTC+AGG	0.316714	8_4:+31392552	Msa1323190:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ATAATGAATGTTGCAGAAGT+GGG	-	chr8_4:31385958-31385977	None:intergenic	30.0%
CATGTAATGGTAATACAAGA+AGG	-	chr8_4:31386787-31386806	None:intergenic	30.0%
ATTGTTACGGAAAAAAGGTT+TGG	-	chr8_4:31386812-31386831	None:intergenic	30.0%
AAGTGATTGTTACGGAAAAA+AGG	-	chr8_4:31386817-31386836	None:intergenic	30.0%
AAGTGAAAATTCGAACAGTT+TGG	+	chr8_4:31386918-31386937	Msa1323190:CDS	30.0%
CTTTGATATAGACATCAATG+AGG	+	chr8_4:31386942-31386961	Msa1323190:CDS	30.0%
TTGAGAAAGATGATTATGGT+GGG	+	chr8_4:31386977-31386996	Msa1323190:CDS	30.0%
ATCAACCAGAAAAACATTTC+CGG	-	chr8_4:31387251-31387270	None:intergenic	30.0%
AAACATCACCCTTAAAACAA+TGG	-	chr8_4:31387636-31387655	None:intergenic	30.0%
AGGCTCAATAGATTTAATCT+TGG	-	chr8_4:31387722-31387741	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8_4	gene	31385760	31393259	31385760	ID=Msa1323190;Name=Msa1323190
chr8_4	mRNA	31385760	31393259	31385760	ID=Msa1323190-mRNA-1;Parent=Msa1323190;Name=Msa1323190-mRNA-1;_AED=0.13;_eAED=0.13;_QI=144\|1\|1\|1\|0.57\|0.62\|8\|211\|318
chr8_4	exon	31385760	31385825	31385760	ID=Msa1323190-mRNA-1:exon:10212;Parent=Msa1323190-mRNA-1
chr8_4	exon	31386676	31387188	31386676	ID=Msa1323190-mRNA-1:exon:10213;Parent=Msa1323190-mRNA-1
chr8_4	exon	31387755	31387840	31387755	ID=Msa1323190-mRNA-1:exon:10214;Parent=Msa1323190-mRNA-1
chr8_4	exon	31392167	31392245	31392167	ID=Msa1323190-mRNA-1:exon:10215;Parent=Msa1323190-mRNA-1
chr8_4	exon	31392343	31392402	31392343	ID=Msa1323190-mRNA-1:exon:10216;Parent=Msa1323190-mRNA-1
chr8_4	exon	31392526	31392647	31392526	ID=Msa1323190-mRNA-1:exon:10217;Parent=Msa1323190-mRNA-1
chr8_4	exon	31392754	31392825	31392754	ID=Msa1323190-mRNA-1:exon:10218;Parent=Msa1323190-mRNA-1
chr8_4	exon	31392946	31393259	31392946	ID=Msa1323190-mRNA-1:exon:10219;Parent=Msa1323190-mRNA-1
chr8_4	five_prime_UTR	31385760	31385825	31385760	ID=Msa1323190-mRNA-1:five_prime_utr;Parent=Msa1323190-mRNA-1
chr8_4	five_prime_UTR	31386676	31386753	31386676	ID=Msa1323190-mRNA-1:five_prime_utr;Parent=Msa1323190-mRNA-1
chr8_4	CDS	31386754	31387188	31386754	ID=Msa1323190-mRNA-1:cds;Parent=Msa1323190-mRNA-1
chr8_4	CDS	31387755	31387840	31387755	ID=Msa1323190-mRNA-1:cds;Parent=Msa1323190-mRNA-1
chr8_4	CDS	31392167	31392245	31392167	ID=Msa1323190-mRNA-1:cds;Parent=Msa1323190-mRNA-1
chr8_4	CDS	31392343	31392402	31392343	ID=Msa1323190-mRNA-1:cds;Parent=Msa1323190-mRNA-1
chr8_4	CDS	31392526	31392647	31392526	ID=Msa1323190-mRNA-1:cds;Parent=Msa1323190-mRNA-1
chr8_4	CDS	31392754	31392825	31392754	ID=Msa1323190-mRNA-1:cds;Parent=Msa1323190-mRNA-1
chr8_4	CDS	31392946	31393048	31392946	ID=Msa1323190-mRNA-1:cds;Parent=Msa1323190-mRNA-1
chr8_4	three_prime_UTR	31393049	31393259	31393049	ID=Msa1323190-mRNA-1:three_prime_utr;Parent=Msa1323190-mRNA-1

Gene Sequence

>Msa1323190

ATGATTATATCACACCCTTTTACACCTGTTCCTTCTTGTATTACCATTACATGCTCCAAACCTTTTTTCCGTAACAATCACTTTATCAAATCCTATATACCTCCTTTTCTGCTCTTGAGAAATGACAGAACATCAACATTATTATGTCATGCTAGAAGTGAACGAAGTGAAAATTCGAACAGTTTGGACTTTGATATAGACATCAATGAGGAAGATCAAATACTTGAGAAAGATGATTATGGTGGGTTTTCAGCTCAAGGGATGGAAACAAATGATATTATTGATGTAGATGAAGGACAAGATGATGGTGATGGCAGAAGATATTCAGGTGGAGGACCGTATAAAGGCGGGGATGAGAAGGATTTTGATAGAAATCCAGAATTTGCTGAAATACTTGGTGGTTATCTAGATGATCCGGAAAAGGCACAGTCCAGAATGGAAGAGAGACTAAGAAAAAACAGGAACAAAATACTCCACACAAAGACTGGATCAGGAGTACCAATGAAAGTGTCATTTAATAAATTTGATTTTTCAAACTCATATATATGGTTTGAATTTTACAATGCTCCATTGGCAAAAGACATCATCTTAATTTGTGATGCTATTCGAGCATGGCACATTGTAGGACGACTTGGAGGATGCAATGCCATGAACATGCAACTGTCACAGTCTGAGATAGAAAAACGACCAAGTTACGATTATATTCAGGGAGCGAATGTAACACCAACTACATTTTACAACATTGGGGATCTTGAGGTTCAAGACAACTTAGCTCGAATATGGATAGATATTGGGACTAATGAACCATTGATTCTAGATGTTTTGATAAATGCATTGACACAGATAAGTTCCGACTTTGTTGGGATCAAGCAAGTAGTGTTTGGTGGAGAAGAATTTGAGAATTGGAAAGAGGATTTAACATCAGAGGATTCAGGTTATGGTGTTCATAAGATCTAG

Protein sequence

>Msa1323190

MIISHPFTPVPSCITITCSKPFFRNNHFIKSYIPPFLLLRNDRTSTLLCHARSERSENSNSLDFDIDINEEDQILEKDDYGGFSAQGMETNDIIDVDEGQDDGDGRRYSGGGPYKGGDEKDFDRNPEFAEILGGYLDDPEKAQSRMEERLRKNRNKILHTKTGSGVPMKVSFNKFDFSNSYIWFEFYNAPLAKDIILICDAIRAWHIVGRLGGCNAMNMQLSQSEIEKRPSYDYIQGANVTPTTFYNIGDLEVQDNLARIWIDIGTNEPLILDVLINALTQISSDFVGIKQVVFGGEEFENWKEDLTSEDSGYGVHKI*