AlfalfaGEDB | Msa0054640

M. sativa cultivar zhongmu-4 / Msa0054640

KEGG: K00262
GO: GO:0003674 GO:0003824 GO:0004353 GO:0004354 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006082 GO:0006520 GO:0006536 GO:0006537 GO:0006807 GO:0008150 GO:0008152 GO:0008652 GO:0009058 GO:0009064 GO:0009084 GO:0009987 GO:0016053 GO:0016491 GO:0016638 GO:0016639 GO:0019752 GO:0043436 GO:0043648 GO:0043650 GO:0044237 GO:0044238 GO:0044249 GO:0044281 GO:0044283 GO:0044424 GO:0044464 GO:0046394 GO:0055114 GO:0071704 GO:1901564 GO:1901566 GO:1901576 GO:1901605 GO:1901607
PFAM: -

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0054640	KEH40087.1	73.333	120	22	1	1	120	94	203	1.07e-54	179

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0054640	A0A072VE42	73.333	120	22	1	1	120	94	203	5.13e-55	179

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0006700	Msa0054640	0.813030	3.145353e-51	-8.615850e-47
Msa0020630	Msa0054640	0.817955	2.520385e-52	-8.615850e-47
Msa0027540	Msa0054640	0.826048	3.359384e-54	-8.615850e-47
Msa0030730	Msa0054640	0.812618	3.872131e-51	-8.615850e-47
Msa0032120	Msa0054640	0.816400	5.638406e-52	-8.615850e-47
Msa0032400	Msa0054640	0.804542	2.055173e-49	-8.615850e-47
Msa0037820	Msa0054640	0.806800	6.899514e-50	-8.615850e-47
Msa0038350	Msa0054640	-0.817359	3.435839e-52	-8.615850e-47
Msa0048020	Msa0054640	0.804328	2.277663e-49	-8.615850e-47
Msa0050430	Msa0054640	0.801520	8.663304e-49	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0054640	MtrunA17_Chr8g0341541	64.706	119	32	1	2	120	123	231	6.71e-42	145
Msa0054640	MtrunA17_Chr1g0154541	82.857	70	12	0	1	70	24	93	3.54e-40	129
Msa0054640	MtrunA17_Chr8g0341561	51.087	92	43	1	31	120	1	92	2.44e-19	76.3

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0054640	AT1G51720.4	55.932	118	42	2	3	120	124	231	9.26e-35	125
Msa0054640	AT1G51720.2	55.932	118	42	2	3	120	124	231	2.46e-34	124
Msa0054640	AT1G51720.1	55.932	118	42	2	3	120	124	231	2.68e-34	125
Msa0054640	AT1G51720.3	55.932	118	42	2	3	120	124	231	2.68e-34	125

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Find 176 sgRNAs with CRISPR-Local

Find 386 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATATGCAGAAGAGACAA+AGG	0.596330	1_2:+9334600	Msa0054640:three_prime_UTR
AAAATATTTAAATCTAGATT+CGG	0.324510	1_2:-9332009	None:intergenic
AAAATGGAAGATTTGAAGAA+TGG	0.435371	1_2:-9329121	None:intergenic
AAACATCGGAGAAATCCTAA+CGG	0.661748	1_2:+9330217	Msa0054640:CDS
AAACCGGCTTGTAACTTGTA+AGG	0.473647	1_2:-9331689	None:intergenic
AAACTCTTGAGAGAGTCATC+GGG	0.590180	1_2:+9331309	Msa0054640:CDS
AAAGTAACAAGAACATAAAG+AGG	0.526451	1_2:+9331578	Msa0054640:three_prime_UTR
AAATACAGTTCTAAAACATT+AGG	0.336812	1_2:-9331403	None:intergenic
AAATATTTAAATCTAGATTC+GGG	0.424799	1_2:-9332008	None:intergenic
AAATCCACAGTATGCACGTG+TGG	0.703677	1_2:-9331941	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TGCTTACCCAACAAAAAAAA+AGG	+	chr1_2:9329014-9329033	Msa0054640:five_prime_UTR	30.0%
GATTATTATTTGTGGGTCAT+TGG	+	chr1_2:9329277-9329296	Msa0054640:intron	30.0%
AAAATTATGAGCTTCTGTTG+TGG	+	chr1_2:9329370-9329389	Msa0054640:intron	30.0%
CTCATTAAATATAGGGTTGA+TGG	+	chr1_2:9329428-9329447	Msa0054640:intron	30.0%
TCAAAATAGTAGCATTGTCA+AGG	-	chr1_2:9329504-9329523	None:intergenic	30.0%
ATTGCTTGGCTTAATTTAGT+TGG	-	chr1_2:9329547-9329566	None:intergenic	30.0%
TGAATGATACTTCAGATTGA+TGG	+	chr1_2:9329577-9329596	Msa0054640:intron	30.0%
TGATATGAGTTCTATTCAAC+AGG	+	chr1_2:9329869-9329888	Msa0054640:five_prime_UTR	30.0%
AGATGTCAAAAAGATGCATT+TGG	+	chr1_2:9330583-9330602	Msa0054640:intron	30.0%
AGTGGTCAATAAAAATGGTA+AGG	-	chr1_2:9330760-9330779	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr1_2	gene	9328983	9334709	9328983	ID=Msa0054640;Name=Msa0054640
chr1_2	mRNA	9328983	9334709	9328983	ID=Msa0054640-mRNA-1;Parent=Msa0054640;Name=Msa0054640-mRNA-1;_AED=0.17;_eAED=0.17;_QI=621\|0.5\|0.8\|1\|0.25\|0\|5\|1816\|120
chr1_2	exon	9328983	9329227	9328983	ID=Msa0054640-mRNA-1:exon:3429;Parent=Msa0054640-mRNA-1
chr1_2	exon	9329799	9330373	9329799	ID=Msa0054640-mRNA-1:exon:3430;Parent=Msa0054640-mRNA-1
chr1_2	exon	9330483	9330513	9330483	ID=Msa0054640-mRNA-1:exon:3431;Parent=Msa0054640-mRNA-1
chr1_2	exon	9331214	9332515	9331214	ID=Msa0054640-mRNA-1:exon:3432;Parent=Msa0054640-mRNA-1
chr1_2	exon	9334063	9334709	9334063	ID=Msa0054640-mRNA-1:exon:3433;Parent=Msa0054640-mRNA-1
chr1_2	five_prime_UTR	9328983	9329227	9328983	ID=Msa0054640-mRNA-1:five_prime_utr;Parent=Msa0054640-mRNA-1
chr1_2	five_prime_UTR	9329799	9330174	9329799	ID=Msa0054640-mRNA-1:five_prime_utr;Parent=Msa0054640-mRNA-1
chr1_2	CDS	9330175	9330373	9330175	ID=Msa0054640-mRNA-1:cds;Parent=Msa0054640-mRNA-1
chr1_2	CDS	9330483	9330513	9330483	ID=Msa0054640-mRNA-1:cds;Parent=Msa0054640-mRNA-1
chr1_2	CDS	9331214	9331346	9331214	ID=Msa0054640-mRNA-1:cds;Parent=Msa0054640-mRNA-1
chr1_2	three_prime_UTR	9331347	9332515	9331347	ID=Msa0054640-mRNA-1:three_prime_utr;Parent=Msa0054640-mRNA-1
chr1_2	three_prime_UTR	9334063	9334709	9334063	ID=Msa0054640-mRNA-1:three_prime_utr;Parent=Msa0054640-mRNA-1

Gene Sequence	>Msa0054640 ATGCCAAGGATTTTCTGCTATGTCTGTAGAGAATTTGGCTGGAAACATCGGAGAAATCCTAACGGGAATGAAGGTAGCTGTAATATGCAGATGAATGCCCTCGAAGATCACAATAATAATTTGCTTAATAAAGACGCTTATTGTCTTCAATCGGCCTCCAAATATAAAATTACAGCTGTCAAGCCCGTTAACATAAAAGGTACTGATCTGAGTTGTGGAATACAGAAACTCTGTAATGTCAAAAACAGTGGGATCAATTCTAAAGCTACACTAAAAAGGGATCCTCGTGAGGCCGAGTTCATTCAGGCAGTCCACAAGGTAGTTCAAACTCTTGAGAGAGTCATCGGGAAAAATTCTCAGTGA
Protein sequence	>Msa0054640 MPRIFCYVCREFGWKHRRNPNGNEGSCNMQMNALEDHNNNLLNKDAYCLQSASKYKITAVKPVNIKGTDLSCGIQKLCNVKNSGINSKATLKRDPREAEFIQAVHKVVQTLERVIGKNSQ*