AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1099620

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1099620 MtrunA17_Chr7g0218321 97.959 147 3 0 1 147 176 322 8.92e-105 301
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1099620 AT5G15390.1 78.169 142 31 0 1 142 202 343 5.58e-79 238
Msa1099620 AT5G15390.2 84.746 59 9 0 1 59 202 260 3.82e-33 117

Find 106 sgRNAs with CRISPR-Local

Find 249 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTCCCATGGCTACGAAAAT+TGG 0.172901 7_3:+28331571 None:intergenic
GTTGAAGGGTTGTGTGATTT+TGG 0.242135 7_3:-28334858 Msa1099620:five_prime_UTR
GTTTGCTTGTCACAGGTATA+AGG 0.315275 7_3:-28333119 Msa1099620:intron
CATTTGCTTAGCTAGTTTGC+AGG 0.319793 7_3:+28331711 None:intergenic
TAGTAGTCGGGAACGAAAAT+AGG 0.320600 7_3:-28332766 Msa1099620:intron
CAGAAGCATGGCATATTATA+TGG 0.323726 7_3:+28331630 None:intergenic
TTAGCTAGTTTGCAGGAATA+TGG 0.324172 7_3:+28331718 None:intergenic
TTGGGATAGTAAGCACATTA+AGG 0.351357 7_3:+28331590 None:intergenic
GAAACGAGGAAGGAGAGATT+TGG 0.353406 7_3:-28334921 Msa1099620:five_prime_UTR
CGTCATTTGCAGGGCCGTCA+TGG 0.355451 7_3:-28331945 Msa1099620:intron
GAGAGAGGGTTTGAATTTGA+AGG 0.375465 7_3:+28335208 None:intergenic
GAAATGTTTCGGCGGCGTTT+CGG 0.380970 7_3:-28334836 Msa1099620:five_prime_UTR
ATGGTTAGATATTGAACTTT+GGG 0.381561 7_3:-28333065 Msa1099620:CDS
GTCATTTGCAGGGCCGTCAT+GGG 0.398057 7_3:-28331944 Msa1099620:intron
ACAGGCTTTACATATGATAA+TGG 0.409199 7_3:-28331661 Msa1099620:three_prime_UTR
AATGTTTGTCTTGTTGTTGA+AGG 0.409274 7_3:-28334873 Msa1099620:five_prime_UTR
CAATTAGTAGGTTAACTTCC+TGG 0.413620 7_3:+28331793 None:intergenic
GGGTATTAGTGACGAGGCTT+TGG 0.416031 7_3:-28332438 Msa1099620:CDS
TTCAATGTTTCAGTTGCTGC+AGG 0.418832 7_3:-28332365 Msa1099620:CDS
TTTCGGTCTGCTGATGCTCT+TGG 0.429608 7_3:-28334819 Msa1099620:five_prime_UTR
GAGTCCACCATCCCTTTCAT+TGG 0.432958 7_3:+28332389 None:intergenic
AATGGTTAGATATTGAACTT+TGG 0.437349 7_3:-28333066 Msa1099620:CDS
TGCACCATGCTGTTTGTGAT+AGG 0.439772 7_3:-28332334 Msa1099620:CDS
TTCCCATGGCTACGAAAATT+GGG 0.441657 7_3:+28331572 None:intergenic
CTGAAGTTATTGAAGCTGTT+GGG 0.442622 7_3:-28334956 Msa1099620:five_prime_UTR
TAAGCATGGGTGCGGAGAAA+TGG 0.443836 7_3:-28333084 Msa1099620:exon
TTCGCCTAAGCATGGCGGAC+AGG 0.456095 7_3:-28331679 Msa1099620:three_prime_UTR
TGGATGGTGTCGTTTGGTTG+AGG 0.465669 7_3:+28335116 None:intergenic
ATGTTGTGGATGGTGTCGTT+TGG 0.471323 7_3:+28335110 None:intergenic
AGTTTGCAGGAATATGGATC+AGG 0.471853 7_3:+28331724 None:intergenic
CACTGCAGTATTCCAATGAA+AGG 0.473386 7_3:-28332401 Msa1099620:CDS
GCCTGTCTCATTGTCAATGA+TGG 0.473437 7_3:+28331761 None:intergenic
GACTACTATTGCAGTTGGAC+AGG 0.474059 7_3:+28332781 None:intergenic
ATGTTTGTCTTGTTGTTGAA+GGG 0.487440 7_3:-28334872 Msa1099620:five_prime_UTR
CATCGAAAACTGTCAAGGTA+AGG 0.487532 7_3:+28335005 None:intergenic
GGAACCACCGAGGTTGAGAT+TGG 0.496679 7_3:+28335027 None:intergenic
TCTGAAGTTATTGAAGCTGT+TGG 0.497790 7_3:-28334957 Msa1099620:five_prime_UTR
AGCATCGTGAGGTGGATTTG+TGG 0.501632 7_3:+28335168 None:intergenic
TGAGGAATAGCGGCGGTTTG+TGG 0.501769 7_3:+28335134 None:intergenic
TTCCCGACTACTATTGCAGT+TGG 0.501892 7_3:+28332776 None:intergenic
AAAGCCTGTCCGCCATGCTT+AGG 0.520780 7_3:+28331675 None:intergenic
GTCCAACTGCAATAGTAGTC+GGG 0.524303 7_3:-28332778 Msa1099620:CDS
TGGACGAACATACAATTAGT+AGG 0.525691 7_3:+28331781 None:intergenic
TGAAGAAAGACAAATTCTAC+TGG 0.526302 7_3:-28331910 Msa1099620:CDS
GGACAATCGCCATGTAAGCA+TGG 0.526685 7_3:-28333098 Msa1099620:exon
TCCATCATTGACAATGAGAC+AGG 0.528407 7_3:-28331762 Msa1099620:three_prime_UTR
AGAGGGTTTGAATTTGAAGG+TGG 0.531658 7_3:+28335211 None:intergenic
TTTCGTCTGATGCTAACAAA+AGG 0.536751 7_3:-28334776 Msa1099620:five_prime_UTR
TTTCTATCTATGACATGGAC+TGG 0.537177 7_3:-28332805 Msa1099620:CDS
GAGGGTTGTGACACCTTCGT+TGG 0.539801 7_3:+28335079 None:intergenic
ATGTCAAAATGAATCGAACT+AGG 0.549119 7_3:+28331468 None:intergenic
TTCCTCGTTTCAGAAATACA+TGG 0.556247 7_3:+28334933 None:intergenic
GCGGAGAGAAGAGGAGAGAG+AGG 0.557254 7_3:+28335193 None:intergenic
TGTCCAACTGCAATAGTAGT+CGG 0.560483 7_3:-28332779 Msa1099620:CDS
ATTATTGACTCGTGAGGACA+AGG 0.564220 7_3:-28331501 Msa1099620:three_prime_UTR
GTGAGGTGGATTTGTGGCGG+CGG 0.567255 7_3:+28335174 None:intergenic
TTGTTGCATTCGCCTAAGCA+TGG 0.571624 7_3:-28331687 Msa1099620:three_prime_UTR
CACAAACAGCATGGTGCATG+AGG 0.572207 7_3:+28332339 None:intergenic
AACATCCTCACCTCCAACGA+AGG 0.576742 7_3:-28335092 Msa1099620:five_prime_UTR
GACAATCGCCATGTAAGCAT+GGG 0.576984 7_3:-28333097 Msa1099620:exon
TCGAATTGCAACGACTCATG+TGG 0.580175 7_3:-28332999 Msa1099620:CDS
AGGTGTTTACATTGTTGTTG+AGG 0.582722 7_3:-28334756 Msa1099620:intron
TGACACCTTCGTTGGAGGTG+AGG 0.585666 7_3:+28335087 None:intergenic
ATCGAAAACTGTCAAGGTAA+GGG 0.588501 7_3:+28335006 None:intergenic
ACTGCAGTATTCCAATGAAA+GGG 0.590576 7_3:-28332400 Msa1099620:CDS
CGTTGGAGGTGAGGATGTTG+TGG 0.591361 7_3:+28335096 None:intergenic
CGAATTGCAACGACTCATGT+GGG 0.591975 7_3:-28332998 Msa1099620:CDS
GGTTGTGACACCTTCGTTGG+AGG 0.592186 7_3:+28335082 None:intergenic
AGTAGTTTGTATTATAATCG+AGG 0.595188 7_3:-28331443 Msa1099620:three_prime_UTR
ATGTATTTCTGAAACGAGGA+AGG 0.595398 7_3:-28334931 Msa1099620:five_prime_UTR
TCGCCATGTAAGCATGGGTG+CGG 0.596200 7_3:-28333092 Msa1099620:exon
GGAGGTGAGGATGTTGTGGA+TGG 0.597992 7_3:+28335100 None:intergenic
GTCGTTTGGTTGAGGAATAG+CGG 0.599599 7_3:+28335124 None:intergenic
TCATTTGCAGGGCCGTCATG+GGG 0.599806 7_3:-28331943 Msa1099620:intron
CGAAGATCATGCAAAGCGCA+AGG 0.602561 7_3:-28331844 Msa1099620:CDS
TGAACTTTGGGACTCTACAA+AGG 0.604689 7_3:-28333053 Msa1099620:CDS
TTTGTGGCGGCGGAGAGAAG+AGG 0.607301 7_3:+28335184 None:intergenic
TTCATCACCAATCTCAACCT+CGG 0.607523 7_3:-28335034 Msa1099620:five_prime_UTR
TTGCAGGGGTATTAGTGACG+AGG 0.607935 7_3:-28332444 Msa1099620:intron
TTTGGTATTATTGACTCGTG+AGG 0.608550 7_3:-28331507 Msa1099620:three_prime_UTR
TATTCCAATGAAAGGGATGG+TGG 0.609611 7_3:-28332393 Msa1099620:CDS
ATAGGTTTCTATCTATGACA+TGG 0.614220 7_3:-28332810 Msa1099620:intron
GACCTAGCAGCATTCACACC+AGG 0.615987 7_3:-28331811 Msa1099620:exon
GGTTGAGATTGGTGATGAAG+TGG 0.618911 7_3:+28335038 None:intergenic
TTTCGCATCGAAAACTGTCA+AGG 0.621423 7_3:+28335000 None:intergenic
CAGTATTCCAATGAAAGGGA+TGG 0.627284 7_3:-28332396 Msa1099620:CDS
AATAGCGGCGGTTTGTGGTG+TGG 0.634221 7_3:+28335139 None:intergenic
TTCCTGGTGTGAATGCTGCT+AGG 0.637681 7_3:+28331809 None:intergenic
CGGAGAGAAGAGGAGAGAGA+GGG 0.639831 7_3:+28335194 None:intergenic
GGCCATGTATTTCTGAAACG+AGG 0.642605 7_3:-28334935 Msa1099620:five_prime_UTR
GTTTGCAGGAATATGGATCA+GGG 0.643322 7_3:+28331725 None:intergenic
ATCGTGAGGTGGATTTGTGG+CGG 0.647314 7_3:+28335171 None:intergenic
GAAGGAGAGATTTGGAAAAG+CGG 0.653862 7_3:-28334913 Msa1099620:five_prime_UTR
GGTGTAGCAGCATCGTGAGG+TGG 0.661775 7_3:+28335160 None:intergenic
ATAAGTTGCAACGATTCCCA+TGG 0.663157 7_3:+28331558 None:intergenic
TCGATGCGAAAATGGTTGCG+AGG 0.667934 7_3:-28334988 Msa1099620:five_prime_UTR
AAATCCTATCACAAACAGCA+TGG 0.670020 7_3:+28332330 None:intergenic
CAACTGTCAAATCCCCATGA+CGG 0.678751 7_3:+28331931 None:intergenic
ATCACCAATCTCAACCTCGG+TGG 0.691337 7_3:-28335031 Msa1099620:five_prime_UTR
TTGCATTCGCCTAAGCATGG+CGG 0.702603 7_3:-28331684 Msa1099620:three_prime_UTR
GACTCATGTGGGAATCGATG+CGG 0.703307 7_3:-28332987 Msa1099620:intron
AGAGAGTGATGAACACAACA+TGG 0.703852 7_3:+28335235 None:intergenic
TTTGAAATGTTGAAATCACG+TGG 0.705619 7_3:-28333025 Msa1099620:CDS
TGTGGTGTAGCAGCATCGTG+AGG 0.726344 7_3:+28335157 None:intergenic
GTTTGGTTGAGGAATAGCGG+CGG 0.735664 7_3:+28335127 None:intergenic
TCAAGGTAAGGGAACCACCG+AGG 0.756098 7_3:+28335017 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAATTCATATAAATTCTAA+GGG - chr7_3:28333210-28333229 Msa1099620:intron 10.0%
!! GTAACTTATAATAATTATAA+AGG - chr7_3:28333300-28333319 Msa1099620:intron 10.0%
!!! TTAATTTTATATACTTTATG+TGG - chr7_3:28334409-28334428 Msa1099620:intron 10.0%
!!! ATAGTTCTATAATTTATATT+TGG - chr7_3:28334686-28334705 Msa1099620:intron 10.0%
!!! ATATTGAAATATTGATAATG+TGG - chr7_3:28332738-28332757 Msa1099620:intron 15.0%
!! GATAATTCATATAAATTCTA+AGG - chr7_3:28333209-28333228 Msa1099620:intron 15.0%
!!! TGATTTTGTTTATATTTAAC+CGG - chr7_3:28334219-28334238 Msa1099620:intron 15.0%
!!! GGATTGTTATTATTAAAATA+AGG + chr7_3:28334554-28334573 None:intergenic 15.0%
!!! TGCAATTTTTTTTTTTGTTA+AGG - chr7_3:28335165-28335184 Msa1099620:five_prime_UTR 15.0%
!!! TTTTTTTGTTAAGGTTTTTT+TGG - chr7_3:28335174-28335193 Msa1099620:five_prime_UTR 15.0%
!!! TTCAATTTGTTGTGTATTTT+TGG - chr7_3:28332001-28332020 Msa1099620:intron 20.0%
!!! TGATATTAAGTTGGTAATAT+CGG - chr7_3:28332615-28332634 Msa1099620:intron 20.0%
!! TACGATTTCAATTATTAACT+TGG - chr7_3:28332781-28332800 Msa1099620:CDS 20.0%
!! ACGATTTCAATTATTAACTT+GGG - chr7_3:28332782-28332801 Msa1099620:CDS 20.0%
!! GATAATATAAGAATAGAGTA+GGG + chr7_3:28332989-28333008 None:intergenic 20.0%
!! TGATAATATAAGAATAGAGT+AGG + chr7_3:28332990-28333009 None:intergenic 20.0%
!!! TGAATTATCGATTAAAAGAT+GGG + chr7_3:28333198-28333217 None:intergenic 20.0%
!!! ATGAATTATCGATTAAAAGA+TGG + chr7_3:28333199-28333218 None:intergenic 20.0%
!! ATTATATATTTCCTTAGTGT+TGG - chr7_3:28334066-28334085 Msa1099620:intron 20.0%
!! TCTTAAAGTTCTATACATAT+TGG - chr7_3:28334509-28334528 Msa1099620:intron 20.0%
!! CTTTGAATAACCAAAAAATA+AGG + chr7_3:28334621-28334640 None:intergenic 20.0%
!!! TCTTGTTACTTTGATTTTGA+AGG + chr7_3:28331415-28331434 None:intergenic 25.0%
!! ATTTCTCTTATGGTTTTTCT+AGG + chr7_3:28332197-28332216 None:intergenic 25.0%
! GCAACTAATAATTTCTCTTA+TGG + chr7_3:28332207-28332226 None:intergenic 25.0%
! CTTTATGAACTCATTGAATT+AGG - chr7_3:28332440-28332459 Msa1099620:CDS 25.0%
! AAGTAATTGAATATCCTAAC+TGG - chr7_3:28332470-28332489 Msa1099620:intron 25.0%
! AGACTAGAATGATATTAAGT+TGG - chr7_3:28332606-28332625 Msa1099620:intron 25.0%
!!! ATATTGATAATGTGGAGAAT+TGG - chr7_3:28332746-28332765 Msa1099620:intron 25.0%
!! TTAGTTGCTGTTCATAAAAT+AGG - chr7_3:28332805-28332824 Msa1099620:CDS 25.0%
!! AACAAAATTTTCTACAGTAG+AGG + chr7_3:28332920-28332939 None:intergenic 25.0%
! CTCTATTCTTATATTATCAG+TGG - chr7_3:28332991-28333010 Msa1099620:CDS 25.0%
! TGGAGTTAGTTAAAATTAGT+AGG - chr7_3:28333089-28333108 Msa1099620:exon 25.0%
!! TTAAAATTAGTAGGTTAGTC+AGG - chr7_3:28333098-28333117 Msa1099620:exon 25.0%
! AACATTAGTTAGTGTAATTG+AGG - chr7_3:28333141-28333160 Msa1099620:intron 25.0%
!! TGTTAGCTTAACTTCATTTA+TGG + chr7_3:28333373-28333392 None:intergenic 25.0%
! TATGTAGCATTATTCAAGAA+GGG + chr7_3:28333437-28333456 None:intergenic 25.0%
! TTATGTAGCATTATTCAAGA+AGG + chr7_3:28333438-28333457 None:intergenic 25.0%
! AATGGTTAGATATTGAACTT+TGG - chr7_3:28333633-28333652 Msa1099620:intron 25.0%
! ATGGTTAGATATTGAACTTT+GGG - chr7_3:28333634-28333653 Msa1099620:intron 25.0%
!! TGTTCTATTTCTTGTAACTT+GGG - chr7_3:28334185-28334204 Msa1099620:intron 25.0%
! GAATAACCAAAAAATAAGGT+GGG + chr7_3:28334617-28334636 None:intergenic 25.0%
! TGAATAACCAAAAAATAAGG+TGG + chr7_3:28334618-28334637 None:intergenic 25.0%
!! AGTAGTTTGTATTATAATCG+AGG - chr7_3:28335256-28335275 Msa1099620:five_prime_UTR 25.0%
!!! TGTTACTTTGATTTTGAAGG+TGG + chr7_3:28331412-28331431 None:intergenic 30.0%
! TTTTCGTTGCATTTTCATGA+GGG + chr7_3:28331641-28331660 None:intergenic 30.0%
!! AATGTTTGTCTTGTTGTTGA+AGG - chr7_3:28331826-28331845 Msa1099620:exon 30.0%
!! ATGTTTGTCTTGTTGTTGAA+GGG - chr7_3:28331827-28331846 Msa1099620:CDS 30.0%
!! GTGTGATTTTGGAAATGTTT+CGG - chr7_3:28331852-28331871 Msa1099620:CDS 30.0%
GTTGAGGTGAAATTTGATAA+TGG - chr7_3:28331959-28331978 Msa1099620:intron 30.0%
!!! GTGTATTTTTGGTTTGTAGA+CGG - chr7_3:28332012-28332031 Msa1099620:intron 30.0%
! TTTACCGTCTTTTCTACTAT+AGG + chr7_3:28332069-28332088 None:intergenic 30.0%
AGAGAAATTATTAGTTGCTG+AGG - chr7_3:28332209-28332228 Msa1099620:intron 30.0%
TATAAATTCAAGCACCAGTT+AGG + chr7_3:28332487-28332506 None:intergenic 30.0%
TAACTGGTGCTTGAATTTAT+AGG - chr7_3:28332486-28332505 Msa1099620:intron 30.0%
TGCGTTTGATATTAGATAGA+TGG - chr7_3:28332673-28332692 Msa1099620:intron 30.0%
! TTACAATTCTAGCGACTTTT+TGG - chr7_3:28332714-28332733 Msa1099620:intron 30.0%
GTGATTATTGTTAAGACAGT+TGG - chr7_3:28333069-28333088 Msa1099620:CDS 30.0%
ATGAACTTCATATGAGAAGT+AGG + chr7_3:28333262-28333281 None:intergenic 30.0%
CAATAAAAAGCTCATGAGAT+TGG + chr7_3:28333467-28333486 None:intergenic 30.0%
! TTTGAAATGTTGAAATCACG+TGG - chr7_3:28333674-28333693 Msa1099620:intron 30.0%
TGCATCAATACATGATTACA+GGG + chr7_3:28333791-28333810 None:intergenic 30.0%
ATGCATCAATACATGATTAC+AGG + chr7_3:28333792-28333811 None:intergenic 30.0%
GAAAATCCACATATGTCATA+CGG - chr7_3:28333837-28333856 Msa1099620:intron 30.0%
!!! GACTTTTTCTTGCTGTTTAT+AGG - chr7_3:28333871-28333890 Msa1099620:intron 30.0%
ATAGGTTTCTATCTATGACA+TGG - chr7_3:28333889-28333908 Msa1099620:intron 30.0%
!! GCATCTTCAAAAGTATTACA+TGG + chr7_3:28334124-28334143 None:intergenic 30.0%
! CTGTTCTATTTCTTGTAACT+TGG - chr7_3:28334184-28334203 Msa1099620:intron 30.0%
!!! GTTATTCCCACCTTATTTTT+TGG - chr7_3:28334608-28334627 Msa1099620:intron 30.0%
! TAATTTTCACGTCATTTGCA+GGG - chr7_3:28334745-28334764 Msa1099620:intron 30.0%
TGAAGAAAGACAAATTCTAC+TGG - chr7_3:28334789-28334808 Msa1099620:five_prime_UTR 30.0%
ACAGGCTTTACATATGATAA+TGG - chr7_3:28335038-28335057 Msa1099620:five_prime_UTR 30.0%
!! TTACAGTTTTAACAGAAGCA+TGG + chr7_3:28335084-28335103 None:intergenic 30.0%
ATGTCAAAATGAATCGAACT+AGG + chr7_3:28335234-28335253 None:intergenic 30.0%
! GTTTTCGTTGCATTTTCATG+AGG + chr7_3:28331642-28331661 None:intergenic 35.0%
ATCGAAAACTGTCAAGGTAA+GGG + chr7_3:28331696-28331715 None:intergenic 35.0%
! TCTGAAGTTATTGAAGCTGT+TGG - chr7_3:28331742-28331761 Msa1099620:three_prime_UTR 35.0%
CTGAAGTTATTGAAGCTGTT+GGG - chr7_3:28331743-28331762 Msa1099620:three_prime_UTR 35.0%
TTCCTCGTTTCAGAAATACA+TGG + chr7_3:28331769-28331788 None:intergenic 35.0%
!! ATGTATTTCTGAAACGAGGA+AGG - chr7_3:28331768-28331787 Msa1099620:three_prime_UTR 35.0%
!! TGATTTTGGAAATGTTTCGG+CGG - chr7_3:28331855-28331874 Msa1099620:CDS 35.0%
TTTCGTCTGATGCTAACAAA+AGG - chr7_3:28331923-28331942 Msa1099620:CDS 35.0%
! AGGTGTTTACATTGTTGTTG+AGG - chr7_3:28331943-28331962 Msa1099620:intron 35.0%
GTGACAATATTAGAGAGAGT+TGG - chr7_3:28332036-28332055 Msa1099620:intron 35.0%
AGCACCTATAGTAGAAAAGA+CGG - chr7_3:28332062-28332081 Msa1099620:intron 35.0%
GTAGAAAAGACGGTAAAACT+AGG - chr7_3:28332072-28332091 Msa1099620:intron 35.0%
AAGACGGTAAAACTAGGTTT+AGG - chr7_3:28332078-28332097 Msa1099620:intron 35.0%
AAAACTAGGTTTAGGTGGTT+TGG - chr7_3:28332086-28332105 Msa1099620:intron 35.0%
AAACTAGGTTTAGGTGGTTT+GGG - chr7_3:28332087-28332106 Msa1099620:intron 35.0%
! AAAGCCTTATCCCACTAAAT+GGG + chr7_3:28332279-28332298 None:intergenic 35.0%
! GTTGTTGTTGTTGTTGTAGA+CGG - chr7_3:28332299-28332318 Msa1099620:intron 35.0%
! AACATTTTCCGTGGTTACAT+AGG + chr7_3:28332384-28332403 None:intergenic 35.0%
ATGTAACCACGGAAAATGTT+TGG - chr7_3:28332384-28332403 Msa1099620:CDS 35.0%
AGTCTAGTTGTTCACATCAT+CGG - chr7_3:28332517-28332536 Msa1099620:intron 35.0%
AATGAAATCACCTTAACCTC+AGG - chr7_3:28332851-28332870 Msa1099620:intron 35.0%
ATGAAATCACCTTAACCTCA+GGG - chr7_3:28332852-28332871 Msa1099620:intron 35.0%
! ATTAGTAGGTTAGTCAGGTA+TGG - chr7_3:28333103-28333122 Msa1099620:five_prime_UTR 35.0%
CGATTAAAAGATGGGTAATG+AGG + chr7_3:28333190-28333209 None:intergenic 35.0%
ATATGAGAAGTAGGCTTGAT+AGG + chr7_3:28333253-28333272 None:intergenic 35.0%
ACTTCATTTATGGCTGAGAA+TGG + chr7_3:28333363-28333382 None:intergenic 35.0%
TTTCTATCTATGACATGGAC+TGG - chr7_3:28333894-28333913 Msa1099620:intron 35.0%
AAGACAAGCGACATATATGA+AGG + chr7_3:28333965-28333984 None:intergenic 35.0%
!! TATCAAAAGCACCAACACTA+AGG + chr7_3:28334080-28334099 None:intergenic 35.0%
! ATGTGAGCTTGTTTGTATGT+TGG - chr7_3:28334146-28334165 Msa1099620:intron 35.0%
CTGCAAAAAGAACATAACAC+CGG + chr7_3:28334241-28334260 None:intergenic 35.0%
!! GGTGTTATGTTCTTTTTGCA+GGG - chr7_3:28334240-28334259 Msa1099620:intron 35.0%
! GTGTTATGTTCTTTTTGCAG+GGG - chr7_3:28334241-28334260 Msa1099620:intron 35.0%
ACTGCAGTATTCCAATGAAA+GGG - chr7_3:28334299-28334318 Msa1099620:intron 35.0%
AAATCCTATCACAAACAGCA+TGG + chr7_3:28334372-28334391 None:intergenic 35.0%
ATACACAGCAGTATAGTGAT+TGG + chr7_3:28334575-28334594 None:intergenic 35.0%
AAATAAGGTGGGAATAACCA+TGG + chr7_3:28334606-28334625 None:intergenic 35.0%
! TGGTTTTTCTGCATCTCATT+TGG - chr7_3:28334706-28334725 Msa1099620:intron 35.0%
! GTAATTTTCACGTCATTTGC+AGG - chr7_3:28334744-28334763 Msa1099620:intron 35.0%
CAATTAGTAGGTTAACTTCC+TGG + chr7_3:28334909-28334928 None:intergenic 35.0%
TGGACGAACATACAATTAGT+AGG + chr7_3:28334921-28334940 None:intergenic 35.0%
! TTAGCTAGTTTGCAGGAATA+TGG + chr7_3:28334984-28335003 None:intergenic 35.0%
! CAGAAGCATGGCATATTATA+TGG + chr7_3:28335072-28335091 None:intergenic 35.0%
TTGGGATAGTAAGCACATTA+AGG + chr7_3:28335112-28335131 None:intergenic 35.0%
!! TTTGGTATTATTGACTCGTG+AGG - chr7_3:28335192-28335211 Msa1099620:five_prime_UTR 35.0%
AGAGAGTGATGAACACAACA+TGG + chr7_3:28331467-28331486 None:intergenic 40.0%
AGAGGGTTTGAATTTGAAGG+TGG + chr7_3:28331491-28331510 None:intergenic 40.0%
GAGAGAGGGTTTGAATTTGA+AGG + chr7_3:28331494-28331513 None:intergenic 40.0%
TTCATCACCAATCTCAACCT+CGG - chr7_3:28331665-28331684 Msa1099620:three_prime_UTR 40.0%
CATCGAAAACTGTCAAGGTA+AGG + chr7_3:28331697-28331716 None:intergenic 40.0%
TTTCGCATCGAAAACTGTCA+AGG + chr7_3:28331702-28331721 None:intergenic 40.0%
! GACAGTTTTCGATGCGAAAA+TGG - chr7_3:28331703-28331722 Msa1099620:three_prime_UTR 40.0%
GAAGGAGAGATTTGGAAAAG+CGG - chr7_3:28331786-28331805 Msa1099620:three_prime_UTR 40.0%
! GTTGAAGGGTTGTGTGATTT+TGG - chr7_3:28331841-28331860 Msa1099620:CDS 40.0%
ACGGTAAAACTAGGTTTAGG+TGG - chr7_3:28332081-28332100 Msa1099620:intron 40.0%
! AGCTATGAGAGTGTATCAGA+TGG - chr7_3:28332139-28332158 Msa1099620:intron 40.0%
TGGAGTATAGTCAGATCACT+AGG - chr7_3:28332159-28332178 Msa1099620:intron 40.0%
GGAGTATAGTCAGATCACTA+GGG - chr7_3:28332160-28332179 Msa1099620:intron 40.0%
! AAGCCTTATCCCACTAAATG+GGG + chr7_3:28332278-28332297 None:intergenic 40.0%
! CAAAGCCTTATCCCACTAAA+TGG + chr7_3:28332280-28332299 None:intergenic 40.0%
AAACTCACAGACACACACAA+AGG - chr7_3:28332340-28332359 Msa1099620:CDS 40.0%
AACTCACAGACACACACAAA+GGG - chr7_3:28332341-28332360 Msa1099620:CDS 40.0%
ACACACAAAGGGGATATCTT+GGG - chr7_3:28332352-28332371 Msa1099620:CDS 40.0%
TTCATAAAGCCTAGCTCAAC+TGG + chr7_3:28332429-28332448 None:intergenic 40.0%
AGTCAAATTCGTCCTTCTGT+TGG - chr7_3:28332574-28332593 Msa1099620:intron 40.0%
TCTCTGCAAATACCAACAGA+AGG + chr7_3:28332589-28332608 None:intergenic 40.0%
ATATTCACACCCTGAGGTTA+AGG + chr7_3:28332864-28332883 None:intergenic 40.0%
CATAAGATATTCACACCCTG+AGG + chr7_3:28332870-28332889 None:intergenic 40.0%
CTCAGGGTGTGAATATCTTA+TGG - chr7_3:28332868-28332887 Msa1099620:intron 40.0%
AGGGTGTGAATATCTTATGG+CGG - chr7_3:28332871-28332890 Msa1099620:intron 40.0%
! ACATGAAGCACTAACACTTC+AGG - chr7_3:28332945-28332964 Msa1099620:intron 40.0%
TGTTTGTATCAGTGCAACGT+TGG - chr7_3:28333034-28333053 Msa1099620:CDS 40.0%
! GCAACGTTGGTTATTATTGC+TGG - chr7_3:28333047-28333066 Msa1099620:CDS 40.0%
! TAGGTTAGTCAGGTATGGTT+AGG - chr7_3:28333108-28333127 Msa1099620:intron 40.0%
TCAGGTATGGTTAGGAGAAT+TGG - chr7_3:28333116-28333135 Msa1099620:intron 40.0%
ATGGGTAATGAGGACATGTA+TGG + chr7_3:28333180-28333199 None:intergenic 40.0%
! TAACCAAGTTTGCTTGTCAC+AGG - chr7_3:28333573-28333592 Msa1099620:intron 40.0%
ATACCTGTGACAAGCAAACT+TGG + chr7_3:28333579-28333598 None:intergenic 40.0%
GTTTGCTTGTCACAGGTATA+AGG - chr7_3:28333580-28333599 Msa1099620:intron 40.0%
!! TGAACTTTGGGACTCTACAA+AGG - chr7_3:28333646-28333665 Msa1099620:intron 40.0%
! CAGGGTTCAAACTATTTCTC+TGG + chr7_3:28333773-28333792 None:intergenic 40.0%
TGTCCAACTGCAATAGTAGT+CGG - chr7_3:28333920-28333939 Msa1099620:intron 40.0%
TAGTAGTCGGGAACGAAAAT+AGG - chr7_3:28333933-28333952 Msa1099620:intron 40.0%
!! GTGTTGGTGCTTTTGATATG+CGG - chr7_3:28334082-28334101 Msa1099620:intron 40.0%
!!! CGGTGTTATGTTCTTTTTGC+AGG - chr7_3:28334239-28334258 Msa1099620:intron 40.0%
CACTGCAGTATTCCAATGAA+AGG - chr7_3:28334298-28334317 Msa1099620:intron 40.0%
CAGTATTCCAATGAAAGGGA+TGG - chr7_3:28334303-28334322 Msa1099620:intron 40.0%
TATTCCAATGAAAGGGATGG+TGG - chr7_3:28334306-28334325 Msa1099620:intron 40.0%
TTCAATGTTTCAGTTGCTGC+AGG - chr7_3:28334334-28334353 Msa1099620:intron 40.0%
ACATAGAAACAACGCTTTGC+TGG - chr7_3:28334465-28334484 Msa1099620:intron 40.0%
TCCATCATTGACAATGAGAC+AGG - chr7_3:28334937-28334956 Msa1099620:five_prime_UTR 40.0%
GTTTGCAGGAATATGGATCA+GGG + chr7_3:28334977-28334996 None:intergenic 40.0%
AGTTTGCAGGAATATGGATC+AGG + chr7_3:28334978-28334997 None:intergenic 40.0%
! CATTTGCTTAGCTAGTTTGC+AGG + chr7_3:28334991-28335010 None:intergenic 40.0%
! TATCCCAATTTTCGTAGCCA+TGG - chr7_3:28335124-28335143 Msa1099620:five_prime_UTR 40.0%
! ATCCCAATTTTCGTAGCCAT+GGG - chr7_3:28335125-28335144 Msa1099620:five_prime_UTR 40.0%
TTCCCATGGCTACGAAAATT+GGG + chr7_3:28335130-28335149 None:intergenic 40.0%
ATTCCCATGGCTACGAAAAT+TGG + chr7_3:28335131-28335150 None:intergenic 40.0%
ATAAGTTGCAACGATTCCCA+TGG + chr7_3:28335144-28335163 None:intergenic 40.0%
!! ATTATTGACTCGTGAGGACA+AGG - chr7_3:28335198-28335217 Msa1099620:five_prime_UTR 40.0%
GTCGTTTGGTTGAGGAATAG+CGG + chr7_3:28331578-28331597 None:intergenic 45.0%
!! ATGTTGTGGATGGTGTCGTT+TGG + chr7_3:28331592-28331611 None:intergenic 45.0%
!! GGTTGAGATTGGTGATGAAG+TGG + chr7_3:28331664-28331683 None:intergenic 45.0%
!! GGCCATGTATTTCTGAAACG+AGG - chr7_3:28331764-28331783 Msa1099620:three_prime_UTR 45.0%
GAAACGAGGAAGGAGAGATT+TGG - chr7_3:28331778-28331797 Msa1099620:three_prime_UTR 45.0%
CTTATCCCACTAAATGGGGT+CGG + chr7_3:28332274-28332293 None:intergenic 45.0%
! GTGTGTCTGTGAGTTTTCAG+CGG + chr7_3:28332336-28332355 None:intergenic 45.0%
ACTCACAGACACACACAAAG+GGG - chr7_3:28332342-28332361 Msa1099620:CDS 45.0%
CACACACAAAGGGGATATCT+TGG - chr7_3:28332351-28332370 Msa1099620:CDS 45.0%
GGTTTGAGCCTATGTAACCA+CGG - chr7_3:28332373-28332392 Msa1099620:CDS 45.0%
! GTTAGGCCAAACATTTTCCG+TGG + chr7_3:28332393-28332412 None:intergenic 45.0%
CTGGCAATGCTGAGATTGTT+AGG + chr7_3:28332410-28332429 None:intergenic 45.0%
!! GCACTAACACTTCAGGTTGA+AGG - chr7_3:28332952-28332971 Msa1099620:intron 45.0%
GACAATCGCCATGTAAGCAT+GGG - chr7_3:28333602-28333621 Msa1099620:intron 45.0%
TCGAATTGCAACGACTCATG+TGG - chr7_3:28333700-28333719 Msa1099620:intron 45.0%
CGAATTGCAACGACTCATGT+GGG - chr7_3:28333701-28333720 Msa1099620:intron 45.0%
GCTTCACCGTATGACATATG+TGG + chr7_3:28333846-28333865 None:intergenic 45.0%
GACTACTATTGCAGTTGGAC+AGG + chr7_3:28333921-28333940 None:intergenic 45.0%
GTCCAACTGCAATAGTAGTC+GGG - chr7_3:28333921-28333940 Msa1099620:intron 45.0%
TTCCCGACTACTATTGCAGT+TGG + chr7_3:28333926-28333945 None:intergenic 45.0%
! TGGTGCTTTTGATATGCGGT+TGG - chr7_3:28334086-28334105 Msa1099620:intron 45.0%
! TGCACCATGCTGTTTGTGAT+AGG - chr7_3:28334365-28334384 Msa1099620:intron 45.0%
GCTGTGTATTCTTTCCTCCA+TGG - chr7_3:28334586-28334605 Msa1099620:intron 45.0%
TAAGGTGGGAATAACCATGG+AGG + chr7_3:28334603-28334622 None:intergenic 45.0%
CAACTGTCAAATCCCCATGA+CGG + chr7_3:28334771-28334790 None:intergenic 45.0%
GCCTGTCTCATTGTCAATGA+TGG + chr7_3:28334941-28334960 None:intergenic 45.0%
TTGTTGCATTCGCCTAAGCA+TGG - chr7_3:28335012-28335031 Msa1099620:five_prime_UTR 45.0%
!! TAACTTATAATAATTATAAA+GGG - chr7_3:28333301-28333320 Msa1099620:intron 5.0%
! ATCGTGAGGTGGATTTGTGG+CGG + chr7_3:28331531-28331550 None:intergenic 50.0%
! AGCATCGTGAGGTGGATTTG+TGG + chr7_3:28331534-28331553 None:intergenic 50.0%
GTTTGGTTGAGGAATAGCGG+CGG + chr7_3:28331575-28331594 None:intergenic 50.0%
!! TGGATGGTGTCGTTTGGTTG+AGG + chr7_3:28331586-28331605 None:intergenic 50.0%
AACATCCTCACCTCCAACGA+AGG - chr7_3:28331607-28331626 Msa1099620:three_prime_UTR 50.0%
ATCACCAATCTCAACCTCGG+TGG - chr7_3:28331668-28331687 Msa1099620:three_prime_UTR 50.0%
TCGATGCGAAAATGGTTGCG+AGG - chr7_3:28331711-28331730 Msa1099620:three_prime_UTR 50.0%
GAAATGTTTCGGCGGCGTTT+CGG - chr7_3:28331863-28331882 Msa1099620:CDS 50.0%
TTTCGGTCTGCTGATGCTCT+TGG - chr7_3:28331880-28331899 Msa1099620:CDS 50.0%
GTTGCTGAGGAGTTGCATTG+AGG - chr7_3:28332222-28332241 Msa1099620:intron 50.0%
ATGAAGCCGACCCCATTTAG+TGG - chr7_3:28332265-28332284 Msa1099620:intron 50.0%
TGAAGCCGACCCCATTTAGT+GGG - chr7_3:28332266-28332285 Msa1099620:intron 50.0%
CGACCCCATTTAGTGGGATA+AGG - chr7_3:28332272-28332291 Msa1099620:intron 50.0%
TCAGCATTGCCAGTTGAGCT+AGG - chr7_3:28332417-28332436 Msa1099620:CDS 50.0%
!! CTTCAGGTTGAAGGCATGTC+TGG - chr7_3:28332961-28332980 Msa1099620:intron 50.0%
GGACAATCGCCATGTAAGCA+TGG - chr7_3:28333601-28333620 Msa1099620:intron 50.0%
TAAGCATGGGTGCGGAGAAA+TGG - chr7_3:28333615-28333634 Msa1099620:intron 50.0%
GACTCATGTGGGAATCGATG+CGG - chr7_3:28333712-28333731 Msa1099620:intron 50.0%
!! TTGCAGGGGTATTAGTGACG+AGG - chr7_3:28334255-28334274 Msa1099620:intron 50.0%
GGGTATTAGTGACGAGGCTT+TGG - chr7_3:28334261-28334280 Msa1099620:intron 50.0%
GAGTCCACCATCCCTTTCAT+TGG + chr7_3:28334313-28334332 None:intergenic 50.0%
CACAAACAGCATGGTGCATG+AGG + chr7_3:28334363-28334382 None:intergenic 50.0%
CGAAGATCATGCAAAGCGCA+AGG - chr7_3:28334855-28334874 Msa1099620:five_prime_UTR 50.0%
TTCCTGGTGTGAATGCTGCT+AGG + chr7_3:28334893-28334912 None:intergenic 50.0%
TTGCATTCGCCTAAGCATGG+CGG - chr7_3:28335015-28335034 Msa1099620:five_prime_UTR 50.0%
CGGAGAGAAGAGGAGAGAGA+GGG + chr7_3:28331508-28331527 None:intergenic 55.0%
TGTGGTGTAGCAGCATCGTG+AGG + chr7_3:28331545-28331564 None:intergenic 55.0%
AATAGCGGCGGTTTGTGGTG+TGG + chr7_3:28331563-28331582 None:intergenic 55.0%
TGAGGAATAGCGGCGGTTTG+TGG + chr7_3:28331568-28331587 None:intergenic 55.0%
GGAGGTGAGGATGTTGTGGA+TGG + chr7_3:28331602-28331621 None:intergenic 55.0%
CGTTGGAGGTGAGGATGTTG+TGG + chr7_3:28331606-28331625 None:intergenic 55.0%
TGACACCTTCGTTGGAGGTG+AGG + chr7_3:28331615-28331634 None:intergenic 55.0%
GGTTGTGACACCTTCGTTGG+AGG + chr7_3:28331620-28331639 None:intergenic 55.0%
GAGGGTTGTGACACCTTCGT+TGG + chr7_3:28331623-28331642 None:intergenic 55.0%
GGAACCACCGAGGTTGAGAT+TGG + chr7_3:28331675-28331694 None:intergenic 55.0%
TCAAGGTAAGGGAACCACCG+AGG + chr7_3:28331685-28331704 None:intergenic 55.0%
TCGCCATGTAAGCATGGGTG+CGG - chr7_3:28333607-28333626 Msa1099620:intron 55.0%
TCTCCGCACCCATGCTTACA+TGG + chr7_3:28333613-28333632 None:intergenic 55.0%
GTCATTTGCAGGGCCGTCAT+GGG - chr7_3:28334755-28334774 Msa1099620:intron 55.0%
TCATTTGCAGGGCCGTCATG+GGG - chr7_3:28334756-28334775 Msa1099620:intron 55.0%
GACCTAGCAGCATTCACACC+AGG - chr7_3:28334888-28334907 Msa1099620:five_prime_UTR 55.0%
AAAGCCTGTCCGCCATGCTT+AGG + chr7_3:28335027-28335046 None:intergenic 55.0%
GCGGAGAGAAGAGGAGAGAG+AGG + chr7_3:28331509-28331528 None:intergenic 60.0%
TTTGTGGCGGCGGAGAGAAG+AGG + chr7_3:28331518-28331537 None:intergenic 60.0%
! GTGAGGTGGATTTGTGGCGG+CGG + chr7_3:28331528-28331547 None:intergenic 60.0%
GGTGTAGCAGCATCGTGAGG+TGG + chr7_3:28331542-28331561 None:intergenic 60.0%
CGTCATTTGCAGGGCCGTCA+TGG - chr7_3:28334754-28334773 Msa1099620:intron 60.0%
!! TTCGCCTAAGCATGGCGGAC+AGG - chr7_3:28335020-28335039 Msa1099620:five_prime_UTR 60.0%
Chromosome Type Strat End Strand Name
chr7_3 gene 28331403 28335318 28331403 ID=Msa1099620;Name=Msa1099620
chr7_3 mRNA 28331403 28335318 28331403 ID=Msa1099620-mRNA-1;Parent=Msa1099620;Name=Msa1099620-mRNA-1;_AED=0.06;_eAED=0.06;_QI=587|1|1|1|0.75|0.8|5|424|147
chr7_3 exon 28331403 28331955 28331403 ID=Msa1099620-mRNA-1:exon:5984;Parent=Msa1099620-mRNA-1
chr7_3 exon 28332322 28332460 28332322 ID=Msa1099620-mRNA-1:exon:5983;Parent=Msa1099620-mRNA-1
chr7_3 exon 28332767 28332828 28332767 ID=Msa1099620-mRNA-1:exon:5982;Parent=Msa1099620-mRNA-1
chr7_3 exon 28332988 28333126 28332988 ID=Msa1099620-mRNA-1:exon:5981;Parent=Msa1099620-mRNA-1
chr7_3 exon 28334757 28335318 28334757 ID=Msa1099620-mRNA-1:exon:5980;Parent=Msa1099620-mRNA-1
chr7_3 five_prime_UTR 28334757 28335318 28334757 ID=Msa1099620-mRNA-1:five_prime_utr;Parent=Msa1099620-mRNA-1
chr7_3 five_prime_UTR 28333102 28333126 28333102 ID=Msa1099620-mRNA-1:five_prime_utr;Parent=Msa1099620-mRNA-1
chr7_3 CDS 28332988 28333101 28332988 ID=Msa1099620-mRNA-1:cds;Parent=Msa1099620-mRNA-1
chr7_3 CDS 28332767 28332828 28332767 ID=Msa1099620-mRNA-1:cds;Parent=Msa1099620-mRNA-1
chr7_3 CDS 28332322 28332460 28332322 ID=Msa1099620-mRNA-1:cds;Parent=Msa1099620-mRNA-1
chr7_3 CDS 28331827 28331955 28331827 ID=Msa1099620-mRNA-1:cds;Parent=Msa1099620-mRNA-1
chr7_3 three_prime_UTR 28331403 28331826 28331403 ID=Msa1099620-mRNA-1:three_prime_utr;Parent=Msa1099620-mRNA-1
Gene Sequence

>Msa1099620

ATGGGTGCGGAGAAATGGTTAGATATTGAACTTTGGGACTCTACAAAGGAGTGCTTTGAAATGTTGAAATCACGTGGTTATCGAATTGCAACGACTCATGTGGGAATCGATGCGGTTTCTATCTATGACATGGACTGGTCCTGTCCAACTGCAATAGTAGTCGGGAACGAAAATAGGGGTATTAGTGACGAGGCTTTGGCTATGTCAGATTTGCACTGCAGTATTCCAATGAAAGGGATGGTGGACTCTTTCAATGTTTCAGTTGCTGCAGGAATCCTCATGCACCATGCTGTTTGTGATAGGATTTCTCGCACAGGCCGTCATGGGGATTTGACAGTTGAAGAAAGACAAATTCTACTGGCAGAGTTTTCGCTGCGTCATAGCAATAGTGCGATCAGAATTGTCGAAGATCATGCAAAGCGCAAGGCATCATTATTGACCTAG
Protein sequence

>Msa1099620

MGAEKWLDIELWDSTKECFEMLKSRGYRIATTHVGIDAVSIYDMDWSCPTAIVVGNENRGISDEALAMSDLHCSIPMKGMVDSFNVSVAAGILMHHAVCDRISRTGRHGDLTVEERQILLAEFSLRHSNSAIRIVEDHAKRKASLLT*