AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1309360

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1309360 MtrunA17_Chr8g0335951 92.075 265 17 1 277 541 190 450 3.87e-175 502
Msa1309360 MtrunA17_Chr8g0335951 60.819 342 117 4 1 342 1 325 9.61e-132 391
Msa1309360 MtrunA17_Chr8g0349421 90.984 244 18 1 300 543 1 240 1.49e-159 454
Msa1309360 MtrunA17_Chr8g0349431 98.400 125 2 0 87 211 1 125 3.30e-84 257
Msa1309360 MtrunA17_Chr1g0185601 34.186 430 273 6 79 503 36 460 1.21e-80 261
Msa1309360 MtrunA17_Chr8g0368151 33.493 418 274 2 80 495 24 439 1.18e-79 258
Msa1309360 MtrunA17_Chr1g0194521 34.038 473 296 7 48 519 68 525 2.46e-78 256
Msa1309360 MtrunA17_Chr8g0359961 32.243 428 287 3 81 507 35 460 1.39e-73 243
Msa1309360 MtrunA17_Chr2g0324251 32.957 443 285 6 74 508 27 465 1.86e-72 239
Msa1309360 MtrunA17_Chr2g0303221 32.877 438 288 6 79 514 31 464 6.43e-71 235
Msa1309360 MtrunA17_Chr4g0021331 32.569 436 282 7 79 508 27 456 1.94e-69 231
Msa1309360 MtrunA17_Chr3g0132571 27.647 510 337 8 26 526 3 489 2.08e-68 229
Msa1309360 MtrunA17_Chr2g0282091 31.368 424 284 3 72 494 26 443 7.09e-66 222
Msa1309360 MtrunA17_Chr1g0155351 28.239 301 214 2 91 390 66 365 4.22e-41 153
Msa1309360 MtrunA17_Chr2g0289531 58.730 126 23 1 351 476 1 97 1.14e-40 142
Msa1309360 MtrunA17_Chr1g0171501 25.436 401 289 7 97 497 157 547 2.89e-29 122
Msa1309360 MtrunA17_Chr7g0256181 25.180 417 300 8 81 497 160 564 9.39e-29 120
Msa1309360 MtrunA17_Chr2g0328121 22.300 426 316 7 79 497 44 461 4.59e-28 117
Msa1309360 MtrunA17_Chr1g0168811 21.543 311 232 3 143 446 211 516 8.01e-19 90.1
Msa1309360 MtrunA17_Chr1g0168811 21.751 377 247 10 125 496 159 492 6.42e-11 65.1
Msa1309360 MtrunA17_Chr3g0143411 20.263 380 288 7 123 497 155 524 1.32e-16 82.8
Msa1309360 MtrunA17_Chr3g0099661 22.324 327 245 6 113 433 170 493 2.86e-16 82.4
Msa1309360 MtrunA17_Chr3g0099661 23.282 262 166 9 177 418 582 828 3.90e-12 69.3
Msa1309360 MtrunA17_Chr3g0099661 22.581 341 240 8 178 508 344 670 3.39e-11 66.2
Msa1309360 MtrunA17_Chr4g0059671 26.172 256 179 5 183 433 175 425 2.98e-16 82.4
Msa1309360 MtrunA17_Chr8g0359551 28.502 207 147 1 242 448 203 408 3.53e-16 82.0
Msa1309360 MtrunA17_Chr8g0359551 21.852 270 210 1 183 452 214 482 5.63e-13 71.6
Msa1309360 MtrunA17_Chr1g0200211 23.754 341 246 7 183 515 175 509 3.90e-16 82.0
Msa1309360 MtrunA17_Chr8g0377751 20.669 329 224 6 159 451 674 1001 3.76e-15 79.0
Msa1309360 MtrunA17_Chr5g0446031 19.943 351 274 4 103 451 406 751 5.66e-15 78.2
Msa1309360 MtrunA17_Chr5g0446031 22.984 248 181 3 199 446 579 816 3.17e-13 72.8
Msa1309360 MtrunA17_Chr5g0446031 22.340 376 269 11 183 549 205 566 4.97e-12 68.9
Msa1309360 MtrunA17_Chr7g0220501 22.319 345 241 7 152 493 367 687 8.51e-15 77.8
Msa1309360 MtrunA17_Chr7g0220501 25.896 251 146 5 217 430 320 567 1.22e-11 67.8
Msa1309360 MtrunA17_Chr2g0290441 20.217 277 217 3 180 453 218 493 3.09e-14 75.9
Msa1309360 MtrunA17_Chr2g0290441 22.222 279 215 2 174 451 106 383 4.17e-11 65.9
Msa1309360 MtrunA17_Chr4g0033981 22.727 264 200 2 183 446 324 583 4.98e-14 75.1
Msa1309360 MtrunA17_Chr3g0126831 22.420 281 210 3 238 518 287 559 5.81e-14 75.1
Msa1309360 MtrunA17_Chr7g0241721 20.885 407 313 5 128 531 3 403 5.93e-14 74.7
Msa1309360 MtrunA17_Chr2g0297991 19.467 375 292 7 67 433 117 489 8.74e-14 74.3
Msa1309360 MtrunA17_Chr2g0297991 22.823 333 243 8 180 507 377 700 1.33e-11 67.4
Msa1309360 MtrunA17_Chr2g0297991 22.667 300 214 6 158 452 537 823 2.61e-11 66.6
Msa1309360 MtrunA17_Chr4g0037841 21.033 271 206 4 183 448 237 504 2.93e-13 72.4
Msa1309360 MtrunA17_Chr4g0060341 20.489 327 247 6 183 503 237 556 4.50e-13 72.0
Msa1309360 MtrunA17_Chr3g0136851 23.843 281 203 6 152 426 274 549 6.64e-13 72.0
Msa1309360 MtrunA17_Chr8g0368891 19.926 271 216 1 183 453 383 652 8.45e-13 71.2
Msa1309360 MtrunA17_Chr8g0392911 22.642 265 200 3 184 446 198 459 1.20e-12 70.9
Msa1309360 MtrunA17_Chr4g0054821 24.444 315 211 7 175 480 254 550 1.43e-12 70.5
Msa1309360 MtrunA17_Chr7g0214351 22.152 316 234 7 183 494 313 620 1.92e-12 70.1
Msa1309360 MtrunA17_Chr7g0256621 22.327 318 230 6 184 494 153 460 2.38e-12 69.3
Msa1309360 MtrunA17_Chr5g0396041 20.273 439 331 9 109 537 193 622 2.60e-12 70.1
Msa1309360 MtrunA17_Chr8g0353181 23.837 344 216 8 183 525 202 500 3.03e-12 69.3
Msa1309360 MtrunA17_Chr8g0382891 22.193 374 230 10 104 426 73 436 3.27e-12 68.9
Msa1309360 MtrunA17_Chr7g0220481 23.214 280 203 4 153 430 263 532 4.32e-12 68.9
Msa1309360 MtrunA17_Chr7g0215561 21.975 314 237 5 183 494 312 619 7.45e-12 68.2
Msa1309360 MtrunA17_Chr1g0162281 19.461 334 259 5 178 508 226 552 7.55e-12 68.2
Msa1309360 MtrunA17_Chr2g0331911 23.077 312 232 5 199 507 178 484 7.96e-12 68.2
Msa1309360 MtrunA17_Chr8g0387281 21.587 315 233 5 128 435 332 639 8.89e-12 67.8
Msa1309360 MtrunA17_Chr8g0387281 23.121 346 251 7 183 518 239 579 3.33e-11 66.2
Msa1309360 MtrunA17_Chr7g0246931 21.409 369 272 10 93 452 157 516 9.24e-12 67.8
Msa1309360 MtrunA17_Chr5g0394721 21.407 327 243 6 114 434 206 524 9.71e-12 67.4
Msa1309360 MtrunA17_Chr5g0394721 22.807 285 214 3 179 459 236 518 6.40e-11 65.1
Msa1309360 MtrunA17_Chr8g0369641 20.074 269 210 3 180 446 490 755 1.23e-11 67.8
Msa1309360 MtrunA17_Chr1g0210801 18.896 471 329 11 108 535 97 557 1.70e-11 67.0
Msa1309360 MtrunA17_Chr8g0391651 22.182 275 209 3 180 452 416 687 1.97e-11 67.0
Msa1309360 MtrunA17_Chr6g0478691 21.053 323 249 3 183 504 245 562 2.21e-11 66.6
Msa1309360 MtrunA17_Chr6g0458411 19.364 346 269 3 178 523 231 566 2.39e-11 66.2
Msa1309360 MtrunA17_Chr8g0365781 22.376 362 247 6 96 433 494 845 3.84e-11 66.2
Msa1309360 MtrunA17_Chr2g0332881 22.067 358 259 8 172 524 266 608 3.90e-11 65.9
Msa1309360 MtrunA17_Chr1g0170071 24.878 205 149 3 128 330 635 836 4.32e-11 65.9
Msa1309360 MtrunA17_Chr4g0038401 24.380 242 168 6 212 446 354 587 5.31e-11 65.5
Msa1309360 MtrunA17_Chr4g0060641 21.479 284 215 3 172 448 411 693 6.24e-11 65.5
Msa1309360 MtrunA17_Chr2g0285331 20.579 311 242 2 198 507 284 590 7.13e-11 65.1
Msa1309360 MtrunA17_Chr7g0241871 21.270 315 239 4 186 498 99 406 7.14e-11 65.1
Msa1309360 MtrunA17_Chr2g0296401 24.901 253 176 6 181 422 239 488 8.31e-11 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1309360 AT1G02150.1 58.527 516 203 3 10 523 3 509 0.0 633
Msa1309360 AT1G60770.1 32.289 415 277 2 81 493 25 437 1.14e-76 251
Msa1309360 AT4G02820.1 32.029 409 273 4 99 507 81 484 2.87e-74 246
Msa1309360 AT4G01990.1 33.796 432 282 3 77 507 37 465 1.48e-73 243
Msa1309360 AT2G20710.1 33.254 421 277 4 78 497 37 454 2.18e-72 239
Msa1309360 AT1G02370.1 34.524 420 260 6 81 492 73 485 2.57e-72 241
Msa1309360 AT5G27460.1 31.121 437 296 2 83 515 42 477 1.30e-68 229
Msa1309360 AT4G21705.1 30.046 436 303 2 76 510 22 456 8.59e-67 224
Msa1309360 AT2G20710.2 34.561 353 228 3 146 497 9 359 4.83e-62 209
Msa1309360 AT5G09450.1 31.746 315 207 4 71 383 52 360 7.70e-50 177
Msa1309360 AT1G28020.1 31.233 365 235 5 49 411 19 369 3.86e-45 166
Msa1309360 AT1G80270.1 27.273 385 270 6 77 460 128 503 1.69e-36 144
Msa1309360 AT1G80270.3 27.273 385 270 6 77 460 128 503 1.69e-36 144
Msa1309360 AT1G80270.2 27.273 385 270 6 77 460 128 503 1.69e-36 144
Msa1309360 AT1G80270.5 27.273 385 270 6 77 460 128 503 1.69e-36 144
Msa1309360 AT1G80270.4 27.273 385 270 6 77 460 128 503 1.69e-36 144
Msa1309360 AT1G15480.1 25.806 434 298 10 81 507 130 546 5.06e-33 133
Msa1309360 AT1G07590.1 23.587 407 303 5 96 498 82 484 2.73e-27 116
Msa1309360 AT3G11380.1 30.945 307 161 10 117 411 81 348 1.62e-25 110
Msa1309360 AT3G11380.1 30.899 178 120 3 98 273 365 541 1.22e-14 77.0
Msa1309360 AT3G15590.1 26.420 405 279 9 98 497 160 550 4.13e-25 110
Msa1309360 AT3G15590.2 26.420 405 279 9 98 497 160 550 4.13e-25 110
Msa1309360 AT5G02860.1 24.848 330 239 6 110 433 134 460 8.06e-19 90.9
Msa1309360 AT5G02860.1 23.364 321 241 2 180 500 313 628 2.06e-16 83.2
Msa1309360 AT5G02860.4 24.848 330 239 6 110 433 134 460 8.06e-19 90.9
Msa1309360 AT5G02860.4 23.364 321 241 2 180 500 313 628 2.06e-16 83.2
Msa1309360 AT5G02860.2 24.848 330 239 6 110 433 134 460 8.06e-19 90.9
Msa1309360 AT5G02860.2 23.364 321 241 2 180 500 313 628 2.06e-16 83.2
Msa1309360 AT5G02860.5 24.848 330 239 6 110 433 134 460 8.06e-19 90.9
Msa1309360 AT5G02860.5 23.364 321 241 2 180 500 313 628 2.06e-16 83.2
Msa1309360 AT5G02860.3 24.848 330 239 6 110 433 134 460 8.06e-19 90.9
Msa1309360 AT5G02860.3 23.364 321 241 2 180 500 313 628 2.06e-16 83.2
Msa1309360 AT1G28000.1 26.800 250 178 5 99 344 53 301 9.27e-19 89.0
Msa1309360 AT1G03560.1 23.792 269 203 2 180 447 256 523 1.64e-15 80.1
Msa1309360 AT1G74580.2 23.353 334 231 8 174 495 413 733 2.32e-15 79.7
Msa1309360 AT1G74580.1 23.353 334 231 8 174 495 413 733 2.32e-15 79.7
Msa1309360 AT1G74580.3 23.353 334 231 8 174 495 413 733 2.32e-15 79.7
Msa1309360 AT4G30825.1 23.077 338 229 10 130 452 542 863 3.33e-15 79.3
Msa1309360 AT3G18110.1 22.802 364 267 8 108 462 153 511 4.10e-15 79.3
Msa1309360 AT5G01110.1 21.034 290 212 6 222 508 425 700 1.08e-14 77.8
Msa1309360 AT5G01110.1 21.289 357 266 6 102 452 158 505 1.29e-11 67.8
Msa1309360 AT5G01110.2 21.034 290 212 6 222 508 453 728 1.44e-14 77.4
Msa1309360 AT5G01110.2 21.289 357 266 6 102 452 186 533 1.51e-11 67.8
Msa1309360 AT3G06920.2 19.316 497 321 9 97 522 203 690 2.15e-14 77.0
Msa1309360 AT3G06920.3 19.316 497 321 9 97 522 203 690 2.15e-14 77.0
Msa1309360 AT1G30290.2 21.408 341 258 4 188 522 284 620 2.69e-14 76.6
Msa1309360 AT1G30290.1 21.408 341 258 4 188 522 284 620 2.77e-14 76.6
Msa1309360 AT3G06920.1 19.316 497 321 9 97 522 189 676 2.92e-14 76.6
Msa1309360 AT2G18940.1 22.910 323 240 5 180 500 350 665 1.10e-13 74.7
Msa1309360 AT2G31400.2 22.050 322 210 9 183 493 411 702 2.13e-13 73.6
Msa1309360 AT2G31400.2 20.293 409 317 5 117 521 202 605 2.25e-12 70.5
Msa1309360 AT1G63150.2 24.354 271 198 5 180 447 152 418 2.23e-13 73.6
Msa1309360 AT1G63150.1 24.354 271 198 5 180 447 152 418 2.23e-13 73.6
Msa1309360 AT2G31400.1 22.050 322 210 9 183 493 411 702 2.42e-13 73.6
Msa1309360 AT2G31400.1 20.293 409 317 5 117 521 202 605 2.53e-12 70.5
Msa1309360 AT5G65560.1 23.396 265 199 4 183 446 395 656 5.55e-13 72.4
Msa1309360 AT5G65560.2 23.396 265 199 4 183 446 395 656 5.55e-13 72.4
Msa1309360 AT5G65560.3 23.396 265 199 4 183 446 395 656 5.55e-13 72.4
Msa1309360 AT2G32630.1 19.294 425 298 7 122 508 200 617 5.99e-13 72.0
Msa1309360 AT5G46580.1 22.615 283 214 4 175 453 250 531 6.63e-13 72.0
Msa1309360 AT1G43010.1 27.706 231 146 6 84 307 41 257 9.15e-13 68.9
Msa1309360 AT1G63230.1 21.118 322 240 6 180 498 9 319 1.22e-12 69.7
Msa1309360 AT3G22670.1 20.087 229 176 4 105 330 197 421 2.33e-12 70.1
Msa1309360 AT1G62590.2 22.871 411 294 8 117 518 231 627 2.47e-12 70.1
Msa1309360 AT1G62590.1 22.871 411 294 8 117 518 231 627 2.47e-12 70.1
Msa1309360 AT1G51965.1 21.322 469 295 15 109 517 113 567 3.16e-12 69.7
Msa1309360 AT5G25630.3 27.072 181 125 4 180 358 208 383 3.19e-12 69.3
Msa1309360 AT5G25630.1 27.072 181 125 4 180 358 259 434 3.20e-12 69.7
Msa1309360 AT1G20300.1 19.938 321 235 5 123 433 163 471 3.94e-12 69.3
Msa1309360 AT1G74900.1 22.096 353 256 9 110 452 52 395 4.11e-12 68.9
Msa1309360 AT2G35030.1 19.849 398 272 12 116 501 4 366 5.99e-12 68.9
Msa1309360 AT2G15630.1 20.728 357 247 5 183 507 262 614 5.99e-12 68.9
Msa1309360 AT1G63330.3 23.618 398 267 12 139 518 174 552 6.97e-12 68.6
Msa1309360 AT1G63330.1 23.618 398 267 12 139 518 174 552 6.97e-12 68.6
Msa1309360 AT1G63330.2 23.618 398 267 12 139 518 174 552 6.97e-12 68.6
Msa1309360 AT1G10910.2 19.597 347 238 5 131 440 118 460 1.11e-11 67.8
Msa1309360 AT1G10910.1 19.597 347 238 5 131 440 118 460 1.13e-11 68.2
Msa1309360 AT5G42310.1 22.699 326 237 7 180 500 373 688 1.15e-11 68.2
Msa1309360 AT5G04810.1 26.344 186 134 2 159 342 670 854 1.62e-11 67.8
Msa1309360 AT1G62910.1 21.324 408 304 7 117 518 229 625 2.17e-11 67.0
Msa1309360 AT1G63070.1 19.944 356 269 6 172 518 235 583 2.67e-11 66.6
Msa1309360 AT5G16640.1 22.059 340 254 6 116 450 86 419 3.05e-11 66.2
Msa1309360 AT1G62670.2 22.727 352 224 10 183 495 153 495 3.09e-11 66.6
Msa1309360 AT1G62670.1 22.727 352 224 10 183 495 153 495 3.09e-11 66.6
Msa1309360 AT4G20090.2 21.569 357 266 7 127 476 164 513 3.18e-11 66.6
Msa1309360 AT4G20090.2 22.277 202 156 1 183 384 329 529 8.27e-11 65.1
Msa1309360 AT4G20090.1 21.569 357 266 7 127 476 164 513 3.18e-11 66.6
Msa1309360 AT4G20090.1 22.277 202 156 1 183 384 329 529 8.27e-11 65.1
Msa1309360 AT4G31850.1 20.764 419 315 6 40 452 226 633 3.20e-11 67.0
Msa1309360 AT5G48730.1 22.455 334 243 7 150 474 149 475 3.35e-11 66.2
Msa1309360 AT3G09060.2 18.182 363 281 6 182 538 115 467 4.99e-11 65.9
Msa1309360 AT3G09060.1 18.182 363 281 6 182 538 115 467 4.99e-11 65.9
Msa1309360 AT4G39620.2 22.289 332 241 10 117 437 103 428 5.30e-11 65.5
Msa1309360 AT5G39980.1 18.750 368 288 5 180 546 259 616 7.98e-11 65.5
Msa1309360 AT4G39620.1 22.289 332 241 10 117 437 103 428 8.07e-11 65.1
Msa1309360 AT5G61990.1 20.385 260 201 3 178 433 554 811 8.49e-11 65.5

Find 111 sgRNAs with CRISPR-Local

Find 269 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATGACTTGTGTCTTTAAATT+TGG 0.114196 8_4:+5834188 Msa1309360:CDS
TATAGCATTCCATTTCAAAT+TGG 0.213868 8_4:-5830692 None:intergenic
AGCTCCCATGTAGTCGAATT+TGG 0.237732 8_4:-5833415 None:intergenic
ATCCTACCATTATTCCTAAT+TGG 0.237984 8_4:+5832981 Msa1309360:CDS
CGACTTTATCATAATCCTTA+AGG 0.296327 8_4:-5832826 None:intergenic
TCGAAGTAATGAGGAGTAAA+AGG 0.304308 8_4:+5832753 Msa1309360:CDS
TTCTGAGGCGCTTTCGTGCT+TGG 0.317772 8_4:+5833471 Msa1309360:CDS
GTACTAAATGTACTCCAATT+AGG 0.322076 8_4:-5832995 None:intergenic
AAAGGGAAAGAGAAACGTTT+TGG 0.332422 8_4:-5830519 None:intergenic
GAAACCCAAGCCAATAAAAT+TGG 0.333547 8_4:+5833528 Msa1309360:CDS
AAAACATGATAGAGCTCTTC+AGG 0.338362 8_4:+5830846 Msa1309360:CDS
TGTACTCCAATTAGGAATAA+TGG 0.340858 8_4:-5832987 None:intergenic
GATGATGGAGAATCCTGAAT+TGG 0.341809 8_4:+5830729 Msa1309360:CDS
CATTATCTCCTGAGTTTGTA+TGG 0.359485 8_4:+5833118 Msa1309360:CDS
CTGCTTCTGTGTTGAATCAA+TGG 0.369186 8_4:+5830755 Msa1309360:CDS
AAGGCCGAGGGTAGAATATT+AGG 0.374501 8_4:+5833076 Msa1309360:CDS
CTTCGTCTTGACAAAGCTTA+AGG 0.376933 8_4:-5833558 None:intergenic
ACTGACAGTGGAATTGGAAT+TGG 0.384505 8_4:+5830592 Msa1309360:CDS
ATAGCATTCCATTTCAAATT+GGG 0.388781 8_4:-5830691 None:intergenic
ATTGAGTTGTTGAAGAAGTC+GGG 0.392725 8_4:+5833607 Msa1309360:CDS
TATTTCTTCTCTCGTAAGAA+TGG 0.397940 8_4:+5833231 Msa1309360:CDS
GGGTCATATTGCTGAAAATA+GGG 0.397978 8_4:+5833447 Msa1309360:CDS
ATATATATACATATAATATC+TGG 0.400262 8_4:+5832891 Msa1309360:CDS
AGGGTCATATTGCTGAAAAT+AGG 0.400702 8_4:+5833446 Msa1309360:CDS
CCCTCTGATGATTCTAGAAT+AGG 0.408766 8_4:+5833304 Msa1309360:CDS
AGAGAAAACCCAATTTGAAA+TGG 0.410586 8_4:+5830683 Msa1309360:CDS
CCGCATACGCTTCGTCGTTA+TGG 0.412804 8_4:-5833633 None:intergenic
AACTCTGCAAAGTTCCCATT+TGG 0.415800 8_4:-5830800 None:intergenic
CAGAAAAGCTTTACGAGGAA+TGG 0.415843 8_4:+5833269 Msa1309360:CDS
GTTTCCAAGTATACCTAACT+TGG 0.418407 8_4:+5833195 Msa1309360:CDS
AAGGAAGGAATCTCACCAAA+TGG 0.426265 8_4:+5830785 Msa1309360:CDS
CACATTGACCCTTTGTTTGA+AGG 0.426662 8_4:-5830563 None:intergenic
ACAGCATGGTATCCCAAGTT+AGG 0.429036 8_4:-5833208 None:intergenic
AAGAAGATTGAATTTGAAGA+GGG 0.436849 8_4:-5830489 None:intergenic
TTAAAAGACAAGAGAACATA+TGG 0.437458 8_4:+5832674 Msa1309360:CDS
AATTGAGTTGTTGAAGAAGT+CGG 0.440073 8_4:+5833606 Msa1309360:CDS
ATTCTTTCAATCCTGTTTGA+CGG 0.440996 8_4:-5833676 None:intergenic
GAAGAGTATGAGAATAAGAA+AGG 0.442055 8_4:-5830537 None:intergenic
ACGAGGAATGGGTTTCTGTA+AGG 0.443573 8_4:+5833281 Msa1309360:CDS
TGCTTCTGTGTTGAATCAAT+GGG 0.447177 8_4:+5830756 Msa1309360:CDS
GCAAAGATGAACCGTCAAAC+AGG 0.449173 8_4:+5833665 Msa1309360:CDS
CGACAAGTGATTCAAAGAGT+TGG 0.450702 8_4:+5833506 Msa1309360:CDS
ATCTCAAAGATTCACAGTTA+TGG 0.451129 8_4:+5830646 Msa1309360:CDS
AGAGTCATCATTAAATTAAA+CGG 0.454778 8_4:-5832794 None:intergenic
CGTAAGAATGGACGATATTG+AGG 0.465330 8_4:+5833243 Msa1309360:CDS
ATACAAGAGGATTTCGATGA+TGG 0.469256 8_4:+5830714 Msa1309360:CDS
AGAAAAGCTTTACGAGGAAT+GGG 0.471834 8_4:+5833270 Msa1309360:CDS
TCAAGGATCAATAGAGAAGA+TGG 0.473452 8_4:+5832931 Msa1309360:CDS
ACAGAAGCAGAACCCAATTC+AGG 0.475894 8_4:-5830742 None:intergenic
TATCCCAAGTTAGGTATACT+TGG 0.477102 8_4:-5833199 None:intergenic
GGCTGCAATGTATATTAAGA+TGG 0.477129 8_4:+5833021 Msa1309360:CDS
GATCTAATCGCAAGAGTTCA+CGG 0.482500 8_4:+5832608 Msa1309360:CDS
GACTCTGTACATGAACCTTA+AGG 0.486004 8_4:+5832811 Msa1309360:CDS
ATGATGGAGAATCCTGAATT+GGG 0.490611 8_4:+5830730 Msa1309360:CDS
AATATCTGGTTGTCCTCTTG+TGG 0.497939 8_4:+5832905 Msa1309360:CDS
ATAAGCTGGTATCTTAAGAA+AGG 0.498356 8_4:+5833337 Msa1309360:CDS
TTATGATAAAGTCGATATGT+TGG 0.499930 8_4:+5832835 Msa1309360:CDS
GCAGATATTCTAAACCTCTC+TGG 0.506381 8_4:-5832563 None:intergenic
TTGTCCTCTTGTGGATCTCA+AGG 0.509360 8_4:+5832914 Msa1309360:CDS
ACATTGACCCTTTGTTTGAA+GGG 0.509737 8_4:-5830562 None:intergenic
TAGGAAACCTTCTCATAAGC+TGG 0.515507 8_4:+5833323 Msa1309360:CDS
CACGGAGTTTCTAGTGCAGA+AGG 0.519926 8_4:+5832626 Msa1309360:CDS
CTTCTGATGCATGTGATATG+AGG 0.519967 8_4:+5834144 Msa1309360:CDS
AAGAGTATGAGAATAAGAAA+GGG 0.530885 8_4:-5830536 None:intergenic
GACAGTGGAATTGGAATTGG+AGG 0.533917 8_4:+5830595 Msa1309360:CDS
TACATGGGAGCTTCTTTCTG+AGG 0.536998 8_4:+5833426 Msa1309360:CDS
GATGTCCAAATTCGACTACA+TGG 0.539568 8_4:+5833410 Msa1309360:CDS
CAACACTTCCATACAAACTC+AGG 0.539796 8_4:-5833126 None:intergenic
CTGAGTTTGTATGGAAGTGT+TGG 0.541520 8_4:+5833127 Msa1309360:CDS
TTATCTTCAACAAACAACAT+TGG 0.542696 8_4:+5830425 None:intergenic
AGGAAGGAATCTCACCAAAT+GGG 0.547368 8_4:+5830786 Msa1309360:CDS
TTCTTTAAGCATATGAGTGA+AGG 0.550405 8_4:+5833382 Msa1309360:CDS
TGCTGAAAATAGGGTTTCTG+AGG 0.551041 8_4:+5833456 Msa1309360:CDS
CATTGACCCTTTGTTTGAAG+GGG 0.551949 8_4:-5830561 None:intergenic
AGGACGAAGTTTACCGCGTG+TGG 0.552160 8_4:+5833155 Msa1309360:CDS
GTAAGAATGGACGATATTGA+GGG 0.554710 8_4:+5833244 Msa1309360:CDS
GTATGGAAGTGTTGGAAACA+AGG 0.554809 8_4:+5833135 Msa1309360:CDS
ATCAATGGGAGAATGAAGGA+AGG 0.564566 8_4:+5830770 Msa1309360:CDS
AAACTCAGGAGATAATGAAA+AGG 0.565979 8_4:-5833112 None:intergenic
TCGACCTAATATTCTACCCT+CGG 0.566861 8_4:-5833080 None:intergenic
AAAGAAGATTGAATTTGAAG+AGG 0.567795 8_4:-5830490 None:intergenic
AATCTTTACTCGAAGTAATG+AGG 0.567840 8_4:+5832744 Msa1309360:CDS
TCATTCTAGATCAAGAGAAA+AGG 0.571003 8_4:+5832718 Msa1309360:CDS
CAACAACTCAATTAAAACCT+CGG 0.574815 8_4:-5833596 None:intergenic
TAAGATACCAGCTTATGAGA+AGG 0.579937 8_4:-5833330 None:intergenic
TTGGAGTACATTTAGTACAA+TGG 0.583230 8_4:+5833000 Msa1309360:CDS
TTTCCAAGTATACCTAACTT+GGG 0.589423 8_4:+5833196 Msa1309360:CDS
TTTAAGCATATGAGTGAAGG+TGG 0.593303 8_4:+5833385 Msa1309360:CDS
TTGTCAAGACGAAGACGACA+TGG 0.595912 8_4:+5833567 Msa1309360:CDS
ACATGGGAGCTTCTTTCTGA+GGG 0.601564 8_4:+5833427 Msa1309360:CDS
AATGAACATATGCATTCAAG+AGG 0.604245 8_4:-5832700 None:intergenic
TCCTATTCTAGAATCATCAG+AGG 0.618071 8_4:-5833305 None:intergenic
ATAGAAAACATACACAAGGA+TGG 0.618846 8_4:-5834167 None:intergenic
ATTGACCCTTTGTTTGAAGG+GGG 0.620972 8_4:-5830560 None:intergenic
TTGAATCAATGGGAGAATGA+AGG 0.623335 8_4:+5830766 Msa1309360:CDS
AATGGAATGCTATATACAAG+AGG 0.631419 8_4:+5830701 Msa1309360:CDS
AGAAGATTGAATTTGAAGAG+GGG 0.639018 8_4:-5830488 None:intergenic
TTGACCCTTTGTTTGAAGGG+GGG 0.640200 8_4:-5830559 None:intergenic
CCTATTCTAGAATCATCAGA+GGG 0.647606 8_4:-5833304 None:intergenic
ATGAACATATGCATTCAAGA+GGG 0.654487 8_4:-5832699 None:intergenic
AAGCATATGAGTGAAGGTGG+TGG 0.657423 8_4:+5833388 Msa1309360:CDS
ATGTCCAAATTCGACTACAT+GGG 0.669288 8_4:+5833411 Msa1309360:CDS
TGATCCTTGAGATCCACAAG+AGG 0.674298 8_4:-5832918 None:intergenic
CGAGAGAAGAAATAACAGCA+TGG 0.674858 8_4:-5833222 None:intergenic
CTCCAATTAGGAATAATGGT+AGG 0.679561 8_4:-5832983 None:intergenic
TAAGAATGGACGATATTGAG+GGG 0.688947 8_4:+5833245 Msa1309360:CDS
AAGAATGGACGATATTGAGG+GGG 0.691386 8_4:+5833246 Msa1309360:CDS
ATTTATAGTTATTCCACACG+CGG 0.697764 8_4:-5833168 None:intergenic
AGACGACATGGAGAGCGCCG+AGG 0.711199 8_4:+5833579 Msa1309360:CDS
AGTCATAGAAAACATACACA+AGG 0.711577 8_4:-5834171 None:intergenic
GGATGAACAATAGACCAGAG+AGG 0.722678 8_4:+5832549 Msa1309360:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATATATACATATAATATC+TGG + chr8_4:5832891-5832910 Msa1309360:CDS 10.0%
!! TCATTATATGTTATTCTATA+TGG + chr8_4:5830885-5830904 Msa1309360:intron 15.0%
!! AAACAAAAAATATGATTGAA+AGG - chr8_4:5831848-5831867 None:intergenic 15.0%
!! AAAAAATTTATATCAGTTTG+AGG - chr8_4:5832102-5832121 None:intergenic 15.0%
!!! AAGAATTGTGTTTTTTAATC+AGG - chr8_4:5831448-5831467 None:intergenic 20.0%
!!! TAGAATTCTTGTTTATTTTC+CGG - chr8_4:5831503-5831522 None:intergenic 20.0%
!! CAACACACAAAAAAAAAAAA+AGG + chr8_4:5831664-5831683 Msa1309360:intron 20.0%
!! TTTGGAGATAGAAATTATTA+AGG - chr8_4:5831820-5831839 None:intergenic 20.0%
!!! GTTAAAGGTTTTTGATTTTT+TGG - chr8_4:5831903-5831922 None:intergenic 20.0%
!! GTAGTAAAAGAAATACATAA+CGG + chr8_4:5832069-5832088 Msa1309360:intron 20.0%
!!! GGAAATTTTCAATATATTCA+GGG - chr8_4:5832241-5832260 None:intergenic 20.0%
!!! AGGAAATTTTCAATATATTC+AGG - chr8_4:5832242-5832261 None:intergenic 20.0%
!! TGATATTAAGACATTATTTG+AGG - chr8_4:5832471-5832490 None:intergenic 20.0%
!! AGAGTCATCATTAAATTAAA+CGG - chr8_4:5832797-5832816 None:intergenic 20.0%
! AAGAAGATTGAATTTGAAGA+GGG - chr8_4:5830492-5830511 None:intergenic 25.0%
! AAAGAAGATTGAATTTGAAG+AGG - chr8_4:5830493-5830512 None:intergenic 25.0%
!! AAGAGTATGAGAATAAGAAA+GGG - chr8_4:5830539-5830558 None:intergenic 25.0%
! ATAGCATTCCATTTCAAATT+GGG - chr8_4:5830694-5830713 None:intergenic 25.0%
! TATAGCATTCCATTTCAAAT+TGG - chr8_4:5830695-5830714 None:intergenic 25.0%
!!! TGTGTTTTTTAATCAGGAAA+TGG - chr8_4:5831442-5831461 None:intergenic 25.0%
!!! AGAATTCTTGTTTATTTTCC+GGG - chr8_4:5831502-5831521 None:intergenic 25.0%
! TTAAGTTTAAATGTCTCTGT+TGG - chr8_4:5831571-5831590 None:intergenic 25.0%
! AAATCATAATGTATCATGAG+AGG - chr8_4:5831731-5831750 None:intergenic 25.0%
!! TATGATTGAAAGGTATGATT+TGG - chr8_4:5831838-5831857 None:intergenic 25.0%
! TTAAAACTTGTAGTGGTTAA+AGG - chr8_4:5831918-5831937 None:intergenic 25.0%
!! ACACAATTTAAAACTTGTAG+TGG - chr8_4:5831925-5831944 None:intergenic 25.0%
!! ACTGACCAATACAATTTTTA+GGG - chr8_4:5831975-5831994 None:intergenic 25.0%
!! ATTTCTTTTACTACGTTTCT+TGG - chr8_4:5832063-5832082 None:intergenic 25.0%
!! TAAAAGAAATACATAACGGA+CGG + chr8_4:5832073-5832092 Msa1309360:intron 25.0%
!! ACACCAACGTTAAAATTTTA+TGG + chr8_4:5832151-5832170 Msa1309360:intron 25.0%
!! CACCAACGTTAAAATTTTAT+GGG + chr8_4:5832152-5832171 Msa1309360:intron 25.0%
!! ACCAACGTTAAAATTTTATG+GGG + chr8_4:5832153-5832172 Msa1309360:intron 25.0%
!!! TTTTTTTTTACCAACAGTCT+AGG - chr8_4:5832262-5832281 None:intergenic 25.0%
! TACTTTCAAAAAATCATTCG+TGG - chr8_4:5832495-5832514 None:intergenic 25.0%
!!! TGTCTTTTAAATCATTGGTT+AGG - chr8_4:5832664-5832683 None:intergenic 25.0%
!! TCTCTTGTCTTTTAAATCAT+TGG - chr8_4:5832669-5832688 None:intergenic 25.0%
! TTAAAAGACAAGAGAACATA+TGG + chr8_4:5832674-5832693 Msa1309360:CDS 25.0%
! TTATGATAAAGTCGATATGT+TGG + chr8_4:5832835-5832854 Msa1309360:CDS 25.0%
! TTGAATGTAATAACATGTGT+TGG - chr8_4:5833855-5833874 None:intergenic 25.0%
!!! TCAAATTTTGTTTTGACTTG+CGG + chr8_4:5833977-5833996 Msa1309360:intron 25.0%
!!! AAAATGAGTTGATAGAACAT+AGG + chr8_4:5834000-5834019 Msa1309360:intron 25.0%
!!! AAATGAGTTGATAGAACATA+GGG + chr8_4:5834001-5834020 Msa1309360:intron 25.0%
!! TTCTTTTTCTCAAGCTTTTA+TGG + chr8_4:5834039-5834058 Msa1309360:intron 25.0%
!! ATGACTTGTGTCTTTAAATT+TGG + chr8_4:5834188-5834207 Msa1309360:CDS 25.0%
AGAAGATTGAATTTGAAGAG+GGG - chr8_4:5830491-5830510 None:intergenic 30.0%
! GAAGAGTATGAGAATAAGAA+AGG - chr8_4:5830540-5830559 None:intergenic 30.0%
ATCTCAAAGATTCACAGTTA+TGG + chr8_4:5830646-5830665 Msa1309360:CDS 30.0%
AGAGAAAACCCAATTTGAAA+TGG + chr8_4:5830683-5830702 Msa1309360:CDS 30.0%
AATGGAATGCTATATACAAG+AGG + chr8_4:5830701-5830720 Msa1309360:CDS 30.0%
TTGCTTCATAATGTTAAGGA+GGG - chr8_4:5831035-5831054 None:intergenic 30.0%
GTTCAATTCAATCTGTTGTT+CGG - chr8_4:5831072-5831091 None:intergenic 30.0%
TCCAAAAGAGTAAGAATGAA+CGG + chr8_4:5831092-5831111 Msa1309360:intron 30.0%
!! GAATTCTTGTTTATTTTCCG+GGG - chr8_4:5831501-5831520 None:intergenic 30.0%
CTAGAAGACATTAATGCAAT+AGG + chr8_4:5831520-5831539 Msa1309360:intron 30.0%
AATATATTCAGCTGCACAAT+TGG - chr8_4:5831618-5831637 None:intergenic 30.0%
!!! TTTTTTTGTGTGTTGCTGAT+TGG - chr8_4:5831659-5831678 None:intergenic 30.0%
AATTGTGATAGCTAAAGTGT+CGG - chr8_4:5831790-5831809 None:intergenic 30.0%
ATCCACCCTAAAAATTGTAT+TGG + chr8_4:5831967-5831986 Msa1309360:intron 30.0%
! CACTGACCAATACAATTTTT+AGG - chr8_4:5831976-5831995 None:intergenic 30.0%
! CTAAAAATTGTATTGGTCAG+TGG + chr8_4:5831974-5831993 Msa1309360:intron 30.0%
TTGGATTTCATAGATGACTA+AGG - chr8_4:5832044-5832063 None:intergenic 30.0%
TTGAAAATTTCCTAGACTGT+TGG + chr8_4:5832249-5832268 Msa1309360:intron 30.0%
ACATGTGGATAGTATAAGAA+TGG - chr8_4:5832306-5832325 None:intergenic 30.0%
TTCCGTTTAGTAGAATCATA+GGG - chr8_4:5832383-5832402 None:intergenic 30.0%
ATTCCGTTTAGTAGAATCAT+AGG - chr8_4:5832384-5832403 None:intergenic 30.0%
! TTAAGACATTATTTGAGGCA+AGG - chr8_4:5832466-5832485 None:intergenic 30.0%
! ATGTTTTGCAGGTATATGAT+TGG + chr8_4:5832528-5832547 Msa1309360:intron 30.0%
ATGAACATATGCATTCAAGA+GGG - chr8_4:5832702-5832721 None:intergenic 30.0%
AATGAACATATGCATTCAAG+AGG - chr8_4:5832703-5832722 None:intergenic 30.0%
TCATTCTAGATCAAGAGAAA+AGG + chr8_4:5832718-5832737 Msa1309360:CDS 30.0%
AATCTTTACTCGAAGTAATG+AGG + chr8_4:5832744-5832763 Msa1309360:CDS 30.0%
CGACTTTATCATAATCCTTA+AGG - chr8_4:5832829-5832848 None:intergenic 30.0%
! AGTTTTTGAACAGATGTCAA+AGG + chr8_4:5832958-5832977 Msa1309360:CDS 30.0%
ATCCTACCATTATTCCTAAT+TGG + chr8_4:5832981-5833000 Msa1309360:CDS 30.0%
TGTACTCCAATTAGGAATAA+TGG - chr8_4:5832990-5833009 None:intergenic 30.0%
GTACTAAATGTACTCCAATT+AGG - chr8_4:5832998-5833017 None:intergenic 30.0%
TTGGAGTACATTTAGTACAA+TGG + chr8_4:5833000-5833019 Msa1309360:CDS 30.0%
AAACTCAGGAGATAATGAAA+AGG - chr8_4:5833115-5833134 None:intergenic 30.0%
ATTTATAGTTATTCCACACG+CGG - chr8_4:5833171-5833190 None:intergenic 30.0%
TTTCCAAGTATACCTAACTT+GGG + chr8_4:5833196-5833215 Msa1309360:CDS 30.0%
TATTTCTTCTCTCGTAAGAA+TGG + chr8_4:5833231-5833250 Msa1309360:CDS 30.0%
ATAAGCTGGTATCTTAAGAA+AGG + chr8_4:5833337-5833356 Msa1309360:CDS 30.0%
TTCTTTAAGCATATGAGTGA+AGG + chr8_4:5833382-5833401 Msa1309360:CDS 30.0%
CAACAACTCAATTAAAACCT+CGG - chr8_4:5833599-5833618 None:intergenic 30.0%
! AATTGAGTTGTTGAAGAAGT+CGG + chr8_4:5833606-5833625 Msa1309360:CDS 30.0%
ATTCTTTCAATCCTGTTTGA+CGG - chr8_4:5833679-5833698 None:intergenic 30.0%
ATCAAAAGCTACTATCTACT+TGG - chr8_4:5833756-5833775 None:intergenic 30.0%
! ACACCTTCAATTTGAAATGT+AGG - chr8_4:5833791-5833810 None:intergenic 30.0%
!! AATGAGTTGATAGAACATAG+GGG + chr8_4:5834002-5834021 Msa1309360:intron 30.0%
CTTATCACAACAGTTATGAA+GGG + chr8_4:5834062-5834081 Msa1309360:intron 30.0%
ATAGAAAACATACACAAGGA+TGG - chr8_4:5834170-5834189 None:intergenic 30.0%
AGTCATAGAAAACATACACA+AGG - chr8_4:5834174-5834193 None:intergenic 30.0%
! AAAGGGAAAGAGAAACGTTT+TGG - chr8_4:5830522-5830541 None:intergenic 35.0%
! ACATTGACCCTTTGTTTGAA+GGG - chr8_4:5830565-5830584 None:intergenic 35.0%
! AATGTGTTTTACACTGACAG+TGG + chr8_4:5830580-5830599 Msa1309360:CDS 35.0%
! TTTTACACTGACAGTGGAAT+TGG + chr8_4:5830586-5830605 Msa1309360:CDS 35.0%
ATACAAGAGGATTTCGATGA+TGG + chr8_4:5830714-5830733 Msa1309360:CDS 35.0%
! ATGATGGAGAATCCTGAATT+GGG + chr8_4:5830730-5830749 Msa1309360:CDS 35.0%
TGCTTCTGTGTTGAATCAAT+GGG + chr8_4:5830756-5830775 Msa1309360:CDS 35.0%
TTGAATCAATGGGAGAATGA+AGG + chr8_4:5830766-5830785 Msa1309360:CDS 35.0%
AAAACATGATAGAGCTCTTC+AGG + chr8_4:5830846-5830865 Msa1309360:CDS 35.0%
ATGGTTAAAACAACTGCAGT+AGG + chr8_4:5830904-5830923 Msa1309360:intron 35.0%
!!! CCTAGCTTTTTTGGTTTTTG+CGG + chr8_4:5830927-5830946 Msa1309360:intron 35.0%
!!! TGCTAGTTTTCGCTGTTTTA+GGG + chr8_4:5830987-5831006 Msa1309360:intron 35.0%
TGCTTCATAATGTTAAGGAG+GGG - chr8_4:5831034-5831053 None:intergenic 35.0%
GTTGCTTCATAATGTTAAGG+AGG - chr8_4:5831036-5831055 None:intergenic 35.0%
! GCTGTTGCTTCATAATGTTA+AGG - chr8_4:5831039-5831058 None:intergenic 35.0%
! ACCGTTCATTCTTACTCTTT+TGG - chr8_4:5831096-5831115 None:intergenic 35.0%
! TAACATGGTAGTGTATTGGA+TGG - chr8_4:5831124-5831143 None:intergenic 35.0%
TCTTTGGTACGATAGAGATT+GGG - chr8_4:5831178-5831197 None:intergenic 35.0%
TGTTGGACCATTCCATAATA+CGG + chr8_4:5831207-5831226 Msa1309360:intron 35.0%
TTGGTCATGATATAGTTCTC+AGG - chr8_4:5831474-5831493 None:intergenic 35.0%
TTGCCAAATAGATGACACTT+TGG + chr8_4:5831749-5831768 Msa1309360:intron 35.0%
! TAGCCAAAGTGTCATCTATT+TGG - chr8_4:5831755-5831774 None:intergenic 35.0%
TTTGGACACAGATTGAACAA+TGG - chr8_4:5831885-5831904 None:intergenic 35.0%
!! GACCAATACAATTTTTAGGG+TGG - chr8_4:5831972-5831991 None:intergenic 35.0%
!! GCCCCATAAAATTTTAACGT+TGG - chr8_4:5832157-5832176 None:intergenic 35.0%
! ACGTTAAAATTTTATGGGGC+CGG + chr8_4:5832157-5832176 Msa1309360:intron 35.0%
TTACTGACAGTTTGCAAATC+TGG - chr8_4:5832334-5832353 None:intergenic 35.0%
TAGTAGAATCATAGGGGAAA+TGG - chr8_4:5832376-5832395 None:intergenic 35.0%
TCCCCTATGATTCTACTAAA+CGG + chr8_4:5832378-5832397 Msa1309360:intron 35.0%
TCCGTTTAGTAGAATCATAG+GGG - chr8_4:5832382-5832401 None:intergenic 35.0%
CATACTTTCATGCATTCCAA+AGG - chr8_4:5832411-5832430 None:intergenic 35.0%
! GACTAATGAACATGTTTTGC+AGG + chr8_4:5832517-5832536 Msa1309360:intron 35.0%
TCGAAGTAATGAGGAGTAAA+AGG + chr8_4:5832753-5832772 Msa1309360:CDS 35.0%
TCAAGGATCAATAGAGAAGA+TGG + chr8_4:5832931-5832950 Msa1309360:CDS 35.0%
CTCCAATTAGGAATAATGGT+AGG - chr8_4:5832986-5833005 None:intergenic 35.0%
GGCTGCAATGTATATTAAGA+TGG + chr8_4:5833021-5833040 Msa1309360:CDS 35.0%
!! AGCACAAGAGTGCTTAAAAA+AGG + chr8_4:5833057-5833076 Msa1309360:CDS 35.0%
CATTATCTCCTGAGTTTGTA+TGG + chr8_4:5833118-5833137 Msa1309360:CDS 35.0%
GTTTCCAAGTATACCTAACT+TGG + chr8_4:5833195-5833214 Msa1309360:CDS 35.0%
TATCCCAAGTTAGGTATACT+TGG - chr8_4:5833202-5833221 None:intergenic 35.0%
GTAAGAATGGACGATATTGA+GGG + chr8_4:5833244-5833263 Msa1309360:CDS 35.0%
TAAGAATGGACGATATTGAG+GGG + chr8_4:5833245-5833264 Msa1309360:CDS 35.0%
AGAAAAGCTTTACGAGGAAT+GGG + chr8_4:5833270-5833289 Msa1309360:CDS 35.0%
!! CCTATTCTAGAATCATCAGA+GGG - chr8_4:5833307-5833326 None:intergenic 35.0%
!! TCCTATTCTAGAATCATCAG+AGG - chr8_4:5833308-5833327 None:intergenic 35.0%
TAAGATACCAGCTTATGAGA+AGG - chr8_4:5833333-5833352 None:intergenic 35.0%
TTTAAGCATATGAGTGAAGG+TGG + chr8_4:5833385-5833404 Msa1309360:CDS 35.0%
ATGTCCAAATTCGACTACAT+GGG + chr8_4:5833411-5833430 Msa1309360:CDS 35.0%
AGGGTCATATTGCTGAAAAT+AGG + chr8_4:5833446-5833465 Msa1309360:CDS 35.0%
GGGTCATATTGCTGAAAATA+GGG + chr8_4:5833447-5833466 Msa1309360:CDS 35.0%
GAAACCCAAGCCAATAAAAT+TGG + chr8_4:5833528-5833547 Msa1309360:CDS 35.0%
! AGGAATGCAGCCAATTTTAT+TGG - chr8_4:5833541-5833560 None:intergenic 35.0%
! ATTGAGTTGTTGAAGAAGTC+GGG + chr8_4:5833607-5833626 Msa1309360:CDS 35.0%
! TTGAAATGTAGGTGCTACAT+AGG - chr8_4:5833780-5833799 None:intergenic 35.0%
GCACCTACATTTCAAATTGA+AGG + chr8_4:5833785-5833804 Msa1309360:intron 35.0%
! CATTTCAAATTGAAGGTGTG+TGG + chr8_4:5833792-5833811 Msa1309360:intron 35.0%
TGTTGGTGTAATGTCAATGT+TGG - chr8_4:5833838-5833857 None:intergenic 35.0%
!! ATGAGTTGATAGAACATAGG+GGG + chr8_4:5834003-5834022 Msa1309360:intron 35.0%
GCTTATCACAACAGTTATGA+AGG + chr8_4:5834061-5834080 Msa1309360:intron 35.0%
! GACTGATGTTTTGCTCTAAT+AGG + chr8_4:5834113-5834132 Msa1309360:intron 35.0%
! ATTGACCCTTTGTTTGAAGG+GGG - chr8_4:5830563-5830582 None:intergenic 40.0%
! CATTGACCCTTTGTTTGAAG+GGG - chr8_4:5830564-5830583 None:intergenic 40.0%
! CACATTGACCCTTTGTTTGA+AGG - chr8_4:5830566-5830585 None:intergenic 40.0%
ACTGACAGTGGAATTGGAAT+TGG + chr8_4:5830592-5830611 Msa1309360:CDS 40.0%
!! GATGATGGAGAATCCTGAAT+TGG + chr8_4:5830729-5830748 Msa1309360:CDS 40.0%
CTGCTTCTGTGTTGAATCAA+TGG + chr8_4:5830755-5830774 Msa1309360:CDS 40.0%
ATCAATGGGAGAATGAAGGA+AGG + chr8_4:5830770-5830789 Msa1309360:CDS 40.0%
AAGGAAGGAATCTCACCAAA+TGG + chr8_4:5830785-5830804 Msa1309360:CDS 40.0%
AGGAAGGAATCTCACCAAAT+GGG + chr8_4:5830786-5830805 Msa1309360:CDS 40.0%
AACTCTGCAAAGTTCCCATT+TGG - chr8_4:5830803-5830822 None:intergenic 40.0%
CCGCAAAAACCAAAAAAGCT+AGG - chr8_4:5830930-5830949 None:intergenic 40.0%
!!! CTGCTAGTTTTCGCTGTTTT+AGG + chr8_4:5830986-5831005 Msa1309360:intron 40.0%
!!! GCTAGTTTTCGCTGTTTTAG+GGG + chr8_4:5830988-5831007 Msa1309360:intron 40.0%
!! GAGGGGGTATGTTTTTAACA+CGG - chr8_4:5831017-5831036 None:intergenic 40.0%
GCTTCATAATGTTAAGGAGG+GGG - chr8_4:5831033-5831052 None:intergenic 40.0%
! AGGCTAACATGGTAGTGTAT+TGG - chr8_4:5831128-5831147 None:intergenic 40.0%
GTCTTTGGTACGATAGAGAT+TGG - chr8_4:5831179-5831198 None:intergenic 40.0%
TCACCATCCGTATTATGGAA+TGG - chr8_4:5831217-5831236 None:intergenic 40.0%
GATGATCACCATCCGTATTA+TGG - chr8_4:5831222-5831241 None:intergenic 40.0%
! TCTATTTTCAGCAGGACAAC+AGG - chr8_4:5831364-5831383 None:intergenic 40.0%
! GTTTTTACTTTGGCTGATGG+AGG - chr8_4:5831411-5831430 None:intergenic 40.0%
!!! CCTGTTTTTACTTTGGCTGA+TGG - chr8_4:5831414-5831433 None:intergenic 40.0%
CCATCAGCCAAAGTAAAAAC+AGG + chr8_4:5831411-5831430 Msa1309360:intron 40.0%
!!! GGTTGCTCCTGTTTTTACTT+TGG - chr8_4:5831421-5831440 None:intergenic 40.0%
ATATCATGACCAAACTACCC+CGG + chr8_4:5831481-5831500 Msa1309360:intron 40.0%
!! GTTTATTTTCCGGGGTAGTT+TGG - chr8_4:5831493-5831512 None:intergenic 40.0%
! TTAACAGCTTTTGCAGTGAC+TGG + chr8_4:5831587-5831606 Msa1309360:intron 40.0%
! TAGGGTGGATTTCATAGTCT+TGG - chr8_4:5831957-5831976 None:intergenic 40.0%
TTAACGTTGGTGTGAGAACA+TGG - chr8_4:5832144-5832163 None:intergenic 40.0%
TACCACGTCAAAAGTTTAGC+CGG - chr8_4:5832179-5832198 None:intergenic 40.0%
! GCAAATCTGGAATTGACATG+TGG - chr8_4:5832321-5832340 None:intergenic 40.0%
ACTAAACGGAATGCTTCCTT+TGG + chr8_4:5832392-5832411 Msa1309360:intron 40.0%
GCAGATATTCTAAACCTCTC+TGG - chr8_4:5832566-5832585 None:intergenic 40.0%
GATCTAATCGCAAGAGTTCA+CGG + chr8_4:5832608-5832627 Msa1309360:CDS 40.0%
GACTCTGTACATGAACCTTA+AGG + chr8_4:5832811-5832830 Msa1309360:CDS 40.0%
AATATCTGGTTGTCCTCTTG+TGG + chr8_4:5832905-5832924 Msa1309360:CDS 40.0%
TCGACCTAATATTCTACCCT+CGG - chr8_4:5833083-5833102 None:intergenic 40.0%
CAACACTTCCATACAAACTC+AGG - chr8_4:5833129-5833148 None:intergenic 40.0%
! CTGAGTTTGTATGGAAGTGT+TGG + chr8_4:5833127-5833146 Msa1309360:CDS 40.0%
!! GTATGGAAGTGTTGGAAACA+AGG + chr8_4:5833135-5833154 Msa1309360:CDS 40.0%
CGAGAGAAGAAATAACAGCA+TGG - chr8_4:5833225-5833244 None:intergenic 40.0%
CGTAAGAATGGACGATATTG+AGG + chr8_4:5833243-5833262 Msa1309360:CDS 40.0%
AAGAATGGACGATATTGAGG+GGG + chr8_4:5833246-5833265 Msa1309360:CDS 40.0%
CAGAAAAGCTTTACGAGGAA+TGG + chr8_4:5833269-5833288 Msa1309360:CDS 40.0%
!! CCCTCTGATGATTCTAGAAT+AGG + chr8_4:5833304-5833323 Msa1309360:CDS 40.0%
TAGGAAACCTTCTCATAAGC+TGG + chr8_4:5833323-5833342 Msa1309360:CDS 40.0%
GATGTCCAAATTCGACTACA+TGG + chr8_4:5833410-5833429 Msa1309360:CDS 40.0%
TGCTGAAAATAGGGTTTCTG+AGG + chr8_4:5833456-5833475 Msa1309360:CDS 40.0%
CGACAAGTGATTCAAAGAGT+TGG + chr8_4:5833506-5833525 Msa1309360:CDS 40.0%
!!! GCAGCCAATTTTATTGGCTT+GGG - chr8_4:5833535-5833554 None:intergenic 40.0%
!! TGCAGCCAATTTTATTGGCT+TGG - chr8_4:5833536-5833555 None:intergenic 40.0%
CTTCGTCTTGACAAAGCTTA+AGG - chr8_4:5833561-5833580 None:intergenic 40.0%
CTAGGTTCTTATGCTAGGTT+AGG + chr8_4:5834085-5834104 Msa1309360:intron 40.0%
TCTTATGCTAGGTTAGGACA+TGG + chr8_4:5834091-5834110 Msa1309360:intron 40.0%
CTTCTGATGCATGTGATATG+AGG + chr8_4:5834144-5834163 Msa1309360:CDS 40.0%
TCTTCCCCCCTTCAAACAAA+GGG + chr8_4:5830555-5830574 Msa1309360:CDS 45.0%
! TTGACCCTTTGTTTGAAGGG+GGG - chr8_4:5830562-5830581 None:intergenic 45.0%
GACAGTGGAATTGGAATTGG+AGG + chr8_4:5830595-5830614 Msa1309360:CDS 45.0%
ACAGAAGCAGAACCCAATTC+AGG - chr8_4:5830745-5830764 None:intergenic 45.0%
!!! TGCAGTAGGCCTAGCTTTTT+TGG + chr8_4:5830918-5830937 Msa1309360:intron 45.0%
!!! TTGGTTTTTGCGGTGCTGTT+TGG + chr8_4:5830937-5830956 Msa1309360:intron 45.0%
CATCTACAGTGAGGCTAACA+TGG - chr8_4:5831139-5831158 None:intergenic 45.0%
CGTAGTTCACATCTACAGTG+AGG - chr8_4:5831148-5831167 None:intergenic 45.0%
TTGGTACGATAGAGATTGGG+AGG - chr8_4:5831175-5831194 None:intergenic 45.0%
ATGGAATGGTCCAACAACAG+TGG - chr8_4:5831203-5831222 None:intergenic 45.0%
GGACCATTCCATAATACGGA+TGG + chr8_4:5831211-5831230 Msa1309360:intron 45.0%
! AAGCCTTATCCCACTAAGTG+GGG - chr8_4:5831259-5831278 None:intergenic 45.0%
! CAAGCCTTATCCCACTAAGT+GGG - chr8_4:5831260-5831279 None:intergenic 45.0%
! GGAGGGATTCTATTTTCAGC+AGG - chr8_4:5831372-5831391 None:intergenic 45.0%
GGATGAACAATAGACCAGAG+AGG + chr8_4:5832549-5832568 Msa1309360:CDS 45.0%
TTGTCCTCTTGTGGATCTCA+AGG + chr8_4:5832914-5832933 Msa1309360:CDS 45.0%
TGATCCTTGAGATCCACAAG+AGG - chr8_4:5832921-5832940 None:intergenic 45.0%
! AGAGTGCTTAAAAAAGGCCG+AGG + chr8_4:5833063-5833082 Msa1309360:CDS 45.0%
GAGTGCTTAAAAAAGGCCGA+GGG + chr8_4:5833064-5833083 Msa1309360:CDS 45.0%
AAGGCCGAGGGTAGAATATT+AGG + chr8_4:5833076-5833095 Msa1309360:CDS 45.0%
ACAGCATGGTATCCCAAGTT+AGG - chr8_4:5833211-5833230 None:intergenic 45.0%
! ACGAGGAATGGGTTTCTGTA+AGG + chr8_4:5833281-5833300 Msa1309360:CDS 45.0%
AAGCATATGAGTGAAGGTGG+TGG + chr8_4:5833388-5833407 Msa1309360:CDS 45.0%
AGCTCCCATGTAGTCGAATT+TGG - chr8_4:5833418-5833437 None:intergenic 45.0%
TACATGGGAGCTTCTTTCTG+AGG + chr8_4:5833426-5833445 Msa1309360:CDS 45.0%
ACATGGGAGCTTCTTTCTGA+GGG + chr8_4:5833427-5833446 Msa1309360:CDS 45.0%
TTGTCAAGACGAAGACGACA+TGG + chr8_4:5833567-5833586 Msa1309360:CDS 45.0%
GCAAAGATGAACCGTCAAAC+AGG + chr8_4:5833665-5833684 Msa1309360:CDS 45.0%
GTGTGGTGTCTAACATGTGT+CGG + chr8_4:5833809-5833828 Msa1309360:intron 45.0%
CACAACAGTTATGAAGGGCT+AGG + chr8_4:5834067-5834086 Msa1309360:intron 45.0%
AAGGGCTAGGTTCTTATGCT+AGG + chr8_4:5834080-5834099 Msa1309360:intron 45.0%
!!! TATAAATTTTTTAATTCAAA+AGG + chr8_4:5832110-5832129 Msa1309360:intron 5.0%
CTCTTCCCCCCTTCAAACAA+AGG + chr8_4:5830554-5830573 Msa1309360:CDS 50.0%
ACCAAAGACGCCACTGTTGT+TGG + chr8_4:5831190-5831209 Msa1309360:intron 50.0%
TCCAACAACAGTGGCGTCTT+TGG - chr8_4:5831194-5831213 None:intergenic 50.0%
TGTTATCCGACCCCACTTAG+TGG + chr8_4:5831246-5831265 Msa1309360:intron 50.0%
GTTATCCGACCCCACTTAGT+GGG + chr8_4:5831247-5831266 Msa1309360:intron 50.0%
CTTATCCCACTAAGTGGGGT+CGG - chr8_4:5831255-5831274 None:intergenic 50.0%
! CCAAGCCTTATCCCACTAAG+TGG - chr8_4:5831261-5831280 None:intergenic 50.0%
CCACTTAGTGGGATAAGGCT+TGG + chr8_4:5831258-5831277 Msa1309360:intron 50.0%
CTCCACCAGAACTGCTTCAT+TGG + chr8_4:5831338-5831357 Msa1309360:intron 50.0%
ACAGGCCAATGAAGCAGTTC+TGG - chr8_4:5831346-5831365 None:intergenic 50.0%
! ACTTTGGCTGATGGAGGCAT+CGG - chr8_4:5831405-5831424 None:intergenic 50.0%
GTCAGTGGTGACTCAGTTGA+TGG + chr8_4:5831989-5832008 Msa1309360:intron 50.0%
ACGTTGGTGTGAGAACATGG+AGG - chr8_4:5832141-5832160 None:intergenic 50.0%
!! CACGGAGTTTCTAGTGCAGA+AGG + chr8_4:5832626-5832645 Msa1309360:CDS 50.0%
CCATAACGACGAAGCGTATG+CGG + chr8_4:5833633-5833652 Msa1309360:CDS 50.0%
TCCTCGATGTGTGTGTGTGT+GGG + chr8_4:5833935-5833954 Msa1309360:intron 50.0%
CGACCCCACTTAGTGGGATA+AGG + chr8_4:5831253-5831272 Msa1309360:intron 55.0%
! GGCCAATGAAGCAGTTCTGG+TGG - chr8_4:5831343-5831362 None:intergenic 55.0%
!! GCATCGGTGACTGGTTTGGA+GGG - chr8_4:5831389-5831408 None:intergenic 55.0%
! GGCCGGCTAAACTTTTGACG+TGG + chr8_4:5832174-5832193 Msa1309360:intron 55.0%
AGGACGAAGTTTACCGCGTG+TGG + chr8_4:5833155-5833174 Msa1309360:CDS 55.0%
GGGGGCAGAAAAGCTTTACG+AGG + chr8_4:5833264-5833283 Msa1309360:CDS 55.0%
!! TTCTGAGGCGCTTTCGTGCT+TGG + chr8_4:5833471-5833490 Msa1309360:CDS 55.0%
CCGCATACGCTTCGTCGTTA+TGG - chr8_4:5833636-5833655 None:intergenic 55.0%
GTCCTCGATGTGTGTGTGTG+TGG + chr8_4:5833934-5833953 Msa1309360:intron 55.0%
ACCCACACACACACACATCG+AGG - chr8_4:5833939-5833958 None:intergenic 55.0%
!! GGCATCGGTGACTGGTTTGG+AGG - chr8_4:5831390-5831409 None:intergenic 60.0%
!! GGAGGCATCGGTGACTGGTT+TGG - chr8_4:5831393-5831412 None:intergenic 60.0%
!! CTGATGGAGGCATCGGTGAC+TGG - chr8_4:5831398-5831417 None:intergenic 60.0%
!! AGACGACATGGAGAGCGCCG+AGG + chr8_4:5833579-5833598 Msa1309360:CDS 65.0%
Chromosome Type Strat End Strand Name
chr8_4 gene 5830429 5834220 5830429 ID=Msa1309360;Name=Msa1309360
chr8_4 mRNA 5830429 5834220 5830429 ID=Msa1309360-mRNA-1;Parent=Msa1309360;Name=Msa1309360-mRNA-1;_AED=0.15;_eAED=0.15;_QI=43|0.5|0.33|1|1|1|3|0|551
chr8_4 exon 5830429 5830867 5830429 ID=Msa1309360-mRNA-1:exon:1922;Parent=Msa1309360-mRNA-1
chr8_4 exon 5832539 5833712 5832539 ID=Msa1309360-mRNA-1:exon:1923;Parent=Msa1309360-mRNA-1
chr8_4 exon 5834135 5834220 5834135 ID=Msa1309360-mRNA-1:exon:1924;Parent=Msa1309360-mRNA-1
chr8_4 five_prime_UTR 5830429 5830471 5830429 ID=Msa1309360-mRNA-1:five_prime_utr;Parent=Msa1309360-mRNA-1
chr8_4 CDS 5830472 5830867 5830472 ID=Msa1309360-mRNA-1:cds;Parent=Msa1309360-mRNA-1
chr8_4 CDS 5832539 5833712 5832539 ID=Msa1309360-mRNA-1:cds;Parent=Msa1309360-mRNA-1
chr8_4 CDS 5834135 5834220 5834135 ID=Msa1309360-mRNA-1:cds;Parent=Msa1309360-mRNA-1
Gene Sequence

>Msa1309360

ATGATGACTACTATTACCCCTCTTCAAATTCAATCTTCTTTACAAAACCAAAACGTTTCTCTTTCCCTTTCTTATTCTCATACTCTTCCCCCCTTCAAACAAAGGGTCAATGTGTTTTACACTGACAGTGGAATTGGAATTGGAGGAGCAAGAAGAGTTTCTGTCACTTGTTCAATCTCAAAGATTCACAGTTATGGTACACTTGATTATGAGAGAAAACCCAATTTGAAATGGAATGCTATATACAAGAGGATTTCGATGATGGAGAATCCTGAATTGGGTTCTGCTTCTGTGTTGAATCAATGGGAGAATGAAGGAAGGAATCTCACCAAATGGGAACTTTGCAGAGTTGTTAAAGAGTTGAGAAAGTACAGAAAACATGATAGAGCTCTTCAGGTATATGATTGGATGAACAATAGACCAGAGAGGTTTAGAATATCTGCAAGTGACGTTGCGATTCAGTTAGATCTAATCGCAAGAGTTCACGGAGTTTCTAGTGCAGAAGGTTTTTTTCTGAACCTAACCAATGATTTAAAAGACAAGAGAACATATGGTGCCCTCTTGAATGCATATGTTCATTCTAGATCAAGAGAAAAGGCAGAATCTTTACTCGAAGTAATGAGGAGTAAAAGGTACTTAATACATTCACTACCGTTTAATTTAATGATGACTCTGTACATGAACCTTAAGGATTATGATAAAGTCGATATGTTGGTTTCAGAAATGAAAGAAAAGAACATACAGCTAGATATATATACATATAATATCTGGTTGTCCTCTTGTGGATCTCAAGGATCAATAGAGAAGATGGAACAAGTTTTTGAACAGATGTCAAAGGATCCTACCATTATTCCTAATTGGAGTACATTTAGTACAATGGCTGCAATGTATATTAAGATGGAACTGTTTGAAAAAGCACAAGAGTGCTTAAAAAAGGCCGAGGGTAGAATATTAGGTCGAGATAAAGTACCTTTTCATTATCTCCTGAGTTTGTATGGAAGTGTTGGAAACAAGGACGAAGTTTACCGCGTGTGGAATAACTATAAATCAATGTTTCCAAGTATACCTAACTTGGGATACCATGCTGTTATTTCTTCTCTCGTAAGAATGGACGATATTGAGGGGGCAGAAAAGCTTTACGAGGAATGGGTTTCTGTAAGGCCCTCTGATGATTCTAGAATAGGAAACCTTCTCATAAGCTGGTATCTTAAGAAAGGTAAATCAGATAAAGTTTTTAGCTTCTTTAAGCATATGAGTGAAGGTGGTGGATGTCCAAATTCGACTACATGGGAGCTTCTTTCTGAGGGTCATATTGCTGAAAATAGGGTTTCTGAGGCGCTTTCGTGCTTGGAAAAAGCTTTTACGACAAGTGATTCAAAGAGTTGGAAACCCAAGCCAATAAAATTGGCTGCATTCCTTAAGCTTTGTCAAGACGAAGACGACATGGAGAGCGCCGAGGTTTTAATTGAGTTGTTGAAGAAGTCGGGATACCATAACGACGAAGCGTATGCGGCACTCATTAGCAAAGATGAACCGTCAAACAGGATTGAAAGAATTGATGATGTTGACAGAATTGACTCTTCTGATGCATGTGATATGAGGCCATCCTTGTGTATGTTTTCTATGACTTGTGTCTTTAAATTTGGAATGAAGTAG
Protein sequence

>Msa1309360

MMTTITPLQIQSSLQNQNVSLSLSYSHTLPPFKQRVNVFYTDSGIGIGGARRVSVTCSISKIHSYGTLDYERKPNLKWNAIYKRISMMENPELGSASVLNQWENEGRNLTKWELCRVVKELRKYRKHDRALQVYDWMNNRPERFRISASDVAIQLDLIARVHGVSSAEGFFLNLTNDLKDKRTYGALLNAYVHSRSREKAESLLEVMRSKRYLIHSLPFNLMMTLYMNLKDYDKVDMLVSEMKEKNIQLDIYTYNIWLSSCGSQGSIEKMEQVFEQMSKDPTIIPNWSTFSTMAAMYIKMELFEKAQECLKKAEGRILGRDKVPFHYLLSLYGSVGNKDEVYRVWNNYKSMFPSIPNLGYHAVISSLVRMDDIEGAEKLYEEWVSVRPSDDSRIGNLLISWYLKKGKSDKVFSFFKHMSEGGGCPNSTTWELLSEGHIAENRVSEALSCLEKAFTTSDSKSWKPKPIKLAAFLKLCQDEDDMESAEVLIELLKKSGYHNDEAYAALISKDEPSNRIERIDDVDRIDSSDACDMRPSLCMFSMTCVFKFGMK*