AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1229050

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1229050 MtrunA17_Chr8g0335951 91.698 265 18 1 277 541 190 450 1.76e-174 500
Msa1229050 MtrunA17_Chr8g0335951 60.819 342 117 4 1 342 1 325 1.01e-131 391
Msa1229050 MtrunA17_Chr8g0349421 90.574 244 19 1 300 543 1 240 5.45e-159 453
Msa1229050 MtrunA17_Chr8g0349431 98.400 125 2 0 87 211 1 125 3.41e-84 257
Msa1229050 MtrunA17_Chr1g0185601 34.186 430 273 6 79 503 36 460 1.21e-80 261
Msa1229050 MtrunA17_Chr8g0368151 33.493 418 274 2 80 495 24 439 9.86e-80 258
Msa1229050 MtrunA17_Chr1g0194521 34.038 473 296 7 48 519 68 525 1.73e-78 256
Msa1229050 MtrunA17_Chr8g0359961 32.243 428 287 3 81 507 35 460 9.85e-74 243
Msa1229050 MtrunA17_Chr2g0324251 32.957 443 285 6 74 508 27 465 1.75e-72 239
Msa1229050 MtrunA17_Chr2g0303221 32.877 438 288 6 79 514 31 464 4.45e-71 236
Msa1229050 MtrunA17_Chr4g0021331 32.569 436 282 7 79 508 27 456 1.23e-69 232
Msa1229050 MtrunA17_Chr3g0132571 27.647 510 337 8 26 526 3 489 1.60e-68 229
Msa1229050 MtrunA17_Chr2g0282091 31.368 424 284 3 72 494 26 443 6.66e-66 222
Msa1229050 MtrunA17_Chr1g0155351 28.239 301 214 2 91 390 66 365 3.63e-41 153
Msa1229050 MtrunA17_Chr2g0289531 58.730 126 23 1 351 476 1 97 1.14e-40 142
Msa1229050 MtrunA17_Chr1g0171501 25.432 405 292 7 93 497 153 547 2.36e-29 122
Msa1229050 MtrunA17_Chr7g0256181 25.180 417 300 8 81 497 160 564 6.88e-29 121
Msa1229050 MtrunA17_Chr2g0328121 22.300 426 316 7 79 497 44 461 4.10e-28 117
Msa1229050 MtrunA17_Chr1g0168811 21.543 311 232 3 143 446 211 516 8.98e-19 90.1
Msa1229050 MtrunA17_Chr1g0168811 21.751 377 247 10 125 496 159 492 5.12e-11 65.5
Msa1229050 MtrunA17_Chr3g0143411 20.263 380 288 7 123 497 155 524 1.12e-16 83.2
Msa1229050 MtrunA17_Chr3g0099661 22.324 327 245 6 113 433 170 493 2.81e-16 82.4
Msa1229050 MtrunA17_Chr3g0099661 23.282 262 166 9 177 418 582 828 3.93e-12 69.3
Msa1229050 MtrunA17_Chr3g0099661 23.385 325 223 9 178 491 344 653 4.13e-11 65.9
Msa1229050 MtrunA17_Chr4g0059671 26.172 256 179 5 183 433 175 425 2.83e-16 82.4
Msa1229050 MtrunA17_Chr8g0359551 28.502 207 147 1 242 448 203 408 3.50e-16 82.0
Msa1229050 MtrunA17_Chr8g0359551 21.852 270 210 1 183 452 214 482 5.21e-13 71.6
Msa1229050 MtrunA17_Chr1g0200211 23.754 341 246 7 183 515 175 509 3.63e-16 82.0
Msa1229050 MtrunA17_Chr7g0220501 22.609 345 240 7 152 493 367 687 2.56e-15 79.3
Msa1229050 MtrunA17_Chr7g0220501 25.896 251 146 5 217 430 320 567 1.26e-11 67.8
Msa1229050 MtrunA17_Chr8g0377751 20.669 329 224 6 159 451 674 1001 3.86e-15 79.0
Msa1229050 MtrunA17_Chr5g0446031 19.943 351 274 4 103 451 406 751 5.05e-15 78.6
Msa1229050 MtrunA17_Chr5g0446031 22.984 248 181 3 199 446 579 816 3.25e-13 72.8
Msa1229050 MtrunA17_Chr5g0446031 22.340 376 269 11 183 549 205 566 3.64e-12 69.3
Msa1229050 MtrunA17_Chr2g0290441 20.217 277 217 3 180 453 218 493 3.31e-14 75.5
Msa1229050 MtrunA17_Chr2g0290441 22.222 279 215 2 174 451 106 383 4.32e-11 65.9
Msa1229050 MtrunA17_Chr3g0126831 22.420 281 210 3 238 518 287 559 4.00e-14 75.5
Msa1229050 MtrunA17_Chr4g0033981 22.727 264 200 2 183 446 324 583 4.56e-14 75.1
Msa1229050 MtrunA17_Chr7g0241721 20.885 407 313 5 128 531 3 403 4.93e-14 75.1
Msa1229050 MtrunA17_Chr2g0297991 19.467 375 292 7 67 433 117 489 7.74e-14 74.7
Msa1229050 MtrunA17_Chr2g0297991 22.823 333 243 8 180 507 377 700 1.35e-11 67.4
Msa1229050 MtrunA17_Chr2g0297991 22.667 300 214 6 158 452 537 823 2.50e-11 66.6
Msa1229050 MtrunA17_Chr4g0037841 20.795 327 246 6 183 503 237 556 1.56e-13 73.2
Msa1229050 MtrunA17_Chr4g0060341 20.795 327 246 6 183 503 237 556 1.69e-13 73.2
Msa1229050 MtrunA17_Chr3g0136851 23.843 281 203 6 152 426 274 549 5.99e-13 72.0
Msa1229050 MtrunA17_Chr8g0368891 19.926 271 216 1 183 453 383 652 8.30e-13 71.2
Msa1229050 MtrunA17_Chr5g0396041 20.501 439 330 9 109 537 193 622 8.42e-13 71.6
Msa1229050 MtrunA17_Chr8g0392911 22.642 265 200 3 184 446 198 459 1.19e-12 70.9
Msa1229050 MtrunA17_Chr8g0353181 23.615 343 218 7 183 525 202 500 1.31e-12 70.5
Msa1229050 MtrunA17_Chr4g0054821 24.522 314 210 7 175 479 254 549 2.15e-12 70.1
Msa1229050 MtrunA17_Chr8g0382891 22.193 374 230 10 104 426 73 436 2.99e-12 68.9
Msa1229050 MtrunA17_Chr7g0220481 23.214 280 203 4 153 430 263 532 3.83e-12 69.3
Msa1229050 MtrunA17_Chr8g0369641 19.935 306 236 4 180 483 490 788 4.48e-12 68.9
Msa1229050 MtrunA17_Chr2g0331911 23.077 312 232 5 199 507 178 484 7.04e-12 68.2
Msa1229050 MtrunA17_Chr1g0162281 20.060 334 257 5 178 508 226 552 8.45e-12 68.2
Msa1229050 MtrunA17_Chr8g0387281 21.587 315 233 5 128 435 332 639 8.51e-12 67.8
Msa1229050 MtrunA17_Chr8g0387281 23.410 346 250 7 183 518 239 579 9.45e-12 67.8
Msa1229050 MtrunA17_Chr7g0214351 21.835 316 235 7 183 494 313 620 8.87e-12 67.8
Msa1229050 MtrunA17_Chr5g0394721 21.407 327 243 6 114 434 206 524 9.54e-12 67.8
Msa1229050 MtrunA17_Chr5g0394721 22.807 285 214 3 179 459 236 518 6.68e-11 65.1
Msa1229050 MtrunA17_Chr7g0246931 21.409 369 272 10 93 452 157 516 9.57e-12 67.4
Msa1229050 MtrunA17_Chr7g0256621 22.013 318 231 6 184 494 153 460 1.36e-11 67.0
Msa1229050 MtrunA17_Chr1g0210801 18.896 471 329 11 108 535 97 557 1.58e-11 67.0
Msa1229050 MtrunA17_Chr6g0478691 21.053 323 249 3 183 504 245 562 1.70e-11 67.0
Msa1229050 MtrunA17_Chr2g0285331 20.900 311 241 2 198 507 284 590 1.87e-11 67.0
Msa1229050 MtrunA17_Chr8g0391651 22.182 275 209 3 180 452 416 687 2.01e-11 67.0
Msa1229050 MtrunA17_Chr2g0332881 22.067 358 259 8 172 524 266 608 2.44e-11 66.6
Msa1229050 MtrunA17_Chr6g0458411 20.173 347 265 5 178 523 231 566 2.63e-11 66.2
Msa1229050 MtrunA17_Chr6g0458411 22.857 385 244 9 184 523 98 474 4.67e-11 65.5
Msa1229050 MtrunA17_Chr7g0215561 21.656 314 238 5 183 494 312 619 3.49e-11 65.9
Msa1229050 MtrunA17_Chr8g0365781 22.376 362 247 6 96 433 494 845 3.67e-11 66.2
Msa1229050 MtrunA17_Chr1g0170071 26.380 163 117 2 170 330 675 836 4.55e-11 65.9
Msa1229050 MtrunA17_Chr4g0038401 24.380 242 168 6 212 446 354 587 5.55e-11 65.5
Msa1229050 MtrunA17_Chr4g0060641 20.760 342 255 5 172 506 411 743 5.73e-11 65.5
Msa1229050 MtrunA17_Chr7g0241871 21.270 315 239 4 186 498 99 406 5.90e-11 65.5
Msa1229050 MtrunA17_Chr2g0296401 24.901 253 176 6 181 422 239 488 8.24e-11 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1229050 AT1G02150.1 58.527 516 203 3 10 523 3 509 0.0 633
Msa1229050 AT1G60770.1 32.289 415 277 2 81 493 25 437 9.75e-77 251
Msa1229050 AT4G02820.1 32.029 409 273 4 99 507 81 484 2.00e-74 246
Msa1229050 AT4G01990.1 33.796 432 282 3 77 507 37 465 1.24e-73 243
Msa1229050 AT2G20710.1 33.254 421 277 4 78 497 37 454 1.21e-72 240
Msa1229050 AT1G02370.1 34.524 420 260 6 81 492 73 485 1.78e-72 241
Msa1229050 AT5G27460.1 31.121 437 296 2 83 515 42 477 2.70e-68 229
Msa1229050 AT4G21705.1 30.046 436 303 2 76 510 22 456 7.19e-67 225
Msa1229050 AT2G20710.2 34.561 353 228 3 146 497 9 359 2.39e-62 210
Msa1229050 AT5G09450.1 31.746 315 207 4 71 383 52 360 7.10e-50 177
Msa1229050 AT1G28020.1 31.233 365 235 5 49 411 19 369 3.63e-45 166
Msa1229050 AT1G80270.1 27.273 385 270 6 77 460 128 503 2.01e-36 143
Msa1229050 AT1G80270.3 27.273 385 270 6 77 460 128 503 2.01e-36 143
Msa1229050 AT1G80270.2 27.273 385 270 6 77 460 128 503 2.01e-36 143
Msa1229050 AT1G80270.5 27.273 385 270 6 77 460 128 503 2.01e-36 143
Msa1229050 AT1G80270.4 27.273 385 270 6 77 460 128 503 2.01e-36 143
Msa1229050 AT1G15480.1 25.806 434 298 10 81 507 130 546 4.08e-33 134
Msa1229050 AT1G07590.1 23.587 407 303 5 96 498 82 484 2.11e-27 116
Msa1229050 AT3G11380.1 30.945 307 161 10 117 411 81 348 1.65e-25 110
Msa1229050 AT3G11380.1 30.899 178 120 3 98 273 365 541 1.25e-14 77.0
Msa1229050 AT3G15590.1 26.173 405 280 9 98 497 160 550 2.99e-25 110
Msa1229050 AT3G15590.2 26.173 405 280 9 98 497 160 550 2.99e-25 110
Msa1229050 AT5G02860.1 24.848 330 239 6 110 433 134 460 8.72e-19 90.9
Msa1229050 AT5G02860.1 23.053 321 242 2 180 500 313 628 8.90e-16 81.3
Msa1229050 AT5G02860.4 24.848 330 239 6 110 433 134 460 8.72e-19 90.9
Msa1229050 AT5G02860.4 23.053 321 242 2 180 500 313 628 8.90e-16 81.3
Msa1229050 AT5G02860.2 24.848 330 239 6 110 433 134 460 8.72e-19 90.9
Msa1229050 AT5G02860.2 23.053 321 242 2 180 500 313 628 8.90e-16 81.3
Msa1229050 AT5G02860.5 24.848 330 239 6 110 433 134 460 8.72e-19 90.9
Msa1229050 AT5G02860.5 23.053 321 242 2 180 500 313 628 8.90e-16 81.3
Msa1229050 AT5G02860.3 24.848 330 239 6 110 433 134 460 8.72e-19 90.9
Msa1229050 AT5G02860.3 23.053 321 242 2 180 500 313 628 8.90e-16 81.3
Msa1229050 AT1G28000.1 26.800 250 178 5 99 344 53 301 9.35e-19 89.0
Msa1229050 AT1G03560.1 23.792 269 203 2 180 447 256 523 1.64e-15 80.1
Msa1229050 AT4G30825.1 23.077 338 229 10 130 452 542 863 2.85e-15 79.7
Msa1229050 AT1G74580.2 22.222 333 236 8 174 495 413 733 3.01e-15 79.3
Msa1229050 AT1G74580.1 22.222 333 236 8 174 495 413 733 3.01e-15 79.3
Msa1229050 AT1G74580.3 22.222 333 236 8 174 495 413 733 3.01e-15 79.3
Msa1229050 AT5G01110.1 21.379 290 211 6 222 508 425 700 3.21e-15 79.3
Msa1229050 AT5G01110.1 21.289 357 266 6 102 452 158 505 1.37e-11 67.8
Msa1229050 AT3G18110.1 22.529 435 320 10 108 535 153 577 4.07e-15 79.3
Msa1229050 AT5G01110.2 21.379 290 211 6 222 508 453 728 4.32e-15 79.0
Msa1229050 AT5G01110.2 21.289 357 266 6 102 452 186 533 1.63e-11 67.8
Msa1229050 AT1G30290.2 21.408 341 258 4 188 522 284 620 1.71e-14 77.0
Msa1229050 AT1G30290.1 21.408 341 258 4 188 522 284 620 2.06e-14 77.0
Msa1229050 AT2G18940.1 23.220 323 239 5 180 500 350 665 3.09e-14 76.3
Msa1229050 AT3G06920.2 19.828 464 321 10 97 521 203 654 3.75e-14 76.3
Msa1229050 AT3G06920.3 19.828 464 321 10 97 521 203 654 3.75e-14 76.3
Msa1229050 AT3G06920.1 19.785 465 322 10 97 522 189 641 4.66e-14 75.9
Msa1229050 AT2G31400.2 22.360 322 209 9 183 493 411 702 5.63e-14 75.5
Msa1229050 AT2G31400.2 20.782 409 315 5 117 521 202 605 2.12e-12 70.5
Msa1229050 AT2G31400.1 22.360 322 209 9 183 493 411 702 6.65e-14 75.5
Msa1229050 AT2G31400.1 20.782 409 315 5 117 521 202 605 2.24e-12 70.5
Msa1229050 AT1G63150.2 24.354 271 198 5 180 447 152 418 2.27e-13 73.2
Msa1229050 AT1G63150.1 24.354 271 198 5 180 447 152 418 2.27e-13 73.2
Msa1229050 AT2G32630.1 19.294 425 298 7 122 508 200 617 4.49e-13 72.4
Msa1229050 AT5G65560.1 23.396 265 199 4 183 446 395 656 5.05e-13 72.4
Msa1229050 AT5G65560.2 23.396 265 199 4 183 446 395 656 5.05e-13 72.4
Msa1229050 AT5G65560.3 23.396 265 199 4 183 446 395 656 5.05e-13 72.4
Msa1229050 AT5G46580.1 22.615 283 214 4 175 453 250 531 6.35e-13 72.0
Msa1229050 AT1G43010.1 27.706 231 146 6 84 307 41 257 8.33e-13 68.9
Msa1229050 AT1G63230.1 21.118 322 240 6 180 498 9 319 2.05e-12 68.9
Msa1229050 AT3G22670.1 20.087 229 176 4 105 330 197 421 2.31e-12 70.1
Msa1229050 AT1G51965.1 21.322 469 295 15 109 517 113 567 2.35e-12 70.1
Msa1229050 AT5G25630.1 27.072 181 125 4 180 358 259 434 3.17e-12 69.7
Msa1229050 AT5G25630.3 27.072 181 125 4 180 358 208 383 3.30e-12 69.3
Msa1229050 AT4G11690.2 20.482 332 259 2 178 509 203 529 3.38e-12 69.3
Msa1229050 AT4G11690.1 20.482 332 259 2 178 509 230 556 3.41e-12 69.3
Msa1229050 AT1G20300.1 19.938 321 235 5 123 433 163 471 3.80e-12 69.3
Msa1229050 AT1G62590.2 24.277 346 241 7 117 452 231 565 3.95e-12 69.3
Msa1229050 AT1G62590.1 24.277 346 241 7 117 452 231 565 3.95e-12 69.3
Msa1229050 AT1G74900.1 22.096 353 256 9 110 452 52 395 4.01e-12 68.9
Msa1229050 AT2G35030.1 19.849 398 272 12 116 501 4 366 5.94e-12 68.9
Msa1229050 AT2G15630.1 20.728 357 247 5 183 507 262 614 6.15e-12 68.9
Msa1229050 AT1G63330.3 24.233 326 226 8 139 452 174 490 7.95e-12 68.2
Msa1229050 AT1G63330.1 24.233 326 226 8 139 452 174 490 7.95e-12 68.2
Msa1229050 AT1G63330.2 24.233 326 226 8 139 452 174 490 7.95e-12 68.2
Msa1229050 AT4G19890.1 23.750 320 224 7 183 494 324 631 8.31e-12 68.6
Msa1229050 AT1G10910.2 19.597 347 238 5 131 440 118 460 1.15e-11 67.8
Msa1229050 AT1G10910.1 19.597 347 238 5 131 440 118 460 1.18e-11 67.8
Msa1229050 AT5G04810.1 26.344 186 134 2 159 342 670 854 1.69e-11 67.8
Msa1229050 AT1G62910.1 21.324 408 304 7 117 518 229 625 1.73e-11 67.4
Msa1229050 AT1G62670.2 22.727 352 224 10 183 495 153 495 2.46e-11 67.0
Msa1229050 AT1G62670.1 22.727 352 224 10 183 495 153 495 2.46e-11 67.0
Msa1229050 AT4G31850.1 20.764 419 315 6 40 452 226 633 2.84e-11 67.0
Msa1229050 AT4G31850.1 21.184 321 240 6 180 496 292 603 3.34e-11 67.0
Msa1229050 AT5G16640.1 22.059 340 254 6 116 450 86 419 2.99e-11 66.2
Msa1229050 AT4G20090.2 21.569 357 266 7 127 476 164 513 3.32e-11 66.6
Msa1229050 AT4G20090.2 22.277 202 156 1 183 384 329 529 8.49e-11 65.1
Msa1229050 AT4G20090.1 21.569 357 266 7 127 476 164 513 3.32e-11 66.6
Msa1229050 AT4G20090.1 22.277 202 156 1 183 384 329 529 8.49e-11 65.1
Msa1229050 AT5G48730.1 22.455 334 243 7 150 474 149 475 3.44e-11 66.2
Msa1229050 AT3G09060.2 18.182 363 281 6 182 538 115 467 3.98e-11 66.2
Msa1229050 AT3G09060.1 18.182 363 281 6 182 538 115 467 3.98e-11 66.2
Msa1229050 AT5G42310.1 22.393 326 238 7 180 500 373 688 4.78e-11 66.2
Msa1229050 AT4G39620.2 22.289 332 241 10 117 437 103 428 5.54e-11 65.5
Msa1229050 AT5G39980.1 19.022 368 287 5 180 546 259 616 6.37e-11 65.5
Msa1229050 AT4G39620.1 22.289 332 241 10 117 437 103 428 8.21e-11 65.1
Msa1229050 AT5G61990.1 20.385 260 201 3 178 433 554 811 8.56e-11 65.5
Msa1229050 AT1G63070.1 18.873 355 274 4 172 518 235 583 9.29e-11 65.1

Find 111 sgRNAs with CRISPR-Local

Find 272 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATGACTTGTGTCTTTAAATT+TGG 0.114196 8_2:+4669296 Msa1229050:CDS
TATAGCATTCCATTTCAAAT+TGG 0.213868 8_2:-4665796 None:intergenic
AGCTCCCATGTAGTCGAATT+TGG 0.237732 8_2:-4668523 None:intergenic
ATCCTACCATTATTCCTAAT+TGG 0.237984 8_2:+4668089 Msa1229050:CDS
CGACTTTATCATAATCCTTA+AGG 0.296327 8_2:-4667934 None:intergenic
TCGAAGTAATGAGGAGTAAA+AGG 0.304308 8_2:+4667861 Msa1229050:CDS
TTCTGAGGCGCTTTCGTGCT+TGG 0.317772 8_2:+4668579 Msa1229050:CDS
GTACTAAATGTACTCCAATT+AGG 0.322076 8_2:-4668103 None:intergenic
AAAGGGAAAGAGAAACGTTT+TGG 0.332422 8_2:-4665623 None:intergenic
GAAACCCAAGCCAATAAAAT+TGG 0.333547 8_2:+4668636 Msa1229050:CDS
AAAACATGATAGAGCTCTTC+AGG 0.338362 8_2:+4665950 Msa1229050:CDS
TGTACTCCAATTAGGAATAA+TGG 0.340858 8_2:-4668095 None:intergenic
GATGATGGAGAATCCTGAAT+TGG 0.341809 8_2:+4665833 Msa1229050:CDS
TTTCGTCTTGACAAAGCTTA+AGG 0.346882 8_2:-4668666 None:intergenic
CATTATCTCCTGAGTTTGTA+TGG 0.359485 8_2:+4668226 Msa1229050:CDS
CTGCTTCTGTGTTGAATCAA+TGG 0.369186 8_2:+4665859 Msa1229050:CDS
AAGGCCGAGGGTAGAATATT+AGG 0.374501 8_2:+4668184 Msa1229050:CDS
ACTGACAGTGGAATTGGAAT+TGG 0.384505 8_2:+4665696 Msa1229050:CDS
ATAGCATTCCATTTCAAATT+GGG 0.388781 8_2:-4665795 None:intergenic
ATTGAGTTGTTGAAGAAGTC+GGG 0.392725 8_2:+4668715 Msa1229050:CDS
TATTTCTTCTCTCGTAAGAA+TGG 0.397940 8_2:+4668339 Msa1229050:CDS
GGGTCATATTGCTGAAAATA+GGG 0.397978 8_2:+4668555 Msa1229050:CDS
ATATATATACATATAATATC+TGG 0.400262 8_2:+4667999 Msa1229050:CDS
AGGGTCATATTGCTGAAAAT+AGG 0.400702 8_2:+4668554 Msa1229050:CDS
CCCTCTGATGATTCTAGAAT+AGG 0.408766 8_2:+4668412 Msa1229050:CDS
AGAGAAAACCCAATTTGAAA+TGG 0.410586 8_2:+4665787 Msa1229050:CDS
CCGCATACGCTTCGTCGTTA+TGG 0.412804 8_2:-4668741 None:intergenic
AACTCTGCAAAGTTCCCATT+TGG 0.415800 8_2:-4665904 None:intergenic
CAGAAAAGCTTTACGAGGAA+TGG 0.415843 8_2:+4668377 Msa1229050:CDS
GTTTCCAAGTATACCTAACT+TGG 0.418407 8_2:+4668303 Msa1229050:CDS
AAGGAAGGAATCTCACCAAA+TGG 0.426265 8_2:+4665889 Msa1229050:CDS
CACATTGACCCTTTGTTTGA+AGG 0.426662 8_2:-4665667 None:intergenic
ACAGCATGGTATCCCAAGTT+AGG 0.429036 8_2:-4668316 None:intergenic
AAGAAGATTGAATTTGAAGA+GGG 0.436849 8_2:-4665593 None:intergenic
TTAAAAGACAAGAGAACATA+TGG 0.437458 8_2:+4667782 Msa1229050:CDS
AATTGAGTTGTTGAAGAAGT+CGG 0.440073 8_2:+4668714 Msa1229050:CDS
ATTCTTTCAATCCTGTTTGA+CGG 0.440996 8_2:-4668784 None:intergenic
GAAGAGTATGAGAATAAGAA+AGG 0.442055 8_2:-4665641 None:intergenic
ACGAGGAATGGGTTTCTGTA+AGG 0.443573 8_2:+4668389 Msa1229050:CDS
TGCTTCTGTGTTGAATCAAT+GGG 0.447177 8_2:+4665860 Msa1229050:CDS
GCAAAGATGAACCGTCAAAC+AGG 0.449173 8_2:+4668773 Msa1229050:CDS
CGACAAGTGATTCAAAGAGT+TGG 0.450702 8_2:+4668614 Msa1229050:CDS
ATCTCAAAGATTCACAGTTA+TGG 0.451129 8_2:+4665750 Msa1229050:CDS
AGAGTCATCATTAAATTAAA+CGG 0.454778 8_2:-4667902 None:intergenic
CGTAAGAATGGACGATATTG+AGG 0.465330 8_2:+4668351 Msa1229050:CDS
ATACAAGAGGATTTCGATGA+TGG 0.469256 8_2:+4665818 Msa1229050:CDS
AGAAAAGCTTTACGAGGAAT+GGG 0.471834 8_2:+4668378 Msa1229050:CDS
TCAAGGATCAATAGAGAAGA+TGG 0.473452 8_2:+4668039 Msa1229050:CDS
ACAGAAGCAGAACCCAATTC+AGG 0.475894 8_2:-4665846 None:intergenic
TATCCCAAGTTAGGTATACT+TGG 0.477102 8_2:-4668307 None:intergenic
GGCTGCAATGTATATTAAGA+TGG 0.477129 8_2:+4668129 Msa1229050:CDS
GATCTAATCGCAAGAGTTCA+CGG 0.482500 8_2:+4667716 Msa1229050:CDS
GACTCTGTACATGAACCTTA+AGG 0.486004 8_2:+4667919 Msa1229050:CDS
ATGATGGAGAATCCTGAATT+GGG 0.490611 8_2:+4665834 Msa1229050:CDS
AATATCTGGTTGTCCTCTTG+TGG 0.497939 8_2:+4668013 Msa1229050:CDS
ATAAGCTGGTATCTTAAGAA+AGG 0.498356 8_2:+4668445 Msa1229050:CDS
TTATGATAAAGTCGATATGT+TGG 0.499930 8_2:+4667943 Msa1229050:CDS
GCAGATATTCTAAACCTCTC+TGG 0.506381 8_2:-4667671 None:intergenic
TTGTCCTCTTGTGGATCTCA+AGG 0.509360 8_2:+4668022 Msa1229050:CDS
ACATTGACCCTTTGTTTGAA+GGG 0.509737 8_2:-4665666 None:intergenic
TAGGAAACCTTCTCATAAGC+TGG 0.515507 8_2:+4668431 Msa1229050:CDS
CACGGAGTTTCTAGTGCAGA+AGG 0.519926 8_2:+4667734 Msa1229050:CDS
CTTCTGATGCATGTGATATG+AGG 0.519967 8_2:+4669252 Msa1229050:CDS
AAGAGTATGAGAATAAGAAA+GGG 0.530885 8_2:-4665640 None:intergenic
GACAGTGGAATTGGAATTGG+AGG 0.533917 8_2:+4665699 Msa1229050:CDS
TACATGGGAGCTTCTTTCTG+AGG 0.536998 8_2:+4668534 Msa1229050:CDS
GATGTCCAAATTCGACTACA+TGG 0.539568 8_2:+4668518 Msa1229050:CDS
CAACACTTCCATACAAACTC+AGG 0.539796 8_2:-4668234 None:intergenic
CTGAGTTTGTATGGAAGTGT+TGG 0.541520 8_2:+4668235 Msa1229050:CDS
TTATCTTCAACAAACAACAT+TGG 0.542696 8_2:+4665529 None:intergenic
AGGAAGGAATCTCACCAAAT+GGG 0.547368 8_2:+4665890 Msa1229050:CDS
TTCTTTAAGCATATGAGTGA+AGG 0.550405 8_2:+4668490 Msa1229050:CDS
TGCTGAAAATAGGGTTTCTG+AGG 0.551041 8_2:+4668564 Msa1229050:CDS
CATTGACCCTTTGTTTGAAG+GGG 0.551949 8_2:-4665665 None:intergenic
AGGACGAAGTTTACCGCGTG+TGG 0.552160 8_2:+4668263 Msa1229050:CDS
GTAAGAATGGACGATATTGA+GGG 0.554710 8_2:+4668352 Msa1229050:CDS
GTATGGAAGTGTTGGAAACA+AGG 0.554809 8_2:+4668243 Msa1229050:CDS
ATCAATGGGAGAATGAAGGA+AGG 0.564566 8_2:+4665874 Msa1229050:CDS
AAACTCAGGAGATAATGAAA+AGG 0.565979 8_2:-4668220 None:intergenic
TCGACCTAATATTCTACCCT+CGG 0.566861 8_2:-4668188 None:intergenic
AAAGAAGATTGAATTTGAAG+AGG 0.567795 8_2:-4665594 None:intergenic
AATCTTTACTCGAAGTAATG+AGG 0.567840 8_2:+4667852 Msa1229050:CDS
TCATTCTAGATCAAGAGAAA+AGG 0.571003 8_2:+4667826 Msa1229050:CDS
CAACAACTCAATTAAAACCT+CGG 0.574815 8_2:-4668704 None:intergenic
TAAGATACCAGCTTATGAGA+AGG 0.579937 8_2:-4668438 None:intergenic
TTGGAGTACATTTAGTACAA+TGG 0.583230 8_2:+4668108 Msa1229050:CDS
TTTCCAAGTATACCTAACTT+GGG 0.589423 8_2:+4668304 Msa1229050:CDS
TTTAAGCATATGAGTGAAGG+TGG 0.593303 8_2:+4668493 Msa1229050:CDS
ACATGGGAGCTTCTTTCTGA+GGG 0.601564 8_2:+4668535 Msa1229050:CDS
AATGAACATATGCATTCAAG+AGG 0.604245 8_2:-4667808 None:intergenic
TCCTATTCTAGAATCATCAG+AGG 0.618071 8_2:-4668413 None:intergenic
ATAGAAAACATACACAAGGA+TGG 0.618846 8_2:-4669275 None:intergenic
ATTGACCCTTTGTTTGAAGG+GGG 0.620972 8_2:-4665664 None:intergenic
TTGAATCAATGGGAGAATGA+AGG 0.623335 8_2:+4665870 Msa1229050:CDS
AATGGAATGCTATATACAAG+AGG 0.631419 8_2:+4665805 Msa1229050:CDS
AGAAGATTGAATTTGAAGAG+GGG 0.639018 8_2:-4665592 None:intergenic
TTGACCCTTTGTTTGAAGGG+GGG 0.640200 8_2:-4665663 None:intergenic
TTGTCAAGACGAAAACGACA+TGG 0.642627 8_2:+4668675 Msa1229050:CDS
CCTATTCTAGAATCATCAGA+GGG 0.647606 8_2:-4668412 None:intergenic
ATGAACATATGCATTCAAGA+GGG 0.654487 8_2:-4667807 None:intergenic
AAGCATATGAGTGAAGGTGG+TGG 0.657423 8_2:+4668496 Msa1229050:CDS
ATGTCCAAATTCGACTACAT+GGG 0.669288 8_2:+4668519 Msa1229050:CDS
TGATCCTTGAGATCCACAAG+AGG 0.674298 8_2:-4668026 None:intergenic
CGAGAGAAGAAATAACAGCA+TGG 0.674858 8_2:-4668330 None:intergenic
CTCCAATTAGGAATAATGGT+AGG 0.679561 8_2:-4668091 None:intergenic
TAAGAATGGACGATATTGAG+GGG 0.688947 8_2:+4668353 Msa1229050:CDS
AAGAATGGACGATATTGAGG+GGG 0.691386 8_2:+4668354 Msa1229050:CDS
ATTTATAGTTATTCCACACG+CGG 0.697764 8_2:-4668276 None:intergenic
AGTCATAGAAAACATACACA+AGG 0.711577 8_2:-4669279 None:intergenic
AAACGACATGGAGAGCGCCG+AGG 0.711901 8_2:+4668687 Msa1229050:CDS
GGATGAACAATAGACCAGAG+AGG 0.722678 8_2:+4667657 Msa1229050:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATATATACATATAATATC+TGG + chr8_2:4667999-4668018 Msa1229050:CDS 10.0%
!! TCATTATATGTTATTCTATA+TGG + chr8_2:4665989-4666008 Msa1229050:intron 15.0%
!! AAACAAAAAATATGATTGAA+AGG - chr8_2:4666957-4666976 None:intergenic 15.0%
!! AAAAAATTTATATCAGTTTG+AGG - chr8_2:4667211-4667230 None:intergenic 15.0%
!!! AAGAATTGTGTTTTTTAATC+AGG - chr8_2:4666552-4666571 None:intergenic 20.0%
!!! TAGAATTCTTGTTTATTTTC+CGG - chr8_2:4666607-4666626 None:intergenic 20.0%
!! CACACACAAAAAAAAAAAAA+AGG + chr8_2:4666773-4666792 Msa1229050:intron 20.0%
!!! GTTAAAGGTTTTTGATTTTT+TGG - chr8_2:4667012-4667031 None:intergenic 20.0%
!! GTAGTAAAAGAAATACATAA+CGG + chr8_2:4667178-4667197 Msa1229050:intron 20.0%
!!! GGAAATTTTCAATATATTCA+GGG - chr8_2:4667350-4667369 None:intergenic 20.0%
!!! AGGAAATTTTCAATATATTC+AGG - chr8_2:4667351-4667370 None:intergenic 20.0%
!! TGATATTAAGACATTATTTG+AGG - chr8_2:4667579-4667598 None:intergenic 20.0%
!! AGAGTCATCATTAAATTAAA+CGG - chr8_2:4667905-4667924 None:intergenic 20.0%
! AAGAAGATTGAATTTGAAGA+GGG - chr8_2:4665596-4665615 None:intergenic 25.0%
! AAAGAAGATTGAATTTGAAG+AGG - chr8_2:4665597-4665616 None:intergenic 25.0%
!! AAGAGTATGAGAATAAGAAA+GGG - chr8_2:4665643-4665662 None:intergenic 25.0%
! ATAGCATTCCATTTCAAATT+GGG - chr8_2:4665798-4665817 None:intergenic 25.0%
! TATAGCATTCCATTTCAAAT+TGG - chr8_2:4665799-4665818 None:intergenic 25.0%
!!! TGTGTTTTTTAATCAGGAAA+TGG - chr8_2:4666546-4666565 None:intergenic 25.0%
!!! AGAATTCTTGTTTATTTTCC+GGG - chr8_2:4666606-4666625 None:intergenic 25.0%
! TTAAGTTTAAATGTCTCTGT+TGG - chr8_2:4666675-4666694 None:intergenic 25.0%
! AAATCATAATGTATCATGAG+AGG - chr8_2:4666840-4666859 None:intergenic 25.0%
! TTTGGGGATAGAAATTATTA+AGG - chr8_2:4666929-4666948 None:intergenic 25.0%
!! ATGATTGAAAGGTATGATTT+GGG - chr8_2:4666946-4666965 None:intergenic 25.0%
!! TATGATTGAAAGGTATGATT+TGG - chr8_2:4666947-4666966 None:intergenic 25.0%
! TTAAAACTTGTAGTGGTTAA+AGG - chr8_2:4667027-4667046 None:intergenic 25.0%
!! ACACAATTTAAAACTTGTAG+TGG - chr8_2:4667034-4667053 None:intergenic 25.0%
!! ACTGACCAATACAATTTTTA+GGG - chr8_2:4667084-4667103 None:intergenic 25.0%
!! ATTTCTTTTACTACGTTTCT+TGG - chr8_2:4667172-4667191 None:intergenic 25.0%
!! TAAAAGAAATACATAACGGA+CGG + chr8_2:4667182-4667201 Msa1229050:intron 25.0%
!! ACACCAACGTTAAAATTTTA+TGG + chr8_2:4667260-4667279 Msa1229050:intron 25.0%
!! CACCAACGTTAAAATTTTAT+GGG + chr8_2:4667261-4667280 Msa1229050:intron 25.0%
!! ACCAACGTTAAAATTTTATG+GGG + chr8_2:4667262-4667281 Msa1229050:intron 25.0%
!!! TTTTTTTTTACCAACAGTCT+AGG - chr8_2:4667371-4667390 None:intergenic 25.0%
! TACTTTCAAAAAATCATTCG+TGG - chr8_2:4667603-4667622 None:intergenic 25.0%
!!! TGTCTTTTAAATCATTGGTT+AGG - chr8_2:4667772-4667791 None:intergenic 25.0%
!! TCTCTTGTCTTTTAAATCAT+TGG - chr8_2:4667777-4667796 None:intergenic 25.0%
! TTAAAAGACAAGAGAACATA+TGG + chr8_2:4667782-4667801 Msa1229050:CDS 25.0%
! TTATGATAAAGTCGATATGT+TGG + chr8_2:4667943-4667962 Msa1229050:CDS 25.0%
! TTGAATGTAATAACATGTGT+TGG - chr8_2:4668963-4668982 None:intergenic 25.0%
!!! TCAAATTTTGTTTTGACTTG+TGG + chr8_2:4669085-4669104 Msa1229050:intron 25.0%
!!! AAAATGAGTTGATAGAACAT+AGG + chr8_2:4669108-4669127 Msa1229050:intron 25.0%
!!! AAATGAGTTGATAGAACATA+GGG + chr8_2:4669109-4669128 Msa1229050:intron 25.0%
!! TTCTTTTTCTCAAGCTTTTA+TGG + chr8_2:4669147-4669166 Msa1229050:intron 25.0%
!! ATGACTTGTGTCTTTAAATT+TGG + chr8_2:4669296-4669315 Msa1229050:CDS 25.0%
AGAAGATTGAATTTGAAGAG+GGG - chr8_2:4665595-4665614 None:intergenic 30.0%
! GAAGAGTATGAGAATAAGAA+AGG - chr8_2:4665644-4665663 None:intergenic 30.0%
ATCTCAAAGATTCACAGTTA+TGG + chr8_2:4665750-4665769 Msa1229050:CDS 30.0%
AGAGAAAACCCAATTTGAAA+TGG + chr8_2:4665787-4665806 Msa1229050:CDS 30.0%
AATGGAATGCTATATACAAG+AGG + chr8_2:4665805-4665824 Msa1229050:CDS 30.0%
TTGCTTCATAATGTTAAGGA+GGG - chr8_2:4666139-4666158 None:intergenic 30.0%
GTTCAATTCAATCTGTTGTT+CGG - chr8_2:4666176-4666195 None:intergenic 30.0%
TCCAAAAGAGTAAGAATGAA+CGG + chr8_2:4666196-4666215 Msa1229050:intron 30.0%
!! GAATTCTTGTTTATTTTCCG+GGG - chr8_2:4666605-4666624 None:intergenic 30.0%
CTAGAAGACATTAATGCAAT+AGG + chr8_2:4666624-4666643 Msa1229050:intron 30.0%
AATATATTCAGCTGCACAAT+TGG - chr8_2:4666722-4666741 None:intergenic 30.0%
AATTGTGATAGCTAAAGTGT+CGG - chr8_2:4666899-4666918 None:intergenic 30.0%
! TGATTGAAAGGTATGATTTG+GGG - chr8_2:4666945-4666964 None:intergenic 30.0%
ATCCACCCTAAAAATTGTAT+TGG + chr8_2:4667076-4667095 Msa1229050:intron 30.0%
! CACTGACCAATACAATTTTT+AGG - chr8_2:4667085-4667104 None:intergenic 30.0%
! CTAAAAATTGTATTGGTCAG+TGG + chr8_2:4667083-4667102 Msa1229050:intron 30.0%
TTGGATTTCATAGATGACTA+AGG - chr8_2:4667153-4667172 None:intergenic 30.0%
TTGAAAATTTCCTAGACTGT+TGG + chr8_2:4667358-4667377 Msa1229050:intron 30.0%
ACATGTGGATAGTATAAGAA+TGG - chr8_2:4667414-4667433 None:intergenic 30.0%
TTCCGTTTAGTAGAATCATA+GGG - chr8_2:4667491-4667510 None:intergenic 30.0%
ATTCCGTTTAGTAGAATCAT+AGG - chr8_2:4667492-4667511 None:intergenic 30.0%
! TTAAGACATTATTTGAGGCA+AGG - chr8_2:4667574-4667593 None:intergenic 30.0%
! ATGTTTTGCAGGTATATGAT+TGG + chr8_2:4667636-4667655 Msa1229050:intron 30.0%
ATGAACATATGCATTCAAGA+GGG - chr8_2:4667810-4667829 None:intergenic 30.0%
AATGAACATATGCATTCAAG+AGG - chr8_2:4667811-4667830 None:intergenic 30.0%
TCATTCTAGATCAAGAGAAA+AGG + chr8_2:4667826-4667845 Msa1229050:CDS 30.0%
AATCTTTACTCGAAGTAATG+AGG + chr8_2:4667852-4667871 Msa1229050:CDS 30.0%
CGACTTTATCATAATCCTTA+AGG - chr8_2:4667937-4667956 None:intergenic 30.0%
! AGTTTTTGAACAGATGTCAA+AGG + chr8_2:4668066-4668085 Msa1229050:CDS 30.0%
ATCCTACCATTATTCCTAAT+TGG + chr8_2:4668089-4668108 Msa1229050:CDS 30.0%
TGTACTCCAATTAGGAATAA+TGG - chr8_2:4668098-4668117 None:intergenic 30.0%
GTACTAAATGTACTCCAATT+AGG - chr8_2:4668106-4668125 None:intergenic 30.0%
TTGGAGTACATTTAGTACAA+TGG + chr8_2:4668108-4668127 Msa1229050:CDS 30.0%
AAACTCAGGAGATAATGAAA+AGG - chr8_2:4668223-4668242 None:intergenic 30.0%
ATTTATAGTTATTCCACACG+CGG - chr8_2:4668279-4668298 None:intergenic 30.0%
TTTCCAAGTATACCTAACTT+GGG + chr8_2:4668304-4668323 Msa1229050:CDS 30.0%
TATTTCTTCTCTCGTAAGAA+TGG + chr8_2:4668339-4668358 Msa1229050:CDS 30.0%
ATAAGCTGGTATCTTAAGAA+AGG + chr8_2:4668445-4668464 Msa1229050:CDS 30.0%
TTCTTTAAGCATATGAGTGA+AGG + chr8_2:4668490-4668509 Msa1229050:CDS 30.0%
CAACAACTCAATTAAAACCT+CGG - chr8_2:4668707-4668726 None:intergenic 30.0%
! AATTGAGTTGTTGAAGAAGT+CGG + chr8_2:4668714-4668733 Msa1229050:CDS 30.0%
ATTCTTTCAATCCTGTTTGA+CGG - chr8_2:4668787-4668806 None:intergenic 30.0%
ATCAAAAGCTACTATCTACT+TGG - chr8_2:4668864-4668883 None:intergenic 30.0%
! ACACCTTCAATTTGAAATGT+AGG - chr8_2:4668899-4668918 None:intergenic 30.0%
!! AATGAGTTGATAGAACATAG+GGG + chr8_2:4669110-4669129 Msa1229050:intron 30.0%
CTTATCACAACAGTTATGAA+GGG + chr8_2:4669170-4669189 Msa1229050:intron 30.0%
ATAGAAAACATACACAAGGA+TGG - chr8_2:4669278-4669297 None:intergenic 30.0%
AGTCATAGAAAACATACACA+AGG - chr8_2:4669282-4669301 None:intergenic 30.0%
! AAAGGGAAAGAGAAACGTTT+TGG - chr8_2:4665626-4665645 None:intergenic 35.0%
! ACATTGACCCTTTGTTTGAA+GGG - chr8_2:4665669-4665688 None:intergenic 35.0%
! AATGTGTTTTACACTGACAG+TGG + chr8_2:4665684-4665703 Msa1229050:CDS 35.0%
! TTTTACACTGACAGTGGAAT+TGG + chr8_2:4665690-4665709 Msa1229050:CDS 35.0%
ATACAAGAGGATTTCGATGA+TGG + chr8_2:4665818-4665837 Msa1229050:CDS 35.0%
! ATGATGGAGAATCCTGAATT+GGG + chr8_2:4665834-4665853 Msa1229050:CDS 35.0%
TGCTTCTGTGTTGAATCAAT+GGG + chr8_2:4665860-4665879 Msa1229050:CDS 35.0%
TTGAATCAATGGGAGAATGA+AGG + chr8_2:4665870-4665889 Msa1229050:CDS 35.0%
AAAACATGATAGAGCTCTTC+AGG + chr8_2:4665950-4665969 Msa1229050:CDS 35.0%
ATGGTTAAAACAACTGCAGT+AGG + chr8_2:4666008-4666027 Msa1229050:intron 35.0%
!!! CCTAGCTTTTTTGGTTTTTG+CGG + chr8_2:4666031-4666050 Msa1229050:intron 35.0%
!!! TGCTAGTTTTCGCTGTTTTA+GGG + chr8_2:4666091-4666110 Msa1229050:intron 35.0%
TGCTTCATAATGTTAAGGAG+GGG - chr8_2:4666138-4666157 None:intergenic 35.0%
GTTGCTTCATAATGTTAAGG+AGG - chr8_2:4666140-4666159 None:intergenic 35.0%
! GCTGTTGCTTCATAATGTTA+AGG - chr8_2:4666143-4666162 None:intergenic 35.0%
! ACCGTTCATTCTTACTCTTT+TGG - chr8_2:4666200-4666219 None:intergenic 35.0%
! TAACATGGTAGTGTATTGGA+TGG - chr8_2:4666228-4666247 None:intergenic 35.0%
TCTTTGGTACGATAGAGATT+GGG - chr8_2:4666282-4666301 None:intergenic 35.0%
TGTTGGACCATTCCATAATA+CGG + chr8_2:4666311-4666330 Msa1229050:intron 35.0%
TTGGTCATGATATAGTTCTC+AGG - chr8_2:4666578-4666597 None:intergenic 35.0%
GACATTAATGCAATAGGACA+TGG + chr8_2:4666630-4666649 Msa1229050:intron 35.0%
TTGCCAAATAGATGACACTT+TGG + chr8_2:4666858-4666877 Msa1229050:intron 35.0%
! TAGCCAAAGTGTCATCTATT+TGG - chr8_2:4666864-4666883 None:intergenic 35.0%
TTTGGACACAGATTGAACAA+TGG - chr8_2:4666994-4667013 None:intergenic 35.0%
!! GACCAATACAATTTTTAGGG+TGG - chr8_2:4667081-4667100 None:intergenic 35.0%
!! GCCCCATAAAATTTTAACGT+TGG - chr8_2:4667266-4667285 None:intergenic 35.0%
! ACGTTAAAATTTTATGGGGC+CGG + chr8_2:4667266-4667285 Msa1229050:intron 35.0%
TTACTGACAGTTTGCAAATC+TGG - chr8_2:4667442-4667461 None:intergenic 35.0%
TAGTAGAATCATAGGGGAAA+TGG - chr8_2:4667484-4667503 None:intergenic 35.0%
TCCCCTATGATTCTACTAAA+CGG + chr8_2:4667486-4667505 Msa1229050:intron 35.0%
TCCGTTTAGTAGAATCATAG+GGG - chr8_2:4667490-4667509 None:intergenic 35.0%
CATACTTTCATGCATTCCAA+AGG - chr8_2:4667519-4667538 None:intergenic 35.0%
! GACTAATGAACATGTTTTGC+AGG + chr8_2:4667625-4667644 Msa1229050:intron 35.0%
TCGAAGTAATGAGGAGTAAA+AGG + chr8_2:4667861-4667880 Msa1229050:CDS 35.0%
TCAAGGATCAATAGAGAAGA+TGG + chr8_2:4668039-4668058 Msa1229050:CDS 35.0%
CTCCAATTAGGAATAATGGT+AGG - chr8_2:4668094-4668113 None:intergenic 35.0%
GGCTGCAATGTATATTAAGA+TGG + chr8_2:4668129-4668148 Msa1229050:CDS 35.0%
!! AGCACAAGAGTGCTTAAAAA+AGG + chr8_2:4668165-4668184 Msa1229050:CDS 35.0%
CATTATCTCCTGAGTTTGTA+TGG + chr8_2:4668226-4668245 Msa1229050:CDS 35.0%
GTTTCCAAGTATACCTAACT+TGG + chr8_2:4668303-4668322 Msa1229050:CDS 35.0%
TATCCCAAGTTAGGTATACT+TGG - chr8_2:4668310-4668329 None:intergenic 35.0%
GTAAGAATGGACGATATTGA+GGG + chr8_2:4668352-4668371 Msa1229050:CDS 35.0%
TAAGAATGGACGATATTGAG+GGG + chr8_2:4668353-4668372 Msa1229050:CDS 35.0%
AGAAAAGCTTTACGAGGAAT+GGG + chr8_2:4668378-4668397 Msa1229050:CDS 35.0%
!! CCTATTCTAGAATCATCAGA+GGG - chr8_2:4668415-4668434 None:intergenic 35.0%
!! TCCTATTCTAGAATCATCAG+AGG - chr8_2:4668416-4668435 None:intergenic 35.0%
TAAGATACCAGCTTATGAGA+AGG - chr8_2:4668441-4668460 None:intergenic 35.0%
TTTAAGCATATGAGTGAAGG+TGG + chr8_2:4668493-4668512 Msa1229050:CDS 35.0%
ATGTCCAAATTCGACTACAT+GGG + chr8_2:4668519-4668538 Msa1229050:CDS 35.0%
AGGGTCATATTGCTGAAAAT+AGG + chr8_2:4668554-4668573 Msa1229050:CDS 35.0%
GGGTCATATTGCTGAAAATA+GGG + chr8_2:4668555-4668574 Msa1229050:CDS 35.0%
GAAACCCAAGCCAATAAAAT+TGG + chr8_2:4668636-4668655 Msa1229050:CDS 35.0%
! AGGAATGCAGCCAATTTTAT+TGG - chr8_2:4668649-4668668 None:intergenic 35.0%
TTTCGTCTTGACAAAGCTTA+AGG - chr8_2:4668669-4668688 None:intergenic 35.0%
! ATTGAGTTGTTGAAGAAGTC+GGG + chr8_2:4668715-4668734 Msa1229050:CDS 35.0%
! TTGAAATGTAGGTGCTACAT+AGG - chr8_2:4668888-4668907 None:intergenic 35.0%
GCACCTACATTTCAAATTGA+AGG + chr8_2:4668893-4668912 Msa1229050:intron 35.0%
! CATTTCAAATTGAAGGTGTG+TGG + chr8_2:4668900-4668919 Msa1229050:intron 35.0%
TGTTGGTGTAATGTCAATGT+TGG - chr8_2:4668946-4668965 None:intergenic 35.0%
!! ATGAGTTGATAGAACATAGG+GGG + chr8_2:4669111-4669130 Msa1229050:intron 35.0%
GCTTATCACAACAGTTATGA+AGG + chr8_2:4669169-4669188 Msa1229050:intron 35.0%
! GACTGATGTTTTGCTCTAAT+AGG + chr8_2:4669221-4669240 Msa1229050:intron 35.0%
! ATTGACCCTTTGTTTGAAGG+GGG - chr8_2:4665667-4665686 None:intergenic 40.0%
! CATTGACCCTTTGTTTGAAG+GGG - chr8_2:4665668-4665687 None:intergenic 40.0%
! CACATTGACCCTTTGTTTGA+AGG - chr8_2:4665670-4665689 None:intergenic 40.0%
ACTGACAGTGGAATTGGAAT+TGG + chr8_2:4665696-4665715 Msa1229050:CDS 40.0%
!! GATGATGGAGAATCCTGAAT+TGG + chr8_2:4665833-4665852 Msa1229050:CDS 40.0%
CTGCTTCTGTGTTGAATCAA+TGG + chr8_2:4665859-4665878 Msa1229050:CDS 40.0%
ATCAATGGGAGAATGAAGGA+AGG + chr8_2:4665874-4665893 Msa1229050:CDS 40.0%
AAGGAAGGAATCTCACCAAA+TGG + chr8_2:4665889-4665908 Msa1229050:CDS 40.0%
AGGAAGGAATCTCACCAAAT+GGG + chr8_2:4665890-4665909 Msa1229050:CDS 40.0%
AACTCTGCAAAGTTCCCATT+TGG - chr8_2:4665907-4665926 None:intergenic 40.0%
CCGCAAAAACCAAAAAAGCT+AGG - chr8_2:4666034-4666053 None:intergenic 40.0%
!!! CTGCTAGTTTTCGCTGTTTT+AGG + chr8_2:4666090-4666109 Msa1229050:intron 40.0%
!!! GCTAGTTTTCGCTGTTTTAG+GGG + chr8_2:4666092-4666111 Msa1229050:intron 40.0%
!! GAGGGGGTATGTTTTTAACA+CGG - chr8_2:4666121-4666140 None:intergenic 40.0%
GCTTCATAATGTTAAGGAGG+GGG - chr8_2:4666137-4666156 None:intergenic 40.0%
! AGGCTAACATGGTAGTGTAT+TGG - chr8_2:4666232-4666251 None:intergenic 40.0%
GTCTTTGGTACGATAGAGAT+TGG - chr8_2:4666283-4666302 None:intergenic 40.0%
TCACCATCCGTATTATGGAA+TGG - chr8_2:4666321-4666340 None:intergenic 40.0%
GATGATCACCATCCGTATTA+TGG - chr8_2:4666326-4666345 None:intergenic 40.0%
! TCTATTTTCAGCAGGACAAC+AGG - chr8_2:4666468-4666487 None:intergenic 40.0%
! GTTTTTACTTTGGCTGATGG+AGG - chr8_2:4666515-4666534 None:intergenic 40.0%
!!! CCTGTTTTTACTTTGGCTGA+TGG - chr8_2:4666518-4666537 None:intergenic 40.0%
CCATCAGCCAAAGTAAAAAC+AGG + chr8_2:4666515-4666534 Msa1229050:intron 40.0%
!!! GGTTGCTCCTGTTTTTACTT+TGG - chr8_2:4666525-4666544 None:intergenic 40.0%
ATATCATGACCAAACTACCC+CGG + chr8_2:4666585-4666604 Msa1229050:intron 40.0%
!! GTTTATTTTCCGGGGTAGTT+TGG - chr8_2:4666597-4666616 None:intergenic 40.0%
! TTAACAGCTTTTGCAGTGAC+TGG + chr8_2:4666691-4666710 Msa1229050:intron 40.0%
! TTTGTGTGTGTGTTGCTGAT+TGG - chr8_2:4666763-4666782 None:intergenic 40.0%
! TAGGGTGGATTTCATAGTCT+TGG - chr8_2:4667066-4667085 None:intergenic 40.0%
TTAACGTTGGTGTGAGAACA+TGG - chr8_2:4667253-4667272 None:intergenic 40.0%
TACCACGTCAAAAGTTTAGC+CGG - chr8_2:4667288-4667307 None:intergenic 40.0%
! GCAAATCTGGAATTGACATG+TGG - chr8_2:4667429-4667448 None:intergenic 40.0%
ACTAAACGGAATGCTTCCTT+TGG + chr8_2:4667500-4667519 Msa1229050:intron 40.0%
GCAGATATTCTAAACCTCTC+TGG - chr8_2:4667674-4667693 None:intergenic 40.0%
GATCTAATCGCAAGAGTTCA+CGG + chr8_2:4667716-4667735 Msa1229050:CDS 40.0%
GACTCTGTACATGAACCTTA+AGG + chr8_2:4667919-4667938 Msa1229050:CDS 40.0%
AATATCTGGTTGTCCTCTTG+TGG + chr8_2:4668013-4668032 Msa1229050:CDS 40.0%
TCGACCTAATATTCTACCCT+CGG - chr8_2:4668191-4668210 None:intergenic 40.0%
CAACACTTCCATACAAACTC+AGG - chr8_2:4668237-4668256 None:intergenic 40.0%
! CTGAGTTTGTATGGAAGTGT+TGG + chr8_2:4668235-4668254 Msa1229050:CDS 40.0%
!! GTATGGAAGTGTTGGAAACA+AGG + chr8_2:4668243-4668262 Msa1229050:CDS 40.0%
CGAGAGAAGAAATAACAGCA+TGG - chr8_2:4668333-4668352 None:intergenic 40.0%
CGTAAGAATGGACGATATTG+AGG + chr8_2:4668351-4668370 Msa1229050:CDS 40.0%
AAGAATGGACGATATTGAGG+GGG + chr8_2:4668354-4668373 Msa1229050:CDS 40.0%
CAGAAAAGCTTTACGAGGAA+TGG + chr8_2:4668377-4668396 Msa1229050:CDS 40.0%
!! CCCTCTGATGATTCTAGAAT+AGG + chr8_2:4668412-4668431 Msa1229050:CDS 40.0%
TAGGAAACCTTCTCATAAGC+TGG + chr8_2:4668431-4668450 Msa1229050:CDS 40.0%
GATGTCCAAATTCGACTACA+TGG + chr8_2:4668518-4668537 Msa1229050:CDS 40.0%
TGCTGAAAATAGGGTTTCTG+AGG + chr8_2:4668564-4668583 Msa1229050:CDS 40.0%
CGACAAGTGATTCAAAGAGT+TGG + chr8_2:4668614-4668633 Msa1229050:CDS 40.0%
!!! GCAGCCAATTTTATTGGCTT+GGG - chr8_2:4668643-4668662 None:intergenic 40.0%
!! TGCAGCCAATTTTATTGGCT+TGG - chr8_2:4668644-4668663 None:intergenic 40.0%
TTGTCAAGACGAAAACGACA+TGG + chr8_2:4668675-4668694 Msa1229050:CDS 40.0%
CTAGGTTCTTATGCTAGGTT+AGG + chr8_2:4669193-4669212 Msa1229050:intron 40.0%
TCTTATGCTAGGTTAGGACA+TGG + chr8_2:4669199-4669218 Msa1229050:intron 40.0%
CTTCTGATGCATGTGATATG+AGG + chr8_2:4669252-4669271 Msa1229050:CDS 40.0%
TCTTCCCCCCTTCAAACAAA+GGG + chr8_2:4665659-4665678 Msa1229050:CDS 45.0%
! TTGACCCTTTGTTTGAAGGG+GGG - chr8_2:4665666-4665685 None:intergenic 45.0%
GACAGTGGAATTGGAATTGG+AGG + chr8_2:4665699-4665718 Msa1229050:CDS 45.0%
ACAGAAGCAGAACCCAATTC+AGG - chr8_2:4665849-4665868 None:intergenic 45.0%
!!! TGCAGTAGGCCTAGCTTTTT+TGG + chr8_2:4666022-4666041 Msa1229050:intron 45.0%
!!! TTGGTTTTTGCGGTGCTGTT+TGG + chr8_2:4666041-4666060 Msa1229050:intron 45.0%
CATCTACAGTGAGGCTAACA+TGG - chr8_2:4666243-4666262 None:intergenic 45.0%
CGTAGTTCACATCTACAGTG+AGG - chr8_2:4666252-4666271 None:intergenic 45.0%
TTGGTACGATAGAGATTGGG+AGG - chr8_2:4666279-4666298 None:intergenic 45.0%
ATGGAATGGTCCAACAACAG+TGG - chr8_2:4666307-4666326 None:intergenic 45.0%
GGACCATTCCATAATACGGA+TGG + chr8_2:4666315-4666334 Msa1229050:intron 45.0%
! AAGCCTTATCCCACTAAGTG+GGG - chr8_2:4666363-4666382 None:intergenic 45.0%
! CAAGCCTTATCCCACTAAGT+GGG - chr8_2:4666364-4666383 None:intergenic 45.0%
! GGAGGGATTCTATTTTCAGC+AGG - chr8_2:4666476-4666495 None:intergenic 45.0%
GGATGAACAATAGACCAGAG+AGG + chr8_2:4667657-4667676 Msa1229050:CDS 45.0%
TTGTCCTCTTGTGGATCTCA+AGG + chr8_2:4668022-4668041 Msa1229050:CDS 45.0%
TGATCCTTGAGATCCACAAG+AGG - chr8_2:4668029-4668048 None:intergenic 45.0%
! AGAGTGCTTAAAAAAGGCCG+AGG + chr8_2:4668171-4668190 Msa1229050:CDS 45.0%
GAGTGCTTAAAAAAGGCCGA+GGG + chr8_2:4668172-4668191 Msa1229050:CDS 45.0%
AAGGCCGAGGGTAGAATATT+AGG + chr8_2:4668184-4668203 Msa1229050:CDS 45.0%
ACAGCATGGTATCCCAAGTT+AGG - chr8_2:4668319-4668338 None:intergenic 45.0%
! ACGAGGAATGGGTTTCTGTA+AGG + chr8_2:4668389-4668408 Msa1229050:CDS 45.0%
AAGCATATGAGTGAAGGTGG+TGG + chr8_2:4668496-4668515 Msa1229050:CDS 45.0%
AGCTCCCATGTAGTCGAATT+TGG - chr8_2:4668526-4668545 None:intergenic 45.0%
TACATGGGAGCTTCTTTCTG+AGG + chr8_2:4668534-4668553 Msa1229050:CDS 45.0%
ACATGGGAGCTTCTTTCTGA+GGG + chr8_2:4668535-4668554 Msa1229050:CDS 45.0%
GCAAAGATGAACCGTCAAAC+AGG + chr8_2:4668773-4668792 Msa1229050:CDS 45.0%
GTGTGGTGTCTAACATGTGT+CGG + chr8_2:4668917-4668936 Msa1229050:intron 45.0%
CACAACAGTTATGAAGGGCT+AGG + chr8_2:4669175-4669194 Msa1229050:intron 45.0%
AAGGGCTAGGTTCTTATGCT+AGG + chr8_2:4669188-4669207 Msa1229050:intron 45.0%
!!! TATAAATTTTTTAATTCAAA+AGG + chr8_2:4667219-4667238 Msa1229050:intron 5.0%
CTCTTCCCCCCTTCAAACAA+AGG + chr8_2:4665658-4665677 Msa1229050:CDS 50.0%
ACCAAAGACGCCACTGTTGT+TGG + chr8_2:4666294-4666313 Msa1229050:intron 50.0%
TCCAACAACAGTGGCGTCTT+TGG - chr8_2:4666298-4666317 None:intergenic 50.0%
TGTTATCCGACCCCACTTAG+TGG + chr8_2:4666350-4666369 Msa1229050:intron 50.0%
GTTATCCGACCCCACTTAGT+GGG + chr8_2:4666351-4666370 Msa1229050:intron 50.0%
CTTATCCCACTAAGTGGGGT+CGG - chr8_2:4666359-4666378 None:intergenic 50.0%
! CCAAGCCTTATCCCACTAAG+TGG - chr8_2:4666365-4666384 None:intergenic 50.0%
CCACTTAGTGGGATAAGGCT+TGG + chr8_2:4666362-4666381 Msa1229050:intron 50.0%
CTCCACCAGAACTGCTTCAT+TGG + chr8_2:4666442-4666461 Msa1229050:intron 50.0%
ACAGGCCAATGAAGCAGTTC+TGG - chr8_2:4666450-4666469 None:intergenic 50.0%
! ACTTTGGCTGATGGAGGCAT+CGG - chr8_2:4666509-4666528 None:intergenic 50.0%
GTCAGTGGTGACTCAGTTGA+TGG + chr8_2:4667098-4667117 Msa1229050:intron 50.0%
ACGTTGGTGTGAGAACATGG+AGG - chr8_2:4667250-4667269 None:intergenic 50.0%
!! CACGGAGTTTCTAGTGCAGA+AGG + chr8_2:4667734-4667753 Msa1229050:CDS 50.0%
CCATAACGACGAAGCGTATG+CGG + chr8_2:4668741-4668760 Msa1229050:CDS 50.0%
TCCTCGATGTGTGTGTGTGT+GGG + chr8_2:4669043-4669062 Msa1229050:intron 50.0%
CGACCCCACTTAGTGGGATA+AGG + chr8_2:4666357-4666376 Msa1229050:intron 55.0%
! GGCCAATGAAGCAGTTCTGG+TGG - chr8_2:4666447-4666466 None:intergenic 55.0%
!! GCATCGGTGACTGGTTTGGA+GGG - chr8_2:4666493-4666512 None:intergenic 55.0%
! GGCCGGCTAAACTTTTGACG+TGG + chr8_2:4667283-4667302 Msa1229050:intron 55.0%
AGGACGAAGTTTACCGCGTG+TGG + chr8_2:4668263-4668282 Msa1229050:CDS 55.0%
GGGGGCAGAAAAGCTTTACG+AGG + chr8_2:4668372-4668391 Msa1229050:CDS 55.0%
!! TTCTGAGGCGCTTTCGTGCT+TGG + chr8_2:4668579-4668598 Msa1229050:CDS 55.0%
CCGCATACGCTTCGTCGTTA+TGG - chr8_2:4668744-4668763 None:intergenic 55.0%
GTCCTCGATGTGTGTGTGTG+TGG + chr8_2:4669042-4669061 Msa1229050:intron 55.0%
ACCCACACACACACACATCG+AGG - chr8_2:4669047-4669066 None:intergenic 55.0%
!! GGCATCGGTGACTGGTTTGG+AGG - chr8_2:4666494-4666513 None:intergenic 60.0%
!! GGAGGCATCGGTGACTGGTT+TGG - chr8_2:4666497-4666516 None:intergenic 60.0%
!! CTGATGGAGGCATCGGTGAC+TGG - chr8_2:4666502-4666521 None:intergenic 60.0%
!! AAACGACATGGAGAGCGCCG+AGG + chr8_2:4668687-4668706 Msa1229050:CDS 60.0%
Chromosome Type Strat End Strand Name
chr8_2 gene 4665533 4669328 4665533 ID=Msa1229050;Name=Msa1229050
chr8_2 mRNA 4665533 4669328 4665533 ID=Msa1229050-mRNA-1;Parent=Msa1229050;Name=Msa1229050-mRNA-1;_AED=0.15;_eAED=0.15;_QI=43|0.5|0.33|1|1|1|3|0|551
chr8_2 exon 4665533 4665971 4665533 ID=Msa1229050-mRNA-1:exon:1562;Parent=Msa1229050-mRNA-1
chr8_2 exon 4667647 4668820 4667647 ID=Msa1229050-mRNA-1:exon:1563;Parent=Msa1229050-mRNA-1
chr8_2 exon 4669243 4669328 4669243 ID=Msa1229050-mRNA-1:exon:1564;Parent=Msa1229050-mRNA-1
chr8_2 five_prime_UTR 4665533 4665575 4665533 ID=Msa1229050-mRNA-1:five_prime_utr;Parent=Msa1229050-mRNA-1
chr8_2 CDS 4665576 4665971 4665576 ID=Msa1229050-mRNA-1:cds;Parent=Msa1229050-mRNA-1
chr8_2 CDS 4667647 4668820 4667647 ID=Msa1229050-mRNA-1:cds;Parent=Msa1229050-mRNA-1
chr8_2 CDS 4669243 4669328 4669243 ID=Msa1229050-mRNA-1:cds;Parent=Msa1229050-mRNA-1
Gene Sequence

>Msa1229050

ATGATGACTACTATTACCCCTCTTCAAATTCAATCTTCTTTACAAAACCAAAACGTTTCTCTTTCCCTTTCTTATTCTCATACTCTTCCCCCCTTCAAACAAAGGGTCAATGTGTTTTACACTGACAGTGGAATTGGAATTGGAGGAGCAAGAAGAGTTTCTGTCACTTGTTCAATCTCAAAGATTCACAGTTATGGTACACTTGATTATGAGAGAAAACCCAATTTGAAATGGAATGCTATATACAAGAGGATTTCGATGATGGAGAATCCTGAATTGGGTTCTGCTTCTGTGTTGAATCAATGGGAGAATGAAGGAAGGAATCTCACCAAATGGGAACTTTGCAGAGTTGTTAAAGAGTTGAGAAAGTACAGAAAACATGATAGAGCTCTTCAGGTATATGATTGGATGAACAATAGACCAGAGAGGTTTAGAATATCTGCAAGTGACGTTGCGATTCAGTTAGATCTAATCGCAAGAGTTCACGGAGTTTCTAGTGCAGAAGGTTTTTTTCTGAACCTAACCAATGATTTAAAAGACAAGAGAACATATGGTGCCCTCTTGAATGCATATGTTCATTCTAGATCAAGAGAAAAGGCAGAATCTTTACTCGAAGTAATGAGGAGTAAAAGGTACTTAATACATTCACTACCGTTTAATTTAATGATGACTCTGTACATGAACCTTAAGGATTATGATAAAGTCGATATGTTGGTTTCAGAAATGAAAGAAAAGAACATACAGCTAGATATATATACATATAATATCTGGTTGTCCTCTTGTGGATCTCAAGGATCAATAGAGAAGATGGAACAAGTTTTTGAACAGATGTCAAAGGATCCTACCATTATTCCTAATTGGAGTACATTTAGTACAATGGCTGCAATGTATATTAAGATGGAACTGTTTGAAAAAGCACAAGAGTGCTTAAAAAAGGCCGAGGGTAGAATATTAGGTCGAGATAAAGTACCTTTTCATTATCTCCTGAGTTTGTATGGAAGTGTTGGAAACAAGGACGAAGTTTACCGCGTGTGGAATAACTATAAATCAATGTTTCCAAGTATACCTAACTTGGGATACCATGCTGTTATTTCTTCTCTCGTAAGAATGGACGATATTGAGGGGGCAGAAAAGCTTTACGAGGAATGGGTTTCTGTAAGGCCCTCTGATGATTCTAGAATAGGAAACCTTCTCATAAGCTGGTATCTTAAGAAAGGTAAATCAGATAAAGTTTTTAGCTTCTTTAAGCATATGAGTGAAGGTGGTGGATGTCCAAATTCGACTACATGGGAGCTTCTTTCTGAGGGTCATATTGCTGAAAATAGGGTTTCTGAGGCGCTTTCGTGCTTGGAAAAAGCTTTTACGACAAGTGATTCAAAGAGTTGGAAACCCAAGCCAATAAAATTGGCTGCATTCCTTAAGCTTTGTCAAGACGAAAACGACATGGAGAGCGCCGAGGTTTTAATTGAGTTGTTGAAGAAGTCGGGATACCATAACGACGAAGCGTATGCGGCACTCATTAGCAAAGATGAACCGTCAAACAGGATTGAAAGAATTGATGATGTTGACAGAATTGACTCTTCTGATGCATGTGATATGAGGCCATCCTTGTGTATGTTTTCTATGACTTGTGTCTTTAAATTTGGAATGAAGTAG
Protein sequence

>Msa1229050

MMTTITPLQIQSSLQNQNVSLSLSYSHTLPPFKQRVNVFYTDSGIGIGGARRVSVTCSISKIHSYGTLDYERKPNLKWNAIYKRISMMENPELGSASVLNQWENEGRNLTKWELCRVVKELRKYRKHDRALQVYDWMNNRPERFRISASDVAIQLDLIARVHGVSSAEGFFLNLTNDLKDKRTYGALLNAYVHSRSREKAESLLEVMRSKRYLIHSLPFNLMMTLYMNLKDYDKVDMLVSEMKEKNIQLDIYTYNIWLSSCGSQGSIEKMEQVFEQMSKDPTIIPNWSTFSTMAAMYIKMELFEKAQECLKKAEGRILGRDKVPFHYLLSLYGSVGNKDEVYRVWNNYKSMFPSIPNLGYHAVISSLVRMDDIEGAEKLYEEWVSVRPSDDSRIGNLLISWYLKKGKSDKVFSFFKHMSEGGGCPNSTTWELLSEGHIAENRVSEALSCLEKAFTTSDSKSWKPKPIKLAAFLKLCQDENDMESAEVLIELLKKSGYHNDEAYAALISKDEPSNRIERIDDVDRIDSSDACDMRPSLCMFSMTCVFKFGMK*