AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1419320


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1419320 MtrunA17_Chr1g0154571 85.047 214 3 1 1 214 77 261 4.51e-126 356
Msa1419320 MtrunA17_Chr3g0141331 70.755 212 60 1 1 212 83 292 9.70e-105 304
Msa1419320 MtrunA17_Chr5g0403531 60.870 207 80 1 1 207 80 285 1.11e-85 255
Msa1419320 MtrunA17_Chr8g0373641 61.881 202 74 2 1 200 85 285 1.51e-84 253
Msa1419320 MtrunA17_Chr7g0273031 35.057 174 105 3 39 204 101 274 2.11e-28 107
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1419320 AT4G36830.1 63.861 202 71 2 1 200 78 279 5.57e-91 269
Msa1419320 AT1G75000.1 65.854 205 67 2 1 202 76 280 3.42e-89 264
Msa1419320 AT4G36830.2 66.298 181 59 2 1 179 78 258 3.18e-84 252
Msa1419320 AT3G06460.1 33.140 172 104 4 39 205 105 270 1.45e-23 95.9
Msa1419320 AT3G06470.1 29.947 187 114 5 29 202 95 277 4.02e-23 94.4

Find 32 sgRNAs with CRISPR-Local

Find 56 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTAATTTGGTTTGTCATTT+TGG 0.099853 tig0024705:+3220 Msa1419320:CDS
TTGGTTTATTGTTTGGTTTA+TGG 0.193155 tig0024705:+3118 Msa1419320:CDS
TGGTTTATGGCTATAGATTT+TGG 0.209259 tig0024705:+3131 Msa1419320:CDS
CTCGACTTTGGTTTATTGTT+TGG 0.257456 tig0024705:+3111 Msa1419320:CDS
AGGTGCTATTGTTCTTCTTT+TGG 0.268883 tig0024705:-3363 None:intergenic
GGTGTAATGGAATTGGAGCT+TGG 0.382997 tig0024705:+3272 Msa1419320:CDS
AGAGGTGGGTGTAATGGAAT+TGG 0.394775 tig0024705:+3265 Msa1419320:CDS
GTGTAATGGAATTGGAGCTT+GGG 0.420847 tig0024705:+3273 Msa1419320:CDS
CCACTTGGTATGCGTCCATC+TGG 0.425938 tig0024705:+2902 Msa1419320:CDS
TTGATCAGATGTACTACTAT+AGG 0.432647 tig0024705:-3383 None:intergenic
AAACAAAGGAACCATTCAAA+AGG 0.452719 tig0024705:-2878 None:intergenic
AAAATGAGAGTTTCCGTTGA+CGG 0.478008 tig0024705:-3000 None:intergenic
AAAAGAACTGATCAAGAGAA+TGG 0.488684 tig0024705:-3041 None:intergenic
TGGTTCCTTTGTTTCCCACT+TGG 0.489824 tig0024705:+2887 Msa1419320:CDS
AACAAAGGAACCATTCAAAA+GGG 0.499822 tig0024705:-2877 None:intergenic
TTGGCAATGAACATTGACAT+AGG 0.540007 tig0024705:-3344 None:intergenic
TCTTTGTCATTCTCCGTCAA+CGG 0.544484 tig0024705:+2987 Msa1419320:CDS
ACGACACACGGTGGCTACTC+CGG 0.551699 tig0024705:+2840 Msa1419320:CDS
GGAGTAGCCACCGTGTGTCG+TGG 0.569741 tig0024705:-2838 None:intergenic
CAGAAGAAAACACGACCAGA+TGG 0.581311 tig0024705:-2917 None:intergenic
CTGCAGAACTCCACGACACA+CGG 0.598573 tig0024705:+2828 Msa1419320:CDS
GTCGTGGAGTTCTGCAGCCG+CGG 0.601574 tig0024705:-2822 None:intergenic
GCATACCAAGTGGGAAACAA+AGG 0.605927 tig0024705:-2892 None:intergenic
TTGACGGAGAATGACAAAGA+AGG 0.617040 tig0024705:-2984 None:intergenic
AGCCGCGGAGAATAGCATGC+CGG 0.623890 tig0024705:-2807 None:intergenic
CATGCCGGAGAAAATTGTGA+TGG 0.634231 tig0024705:-2792 None:intergenic
CCAGATGGACGCATACCAAG+TGG 0.648526 tig0024705:-2902 None:intergenic
ACAAAGGAACCATTCAAAAG+GGG 0.655501 tig0024705:-2876 None:intergenic
ACAAATGAAGAAACCTTGAG+AGG 0.668969 tig0024705:-2955 None:intergenic
CAGATGGACGCATACCAAGT+GGG 0.681279 tig0024705:-2901 None:intergenic
CTCCGGCATGCTATTCTCCG+CGG 0.683685 tig0024705:+2805 Msa1419320:CDS
CAGAACTCCACGACACACGG+TGG 0.748763 tig0024705:+2831 Msa1419320:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATAGATTTTGGATTGAAAT+TGG + tig0024705:3142-3161 Msa1419320:CDS 20.0%
!!! ATAGATTTTGGATTGAAATT+GGG + tig0024705:3143-3162 Msa1419320:CDS 20.0%
!! GTGAAATAAAAATTCTTACT+TGG - tig0024705:3172-3191 None:intergenic 20.0%
!!! AATTGTCAAATGATTTTGTT+GGG + tig0024705:3196-3215 Msa1419320:CDS 20.0%
!! TTGGTTTATTGTTTGGTTTA+TGG + tig0024705:3118-3137 Msa1419320:CDS 25.0%
!! GAATTGTCAAATGATTTTGT+TGG + tig0024705:3195-3214 Msa1419320:CDS 25.0%
!!! TGTAATTTGGTTTGTCATTT+TGG + tig0024705:3220-3239 Msa1419320:CDS 25.0%
AACAAAGGAACCATTCAAAA+GGG - tig0024705:2880-2899 None:intergenic 30.0%
AAACAAAGGAACCATTCAAA+AGG - tig0024705:2881-2900 None:intergenic 30.0%
! CTTATATTTTCTACCTCTCA+AGG + tig0024705:2942-2961 Msa1419320:CDS 30.0%
AAAAGAACTGATCAAGAGAA+TGG - tig0024705:3044-3063 None:intergenic 30.0%
! TCTTGATCAGTTCTTTTCTA+TGG + tig0024705:3047-3066 Msa1419320:CDS 30.0%
! TGAATTTTCACAGTCTTTTC+AGG + tig0024705:3072-3091 Msa1419320:CDS 30.0%
! TGGTTTATGGCTATAGATTT+TGG + tig0024705:3131-3150 Msa1419320:CDS 30.0%
!! GATTTTGTTGGGTTGTAATT+TGG + tig0024705:3207-3226 Msa1419320:CDS 30.0%
TTGATCAGATGTACTACTAT+AGG - tig0024705:3386-3405 None:intergenic 30.0%
! ATCTCCATCACAATTTTCTC+CGG + tig0024705:2788-2807 Msa1419320:CDS 35.0%
! CTAAAACTACCCCTTTTGAA+TGG + tig0024705:2867-2886 Msa1419320:CDS 35.0%
ACAAAGGAACCATTCAAAAG+GGG - tig0024705:2879-2898 None:intergenic 35.0%
ACAAATGAAGAAACCTTGAG+AGG - tig0024705:2958-2977 None:intergenic 35.0%
AAAATGAGAGTTTCCGTTGA+CGG - tig0024705:3003-3022 None:intergenic 35.0%
!! AACGGAAACTCTCATTTTTC+AGG + tig0024705:3005-3024 Msa1419320:CDS 35.0%
! CTCGACTTTGGTTTATTGTT+TGG + tig0024705:3111-3130 Msa1419320:CDS 35.0%
!!! TTTGTCATTTTGGTGTGCTT+TGG + tig0024705:3230-3249 Msa1419320:CDS 35.0%
TGACATAGGACTTCAAAAAG+AGG - tig0024705:3333-3352 None:intergenic 35.0%
TTGGCAATGAACATTGACAT+AGG - tig0024705:3347-3366 None:intergenic 35.0%
!!! AGGTGCTATTGTTCTTCTTT+TGG - tig0024705:3366-3385 None:intergenic 35.0%
!!! AAGGGGTAGTTTTAGTTCTC+CGG - tig0024705:2862-2881 None:intergenic 40.0%
TTGACGGAGAATGACAAAGA+AGG - tig0024705:2987-3006 None:intergenic 40.0%
TCTTTGTCATTCTCCGTCAA+CGG + tig0024705:2987-3006 Msa1419320:CDS 40.0%
!!! TCACAGTCTTTTCAGGTTCT+TGG + tig0024705:3079-3098 Msa1419320:CDS 40.0%
!!! CACAGTCTTTTCAGGTTCTT+GGG + tig0024705:3080-3099 Msa1419320:CDS 40.0%
! GTGTGCTTTGGTTGCATTTT+TGG + tig0024705:3242-3261 Msa1419320:CDS 40.0%
!! CTTTGGTTGCATTTTTGGAG+AGG + tig0024705:3247-3266 Msa1419320:CDS 40.0%
! GTGTAATGGAATTGGAGCTT+GGG + tig0024705:3273-3292 Msa1419320:CDS 40.0%
CATGCCGGAGAAAATTGTGA+TGG - tig0024705:2795-2814 None:intergenic 45.0%
! TGGTTCCTTTGTTTCCCACT+TGG + tig0024705:2887-2906 Msa1419320:CDS 45.0%
GCATACCAAGTGGGAAACAA+AGG - tig0024705:2895-2914 None:intergenic 45.0%
CAGAAGAAAACACGACCAGA+TGG - tig0024705:2920-2939 None:intergenic 45.0%
! CATCTGGTCGTGTTTTCTTC+TGG + tig0024705:2918-2937 Msa1419320:CDS 45.0%
! TGGGATTTTGCTCTCGACTT+TGG + tig0024705:3099-3118 Msa1419320:CDS 45.0%
!! TGGTTGCATTTTTGGAGAGG+TGG + tig0024705:3250-3269 Msa1419320:CDS 45.0%
!! GGTTGCATTTTTGGAGAGGT+GGG + tig0024705:3251-3270 Msa1419320:CDS 45.0%
!! TTTTGGAGAGGTGGGTGTAA+TGG + tig0024705:3259-3278 Msa1419320:CDS 45.0%
! AGAGGTGGGTGTAATGGAAT+TGG + tig0024705:3265-3284 Msa1419320:CDS 45.0%
! GGTGTAATGGAATTGGAGCT+TGG + tig0024705:3272-3291 Msa1419320:CDS 45.0%
CAGATGGACGCATACCAAGT+GGG - tig0024705:2904-2923 None:intergenic 50.0%
CTGCAGAACTCCACGACACA+CGG + tig0024705:2828-2847 Msa1419320:CDS 55.0%
CCAGATGGACGCATACCAAG+TGG - tig0024705:2905-2924 None:intergenic 55.0%
CCACTTGGTATGCGTCCATC+TGG + tig0024705:2902-2921 Msa1419320:CDS 55.0%
CTCCGGCATGCTATTCTCCG+CGG + tig0024705:2805-2824 Msa1419320:CDS 60.0%
AGCCGCGGAGAATAGCATGC+CGG - tig0024705:2810-2829 None:intergenic 60.0%
CAGAACTCCACGACACACGG+TGG + tig0024705:2831-2850 Msa1419320:CDS 60.0%
ACGACACACGGTGGCTACTC+CGG + tig0024705:2840-2859 Msa1419320:CDS 60.0%
GTCGTGGAGTTCTGCAGCCG+CGG - tig0024705:2825-2844 None:intergenic 65.0%
GGAGTAGCCACCGTGTGTCG+TGG - tig0024705:2841-2860 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
tig0024705 gene 2779 3423 2779 ID=Msa1419320;Name=Msa1419320
tig0024705 mRNA 2779 3423 2779 ID=Msa1419320-mRNA-1;Parent=Msa1419320;Name=Msa1419320-mRNA-1;_AED=0.18;_eAED=0.18;_QI=0|-1|0|1|-1|1|1|0|214
tig0024705 exon 2779 3423 2779 ID=Msa1419320-mRNA-1:exon:833;Parent=Msa1419320-mRNA-1
tig0024705 CDS 2779 3423 2779 ID=Msa1419320-mRNA-1:cds;Parent=Msa1419320-mRNA-1
Gene Sequence

>Msa1419320

ATGTCACTAATCTCCATCACAATTTTCTCCGGCATGCTATTCTCCGCGGCTGCAGAACTCCACGACACACGGTGGCTACTCCGGAGAACTAAAACTACCCCTTTTGAATGGTTCCTTTGTTTCCCACTTGGTATGCGTCCATCTGGTCGTGTTTTCTTCTGGTCTTATATTTTCTACCTCTCAAGGTTTCTTCATTTGTTTCGAACCTTCTTTGTCATTCTCCGTCAACGGAAACTCTCATTTTTCAGGCTATTCAACAACTCCATTCTCTTGATCAGTTCTTTTCTATGGCTTGAATTTTCACAGTCTTTTCAGGTTCTTGGGATTTTGCTCTCGACTTTGGTTTATTGTTTGGTTTATGGCTATAGATTTTGGATTGAAATTGGGTTGCCAAGTAAGAATTTTTATTTCACAGTGAATTGTCAAATGATTTTGTTGGGTTGTAATTTGGTTTGTCATTTTGGTGTGCTTTGGTTGCATTTTTGGAGAGGTGGGTGTAATGGAATTGGAGCTTGGGTTTTCAATTCTGTGTTGAATGCTGCTATTCTTTTCCTCTTTTTGAAGTCCTATGTCAATGTTCATTGCCAAAAGAAGAACAATAGCACCTATAGTAGTACATCTGATCAAAGTTTTAAATTGCAGTAA

Protein sequence

>Msa1419320

MSLISITIFSGMLFSAAAELHDTRWLLRRTKTTPFEWFLCFPLGMRPSGRVFFWSYIFYLSRFLHLFRTFFVILRQRKLSFFRLFNNSILLISSFLWLEFSQSFQVLGILLSTLVYCLVYGYRFWIEIGLPSKNFYFTVNCQMILLGCNLVCHFGVLWLHFWRGGCNGIGAWVFNSVLNAAILFLFLKSYVNVHCQKKNNSTYSSTSDQSFKLQ*