AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1422890


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1422890 MtrunA17_Chr8g0368511 83.871 124 8 2 1 122 1 114 6.24e-70 206
Msa1422890 MtrunA17_Chr7g0263161 48.529 68 35 0 64 131 15 82 6.05e-19 75.9
Msa1422890 MtrunA17_Chr7g0222601 49.254 67 24 1 47 113 15 71 7.05e-19 76.3
Msa1422890 MtrunA17_Chr1g0212831 46.970 66 25 1 46 111 9 64 2.66e-16 69.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1422890 AT1G10657.2 48.454 97 39 2 25 121 1 86 4.41e-27 97.4
Msa1422890 AT1G10657.3 48.454 97 39 2 25 121 1 86 4.78e-27 97.4
Msa1422890 AT1G10657.1 38.843 121 39 3 25 121 1 110 1.64e-21 84.0
Msa1422890 AT1G10657.4 38.843 121 39 3 25 121 1 110 1.84e-21 83.6
Msa1422890 AT3G55240.1 51.515 66 22 1 46 111 8 63 5.88e-20 79.0
Msa1422890 AT3G28990.1 37.805 82 41 1 41 122 3 74 4.29e-16 68.9
Msa1422890 AT5G02580.1 49.180 61 29 1 64 122 18 78 1.97e-15 67.4
Msa1422890 AT5G02580.3 49.180 61 29 1 64 122 11 71 3.82e-15 66.2
Msa1422890 AT2G46420.2 53.061 49 23 0 63 111 57 105 4.25e-11 59.7
Msa1422890 AT2G46420.3 53.061 49 23 0 63 111 57 105 4.75e-11 59.7
Msa1422890 AT2G46420.1 53.061 49 23 0 63 111 57 105 4.75e-11 59.7

Find 35 sgRNAs with CRISPR-Local

Find 119 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGGATTTCATGAGCTATTT+TGG 0.125606 tig0025492:-17552 Msa1422890:five_prime_UTR
CAGCCACTTGTTACTCTTAC+AGG 0.198423 tig0025492:-17061 Msa1422890:intron
TATCATGGTGATTGATTAGA+TGG 0.281103 tig0025492:-16439 Msa1422890:three_prime_UTR
GTGCTTAATTCTGTTGCTAA+TGG 0.321815 tig0025492:+17381 None:intergenic
ATGGTATTGGTTATAACTTT+TGG 0.325626 tig0025492:-16263 Msa1422890:three_prime_UTR
CTTCTAGTGCCTTTATGCAT+TGG 0.385653 tig0025492:+17098 None:intergenic
AAAGTCAAAGTTGTACGATA+TGG 0.388439 tig0025492:-16287 Msa1422890:three_prime_UTR
GATGAGGTAAATCTATCCTT+TGG 0.389875 tig0025492:-17571 None:intergenic
ATTGTAAATGAAGCTGTCAT+TGG 0.397705 tig0025492:-16121 Msa1422890:three_prime_UTR
GAAGTTGTATATGATTTGTT+TGG 0.409684 tig0025492:-16414 Msa1422890:three_prime_UTR
AGATCATGCATCTCTCTATC+AGG 0.411660 tig0025492:+17137 None:intergenic
TGTACGATATGGCATGGTAT+TGG 0.426671 tig0025492:-16276 Msa1422890:three_prime_UTR
TTTACGTACGTGAACGAGCT+TGG 0.432955 tig0025492:+16877 None:intergenic
ATTATGGTCACAAAGTCTAA+TGG 0.452405 tig0025492:+16382 None:intergenic
ACAAACAAGGAAGAGGGTAT+TGG 0.476829 tig0025492:+17340 None:intergenic
GAGTCAAGACCAATGCATAA+AGG 0.483045 tig0025492:-17107 Msa1422890:CDS
TGTTGTGTTAAATATTATCA+TGG 0.487165 tig0025492:-16454 Msa1422890:three_prime_UTR
CGTACGTGAACGAGCTTGGA+GGG 0.493662 tig0025492:+16881 None:intergenic
TCCACATAGCTATATTAGAA+TGG 0.532785 tig0025492:-17310 Msa1422890:intron
ACGTACGTGAACGAGCTTGG+AGG 0.547000 tig0025492:+16880 None:intergenic
GTCGATTGAGGACTTACACT+TGG 0.554388 tig0025492:+16240 None:intergenic
TGACAATACAAACAAGGAAG+AGG 0.554518 tig0025492:+17333 None:intergenic
TATATCATTATCTAGAGTGA+AGG 0.560952 tig0025492:+17528 None:intergenic
AAGGCACTAGAAGAACATGC+AGG 0.583674 tig0025492:-17088 Msa1422890:CDS
AAATGATCAACAAAGTGAGA+TGG 0.584027 tig0025492:-16489 Msa1422890:CDS
ATATCATTATCTAGAGTGAA+GGG 0.597837 tig0025492:+17529 None:intergenic
AGTGTGGAGAGAGTTGCAAA+AGG 0.597912 tig0025492:-16944 Msa1422890:CDS
CAAAGTTGTACGATATGGCA+TGG 0.604780 tig0025492:-16282 Msa1422890:three_prime_UTR
GACAATACAAACAAGGAAGA+GGG 0.614485 tig0025492:+17334 None:intergenic
AAATAGCTCATGAAATCCAA+AGG 0.624632 tig0025492:+17555 None:intergenic
AGGCACTAGAAGAACATGCA+GGG 0.629832 tig0025492:-17087 Msa1422890:CDS
GATAATGATATATATATGTG+TGG 0.633681 tig0025492:-17517 Msa1422890:five_prime_UTR
ACCATTCTAATATAGCTATG+TGG 0.637830 tig0025492:+17309 None:intergenic
AGACCTGTAAGAGTAACAAG+TGG 0.663372 tig0025492:+17058 None:intergenic
TGTGGATGACAATACAAACA+AGG 0.679966 tig0025492:+17327 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAATTATATACATAGATTAA+TGG + tig0025492:16919-16938 None:intergenic 10.0%
!! ATATATATATATATGCATTA+TGG + tig0025492:17303-17322 None:intergenic 10.0%
!! AATAATCATTCTTATAAATT+TGG - tig0025492:17568-17587 Msa1422890:five_prime_UTR 10.0%
!! TAATTATATACATAGATTAA+TGG + tig0025492:16919-16938 None:intergenic 10.0%
!! ATATATATATATATGCATTA+TGG + tig0025492:17303-17322 None:intergenic 10.0%
!! TTATTATGATATTGAAAACA+AGG + tig0025492:16470-16489 None:intergenic 15.0%
!!! AAATTCCATATATATTTTCA+AGG - tig0025492:16818-16837 Msa1422890:intron 15.0%
!!! GGTATTTTTTAAAAATCAAT+AGG + tig0025492:16966-16985 None:intergenic 15.0%
!!! TCTTATTTTATTTACTTTTC+AGG - tig0025492:17129-17148 Msa1422890:CDS 15.0%
!!! TAGTTTTTAAATATTTGATG+TGG - tig0025492:17345-17364 Msa1422890:CDS 15.0%
!!! AATTTTTAAAGTTAAAGTTG+AGG - tig0025492:17486-17505 Msa1422890:five_prime_UTR 15.0%
!! TTATTATGATATTGAAAACA+AGG + tig0025492:16470-16489 None:intergenic 15.0%
!!! AAATTCCATATATATTTTCA+AGG - tig0025492:16818-16837 Msa1422890:intron 15.0%
!!! GGTATTTTTTAAAAATCAAT+AGG + tig0025492:16966-16985 None:intergenic 15.0%
!!! TCTTATTTTATTTACTTTTC+AGG - tig0025492:17129-17148 Msa1422890:CDS 15.0%
!!! TAGTTTTTAAATATTTGATG+TGG - tig0025492:17345-17364 Msa1422890:CDS 15.0%
!!! AATTTTTAAAGTTAAAGTTG+AGG - tig0025492:17486-17505 Msa1422890:five_prime_UTR 15.0%
!! GATAATGATATATATATGTG+TGG - tig0025492:16149-16168 Msa1422890:three_prime_UTR 20.0%
!! ACTAAATTATAAGGAAGAAA+AGG - tig0025492:16883-16902 Msa1422890:CDS 20.0%
!!! TATGTATGATATTTTGCTTT+TGG + tig0025492:16995-17014 None:intergenic 20.0%
!! TGTTGTGTTAAATATTATCA+TGG - tig0025492:17212-17231 Msa1422890:intron 20.0%
!! GATAATGATATATATATGTG+TGG - tig0025492:16149-16168 Msa1422890:three_prime_UTR 20.0%
!! ACTAAATTATAAGGAAGAAA+AGG - tig0025492:16883-16902 Msa1422890:CDS 20.0%
!!! TATGTATGATATTTTGCTTT+TGG + tig0025492:16995-17014 None:intergenic 20.0%
!! TGTTGTGTTAAATATTATCA+TGG - tig0025492:17212-17231 Msa1422890:intron 20.0%
!! ATATCATTATCTAGAGTGAA+GGG + tig0025492:16140-16159 None:intergenic 25.0%
!! TATATCATTATCTAGAGTGA+AGG + tig0025492:16141-16160 None:intergenic 25.0%
!! TTTTCCATGCATGAAAATTT+TGG + tig0025492:16496-16515 None:intergenic 25.0%
! TTGAAACTTATGTTACACTT+TGG - tig0025492:16656-16675 Msa1422890:intron 25.0%
! TGAAACTTATGTTACACTTT+GGG - tig0025492:16657-16676 Msa1422890:intron 25.0%
! CTGATCCTTGAAAATATATA+TGG + tig0025492:16826-16845 None:intergenic 25.0%
! TATGCTTTGCATATCATTAA+AGG + tig0025492:16852-16871 None:intergenic 25.0%
! ATGCATATAGCATTAGTTAA+TGG + tig0025492:16943-16962 None:intergenic 25.0%
!!! GTCATTTTGAAAAGAAACAT+TGG - tig0025492:17154-17173 Msa1422890:CDS 25.0%
!!! GAAGTTGTATATGATTTGTT+TGG - tig0025492:17252-17271 Msa1422890:intron 25.0%
!!! ATGGTATTGGTTATAACTTT+TGG - tig0025492:17403-17422 Msa1422890:CDS 25.0%
!! ATATCATTATCTAGAGTGAA+GGG + tig0025492:16140-16159 None:intergenic 25.0%
!! TATATCATTATCTAGAGTGA+AGG + tig0025492:16141-16160 None:intergenic 25.0%
!! TTTTCCATGCATGAAAATTT+TGG + tig0025492:16496-16515 None:intergenic 25.0%
! TTGAAACTTATGTTACACTT+TGG - tig0025492:16656-16675 Msa1422890:intron 25.0%
! TGAAACTTATGTTACACTTT+GGG - tig0025492:16657-16676 Msa1422890:intron 25.0%
! CTGATCCTTGAAAATATATA+TGG + tig0025492:16826-16845 None:intergenic 25.0%
! TATGCTTTGCATATCATTAA+AGG + tig0025492:16852-16871 None:intergenic 25.0%
! ATGCATATAGCATTAGTTAA+TGG + tig0025492:16943-16962 None:intergenic 25.0%
!!! GTCATTTTGAAAAGAAACAT+TGG - tig0025492:17154-17173 Msa1422890:CDS 25.0%
!!! GAAGTTGTATATGATTTGTT+TGG - tig0025492:17252-17271 Msa1422890:intron 25.0%
!!! ATGGTATTGGTTATAACTTT+TGG - tig0025492:17403-17422 Msa1422890:CDS 25.0%
AAATAGCTCATGAAATCCAA+AGG + tig0025492:16114-16133 None:intergenic 30.0%
! TTGGATTTCATGAGCTATTT+TGG - tig0025492:16114-16133 Msa1422890:three_prime_UTR 30.0%
TCCACATAGCTATATTAGAA+TGG - tig0025492:16356-16375 Msa1422890:three_prime_UTR 30.0%
ACCATTCTAATATAGCTATG+TGG + tig0025492:16360-16379 None:intergenic 30.0%
TTTCATGCATGGAAAAATGT+AGG - tig0025492:16500-16519 Msa1422890:CDS 30.0%
! TACACTTTGGGAAACATATA+TGG - tig0025492:16669-16688 Msa1422890:intron 30.0%
! TGTATATTCATTTTGCAGTG+TGG - tig0025492:16706-16725 Msa1422890:intron 30.0%
!! ATATTTTGCTTTTGGACAGT+TGG + tig0025492:16987-17006 None:intergenic 30.0%
AAATGATCAACAAAGTGAGA+TGG - tig0025492:17177-17196 Msa1422890:CDS 30.0%
TATCATGGTGATTGATTAGA+TGG - tig0025492:17227-17246 Msa1422890:intron 30.0%
ATTATGGTCACAAAGTCTAA+TGG + tig0025492:17287-17306 None:intergenic 30.0%
AAAGTCAAAGTTGTACGATA+TGG - tig0025492:17379-17398 Msa1422890:CDS 30.0%
ATTGTAAATGAAGCTGTCAT+TGG - tig0025492:17545-17564 Msa1422890:five_prime_UTR 30.0%
AAATAGCTCATGAAATCCAA+AGG + tig0025492:16114-16133 None:intergenic 30.0%
! TTGGATTTCATGAGCTATTT+TGG - tig0025492:16114-16133 Msa1422890:three_prime_UTR 30.0%
TCCACATAGCTATATTAGAA+TGG - tig0025492:16356-16375 Msa1422890:three_prime_UTR 30.0%
ACCATTCTAATATAGCTATG+TGG + tig0025492:16360-16379 None:intergenic 30.0%
TTTCATGCATGGAAAAATGT+AGG - tig0025492:16500-16519 Msa1422890:CDS 30.0%
! TACACTTTGGGAAACATATA+TGG - tig0025492:16669-16688 Msa1422890:intron 30.0%
! TGTATATTCATTTTGCAGTG+TGG - tig0025492:16706-16725 Msa1422890:intron 30.0%
!! ATATTTTGCTTTTGGACAGT+TGG + tig0025492:16987-17006 None:intergenic 30.0%
AAATGATCAACAAAGTGAGA+TGG - tig0025492:17177-17196 Msa1422890:CDS 30.0%
TATCATGGTGATTGATTAGA+TGG - tig0025492:17227-17246 Msa1422890:intron 30.0%
ATTATGGTCACAAAGTCTAA+TGG + tig0025492:17287-17306 None:intergenic 30.0%
AAAGTCAAAGTTGTACGATA+TGG - tig0025492:17379-17398 Msa1422890:CDS 30.0%
ATTGTAAATGAAGCTGTCAT+TGG - tig0025492:17545-17564 Msa1422890:five_prime_UTR 30.0%
GTGCTTAATTCTGTTGCTAA+TGG + tig0025492:16288-16307 None:intergenic 35.0%
GACAATACAAACAAGGAAGA+GGG + tig0025492:16335-16354 None:intergenic 35.0%
TGACAATACAAACAAGGAAG+AGG + tig0025492:16336-16355 None:intergenic 35.0%
TGTGGATGACAATACAAACA+AGG + tig0025492:16342-16361 None:intergenic 35.0%
! AGCTCCAAAATTTTCATGCA+TGG - tig0025492:16489-16508 Msa1422890:CDS 35.0%
!! TGAATCACTTTTGTCGATTG+AGG + tig0025492:17441-17460 None:intergenic 35.0%
GTGCTTAATTCTGTTGCTAA+TGG + tig0025492:16288-16307 None:intergenic 35.0%
GACAATACAAACAAGGAAGA+GGG + tig0025492:16335-16354 None:intergenic 35.0%
TGACAATACAAACAAGGAAG+AGG + tig0025492:16336-16355 None:intergenic 35.0%
TGTGGATGACAATACAAACA+AGG + tig0025492:16342-16361 None:intergenic 35.0%
! AGCTCCAAAATTTTCATGCA+TGG - tig0025492:16489-16508 Msa1422890:CDS 35.0%
!! TGAATCACTTTTGTCGATTG+AGG + tig0025492:17441-17460 None:intergenic 35.0%
! ACAAACAAGGAAGAGGGTAT+TGG + tig0025492:16329-16348 None:intergenic 40.0%
AGATCATGCATCTCTCTATC+AGG + tig0025492:16532-16551 None:intergenic 40.0%
GAGTCAAGACCAATGCATAA+AGG - tig0025492:16559-16578 Msa1422890:intron 40.0%
CTTCTAGTGCCTTTATGCAT+TGG + tig0025492:16571-16590 None:intergenic 40.0%
AGACCTGTAAGAGTAACAAG+TGG + tig0025492:16611-16630 None:intergenic 40.0%
CAAAGTTGTACGATATGGCA+TGG - tig0025492:17384-17403 Msa1422890:CDS 40.0%
TGTACGATATGGCATGGTAT+TGG - tig0025492:17390-17409 Msa1422890:CDS 40.0%
! ACAAACAAGGAAGAGGGTAT+TGG + tig0025492:16329-16348 None:intergenic 40.0%
AGATCATGCATCTCTCTATC+AGG + tig0025492:16532-16551 None:intergenic 40.0%
GAGTCAAGACCAATGCATAA+AGG - tig0025492:16559-16578 Msa1422890:intron 40.0%
CTTCTAGTGCCTTTATGCAT+TGG + tig0025492:16571-16590 None:intergenic 40.0%
AGACCTGTAAGAGTAACAAG+TGG + tig0025492:16611-16630 None:intergenic 40.0%
CAAAGTTGTACGATATGGCA+TGG - tig0025492:17384-17403 Msa1422890:CDS 40.0%
TGTACGATATGGCATGGTAT+TGG - tig0025492:17390-17409 Msa1422890:CDS 40.0%
! AAGGCACTAGAAGAACATGC+AGG - tig0025492:16578-16597 Msa1422890:intron 45.0%
AGGCACTAGAAGAACATGCA+GGG - tig0025492:16579-16598 Msa1422890:intron 45.0%
! CAGCCACTTGTTACTCTTAC+AGG - tig0025492:16605-16624 Msa1422890:intron 45.0%
AGTGTGGAGAGAGTTGCAAA+AGG - tig0025492:16722-16741 Msa1422890:intron 45.0%
TTTACGTACGTGAACGAGCT+TGG + tig0025492:16792-16811 None:intergenic 45.0%
GTCGATTGAGGACTTACACT+TGG + tig0025492:17429-17448 None:intergenic 45.0%
! AAGGCACTAGAAGAACATGC+AGG - tig0025492:16578-16597 Msa1422890:intron 45.0%
AGGCACTAGAAGAACATGCA+GGG - tig0025492:16579-16598 Msa1422890:intron 45.0%
! CAGCCACTTGTTACTCTTAC+AGG - tig0025492:16605-16624 Msa1422890:intron 45.0%
AGTGTGGAGAGAGTTGCAAA+AGG - tig0025492:16722-16741 Msa1422890:intron 45.0%
TTTACGTACGTGAACGAGCT+TGG + tig0025492:16792-16811 None:intergenic 45.0%
GTCGATTGAGGACTTACACT+TGG + tig0025492:17429-17448 None:intergenic 45.0%
!!! TAAAGAATATTATTATTTTA+TGG - tig0025492:16435-16454 Msa1422890:three_prime_UTR 5.0%
!! ATAAATTTAACTAAATTATA+AGG - tig0025492:16874-16893 Msa1422890:intron 5.0%
!!! TAAAGAATATTATTATTTTA+TGG - tig0025492:16435-16454 Msa1422890:three_prime_UTR 5.0%
!! ATAAATTTAACTAAATTATA+AGG - tig0025492:16874-16893 Msa1422890:intron 5.0%
CGTACGTGAACGAGCTTGGA+GGG + tig0025492:16788-16807 None:intergenic 55.0%
ACGTACGTGAACGAGCTTGG+AGG + tig0025492:16789-16808 None:intergenic 55.0%
CGTACGTGAACGAGCTTGGA+GGG + tig0025492:16788-16807 None:intergenic 55.0%
ACGTACGTGAACGAGCTTGG+AGG + tig0025492:16789-16808 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
tig0025492 gene 16100 17588 16100 ID=Msa1422890;Name=Msa1422890
tig0025492 mRNA 16100 17588 16100 ID=Msa1422890-mRNA-1;Parent=Msa1422890;Name=Msa1422890-mRNA-1;_AED=0.23;_eAED=0.23;_QI=128|0.33|0.5|1|0.33|0.5|4|384|140
tig0025492 exon 16100 16537 16100 ID=Msa1422890-mRNA-1:exon:2952;Parent=Msa1422890-mRNA-1
tig0025492 exon 16882 16964 16882 ID=Msa1422890-mRNA-1:exon:2951;Parent=Msa1422890-mRNA-1
tig0025492 exon 17062 17197 17062 ID=Msa1422890-mRNA-1:exon:2950;Parent=Msa1422890-mRNA-1
tig0025492 exon 17311 17588 17311 ID=Msa1422890-mRNA-1:exon:2949;Parent=Msa1422890-mRNA-1
tig0025492 five_prime_UTR 17461 17588 17461 ID=Msa1422890-mRNA-1:five_prime_utr;Parent=Msa1422890-mRNA-1
tig0025492 CDS 17311 17460 17311 ID=Msa1422890-mRNA-1:cds;Parent=Msa1422890-mRNA-1
tig0025492 CDS 17062 17197 17062 ID=Msa1422890-mRNA-1:cds;Parent=Msa1422890-mRNA-1
tig0025492 CDS 16882 16964 16882 ID=Msa1422890-mRNA-1:cds;Parent=Msa1422890-mRNA-1
tig0025492 CDS 16484 16537 16484 ID=Msa1422890-mRNA-1:cds;Parent=Msa1422890-mRNA-1
tig0025492 three_prime_UTR 16100 16483 16100 ID=Msa1422890-mRNA-1:three_prime_utr;Parent=Msa1422890-mRNA-1
Gene Sequence

>Msa1422890

ATGTACTTTTTCTCTGTGTGCTTTCTTCTATTTCTTTCTATATTCTATTTGTTTTGTCCATTAGCAACAGAATTAAGCACAATGCATGATCACACTCACCAATACCCTCTTCCTTGTTTGTATTGTCATCCACATAGCTATATTAGAATGCTCCAAAATTTTCATGCATGGAAAAATGTAGGTTCAAACCTGATAGAGAGATGCATGATCTTTCACATGAGTCAAGACCAATGCATAAAGGCACTAGAAGAACATGCAGGGATTCAGCCACTTGTTACTCTTACAGTGTGGAGAGAGTTGCAAAAGGAGAATGAAGAGTTCTTTAGAGCATATTTGCAACAATTTATCCCTCCAAGCTCGTTCACGTACGTAGTCATTTTGAAAAGAAACATTGGAAATGATCAACAAAGTGAGATGGATTAA

Protein sequence

>Msa1422890

MYFFSVCFLLFLSIFYLFCPLATELSTMHDHTHQYPLPCLYCHPHSYIRMLQNFHAWKNVGSNLIERCMIFHMSQDQCIKALEEHAGIQPLVTLTVWRELQKENEEFFRAYLQQFIPPSSFTYVVILKRNIGNDQQSEMD*