AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene98461


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene98461.t1 MTR_5g028350 97.232 289 8 0 1 289 1 289 0.0 566
MS.gene98461.t1 MTR_8g075400 76.552 290 37 4 1 289 14 273 3.53e-138 391
MS.gene98461.t1 MTR_8g075400 87.500 224 26 1 1 224 1 222 7.17e-129 367
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene98461.t1 AT3G22660 65.188 293 87 6 2 290 12 293 1.91e-126 362

Find 70 sgRNAs with CRISPR-Local

Find 87 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCGGCCTCCAAAACCAAATT+TGG 0.189093 5.4:+18906516 None:intergenic
GATACTAAGAATGCCAAATT+TGG 0.209577 5.4:-18906529 MS.gene98461:CDS
GCTGAAACAACTAATGATTT+TGG 0.209631 5.4:-18906469 MS.gene98461:CDS
AAGAATGCCAAATTTGGTTT+TGG 0.274042 5.4:-18906523 MS.gene98461:CDS
GATTGAAATGGAGGTGTCTT+TGG 0.301021 5.4:-18907277 None:intergenic
TCAAAGCTCAAAGACTTATC+TGG 0.326167 5.4:+18906670 None:intergenic
AGAAAGGGCCAAGCAGAAAA+AGG 0.351345 5.4:-18906764 MS.gene98461:CDS
TCAGTTTCACCGTCAATCAT+TGG 0.370634 5.4:+18907243 None:intergenic
TCTGCTTCTTCGATCCCTTC+CGG 0.371874 5.4:+18906497 None:intergenic
TGCTTCCAATCCACATTCTC+AGG 0.372391 5.4:+18907093 None:intergenic
TGACGGTGAAACTGAAGATT+TGG 0.372926 5.4:-18907235 MS.gene98461:CDS
TTCAGTAAGGGTTCTGCTGC+TGG 0.375909 5.4:-18906442 MS.gene98461:CDS
TTACCTCCTGACCGATCTCC+TGG 0.396701 5.4:+18906598 None:intergenic
AGACAATCTCTTTGCTTCCC+TGG 0.401049 5.4:+18906813 None:intergenic
AGATAAGTCTTTGAGCTTTG+AGG 0.404345 5.4:-18906668 MS.gene98461:CDS
CTGAAGAGAGAAGAAAGGCC+AGG 0.422247 5.4:-18906831 MS.gene98461:CDS
CTCTCCATTGATATTGATCA+AGG 0.429280 5.4:-18907066 MS.gene98461:CDS
AGTCACAGAAATTGAAAGAA+AGG 0.458750 5.4:-18906780 MS.gene98461:CDS
TAGTCAGCAGGTCTCAAGAA+AGG 0.478435 5.4:+18906937 None:intergenic
CTTTGAGAAGCTTGAGTCGA+TGG 0.481104 5.4:-18906965 MS.gene98461:CDS
TGCTGACTATTACGCAGAAA+TGG 0.488469 5.4:-18906923 MS.gene98461:CDS
CTCCCCTTGATCAATATCAA+TGG 0.492911 5.4:+18907062 None:intergenic
CCGATCTCCTGGAGATACTC+CGG 0.494759 5.4:+18906609 None:intergenic
AGGAGATCGGTCAGGAGGTA+AGG 0.497659 5.4:-18906596 MS.gene98461:CDS
AGTCAGCAGGTCTCAAGAAA+GGG 0.501463 5.4:+18906938 None:intergenic
GGGAGATAGCCACATGGAAA+AGG 0.502899 5.4:-18906896 MS.gene98461:CDS
CATAAGCTGGCCTGAGAATG+TGG 0.503617 5.4:-18907103 MS.gene98461:CDS
TCTTCTGAATCTGATTCAAA+CGG 0.503965 5.4:+18907198 None:intergenic
GTCACAGAAATTGAAAGAAA+GGG 0.504083 5.4:-18906779 MS.gene98461:CDS
GCAAAGCAAGCTATGGGAAA+GGG 0.507585 5.4:-18906574 MS.gene98461:CDS
ATTAGATGAGAAGCGGAAGA+TGG 0.510158 5.4:-18906860 MS.gene98461:CDS
GGCATTGGAGGGAACTAGAC+AGG 0.514311 5.4:-18906989 MS.gene98461:CDS
AGGTAAGGCAAAGCAAGCTA+TGG 0.517763 5.4:-18906581 MS.gene98461:CDS
ATGGAGGTGTCTTTGGTTAA+CGG 0.521891 5.4:-18907270 MS.gene98461:CDS
TTTGAGAAGCTTGAGTCGAT+GGG 0.525398 5.4:-18906964 MS.gene98461:CDS
GGAGGCTGAAGAGAGAAGAA+AGG 0.530598 5.4:-18906836 MS.gene98461:CDS
CAAAGACTTATCTGGAGCAT+CGG 0.536868 5.4:+18906678 None:intergenic
AGATGAGAAGCGGAAGATGG+AGG 0.539024 5.4:-18906857 MS.gene98461:CDS
AACGGTGATCCAATGATTGA+CGG 0.554237 5.4:-18907252 MS.gene98461:CDS
AGCAAAGAGATTGTCTAAAG+AGG 0.557828 5.4:-18906806 MS.gene98461:CDS
AAGAGGCCCGGAGTATCTCC+AGG 0.558097 5.4:-18906616 MS.gene98461:CDS
CTCCATTGATATTGATCAAG+GGG 0.559298 5.4:-18907064 MS.gene98461:CDS
GTATCTCCAGGAGATCGGTC+AGG 0.562327 5.4:-18906604 MS.gene98461:CDS
CGATCTCCTGGAGATACTCC+GGG 0.563080 5.4:+18906610 None:intergenic
AGTAGATGTGAACGATGACT+TGG 0.565329 5.4:-18907037 MS.gene98461:CDS
ATTTCTGCGTAATAGTCAGC+AGG 0.568461 5.4:+18906925 None:intergenic
ATAAACTAGGAGACATAAGC+TGG 0.571619 5.4:-18907116 MS.gene98461:CDS
TGAAACTGAAGATTTGGATG+AGG 0.572001 5.4:-18907229 MS.gene98461:CDS
CCGGAGTATCTCCAGGAGAT+CGG 0.576336 5.4:-18906609 MS.gene98461:CDS
AAGTCTTTGAGCTTTGAGGA+TGG 0.576787 5.4:-18906664 MS.gene98461:CDS
TGAAGAGAGAAGAAAGGCCA+GGG 0.579670 5.4:-18906830 MS.gene98461:CDS
CATCGACTCAAGCTTCTCAA+AGG 0.582387 5.4:+18906966 None:intergenic
GGCAAAGCAAGCTATGGGAA+AGG 0.584114 5.4:-18906575 MS.gene98461:CDS
TCTCCATTGATATTGATCAA+GGG 0.594778 5.4:-18907065 MS.gene98461:CDS
GGTAAGGCAAAGCAAGCTAT+GGG 0.598074 5.4:-18906580 MS.gene98461:CDS
GGAAGCAGAGACAACAGAGT+GGG 0.599709 5.4:-18906714 MS.gene98461:CDS
ACATTGAATCTGTTAAGAAG+TGG 0.600860 5.4:-18906738 MS.gene98461:CDS
TGAGAAGCGGAAGATGGAGG+AGG 0.603783 5.4:-18906854 MS.gene98461:CDS
TTGAATCTGTTAAGAAGTGG+AGG 0.608378 5.4:-18906735 MS.gene98461:CDS
CTATTACGCAGAAATGGTGA+AGG 0.608653 5.4:-18906917 MS.gene98461:CDS
GTCGGCTATTAGATGAGAAG+CGG 0.609331 5.4:-18906867 MS.gene98461:CDS
GCTGGCCTGAGAATGTGGAT+TGG 0.611875 5.4:-18907098 MS.gene98461:CDS
ACATGGAAAAGGTGAAAAGT+CGG 0.619320 5.4:-18906885 MS.gene98461:CDS
CAACAGAGTGGGTTTGCTGA+CGG 0.625190 5.4:-18906703 MS.gene98461:CDS
AGGAAGCAGAGACAACAGAG+TGG 0.651328 5.4:-18906715 MS.gene98461:CDS
GATGCTATATTAGATAAACT+AGG 0.652623 5.4:-18907129 MS.gene98461:CDS
CATTGATATTGATCAAGGGG+AGG 0.657330 5.4:-18907061 MS.gene98461:CDS
TCTCCAGGAGATCGGTCAGG+AGG 0.664692 5.4:-18906601 MS.gene98461:CDS
TATTACGCAGAAATGGTGAA+GGG 0.681147 5.4:-18906916 MS.gene98461:CDS
GGTGAAGGGAGATAGCCACA+TGG 0.757517 5.4:-18906902 MS.gene98461:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! GATGCTATATTAGATAAACT+AGG - chr5.4:18906561-18906580 MS.gene98461:CDS 25.0%
!! TTAGTATCTCTTTTCTTTTG+CGG + chr5.4:18907149-18907168 None:intergenic 25.0%
!!! AAACAACTAATGATTTTGGT+GGG - chr5.4:18907225-18907244 MS.gene98461:CDS 25.0%
ACATTGAATCTGTTAAGAAG+TGG - chr5.4:18906952-18906971 MS.gene98461:CDS 30.0%
AGTCACAGAAATTGAAAGAA+AGG - chr5.4:18906910-18906929 MS.gene98461:CDS 30.0%
GATACTAAGAATGCCAAATT+TGG - chr5.4:18907161-18907180 MS.gene98461:CDS 30.0%
GTCACAGAAATTGAAAGAAA+GGG - chr5.4:18906911-18906930 MS.gene98461:CDS 30.0%
TCTCCATTGATATTGATCAA+GGG - chr5.4:18906625-18906644 MS.gene98461:CDS 30.0%
TCTTCTGAATCTGATTCAAA+CGG + chr5.4:18906495-18906514 None:intergenic 30.0%
! GAAACAACTAATGATTTTGG+TGG - chr5.4:18907224-18907243 MS.gene98461:CDS 30.0%
! GCTGAAACAACTAATGATTT+TGG - chr5.4:18907221-18907240 MS.gene98461:CDS 30.0%
!! AAGAATGCCAAATTTGGTTT+TGG - chr5.4:18907167-18907186 MS.gene98461:CDS 30.0%
!!! TTATTCACTGCAGTTTTAGA+TGG + chr5.4:18906537-18906556 None:intergenic 30.0%
ACATGGAAAAGGTGAAAAGT+CGG - chr5.4:18906805-18906824 MS.gene98461:CDS 35.0%
AGATAAGTCTTTGAGCTTTG+AGG - chr5.4:18907022-18907041 MS.gene98461:CDS 35.0%
AGCAAAGAGATTGTCTAAAG+AGG - chr5.4:18906884-18906903 MS.gene98461:CDS 35.0%
ATAAACTAGGAGACATAAGC+TGG - chr5.4:18906574-18906593 MS.gene98461:CDS 35.0%
CTCCATTGATATTGATCAAG+GGG - chr5.4:18906626-18906645 MS.gene98461:CDS 35.0%
CTCTCCATTGATATTGATCA+AGG - chr5.4:18906624-18906643 MS.gene98461:CDS 35.0%
TATTACGCAGAAATGGTGAA+GGG - chr5.4:18906774-18906793 MS.gene98461:CDS 35.0%
TCAAAGCTCAAAGACTTATC+TGG + chr5.4:18907023-18907042 None:intergenic 35.0%
TCGAGAGATCAAAGAAAAAG+AGG - chr5.4:18907057-18907076 MS.gene98461:CDS 35.0%
TGAAACTGAAGATTTGGATG+AGG - chr5.4:18906461-18906480 MS.gene98461:CDS 35.0%
TTGAATCTGTTAAGAAGTGG+AGG - chr5.4:18906955-18906974 MS.gene98461:CDS 35.0%
! AATGTCATCCTTTTTCTGCT+TGG + chr5.4:18906937-18906956 None:intergenic 35.0%
!!! AATGCCAAATTTGGTTTTGG+AGG - chr5.4:18907170-18907189 MS.gene98461:CDS 35.0%
AACGGTGATCCAATGATTGA+CGG - chr5.4:18906438-18906457 MS.gene98461:CDS 40.0%
AGATCAAAGAAAAAGAGGCC+CGG - chr5.4:18907062-18907081 MS.gene98461:CDS 40.0%
AGTAGATGTGAACGATGACT+TGG - chr5.4:18906653-18906672 MS.gene98461:CDS 40.0%
ATTAGATGAGAAGCGGAAGA+TGG - chr5.4:18906830-18906849 MS.gene98461:CDS 40.0%
ATTTCTGCGTAATAGTCAGC+AGG + chr5.4:18906768-18906787 None:intergenic 40.0%
CATTGATATTGATCAAGGGG+AGG - chr5.4:18906629-18906648 MS.gene98461:CDS 40.0%
CTATTACGCAGAAATGGTGA+AGG - chr5.4:18906773-18906792 MS.gene98461:CDS 40.0%
CTCCCCTTGATCAATATCAA+TGG + chr5.4:18906631-18906650 None:intergenic 40.0%
TCAGTTTCACCGTCAATCAT+TGG + chr5.4:18906450-18906469 None:intergenic 40.0%
TGACGGTGAAACTGAAGATT+TGG - chr5.4:18906455-18906474 MS.gene98461:CDS 40.0%
TTTGAGAAGCTTGAGTCGAT+GGG - chr5.4:18906726-18906745 MS.gene98461:CDS 40.0%
! ATCATTTTACACGCAGGCAT+TGG - chr5.4:18906686-18906705 MS.gene98461:CDS 40.0%
! CAAAGACTTATCTGGAGCAT+CGG + chr5.4:18907015-18907034 None:intergenic 40.0%
! GACTTTTCACCTTTTCCATG+TGG + chr5.4:18906806-18906825 None:intergenic 40.0%
! TGAGCTATCATTTTACACGC+AGG - chr5.4:18906680-18906699 MS.gene98461:CDS 40.0%
! TGCTGACTATTACGCAGAAA+TGG - chr5.4:18906767-18906786 MS.gene98461:CDS 40.0%
!! AAGTCTTTGAGCTTTGAGGA+TGG - chr5.4:18907026-18907045 MS.gene98461:CDS 40.0%
!! ATGGAGGTGTCTTTGGTTAA+CGG - chr5.4:18906420-18906439 MS.gene98461:CDS 40.0%
!!! GATTTTGGTGGGTTCAGTAA+GGG - chr5.4:18907236-18907255 MS.gene98461:CDS 40.0%
!!! TGATTTTGGTGGGTTCAGTA+AGG - chr5.4:18907235-18907254 MS.gene98461:CDS 40.0%
AGAAAGGGCCAAGCAGAAAA+AGG - chr5.4:18906926-18906945 MS.gene98461:CDS 45.0%
AGACAATCTCTTTGCTTCCC+TGG + chr5.4:18906880-18906899 None:intergenic 45.0%
AGGTAAGGCAAAGCAAGCTA+TGG - chr5.4:18907109-18907128 MS.gene98461:CDS 45.0%
AGTCAGCAGGTCTCAAGAAA+GGG + chr5.4:18906755-18906774 None:intergenic 45.0%
GCAAAGCAAGCTATGGGAAA+GGG - chr5.4:18907116-18907135 MS.gene98461:CDS 45.0%
GGTAAGGCAAAGCAAGCTAT+GGG - chr5.4:18907110-18907129 MS.gene98461:CDS 45.0%
GTCGGCTATTAGATGAGAAG+CGG - chr5.4:18906823-18906842 MS.gene98461:CDS 45.0%
TAGTCAGCAGGTCTCAAGAA+AGG + chr5.4:18906756-18906775 None:intergenic 45.0%
TGAAGAGAGAAGAAAGGCCA+GGG - chr5.4:18906860-18906879 MS.gene98461:CDS 45.0%
TGCTTCCAATCCACATTCTC+AGG + chr5.4:18906600-18906619 None:intergenic 45.0%
! CTTTGAGAAGCTTGAGTCGA+TGG - chr5.4:18906725-18906744 MS.gene98461:CDS 45.0%
!! CATCGACTCAAGCTTCTCAA+AGG + chr5.4:18906727-18906746 None:intergenic 45.0%
!!! ATTTTACACGCAGGCATTGG+AGG - chr5.4:18906689-18906708 MS.gene98461:CDS 45.0%
!!! CCAAATTTGGTTTTGGAGGC+CGG - chr5.4:18907174-18907193 MS.gene98461:CDS 45.0%
!!! TTTTACACGCAGGCATTGGA+GGG - chr5.4:18906690-18906709 MS.gene98461:CDS 45.0%
AGATGAGAAGCGGAAGATGG+AGG - chr5.4:18906833-18906852 MS.gene98461:CDS 50.0%
AGGAAGCAGAGACAACAGAG+TGG - chr5.4:18906975-18906994 MS.gene98461:CDS 50.0%
CCGGCCTCCAAAACCAAATT+TGG + chr5.4:18907177-18907196 None:intergenic 50.0%
CTGAAGAGAGAAGAAAGGCC+AGG - chr5.4:18906859-18906878 MS.gene98461:CDS 50.0%
GGAAGCAGAGACAACAGAGT+GGG - chr5.4:18906976-18906995 MS.gene98461:CDS 50.0%
GGAGGCTGAAGAGAGAAGAA+AGG - chr5.4:18906854-18906873 MS.gene98461:CDS 50.0%
GGCAAAGCAAGCTATGGGAA+AGG - chr5.4:18907115-18907134 MS.gene98461:CDS 50.0%
GGGAGATAGCCACATGGAAA+AGG - chr5.4:18906794-18906813 MS.gene98461:CDS 50.0%
TCTGCTTCTTCGATCCCTTC+CGG + chr5.4:18907196-18907215 None:intergenic 50.0%
TTCAGTAAGGGTTCTGCTGC+TGG - chr5.4:18907248-18907267 MS.gene98461:CDS 50.0%
! CATAAGCTGGCCTGAGAATG+TGG - chr5.4:18906587-18906606 MS.gene98461:CDS 50.0%
!! CAACAGAGTGGGTTTGCTGA+CGG - chr5.4:18906987-18907006 MS.gene98461:CDS 50.0%
!!! ATTTGGTTTTGGAGGCCGGA+AGG - chr5.4:18907178-18907197 MS.gene98461:CDS 50.0%
!!! TTTGGTTTTGGAGGCCGGAA+GGG - chr5.4:18907179-18907198 MS.gene98461:CDS 50.0%
AGGAGATCGGTCAGGAGGTA+AGG - chr5.4:18907094-18907113 MS.gene98461:CDS 55.0%
CCGATCTCCTGGAGATACTC+CGG + chr5.4:18907084-18907103 None:intergenic 55.0%
CGATCTCCTGGAGATACTCC+GGG + chr5.4:18907083-18907102 None:intergenic 55.0%
GGTGAAGGGAGATAGCCACA+TGG - chr5.4:18906788-18906807 MS.gene98461:CDS 55.0%
GTATCTCCAGGAGATCGGTC+AGG - chr5.4:18907086-18907105 MS.gene98461:CDS 55.0%
TGAGAAGCGGAAGATGGAGG+AGG - chr5.4:18906836-18906855 MS.gene98461:CDS 55.0%
TTACCTCCTGACCGATCTCC+TGG + chr5.4:18907095-18907114 None:intergenic 55.0%
! CCGGAGTATCTCCAGGAGAT+CGG - chr5.4:18907081-18907100 MS.gene98461:CDS 55.0%
! GCTGGCCTGAGAATGTGGAT+TGG - chr5.4:18906592-18906611 MS.gene98461:CDS 55.0%
! GGCATTGGAGGGAACTAGAC+AGG - chr5.4:18906701-18906720 MS.gene98461:CDS 55.0%
TCTCCAGGAGATCGGTCAGG+AGG - chr5.4:18907089-18907108 MS.gene98461:CDS 60.0%
! AAGAGGCCCGGAGTATCTCC+AGG - chr5.4:18907074-18907093 MS.gene98461:CDS 60.0%


Chromosome Type Strat End Strand Name
chr5.4 gene 18906420 18907292 18906420 ID=MS.gene98461
chr5.4 mRNA 18906420 18907292 18906420 ID=MS.gene98461.t1;Parent=MS.gene98461
chr5.4 exon 18906420 18907292 18906420 ID=MS.gene98461.t1.exon1;Parent=MS.gene98461.t1
chr5.4 CDS 18906420 18907292 18906420 ID=cds.MS.gene98461.t1;Parent=MS.gene98461.t1
Gene Sequence

>MS.gene98461

ATGGAGGTGTCTTTGGTTAACGGTGATCCAATGATTGACGGTGAAACTGAAGATTTGGATGAGGAAATGTCACCGTTTGAATCAGATTCAGAAGAAGATGTCAAATTAGCTGAACCATCTAAAACTGCAGTGAATAACAGAGATGCTATATTAGATAAACTAGGAGACATAAGCTGGCCTGAGAATGTGGATTGGAAGCACAAACTCTCCATTGATATTGATCAAGGGGAGGAAGTAGATGTGAACGATGACTTGGCACGTGAGCTATCATTTTACACGCAGGCATTGGAGGGAACTAGACAGGCCTTTGAGAAGCTTGAGTCGATGGGTCTCCCTTTCTTGAGACCTGCTGACTATTACGCAGAAATGGTGAAGGGAGATAGCCACATGGAAAAGGTGAAAAGTCGGCTATTAGATGAGAAGCGGAAGATGGAGGAGGCTGAAGAGAGAAGAAAGGCCAGGGAAGCAAAGAGATTGTCTAAAGAGGTTCAGTCACAGAAATTGAAAGAAAGGGCCAAGCAGAAAAAGGATGACATTGAATCTGTTAAGAAGTGGAGGAAGCAGAGACAACAGAGTGGGTTTGCTGACGGCGCCGATGCTCCAGATAAGTCTTTGAGCTTTGAGGATGGAAAAGTGTTCGAGAGATCAAAGAAAAAGAGGCCCGGAGTATCTCCAGGAGATCGGTCAGGAGGTAAGGCAAAGCAAGCTATGGGAAAGGGAAAGAAGCCGCAAAAGAAAAGAGATACTAAGAATGCCAAATTTGGTTTTGGAGGCCGGAAGGGATCGAAGAAGCAGAATACAGCTGAAACAACTAATGATTTTGGTGGGTTCAGTAAGGGTTCTGCTGCTGGAAATAAGAAGAGAAAGAGATAA

Protein sequence

>MS.gene98461.t1

MEVSLVNGDPMIDGETEDLDEEMSPFESDSEEDVKLAEPSKTAVNNRDAILDKLGDISWPENVDWKHKLSIDIDQGEEVDVNDDLARELSFYTQALEGTRQAFEKLESMGLPFLRPADYYAEMVKGDSHMEKVKSRLLDEKRKMEEAEERRKAREAKRLSKEVQSQKLKERAKQKKDDIESVKKWRKQRQQSGFADGADAPDKSLSFEDGKVFERSKKKRPGVSPGDRSGGKAKQAMGKGKKPQKKRDTKNAKFGFGGRKGSKKQNTAETTNDFGGFSKGSAAGNKKRKR