AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene69611


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene69611.t1 MTR_5g028350 97.232 289 8 0 1 289 1 289 0.0 568
MS.gene69611.t1 MTR_8g075400 76.552 290 37 4 1 289 14 273 2.26e-139 394
MS.gene69611.t1 MTR_8g075400 87.500 224 26 1 1 224 1 222 6.17e-130 369
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene69611.t1 AT3G22660 65.529 293 86 6 2 290 12 293 3.65e-126 361

Find 71 sgRNAs with CRISPR-Local

Find 89 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCGGCCTCCAAAACCAAATT+TGG 0.189093 5.3:+17537866 None:intergenic
GCTGAAACAACTAATGATTT+TGG 0.204488 5.3:-17537819 MS.gene69611:CDS
GATACTAAGAATGCCAAATT+TGG 0.209577 5.3:-17537879 MS.gene69611:CDS
AAGAATGCCAAATTTGGTTT+TGG 0.274042 5.3:-17537873 MS.gene69611:CDS
ATGGAGGTGCCTTTGATTAA+TGG 0.295822 5.3:-17538620 MS.gene69611:CDS
TCAAAGCTCAAAGACTTATC+TGG 0.326167 5.3:+17538020 None:intergenic
AGAAAGGGCCAAGCAGAAAA+AGG 0.351345 5.3:-17538114 MS.gene69611:CDS
TCAGTTTCACCGTCAATCAT+TGG 0.370634 5.3:+17538593 None:intergenic
TCTGCTTCTTCGATCCCTTC+CGG 0.371874 5.3:+17537847 None:intergenic
TGCTTCCAATCCACATTCTC+AGG 0.372391 5.3:+17538443 None:intergenic
TGACGGTGAAACTGAAGATT+TGG 0.372926 5.3:-17538585 MS.gene69611:CDS
TTCAGTAAGGGTTCTGCTGC+TGG 0.381325 5.3:-17537792 MS.gene69611:CDS
TTACCTCCTGACCGATCTCC+TGG 0.396701 5.3:+17537948 None:intergenic
AGACAATCTCTTTGCTTCCC+TGG 0.401049 5.3:+17538163 None:intergenic
AGATAAGTCTTTGAGCTTTG+AGG 0.404345 5.3:-17538018 MS.gene69611:CDS
CTGAAGAGAGAAGAAAGGCC+AGG 0.422247 5.3:-17538181 MS.gene69611:CDS
CTCTCCATTGATATTGATCA+AGG 0.429280 5.3:-17538416 MS.gene69611:CDS
TCTTCTGAATCTGATTCAAA+TGG 0.456186 5.3:+17538548 None:intergenic
AGTCACAGAAATTGAAAGAA+AGG 0.458750 5.3:-17538130 MS.gene69611:CDS
TAGTCAGCAGGTCTCAAGAA+AGG 0.478435 5.3:+17538287 None:intergenic
CTTTGAGAAGCTTGAGTCGA+TGG 0.481104 5.3:-17538315 MS.gene69611:CDS
TGCTGACTATTACGCAGAAA+TGG 0.488469 5.3:-17538273 MS.gene69611:CDS
CTCCCCTTGATCAATATCAA+TGG 0.492911 5.3:+17538412 None:intergenic
CCGATCTCCTGGAGATACTC+CGG 0.494759 5.3:+17537959 None:intergenic
AGGAGATCGGTCAGGAGGTA+AGG 0.497659 5.3:-17537946 MS.gene69611:CDS
AGTCTTTGAGCTTTGAGGAT+GGG 0.501140 5.3:-17538013 MS.gene69611:CDS
AGTCAGCAGGTCTCAAGAAA+GGG 0.501463 5.3:+17538288 None:intergenic
GGGAGATAGCCACATGGAAA+AGG 0.502899 5.3:-17538246 MS.gene69611:CDS
CATAAGCTGGCCTGAGAATG+TGG 0.503617 5.3:-17538453 MS.gene69611:CDS
GTCACAGAAATTGAAAGAAA+GGG 0.504083 5.3:-17538129 MS.gene69611:CDS
GCAAAGCAAGCTATGGGAAA+GGG 0.507585 5.3:-17537924 MS.gene69611:CDS
ATTAGATGAGAAGCGGAAGA+TGG 0.510158 5.3:-17538210 MS.gene69611:CDS
GGCATTGGAGGGAACTAGAC+AGG 0.514311 5.3:-17538339 MS.gene69611:CDS
AGGTAAGGCAAAGCAAGCTA+TGG 0.517763 5.3:-17537931 MS.gene69611:CDS
TTTGAGAAGCTTGAGTCGAT+GGG 0.525398 5.3:-17538314 MS.gene69611:CDS
GGAGGCTGAAGAGAGAAGAA+AGG 0.530598 5.3:-17538186 MS.gene69611:CDS
CAAAGACTTATCTGGAGCAT+CGG 0.536868 5.3:+17538028 None:intergenic
AGATGAGAAGCGGAAGATGG+AGG 0.539024 5.3:-17538207 MS.gene69611:CDS
GATGCTATATTAGATAAACT+TGG 0.546950 5.3:-17538479 MS.gene69611:CDS
AGCAAAGAGATTGTCTAAAG+AGG 0.557828 5.3:-17538156 MS.gene69611:CDS
AAGAGGCCCGGAGTATCTCC+AGG 0.558097 5.3:-17537966 MS.gene69611:CDS
CTCCATTGATATTGATCAAG+GGG 0.559298 5.3:-17538414 MS.gene69611:CDS
GTATCTCCAGGAGATCGGTC+AGG 0.562327 5.3:-17537954 MS.gene69611:CDS
CGATCTCCTGGAGATACTCC+GGG 0.563080 5.3:+17537960 None:intergenic
AGTAGATGTGAACGATGACT+TGG 0.565329 5.3:-17538387 MS.gene69611:CDS
ATTTCTGCGTAATAGTCAGC+AGG 0.568461 5.3:+17538275 None:intergenic
ATAAACTTGGAGACATAAGC+TGG 0.571619 5.3:-17538466 MS.gene69611:CDS
TGAAACTGAAGATTTGGATG+AGG 0.572001 5.3:-17538579 MS.gene69611:CDS
CCGGAGTATCTCCAGGAGAT+CGG 0.576336 5.3:-17537959 MS.gene69611:CDS
AAGTCTTTGAGCTTTGAGGA+TGG 0.577328 5.3:-17538014 MS.gene69611:CDS
TGAAGAGAGAAGAAAGGCCA+GGG 0.579670 5.3:-17538180 MS.gene69611:CDS
CATCGACTCAAGCTTCTCAA+AGG 0.582387 5.3:+17538316 None:intergenic
GGCAAAGCAAGCTATGGGAA+AGG 0.584114 5.3:-17537925 MS.gene69611:CDS
ATTGGATCACCATTAATCAA+AGG 0.591452 5.3:+17538611 None:intergenic
TCTCCATTGATATTGATCAA+GGG 0.594778 5.3:-17538415 MS.gene69611:CDS
AATGGTGATCCAATGATTGA+CGG 0.595326 5.3:-17538602 MS.gene69611:CDS
GGTAAGGCAAAGCAAGCTAT+GGG 0.598074 5.3:-17537930 MS.gene69611:CDS
GGAAGCAGAGACAACAGAGT+GGG 0.599709 5.3:-17538064 MS.gene69611:CDS
ACATTGAATCTGTTAAGAAG+TGG 0.600860 5.3:-17538088 MS.gene69611:CDS
TGAGAAGCGGAAGATGGAGG+AGG 0.603783 5.3:-17538204 MS.gene69611:CDS
TTGAATCTGTTAAGAAGTGG+AGG 0.608378 5.3:-17538085 MS.gene69611:CDS
CTATTACGCAGAAATGGTGA+AGG 0.608653 5.3:-17538267 MS.gene69611:CDS
GTCGGCTATTAGATGAGAAG+CGG 0.609331 5.3:-17538217 MS.gene69611:CDS
GCTGGCCTGAGAATGTGGAT+TGG 0.611875 5.3:-17538448 MS.gene69611:CDS
ACATGGAAAAGGTGAAAAGT+CGG 0.619320 5.3:-17538235 MS.gene69611:CDS
CAACAGAGTGGGTTTGCTGA+CGG 0.625190 5.3:-17538053 MS.gene69611:CDS
AGGAAGCAGAGACAACAGAG+TGG 0.651328 5.3:-17538065 MS.gene69611:CDS
CATTGATATTGATCAAGGGG+AGG 0.657330 5.3:-17538411 MS.gene69611:CDS
TCTCCAGGAGATCGGTCAGG+AGG 0.664692 5.3:-17537951 MS.gene69611:CDS
TATTACGCAGAAATGGTGAA+GGG 0.681147 5.3:-17538266 MS.gene69611:CDS
GGTGAAGGGAGATAGCCACA+TGG 0.757517 5.3:-17538252 MS.gene69611:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! GATGCTATATTAGATAAACT+TGG - chr5.3:17537911-17537930 MS.gene69611:CDS 25.0%
!! TTAGTATCTCTTTTCTTTTG+CGG + chr5.3:17538499-17538518 None:intergenic 25.0%
ACATTGAATCTGTTAAGAAG+TGG - chr5.3:17538302-17538321 MS.gene69611:CDS 30.0%
AGTCACAGAAATTGAAAGAA+AGG - chr5.3:17538260-17538279 MS.gene69611:CDS 30.0%
ATTGGATCACCATTAATCAA+AGG + chr5.3:17537782-17537801 None:intergenic 30.0%
GATACTAAGAATGCCAAATT+TGG - chr5.3:17538511-17538530 MS.gene69611:CDS 30.0%
GTCACAGAAATTGAAAGAAA+GGG - chr5.3:17538261-17538280 MS.gene69611:CDS 30.0%
TCTCCATTGATATTGATCAA+GGG - chr5.3:17537975-17537994 MS.gene69611:CDS 30.0%
TCTTCTGAATCTGATTCAAA+TGG + chr5.3:17537845-17537864 None:intergenic 30.0%
! GAAACAACTAATGATTTTGG+CGG - chr5.3:17538574-17538593 MS.gene69611:CDS 30.0%
! GCTGAAACAACTAATGATTT+TGG - chr5.3:17538571-17538590 MS.gene69611:CDS 30.0%
!! AAACAACTAATGATTTTGGC+GGG - chr5.3:17538575-17538594 MS.gene69611:CDS 30.0%
!! AAGAATGCCAAATTTGGTTT+TGG - chr5.3:17538517-17538536 MS.gene69611:CDS 30.0%
!!! TTATTCACTGCAGTTTTAGA+TGG + chr5.3:17537887-17537906 None:intergenic 30.0%
AATGGTGATCCAATGATTGA+CGG - chr5.3:17537788-17537807 MS.gene69611:CDS 35.0%
ACATGGAAAAGGTGAAAAGT+CGG - chr5.3:17538155-17538174 MS.gene69611:CDS 35.0%
AGATAAGTCTTTGAGCTTTG+AGG - chr5.3:17538372-17538391 MS.gene69611:CDS 35.0%
AGCAAAGAGATTGTCTAAAG+AGG - chr5.3:17538234-17538253 MS.gene69611:CDS 35.0%
ATAAACTTGGAGACATAAGC+TGG - chr5.3:17537924-17537943 MS.gene69611:CDS 35.0%
CTCCATTGATATTGATCAAG+GGG - chr5.3:17537976-17537995 MS.gene69611:CDS 35.0%
CTCTCCATTGATATTGATCA+AGG - chr5.3:17537974-17537993 MS.gene69611:CDS 35.0%
TATTACGCAGAAATGGTGAA+GGG - chr5.3:17538124-17538143 MS.gene69611:CDS 35.0%
TCAAAGCTCAAAGACTTATC+TGG + chr5.3:17538373-17538392 None:intergenic 35.0%
TCGAGAGATCAAAGAAAAAG+AGG - chr5.3:17538407-17538426 MS.gene69611:CDS 35.0%
TGAAACTGAAGATTTGGATG+AGG - chr5.3:17537811-17537830 MS.gene69611:CDS 35.0%
TTGAATCTGTTAAGAAGTGG+AGG - chr5.3:17538305-17538324 MS.gene69611:CDS 35.0%
! AATGTCATCCTTTTTCTGCT+TGG + chr5.3:17538287-17538306 None:intergenic 35.0%
!!! AATGCCAAATTTGGTTTTGG+AGG - chr5.3:17538520-17538539 MS.gene69611:CDS 35.0%
AGATCAAAGAAAAAGAGGCC+CGG - chr5.3:17538412-17538431 MS.gene69611:CDS 40.0%
AGTAGATGTGAACGATGACT+TGG - chr5.3:17538003-17538022 MS.gene69611:CDS 40.0%
ATTAGATGAGAAGCGGAAGA+TGG - chr5.3:17538180-17538199 MS.gene69611:CDS 40.0%
ATTTCTGCGTAATAGTCAGC+AGG + chr5.3:17538118-17538137 None:intergenic 40.0%
CATTGATATTGATCAAGGGG+AGG - chr5.3:17537979-17537998 MS.gene69611:CDS 40.0%
CTATTACGCAGAAATGGTGA+AGG - chr5.3:17538123-17538142 MS.gene69611:CDS 40.0%
CTCCCCTTGATCAATATCAA+TGG + chr5.3:17537981-17538000 None:intergenic 40.0%
TCAGTTTCACCGTCAATCAT+TGG + chr5.3:17537800-17537819 None:intergenic 40.0%
TGACGGTGAAACTGAAGATT+TGG - chr5.3:17537805-17537824 MS.gene69611:CDS 40.0%
TTTGAGAAGCTTGAGTCGAT+GGG - chr5.3:17538076-17538095 MS.gene69611:CDS 40.0%
! ATCATTTTACACGCAGGCAT+TGG - chr5.3:17538036-17538055 MS.gene69611:CDS 40.0%
! CAAAGACTTATCTGGAGCAT+CGG + chr5.3:17538365-17538384 None:intergenic 40.0%
! GACTTTTCACCTTTTCCATG+TGG + chr5.3:17538156-17538175 None:intergenic 40.0%
! TGAGCTATCATTTTACACGC+AGG - chr5.3:17538030-17538049 MS.gene69611:CDS 40.0%
! TGCTGACTATTACGCAGAAA+TGG - chr5.3:17538117-17538136 MS.gene69611:CDS 40.0%
!! AAGTCTTTGAGCTTTGAGGA+TGG - chr5.3:17538376-17538395 MS.gene69611:CDS 40.0%
!! AGTCTTTGAGCTTTGAGGAT+GGG - chr5.3:17538377-17538396 MS.gene69611:CDS 40.0%
!! ATGGAGGTGCCTTTGATTAA+TGG - chr5.3:17537770-17537789 MS.gene69611:CDS 40.0%
AGAAAGGGCCAAGCAGAAAA+AGG - chr5.3:17538276-17538295 MS.gene69611:CDS 45.0%
AGACAATCTCTTTGCTTCCC+TGG + chr5.3:17538230-17538249 None:intergenic 45.0%
AGGTAAGGCAAAGCAAGCTA+TGG - chr5.3:17538459-17538478 MS.gene69611:CDS 45.0%
AGTCAGCAGGTCTCAAGAAA+GGG + chr5.3:17538105-17538124 None:intergenic 45.0%
GCAAAGCAAGCTATGGGAAA+GGG - chr5.3:17538466-17538485 MS.gene69611:CDS 45.0%
GGTAAGGCAAAGCAAGCTAT+GGG - chr5.3:17538460-17538479 MS.gene69611:CDS 45.0%
GTCGGCTATTAGATGAGAAG+CGG - chr5.3:17538173-17538192 MS.gene69611:CDS 45.0%
TAGTCAGCAGGTCTCAAGAA+AGG + chr5.3:17538106-17538125 None:intergenic 45.0%
TGAAGAGAGAAGAAAGGCCA+GGG - chr5.3:17538210-17538229 MS.gene69611:CDS 45.0%
TGCTTCCAATCCACATTCTC+AGG + chr5.3:17537950-17537969 None:intergenic 45.0%
! CTTTGAGAAGCTTGAGTCGA+TGG - chr5.3:17538075-17538094 MS.gene69611:CDS 45.0%
!! CATCGACTCAAGCTTCTCAA+AGG + chr5.3:17538077-17538096 None:intergenic 45.0%
!!! ATTTTACACGCAGGCATTGG+AGG - chr5.3:17538039-17538058 MS.gene69611:CDS 45.0%
!!! CCAAATTTGGTTTTGGAGGC+CGG - chr5.3:17538524-17538543 MS.gene69611:CDS 45.0%
!!! GATTTTGGCGGGTTCAGTAA+GGG - chr5.3:17538586-17538605 MS.gene69611:CDS 45.0%
!!! TGATTTTGGCGGGTTCAGTA+AGG - chr5.3:17538585-17538604 MS.gene69611:CDS 45.0%
!!! TTTTACACGCAGGCATTGGA+GGG - chr5.3:17538040-17538059 MS.gene69611:CDS 45.0%
AGATGAGAAGCGGAAGATGG+AGG - chr5.3:17538183-17538202 MS.gene69611:CDS 50.0%
AGGAAGCAGAGACAACAGAG+TGG - chr5.3:17538325-17538344 MS.gene69611:CDS 50.0%
CCGGCCTCCAAAACCAAATT+TGG + chr5.3:17538527-17538546 None:intergenic 50.0%
CTGAAGAGAGAAGAAAGGCC+AGG - chr5.3:17538209-17538228 MS.gene69611:CDS 50.0%
GGAAGCAGAGACAACAGAGT+GGG - chr5.3:17538326-17538345 MS.gene69611:CDS 50.0%
GGAGGCTGAAGAGAGAAGAA+AGG - chr5.3:17538204-17538223 MS.gene69611:CDS 50.0%
GGCAAAGCAAGCTATGGGAA+AGG - chr5.3:17538465-17538484 MS.gene69611:CDS 50.0%
GGGAGATAGCCACATGGAAA+AGG - chr5.3:17538144-17538163 MS.gene69611:CDS 50.0%
TCTGCTTCTTCGATCCCTTC+CGG + chr5.3:17538546-17538565 None:intergenic 50.0%
TTCAGTAAGGGTTCTGCTGC+TGG - chr5.3:17538598-17538617 MS.gene69611:CDS 50.0%
! CATAAGCTGGCCTGAGAATG+TGG - chr5.3:17537937-17537956 MS.gene69611:CDS 50.0%
!! CAACAGAGTGGGTTTGCTGA+CGG - chr5.3:17538337-17538356 MS.gene69611:CDS 50.0%
!!! ATTTGGTTTTGGAGGCCGGA+AGG - chr5.3:17538528-17538547 MS.gene69611:CDS 50.0%
!!! TTTGGTTTTGGAGGCCGGAA+GGG - chr5.3:17538529-17538548 MS.gene69611:CDS 50.0%
AGGAGATCGGTCAGGAGGTA+AGG - chr5.3:17538444-17538463 MS.gene69611:CDS 55.0%
CCGATCTCCTGGAGATACTC+CGG + chr5.3:17538434-17538453 None:intergenic 55.0%
CGATCTCCTGGAGATACTCC+GGG + chr5.3:17538433-17538452 None:intergenic 55.0%
GGTGAAGGGAGATAGCCACA+TGG - chr5.3:17538138-17538157 MS.gene69611:CDS 55.0%
GTATCTCCAGGAGATCGGTC+AGG - chr5.3:17538436-17538455 MS.gene69611:CDS 55.0%
TGAGAAGCGGAAGATGGAGG+AGG - chr5.3:17538186-17538205 MS.gene69611:CDS 55.0%
TTACCTCCTGACCGATCTCC+TGG + chr5.3:17538445-17538464 None:intergenic 55.0%
! CCGGAGTATCTCCAGGAGAT+CGG - chr5.3:17538431-17538450 MS.gene69611:CDS 55.0%
! GCTGGCCTGAGAATGTGGAT+TGG - chr5.3:17537942-17537961 MS.gene69611:CDS 55.0%
! GGCATTGGAGGGAACTAGAC+AGG - chr5.3:17538051-17538070 MS.gene69611:CDS 55.0%
TCTCCAGGAGATCGGTCAGG+AGG - chr5.3:17538439-17538458 MS.gene69611:CDS 60.0%
! AAGAGGCCCGGAGTATCTCC+AGG - chr5.3:17538424-17538443 MS.gene69611:CDS 60.0%


Chromosome Type Strat End Strand Name
chr5.3 gene 17537770 17538642 17537770 ID=MS.gene69611
chr5.3 mRNA 17537770 17538642 17537770 ID=MS.gene69611.t1;Parent=MS.gene69611
chr5.3 exon 17537770 17538642 17537770 ID=MS.gene69611.t1.exon1;Parent=MS.gene69611.t1
chr5.3 CDS 17537770 17538642 17537770 ID=cds.MS.gene69611.t1;Parent=MS.gene69611.t1
Gene Sequence

>MS.gene69611

ATGGAGGTGCCTTTGATTAATGGTGATCCAATGATTGACGGTGAAACTGAAGATTTGGATGAGGAAATGTCACCATTTGAATCAGATTCAGAAGAAGATGTCAAATTAGCTGAACCATCTAAAACTGCAGTGAATAACAGAGATGCTATATTAGATAAACTTGGAGACATAAGCTGGCCTGAGAATGTGGATTGGAAGCACAAACTCTCCATTGATATTGATCAAGGGGAGGAAGTAGATGTGAACGATGACTTGGCACGTGAGCTATCATTTTACACGCAGGCATTGGAGGGAACTAGACAGGCCTTTGAGAAGCTTGAGTCGATGGGTCTCCCTTTCTTGAGACCTGCTGACTATTACGCAGAAATGGTGAAGGGAGATAGCCACATGGAAAAGGTGAAAAGTCGGCTATTAGATGAGAAGCGGAAGATGGAGGAGGCTGAAGAGAGAAGAAAGGCCAGGGAAGCAAAGAGATTGTCTAAAGAGGTTCAGTCACAGAAATTGAAAGAAAGGGCCAAGCAGAAAAAGGATGACATTGAATCTGTTAAGAAGTGGAGGAAGCAGAGACAACAGAGTGGGTTTGCTGACGGCGCCGATGCTCCAGATAAGTCTTTGAGCTTTGAGGATGGGAAAGTGTTCGAGAGATCAAAGAAAAAGAGGCCCGGAGTATCTCCAGGAGATCGGTCAGGAGGTAAGGCAAAGCAAGCTATGGGAAAGGGAAAGAAGCCGCAAAAGAAAAGAGATACTAAGAATGCCAAATTTGGTTTTGGAGGCCGGAAGGGATCGAAGAAGCAGAATACAGCTGAAACAACTAATGATTTTGGCGGGTTCAGTAAGGGTTCTGCTGCTGGAAATAAGAAGAGAAAGAGATAA

Protein sequence

>MS.gene69611.t1

MEVPLINGDPMIDGETEDLDEEMSPFESDSEEDVKLAEPSKTAVNNRDAILDKLGDISWPENVDWKHKLSIDIDQGEEVDVNDDLARELSFYTQALEGTRQAFEKLESMGLPFLRPADYYAEMVKGDSHMEKVKSRLLDEKRKMEEAEERRKAREAKRLSKEVQSQKLKERAKQKKDDIESVKKWRKQRQQSGFADGADAPDKSLSFEDGKVFERSKKKRPGVSPGDRSGGKAKQAMGKGKKPQKKRDTKNAKFGFGGRKGSKKQNTAETTNDFGGFSKGSAAGNKKRKR