AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene89671


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene89671.t1 MTR_5g028350 85.121 289 40 2 1 286 1 289 6.43e-169 469
MS.gene89671.t1 MTR_8g075400 86.063 287 13 2 1 287 14 273 2.94e-162 452
MS.gene89671.t1 MTR_8g075400 96.847 222 7 0 1 222 1 222 2.73e-157 439
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene89671.t1 AT3G22660 63.889 288 97 3 2 288 12 293 1.01e-124 357

Find 68 sgRNAs with CRISPR-Local

Find 82 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTGCCTCCAAAGCCAAATT+TGG 0.135328 8.3:-28188252 None:intergenic
GCTGATACTACTAATGATTT+TGG 0.192046 8.3:+28188299 MS.gene89671:CDS
GATGTTAAGAATTCCAAATT+TGG 0.278385 8.3:+28188239 MS.gene89671:CDS
ATTAGCTGAACCATCTAAAA+AGG 0.298870 8.3:+28187611 MS.gene89671:CDS
TGTATCCATTCCACATTCTC+TGG 0.362791 8.3:-28187684 None:intergenic
AATTCCAAATTTGGCTTTGG+AGG 0.373337 8.3:+28188248 MS.gene89671:CDS
AAGAATTCCAAATTTGGCTT+TGG 0.378859 8.3:+28188245 MS.gene89671:CDS
GAAAAGACCTGGAGTGTCTC+CGG 0.423295 8.3:+28188154 MS.gene89671:CDS
AGTCACAGAAATTAAAAGAA+AGG 0.442695 8.3:+28187997 MS.gene89671:CDS
GTGTCTCCGGGTGACCGATC+AGG 0.444973 8.3:+28188167 MS.gene89671:CDS
CTTTGAGAAACTTCAGTCAA+TGG 0.454306 8.3:+28187812 MS.gene89671:CDS
CGATGGCGCAGATAAGGCTT+TGG 0.456534 8.3:+28188094 MS.gene89671:CDS
CTGCTCTTGATCGATGTCAA+TGG 0.460523 8.3:-28187715 None:intergenic
GTCACAGAAATTAAAAGAAA+GGG 0.462057 8.3:+28187998 MS.gene89671:CDS
GGTAAGGCAAAGCAAGCCTT+TGG 0.462245 8.3:+28188191 MS.gene89671:CDS
TAGTCTGCAGGCCTTAGAAA+AGG 0.462682 8.3:-28187840 None:intergenic
TGGCTTCTTTCCCTTTCCAA+AGG 0.463981 8.3:-28188207 None:intergenic
TGCAGACTATTACGCTGAAA+TGG 0.466246 8.3:+28187854 MS.gene89671:CDS
ACATTGAATCTGTTAAGAAA+TGG 0.472489 8.3:+28188039 MS.gene89671:CDS
TGCTGACGATGGCGCAGATA+AGG 0.473187 8.3:+28188088 MS.gene89671:CDS
AGAGGCTGAAGAGAGAAGAA+AGG 0.475560 8.3:+28187941 MS.gene89671:CDS
TTTGAGAAACTTCAGTCAAT+GGG 0.475684 8.3:+28187813 MS.gene89671:CDS
CTGAAGAGAGAAGAAAGGGC+AGG 0.484625 8.3:+28187946 MS.gene89671:CDS
GAGGAAGTAAGAAGAGAAAG+AGG 0.489339 8.3:+28188348 MS.gene89671:CDS
GCAAAGCAAGCCTTTGGAAA+GGG 0.492131 8.3:+28188197 MS.gene89671:CDS
AATGATGATACAATGGTTGA+TGG 0.492459 8.3:+28187525 MS.gene89671:CDS
AACCTCTTTAGATAATCTCT+TGG 0.505477 8.3:-28187973 None:intergenic
TTACCTCCTGATCGGTCACC+CGG 0.505687 8.3:-28188173 None:intergenic
TTTGGCTTTGGAGGCAAGAA+GGG 0.507591 8.3:+28188257 MS.gene89671:CDS
CATAAGCTGGCCAGAGAATG+TGG 0.514747 8.3:+28187674 MS.gene89671:CDS
AGATAAGGCTTTGGACTTCG+AGG 0.515894 8.3:+28188103 MS.gene89671:CDS
AGATTGAATGGAGCTGCCTC+TGG 0.515931 8.3:+28187500 None:intergenic
GGCATTGGAGGGTACTAGAC+AGG 0.518118 8.3:+28187788 MS.gene89671:CDS
TCTTCTGATTCTGATTCAGA+TGG 0.519325 8.3:-28187579 None:intergenic
ATTTGGCTTTGGAGGCAAGA+AGG 0.519353 8.3:+28188256 MS.gene89671:CDS
GCTTTGCCTTACCTCCTGAT+CGG 0.526130 8.3:-28188181 None:intergenic
GAGGCTGAAGAGAGAAGAAA+GGG 0.527920 8.3:+28187942 MS.gene89671:CDS
GATGCTCTATTAGATAAACT+TGG 0.532375 8.3:+28187648 MS.gene89671:CDS
AAAAGACCTGGAGTGTCTCC+GGG 0.535827 8.3:+28188155 MS.gene89671:CDS
ATTAGCAGAGAAGCAGAAGA+TGG 0.537363 8.3:+28187917 MS.gene89671:CDS
AGTCTGCAGGCCTTAGAAAA+GGG 0.538151 8.3:-28187839 None:intergenic
CGGTCAAAGAAGAAAAGACC+TGG 0.554286 8.3:+28188143 MS.gene89671:CDS
ATTTCAGCGTAATAGTCTGC+AGG 0.559772 8.3:-28187852 None:intergenic
CATTGACATCGATCAAGAGC+AGG 0.572289 8.3:+28187716 MS.gene89671:CDS
TGAAGAGAGAAGAAAGGGCA+GGG 0.572509 8.3:+28187947 MS.gene89671:CDS
ATAAACTTGGAGACATAAGC+TGG 0.573791 8.3:+28187661 MS.gene89671:CDS
GGGTGACCGATCAGGAGGTA+AGG 0.574615 8.3:+28188175 MS.gene89671:CDS
CGGTCACCCGGAGACACTCC+AGG 0.576723 8.3:-28188161 None:intergenic
GGCCAAGAGATTATCTAAAG+AGG 0.579968 8.3:+28187971 MS.gene89671:CDS
GGCAAAGCAAGCCTTTGGAA+AGG 0.581107 8.3:+28188196 MS.gene89671:CDS
AGAGAAGCAGAAGATGGTAG+AGG 0.581371 8.3:+28187923 MS.gene89671:CDS
AGTAGATGTTAATGATGACT+TGG 0.588825 8.3:+28187740 MS.gene89671:CDS
TTGAATCTGTTAAGAAATGG+AGG 0.588933 8.3:+28188042 MS.gene89671:CDS
TTTGGACTTCGAGGACGGAA+AGG 0.590393 8.3:+28188112 MS.gene89671:CDS
AGGAAGCAGAGGCAACAAAG+TGG 0.602796 8.3:+28188062 MS.gene89671:CDS
AAGGCTTTGGACTTCGAGGA+CGG 0.606211 8.3:+28188107 MS.gene89671:CDS
GGAAGCAGAGGCAACAAAGT+GGG 0.615514 8.3:+28188063 MS.gene89671:CDS
CAAAGTGGGTTTGCTGACGA+TGG 0.619387 8.3:+28188077 MS.gene89671:CDS
TTAAGAAATGGAGGAAGCAG+AGG 0.621715 8.3:+28188051 MS.gene89671:CDS
CATTGACTGAAGTTTCTCAA+AGG 0.625618 8.3:-28187811 None:intergenic
TCTCCGGGTGACCGATCAGG+AGG 0.644315 8.3:+28188170 MS.gene89671:CDS
GCTGGCCAGAGAATGTGGAA+TGG 0.647233 8.3:+28187679 MS.gene89671:CDS
ATTGTATCATCATTTACCAG+AGG 0.659574 8.3:-28187516 None:intergenic
AGGACGGAAAGGCGTTCGAG+CGG 0.661497 8.3:+28188123 MS.gene89671:CDS
TCTGGTAAATGATGATACAA+TGG 0.663757 8.3:+28187518 MS.gene89671:CDS
AGAGAGAAGAAAGGGCAGGG+AGG 0.683036 8.3:+28187950 MS.gene89671:CDS
TGAAGCTGAGTATTTGAGTG+AGG 0.691291 8.3:+28187548 MS.gene89671:CDS
GGTGAAGACAGATAGCCACA+TGG 0.738846 8.3:+28187875 MS.gene89671:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! ACATTGAATCTGTTAAGAAA+TGG + chr8.3:28188039-28188058 MS.gene89671:CDS 25.0%
! AGTCACAGAAATTAAAAGAA+AGG + chr8.3:28187997-28188016 MS.gene89671:CDS 25.0%
! GATGTTAAGAATTCCAAATT+TGG + chr8.3:28188239-28188258 MS.gene89671:CDS 25.0%
! GTCACAGAAATTAAAAGAAA+GGG + chr8.3:28187998-28188017 MS.gene89671:CDS 25.0%
!! TTATTTACTGCCTTTTTAGA+TGG - chr8.3:28187624-28187643 None:intergenic 25.0%
!! TTCTTAACATCTCTTTTCTT+TGG - chr8.3:28188230-28188249 None:intergenic 25.0%
AACCTCTTTAGATAATCTCT+TGG - chr8.3:28187976-28187995 None:intergenic 30.0%
AATGATGATACAATGGTTGA+TGG + chr8.3:28187525-28187544 MS.gene89671:CDS 30.0%
ACATGGAAAAAGTGAAAAGT+CGG + chr8.3:28187892-28187911 MS.gene89671:CDS 30.0%
AGTAGATGTTAATGATGACT+TGG + chr8.3:28187740-28187759 MS.gene89671:CDS 30.0%
ATTAGCTGAACCATCTAAAA+AGG + chr8.3:28187611-28187630 MS.gene89671:CDS 30.0%
ATTGTATCATCATTTACCAG+AGG - chr8.3:28187519-28187538 None:intergenic 30.0%
GATGCTCTATTAGATAAACT+TGG + chr8.3:28187648-28187667 MS.gene89671:CDS 30.0%
TCTGGTAAATGATGATACAA+TGG + chr8.3:28187518-28187537 MS.gene89671:CDS 30.0%
TTGAATCTGTTAAGAAATGG+AGG + chr8.3:28188042-28188061 MS.gene89671:CDS 30.0%
TTTGAGAAACTTCAGTCAAT+GGG + chr8.3:28187813-28187832 MS.gene89671:CDS 30.0%
! AAGAATTCCAAATTTGGCTT+TGG + chr8.3:28188245-28188264 MS.gene89671:CDS 30.0%
! GATACTACTAATGATTTTGG+TGG + chr8.3:28188302-28188321 MS.gene89671:CDS 30.0%
! GCTGATACTACTAATGATTT+TGG + chr8.3:28188299-28188318 MS.gene89671:CDS 30.0%
ATAAACTTGGAGACATAAGC+TGG + chr8.3:28187661-28187680 MS.gene89671:CDS 35.0%
TCTTCTGATTCTGATTCAGA+TGG - chr8.3:28187582-28187601 None:intergenic 35.0%
! AATGTCGTTCTTTTTCTGCT+TGG - chr8.3:28188024-28188043 None:intergenic 35.0%
! CATTGACTGAAGTTTCTCAA+AGG - chr8.3:28187814-28187833 None:intergenic 35.0%
! CTTTGAGAAACTTCAGTCAA+TGG + chr8.3:28187812-28187831 MS.gene89671:CDS 35.0%
! TTTGGTGGCTTCAGTAAAAA+GGG + chr8.3:28188317-28188336 MS.gene89671:CDS 35.0%
!! AATTCCAAATTTGGCTTTGG+AGG + chr8.3:28188248-28188267 MS.gene89671:CDS 35.0%
!! TTTTGGTGGCTTCAGTAAAA+AGG + chr8.3:28188316-28188335 MS.gene89671:CDS 35.0%
!!! GACTTTTCACTTTTTCCATG+TGG - chr8.3:28187893-28187912 None:intergenic 35.0%
ATTAGCAGAGAAGCAGAAGA+TGG + chr8.3:28187917-28187936 MS.gene89671:CDS 40.0%
ATTTCAGCGTAATAGTCTGC+AGG - chr8.3:28187855-28187874 None:intergenic 40.0%
GAGGAAGTAAGAAGAGAAAG+AGG + chr8.3:28188348-28188367 MS.gene89671:CDS 40.0%
GGCCAAGAGATTATCTAAAG+AGG + chr8.3:28187971-28187990 MS.gene89671:CDS 40.0%
TGAAGCTGAGTATTTGAGTG+AGG + chr8.3:28187548-28187567 MS.gene89671:CDS 40.0%
TGCAGACTATTACGCTGAAA+TGG + chr8.3:28187854-28187873 MS.gene89671:CDS 40.0%
TGTATCCATTCCACATTCTC+TGG - chr8.3:28187687-28187706 None:intergenic 40.0%
TTAAGAAATGGAGGAAGCAG+AGG + chr8.3:28188051-28188070 MS.gene89671:CDS 40.0%
! AGAGCTTGCATTTTACACTC+AGG + chr8.3:28187767-28187786 MS.gene89671:CDS 40.0%
! TGCATTTTACACTCAGGCAT+TGG + chr8.3:28187773-28187792 MS.gene89671:CDS 40.0%
!! TTCAGTAAAAAGGGTGCTGT+TGG + chr8.3:28188326-28188345 MS.gene89671:CDS 40.0%
!!! ATTTTACACTCAGGCATTGG+AGG + chr8.3:28187776-28187795 MS.gene89671:CDS 40.0%
!!! TTTTACACTCAGGCATTGGA+GGG + chr8.3:28187777-28187796 MS.gene89671:CDS 40.0%
AGAGAAGCAGAAGATGGTAG+AGG + chr8.3:28187923-28187942 MS.gene89671:CDS 45.0%
AGAGGCTGAAGAGAGAAGAA+AGG + chr8.3:28187941-28187960 MS.gene89671:CDS 45.0%
AGTCTGCAGGCCTTAGAAAA+GGG - chr8.3:28187842-28187861 None:intergenic 45.0%
CATTGACATCGATCAAGAGC+AGG + chr8.3:28187716-28187735 MS.gene89671:CDS 45.0%
CGGTCAAAGAAGAAAAGACC+TGG + chr8.3:28188143-28188162 MS.gene89671:CDS 45.0%
CTGCTCTTGATCGATGTCAA+TGG - chr8.3:28187718-28187737 None:intergenic 45.0%
CTTGCCTCCAAAGCCAAATT+TGG - chr8.3:28188255-28188274 None:intergenic 45.0%
GAGGCTGAAGAGAGAAGAAA+GGG + chr8.3:28187942-28187961 MS.gene89671:CDS 45.0%
TAGTCTGCAGGCCTTAGAAA+AGG - chr8.3:28187843-28187862 None:intergenic 45.0%
TGAAGAGAGAAGAAAGGGCA+GGG + chr8.3:28187947-28187966 MS.gene89671:CDS 45.0%
TGGCTTCTTTCCCTTTCCAA+AGG - chr8.3:28188210-28188229 None:intergenic 45.0%
! CAATGGGTCTCCCTTTTCTA+AGG + chr8.3:28187829-28187848 MS.gene89671:CDS 45.0%
! GCAAAGCAAGCCTTTGGAAA+GGG + chr8.3:28188197-28188216 MS.gene89671:CDS 45.0%
!! AGATAAGGCTTTGGACTTCG+AGG + chr8.3:28188103-28188122 MS.gene89671:CDS 45.0%
!! AGTAAAAAGGGTGCTGTTGG+AGG + chr8.3:28188329-28188348 MS.gene89671:CDS 45.0%
!! ATTTGGCTTTGGAGGCAAGA+AGG + chr8.3:28188256-28188275 MS.gene89671:CDS 45.0%
!! TTTGGCTTTGGAGGCAAGAA+GGG + chr8.3:28188257-28188276 MS.gene89671:CDS 45.0%
AGGAAGCAGAGGCAACAAAG+TGG + chr8.3:28188062-28188081 MS.gene89671:CDS 50.0%
CATAAGCTGGCCAGAGAATG+TGG + chr8.3:28187674-28187693 MS.gene89671:CDS 50.0%
CTGAAGAGAGAAGAAAGGGC+AGG + chr8.3:28187946-28187965 MS.gene89671:CDS 50.0%
GCTTTGCCTTACCTCCTGAT+CGG - chr8.3:28188184-28188203 None:intergenic 50.0%
GGAAGCAGAGGCAACAAAGT+GGG + chr8.3:28188063-28188082 MS.gene89671:CDS 50.0%
GGTAAGGCAAAGCAAGCCTT+TGG + chr8.3:28188191-28188210 MS.gene89671:CDS 50.0%
GGTGAAGACAGATAGCCACA+TGG + chr8.3:28187875-28187894 MS.gene89671:CDS 50.0%
TTTGGACTTCGAGGACGGAA+AGG + chr8.3:28188112-28188131 MS.gene89671:CDS 50.0%
! AAAAGACCTGGAGTGTCTCC+GGG + chr8.3:28188155-28188174 MS.gene89671:CDS 50.0%
! GAAAAGACCTGGAGTGTCTC+CGG + chr8.3:28188154-28188173 MS.gene89671:CDS 50.0%
! GGCAAAGCAAGCCTTTGGAA+AGG + chr8.3:28188196-28188215 MS.gene89671:CDS 50.0%
!! AAGGCTTTGGACTTCGAGGA+CGG + chr8.3:28188107-28188126 MS.gene89671:CDS 50.0%
!! CAAAGTGGGTTTGCTGACGA+TGG + chr8.3:28188077-28188096 MS.gene89671:CDS 50.0%
AGAGAGAAGAAAGGGCAGGG+AGG + chr8.3:28187950-28187969 MS.gene89671:CDS 55.0%
CGATGGCGCAGATAAGGCTT+TGG + chr8.3:28188094-28188113 MS.gene89671:CDS 55.0%
GCTGGCCAGAGAATGTGGAA+TGG + chr8.3:28187679-28187698 MS.gene89671:CDS 55.0%
TGCTGACGATGGCGCAGATA+AGG + chr8.3:28188088-28188107 MS.gene89671:CDS 55.0%
TTACCTCCTGATCGGTCACC+CGG - chr8.3:28188176-28188195 None:intergenic 55.0%
!! GGCATTGGAGGGTACTAGAC+AGG + chr8.3:28187788-28187807 MS.gene89671:CDS 55.0%
GGGTGACCGATCAGGAGGTA+AGG + chr8.3:28188175-28188194 MS.gene89671:CDS 60.0%
!! AGGACGGAAAGGCGTTCGAG+CGG + chr8.3:28188123-28188142 MS.gene89671:CDS 60.0%
GTGTCTCCGGGTGACCGATC+AGG + chr8.3:28188167-28188186 MS.gene89671:CDS 65.0%
TCTCCGGGTGACCGATCAGG+AGG + chr8.3:28188170-28188189 MS.gene89671:CDS 65.0%
CGGTCACCCGGAGACACTCC+AGG - chr8.3:28188164-28188183 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr8.3 gene 28187507 28188373 28187507 ID=MS.gene89671
chr8.3 mRNA 28187507 28188373 28187507 ID=MS.gene89671.t1;Parent=MS.gene89671
chr8.3 exon 28187507 28188373 28187507 ID=MS.gene89671.t1.exon1;Parent=MS.gene89671.t1
chr8.3 CDS 28187507 28188373 28187507 ID=cds.MS.gene89671.t1;Parent=MS.gene89671.t1
Gene Sequence

>MS.gene89671

ATGGAGCTGCCTCTGGTAAATGATGATACAATGGTTGATGGTGAAGCTGAGTATTTGAGTGAGGAAATGTCACCATCTGAATCAGAATCAGAAGAAGATGTGAAATTAGCTGAACCATCTAAAAAGGCAGTAAATAACAGAGATGCTCTATTAGATAAACTTGGAGACATAAGCTGGCCAGAGAATGTGGAATGGATACACAAACTCTCCATTGACATCGATCAAGAGCAGGAAGTAGATGTTAATGATGACTTGGCACGAGAGCTTGCATTTTACACTCAGGCATTGGAGGGTACTAGACAGGCCTTTGAGAAACTTCAGTCAATGGGTCTCCCTTTTCTAAGGCCTGCAGACTATTACGCTGAAATGGTGAAGACAGATAGCCACATGGAAAAAGTGAAAAGTCGGCTATTAGCAGAGAAGCAGAAGATGGTAGAGGCTGAAGAGAGAAGAAAGGGCAGGGAGGCCAAGAGATTATCTAAAGAGGTTCAGTCACAGAAATTAAAAGAAAGGGCCAAGCAGAAAAAGAACGACATTGAATCTGTTAAGAAATGGAGGAAGCAGAGGCAACAAAGTGGGTTTGCTGACGATGGCGCAGATAAGGCTTTGGACTTCGAGGACGGAAAGGCGTTCGAGCGGTCAAAGAAGAAAAGACCTGGAGTGTCTCCGGGTGACCGATCAGGAGGTAAGGCAAAGCAAGCCTTTGGAAAGGGAAAGAAGCCAAAGAAAAGAGATGTTAAGAATTCCAAATTTGGCTTTGGAGGCAAGAAGGGTTTGAAGAAGCAGAATACAGCTGATACTACTAATGATTTTGGTGGCTTCAGTAAAAAGGGTGCTGTTGGAGGAAGTAAGAAGAGAAAGAGGTAA

Protein sequence

>MS.gene89671.t1

MELPLVNDDTMVDGEAEYLSEEMSPSESESEEDVKLAEPSKKAVNNRDALLDKLGDISWPENVEWIHKLSIDIDQEQEVDVNDDLARELAFYTQALEGTRQAFEKLQSMGLPFLRPADYYAEMVKTDSHMEKVKSRLLAEKQKMVEAEERRKGREAKRLSKEVQSQKLKERAKQKKNDIESVKKWRKQRQQSGFADDGADKALDFEDGKAFERSKKKRPGVSPGDRSGGKAKQAFGKGKKPKKRDVKNSKFGFGGKKGLKKQNTADTTNDFGGFSKKGAVGGSKKRKR