Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene89545.t1 | XP_013468423.1 | 100 | 182 | 0 | 0 | 1 | 182 | 1 | 182 | 1.60E-98 | 368.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene89545.t1 | Q0JG75 | 64.4 | 180 | 61 | 2 | 2 | 181 | 3 | 179 | 3.0e-56 | 219.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene89545.t1 | A0A072VWK2 | 100.0 | 182 | 0 | 0 | 1 | 182 | 1 | 182 | 1.2e-98 | 368.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049171 | MS.gene89545 | 0.888776 | 4.37E-73 | -1.69E-46 |
| MS.gene049173 | MS.gene89545 | 0.876582 | 1.24E-68 | -1.69E-46 |
| MS.gene049217 | MS.gene89545 | 0.86509 | 7.60E-65 | -1.69E-46 |
| MS.gene049222 | MS.gene89545 | 0.865265 | 6.69E-65 | -1.69E-46 |
| MS.gene049232 | MS.gene89545 | 0.823736 | 1.18E-53 | -1.69E-46 |
| MS.gene049285 | MS.gene89545 | 0.912474 | 1.87E-83 | -1.69E-46 |
| MS.gene049320 | MS.gene89545 | 0.899443 | 1.97E-77 | -1.69E-46 |
| MS.gene049427 | MS.gene89545 | 0.82395 | 1.05E-53 | -1.69E-46 |
| MS.gene049609 | MS.gene89545 | 0.854242 | 1.40E-61 | -1.69E-46 |
| MS.gene049614 | MS.gene89545 | 0.809409 | 1.92E-50 | -1.69E-46 |
| MS.gene049615 | MS.gene89545 | 0.800808 | 1.21E-48 | -1.69E-46 |
| MS.gene049633 | MS.gene89545 | 0.840059 | 1.09E-57 | -1.69E-46 |
| MS.gene049805 | MS.gene89545 | 0.831058 | 2.07E-55 | -1.69E-46 |
| MS.gene05010 | MS.gene89545 | 0.84333 | 1.50E-58 | -1.69E-46 |
| MS.gene050163 | MS.gene89545 | 0.895541 | 8.69E-76 | -1.69E-46 |
| MS.gene050165 | MS.gene89545 | 0.89756 | 1.25E-76 | -1.69E-46 |
| MS.gene050216 | MS.gene89545 | 0.816524 | 5.29E-52 | -1.69E-46 |
| MS.gene050323 | MS.gene89545 | 0.836225 | 1.06E-56 | -1.69E-46 |
| MS.gene050333 | MS.gene89545 | 0.912914 | 1.13E-83 | -1.69E-46 |
| MS.gene050444 | MS.gene89545 | 0.851496 | 8.55E-61 | -1.69E-46 |
| MS.gene050496 | MS.gene89545 | 0.803934 | 2.75E-49 | -1.69E-46 |
| MS.gene050504 | MS.gene89545 | 0.878825 | 2.05E-69 | -1.69E-46 |
| MS.gene050518 | MS.gene89545 | 0.877774 | 4.80E-69 | -1.69E-46 |
| MS.gene050538 | MS.gene89545 | 0.809401 | 1.93E-50 | -1.69E-46 |
| MS.gene050577 | MS.gene89545 | 0.804617 | 1.98E-49 | -1.69E-46 |
| MS.gene050593 | MS.gene89545 | 0.845017 | 5.28E-59 | -1.69E-46 |
| MS.gene050612 | MS.gene89545 | 0.902453 | 9.54E-79 | -1.69E-46 |
| MS.gene050662 | MS.gene89545 | 0.815542 | 8.77E-52 | -1.69E-46 |
| MS.gene050675 | MS.gene89545 | 0.868845 | 4.82E-66 | -1.69E-46 |
| MS.gene050699 | MS.gene89545 | 0.861227 | 1.19E-63 | -1.69E-46 |
| MS.gene050747 | MS.gene89545 | 0.813162 | 2.94E-51 | -1.69E-46 |
| MS.gene050767 | MS.gene89545 | 0.866508 | 2.71E-65 | -1.69E-46 |
| MS.gene050770 | MS.gene89545 | 0.869786 | 2.39E-66 | -1.69E-46 |
| MS.gene050772 | MS.gene89545 | 0.891702 | 3.12E-74 | -1.69E-46 |
| MS.gene05079 | MS.gene89545 | -0.823475 | 1.36E-53 | -1.69E-46 |
| MS.gene05080 | MS.gene89545 | 0.83305 | 6.66E-56 | -1.69E-46 |
| MS.gene050833 | MS.gene89545 | -0.801011 | 1.10E-48 | -1.69E-46 |
| MS.gene050868 | MS.gene89545 | 0.821685 | 3.54E-53 | -1.69E-46 |
| MS.gene050871 | MS.gene89545 | 0.818069 | 2.38E-52 | -1.69E-46 |
| MS.gene050876 | MS.gene89545 | 0.814455 | 1.53E-51 | -1.69E-46 |
| MS.gene050950 | MS.gene89545 | 0.814775 | 1.30E-51 | -1.69E-46 |
| MS.gene050986 | MS.gene89545 | 0.863319 | 2.71E-64 | -1.69E-46 |
| MS.gene05100 | MS.gene89545 | 0.860948 | 1.45E-63 | -1.69E-46 |
| MS.gene051228 | MS.gene89545 | 0.90632 | 1.68E-80 | -1.69E-46 |
| MS.gene051317 | MS.gene89545 | 0.831571 | 1.55E-55 | -1.69E-46 |
| MS.gene051434 | MS.gene89545 | 0.866524 | 2.68E-65 | -1.69E-46 |
| MS.gene051551 | MS.gene89545 | 0.841691 | 4.08E-58 | -1.69E-46 |
| MS.gene051617 | MS.gene89545 | 0.850058 | 2.17E-60 | -1.69E-46 |
| MS.gene051666 | MS.gene89545 | 0.844633 | 6.70E-59 | -1.69E-46 |
| MS.gene051694 | MS.gene89545 | 0.803287 | 3.75E-49 | -1.69E-46 |
| MS.gene051810 | MS.gene89545 | 0.899113 | 2.73E-77 | -1.69E-46 |
| MS.gene051858 | MS.gene89545 | 0.846532 | 2.05E-59 | -1.69E-46 |
| MS.gene051874 | MS.gene89545 | 0.80296 | 4.38E-49 | -1.69E-46 |
| MS.gene051949 | MS.gene89545 | 0.886373 | 3.62E-72 | -1.69E-46 |
| MS.gene052129 | MS.gene89545 | -0.800376 | 1.48E-48 | -1.69E-46 |
| MS.gene052164 | MS.gene89545 | 0.801102 | 1.06E-48 | -1.69E-46 |
| MS.gene052271 | MS.gene89545 | 0.888529 | 5.45E-73 | -1.69E-46 |
| MS.gene052290 | MS.gene89545 | 0.81604 | 6.79E-52 | -1.69E-46 |
| MS.gene052379 | MS.gene89545 | 0.877755 | 4.87E-69 | -1.69E-46 |
| MS.gene052484 | MS.gene89545 | 0.816774 | 4.65E-52 | -1.69E-46 |
| MS.gene052486 | MS.gene89545 | 0.804009 | 2.65E-49 | -1.69E-46 |
| MS.gene052506 | MS.gene89545 | 0.809129 | 2.20E-50 | -1.69E-46 |
| MS.gene052615 | MS.gene89545 | 0.807028 | 6.17E-50 | -1.69E-46 |
| MS.gene052822 | MS.gene89545 | 0.833688 | 4.62E-56 | -1.69E-46 |
| MS.gene052870 | MS.gene89545 | 0.872726 | 2.55E-67 | -1.69E-46 |
| MS.gene052872 | MS.gene89545 | 0.874557 | 6.15E-68 | -1.69E-46 |
| MS.gene052916 | MS.gene89545 | 0.864064 | 1.59E-64 | -1.69E-46 |
| MS.gene052966 | MS.gene89545 | 0.829759 | 4.30E-55 | -1.69E-46 |
| MS.gene053009 | MS.gene89545 | 0.842192 | 3.01E-58 | -1.69E-46 |
| MS.gene053103 | MS.gene89545 | 0.81877 | 1.65E-52 | -1.69E-46 |
| MS.gene053104 | MS.gene89545 | 0.886233 | 4.09E-72 | -1.69E-46 |
| MS.gene053107 | MS.gene89545 | 0.819545 | 1.10E-52 | -1.69E-46 |
| MS.gene053203 | MS.gene89545 | 0.812949 | 3.28E-51 | -1.69E-46 |
| MS.gene053207 | MS.gene89545 | 0.838456 | 2.85E-57 | -1.69E-46 |
| MS.gene053213 | MS.gene89545 | 0.87398 | 9.65E-68 | -1.69E-46 |
| MS.gene053284 | MS.gene89545 | 0.836002 | 1.21E-56 | -1.69E-46 |
| MS.gene053285 | MS.gene89545 | 0.830863 | 2.31E-55 | -1.69E-46 |
| MS.gene053286 | MS.gene89545 | 0.87188 | 4.88E-67 | -1.69E-46 |
| MS.gene053322 | MS.gene89545 | 0.870373 | 1.53E-66 | -1.69E-46 |
| MS.gene053384 | MS.gene89545 | 0.846309 | 2.35E-59 | -1.69E-46 |
| MS.gene053449 | MS.gene89545 | 0.806474 | 8.08E-50 | -1.69E-46 |
| MS.gene053479 | MS.gene89545 | 0.820691 | 6.00E-53 | -1.69E-46 |
| MS.gene053561 | MS.gene89545 | -0.829183 | 5.94E-55 | -1.69E-46 |
| MS.gene053574 | MS.gene89545 | 0.84325 | 1.57E-58 | -1.69E-46 |
| MS.gene053632 | MS.gene89545 | 0.835817 | 1.35E-56 | -1.69E-46 |
| MS.gene053824 | MS.gene89545 | 0.842867 | 1.99E-58 | -1.69E-46 |
| MS.gene053916 | MS.gene89545 | 0.858474 | 8.04E-63 | -1.69E-46 |
| MS.gene054374 | MS.gene89545 | 0.821599 | 3.71E-53 | -1.69E-46 |
| MS.gene054376 | MS.gene89545 | 0.842941 | 1.90E-58 | -1.69E-46 |
| MS.gene054543 | MS.gene89545 | 0.823167 | 1.60E-53 | -1.69E-46 |
| MS.gene054595 | MS.gene89545 | 0.815059 | 1.12E-51 | -1.69E-46 |
| MS.gene054600 | MS.gene89545 | 0.871098 | 8.85E-67 | -1.69E-46 |
| MS.gene054642 | MS.gene89545 | 0.921898 | 1.98E-88 | -1.69E-46 |
| MS.gene054802 | MS.gene89545 | 0.804579 | 2.02E-49 | -1.69E-46 |
| MS.gene054803 | MS.gene89545 | 0.803309 | 3.71E-49 | -1.69E-46 |
| MS.gene054804 | MS.gene89545 | 0.807545 | 4.80E-50 | -1.69E-46 |
| MS.gene054954 | MS.gene89545 | 0.889608 | 2.08E-73 | -1.69E-46 |
| MS.gene05505 | MS.gene89545 | 0.828837 | 7.20E-55 | -1.69E-46 |
| MS.gene055154 | MS.gene89545 | -0.820138 | 8.04E-53 | -1.69E-46 |
| MS.gene055206 | MS.gene89545 | 0.864632 | 1.06E-64 | -1.69E-46 |
| MS.gene055212 | MS.gene89545 | 0.868935 | 4.51E-66 | -1.69E-46 |
| MS.gene055224 | MS.gene89545 | 0.837708 | 4.43E-57 | -1.69E-46 |
| MS.gene055254 | MS.gene89545 | 0.809291 | 2.03E-50 | -1.69E-46 |
| MS.gene055289 | MS.gene89545 | 0.810741 | 9.91E-51 | -1.69E-46 |
| MS.gene055362 | MS.gene89545 | 0.889722 | 1.88E-73 | -1.69E-46 |
| MS.gene055397 | MS.gene89545 | 0.863118 | 3.13E-64 | -1.69E-46 |
| MS.gene055499 | MS.gene89545 | 0.844901 | 5.67E-59 | -1.69E-46 |
| MS.gene055502 | MS.gene89545 | 0.881921 | 1.61E-70 | -1.69E-46 |
| MS.gene055577 | MS.gene89545 | 0.866584 | 2.56E-65 | -1.69E-46 |
| MS.gene055634 | MS.gene89545 | 0.840952 | 6.38E-58 | -1.69E-46 |
| MS.gene055636 | MS.gene89545 | 0.897972 | 8.37E-77 | -1.69E-46 |
| MS.gene055638 | MS.gene89545 | 0.894707 | 1.91E-75 | -1.69E-46 |
| MS.gene055647 | MS.gene89545 | 0.860882 | 1.51E-63 | -1.69E-46 |
| MS.gene055722 | MS.gene89545 | 0.884006 | 2.77E-71 | -1.69E-46 |
| MS.gene055748 | MS.gene89545 | 0.867005 | 1.88E-65 | -1.69E-46 |
| MS.gene055750 | MS.gene89545 | 0.851657 | 7.69E-61 | -1.69E-46 |
| MS.gene055751 | MS.gene89545 | 0.873937 | 9.98E-68 | -1.69E-46 |
| MS.gene055776 | MS.gene89545 | 0.841161 | 5.62E-58 | -1.69E-46 |
| MS.gene05589 | MS.gene89545 | 0.806858 | 6.71E-50 | -1.69E-46 |
| MS.gene05592 | MS.gene89545 | 0.900169 | 9.58E-78 | -1.69E-46 |
| MS.gene055941 | MS.gene89545 | 0.852222 | 5.32E-61 | -1.69E-46 |
| MS.gene055942 | MS.gene89545 | -0.817242 | 3.65E-52 | -1.69E-46 |
| MS.gene055956 | MS.gene89545 | -0.871786 | 5.24E-67 | -1.69E-46 |
| MS.gene056083 | MS.gene89545 | 0.831018 | 2.12E-55 | -1.69E-46 |
| MS.gene056359 | MS.gene89545 | -0.801613 | 8.29E-49 | -1.69E-46 |
| MS.gene056374 | MS.gene89545 | 0.887885 | 9.63E-73 | -1.69E-46 |
| MS.gene056504 | MS.gene89545 | 0.846145 | 2.61E-59 | -1.69E-46 |
| MS.gene056510 | MS.gene89545 | 0.861459 | 1.01E-63 | -1.69E-46 |
| MS.gene056549 | MS.gene89545 | 0.847977 | 8.22E-60 | -1.69E-46 |
| MS.gene056657 | MS.gene89545 | 0.904477 | 1.18E-79 | -1.69E-46 |
| MS.gene056692 | MS.gene89545 | 0.853751 | 1.94E-61 | -1.69E-46 |
| MS.gene056856 | MS.gene89545 | -0.809735 | 1.63E-50 | -1.69E-46 |
| MS.gene056857 | MS.gene89545 | -0.805168 | 1.52E-49 | -1.69E-46 |
| MS.gene056897 | MS.gene89545 | 0.851073 | 1.13E-60 | -1.69E-46 |
| MS.gene056898 | MS.gene89545 | 0.839564 | 1.47E-57 | -1.69E-46 |
| MS.gene056947 | MS.gene89545 | 0.820307 | 7.35E-53 | -1.69E-46 |
| MS.gene057011 | MS.gene89545 | 0.814468 | 1.52E-51 | -1.69E-46 |
| MS.gene057076 | MS.gene89545 | 0.811834 | 5.74E-51 | -1.69E-46 |
| MS.gene057129 | MS.gene89545 | 0.887349 | 1.54E-72 | -1.69E-46 |
| MS.gene057246 | MS.gene89545 | 0.852892 | 3.43E-61 | -1.69E-46 |
| MS.gene057331 | MS.gene89545 | 0.895873 | 6.34E-76 | -1.69E-46 |
| MS.gene057416 | MS.gene89545 | 0.868629 | 5.66E-66 | -1.69E-46 |
| MS.gene057658 | MS.gene89545 | 0.817619 | 3.00E-52 | -1.69E-46 |
| MS.gene057831 | MS.gene89545 | 0.827498 | 1.51E-54 | -1.69E-46 |
| MS.gene057926 | MS.gene89545 | 0.862744 | 4.08E-64 | -1.69E-46 |
| MS.gene058019 | MS.gene89545 | 0.837995 | 3.74E-57 | -1.69E-46 |
| MS.gene058323 | MS.gene89545 | 0.846911 | 1.61E-59 | -1.69E-46 |
| MS.gene058338 | MS.gene89545 | 0.814602 | 1.42E-51 | -1.69E-46 |
| MS.gene05837 | MS.gene89545 | 0.826143 | 3.19E-54 | -1.69E-46 |
| MS.gene058419 | MS.gene89545 | 0.905821 | 2.86E-80 | -1.69E-46 |
| MS.gene058544 | MS.gene89545 | 0.843783 | 1.13E-58 | -1.69E-46 |
| MS.gene058723 | MS.gene89545 | 0.875259 | 3.55E-68 | -1.69E-46 |
| MS.gene058773 | MS.gene89545 | 0.898976 | 3.13E-77 | -1.69E-46 |
| MS.gene058838 | MS.gene89545 | 0.840685 | 7.49E-58 | -1.69E-46 |
| MS.gene058840 | MS.gene89545 | -0.807637 | 4.59E-50 | -1.69E-46 |
| MS.gene058978 | MS.gene89545 | -0.810544 | 1.09E-50 | -1.69E-46 |
| MS.gene059022 | MS.gene89545 | 0.893405 | 6.48E-75 | -1.69E-46 |
| MS.gene059023 | MS.gene89545 | 0.858834 | 6.27E-63 | -1.69E-46 |
| MS.gene059029 | MS.gene89545 | 0.895705 | 7.44E-76 | -1.69E-46 |
| MS.gene059079 | MS.gene89545 | 0.84147 | 4.66E-58 | -1.69E-46 |
| MS.gene05912 | MS.gene89545 | 0.809335 | 1.99E-50 | -1.69E-46 |
| MS.gene05913 | MS.gene89545 | 0.881219 | 2.88E-70 | -1.69E-46 |
| MS.gene059305 | MS.gene89545 | 0.806365 | 8.52E-50 | -1.69E-46 |
| MS.gene05934 | MS.gene89545 | 0.848248 | 6.92E-60 | -1.69E-46 |
| MS.gene059384 | MS.gene89545 | 0.818028 | 2.43E-52 | -1.69E-46 |
| MS.gene059536 | MS.gene89545 | 0.837038 | 6.58E-57 | -1.69E-46 |
| MS.gene059677 | MS.gene89545 | 0.899568 | 1.74E-77 | -1.69E-46 |
| MS.gene059718 | MS.gene89545 | 0.867 | 1.89E-65 | -1.69E-46 |
| MS.gene059756 | MS.gene89545 | 0.833754 | 4.45E-56 | -1.69E-46 |
| MS.gene059801 | MS.gene89545 | 0.805421 | 1.35E-49 | -1.69E-46 |
| MS.gene059858 | MS.gene89545 | 0.841429 | 4.78E-58 | -1.69E-46 |
| MS.gene059865 | MS.gene89545 | 0.86768 | 1.15E-65 | -1.69E-46 |
| MS.gene059902 | MS.gene89545 | 0.840525 | 8.25E-58 | -1.69E-46 |
| MS.gene059960 | MS.gene89545 | 0.883733 | 3.50E-71 | -1.69E-46 |
| MS.gene060193 | MS.gene89545 | 0.846404 | 2.22E-59 | -1.69E-46 |
| MS.gene060336 | MS.gene89545 | -0.815808 | 7.65E-52 | -1.69E-46 |
| MS.gene060337 | MS.gene89545 | -0.817283 | 3.57E-52 | -1.69E-46 |
| MS.gene060341 | MS.gene89545 | 0.819688 | 1.02E-52 | -1.69E-46 |
| MS.gene060482 | MS.gene89545 | 0.80058 | 1.35E-48 | -1.69E-46 |
| MS.gene060703 | MS.gene89545 | 0.869418 | 3.14E-66 | -1.69E-46 |
| MS.gene060727 | MS.gene89545 | 0.800111 | 1.68E-48 | -1.69E-46 |
| MS.gene060728 | MS.gene89545 | 0.830692 | 2.55E-55 | -1.69E-46 |
| MS.gene060762 | MS.gene89545 | 0.871329 | 7.43E-67 | -1.69E-46 |
| MS.gene06077 | MS.gene89545 | 0.839635 | 1.41E-57 | -1.69E-46 |
| MS.gene060804 | MS.gene89545 | 0.864481 | 1.18E-64 | -1.69E-46 |
| MS.gene060818 | MS.gene89545 | 0.854949 | 8.75E-62 | -1.69E-46 |
| MS.gene060835 | MS.gene89545 | 0.818395 | 2.00E-52 | -1.69E-46 |
| MS.gene06092 | MS.gene89545 | 0.852207 | 5.37E-61 | -1.69E-46 |
| MS.gene060982 | MS.gene89545 | 0.844313 | 8.17E-59 | -1.69E-46 |
| MS.gene061041 | MS.gene89545 | 0.832202 | 1.08E-55 | -1.69E-46 |
| MS.gene061134 | MS.gene89545 | 0.82926 | 5.69E-55 | -1.69E-46 |
| MS.gene061150 | MS.gene89545 | 0.890369 | 1.05E-73 | -1.69E-46 |
| MS.gene061200 | MS.gene89545 | 0.875722 | 2.46E-68 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene89545.t1 | MTR_1g069465 | 100.000 | 182 | 0 | 0 | 1 | 182 | 1 | 182 | 1.80e-136 | 378 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene89545.t1 | AT4G28660 | 68.966 | 145 | 45 | 0 | 37 | 181 | 37 | 181 | 1.03e-71 | 215 |
| MS.gene89545.t1 | AT4G28660 | 68.966 | 145 | 45 | 0 | 37 | 181 | 52 | 196 | 2.52e-71 | 214 |
Find 42 sgRNAs with CRISPR-Local
Find 88 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCCTTGAATGAATTGAATTT+TGG | 0.043355 | 1.1:+51186429 | None:intergenic |
| GGAGAAGTAGGCGACATTAC+TGG | 0.365446 | 1.1:-51186301 | MS.gene89545:CDS |
| CCCAAAATTCAATTCATTCA+AGG | 0.375868 | 1.1:-51186430 | MS.gene89545:CDS |
| AACTTAAATTCCTCATTCAA+TGG | 0.412356 | 1.1:-51186541 | MS.gene89545:CDS |
| TTCCCCGCCATCATGATAAC+CGG | 0.429760 | 1.1:+51186454 | None:intergenic |
| TGGAGTCCGCATTATATACT+TGG | 0.434350 | 1.1:+51186192 | None:intergenic |
| CAAATGGCATGGCTATCTTC+AGG | 0.434591 | 1.1:-51186354 | MS.gene89545:CDS |
| ATTTGCATGTGCCAAGTTTG+AGG | 0.436765 | 1.1:-51186513 | MS.gene89545:CDS |
| TAATGCGGACTCCACGGGAC+TGG | 0.442232 | 1.1:-51186183 | MS.gene89545:CDS |
| TTTGATTCTTCTGGAGAAGT+AGG | 0.453284 | 1.1:-51186313 | MS.gene89545:CDS |
| GGATAGGTTCATGAGATTCA+TGG | 0.460311 | 1.1:-51186161 | MS.gene89545:CDS |
| CATTTACATCAACAGATTGA+AGG | 0.465513 | 1.1:+51186251 | None:intergenic |
| GCCACTTCTTTAATTATCCA+TGG | 0.476745 | 1.1:-51186571 | MS.gene89545:CDS |
| GAGCGATACTCTGATGCTAA+TGG | 0.480616 | 1.1:-51186139 | MS.gene89545:CDS |
| AATTTAAGTTCAAACTTCCA+TGG | 0.492114 | 1.1:+51186554 | None:intergenic |
| AAATCGAAGGATGGAACAAA+TGG | 0.495690 | 1.1:-51186370 | MS.gene89545:CDS |
| CATGATAACCGGCATGTGCA+TGG | 0.501061 | 1.1:+51186465 | None:intergenic |
| ATGCCGGTTATCATGATGGC+GGG | 0.504435 | 1.1:-51186457 | MS.gene89545:CDS |
| CAGAAGCCACGCTCATACTC+AGG | 0.512959 | 1.1:-51187304 | MS.gene89545:intron |
| AATGCGGACTCCACGGGACT+GGG | 0.524053 | 1.1:-51186182 | MS.gene89545:CDS |
| GGACTCCACGGGACTGGGAT+AGG | 0.532501 | 1.1:-51186177 | MS.gene89545:CDS |
| GTATATAATGCGGACTCCAC+GGG | 0.541871 | 1.1:-51186188 | MS.gene89545:CDS |
| GCACATGCCGGTTATCATGA+TGG | 0.545209 | 1.1:-51186461 | MS.gene89545:CDS |
| ATACTTGGCTTCAATTCCTG+AGG | 0.546032 | 1.1:+51186207 | None:intergenic |
| ATCATCGGTAACCTCAAACT+TGG | 0.546137 | 1.1:+51186502 | None:intergenic |
| ATGATAACCGGCATGTGCAT+GGG | 0.547496 | 1.1:+51186466 | None:intergenic |
| AATTGAGTTGTAGAGTGGAG+AGG | 0.552148 | 1.1:+51187327 | None:intergenic |
| TCCATGGATAATTAAAGAAG+TGG | 0.555425 | 1.1:+51186570 | None:intergenic |
| TTTGTCAACGGAAAGCCCTC+AGG | 0.559746 | 1.1:-51186223 | MS.gene89545:CDS |
| AGTATATAATGCGGACTCCA+CGG | 0.565595 | 1.1:-51186189 | MS.gene89545:CDS |
| TACTTGGCTTCAATTCCTGA+GGG | 0.571164 | 1.1:+51186208 | None:intergenic |
| GTAAATGCTAAATTTGTCAA+CGG | 0.597700 | 1.1:-51186235 | MS.gene89545:CDS |
| AAGCTGACAAAATCGAAGGA+TGG | 0.601316 | 1.1:-51186379 | MS.gene89545:CDS |
| CAAATGTTGACCATTGAATG+AGG | 0.613868 | 1.1:+51186531 | None:intergenic |
| TGCCGGTTATCATGATGGCG+GGG | 0.623175 | 1.1:-51186456 | MS.gene89545:CDS |
| GAAGGATGGAACAAATGGCA+TGG | 0.640774 | 1.1:-51186365 | MS.gene89545:CDS |
| TGTAAAGCTGACAAAATCGA+AGG | 0.643773 | 1.1:-51186383 | MS.gene89545:CDS |
| CATGCCGGTTATCATGATGG+CGG | 0.658092 | 1.1:-51186458 | MS.gene89545:CDS |
| TTCTGAATTGAGTTGTAGAG+TGG | 0.666148 | 1.1:+51187322 | None:intergenic |
| ATGAACCTATCCCAGTCCCG+TGG | 0.667895 | 1.1:+51186172 | None:intergenic |
| TTTGTACCTGAGTATGAGCG+TGG | 0.677678 | 1.1:+51187298 | None:intergenic |
| TTGAAGCCAAGTATATAATG+CGG | 0.690031 | 1.1:-51186198 | MS.gene89545:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAATAAACAAAACAAAAGAT+GGG | - | chr1.1:51186420-51186439 | MS.gene89545:CDS | 15.0% |
| !! | CAAATAAACAAAACAAAAGA+TGG | - | chr1.1:51186419-51186438 | MS.gene89545:CDS | 20.0% |
| !! | GTAAATTCATAATATGAATC+AGG | - | chr1.1:51186319-51186338 | MS.gene89545:CDS | 20.0% |
| !! | TTAATTTACAAGTCAACAAT+TGG | + | chr1.1:51186382-51186401 | None:intergenic | 20.0% |
| !!! | CATCTTTTGTTTTGTTTATT+TGG | + | chr1.1:51186421-51186440 | None:intergenic | 20.0% |
| ! | AACTTAAATTCCTCATTCAA+TGG | - | chr1.1:51186931-51186950 | MS.gene89545:intron | 25.0% |
| ! | AAGTTCTCAAAACATTTCAT+AGG | + | chr1.1:51186246-51186265 | None:intergenic | 25.0% |
| ! | AATTTGCATTGTTCAGTATT+TGG | - | chr1.1:51186498-51186517 | MS.gene89545:CDS | 25.0% |
| ! | AGAGAATACTTGAAACTAAT+GGG | + | chr1.1:51186720-51186739 | None:intergenic | 25.0% |
| ! | ATCATATGTTCAAGATTTCA+AGG | + | chr1.1:51186792-51186811 | None:intergenic | 25.0% |
| ! | GTAAATGCTAAATTTGTCAA+CGG | - | chr1.1:51187237-51187256 | MS.gene89545:intron | 25.0% |
| ! | TAGAGAATACTTGAAACTAA+TGG | + | chr1.1:51186721-51186740 | None:intergenic | 25.0% |
| ! | TGTTGTCAAGAAAAAAATGT+TGG | + | chr1.1:51186476-51186495 | None:intergenic | 25.0% |
| ! | TTATACTTGTACTAGAATTG+TGG | - | chr1.1:51186613-51186632 | MS.gene89545:intron | 25.0% |
| !! | AATTTAAGTTCAAACTTCCA+TGG | + | chr1.1:51186921-51186940 | None:intergenic | 25.0% |
| !! | ATGAATCAGGTTAACATTTT+TGG | - | chr1.1:51186332-51186351 | MS.gene89545:CDS | 25.0% |
| !! | CCTTGAATGAATTGAATTTT+GGG | + | chr1.1:51187045-51187064 | None:intergenic | 25.0% |
| !! | TCCTTGAATGAATTGAATTT+TGG | + | chr1.1:51187046-51187065 | None:intergenic | 25.0% |
| !! | TGAATCAGGTTAACATTTTT+GGG | - | chr1.1:51186333-51186352 | MS.gene89545:CDS | 25.0% |
| !! | TGGTTTTTATATGATTGATG+AGG | - | chr1.1:51187191-51187210 | MS.gene89545:intron | 25.0% |
| !! | TTTTATATGATTGATGAGGA+AGG | - | chr1.1:51187195-51187214 | MS.gene89545:intron | 25.0% |
| !!! | ATGTTTTGAGAACTTATGTT+TGG | - | chr1.1:51186251-51186270 | MS.gene89545:CDS | 25.0% |
| !!! | CTTTGTTTGTTATTGTTTTG+TGG | - | chr1.1:51186218-51186237 | MS.gene89545:CDS | 25.0% |
| !!! | GCATCACTTTTAATTTTCTA+TGG | + | chr1.1:51186833-51186852 | None:intergenic | 25.0% |
| CAATTGGAGCAAACTATATA+AGG | + | chr1.1:51186366-51186385 | None:intergenic | 30.0% | |
| CATTTACATCAACAGATTGA+AGG | + | chr1.1:51187224-51187243 | None:intergenic | 30.0% | |
| CCCAAAATTCAATTCATTCA+AGG | - | chr1.1:51187042-51187061 | MS.gene89545:intron | 30.0% | |
| TCCATGGATAATTAAAGAAG+TGG | + | chr1.1:51186905-51186924 | None:intergenic | 30.0% | |
| TTGAAGCCAAGTATATAATG+CGG | - | chr1.1:51187274-51187293 | MS.gene89545:intron | 30.0% | |
| ! | GAATCAGGTTAACATTTTTG+GGG | - | chr1.1:51186334-51186353 | MS.gene89545:CDS | 30.0% |
| !!! | GGAGTTTTTTGAGTTATCAT+CGG | + | chr1.1:51186988-51187007 | None:intergenic | 30.0% |
| !!! | GTTTTGTTTATTTGGCATTC+TGG | + | chr1.1:51186413-51186432 | None:intergenic | 30.0% |
| AAATCGAAGGATGGAACAAA+TGG | - | chr1.1:51187102-51187121 | MS.gene89545:intron | 35.0% | |
| AGAACATCATACATACCATC+AGG | + | chr1.1:51186550-51186569 | None:intergenic | 35.0% | |
| ATGTTCAAGATTTCAAGGTC+AGG | + | chr1.1:51186787-51186806 | None:intergenic | 35.0% | |
| CAAATGTTGACCATTGAATG+AGG | + | chr1.1:51186944-51186963 | None:intergenic | 35.0% | |
| CCAGAAGAATCAAAAACTGA+GGG | + | chr1.1:51187153-51187172 | None:intergenic | 35.0% | |
| GAACATCATACATACCATCA+GGG | + | chr1.1:51186549-51186568 | None:intergenic | 35.0% | |
| GATGGTATGTATGATGTTCT+TGG | - | chr1.1:51186550-51186569 | MS.gene89545:CDS | 35.0% | |
| GCCACTTCTTTAATTATCCA+TGG | - | chr1.1:51186901-51186920 | MS.gene89545:intron | 35.0% | |
| TACTAGAATTGTGGAGTAAC+AGG | - | chr1.1:51186622-51186641 | MS.gene89545:intron | 35.0% | |
| TCCAGAAGAATCAAAAACTG+AGG | + | chr1.1:51187154-51187173 | None:intergenic | 35.0% | |
| TGTAAAGCTGACAAAATCGA+AGG | - | chr1.1:51187089-51187108 | MS.gene89545:intron | 35.0% | |
| TGTTCAAGATTTCAAGGTCA+GGG | + | chr1.1:51186786-51186805 | None:intergenic | 35.0% | |
| TGTTTATTTGGCATTCTGGA+AGG | + | chr1.1:51186409-51186428 | None:intergenic | 35.0% | |
| ! | GATTTTGTCAGCTTTACATC+AGG | + | chr1.1:51187087-51187106 | None:intergenic | 35.0% |
| ! | TTCTGAATTGAGTTGTAGAG+TGG | + | chr1.1:51186153-51186172 | None:intergenic | 35.0% |
| ! | TTTGATTCTTCTGGAGAAGT+AGG | - | chr1.1:51187159-51187178 | MS.gene89545:intron | 35.0% |
| !!! | GGTTAACATTTTTGGGGTTA+AGG | - | chr1.1:51186340-51186359 | MS.gene89545:CDS | 35.0% |
| AAGCTGACAAAATCGAAGGA+TGG | - | chr1.1:51187093-51187112 | MS.gene89545:intron | 40.0% | |
| AGTATATAATGCGGACTCCA+CGG | - | chr1.1:51187283-51187302 | MS.gene89545:intron | 40.0% | |
| ATACTTGGCTTCAATTCCTG+AGG | + | chr1.1:51187268-51187287 | None:intergenic | 40.0% | |
| ATCATCGGTAACCTCAAACT+TGG | + | chr1.1:51186973-51186992 | None:intergenic | 40.0% | |
| ATGTTCTTGGCAACACTCTA+TGG | - | chr1.1:51186563-51186582 | MS.gene89545:CDS | 40.0% | |
| ATTTGCATGTGCCAAGTTTG+AGG | - | chr1.1:51186959-51186978 | MS.gene89545:intron | 40.0% | |
| CAACACTCTATGGAACAGAA+TGG | - | chr1.1:51186573-51186592 | MS.gene89545:CDS | 40.0% | |
| CTATGGAACAGAATGGCATT+GGG | - | chr1.1:51186580-51186599 | MS.gene89545:intron | 40.0% | |
| GGATAGGTTCATGAGATTCA+TGG | - | chr1.1:51187311-51187330 | MS.gene89545:CDS | 40.0% | |
| GTTCAAGATTTCAAGGTCAG+GGG | + | chr1.1:51186785-51186804 | None:intergenic | 40.0% | |
| TAATATAGCTTCCACCCTGA+TGG | - | chr1.1:51186532-51186551 | MS.gene89545:CDS | 40.0% | |
| TACTTGGCTTCAATTCCTGA+GGG | + | chr1.1:51187267-51187286 | None:intergenic | 40.0% | |
| TCTATGGAACAGAATGGCAT+TGG | - | chr1.1:51186579-51186598 | MS.gene89545:intron | 40.0% | |
| TGGAGTCCGCATTATATACT+TGG | + | chr1.1:51187283-51187302 | None:intergenic | 40.0% | |
| ! | AATTGAGTTGTAGAGTGGAG+AGG | + | chr1.1:51186148-51186167 | None:intergenic | 40.0% |
| !! | CCCTCAGTTTTTGATTCTTC+TGG | - | chr1.1:51187150-51187169 | MS.gene89545:intron | 40.0% |
| AAAAACTCCCATGCACATGC+CGG | - | chr1.1:51186999-51187018 | MS.gene89545:intron | 45.0% | |
| ATGATAACCGGCATGTGCAT+GGG | + | chr1.1:51187009-51187028 | None:intergenic | 45.0% | |
| ATTTCAAGGTCAGGGGTCAA+GGG | + | chr1.1:51186778-51186797 | None:intergenic | 45.0% | |
| CAAATGGCATGGCTATCTTC+AGG | - | chr1.1:51187118-51187137 | MS.gene89545:intron | 45.0% | |
| CATCATACATACCATCAGGG+TGG | + | chr1.1:51186546-51186565 | None:intergenic | 45.0% | |
| GAAGGATGGAACAAATGGCA+TGG | - | chr1.1:51187107-51187126 | MS.gene89545:intron | 45.0% | |
| GAGCGATACTCTGATGCTAA+TGG | - | chr1.1:51187333-51187352 | MS.gene89545:CDS | 45.0% | |
| GTATATAATGCGGACTCCAC+GGG | - | chr1.1:51187284-51187303 | MS.gene89545:intron | 45.0% | |
| TTTGTACCTGAGTATGAGCG+TGG | + | chr1.1:51186177-51186196 | None:intergenic | 45.0% | |
| CATGATAACCGGCATGTGCA+TGG | + | chr1.1:51187010-51187029 | None:intergenic | 50.0% | |
| GATTTCAAGGTCAGGGGTCA+AGG | + | chr1.1:51186779-51186798 | None:intergenic | 50.0% | |
| GGAGAAGTAGGCGACATTAC+TGG | - | chr1.1:51187171-51187190 | MS.gene89545:intron | 50.0% | |
| TTCCCCGCCATCATGATAAC+CGG | + | chr1.1:51187021-51187040 | None:intergenic | 50.0% | |
| TTTGTCAACGGAAAGCCCTC+AGG | - | chr1.1:51187249-51187268 | MS.gene89545:intron | 50.0% | |
| ! | ATGCCGGTTATCATGATGGC+GGG | - | chr1.1:51187015-51187034 | MS.gene89545:intron | 50.0% |
| ! | CATGCCGGTTATCATGATGG+CGG | - | chr1.1:51187014-51187033 | MS.gene89545:intron | 50.0% |
| ! | GCACATGCCGGTTATCATGA+TGG | - | chr1.1:51187011-51187030 | MS.gene89545:intron | 50.0% |
| ATGAACCTATCCCAGTCCCG+TGG | + | chr1.1:51187303-51187322 | None:intergenic | 55.0% | |
| CAGAAGCCACGCTCATACTC+AGG | - | chr1.1:51186168-51186187 | MS.gene89545:CDS | 55.0% | |
| ! | TGCCGGTTATCATGATGGCG+GGG | - | chr1.1:51187016-51187035 | MS.gene89545:intron | 55.0% |
| AATGCGGACTCCACGGGACT+GGG | - | chr1.1:51187290-51187309 | MS.gene89545:intron | 60.0% | |
| TAATGCGGACTCCACGGGAC+TGG | - | chr1.1:51187289-51187308 | MS.gene89545:intron | 60.0% | |
| GGACTCCACGGGACTGGGAT+AGG | - | chr1.1:51187295-51187314 | MS.gene89545:intron | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.1 | gene | 51186117 | 51187377 | 51186117 | ID=MS.gene89545 |
| chr1.1 | mRNA | 51186117 | 51187377 | 51186117 | ID=MS.gene89545.t1;Parent=MS.gene89545 |
| chr1.1 | exon | 51187305 | 51187377 | 51187305 | ID=MS.gene89545.t1.exon1;Parent=MS.gene89545.t1 |
| chr1.1 | CDS | 51187305 | 51187377 | 51187305 | ID=cds.MS.gene89545.t1;Parent=MS.gene89545.t1 |
| chr1.1 | exon | 51186117 | 51186592 | 51186117 | ID=MS.gene89545.t1.exon2;Parent=MS.gene89545.t1 |
| chr1.1 | CDS | 51186117 | 51186592 | 51186117 | ID=cds.MS.gene89545.t1;Parent=MS.gene89545.t1 |
| Gene Sequence |
| Protein sequence |
