AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene76628


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene76628.t1 MTR_5g041910 95.312 64 3 0 27 90 198 261 1.57e-37 126
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene76628.t1 AT1G14150 56.667 90 30 2 1 90 110 190 2.42e-27 98.6

Find 12 sgRNAs with CRISPR-Local

Find 105 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCAATGTCTTCTCGTAGTAT+AGG 0.434954 5.1:+31556512 None:intergenic
ATTCATATACCTTTGGTGTC+CGG 0.448494 5.1:+31556539 None:intergenic
TACCAGCATAGCAGGTCAGC+AGG 0.454532 5.1:-31558422 MS.gene76628:intron
AGGTATGATTCATATACCTT+TGG 0.468647 5.1:+31556532 None:intergenic
TGTGAGTATACCAGCATAGC+AGG 0.495736 5.1:-31558430 MS.gene76628:CDS
TACCTGCTGACCTGCTATGC+TGG 0.514831 5.1:+31558420 None:intergenic
TTCAGATGGATCGTTATGTC+CGG 0.516551 5.1:-31556558 MS.gene76628:intron
GAAATCAATAGATGAACTTG+TGG 0.590899 5.1:-31556491 MS.gene76628:CDS
TGTTAAAACTGCTAATGAGT+TGG 0.596114 5.1:-31557445 MS.gene76628:CDS
TCGTTATGTCCGGACACCAA+AGG 0.636412 5.1:-31556548 MS.gene76628:CDS
TTTGCCTGAAAATCGTCGCA+AGG 0.639622 5.1:-31557472 MS.gene76628:CDS
GTTGGTTGATAACTTGACTG+AGG 0.748637 5.1:-31557427 MS.gene76628:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GAAAAAAATGTAAATATTTA+TGG + chr5.1:31556823-31556842 None:intergenic 10.0%
!! TGAAATTGTTAATGAATATA+AGG - chr5.1:31558198-31558217 MS.gene76628:intron 15.0%
!! TTAAATGTAATTATATGTGT+CGG - chr5.1:31558100-31558119 MS.gene76628:intron 15.0%
!! TTGAAGTTGAATAAAAAAAA+TGG - chr5.1:31556504-31556523 MS.gene76628:CDS 15.0%
!!! AAAATTAAGCATTTTCTTTT+TGG + chr5.1:31556847-31556866 None:intergenic 15.0%
!!! TCTATAGTTGAATTTTTTTA+AGG + chr5.1:31557824-31557843 None:intergenic 15.0%
!!! TTTTAAAAAAATTGATGATG+TGG - chr5.1:31556569-31556588 MS.gene76628:intron 15.0%
!! AATGTAAATATTTATGGTAG+TGG + chr5.1:31556817-31556836 None:intergenic 20.0%
!! ATAAGATACATAATCTTTCA+AGG + chr5.1:31558357-31558376 None:intergenic 20.0%
!! ATGTAAATATTTATGGTAGT+GGG + chr5.1:31556816-31556835 None:intergenic 20.0%
!! TAAGATACATAATCTTTCAA+GGG + chr5.1:31558356-31558375 None:intergenic 20.0%
!! TCATAAGATAATTTAGTCAT+TGG - chr5.1:31557672-31557691 MS.gene76628:intron 20.0%
!!! ATTTTTTGTGTGGATATTTT+TGG + chr5.1:31557059-31557078 None:intergenic 20.0%
!!! TGCATTTTTAGTATATGATT+AGG - chr5.1:31558254-31558273 MS.gene76628:intron 20.0%
! AAATGTTGGAAACTTTATCT+TGG - chr5.1:31557395-31557414 MS.gene76628:intron 25.0%
! AAGATACATAATCTTTCAAG+GGG + chr5.1:31558355-31558374 None:intergenic 25.0%
! AATATAAGGTACAATACAAG+AGG - chr5.1:31558212-31558231 MS.gene76628:intron 25.0%
! AATCTATGAAGTATGAATAC+AGG - chr5.1:31557982-31558001 MS.gene76628:intron 25.0%
! ATGAAGATTTAGTTATGGTA+TGG + chr5.1:31557033-31557052 None:intergenic 25.0%
! TGTGTTTAATTAGTTAAGCT+GGG - chr5.1:31557795-31557814 MS.gene76628:intron 25.0%
! TTGTGTTTAATTAGTTAAGC+TGG - chr5.1:31557794-31557813 MS.gene76628:intron 25.0%
!! ATGATTTTGAATGGTGTATT+TGG + chr5.1:31557009-31557028 None:intergenic 25.0%
!! ATTTGGACTATGGAAATTTT+TGG + chr5.1:31556992-31557011 None:intergenic 25.0%
!! TTTGGACTATGGAAATTTTT+GGG + chr5.1:31556991-31557010 None:intergenic 25.0%
!!! AATTTATATGCCTGTTTTTG+AGG + chr5.1:31557919-31557938 None:intergenic 25.0%
AAGACCCAATTGTATAAACA+GGG - chr5.1:31556939-31556958 MS.gene76628:intron 30.0%
ACATAATCTTAGGGAAATGT+TGG - chr5.1:31557381-31557400 MS.gene76628:intron 30.0%
AGACTCAAATTGTCAATTCA+TGG - chr5.1:31557841-31557860 MS.gene76628:intron 30.0%
AGGTATGATTCATATACCTT+TGG + chr5.1:31558432-31558451 None:intergenic 30.0%
AGTATATGATTAGGTTCGAT+TGG - chr5.1:31558263-31558282 MS.gene76628:intron 30.0%
ATTTAGCTATCCTCAAAAAC+AGG - chr5.1:31557906-31557925 MS.gene76628:intron 30.0%
GAAACAGAATTATCTTGAAC+AGG - chr5.1:31557444-31557463 MS.gene76628:CDS 30.0%
GAAATCAATAGATGAACTTG+TGG - chr5.1:31558470-31558489 MS.gene76628:CDS 30.0%
GGTGTATGAAGATTTAGTTA+TGG + chr5.1:31557038-31557057 None:intergenic 30.0%
TGAAGGGTTACATAATCTTA+GGG - chr5.1:31557372-31557391 MS.gene76628:intron 30.0%
TGTTAAAACTGCTAATGAGT+TGG - chr5.1:31557516-31557535 MS.gene76628:intron 30.0%
TTAAGCACTCTCTCAATTAT+TGG + chr5.1:31556596-31556615 None:intergenic 30.0%
TTGAACCAGATGAACTAATT+TGG + chr5.1:31557746-31557765 None:intergenic 30.0%
! TGGTATGGTATGATTTTGAA+TGG + chr5.1:31557018-31557037 None:intergenic 30.0%
!! AACATTTCTAGCAACATCAA+TGG - chr5.1:31557216-31557235 MS.gene76628:intron 30.0%
!! ATTTTTGGGCAGGTTTTTAT+TGG + chr5.1:31556977-31556996 None:intergenic 30.0%
!! GTTTGAAGTGTTTTTGTGTT+TGG + chr5.1:31557131-31557150 None:intergenic 30.0%
!!! AAGGAACATTTTTTTATGCC+AGG - chr5.1:31557307-31557326 MS.gene76628:intron 30.0%
!!! AGGAACATTTTTTTATGCCA+GGG - chr5.1:31557308-31557327 MS.gene76628:intron 30.0%
ACTAGCACTAGCACAAAATT+GGG - chr5.1:31557267-31557286 MS.gene76628:intron 35.0%
AGGATAAAACAAACAGCTGA+AGG - chr5.1:31557355-31557374 MS.gene76628:intron 35.0%
ATTGTATAAACAGGGCAACA+CGG - chr5.1:31556947-31556966 MS.gene76628:intron 35.0%
CAAGACCCAATTGTATAAAC+AGG - chr5.1:31556938-31556957 MS.gene76628:intron 35.0%
CCAACAATGAATGAATGACT+TGG - chr5.1:31556867-31556886 MS.gene76628:intron 35.0%
CCAAGTCATTCATTCATTGT+TGG + chr5.1:31556870-31556889 None:intergenic 35.0%
CTGAAGGGTTACATAATCTT+AGG - chr5.1:31557371-31557390 MS.gene76628:intron 35.0%
GAAAATTGCATTTAGAGACG+AGG - chr5.1:31556710-31556729 MS.gene76628:intron 35.0%
GGATAAAACAAACAGCTGAA+GGG - chr5.1:31557356-31557375 MS.gene76628:intron 35.0%
GGTTCGATTGGCAAAATAAA+AGG - chr5.1:31558275-31558294 MS.gene76628:intron 35.0%
GTTGCCCTGTTTATACAATT+GGG + chr5.1:31556946-31556965 None:intergenic 35.0%
TCAATGTCTTCTCGTAGTAT+AGG + chr5.1:31558452-31558471 None:intergenic 35.0%
TGAATGGTGTATTTGGACTA+TGG + chr5.1:31557002-31557021 None:intergenic 35.0%
TGTTGCCCTGTTTATACAAT+TGG + chr5.1:31556947-31556966 None:intergenic 35.0%
TGTTGGAAACTTTATCTTGG+AGG - chr5.1:31557398-31557417 MS.gene76628:intron 35.0%
TTCATGCATTGTTGTGATGA+TGG - chr5.1:31556748-31556767 MS.gene76628:intron 35.0%
TTCGTCCAAATTAGTTCATC+TGG - chr5.1:31557738-31557757 MS.gene76628:intron 35.0%
! TGGAGTTTTCTCAACTCAAA+TGG - chr5.1:31556887-31556906 MS.gene76628:intron 35.0%
! TTTCTAGCAACATCAATGGT+TGG - chr5.1:31557220-31557239 MS.gene76628:intron 35.0%
!! ATTCATATACCTTTGGTGTC+CGG + chr5.1:31558425-31558444 None:intergenic 35.0%
!!! GTTTTTGTGTTTGGTGATGT+GGG + chr5.1:31557122-31557141 None:intergenic 35.0%
!!! GTTTTTTTTCCCTCTTCAGA+TGG - chr5.1:31558389-31558408 MS.gene76628:intron 35.0%
!!! TGGACATGGTATTTTTTGTG+TGG + chr5.1:31557069-31557088 None:intergenic 35.0%
!!! TGTTTTTGTGTTTGGTGATG+TGG + chr5.1:31557123-31557142 None:intergenic 35.0%
ACATAACGATCCATCTGAAG+AGG + chr5.1:31558402-31558421 None:intergenic 40.0%
AGGCAGAGACATATGAAGTA+TGG - chr5.1:31558002-31558021 MS.gene76628:intron 40.0%
CACTAGCACTAGCACAAAAT+TGG - chr5.1:31557266-31557285 MS.gene76628:intron 40.0%
CATAACGATCCATCTGAAGA+GGG + chr5.1:31558401-31558420 None:intergenic 40.0%
CTAGCACTAGCACAAAATTG+GGG - chr5.1:31557268-31557287 MS.gene76628:intron 40.0%
TGGATAACAAAAGTTCCACG+TGG - chr5.1:31556907-31556926 MS.gene76628:intron 40.0%
TTCAGATGGATCGTTATGTC+CGG - chr5.1:31558403-31558422 MS.gene76628:intron 40.0%
! AGTGGGTTTGATGCATTTGT+AGG + chr5.1:31556799-31556818 None:intergenic 40.0%
! CAATTTTGTGCTAGTGCTAG+TGG + chr5.1:31557268-31557287 None:intergenic 40.0%
!! GACTATGGAAATTTTTGGGC+AGG + chr5.1:31556987-31557006 None:intergenic 40.0%
!! GTTGGTTGATAACTTGACTG+AGG - chr5.1:31557534-31557553 MS.gene76628:intron 40.0%
ATCCTTAACACTGCATCCTC+AGG + chr5.1:31557340-31557359 None:intergenic 45.0%
ATGGAGTAGTGTAGTGGCAT+GGG + chr5.1:31557098-31557117 None:intergenic 45.0%
TCCTTAACACTGCATCCTCA+GGG + chr5.1:31557339-31557358 None:intergenic 45.0%
TGTGAGTATACCAGCATAGC+AGG - chr5.1:31556531-31556550 MS.gene76628:CDS 45.0%
TTTGCCTGAAAATCGTCGCA+AGG - chr5.1:31557489-31557508 MS.gene76628:CDS 45.0%
! GGGCACACGTTCTTTTATGA+AGG - chr5.1:31557288-31557307 MS.gene76628:intron 45.0%
! TACTCCTTGCGACGATTTTC+AGG + chr5.1:31557496-31557515 None:intergenic 45.0%
!!! ATTTTTATAAAGAAAAAAAT+TGG + chr5.1:31556690-31556709 None:intergenic 5.0%
CATGGAGTAGTGTAGTGGCA+TGG + chr5.1:31557099-31557118 None:intergenic 50.0%
CCTTAACACTGCATCCTCAG+GGG + chr5.1:31557338-31557357 None:intergenic 50.0%
GCACAAACGCTCCTCGAATT+AGG + chr5.1:31558149-31558168 None:intergenic 50.0%
GGTCTTGAGATTATGCCACG+TGG + chr5.1:31556925-31556944 None:intergenic 50.0%
TCGTTATGTCCGGACACCAA+AGG - chr5.1:31558413-31558432 MS.gene76628:intron 50.0%
!! GAGGAGCGTTTGTGCTTCAT+AGG - chr5.1:31558154-31558173 MS.gene76628:intron 50.0%
!! TGTGTTTGGTGATGTGGGCA+TGG + chr5.1:31557117-31557136 None:intergenic 50.0%
ACCAGGACACGCCTAATTCG+AGG - chr5.1:31558135-31558154 MS.gene76628:intron 55.0%
CCCCTGAGGATGCAGTGTTA+AGG - chr5.1:31557335-31557354 MS.gene76628:intron 55.0%
GTGGGCATGGAGTAGTGTAG+TGG + chr5.1:31557104-31557123 None:intergenic 55.0%
TACCAGCATAGCAGGTCAGC+AGG - chr5.1:31556539-31556558 MS.gene76628:CDS 55.0%
TACCTGCTGACCTGCTATGC+TGG + chr5.1:31556544-31556563 None:intergenic 55.0%
TCCTCGAATTAGGCGTGTCC+TGG + chr5.1:31558139-31558158 None:intergenic 55.0%
TGTAGTGGCATGGGTGAAGC+TGG + chr5.1:31557089-31557108 None:intergenic 55.0%
!! GTCGGTGTTGTATCGACACC+AGG - chr5.1:31558118-31558137 MS.gene76628:intron 55.0%
GGCATGGGTGAAGCTGGACA+TGG + chr5.1:31557083-31557102 None:intergenic 60.0%
TATGCCAGGGCTATCCCCTG+AGG - chr5.1:31557321-31557340 MS.gene76628:intron 60.0%
GCATCCTCAGGGGATAGCCC+TGG + chr5.1:31557328-31557347 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr5.1 gene 31556477 31558506 31556477 ID=MS.gene76628
chr5.1 mRNA 31556477 31558506 31556477 ID=MS.gene76628.t1;Parent=MS.gene76628
chr5.1 exon 31558423 31558506 31558423 ID=MS.gene76628.t1.exon1;Parent=MS.gene76628.t1
chr5.1 CDS 31558423 31558506 31558423 ID=cds.MS.gene76628.t1;Parent=MS.gene76628.t1
chr5.1 exon 31557428 31557517 31557428 ID=MS.gene76628.t1.exon2;Parent=MS.gene76628.t1
chr5.1 CDS 31557428 31557517 31557428 ID=cds.MS.gene76628.t1;Parent=MS.gene76628.t1
chr5.1 exon 31556477 31556575 31556477 ID=MS.gene76628.t1.exon3;Parent=MS.gene76628.t1
chr5.1 CDS 31556477 31556575 31556477 ID=cds.MS.gene76628.t1;Parent=MS.gene76628.t1
Gene Sequence

>MS.gene76628

ATGGAGTTACGCGTTATTTTGTTGTTTTTGAAGTTGAATAAAAAAAATGGTAAATGTGAGTATACCAGCATAGCAGGTCAGCAGGTAATTTGTTTTAAAAAAATTGATGATGTGGACCAATAATTGAGAGAGTGCTTAAGAGAGAGTGTAAAAGCAACTGCAGCTAGCATTTCTCTTTAGATACATATAATTGACATTTTAACTACGTTACCAATTTTTTTCTTTATAAAAATGAAAATTGCATTTAGAGACGAGGTATTTAAAAAAGATATTCATGCATTGTTGTGATGATGGTTAATAATAGTGAAAATTCTATGAACCTACAAATGCATCAAACCCACTACCATAAATATTTACATTTTTTTCACCAAAAAGAAAATGCTTAATTTTCCAACAATGAATGAATGACTTGGAGTTTTCTCAACTCAAATGGATAACAAAAGTTCCACGTGGCATAATCTCAAGACCCAATTGTATAAACAGGGCAACACGGTCAGCCAATAAAAACCTGCCCAAAAATTTCCATAGTCCAAATACACCATTCAAAATCATACCATACCATAACTAAATCTTCATACACCAAAAATATCCACACAAAAAATACCATGTCCAGCTTCACCCATGCCACTACACTACTCCATGCCCACATCACCAAACACAAAAACACTTCAAACAACAACATTAACAACAACTCTTTATCTTCTAAACAAAATGTGTCTAATAGAAGAAATTTAGTTTCAACATTTCTAGCAACATCAATGGTTGGAGTAGTACATGTTGCAACAGCACCACTAGCACTAGCACAAAATTGGGGCACACGTTCTTTTATGAAGGAACATTTTTTTATGCCAGGGCTATCCCCTGAGGATGCAGTGTTAAGGATAAAACAAACAGCTGAAGGGTTACATAATCTTAGGGAAATGTTGGAAACTTTATCTTGGAGGTATGTTATGTTTTATATAAGATTGAAACAGAATTATCTTGAACAGGATTTGAAAAATGCTATCACTACTTTGCCTGAAAATCGTCGCAAGGAGTATGTTAAAACTGCTAATGAGTTGGTTGATAACTTGACTGAGGTATGTATGATTAATTTTTAGTACTTGTTTTGTCATTTTGATTAATGAATTGAATTAGTCTATGAATAGATATACAAATTCTATAATATTGATATATGTTTTTTTTAGAATTATAGTCATAAGATAATTTAGTCATTGGAATTAATGAATTTTGAAAATAATTTTGAAGATTAAAATTTCATTTCGTCCAAATTAGTTCATCTGGTTCAAATTGTCAGTTTTATAATTTATTTTGAATTTGTGTTTAATTAGTTAAGCTGGGTTACCTTAAAAAAATTCAACTATAGACTCAAATTGTCAATTCATGGATTAATTTGAGTGATGAAACATGAGTTTAACATGTTTGTATAATTTAGCTATCCTCAAAAACAGGCATATAAATTAGTTTTACACTATCATTCAATCTGAACTCTATTTTATTATGTTAATCTATGAAGTATGAATACAGGCAGAGACATATGAAGTATGGATACAGACACAGACATCAGACAAGACATGAACACTAACATGTCGATATCGCTAATAATTTGATAAAATAATATAATTAAATGTAATTATATGTGTCGGTGTTGTATCGACACCAGGACACGCCTAATTCGAGGAGCGTTTGTGCTTCATAGGTTTTAATTATATCGTTATAGATGAAATTGTTAATGAATATAAGGTACAATACAAGAGGCATTGTAGTGATCATTATCTGCATTTTTAGTATATGATTAGGTTCGATTGGCAAAATAAAAGGACTTAATATCTAATTATCTATAGCTTAACAATAGAAGTTAATTATTACTAGTCACCCCTTGAAAGATTATGTATCTTATACTGTGATTTATGTTTTTTTTCCCTCTTCAGATGGATCGTTATGTCCGGACACCAAAGGTATATGAATCATACCTATACTACGAGAAGACATTGAAATCAATAGATGAACTTGTGGCTCTGTTAGCATAG

Protein sequence

>MS.gene76628.t1

MELRVILLFLKLNKKNGKCEYTSIAGQQDLKNAITTLPENRRKEYVKTANELVDNLTEMDRYVRTPKVYESYLYYEKTLKSIDELVALLA