Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene31724.t1 | XP_013466249.1 | 96.9 | 130 | 4 | 0 | 1 | 130 | 1 | 130 | 4.40E-61 | 243.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene31724.t1 | Q9ZT05 | 96.9 | 127 | 4 | 0 | 1 | 127 | 1 | 127 | 6.8e-63 | 241.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene31724.t1 | I3SQF1 | 96.9 | 130 | 4 | 0 | 1 | 130 | 1 | 130 | 3.1e-61 | 243.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049217 | MS.gene31724 | 0.817443 | 3.29E-52 | -1.69E-46 |
| MS.gene049285 | MS.gene31724 | 0.821729 | 3.46E-53 | -1.69E-46 |
| MS.gene049320 | MS.gene31724 | 0.841895 | 3.60E-58 | -1.69E-46 |
| MS.gene049351 | MS.gene31724 | 0.810443 | 1.15E-50 | -1.69E-46 |
| MS.gene049356 | MS.gene31724 | 0.817901 | 2.59E-52 | -1.69E-46 |
| MS.gene049369 | MS.gene31724 | 0.841072 | 5.93E-58 | -1.69E-46 |
| MS.gene049427 | MS.gene31724 | 0.82665 | 2.41E-54 | -1.69E-46 |
| MS.gene049609 | MS.gene31724 | 0.839463 | 1.56E-57 | -1.69E-46 |
| MS.gene049614 | MS.gene31724 | 0.832683 | 8.22E-56 | -1.69E-46 |
| MS.gene049661 | MS.gene31724 | 0.8484 | 6.28E-60 | -1.69E-46 |
| MS.gene049662 | MS.gene31724 | 0.834091 | 3.66E-56 | -1.69E-46 |
| MS.gene049760 | MS.gene31724 | 0.80366 | 3.14E-49 | -1.69E-46 |
| MS.gene049805 | MS.gene31724 | 0.866128 | 3.57E-65 | -1.69E-46 |
| MS.gene050076 | MS.gene31724 | 0.857377 | 1.70E-62 | -1.69E-46 |
| MS.gene050163 | MS.gene31724 | 0.816583 | 5.13E-52 | -1.69E-46 |
| MS.gene050333 | MS.gene31724 | 0.812411 | 4.30E-51 | -1.69E-46 |
| MS.gene050397 | MS.gene31724 | 0.821369 | 4.19E-53 | -1.69E-46 |
| MS.gene050432 | MS.gene31724 | 0.902799 | 6.70E-79 | -1.69E-46 |
| MS.gene050520 | MS.gene31724 | 0.815225 | 1.03E-51 | -1.69E-46 |
| MS.gene050593 | MS.gene31724 | 0.824844 | 6.48E-54 | -1.69E-46 |
| MS.gene050612 | MS.gene31724 | 0.808308 | 3.30E-50 | -1.69E-46 |
| MS.gene050686 | MS.gene31724 | 0.830969 | 2.18E-55 | -1.69E-46 |
| MS.gene050699 | MS.gene31724 | 0.808939 | 2.42E-50 | -1.69E-46 |
| MS.gene050767 | MS.gene31724 | 0.896228 | 4.51E-76 | -1.69E-46 |
| MS.gene050770 | MS.gene31724 | 0.89798 | 8.30E-77 | -1.69E-46 |
| MS.gene050772 | MS.gene31724 | 0.86633 | 3.09E-65 | -1.69E-46 |
| MS.gene05101 | MS.gene31724 | 0.833675 | 4.65E-56 | -1.69E-46 |
| MS.gene05105 | MS.gene31724 | 0.851761 | 7.19E-61 | -1.69E-46 |
| MS.gene051228 | MS.gene31724 | 0.874737 | 5.34E-68 | -1.69E-46 |
| MS.gene051387 | MS.gene31724 | 0.800055 | 1.72E-48 | -1.69E-46 |
| MS.gene051666 | MS.gene31724 | 0.823752 | 1.17E-53 | -1.69E-46 |
| MS.gene051692 | MS.gene31724 | 0.857992 | 1.12E-62 | -1.69E-46 |
| MS.gene051810 | MS.gene31724 | 0.873504 | 1.40E-67 | -1.69E-46 |
| MS.gene051858 | MS.gene31724 | 0.828557 | 8.42E-55 | -1.69E-46 |
| MS.gene052078 | MS.gene31724 | 0.833513 | 5.11E-56 | -1.69E-46 |
| MS.gene052271 | MS.gene31724 | 0.876657 | 1.17E-68 | -1.69E-46 |
| MS.gene052290 | MS.gene31724 | 0.846986 | 1.54E-59 | -1.69E-46 |
| MS.gene052310 | MS.gene31724 | 0.828393 | 9.22E-55 | -1.69E-46 |
| MS.gene052322 | MS.gene31724 | 0.812751 | 3.62E-51 | -1.69E-46 |
| MS.gene052326 | MS.gene31724 | 0.800225 | 1.59E-48 | -1.69E-46 |
| MS.gene052340 | MS.gene31724 | 0.804838 | 1.78E-49 | -1.69E-46 |
| MS.gene052379 | MS.gene31724 | 0.854045 | 1.60E-61 | -1.69E-46 |
| MS.gene052421 | MS.gene31724 | 0.839373 | 1.65E-57 | -1.69E-46 |
| MS.gene052481 | MS.gene31724 | 0.811178 | 7.97E-51 | -1.69E-46 |
| MS.gene052484 | MS.gene31724 | 0.842334 | 2.76E-58 | -1.69E-46 |
| MS.gene052485 | MS.gene31724 | 0.819477 | 1.14E-52 | -1.69E-46 |
| MS.gene052508 | MS.gene31724 | 0.806984 | 6.31E-50 | -1.69E-46 |
| MS.gene052615 | MS.gene31724 | 0.856638 | 2.81E-62 | -1.69E-46 |
| MS.gene052916 | MS.gene31724 | 0.81418 | 1.76E-51 | -1.69E-46 |
| MS.gene053009 | MS.gene31724 | 0.850121 | 2.08E-60 | -1.69E-46 |
| MS.gene053103 | MS.gene31724 | 0.843337 | 1.49E-58 | -1.69E-46 |
| MS.gene053104 | MS.gene31724 | 0.853735 | 1.96E-61 | -1.69E-46 |
| MS.gene053207 | MS.gene31724 | 0.85014 | 2.06E-60 | -1.69E-46 |
| MS.gene053209 | MS.gene31724 | 0.880916 | 3.70E-70 | -1.69E-46 |
| MS.gene053213 | MS.gene31724 | 0.851512 | 8.46E-61 | -1.69E-46 |
| MS.gene053286 | MS.gene31724 | 0.838929 | 2.15E-57 | -1.69E-46 |
| MS.gene053322 | MS.gene31724 | 0.870986 | 9.64E-67 | -1.69E-46 |
| MS.gene053384 | MS.gene31724 | 0.82459 | 7.43E-54 | -1.69E-46 |
| MS.gene053449 | MS.gene31724 | 0.851023 | 1.16E-60 | -1.69E-46 |
| MS.gene053574 | MS.gene31724 | 0.809565 | 1.78E-50 | -1.69E-46 |
| MS.gene053632 | MS.gene31724 | 0.837351 | 5.47E-57 | -1.69E-46 |
| MS.gene053813 | MS.gene31724 | 0.858741 | 6.69E-63 | -1.69E-46 |
| MS.gene053916 | MS.gene31724 | 0.851956 | 6.33E-61 | -1.69E-46 |
| MS.gene054376 | MS.gene31724 | 0.879152 | 1.57E-69 | -1.69E-46 |
| MS.gene054543 | MS.gene31724 | 0.807764 | 4.31E-50 | -1.69E-46 |
| MS.gene054600 | MS.gene31724 | 0.832347 | 9.96E-56 | -1.69E-46 |
| MS.gene054642 | MS.gene31724 | 0.854331 | 1.32E-61 | -1.69E-46 |
| MS.gene054802 | MS.gene31724 | 0.853783 | 1.90E-61 | -1.69E-46 |
| MS.gene054803 | MS.gene31724 | 0.849865 | 2.46E-60 | -1.69E-46 |
| MS.gene054804 | MS.gene31724 | 0.858244 | 9.41E-63 | -1.69E-46 |
| MS.gene05505 | MS.gene31724 | 0.865377 | 6.17E-65 | -1.69E-46 |
| MS.gene055116 | MS.gene31724 | 0.821443 | 4.03E-53 | -1.69E-46 |
| MS.gene055206 | MS.gene31724 | 0.833116 | 6.42E-56 | -1.69E-46 |
| MS.gene055254 | MS.gene31724 | 0.871009 | 9.47E-67 | -1.69E-46 |
| MS.gene055277 | MS.gene31724 | 0.85886 | 6.16E-63 | -1.69E-46 |
| MS.gene055289 | MS.gene31724 | 0.80877 | 2.63E-50 | -1.69E-46 |
| MS.gene055362 | MS.gene31724 | 0.82607 | 3.32E-54 | -1.69E-46 |
| MS.gene055397 | MS.gene31724 | 0.879113 | 1.62E-69 | -1.69E-46 |
| MS.gene055412 | MS.gene31724 | 0.825027 | 5.86E-54 | -1.69E-46 |
| MS.gene055499 | MS.gene31724 | 0.879197 | 1.52E-69 | -1.69E-46 |
| MS.gene055636 | MS.gene31724 | 0.83308 | 6.55E-56 | -1.69E-46 |
| MS.gene055638 | MS.gene31724 | 0.816078 | 6.66E-52 | -1.69E-46 |
| MS.gene055647 | MS.gene31724 | 0.841214 | 5.44E-58 | -1.69E-46 |
| MS.gene055722 | MS.gene31724 | 0.869558 | 2.83E-66 | -1.69E-46 |
| MS.gene05592 | MS.gene31724 | 0.919816 | 2.80E-87 | -1.69E-46 |
| MS.gene055941 | MS.gene31724 | 0.821499 | 3.91E-53 | -1.69E-46 |
| MS.gene056191 | MS.gene31724 | 0.830585 | 2.70E-55 | -1.69E-46 |
| MS.gene056374 | MS.gene31724 | 0.867179 | 1.66E-65 | -1.69E-46 |
| MS.gene056410 | MS.gene31724 | 0.818369 | 2.03E-52 | -1.69E-46 |
| MS.gene056422 | MS.gene31724 | 0.809771 | 1.60E-50 | -1.69E-46 |
| MS.gene056487 | MS.gene31724 | 0.811303 | 7.49E-51 | -1.69E-46 |
| MS.gene056511 | MS.gene31724 | 0.811431 | 7.03E-51 | -1.69E-46 |
| MS.gene056609 | MS.gene31724 | 0.875512 | 2.91E-68 | -1.69E-46 |
| MS.gene056657 | MS.gene31724 | 0.834786 | 2.45E-56 | -1.69E-46 |
| MS.gene056897 | MS.gene31724 | 0.828901 | 6.95E-55 | -1.69E-46 |
| MS.gene057011 | MS.gene31724 | 0.830167 | 3.42E-55 | -1.69E-46 |
| MS.gene057076 | MS.gene31724 | 0.830319 | 3.14E-55 | -1.69E-46 |
| MS.gene057123 | MS.gene31724 | 0.804082 | 2.56E-49 | -1.69E-46 |
| MS.gene057124 | MS.gene31724 | 0.834542 | 2.82E-56 | -1.69E-46 |
| MS.gene057129 | MS.gene31724 | 0.905446 | 4.25E-80 | -1.69E-46 |
| MS.gene057146 | MS.gene31724 | 0.814913 | 1.21E-51 | -1.69E-46 |
| MS.gene057295 | MS.gene31724 | 0.82677 | 2.26E-54 | -1.69E-46 |
| MS.gene057331 | MS.gene31724 | 0.869872 | 2.24E-66 | -1.69E-46 |
| MS.gene05733 | MS.gene31724 | 0.802386 | 5.75E-49 | -1.69E-46 |
| MS.gene05735 | MS.gene31724 | 0.853692 | 2.02E-61 | -1.69E-46 |
| MS.gene057416 | MS.gene31724 | 0.853903 | 1.76E-61 | -1.69E-46 |
| MS.gene057926 | MS.gene31724 | 0.840841 | 6.82E-58 | -1.69E-46 |
| MS.gene058006 | MS.gene31724 | 0.874354 | 7.21E-68 | -1.69E-46 |
| MS.gene058019 | MS.gene31724 | 0.828126 | 1.07E-54 | -1.69E-46 |
| MS.gene058130 | MS.gene31724 | 0.811448 | 6.96E-51 | -1.69E-46 |
| MS.gene058293 | MS.gene31724 | 0.870912 | 1.02E-66 | -1.69E-46 |
| MS.gene058323 | MS.gene31724 | 0.855569 | 5.78E-62 | -1.69E-46 |
| MS.gene058365 | MS.gene31724 | 0.809866 | 1.53E-50 | -1.69E-46 |
| MS.gene058419 | MS.gene31724 | 0.839255 | 1.77E-57 | -1.69E-46 |
| MS.gene058660 | MS.gene31724 | 0.840917 | 6.52E-58 | -1.69E-46 |
| MS.gene058723 | MS.gene31724 | 0.848432 | 6.15E-60 | -1.69E-46 |
| MS.gene058903 | MS.gene31724 | 0.803454 | 3.46E-49 | -1.69E-46 |
| MS.gene058928 | MS.gene31724 | 0.824086 | 9.77E-54 | -1.69E-46 |
| MS.gene059022 | MS.gene31724 | 0.804058 | 2.59E-49 | -1.69E-46 |
| MS.gene059029 | MS.gene31724 | 0.841141 | 5.69E-58 | -1.69E-46 |
| MS.gene059326 | MS.gene31724 | 0.820827 | 5.58E-53 | -1.69E-46 |
| MS.gene059366 | MS.gene31724 | 0.818234 | 2.18E-52 | -1.69E-46 |
| MS.gene059536 | MS.gene31724 | 0.832593 | 8.65E-56 | -1.69E-46 |
| MS.gene059676 | MS.gene31724 | 0.824043 | 9.99E-54 | -1.69E-46 |
| MS.gene059677 | MS.gene31724 | 0.814884 | 1.23E-51 | -1.69E-46 |
| MS.gene059688 | MS.gene31724 | 0.859962 | 2.88E-63 | -1.69E-46 |
| MS.gene059692 | MS.gene31724 | 0.876054 | 1.89E-68 | -1.69E-46 |
| MS.gene059695 | MS.gene31724 | 0.869704 | 2.54E-66 | -1.69E-46 |
| MS.gene059711 | MS.gene31724 | 0.830615 | 2.66E-55 | -1.69E-46 |
| MS.gene059718 | MS.gene31724 | 0.821916 | 3.13E-53 | -1.69E-46 |
| MS.gene059858 | MS.gene31724 | 0.805283 | 1.44E-49 | -1.69E-46 |
| MS.gene059894 | MS.gene31724 | 0.817039 | 4.06E-52 | -1.69E-46 |
| MS.gene059902 | MS.gene31724 | 0.825079 | 5.70E-54 | -1.69E-46 |
| MS.gene059960 | MS.gene31724 | 0.870001 | 2.03E-66 | -1.69E-46 |
| MS.gene059974 | MS.gene31724 | 0.830585 | 2.70E-55 | -1.69E-46 |
| MS.gene060005 | MS.gene31724 | 0.808113 | 3.63E-50 | -1.69E-46 |
| MS.gene060193 | MS.gene31724 | 0.83746 | 5.13E-57 | -1.69E-46 |
| MS.gene060356 | MS.gene31724 | 0.805265 | 1.45E-49 | -1.69E-46 |
| MS.gene06037 | MS.gene31724 | 0.842833 | 2.03E-58 | -1.69E-46 |
| MS.gene060403 | MS.gene31724 | 0.803417 | 3.52E-49 | -1.69E-46 |
| MS.gene060804 | MS.gene31724 | 0.869156 | 3.82E-66 | -1.69E-46 |
| MS.gene060818 | MS.gene31724 | 0.868614 | 5.73E-66 | -1.69E-46 |
| MS.gene060835 | MS.gene31724 | 0.825706 | 4.05E-54 | -1.69E-46 |
| MS.gene060837 | MS.gene31724 | 0.832463 | 9.32E-56 | -1.69E-46 |
| MS.gene06092 | MS.gene31724 | 0.814189 | 1.75E-51 | -1.69E-46 |
| MS.gene061041 | MS.gene31724 | 0.856395 | 3.31E-62 | -1.69E-46 |
| MS.gene061049 | MS.gene31724 | 0.813244 | 2.82E-51 | -1.69E-46 |
| MS.gene061051 | MS.gene31724 | -0.83679 | 7.61E-57 | -1.69E-46 |
| MS.gene061134 | MS.gene31724 | 0.803759 | 2.99E-49 | -1.69E-46 |
| MS.gene061150 | MS.gene31724 | 0.807793 | 4.25E-50 | -1.69E-46 |
| MS.gene061200 | MS.gene31724 | 0.876973 | 9.11E-69 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene31724.t1 | MTR_1g026820 | 96.923 | 130 | 4 | 0 | 1 | 130 | 1 | 130 | 3.76e-87 | 249 |
| MS.gene31724.t1 | MTR_1g026820 | 97.959 | 98 | 2 | 0 | 33 | 130 | 1 | 98 | 3.99e-64 | 190 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene31724.t1 | AT1G30380 | 81.679 | 131 | 22 | 2 | 1 | 130 | 1 | 130 | 1.29e-72 | 213 |
Find 52 sgRNAs with CRISPR-Local
Find 68 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATTGGTGTTGGTGTTGTTCT+TGG | 0.264103 | 1.1:-12917195 | MS.gene31724:CDS |
| GCGCAAAGGCAAGGGTGGTT+TGG | 0.284320 | 1.1:-12917740 | MS.gene31724:CDS |
| ATCTTCCAGCAAACAACATT+AGG | 0.298751 | 1.1:+12917355 | None:intergenic |
| CGCAAAGGCAAGGGTGGTTT+GGG | 0.299534 | 1.1:-12917739 | MS.gene31724:CDS |
| TTGCCTTTGCGCCTTGTCAT+AGG | 0.312155 | 1.1:+12917751 | None:intergenic |
| GTTCACCCTTGCTGATACTT+TGG | 0.359682 | 1.1:-12917241 | MS.gene31724:CDS |
| TTGCAGACTGGTGACCCAGC+TGG | 0.370604 | 1.1:-12917264 | MS.gene31724:CDS |
| ACCTTCTCCTATCCAGGGTT+TGG | 0.377236 | 1.1:-12918017 | MS.gene31724:intron |
| ATGTTGTTTGCTGGAAGATT+TGG | 0.379579 | 1.1:-12917351 | MS.gene31724:CDS |
| TTGAAATCATACCACATTAG+TGG | 0.382365 | 1.1:+12917690 | None:intergenic |
| GGAGTTCGTTGTGACTTCAT+TGG | 0.392047 | 1.1:-12917718 | MS.gene31724:CDS |
| TCTTCCAGCAAACAACATTA+GGG | 0.393888 | 1.1:+12917356 | None:intergenic |
| ACAACCCTAATGTTGTTTGC+TGG | 0.398888 | 1.1:-12917360 | MS.gene31724:CDS |
| TGCAGACTGGTGACCCAGCT+GGG | 0.430536 | 1.1:-12917263 | MS.gene31724:CDS |
| CTTTGCGCCTTGTCATAGGT+TGG | 0.430581 | 1.1:+12917755 | None:intergenic |
| ACTCAAACCTTCTCCTATCC+AGG | 0.437202 | 1.1:-12918023 | MS.gene31724:CDS |
| TCAGCAAGGGTGAACCCAGC+TGG | 0.438597 | 1.1:+12917249 | None:intergenic |
| GATTGGCTCCCTCAGCAAAC+AGG | 0.449260 | 1.1:-12917329 | MS.gene31724:CDS |
| TTTGCGCCTTGTCATAGGTT+GGG | 0.452938 | 1.1:+12917756 | None:intergenic |
| ACTTTGGCCTGTGGGACTGT+TGG | 0.461540 | 1.1:-12917225 | MS.gene31724:CDS |
| ACAACTTTGCCTCAATTCAC+TGG | 0.462189 | 1.1:-12918058 | MS.gene31724:CDS |
| AGCAACAGCAGGACTAAAGC+TGG | 0.487183 | 1.1:-12917304 | MS.gene31724:CDS |
| GTTGCTTTCCTGTTTGCTGA+GGG | 0.491197 | 1.1:+12917321 | None:intergenic |
| GTTTGCTGGAAGATTTGGAT+TGG | 0.494741 | 1.1:-12917346 | MS.gene31724:CDS |
| TTGCTGATACTTTGGCCTGT+GGG | 0.511025 | 1.1:-12917233 | MS.gene31724:CDS |
| CTTGCTGATACTTTGGCCTG+TGG | 0.513317 | 1.1:-12917234 | MS.gene31724:CDS |
| TATGACAAGGCGCAAAGGCA+AGG | 0.517020 | 1.1:-12917749 | MS.gene31724:CDS |
| GGGACTGTTGGACACATCAT+TGG | 0.518734 | 1.1:-12917213 | MS.gene31724:CDS |
| GTTCTTGGTCTTAAGAACAT+TGG | 0.536798 | 1.1:-12917180 | MS.gene31724:CDS |
| AGGGACTCAGGCTTGCAGAC+TGG | 0.536809 | 1.1:-12917276 | MS.gene31724:CDS |
| CAGGACTAAAGCTGGAGACA+AGG | 0.539368 | 1.1:-12917296 | MS.gene31724:CDS |
| TGTTGCTTTCCTGTTTGCTG+AGG | 0.553786 | 1.1:+12917320 | None:intergenic |
| ACCAAACCCTGGATAGGAGA+AGG | 0.553964 | 1.1:+12918016 | None:intergenic |
| GTTGGACACATCATTGGTGT+TGG | 0.557734 | 1.1:-12917207 | MS.gene31724:CDS |
| AAGCTGGAGACAAGGGACTC+AGG | 0.559360 | 1.1:-12917288 | MS.gene31724:CDS |
| GATGTGTCCAACAGTCCCAC+AGG | 0.566507 | 1.1:+12917218 | None:intergenic |
| CAGCAAGGGTGAACCCAGCT+GGG | 0.567280 | 1.1:+12917250 | None:intergenic |
| ATGACAAGGCGCAAAGGCAA+GGG | 0.568554 | 1.1:-12917748 | MS.gene31724:CDS |
| ATAGGAGAAGGTTTGAGTTG+TGG | 0.576762 | 1.1:+12918028 | None:intergenic |
| TTAATTACCAAACCCTGGAT+AGG | 0.584959 | 1.1:+12918010 | None:intergenic |
| CATTGGTTCATCCACTAATG+TGG | 0.586749 | 1.1:-12917701 | MS.gene31724:intron |
| AGGACTAAAGCTGGAGACAA+GGG | 0.595838 | 1.1:-12917295 | MS.gene31724:CDS |
| CTCAAACCTTCTCCTATCCA+GGG | 0.595941 | 1.1:-12918022 | MS.gene31724:CDS |
| CACAGGCCAAAGTATCAGCA+AGG | 0.601389 | 1.1:+12917235 | None:intergenic |
| AGTGGTTAATTACCAAACCC+TGG | 0.602633 | 1.1:+12918005 | None:intergenic |
| GGTCTAAGACCAGTGAATTG+AGG | 0.606924 | 1.1:+12918049 | None:intergenic |
| GCAAACAGGAAAGCAACAGC+AGG | 0.611764 | 1.1:-12917315 | MS.gene31724:CDS |
| CAACCTATGACAAGGCGCAA+AGG | 0.611923 | 1.1:-12917754 | MS.gene31724:CDS |
| ACAAGGCGCAAAGGCAAGGG+TGG | 0.641021 | 1.1:-12917745 | MS.gene31724:CDS |
| ACAGGCCAAAGTATCAGCAA+GGG | 0.672894 | 1.1:+12917236 | None:intergenic |
| TTGCTGCCCAACCTATGACA+AGG | 0.674324 | 1.1:-12917762 | MS.gene31724:CDS |
| ACATGTTGGACAGATTATGG+TGG | 0.710941 | 1.1:-12917388 | MS.gene31724:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTTAAATATATATAGTATGA+AGG | + | chr1.1:12917411-12917430 | None:intergenic | 10.0% |
| !!! | TTTAAGTTTTATAATTCATA+AGG | + | chr1.1:12917375-12917394 | None:intergenic | 10.0% |
| !!! | TGTTTATTTTTTTTATGTGA+AGG | - | chr1.1:12917461-12917480 | MS.gene31724:intron | 15.0% |
| !! | AAAACTATACATATCATGAT+TGG | + | chr1.1:12917737-12917756 | None:intergenic | 20.0% |
| !! | ATGAATAAAAACATTGGAAT+TGG | + | chr1.1:12917591-12917610 | None:intergenic | 20.0% |
| !! | ATTCATATTTGTAAATCGTA+TGG | + | chr1.1:12917343-12917362 | None:intergenic | 20.0% |
| !! | TATTCTATATACTTACTTCT+TGG | - | chr1.1:12917316-12917335 | MS.gene31724:CDS | 20.0% |
| ! | AAGTTACAAATTCTAACAAG+TGG | + | chr1.1:12917806-12917825 | None:intergenic | 25.0% |
| AAATGATGACATGAAAAGAG+TGG | + | chr1.1:12917262-12917281 | None:intergenic | 30.0% | |
| AAGCATAGTGTCACAATAAT+TGG | + | chr1.1:12917765-12917784 | None:intergenic | 30.0% | |
| CGCACTATGAATAAAAACAT+TGG | + | chr1.1:12917597-12917616 | None:intergenic | 30.0% | |
| TAACGAGTTTCGCAAAATTA+CGG | + | chr1.1:12917712-12917731 | None:intergenic | 30.0% | |
| TTGAAATCATACCACATTAG+TGG | + | chr1.1:12917559-12917578 | None:intergenic | 30.0% | |
| ! | TATGATTGACTTTGACATGT+TGG | - | chr1.1:12917844-12917863 | MS.gene31724:intron | 30.0% |
| ATCTTCCAGCAAACAACATT+AGG | + | chr1.1:12917894-12917913 | None:intergenic | 35.0% | |
| TCTTCCAGCAAACAACATTA+GGG | + | chr1.1:12917893-12917912 | None:intergenic | 35.0% | |
| TTAATTACCAAACCCTGGAT+AGG | + | chr1.1:12917239-12917258 | None:intergenic | 35.0% | |
| TTGACATGTTGGACAGATTA+TGG | - | chr1.1:12917855-12917874 | MS.gene31724:intron | 35.0% | |
| ! | ATGTTGTTTGCTGGAAGATT+TGG | - | chr1.1:12917895-12917914 | MS.gene31724:intron | 35.0% |
| ! | TTTTATTCATAGTGCGTGTC+TGG | - | chr1.1:12917601-12917620 | MS.gene31724:intron | 35.0% |
| !! | GTTCTTGGTCTTAAGAACAT+TGG | - | chr1.1:12918066-12918085 | MS.gene31724:CDS | 35.0% |
| ACAACTTTGCCTCAATTCAC+TGG | - | chr1.1:12917188-12917207 | MS.gene31724:CDS | 40.0% | |
| ACATGTTGGACAGATTATGG+TGG | - | chr1.1:12917858-12917877 | MS.gene31724:intron | 40.0% | |
| AGTGGTTAATTACCAAACCC+TGG | + | chr1.1:12917244-12917263 | None:intergenic | 40.0% | |
| ! | ACAACCCTAATGTTGTTTGC+TGG | - | chr1.1:12917886-12917905 | MS.gene31724:intron | 40.0% |
| ! | GTTTGCTGGAAGATTTGGAT+TGG | - | chr1.1:12917900-12917919 | MS.gene31724:intron | 40.0% |
| !! | ATAGGAGAAGGTTTGAGTTG+TGG | + | chr1.1:12917221-12917240 | None:intergenic | 40.0% |
| !! | ATTGGTGTTGGTGTTGTTCT+TGG | - | chr1.1:12918051-12918070 | MS.gene31724:CDS | 40.0% |
| !! | CATTGGTTCATCCACTAATG+TGG | - | chr1.1:12917545-12917564 | MS.gene31724:intron | 40.0% |
| ACTCAAACCTTCTCCTATCC+AGG | - | chr1.1:12917223-12917242 | MS.gene31724:CDS | 45.0% | |
| AGGACTAAAGCTGGAGACAA+GGG | - | chr1.1:12917951-12917970 | MS.gene31724:intron | 45.0% | |
| CTCAAACCTTCTCCTATCCA+GGG | - | chr1.1:12917224-12917243 | MS.gene31724:CDS | 45.0% | |
| GGAGTTCGTTGTGACTTCAT+TGG | - | chr1.1:12917528-12917547 | MS.gene31724:intron | 45.0% | |
| GTTCACCCTTGCTGATACTT+TGG | - | chr1.1:12918005-12918024 | MS.gene31724:intron | 45.0% | |
| GTTGGACACATCATTGGTGT+TGG | - | chr1.1:12918039-12918058 | MS.gene31724:CDS | 45.0% | |
| TTGCTGATACTTTGGCCTGT+GGG | - | chr1.1:12918013-12918032 | MS.gene31724:intron | 45.0% | |
| TTTGCGCCTTGTCATAGGTT+GGG | + | chr1.1:12917493-12917512 | None:intergenic | 45.0% | |
| ! | ACAGGCCAAAGTATCAGCAA+GGG | + | chr1.1:12918013-12918032 | None:intergenic | 45.0% |
| ! | GGTCTAAGACCAGTGAATTG+AGG | + | chr1.1:12917200-12917219 | None:intergenic | 45.0% |
| ! | GTTGCTTTCCTGTTTGCTGA+GGG | + | chr1.1:12917928-12917947 | None:intergenic | 45.0% |
| !! | TGTTGCTTTCCTGTTTGCTG+AGG | + | chr1.1:12917929-12917948 | None:intergenic | 45.0% |
| !!! | TACTATATATATTTAAATTT+TGG | - | chr1.1:12917414-12917433 | MS.gene31724:intron | 5.0% |
| ACCAAACCCTGGATAGGAGA+AGG | + | chr1.1:12917233-12917252 | None:intergenic | 50.0% | |
| ACCTTCTCCTATCCAGGGTT+TGG | - | chr1.1:12917229-12917248 | MS.gene31724:CDS | 50.0% | |
| AGCAACAGCAGGACTAAAGC+TGG | - | chr1.1:12917942-12917961 | MS.gene31724:intron | 50.0% | |
| ATGACAAGGCGCAAAGGCAA+GGG | - | chr1.1:12917498-12917517 | MS.gene31724:intron | 50.0% | |
| CAACCTATGACAAGGCGCAA+AGG | - | chr1.1:12917492-12917511 | MS.gene31724:intron | 50.0% | |
| CAGGACTAAAGCTGGAGACA+AGG | - | chr1.1:12917950-12917969 | MS.gene31724:intron | 50.0% | |
| CTTGCTGATACTTTGGCCTG+TGG | - | chr1.1:12918012-12918031 | MS.gene31724:intron | 50.0% | |
| CTTTGCGCCTTGTCATAGGT+TGG | + | chr1.1:12917494-12917513 | None:intergenic | 50.0% | |
| GCAAACAGGAAAGCAACAGC+AGG | - | chr1.1:12917931-12917950 | MS.gene31724:intron | 50.0% | |
| GGGACTGTTGGACACATCAT+TGG | - | chr1.1:12918033-12918052 | MS.gene31724:CDS | 50.0% | |
| TATGACAAGGCGCAAAGGCA+AGG | - | chr1.1:12917497-12917516 | MS.gene31724:intron | 50.0% | |
| TTGCCTTTGCGCCTTGTCAT+AGG | + | chr1.1:12917498-12917517 | None:intergenic | 50.0% | |
| TTGCTGCCCAACCTATGACA+AGG | - | chr1.1:12917484-12917503 | MS.gene31724:intron | 50.0% | |
| ! | CACAGGCCAAAGTATCAGCA+AGG | + | chr1.1:12918014-12918033 | None:intergenic | 50.0% |
| AAGCTGGAGACAAGGGACTC+AGG | - | chr1.1:12917958-12917977 | MS.gene31724:intron | 55.0% | |
| ACTTTGGCCTGTGGGACTGT+TGG | - | chr1.1:12918021-12918040 | MS.gene31724:CDS | 55.0% | |
| CGCAAAGGCAAGGGTGGTTT+GGG | - | chr1.1:12917507-12917526 | MS.gene31724:intron | 55.0% | |
| GATGTGTCCAACAGTCCCAC+AGG | + | chr1.1:12918031-12918050 | None:intergenic | 55.0% | |
| ! | GATTGGCTCCCTCAGCAAAC+AGG | - | chr1.1:12917917-12917936 | MS.gene31724:intron | 55.0% |
| ACAAGGCGCAAAGGCAAGGG+TGG | - | chr1.1:12917501-12917520 | MS.gene31724:intron | 60.0% | |
| AGGGACTCAGGCTTGCAGAC+TGG | - | chr1.1:12917970-12917989 | MS.gene31724:intron | 60.0% | |
| CAGCAAGGGTGAACCCAGCT+GGG | + | chr1.1:12917999-12918018 | None:intergenic | 60.0% | |
| GCGCAAAGGCAAGGGTGGTT+TGG | - | chr1.1:12917506-12917525 | MS.gene31724:intron | 60.0% | |
| TCAGCAAGGGTGAACCCAGC+TGG | + | chr1.1:12918000-12918019 | None:intergenic | 60.0% | |
| TGCAGACTGGTGACCCAGCT+GGG | - | chr1.1:12917983-12918002 | MS.gene31724:intron | 60.0% | |
| TTGCAGACTGGTGACCCAGC+TGG | - | chr1.1:12917982-12918001 | MS.gene31724:intron | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.1 | gene | 12917170 | 12918098 | 12917170 | ID=MS.gene31724 |
| chr1.1 | mRNA | 12917170 | 12918098 | 12917170 | ID=MS.gene31724.t1;Parent=MS.gene31724 |
| chr1.1 | exon | 12918018 | 12918098 | 12918018 | ID=MS.gene31724.t1.exon1;Parent=MS.gene31724.t1 |
| chr1.1 | CDS | 12918018 | 12918098 | 12918018 | ID=cds.MS.gene31724.t1;Parent=MS.gene31724.t1 |
| chr1.1 | exon | 12917702 | 12917785 | 12917702 | ID=MS.gene31724.t1.exon2;Parent=MS.gene31724.t1 |
| chr1.1 | CDS | 12917702 | 12917785 | 12917702 | ID=cds.MS.gene31724.t1;Parent=MS.gene31724.t1 |
| chr1.1 | exon | 12917170 | 12917397 | 12917170 | ID=MS.gene31724.t1.exon3;Parent=MS.gene31724.t1 |
| chr1.1 | CDS | 12917170 | 12917397 | 12917170 | ID=cds.MS.gene31724.t1;Parent=MS.gene31724.t1 |
| Gene Sequence |
| Protein sequence |
