AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene80970


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene80970.t1 MTR_7g007120 69.006 171 8 1 11 136 11 181 4.36e-80 234
MS.gene80970.t1 MTR_7g007200 68.421 171 9 1 11 136 11 181 6.55e-80 233
MS.gene80970.t1 MTR_7g007220 69.006 171 8 1 11 136 11 181 9.50e-80 233
MS.gene80970.t1 MTR_7g007120 68.824 170 8 1 12 136 12 181 1.85e-79 232
MS.gene80970.t1 MTR_7g007120 68.421 171 9 1 11 136 11 181 2.07e-79 232
MS.gene80970.t1 MTR_7g007230 67.059 170 11 1 12 136 12 181 6.88e-77 226
MS.gene80970.t1 MTR_6g018310 62.570 179 21 2 4 136 2 180 7.13e-73 216
MS.gene80970.t1 MTR_6g018300 62.570 179 18 3 4 136 2 177 9.13e-71 210
MS.gene80970.t1 MTR_7g007210 66.234 154 7 1 11 119 11 164 3.22e-66 199
MS.gene80970.t1 MTR_4g127870 52.703 74 35 0 62 135 96 169 5.39e-29 104
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene80970.t1 AT5G38410 52.198 182 37 3 1 136 1 178 5.91e-57 175
MS.gene80970.t1 AT1G67090 52.198 182 37 3 1 136 1 178 1.47e-56 174
MS.gene80970.t1 AT5G38430 52.198 182 37 3 1 136 1 178 1.55e-56 174
MS.gene80970.t1 AT5G38430 52.198 182 37 3 1 136 1 178 1.55e-56 174
MS.gene80970.t1 AT5G38420 52.198 182 37 3 1 136 1 178 2.70e-56 174
MS.gene80970.t1 AT5G38410 50.802 187 37 3 1 136 1 183 5.45e-56 173
MS.gene80970.t1 AT5G38410 52.000 175 41 4 1 136 1 171 4.14e-53 165
MS.gene80970.t1 AT1G67090 41.667 132 55 8 1 131 1 111 1.01e-15 69.3

Find 38 sgRNAs with CRISPR-Local

Find 65 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTTTGTTGATCTTTGTAAC+TGG 0.311725 7.3:+90835748 None:intergenic
GGCGCGGTGGCTCCATTTGT+TGG 0.347642 7.3:-90835793 MS.gene80970:CDS
GATACTATGATGGACGTTAC+TGG 0.371866 7.3:-90835233 MS.gene80970:CDS
CGATTCTTCTCAAGTGTTGA+AGG 0.396980 7.3:-90835178 MS.gene80970:CDS
TCTTCCACCATTGCTTGTAA+TGG 0.401668 7.3:+90835717 None:intergenic
ATGTGGAAGTTGCCTTTGTT+TGG 0.411503 7.3:-90835207 MS.gene80970:CDS
GTTGGACTCAAGTCCATGGC+TGG 0.431505 7.3:-90835775 MS.gene80970:CDS
TACAGTTAACCGTGCCTCTT+CGG 0.432912 7.3:-90835824 MS.gene80970:CDS
ATGATACGGACAAAGGCATC+AGG 0.443523 7.3:+90835129 None:intergenic
CTTCAACACTTGAGAAGAAT+CGG 0.461194 7.3:+90835179 None:intergenic
GATGCCTTTGTCCGTATCAT+CGG 0.484184 7.3:-90835126 MS.gene80970:CDS
CTTTGTTGATCTTTGTAACT+GGG 0.495423 7.3:+90835749 None:intergenic
ATTTGTTGGACTCAAGTCCA+TGG 0.520007 7.3:-90835779 MS.gene80970:CDS
CGTGCCTCTTCGGTACAATC+CGG 0.524408 7.3:-90835814 MS.gene80970:CDS
ATGGACTTGAGTCCAACAAA+TGG 0.526746 7.3:+90835781 None:intergenic
TCCACCATTGCTTGTAATGG+AGG 0.530318 7.3:+90835720 None:intergenic
AGCTGAAGAGGAAATTATAG+AGG 0.539718 7.3:+90835852 None:intergenic
CATAGTATCCTGGTGACTTG+TGG 0.542155 7.3:+90835247 None:intergenic
CGTGAGAACCACAAGTCACC+AGG 0.544938 7.3:-90835255 MS.gene80970:CDS
AACAAATGGAGCCACCGCGC+CGG 0.560979 7.3:+90835795 None:intergenic
TAACGTCCATCATAGTATCC+TGG 0.569613 7.3:+90835237 None:intergenic
AAGTCACCAGGATACTATGA+TGG 0.574009 7.3:-90835243 MS.gene80970:CDS
CGTTGTCGAATCCGATGATA+CGG 0.579522 7.3:+90835115 None:intergenic
ACTCAAACTTAGTAAACTGC+AGG 0.582384 7.3:+90835054 None:intergenic
AACTGTAGTCACAGCTGAAG+AGG 0.589798 7.3:+90835840 None:intergenic
CTGGTGACTTGTGGTTCTCA+CGG 0.595328 7.3:+90835256 None:intergenic
GAATCGGTTGCTCCAAACAA+AGG 0.598394 7.3:+90835195 None:intergenic
ATTACCTCCATTACAAGCAA+TGG 0.604904 7.3:-90835724 MS.gene80970:CDS
ACCTCCATTACAAGCAATGG+TGG 0.613355 7.3:-90835721 MS.gene80970:CDS
TTCGGTACAATCCGGCGCGG+TGG 0.614407 7.3:-90835806 MS.gene80970:CDS
TGTAACTGGGAAGCCAGCCA+TGG 0.615947 7.3:+90835762 None:intergenic
CGGATTGTACCGAAGAGGCA+CGG 0.641462 7.3:+90835815 None:intergenic
TGGAAGAGTAAACTGCATGC+AGG 0.652563 7.3:-90835701 MS.gene80970:intron
TGATACGGACAAAGGCATCA+GGG 0.656050 7.3:+90835130 None:intergenic
CGCGCCGGATTGTACCGAAG+AGG 0.661856 7.3:+90835810 None:intergenic
GAATCCGATGATACGGACAA+AGG 0.687909 7.3:+90835122 None:intergenic
CTCTTCGGTACAATCCGGCG+CGG 0.696951 7.3:-90835809 MS.gene80970:CDS
ATGGACGTTACTGGACAATG+TGG 0.742197 7.3:-90835224 MS.gene80970:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TCAAATAAACTAAAATAGTA+GGG + chr7.3:90835261-90835280 None:intergenic 15.0%
!! CTCAAATAAACTAAAATAGT+AGG + chr7.3:90835262-90835281 None:intergenic 20.0%
!!! CTACTATTTTAGTTTATTTG+AGG - chr7.3:90835260-90835279 MS.gene80970:CDS 20.0%
! AGTAAAGTAATATTAGACCT+CGG + chr7.3:90835522-90835541 None:intergenic 25.0%
!! AAGAAGTTGATTACCTTATA+AGG - chr7.3:90835465-90835484 MS.gene80970:intron 25.0%
!! TAACCATTTTGTGCAATTTT+AGG - chr7.3:90835363-90835382 MS.gene80970:intron 25.0%
!!! TGTTTGTTTTGATTTGTACT+TGG - chr7.3:90835618-90835637 MS.gene80970:intron 25.0%
ACACCTAAAATTGCACAAAA+TGG + chr7.3:90835369-90835388 None:intergenic 30.0%
AGTTGATTACCTTATAAGGA+AGG - chr7.3:90835469-90835488 MS.gene80970:intron 30.0%
ATAAGCACTTATAGATTCGT+TGG - chr7.3:90835321-90835340 MS.gene80970:intron 30.0%
CTTTGTTGATCTTTGTAACT+GGG + chr7.3:90835175-90835194 None:intergenic 30.0%
GTTGATTACCTTATAAGGAA+GGG - chr7.3:90835470-90835489 MS.gene80970:intron 30.0%
TCTTTGTTGATCTTTGTAAC+TGG + chr7.3:90835176-90835195 None:intergenic 30.0%
!! ATTTTGTGCAATTTTAGGTG+TGG - chr7.3:90835368-90835387 MS.gene80970:intron 30.0%
AGCTGAAGAGGAAATTATAG+AGG + chr7.3:90835072-90835091 None:intergenic 35.0%
ATTACCTCCATTACAAGCAA+TGG - chr7.3:90835197-90835216 MS.gene80970:CDS 35.0%
ATTACCTTATAAGGAAGGGA+TGG - chr7.3:90835474-90835493 MS.gene80970:intron 35.0%
CTTCAACACTTGAGAAGAAT+CGG + chr7.3:90835745-90835764 None:intergenic 35.0%
TCAAACTTCTTCTTGCCAAT+AGG + chr7.3:90835397-90835416 None:intergenic 35.0%
TTACCTTATAAGGAAGGGAT+GGG - chr7.3:90835475-90835494 MS.gene80970:intron 35.0%
TTCTTTAGCCAATTGTTCTC+TGG + chr7.3:90835450-90835469 None:intergenic 35.0%
AACTTCTTCTTGCCAATAGG+AGG + chr7.3:90835394-90835413 None:intergenic 40.0%
AAGTCACCAGGATACTATGA+TGG - chr7.3:90835678-90835697 MS.gene80970:intron 40.0%
AATTGTTCTCTGGTCAATGG+TGG + chr7.3:90835440-90835459 None:intergenic 40.0%
ACCATTGACCAGAGAACAAT+TGG - chr7.3:90835439-90835458 MS.gene80970:intron 40.0%
ATGGACTTGAGTCCAACAAA+TGG + chr7.3:90835143-90835162 None:intergenic 40.0%
ATGTGGAAGTTGCCTTTGTT+TGG - chr7.3:90835714-90835733 MS.gene80970:CDS 40.0%
CAAATTTGTGACTGTGACCT+TGG + chr7.3:90835587-90835606 None:intergenic 40.0%
GTACTTGGTTACTTGCAGAA+AGG - chr7.3:90835633-90835652 MS.gene80970:intron 40.0%
TAACGTCCATCATAGTATCC+TGG + chr7.3:90835687-90835706 None:intergenic 40.0%
TCTTCCACCATTGCTTGTAA+TGG + chr7.3:90835207-90835226 None:intergenic 40.0%
! ATTTGTTGGACTCAAGTCCA+TGG - chr7.3:90835142-90835161 MS.gene80970:CDS 40.0%
!! CGATTCTTCTCAAGTGTTGA+AGG - chr7.3:90835743-90835762 MS.gene80970:CDS 40.0%
!! GATACTATGATGGACGTTAC+TGG - chr7.3:90835688-90835707 MS.gene80970:intron 40.0%
AACTGTAGTCACAGCTGAAG+AGG + chr7.3:90835084-90835103 None:intergenic 45.0%
ACCTCCATTACAAGCAATGG+TGG - chr7.3:90835200-90835219 MS.gene80970:CDS 45.0%
ATGGACGTTACTGGACAATG+TGG - chr7.3:90835697-90835716 MS.gene80970:intron 45.0%
CAACCCATCCCTTCCTTATA+AGG + chr7.3:90835481-90835500 None:intergenic 45.0%
CATAGTATCCTGGTGACTTG+TGG + chr7.3:90835677-90835696 None:intergenic 45.0%
CGTTGTCGAATCCGATGATA+CGG + chr7.3:90835809-90835828 None:intergenic 45.0%
GAATCCGATGATACGGACAA+AGG + chr7.3:90835802-90835821 None:intergenic 45.0%
GAATCGGTTGCTCCAAACAA+AGG + chr7.3:90835729-90835748 None:intergenic 45.0%
GATGCCTTTGTCCGTATCAT+CGG - chr7.3:90835795-90835814 MS.gene80970:CDS 45.0%
GCCAATTGTTCTCTGGTCAA+TGG + chr7.3:90835443-90835462 None:intergenic 45.0%
TACAGTTAACCGTGCCTCTT+CGG - chr7.3:90835097-90835116 MS.gene80970:CDS 45.0%
TCCACCATTGCTTGTAATGG+AGG + chr7.3:90835204-90835223 None:intergenic 45.0%
TGGAAGAGTAAACTGCATGC+AGG - chr7.3:90835220-90835239 MS.gene80970:CDS 45.0%
! TTGCTTGGAATTTGAGACCG+AGG - chr7.3:90835502-90835521 MS.gene80970:intron 45.0%
!! ATGATACGGACAAAGGCATC+AGG + chr7.3:90835795-90835814 None:intergenic 45.0%
!! TGATACGGACAAAGGCATCA+GGG + chr7.3:90835794-90835813 None:intergenic 45.0%
!! TTTTAGGTGTGGCCTCCTAT+TGG - chr7.3:90835379-90835398 MS.gene80970:intron 45.0%
! CTGGTGACTTGTGGTTCTCA+CGG + chr7.3:90835668-90835687 None:intergenic 50.0%
! GTTCTCTGGTCAATGGTGGA+AGG + chr7.3:90835436-90835455 None:intergenic 50.0%
!! ACGTTGTTTTGTCGCGACCA+AGG - chr7.3:90835567-90835586 MS.gene80970:intron 50.0%
CGGATTGTACCGAAGAGGCA+CGG + chr7.3:90835109-90835128 None:intergenic 55.0%
CGTGAGAACCACAAGTCACC+AGG - chr7.3:90835666-90835685 MS.gene80970:intron 55.0%
CGTGCCTCTTCGGTACAATC+CGG - chr7.3:90835107-90835126 MS.gene80970:CDS 55.0%
GTTGGACTCAAGTCCATGGC+TGG - chr7.3:90835146-90835165 MS.gene80970:CDS 55.0%
TGTAACTGGGAAGCCAGCCA+TGG + chr7.3:90835162-90835181 None:intergenic 55.0%
!! GAAGGGATGGGTTGCTTGCT+TGG - chr7.3:90835487-90835506 MS.gene80970:intron 55.0%
AACAAATGGAGCCACCGCGC+CGG + chr7.3:90835129-90835148 None:intergenic 60.0%
CTCTTCGGTACAATCCGGCG+CGG - chr7.3:90835112-90835131 MS.gene80970:CDS 60.0%
CGCGCCGGATTGTACCGAAG+AGG + chr7.3:90835114-90835133 None:intergenic 65.0%
GGCGCGGTGGCTCCATTTGT+TGG - chr7.3:90835128-90835147 MS.gene80970:CDS 65.0%
TTCGGTACAATCCGGCGCGG+TGG - chr7.3:90835115-90835134 MS.gene80970:CDS 65.0%


Chromosome Type Strat End Strand Name
chr7.3 gene 90835062 90835881 90835062 ID=MS.gene80970
chr7.3 mRNA 90835062 90835881 90835062 ID=MS.gene80970.t1;Parent=MS.gene80970
chr7.3 exon 90835702 90835881 90835702 ID=MS.gene80970.t1.exon1;Parent=MS.gene80970.t1
chr7.3 CDS 90835702 90835881 90835702 ID=cds.MS.gene80970.t1;Parent=MS.gene80970.t1
chr7.3 exon 90835062 90835292 90835062 ID=MS.gene80970.t1.exon2;Parent=MS.gene80970.t1
chr7.3 CDS 90835062 90835292 90835062 ID=cds.MS.gene80970.t1;Parent=MS.gene80970.t1
Gene Sequence

>MS.gene80970

ATGGCTTCCTCTATAATTTCCTCTTCAGCTGTGACTACAGTTAACCGTGCCTCTTCGGTACAATCCGGCGCGGTGGCTCCATTTGTTGGACTCAAGTCCATGGCTGGCTTCCCAGTTACAAAGATCAACAAAGACATTACCTCCATTACAAGCAATGGTGGAAGAGTAAACTGCATGCAGGTGAGAAAATAATATACCCTACTATTTTAGTTTATTTGAGGCATAAGCACTTATGAGATTGTTTGATAGAGTTGTACAGATAAGCACTTATAGATTCGTTGGCAAAAGTACACAAGTTTGTTAACCATTTTGTGCAATTTTAGGTGTGGCCTCCTATTGGCAAGAAGAAGTTTGAGACTCTTTCTTTCATACCTTCCACCATTGACCAGAGAACAATTGGCTAAAGAAGTTGATTACCTTATAAGGAAGGGATGGGTTGCTTGCTTGGAATTTGAGACCGAGGTCTAATATTACTTTACTTGCAATATCTTCTCAATATATATAAACGTTGTTTTGTCGCGACCAAGGTCACAGTCACAAATTTGTATTTAACATATGTTTGTTTTGATTTGTACTTGGTTACTTGCAGAAAGGATTTGTGTACCGTGAGAACCACAAGTCACCAGGATACTATGATGGACGTTACTGGACAATGTGGAAGTTGCCTTTGTTTGGAGCAACCGATTCTTCTCAAGTGTTGAAGGAGCTTGATGAAGCTATTGCTGCTTACCCTGATGCCTTTGTCCGTATCATCGGATTCGACAACGTTCGTCAAGTTCAATGCATCAGTTTCATTGCACACACACCTGCAGTTTACTAA

Protein sequence

>MS.gene80970.t1

MASSIISSSAVTTVNRASSVQSGAVAPFVGLKSMAGFPVTKINKDITSITSNGGRVNCMQKGFVYRENHKSPGYYDGRYWTMWKLPLFGATDSSQVLKELDEAIAAYPDAFVRIIGFDNVRQVQCISFIAHTPAVY