AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene77928


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene77928.t1 MTR_3g030860 95.238 105 5 0 1 105 1 105 1.62e-58 175
MS.gene77928.t1 MTR_3g030850 81.905 105 18 1 1 104 1 105 2.82e-52 159
MS.gene77928.t1 MTR_4g037505 66.667 108 33 3 1 105 1 108 7.55e-39 125
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene77928.t1 AT1G51400 56.075 107 42 3 1 104 1 105 2.13e-28 99.4
MS.gene77928.t1 AT3G21055 55.140 107 42 4 1 105 1 103 2.86e-24 89.0
MS.gene77928.t1 AT3G21055 55.140 107 42 4 1 105 41 143 5.06e-24 89.4

Find 32 sgRNAs with CRISPR-Local

Find 36 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCTGCTGTTTGCTCTGTTGC+TGG 0.185357 3.2:-21581118 MS.gene77928:CDS
GCCTCTGGGGTGCCTCTTCT+TGG 0.187436 3.2:+21581073 None:intergenic
CTGCTGTTTGCTCTGTTGCT+GGG 0.300217 3.2:-21581117 MS.gene77928:CDS
ATTGCTACCCTCCTTGTCAT+TGG 0.354454 3.2:+21581174 None:intergenic
GTTGGGTTAGTGACACAAAC+AGG 0.359822 3.2:+21581034 None:intergenic
CTCTGTTGCTGGGGTTGCAA+TGG 0.391387 3.2:-21581107 MS.gene77928:CDS
AACCGATGATCGGTTGGTTA+CGG 0.418255 3.2:+21581264 None:intergenic
GATGATCGGTTGGTTACGGC+TGG 0.474152 3.2:+21581268 None:intergenic
GCTAGGATTTGTCGCTATTG+AGG 0.474783 3.2:-21581010 None:intergenic
TGCTGTTTGCTCTGTTGCTG+GGG 0.508878 3.2:-21581116 MS.gene77928:CDS
TAGCGACAAATCCTAGCAGT+TGG 0.523285 3.2:+21581016 None:intergenic
GCTGCGTTGCAACCGATGAT+CGG 0.526416 3.2:+21581254 None:intergenic
AGCGACAAATCCTAGCAGTT+GGG 0.546386 3.2:+21581017 None:intergenic
CGTTGCAACCGATGATCGGT+TGG 0.553263 3.2:+21581258 None:intergenic
AGTGCTGTAAGAGCAGTTGA+AGG 0.554404 3.2:-21581217 MS.gene77928:CDS
AATCACTATGACATCCTCAA+TGG 0.558898 3.2:-21581299 MS.gene77928:CDS
ACTATGACATCCTCAATGGT+TGG 0.565016 3.2:-21581295 MS.gene77928:CDS
GTGTCACTAACCCAACTGCT+AGG 0.567214 3.2:-21581027 MS.gene77928:CDS
AATGACAAGGAGGGTAGCAA+TGG 0.572603 3.2:-21581172 MS.gene77928:CDS
GTGCTGTAAGAGCAGTTGAA+GGG 0.585017 3.2:-21581216 MS.gene77928:CDS
AGCCGTAACCAACCGATCAT+CGG 0.587544 3.2:-21581266 MS.gene77928:CDS
ACAAGGAGGGTAGCAATGGA+AGG 0.594273 3.2:-21581168 MS.gene77928:CDS
TGCTGTAAGAGCAGTTGAAG+GGG 0.596756 3.2:-21581215 MS.gene77928:CDS
ATGATCGGTTGGTTACGGCT+GGG 0.625964 3.2:+21581269 None:intergenic
GGCTGGGAAACCAACCATTG+AGG 0.639229 3.2:+21581285 None:intergenic
GCAGATGATGAACCAAGAAG+AGG 0.660121 3.2:-21581085 MS.gene77928:CDS
GATCATCGGTTGCAACGCAG+CGG 0.682918 3.2:-21581252 MS.gene77928:CDS
AGTTGTACCAATGACAAGGA+GGG 0.684053 3.2:-21581181 MS.gene77928:CDS
CAGTTGTACCAATGACAAGG+AGG 0.687873 3.2:-21581182 MS.gene77928:CDS
TTGGGTTAGTGACACAAACA+GGG 0.691418 3.2:+21581035 None:intergenic
ACCAAGAAGAGGCACCCCAG+AGG 0.700077 3.2:-21581074 MS.gene77928:CDS
GATCAGTTGTACCAATGACA+AGG 0.701301 3.2:-21581185 MS.gene77928:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
AATCACTATGACATCCTCAA+TGG - chr3.2:21581020-21581039 MS.gene77928:CDS 35.0%
ACTATGACATCCTCAATGGT+TGG - chr3.2:21581024-21581043 MS.gene77928:CDS 40.0%
AGTTGTACCAATGACAAGGA+GGG - chr3.2:21581138-21581157 MS.gene77928:CDS 40.0%
GATCAGTTGTACCAATGACA+AGG - chr3.2:21581134-21581153 MS.gene77928:CDS 40.0%
!! TTGGGTTAGTGACACAAACA+GGG + chr3.2:21581287-21581306 None:intergenic 40.0%
!!! AACAGGGCCATACTTTTTCT+TGG + chr3.2:21581271-21581290 None:intergenic 40.0%
!!! ATACTTTTTCTTGGCCTCTG+GGG + chr3.2:21581262-21581281 None:intergenic 40.0%
!!! CATACTTTTTCTTGGCCTCT+GGG + chr3.2:21581263-21581282 None:intergenic 40.0%
AATGACAAGGAGGGTAGCAA+TGG - chr3.2:21581147-21581166 MS.gene77928:CDS 45.0%
AGCGACAAATCCTAGCAGTT+GGG + chr3.2:21581305-21581324 None:intergenic 45.0%
AGTGCTGTAAGAGCAGTTGA+AGG - chr3.2:21581102-21581121 MS.gene77928:CDS 45.0%
ATTGCTACCCTCCTTGTCAT+TGG + chr3.2:21581148-21581167 None:intergenic 45.0%
CAGTTGTACCAATGACAAGG+AGG - chr3.2:21581137-21581156 MS.gene77928:CDS 45.0%
CCAGAGGCCAAGAAAAAGTA+TGG - chr3.2:21581261-21581280 MS.gene77928:CDS 45.0%
GCAGATGATGAACCAAGAAG+AGG - chr3.2:21581234-21581253 MS.gene77928:CDS 45.0%
GTGCTGTAAGAGCAGTTGAA+GGG - chr3.2:21581103-21581122 MS.gene77928:CDS 45.0%
TAGCGACAAATCCTAGCAGT+TGG + chr3.2:21581306-21581325 None:intergenic 45.0%
TGCTGTAAGAGCAGTTGAAG+GGG - chr3.2:21581104-21581123 MS.gene77928:CDS 45.0%
! AACCGATGATCGGTTGGTTA+CGG + chr3.2:21581058-21581077 None:intergenic 45.0%
!! GTTGGGTTAGTGACACAAAC+AGG + chr3.2:21581288-21581307 None:intergenic 45.0%
!!! CCATACTTTTTCTTGGCCTC+TGG + chr3.2:21581264-21581283 None:intergenic 45.0%
ACAAGGAGGGTAGCAATGGA+AGG - chr3.2:21581151-21581170 MS.gene77928:CDS 50.0%
AGCCGTAACCAACCGATCAT+CGG - chr3.2:21581053-21581072 MS.gene77928:CDS 50.0%
! ATGATCGGTTGGTTACGGCT+GGG + chr3.2:21581053-21581072 None:intergenic 50.0%
! CTGCTGTTTGCTCTGTTGCT+GGG - chr3.2:21581202-21581221 MS.gene77928:CDS 50.0%
! GTGTCACTAACCCAACTGCT+AGG - chr3.2:21581292-21581311 MS.gene77928:CDS 50.0%
! TGCTGTTTGCTCTGTTGCTG+GGG - chr3.2:21581203-21581222 MS.gene77928:CDS 50.0%
CGTTGCAACCGATGATCGGT+TGG + chr3.2:21581064-21581083 None:intergenic 55.0%
CTCTGTTGCTGGGGTTGCAA+TGG - chr3.2:21581212-21581231 MS.gene77928:CDS 55.0%
GCTGCGTTGCAACCGATGAT+CGG + chr3.2:21581068-21581087 None:intergenic 55.0%
GGCTGGGAAACCAACCATTG+AGG + chr3.2:21581037-21581056 None:intergenic 55.0%
! GATCATCGGTTGCAACGCAG+CGG - chr3.2:21581067-21581086 MS.gene77928:CDS 55.0%
! GATGATCGGTTGGTTACGGC+TGG + chr3.2:21581054-21581073 None:intergenic 55.0%
! GCTGCTGTTTGCTCTGTTGC+TGG - chr3.2:21581201-21581220 MS.gene77928:CDS 55.0%
!! ACCAAGAAGAGGCACCCCAG+AGG - chr3.2:21581245-21581264 MS.gene77928:CDS 60.0%
!! GCCTCTGGGGTGCCTCTTCT+TGG + chr3.2:21581249-21581268 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr3.2 gene 21581012 21581329 21581012 ID=MS.gene77928
chr3.2 mRNA 21581012 21581329 21581012 ID=MS.gene77928.t1;Parent=MS.gene77928
chr3.2 exon 21581012 21581329 21581012 ID=MS.gene77928.t1.exon1;Parent=MS.gene77928.t1
chr3.2 CDS 21581012 21581329 21581012 ID=cds.MS.gene77928.t1;Parent=MS.gene77928.t1
Gene Sequence

>MS.gene77928

ATGGCATCAATCACTATGACATCCTCAATGGTTGGTTTCCCAGCCGTAACCAACCGATCATCGGTTGCAACGCAGCGGAGATTTGTTGTCAGTGCTGTAAGAGCAGTTGAAGGGGAAAAGACGATCAGTTGTACCAATGACAAGGAGGGTAGCAATGGAAGGAGAGATTTGATGTTTGCTGCAGCAGCAGCTGCTGTTTGCTCTGTTGCTGGGGTTGCAATGGCAGATGATGAACCAAGAAGAGGCACCCCAGAGGCCAAGAAAAAGTATGGCCCTGTTTGTGTCACTAACCCAACTGCTAGGATTTGTCGCTATTGA

Protein sequence

>MS.gene77928.t1

MASITMTSSMVGFPAVTNRSSVATQRRFVVSAVRAVEGEKTISCTNDKEGSNGRRDLMFAAAAAAVCSVAGVAMADDEPRRGTPEAKKKYGPVCVTNPTARICRY