AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene80968


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene80968.t1 MTR_7g007120 90.909 55 5 0 1 55 127 181 2.09e-33 112
MS.gene80968.t1 MTR_7g007120 89.091 55 6 0 1 55 127 181 8.88e-33 110
MS.gene80968.t1 MTR_7g007120 89.091 55 6 0 1 55 127 181 1.03e-32 110
MS.gene80968.t1 MTR_7g007200 89.091 55 6 0 1 55 127 181 1.05e-32 110
MS.gene80968.t1 MTR_7g007220 89.091 55 6 0 1 55 127 181 1.71e-32 109
MS.gene80968.t1 MTR_6g018300 89.091 55 6 0 1 55 123 177 4.09e-32 108
MS.gene80968.t1 MTR_6g018310 89.091 55 6 0 1 55 126 180 9.08e-32 107
MS.gene80968.t1 MTR_7g007230 87.273 55 7 0 1 55 127 181 1.29e-30 105
MS.gene80968.t1 MTR_7g007210 86.842 38 5 0 1 38 127 164 1.12e-19 77.4
MS.gene80968.t1 MTR_4g127870 50.000 52 26 0 1 52 116 167 2.70e-17 70.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene80968.t1 AT1G67090 70.909 55 16 0 1 55 124 178 3.44e-25 91.3
MS.gene80968.t1 AT5G38420 70.909 55 16 0 1 55 124 178 1.35e-24 89.7
MS.gene80968.t1 AT5G38430 70.909 55 16 0 1 55 124 178 1.46e-24 89.7
MS.gene80968.t1 AT5G38430 70.909 55 16 0 1 55 124 178 1.46e-24 89.7
MS.gene80968.t1 AT5G38410 70.909 55 16 0 1 55 129 183 1.56e-24 89.7
MS.gene80968.t1 AT5G38410 70.909 55 16 0 1 55 124 178 1.60e-24 89.7
MS.gene80968.t1 AT5G38410 70.909 55 16 0 1 55 117 171 1.62e-24 89.4

Find 10 sgRNAs with CRISPR-Local

Find 9 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGATTCTTCTCAAGTGTTGA+AGG 0.396980 7.3:-90811640 MS.gene80968:CDS
ATGTGGAAGTTGCCTTTGTT+TGG 0.411503 7.3:-90811669 MS.gene80968:CDS
ATGATACGGACAAAGGCATC+AGG 0.443523 7.3:+90811591 None:intergenic
CTTCAACACTTGAGAAGAAT+CGG 0.461194 7.3:+90811641 None:intergenic
GATGCCTTTGTCCGTATCAT+CGG 0.484184 7.3:-90811588 MS.gene80968:CDS
CGTTGTCGAATCCGATGATA+CGG 0.579522 7.3:+90811577 None:intergenic
ACTCAAACTTAGTAAACTGC+AGG 0.582384 7.3:+90811516 None:intergenic
GAATCGGTTGCTCCAAACAA+AGG 0.598394 7.3:+90811657 None:intergenic
TGATACGGACAAAGGCATCA+GGG 0.656050 7.3:+90811592 None:intergenic
GAATCCGATGATACGGACAA+AGG 0.687909 7.3:+90811584 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
CTTCAACACTTGAGAAGAAT+CGG + chr7.3:90811555-90811574 None:intergenic 35.0%
ATGTGGAAGTTGCCTTTGTT+TGG - chr7.3:90811524-90811543 MS.gene80968:CDS 40.0%
!! CGATTCTTCTCAAGTGTTGA+AGG - chr7.3:90811553-90811572 MS.gene80968:CDS 40.0%
CGTTGTCGAATCCGATGATA+CGG + chr7.3:90811619-90811638 None:intergenic 45.0%
GAATCCGATGATACGGACAA+AGG + chr7.3:90811612-90811631 None:intergenic 45.0%
GAATCGGTTGCTCCAAACAA+AGG + chr7.3:90811539-90811558 None:intergenic 45.0%
GATGCCTTTGTCCGTATCAT+CGG - chr7.3:90811605-90811624 MS.gene80968:CDS 45.0%
!! ATGATACGGACAAAGGCATC+AGG + chr7.3:90811605-90811624 None:intergenic 45.0%
!! TGATACGGACAAAGGCATCA+GGG + chr7.3:90811604-90811623 None:intergenic 45.0%


Chromosome Type Strat End Strand Name
chr7.3 gene 90811524 90811691 90811524 ID=MS.gene80968
chr7.3 mRNA 90811524 90811691 90811524 ID=MS.gene80968.t1;Parent=MS.gene80968
chr7.3 exon 90811524 90811691 90811524 ID=MS.gene80968.t1.exon1;Parent=MS.gene80968.t1
chr7.3 CDS 90811524 90811691 90811524 ID=cds.MS.gene80968.t1;Parent=MS.gene80968.t1
Gene Sequence

>MS.gene80968

ATGTGGAAGTTGCCTTTGTTTGGAGCAACCGATTCTTCTCAAGTGTTGAAGGAGCTTGATGAAGCTATTGCTGCTTACCCTGATGCCTTTGTCCGTATCATCGGATTCGACAACGTTCGTCAAGTTCAATGCATCAGTTTCATTGCACACACACCTGCAGTTTACTAA

Protein sequence

>MS.gene80968.t1

MWKLPLFGATDSSQVLKELDEAIAAYPDAFVRIIGFDNVRQVQCISFIAHTPAVY