Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene47784.t1 | ACJ83874.1 | 79.7 | 202 | 6 | 1 | 1 | 202 | 1 | 167 | 1.10E-71 | 279.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene47784.t1 | B7FGJ5 | 79.7 | 202 | 6 | 1 | 1 | 202 | 1 | 167 | 8.1e-72 | 279.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049171 | MS.gene47784 | 0.81967 | 1.03E-52 | -1.69E-46 |
| MS.gene049217 | MS.gene47784 | 0.83071 | 2.52E-55 | -1.69E-46 |
| MS.gene049222 | MS.gene47784 | 0.834931 | 2.25E-56 | -1.69E-46 |
| MS.gene049232 | MS.gene47784 | 0.809099 | 2.24E-50 | -1.69E-46 |
| MS.gene049285 | MS.gene47784 | 0.860549 | 1.91E-63 | -1.69E-46 |
| MS.gene049320 | MS.gene47784 | 0.85795 | 1.15E-62 | -1.69E-46 |
| MS.gene049369 | MS.gene47784 | 0.807969 | 3.90E-50 | -1.69E-46 |
| MS.gene049427 | MS.gene47784 | 0.802335 | 5.89E-49 | -1.69E-46 |
| MS.gene049609 | MS.gene47784 | 0.807918 | 4.00E-50 | -1.69E-46 |
| MS.gene049633 | MS.gene47784 | 0.83539 | 1.73E-56 | -1.69E-46 |
| MS.gene049805 | MS.gene47784 | 0.832794 | 7.71E-56 | -1.69E-46 |
| MS.gene05010 | MS.gene47784 | 0.822579 | 2.20E-53 | -1.69E-46 |
| MS.gene050163 | MS.gene47784 | 0.841781 | 3.86E-58 | -1.69E-46 |
| MS.gene050165 | MS.gene47784 | 0.836282 | 1.03E-56 | -1.69E-46 |
| MS.gene050323 | MS.gene47784 | 0.810217 | 1.29E-50 | -1.69E-46 |
| MS.gene050333 | MS.gene47784 | 0.862691 | 4.23E-64 | -1.69E-46 |
| MS.gene050432 | MS.gene47784 | 0.801138 | 1.04E-48 | -1.69E-46 |
| MS.gene050444 | MS.gene47784 | 0.829393 | 5.28E-55 | -1.69E-46 |
| MS.gene050504 | MS.gene47784 | 0.833439 | 5.33E-56 | -1.69E-46 |
| MS.gene050518 | MS.gene47784 | 0.842366 | 2.70E-58 | -1.69E-46 |
| MS.gene050593 | MS.gene47784 | 0.820017 | 8.57E-53 | -1.69E-46 |
| MS.gene050612 | MS.gene47784 | 0.861277 | 1.15E-63 | -1.69E-46 |
| MS.gene050662 | MS.gene47784 | 0.801573 | 8.45E-49 | -1.69E-46 |
| MS.gene050675 | MS.gene47784 | 0.847787 | 9.27E-60 | -1.69E-46 |
| MS.gene050699 | MS.gene47784 | 0.82599 | 3.47E-54 | -1.69E-46 |
| MS.gene050747 | MS.gene47784 | 0.806348 | 8.59E-50 | -1.69E-46 |
| MS.gene050767 | MS.gene47784 | 0.820705 | 5.96E-53 | -1.69E-46 |
| MS.gene050770 | MS.gene47784 | 0.822023 | 2.96E-53 | -1.69E-46 |
| MS.gene050772 | MS.gene47784 | 0.862633 | 4.41E-64 | -1.69E-46 |
| MS.gene05079 | MS.gene47784 | -0.803539 | 3.32E-49 | -1.69E-46 |
| MS.gene05080 | MS.gene47784 | 0.834795 | 2.44E-56 | -1.69E-46 |
| MS.gene050868 | MS.gene47784 | 0.818849 | 1.58E-52 | -1.69E-46 |
| MS.gene050871 | MS.gene47784 | 0.816953 | 4.24E-52 | -1.69E-46 |
| MS.gene050950 | MS.gene47784 | 0.810238 | 1.27E-50 | -1.69E-46 |
| MS.gene050986 | MS.gene47784 | 0.842713 | 2.19E-58 | -1.69E-46 |
| MS.gene051228 | MS.gene47784 | 0.894237 | 2.98E-75 | -1.69E-46 |
| MS.gene051317 | MS.gene47784 | 0.803798 | 2.94E-49 | -1.69E-46 |
| MS.gene051617 | MS.gene47784 | 0.81605 | 6.75E-52 | -1.69E-46 |
| MS.gene051810 | MS.gene47784 | 0.85145 | 8.81E-61 | -1.69E-46 |
| MS.gene051949 | MS.gene47784 | 0.81176 | 5.96E-51 | -1.69E-46 |
| MS.gene052271 | MS.gene47784 | 0.845265 | 4.52E-59 | -1.69E-46 |
| MS.gene052379 | MS.gene47784 | 0.855331 | 6.78E-62 | -1.69E-46 |
| MS.gene052615 | MS.gene47784 | 0.807268 | 5.49E-50 | -1.69E-46 |
| MS.gene052822 | MS.gene47784 | 0.80827 | 3.36E-50 | -1.69E-46 |
| MS.gene052870 | MS.gene47784 | 0.839015 | 2.04E-57 | -1.69E-46 |
| MS.gene052872 | MS.gene47784 | 0.843008 | 1.82E-58 | -1.69E-46 |
| MS.gene052916 | MS.gene47784 | 0.817396 | 3.37E-52 | -1.69E-46 |
| MS.gene052966 | MS.gene47784 | 0.81737 | 3.42E-52 | -1.69E-46 |
| MS.gene053009 | MS.gene47784 | 0.800403 | 1.47E-48 | -1.69E-46 |
| MS.gene053103 | MS.gene47784 | 0.811758 | 5.97E-51 | -1.69E-46 |
| MS.gene053104 | MS.gene47784 | 0.854797 | 9.69E-62 | -1.69E-46 |
| MS.gene053207 | MS.gene47784 | 0.819247 | 1.28E-52 | -1.69E-46 |
| MS.gene053213 | MS.gene47784 | 0.846436 | 2.17E-59 | -1.69E-46 |
| MS.gene053286 | MS.gene47784 | 0.84405 | 9.61E-59 | -1.69E-46 |
| MS.gene053322 | MS.gene47784 | 0.840083 | 1.08E-57 | -1.69E-46 |
| MS.gene053384 | MS.gene47784 | 0.823983 | 1.03E-53 | -1.69E-46 |
| MS.gene053449 | MS.gene47784 | 0.806452 | 8.17E-50 | -1.69E-46 |
| MS.gene053479 | MS.gene47784 | 0.81028 | 1.25E-50 | -1.69E-46 |
| MS.gene053574 | MS.gene47784 | 0.815902 | 7.29E-52 | -1.69E-46 |
| MS.gene053632 | MS.gene47784 | 0.821633 | 3.64E-53 | -1.69E-46 |
| MS.gene053824 | MS.gene47784 | 0.828726 | 7.66E-55 | -1.69E-46 |
| MS.gene053916 | MS.gene47784 | 0.859415 | 4.20E-63 | -1.69E-46 |
| MS.gene054543 | MS.gene47784 | 0.822192 | 2.70E-53 | -1.69E-46 |
| MS.gene054595 | MS.gene47784 | 0.809641 | 1.71E-50 | -1.69E-46 |
| MS.gene054600 | MS.gene47784 | 0.827784 | 1.29E-54 | -1.69E-46 |
| MS.gene054642 | MS.gene47784 | 0.877234 | 7.40E-69 | -1.69E-46 |
| MS.gene054954 | MS.gene47784 | 0.835634 | 1.50E-56 | -1.69E-46 |
| MS.gene05505 | MS.gene47784 | 0.822478 | 2.32E-53 | -1.69E-46 |
| MS.gene055206 | MS.gene47784 | 0.823972 | 1.04E-53 | -1.69E-46 |
| MS.gene055212 | MS.gene47784 | 0.808499 | 3.01E-50 | -1.69E-46 |
| MS.gene055224 | MS.gene47784 | 0.824439 | 8.07E-54 | -1.69E-46 |
| MS.gene055362 | MS.gene47784 | 0.834397 | 3.07E-56 | -1.69E-46 |
| MS.gene055397 | MS.gene47784 | 0.819001 | 1.46E-52 | -1.69E-46 |
| MS.gene055499 | MS.gene47784 | 0.802394 | 5.73E-49 | -1.69E-46 |
| MS.gene055502 | MS.gene47784 | 0.847635 | 1.02E-59 | -1.69E-46 |
| MS.gene055577 | MS.gene47784 | 0.820689 | 6.01E-53 | -1.69E-46 |
| MS.gene055636 | MS.gene47784 | 0.87301 | 2.05E-67 | -1.69E-46 |
| MS.gene055638 | MS.gene47784 | 0.853065 | 3.06E-61 | -1.69E-46 |
| MS.gene055647 | MS.gene47784 | 0.848029 | 7.95E-60 | -1.69E-46 |
| MS.gene055722 | MS.gene47784 | 0.845196 | 4.72E-59 | -1.69E-46 |
| MS.gene055748 | MS.gene47784 | 0.810312 | 1.23E-50 | -1.69E-46 |
| MS.gene055750 | MS.gene47784 | 0.808051 | 3.74E-50 | -1.69E-46 |
| MS.gene055751 | MS.gene47784 | 0.815321 | 9.82E-52 | -1.69E-46 |
| MS.gene05592 | MS.gene47784 | 0.889185 | 3.04E-73 | -1.69E-46 |
| MS.gene055941 | MS.gene47784 | 0.84851 | 5.85E-60 | -1.69E-46 |
| MS.gene055956 | MS.gene47784 | -0.826012 | 3.43E-54 | -1.69E-46 |
| MS.gene056374 | MS.gene47784 | 0.824315 | 8.63E-54 | -1.69E-46 |
| MS.gene056422 | MS.gene47784 | 0.803744 | 3.01E-49 | -1.69E-46 |
| MS.gene056487 | MS.gene47784 | 0.800769 | 1.23E-48 | -1.69E-46 |
| MS.gene056504 | MS.gene47784 | 0.808055 | 3.74E-50 | -1.69E-46 |
| MS.gene056510 | MS.gene47784 | 0.847999 | 8.11E-60 | -1.69E-46 |
| MS.gene056609 | MS.gene47784 | 0.805915 | 1.06E-49 | -1.69E-46 |
| MS.gene056657 | MS.gene47784 | 0.858137 | 1.01E-62 | -1.69E-46 |
| MS.gene056897 | MS.gene47784 | 0.814003 | 1.92E-51 | -1.69E-46 |
| MS.gene056898 | MS.gene47784 | 0.817575 | 3.07E-52 | -1.69E-46 |
| MS.gene056947 | MS.gene47784 | 0.800637 | 1.31E-48 | -1.69E-46 |
| MS.gene057011 | MS.gene47784 | 0.811614 | 6.41E-51 | -1.69E-46 |
| MS.gene057076 | MS.gene47784 | 0.835733 | 1.41E-56 | -1.69E-46 |
| MS.gene057129 | MS.gene47784 | 0.846658 | 1.89E-59 | -1.69E-46 |
| MS.gene057246 | MS.gene47784 | 0.81011 | 1.36E-50 | -1.69E-46 |
| MS.gene057331 | MS.gene47784 | 0.852916 | 3.37E-61 | -1.69E-46 |
| MS.gene05735 | MS.gene47784 | 0.818723 | 1.69E-52 | -1.69E-46 |
| MS.gene057416 | MS.gene47784 | 0.848271 | 6.82E-60 | -1.69E-46 |
| MS.gene057926 | MS.gene47784 | 0.831733 | 1.41E-55 | -1.69E-46 |
| MS.gene058323 | MS.gene47784 | 0.829182 | 5.94E-55 | -1.69E-46 |
| MS.gene05837 | MS.gene47784 | 0.812669 | 3.77E-51 | -1.69E-46 |
| MS.gene058419 | MS.gene47784 | 0.85351 | 2.28E-61 | -1.69E-46 |
| MS.gene058459 | MS.gene47784 | 0.806505 | 7.96E-50 | -1.69E-46 |
| MS.gene058544 | MS.gene47784 | 0.809098 | 2.24E-50 | -1.69E-46 |
| MS.gene058588 | MS.gene47784 | 0.801399 | 9.17E-49 | -1.69E-46 |
| MS.gene058723 | MS.gene47784 | 0.815786 | 7.74E-52 | -1.69E-46 |
| MS.gene058773 | MS.gene47784 | 0.859094 | 5.24E-63 | -1.69E-46 |
| MS.gene059022 | MS.gene47784 | 0.82947 | 5.06E-55 | -1.69E-46 |
| MS.gene059023 | MS.gene47784 | 0.808568 | 2.91E-50 | -1.69E-46 |
| MS.gene059029 | MS.gene47784 | 0.842744 | 2.14E-58 | -1.69E-46 |
| MS.gene059079 | MS.gene47784 | 0.802017 | 6.85E-49 | -1.69E-46 |
| MS.gene05913 | MS.gene47784 | 0.83965 | 1.40E-57 | -1.69E-46 |
| MS.gene059305 | MS.gene47784 | 0.841653 | 4.17E-58 | -1.69E-46 |
| MS.gene05934 | MS.gene47784 | 0.805817 | 1.11E-49 | -1.69E-46 |
| MS.gene059677 | MS.gene47784 | 0.839283 | 1.74E-57 | -1.69E-46 |
| MS.gene059711 | MS.gene47784 | 0.812069 | 5.10E-51 | -1.69E-46 |
| MS.gene059718 | MS.gene47784 | 0.827913 | 1.20E-54 | -1.69E-46 |
| MS.gene059865 | MS.gene47784 | 0.826662 | 2.40E-54 | -1.69E-46 |
| MS.gene059960 | MS.gene47784 | 0.839549 | 1.48E-57 | -1.69E-46 |
| MS.gene060762 | MS.gene47784 | 0.832186 | 1.09E-55 | -1.69E-46 |
| MS.gene060804 | MS.gene47784 | 0.82373 | 1.18E-53 | -1.69E-46 |
| MS.gene060818 | MS.gene47784 | 0.863706 | 2.05E-64 | -1.69E-46 |
| MS.gene060835 | MS.gene47784 | 0.817396 | 3.37E-52 | -1.69E-46 |
| MS.gene06092 | MS.gene47784 | 0.821643 | 3.62E-53 | -1.69E-46 |
| MS.gene060982 | MS.gene47784 | 0.810673 | 1.03E-50 | -1.69E-46 |
| MS.gene061041 | MS.gene47784 | 0.824743 | 6.84E-54 | -1.69E-46 |
| MS.gene061134 | MS.gene47784 | 0.835158 | 1.97E-56 | -1.69E-46 |
| MS.gene061150 | MS.gene47784 | 0.820798 | 5.67E-53 | -1.69E-46 |
| MS.gene061200 | MS.gene47784 | 0.838478 | 2.81E-57 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene47784.t1 | MTR_7g056413 | 94.608 | 204 | 9 | 1 | 1 | 202 | 1 | 204 | 1.23e-88 | 259 |
| MS.gene47784.t1 | MTR_2g026670 | 43.254 | 252 | 93 | 7 | 1 | 203 | 1 | 251 | 9.47e-45 | 149 |
| MS.gene47784.t1 | MTR_4g133900 | 80.556 | 72 | 14 | 0 | 4 | 75 | 8 | 79 | 1.25e-32 | 118 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene47784.t1 | AT4G16380 | 66.667 | 75 | 25 | 0 | 2 | 76 | 13 | 87 | 3.42e-30 | 112 |
| MS.gene47784.t1 | AT4G16380 | 66.667 | 75 | 25 | 0 | 2 | 76 | 6 | 80 | 4.03e-30 | 111 |
| MS.gene47784.t1 | AT1G51090 | 36.453 | 203 | 97 | 5 | 1 | 203 | 1 | 171 | 1.46e-24 | 95.1 |
| MS.gene47784.t1 | AT1G49420 | 50.633 | 79 | 38 | 1 | 2 | 79 | 6 | 84 | 5.48e-19 | 81.3 |
| MS.gene47784.t1 | AT4G16380 | 64.444 | 45 | 16 | 0 | 32 | 76 | 15 | 59 | 2.40e-14 | 69.7 |
Find 70 sgRNAs with CRISPR-Local
Find 96 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTTCCTTATGGGCATGTTTA+TGG | 0.209138 | 7.4:-47185052 | MS.gene47784:CDS |
| CCTTCGCCAGTTGTTCCTTA+TGG | 0.209497 | 7.4:-47185064 | MS.gene47784:CDS |
| GAGACAATATTTGTTGTAAT+GGG | 0.242382 | 7.4:-47185333 | MS.gene47784:CDS |
| CTTCGCCAGTTGTTCCTTAT+GGG | 0.271859 | 7.4:-47185063 | MS.gene47784:CDS |
| AACTATTTCTATGCTCTTGA+TGG | 0.278008 | 7.4:+47185303 | None:intergenic |
| CACAAGGACCCTTCAAATTA+TGG | 0.285976 | 7.4:+47184904 | None:intergenic |
| ATATTGTCTCTTACCTTCTC+TGG | 0.310383 | 7.4:+47185346 | None:intergenic |
| TCAGGTGCTGGCTTAGGCTT+TGG | 0.327997 | 7.4:+47185274 | None:intergenic |
| TCTTGCTCTTTGGGCTTCTC+AGG | 0.333827 | 7.4:+47185256 | None:intergenic |
| GCTGGAGCTGGTGCCGGAGC+TGG | 0.339691 | 7.4:+47185163 | None:intergenic |
| ATCGGCTTTGCCTCGGCCAC+GGG | 0.356925 | 7.4:+47185226 | None:intergenic |
| GCTGGTGCCGGAGCTGGAGC+TGG | 0.357949 | 7.4:+47185169 | None:intergenic |
| CCATCCATAACTATCGTATA+CGG | 0.371058 | 7.4:+47184991 | None:intergenic |
| AGAGACAATATTTGTTGTAA+TGG | 0.372434 | 7.4:-47185334 | MS.gene47784:CDS |
| GGCGGGCCAGGTTGTGCAAT+CGG | 0.376262 | 7.4:+47185208 | None:intergenic |
| GCCGGAGCTGGAGCTGGAGC+TGG | 0.377980 | 7.4:+47185175 | None:intergenic |
| GCTGCAGCACACCACTCTTA+TGG | 0.386416 | 7.4:+47185369 | None:intergenic |
| TGGGTCATCACCACGATTGC+TGG | 0.388653 | 7.4:-47184952 | MS.gene47784:CDS |
| TCTTTGGGCTTCTCAGGTGC+TGG | 0.389973 | 7.4:+47185262 | None:intergenic |
| AATCGGCTTTGCCTCGGCCA+CGG | 0.401999 | 7.4:+47185225 | None:intergenic |
| GAAGGAAGTGGAGCCGGAGC+TGG | 0.407891 | 7.4:+47185145 | None:intergenic |
| TATGGGCATGTTTATGGTGC+TGG | 0.421138 | 7.4:-47185046 | MS.gene47784:CDS |
| GGTGCAGCCGCAGAAGGAAG+TGG | 0.444486 | 7.4:+47185133 | None:intergenic |
| GCCGCAGAAGGAAGTGGAGC+CGG | 0.446508 | 7.4:+47185139 | None:intergenic |
| ACATGCCCATAAGGAACAAC+TGG | 0.446939 | 7.4:+47185058 | None:intergenic |
| GCCGGAGCTGGAGCTGGTGC+CGG | 0.450360 | 7.4:+47185157 | None:intergenic |
| GGTGCTGGCTTAGGCTTTGG+TGG | 0.453261 | 7.4:+47185277 | None:intergenic |
| TATTGTCTCTTACCTTCTCT+GGG | 0.459846 | 7.4:+47185347 | None:intergenic |
| CATGTTTATGGTGCTGGACA+AGG | 0.462627 | 7.4:-47185040 | MS.gene47784:CDS |
| GGCTTCTCAGGTGCTGGCTT+AGG | 0.470891 | 7.4:+47185268 | None:intergenic |
| GCACCATAAACATGCCCATA+AGG | 0.487789 | 7.4:+47185049 | None:intergenic |
| AGTGGAGCCGGAGCTGGAGC+TGG | 0.488934 | 7.4:+47185151 | None:intergenic |
| GTTACTAACCTTCTGCTCCA+TGG | 0.489005 | 7.4:+47185948 | None:intergenic |
| TTGTGCAATCGGCTTTGCCT+CGG | 0.490744 | 7.4:+47185219 | None:intergenic |
| TCCGGCACCAGCTCCAGCTC+CGG | 0.493234 | 7.4:-47185158 | MS.gene47784:CDS |
| CGACATGTACCATAATTTGA+AGG | 0.496633 | 7.4:-47184913 | None:intergenic |
| CTTCTTCGCGCCAGCAATCG+TGG | 0.498790 | 7.4:+47184942 | None:intergenic |
| GACAATATTTGTTGTAATGG+GGG | 0.502260 | 7.4:-47185331 | MS.gene47784:CDS |
| GCAAAGCCGATTGCACAACC+TGG | 0.511042 | 7.4:-47185214 | MS.gene47784:CDS |
| TCCAGCTCCAGCTCCAGCTC+CGG | 0.512738 | 7.4:-47185176 | MS.gene47784:CDS |
| CAAATATATGATGAGAAGAA+TGG | 0.515105 | 7.4:-47185400 | MS.gene47784:CDS |
| CGATTGCTGGCGCGAAGAAG+AGG | 0.521477 | 7.4:-47184939 | MS.gene47784:CDS |
| AGACAATATTTGTTGTAATG+GGG | 0.530751 | 7.4:-47185332 | MS.gene47784:CDS |
| AGGCCCGTATACGATAGTTA+TGG | 0.549421 | 7.4:-47184995 | MS.gene47784:CDS |
| GCCCTAAGCCATGGAGCAGA+AGG | 0.553044 | 7.4:-47185956 | None:intergenic |
| TGGTGATGACCCACGTAACA+AGG | 0.557049 | 7.4:+47184962 | None:intergenic |
| ACAGCTGGTGCAGCCGCAGA+AGG | 0.558520 | 7.4:+47185127 | None:intergenic |
| TGGCGAAGGATATGCAAGAA+TGG | 0.564765 | 7.4:+47185078 | None:intergenic |
| ATGGAGTGGGCCTTGTTACG+TGG | 0.565179 | 7.4:-47184972 | MS.gene47784:CDS |
| TACGATAGTTATGGATGGAG+TGG | 0.565922 | 7.4:-47184986 | MS.gene47784:CDS |
| TCCGGCTCCACTTCCTTCTG+CGG | 0.581939 | 7.4:-47185140 | MS.gene47784:CDS |
| ACGATAGTTATGGATGGAGT+GGG | 0.583338 | 7.4:-47184985 | MS.gene47784:CDS |
| GGCCTCCCATAAAACTGTGG+GGG | 0.585436 | 7.4:+47185013 | None:intergenic |
| TTATGCAGGTTGCTATAATG+AGG | 0.585675 | 7.4:-47185775 | MS.gene47784:intron |
| CATCCATAACTATCGTATAC+GGG | 0.586554 | 7.4:+47184992 | None:intergenic |
| GGTGTGCTGCAGCCCAGAGA+AGG | 0.588814 | 7.4:-47185359 | MS.gene47784:CDS |
| CGGGCCTCCCATAAAACTGT+GGG | 0.590176 | 7.4:+47185011 | None:intergenic |
| GTGTGAGGATACATTACAGC+TGG | 0.610753 | 7.4:+47185112 | None:intergenic |
| TGGAGTGGGCCTTGTTACGT+GGG | 0.611082 | 7.4:-47184971 | MS.gene47784:CDS |
| GCCTCCCATAAAACTGTGGG+GGG | 0.618515 | 7.4:+47185014 | None:intergenic |
| CCATAAGGAACAACTGGCGA+AGG | 0.625985 | 7.4:+47185064 | None:intergenic |
| GGTTGCTATAATGAGGCTCA+AGG | 0.630973 | 7.4:-47185768 | MS.gene47784:CDS |
| CCGTATACGATAGTTATGGA+TGG | 0.632117 | 7.4:-47184991 | MS.gene47784:CDS |
| ACTCACGAGGGTATTTGCAA+AGG | 0.638434 | 7.4:+47185702 | None:intergenic |
| GTTTATGGTGCTGGACAAGG+TGG | 0.639163 | 7.4:-47185037 | MS.gene47784:CDS |
| GGATATGCAAGAATGGACAT+TGG | 0.650120 | 7.4:+47185085 | None:intergenic |
| ATGGACATTGGCGTTGTGTG+AGG | 0.663282 | 7.4:+47185097 | None:intergenic |
| TGGTATAGTGACCATAAGAG+TGG | 0.664319 | 7.4:-47185380 | MS.gene47784:CDS |
| ACGGGCCTCCCATAAAACTG+TGG | 0.672573 | 7.4:+47185010 | None:intergenic |
| GGGCCTCCCATAAAACTGTG+GGG | 0.723372 | 7.4:+47185012 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAAAAAAATGAAATATGAG+AGG | + | chr7.4:47185206-47185225 | None:intergenic | 15.0% |
| !! | ATAATTAAGAAGCTAAAATT+AGG | + | chr7.4:47185357-47185376 | None:intergenic | 15.0% |
| !! | ATACAAAGTCTATATTTAAT+AGG | + | chr7.4:47185286-47185305 | None:intergenic | 15.0% |
| !! | GTTATTATACTATAACTAAT+TGG | + | chr7.4:47185016-47185035 | None:intergenic | 15.0% |
| !!! | ATTCAAGCATTTAATTTTAA+TGG | + | chr7.4:47185038-47185057 | None:intergenic | 15.0% |
| ! | CAAATATATGATGAGAAGAA+TGG | - | chr7.4:47185462-47185481 | MS.gene47784:intron | 25.0% |
| ! | GCAATACTAACAAATTAAGA+AGG | + | chr7.4:47185396-47185415 | None:intergenic | 25.0% |
| !! | GGTGGTGTTAATATATTATT+AGG | - | chr7.4:47185252-47185271 | MS.gene47784:CDS | 25.0% |
| !! | TTTTTCCCTTATGCATATTT+TGG | - | chr7.4:47185226-47185245 | MS.gene47784:CDS | 25.0% |
| !!! | AGACAATATTTGTTGTAATG+GGG | - | chr7.4:47185530-47185549 | MS.gene47784:intron | 25.0% |
| !!! | AGAGACAATATTTGTTGTAA+TGG | - | chr7.4:47185528-47185547 | MS.gene47784:intron | 25.0% |
| !!! | GAGACAATATTTGTTGTAAT+GGG | - | chr7.4:47185529-47185548 | MS.gene47784:intron | 25.0% |
| ACCATACCAAAATATGCATA+AGG | + | chr7.4:47185235-47185254 | None:intergenic | 30.0% | |
| CCATACCAAAATATGCATAA+GGG | + | chr7.4:47185234-47185253 | None:intergenic | 30.0% | |
| TATTATTGAAAGACTCACGA+GGG | + | chr7.4:47185175-47185194 | None:intergenic | 30.0% | |
| TTATTATTGAAAGACTCACG+AGG | + | chr7.4:47185176-47185195 | None:intergenic | 30.0% | |
| ! | AACTAATTGGCTTAGTTAAG+AGG | + | chr7.4:47185003-47185022 | None:intergenic | 30.0% |
| ! | TACAGCATTTTCTACATTGA+AGG | + | chr7.4:47185124-47185143 | None:intergenic | 30.0% |
| ! | TTCTTTTCTTGAACTTATGC+AGG | - | chr7.4:47185073-47185092 | MS.gene47784:CDS | 30.0% |
| !! | AACTATTTCTATGCTCTTGA+TGG | + | chr7.4:47185562-47185581 | None:intergenic | 30.0% |
| !! | GACAATATTTGTTGTAATGG+GGG | - | chr7.4:47185531-47185550 | MS.gene47784:intron | 30.0% |
| !!! | TTATGCATATTTTGGTATGG+TGG | - | chr7.4:47185234-47185253 | MS.gene47784:CDS | 30.0% |
| ATATTGTCTCTTACCTTCTC+TGG | + | chr7.4:47185519-47185538 | None:intergenic | 35.0% | |
| CATCCATAACTATCGTATAC+GGG | + | chr7.4:47185873-47185892 | None:intergenic | 35.0% | |
| CCATCCATAACTATCGTATA+CGG | + | chr7.4:47185874-47185893 | None:intergenic | 35.0% | |
| TATTGTCTCTTACCTTCTCT+GGG | + | chr7.4:47185518-47185537 | None:intergenic | 35.0% | |
| TTATGCAGGTTGCTATAATG+AGG | - | chr7.4:47185087-47185106 | MS.gene47784:CDS | 35.0% | |
| ! | ATTGGCTTAGTTAAGAGGAA+TGG | + | chr7.4:47184998-47185017 | None:intergenic | 35.0% |
| !! | AATATTTGTTGTAATGGGGG+TGG | - | chr7.4:47185534-47185553 | MS.gene47784:intron | 35.0% |
| !!! | CCCTTATGCATATTTTGGTA+TGG | - | chr7.4:47185231-47185250 | MS.gene47784:CDS | 35.0% |
| !!! | GGCTTTTTTTCTTGCTCTTT+GGG | + | chr7.4:47185618-47185637 | None:intergenic | 35.0% |
| ACGATAGTTATGGATGGAGT+GGG | - | chr7.4:47185877-47185896 | MS.gene47784:intron | 40.0% | |
| CCGTATACGATAGTTATGGA+TGG | - | chr7.4:47185871-47185890 | MS.gene47784:intron | 40.0% | |
| GGATATGCAAGAATGGACAT+TGG | + | chr7.4:47185780-47185799 | None:intergenic | 40.0% | |
| GTTCCTTATGGGCATGTTTA+TGG | - | chr7.4:47185810-47185829 | MS.gene47784:intron | 40.0% | |
| TACGATAGTTATGGATGGAG+TGG | - | chr7.4:47185876-47185895 | MS.gene47784:intron | 40.0% | |
| TGGTATAGTGACCATAAGAG+TGG | - | chr7.4:47185482-47185501 | MS.gene47784:intron | 40.0% | |
| !!! | GGGCTTTTTTTCTTGCTCTT+TGG | + | chr7.4:47185619-47185638 | None:intergenic | 40.0% |
| ACATGCCCATAAGGAACAAC+TGG | + | chr7.4:47185807-47185826 | None:intergenic | 45.0% | |
| AGAGCAAGAAAAAAAGCCCG+TGG | - | chr7.4:47185620-47185639 | MS.gene47784:intron | 45.0% | |
| AGGCCCGTATACGATAGTTA+TGG | - | chr7.4:47185867-47185886 | MS.gene47784:intron | 45.0% | |
| CTTCGCCAGTTGTTCCTTAT+GGG | - | chr7.4:47185799-47185818 | MS.gene47784:intron | 45.0% | |
| GCACCATAAACATGCCCATA+AGG | + | chr7.4:47185816-47185835 | None:intergenic | 45.0% | |
| GGTTGCTATAATGAGGCTCA+AGG | - | chr7.4:47185094-47185113 | MS.gene47784:CDS | 45.0% | |
| GTGTGAGGATACATTACAGC+TGG | + | chr7.4:47185753-47185772 | None:intergenic | 45.0% | |
| TATGGGCATGTTTATGGTGC+TGG | - | chr7.4:47185816-47185835 | MS.gene47784:intron | 45.0% | |
| TGGCGAAGGATATGCAAGAA+TGG | + | chr7.4:47185787-47185806 | None:intergenic | 45.0% | |
| ! | CATGTTTATGGTGCTGGACA+AGG | - | chr7.4:47185822-47185841 | MS.gene47784:intron | 45.0% |
| !! | ACTCACGAGGGTATTTGCAA+AGG | + | chr7.4:47185163-47185182 | None:intergenic | 45.0% |
| !! | GAGCTGGATTTTGTTTAGGC+GGG | + | chr7.4:47185674-47185693 | None:intergenic | 45.0% |
| !! | GCTGGAGCTGGATTTTGTTT+AGG | + | chr7.4:47185678-47185697 | None:intergenic | 45.0% |
| !! | GGAGCTGGATTTTGTTTAGG+CGG | + | chr7.4:47185675-47185694 | None:intergenic | 45.0% |
| ATGGACATTGGCGTTGTGTG+AGG | + | chr7.4:47185768-47185787 | None:intergenic | 50.0% | |
| CCATAAGGAACAACTGGCGA+AGG | + | chr7.4:47185801-47185820 | None:intergenic | 50.0% | |
| CCTTCGCCAGTTGTTCCTTA+TGG | - | chr7.4:47185798-47185817 | MS.gene47784:intron | 50.0% | |
| TCTTGCTCTTTGGGCTTCTC+AGG | + | chr7.4:47185609-47185628 | None:intergenic | 50.0% | |
| ! | GTTTATGGTGCTGGACAAGG+TGG | - | chr7.4:47185825-47185844 | MS.gene47784:intron | 50.0% |
| ! | TGGTGATGACCCACGTAACA+AGG | + | chr7.4:47185903-47185922 | None:intergenic | 50.0% |
| ! | TTGTGCAATCGGCTTTGCCT+CGG | + | chr7.4:47185646-47185665 | None:intergenic | 50.0% |
| ACGGGCCTCCCATAAAACTG+TGG | + | chr7.4:47185855-47185874 | None:intergenic | 55.0% | |
| AGAAAAAAAGCCCGTGGCCG+AGG | - | chr7.4:47185626-47185645 | MS.gene47784:intron | 55.0% | |
| CGGGCCTCCCATAAAACTGT+GGG | + | chr7.4:47185854-47185873 | None:intergenic | 55.0% | |
| GCAAAGCCGATTGCACAACC+TGG | - | chr7.4:47185648-47185667 | MS.gene47784:intron | 55.0% | |
| GCCTCCCATAAAACTGTGGG+GGG | + | chr7.4:47185851-47185870 | None:intergenic | 55.0% | |
| GCTGCAGCACACCACTCTTA+TGG | + | chr7.4:47185496-47185515 | None:intergenic | 55.0% | |
| GGCCTCCCATAAAACTGTGG+GGG | + | chr7.4:47185852-47185871 | None:intergenic | 55.0% | |
| GGGCCTCCCATAAAACTGTG+GGG | + | chr7.4:47185853-47185872 | None:intergenic | 55.0% | |
| TCTTTGGGCTTCTCAGGTGC+TGG | + | chr7.4:47185603-47185622 | None:intergenic | 55.0% | |
| TGGGTCATCACCACGATTGC+TGG | - | chr7.4:47185910-47185929 | MS.gene47784:intron | 55.0% | |
| !! | ATGGAGTGGGCCTTGTTACG+TGG | - | chr7.4:47185890-47185909 | MS.gene47784:intron | 55.0% |
| !! | GGATTTTGTTTAGGCGGGCC+AGG | + | chr7.4:47185669-47185688 | None:intergenic | 55.0% |
| !! | GTGGCCCCCCACAGTTTTAT+GGG | - | chr7.4:47185844-47185863 | MS.gene47784:intron | 55.0% |
| !! | TCAGGTGCTGGCTTAGGCTT+TGG | + | chr7.4:47185591-47185610 | None:intergenic | 55.0% |
| !! | TGGAGTGGGCCTTGTTACGT+GGG | - | chr7.4:47185891-47185910 | MS.gene47784:intron | 55.0% |
| CGATTGCTGGCGCGAAGAAG+AGG | - | chr7.4:47185923-47185942 | MS.gene47784:intron | 60.0% | |
| CTTCTTCGCGCCAGCAATCG+TGG | + | chr7.4:47185923-47185942 | None:intergenic | 60.0% | |
| TCCGGCTCCACTTCCTTCTG+CGG | - | chr7.4:47185722-47185741 | MS.gene47784:CDS | 60.0% | |
| ! | AATCGGCTTTGCCTCGGCCA+CGG | + | chr7.4:47185640-47185659 | None:intergenic | 60.0% |
| ! | GGTGGCCCCCCACAGTTTTA+TGG | - | chr7.4:47185843-47185862 | MS.gene47784:intron | 60.0% |
| !! | GCCCCCCACAGTTTTATGGG+AGG | - | chr7.4:47185847-47185866 | MS.gene47784:intron | 60.0% |
| !! | GGCTTCTCAGGTGCTGGCTT+AGG | + | chr7.4:47185597-47185616 | None:intergenic | 60.0% |
| !! | GGTGCTGGCTTAGGCTTTGG+TGG | + | chr7.4:47185588-47185607 | None:intergenic | 60.0% |
| ACAGCTGGTGCAGCCGCAGA+AGG | + | chr7.4:47185738-47185757 | None:intergenic | 65.0% | |
| GAAGGAAGTGGAGCCGGAGC+TGG | + | chr7.4:47185720-47185739 | None:intergenic | 65.0% | |
| GCCGCAGAAGGAAGTGGAGC+CGG | + | chr7.4:47185726-47185745 | None:intergenic | 65.0% | |
| GGCGGGCCAGGTTGTGCAAT+CGG | + | chr7.4:47185657-47185676 | None:intergenic | 65.0% | |
| GGTGCAGCCGCAGAAGGAAG+TGG | + | chr7.4:47185732-47185751 | None:intergenic | 65.0% | |
| GGTGTGCTGCAGCCCAGAGA+AGG | - | chr7.4:47185503-47185522 | MS.gene47784:intron | 65.0% | |
| TCCAGCTCCAGCTCCAGCTC+CGG | - | chr7.4:47185686-47185705 | MS.gene47784:intron | 65.0% | |
| ! | ATCGGCTTTGCCTCGGCCAC+GGG | + | chr7.4:47185639-47185658 | None:intergenic | 65.0% |
| AGTGGAGCCGGAGCTGGAGC+TGG | + | chr7.4:47185714-47185733 | None:intergenic | 70.0% | |
| TCCGGCACCAGCTCCAGCTC+CGG | - | chr7.4:47185704-47185723 | MS.gene47784:intron | 70.0% | |
| GCCGGAGCTGGAGCTGGAGC+TGG | + | chr7.4:47185690-47185709 | None:intergenic | 75.0% | |
| GCCGGAGCTGGAGCTGGTGC+CGG | + | chr7.4:47185708-47185727 | None:intergenic | 75.0% | |
| ! | GCTGGAGCTGGTGCCGGAGC+TGG | + | chr7.4:47185702-47185721 | None:intergenic | 75.0% |
| ! | GCTGGTGCCGGAGCTGGAGC+TGG | + | chr7.4:47185696-47185715 | None:intergenic | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.4 | gene | 47184916 | 47185968 | 47184916 | ID=MS.gene47784 |
| chr7.4 | mRNA | 47184916 | 47185968 | 47184916 | ID=MS.gene47784.t1;Parent=MS.gene47784 |
| chr7.4 | exon | 47185957 | 47185968 | 47185957 | ID=MS.gene47784.t1.exon1;Parent=MS.gene47784.t1 |
| chr7.4 | CDS | 47185957 | 47185968 | 47185957 | ID=cds.MS.gene47784.t1;Parent=MS.gene47784.t1 |
| chr7.4 | exon | 47185708 | 47185789 | 47185708 | ID=MS.gene47784.t1.exon2;Parent=MS.gene47784.t1 |
| chr7.4 | CDS | 47185708 | 47185789 | 47185708 | ID=cds.MS.gene47784.t1;Parent=MS.gene47784.t1 |
| chr7.4 | exon | 47184916 | 47185433 | 47184916 | ID=MS.gene47784.t1.exon3;Parent=MS.gene47784.t1 |
| chr7.4 | CDS | 47184916 | 47185433 | 47184916 | ID=cds.MS.gene47784.t1;Parent=MS.gene47784.t1 |
| Gene Sequence |
| Protein sequence |
