AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene95520


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene95520.t1 MTR_3g030860 94.286 105 6 0 1 105 1 105 2.04e-57 172
MS.gene95520.t1 MTR_3g030850 80.952 105 19 1 1 104 1 105 4.01e-51 156
MS.gene95520.t1 MTR_4g037505 65.741 108 34 3 1 105 1 108 1.12e-37 122
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene95520.t1 AT1G51400 55.140 107 43 3 1 104 1 105 2.25e-27 97.1
MS.gene95520.t1 AT3G21055 54.206 107 43 4 1 105 1 103 3.02e-23 86.3
MS.gene95520.t1 AT3G21055 54.206 107 43 4 1 105 41 143 4.64e-23 87.0

Find 33 sgRNAs with CRISPR-Local

Find 37 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCTGCTGTTTGCTCTGTTGC+TGG 0.185357 3.4:-24355636 MS.gene95520:CDS
GCCTCTGGGGTGCCTCTTCT+TGG 0.187280 3.4:+24355591 None:intergenic
CTGCTGTTTGCTCTGTTGCT+GGG 0.300217 3.4:-24355635 MS.gene95520:CDS
ATTGCTACCCTCCTTGTCAT+TGG 0.354454 3.4:+24355692 None:intergenic
GTTGGGTTAGTGACACAAAC+AGG 0.359822 3.4:+24355552 None:intergenic
CTCTGTTGCTGGGGTTGCAA+TGG 0.391387 3.4:-24355625 MS.gene95520:CDS
AACCGATGATCGGTTGGTTA+CGG 0.418255 3.4:+24355782 None:intergenic
GATGATCGGTTGGTTACGGC+TGG 0.474152 3.4:+24355786 None:intergenic
GCTAGGATTTGTCGCTATTG+AGG 0.474783 3.4:-24355528 None:intergenic
TGCTGTTTGCTCTGTTGCTG+GGG 0.508878 3.4:-24355634 MS.gene95520:CDS
TAGCGACAAATCCTAGCAGT+TGG 0.523285 3.4:+24355534 None:intergenic
GCTGCGTTGCAACCGATGAT+CGG 0.526416 3.4:+24355772 None:intergenic
GGGGTTGCAATGGCAGATGA+TGG 0.535455 3.4:-24355615 MS.gene95520:CDS
AGCGACAAATCCTAGCAGTT+GGG 0.546386 3.4:+24355535 None:intergenic
CGTTGCAACCGATGATCGGT+TGG 0.553263 3.4:+24355776 None:intergenic
AGTGCTGTAAGAGCAGTTGA+AGG 0.554404 3.4:-24355735 MS.gene95520:CDS
AATCACTATGACATCCTCAA+TGG 0.558898 3.4:-24355817 MS.gene95520:CDS
ACTATGACATCCTCAATGGT+TGG 0.565016 3.4:-24355813 MS.gene95520:CDS
GTGTCACTAACCCAACTGCT+AGG 0.567214 3.4:-24355545 MS.gene95520:CDS
AATGACAAGGAGGGTAGCAA+TGG 0.572603 3.4:-24355690 MS.gene95520:CDS
GTGCTGTAAGAGCAGTTGAA+GGG 0.585017 3.4:-24355734 MS.gene95520:CDS
AGCCGTAACCAACCGATCAT+CGG 0.587544 3.4:-24355784 MS.gene95520:CDS
ACAAGGAGGGTAGCAATGGA+AGG 0.594273 3.4:-24355686 MS.gene95520:CDS
TGCTGTAAGAGCAGTTGAAG+GGG 0.596756 3.4:-24355733 MS.gene95520:CDS
ATGATCGGTTGGTTACGGCT+GGG 0.625964 3.4:+24355787 None:intergenic
GCAGATGATGGACCAAGAAG+AGG 0.628210 3.4:-24355603 MS.gene95520:CDS
GGCTGGGAAACCAACCATTG+AGG 0.639229 3.4:+24355803 None:intergenic
ACCAAGAAGAGGCACCCCAG+AGG 0.672940 3.4:-24355592 MS.gene95520:CDS
GATCATCGGTTGCAACGCAG+CGG 0.682918 3.4:-24355770 MS.gene95520:CDS
AGTTGTACCAATGACAAGGA+GGG 0.684053 3.4:-24355699 MS.gene95520:CDS
CAGTTGTACCAATGACAAGG+AGG 0.687873 3.4:-24355700 MS.gene95520:CDS
TTGGGTTAGTGACACAAACA+GGG 0.691418 3.4:+24355553 None:intergenic
GATCAGTTGTACCAATGACA+AGG 0.701301 3.4:-24355703 MS.gene95520:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
AATCACTATGACATCCTCAA+TGG - chr3.4:24355538-24355557 MS.gene95520:CDS 35.0%
ACTATGACATCCTCAATGGT+TGG - chr3.4:24355542-24355561 MS.gene95520:CDS 40.0%
AGTTGTACCAATGACAAGGA+GGG - chr3.4:24355656-24355675 MS.gene95520:CDS 40.0%
GATCAGTTGTACCAATGACA+AGG - chr3.4:24355652-24355671 MS.gene95520:CDS 40.0%
!! TTGGGTTAGTGACACAAACA+GGG + chr3.4:24355805-24355824 None:intergenic 40.0%
!!! AACAGGGCCATACTTTTTCT+TGG + chr3.4:24355789-24355808 None:intergenic 40.0%
!!! ATACTTTTTCTTGGCCTCTG+GGG + chr3.4:24355780-24355799 None:intergenic 40.0%
!!! CATACTTTTTCTTGGCCTCT+GGG + chr3.4:24355781-24355800 None:intergenic 40.0%
AATGACAAGGAGGGTAGCAA+TGG - chr3.4:24355665-24355684 MS.gene95520:CDS 45.0%
AGCGACAAATCCTAGCAGTT+GGG + chr3.4:24355823-24355842 None:intergenic 45.0%
AGTGCTGTAAGAGCAGTTGA+AGG - chr3.4:24355620-24355639 MS.gene95520:CDS 45.0%
ATTGCTACCCTCCTTGTCAT+TGG + chr3.4:24355666-24355685 None:intergenic 45.0%
CAGTTGTACCAATGACAAGG+AGG - chr3.4:24355655-24355674 MS.gene95520:CDS 45.0%
CCAGAGGCCAAGAAAAAGTA+TGG - chr3.4:24355779-24355798 MS.gene95520:CDS 45.0%
GTGCTGTAAGAGCAGTTGAA+GGG - chr3.4:24355621-24355640 MS.gene95520:CDS 45.0%
TAGCGACAAATCCTAGCAGT+TGG + chr3.4:24355824-24355843 None:intergenic 45.0%
TGCTGTAAGAGCAGTTGAAG+GGG - chr3.4:24355622-24355641 MS.gene95520:CDS 45.0%
! AACCGATGATCGGTTGGTTA+CGG + chr3.4:24355576-24355595 None:intergenic 45.0%
!! GTTGGGTTAGTGACACAAAC+AGG + chr3.4:24355806-24355825 None:intergenic 45.0%
!!! CCATACTTTTTCTTGGCCTC+TGG + chr3.4:24355782-24355801 None:intergenic 45.0%
ACAAGGAGGGTAGCAATGGA+AGG - chr3.4:24355669-24355688 MS.gene95520:CDS 50.0%
AGCCGTAACCAACCGATCAT+CGG - chr3.4:24355571-24355590 MS.gene95520:CDS 50.0%
! ATGATCGGTTGGTTACGGCT+GGG + chr3.4:24355571-24355590 None:intergenic 50.0%
! CTGCTGTTTGCTCTGTTGCT+GGG - chr3.4:24355720-24355739 MS.gene95520:CDS 50.0%
! GTGTCACTAACCCAACTGCT+AGG - chr3.4:24355810-24355829 MS.gene95520:CDS 50.0%
! TGCTGTTTGCTCTGTTGCTG+GGG - chr3.4:24355721-24355740 MS.gene95520:CDS 50.0%
!! GCAGATGATGGACCAAGAAG+AGG - chr3.4:24355752-24355771 MS.gene95520:CDS 50.0%
CGTTGCAACCGATGATCGGT+TGG + chr3.4:24355582-24355601 None:intergenic 55.0%
CTCTGTTGCTGGGGTTGCAA+TGG - chr3.4:24355730-24355749 MS.gene95520:CDS 55.0%
GCTGCGTTGCAACCGATGAT+CGG + chr3.4:24355586-24355605 None:intergenic 55.0%
GGCTGGGAAACCAACCATTG+AGG + chr3.4:24355555-24355574 None:intergenic 55.0%
GGGGTTGCAATGGCAGATGA+TGG - chr3.4:24355740-24355759 MS.gene95520:CDS 55.0%
! GATCATCGGTTGCAACGCAG+CGG - chr3.4:24355585-24355604 MS.gene95520:CDS 55.0%
! GATGATCGGTTGGTTACGGC+TGG + chr3.4:24355572-24355591 None:intergenic 55.0%
! GCTGCTGTTTGCTCTGTTGC+TGG - chr3.4:24355719-24355738 MS.gene95520:CDS 55.0%
!! ACCAAGAAGAGGCACCCCAG+AGG - chr3.4:24355763-24355782 MS.gene95520:CDS 60.0%
!! GCCTCTGGGGTGCCTCTTCT+TGG + chr3.4:24355767-24355786 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr3.4 gene 24355530 24355847 24355530 ID=MS.gene95520
chr3.4 mRNA 24355530 24355847 24355530 ID=MS.gene95520.t1;Parent=MS.gene95520
chr3.4 exon 24355530 24355847 24355530 ID=MS.gene95520.t1.exon1;Parent=MS.gene95520.t1
chr3.4 CDS 24355530 24355847 24355530 ID=cds.MS.gene95520.t1;Parent=MS.gene95520.t1
Gene Sequence

>MS.gene95520

ATGGCATCAATCACTATGACATCCTCAATGGTTGGTTTCCCAGCCGTAACCAACCGATCATCGGTTGCAACGCAGCGGAGATTTGTTGTCAGTGCTGTAAGAGCAGTTGAAGGGGAAAAGACGATCAGTTGTACCAATGACAAGGAGGGTAGCAATGGAAGGAGAGATTTGATGTTTGCTGCAGCAGCAGCTGCTGTTTGCTCTGTTGCTGGGGTTGCAATGGCAGATGATGGACCAAGAAGAGGCACCCCAGAGGCCAAGAAAAAGTATGGCCCTGTTTGTGTCACTAACCCAACTGCTAGGATTTGTCGCTATTGA

Protein sequence

>MS.gene95520.t1

MASITMTSSMVGFPAVTNRSSVATQRRFVVSAVRAVEGEKTISCTNDKEGSNGRRDLMFAAAAAAVCSVAGVAMADDGPRRGTPEAKKKYGPVCVTNPTARICRY