AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene60296


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene60296.t1 MTR_1g012690 79.870 154 28 1 1 151 1 154 1.48e-72 214
MS.gene60296.t1 MTR_1g012700 73.780 164 30 1 1 151 1 164 2.56e-72 214
MS.gene60296.t1 MTR_1g012710 96.026 151 6 0 1 151 1 151 8.87e-69 205
MS.gene60296.t1 MTR_1g012630 54.630 216 27 4 1 151 1 210 3.01e-55 172
MS.gene60296.t1 MTR_3g106740 61.589 151 35 3 1 150 1 129 2.31e-46 147
MS.gene60296.t1 MTR_4g101330 46.429 168 72 3 1 150 1 168 1.29e-37 126
MS.gene60296.t1 MTR_3g106750 57.792 154 37 3 1 151 1 129 6.31e-36 121
MS.gene60296.t1 MTR_4g108150 55.556 108 40 2 47 151 21 123 2.06e-34 117
MS.gene60296.t1 MTR_4g101260 63.855 83 30 0 69 151 50 132 4.64e-33 114
MS.gene60296.t1 MTR_4g101280 63.636 88 31 1 65 151 56 143 6.04e-32 111
MS.gene60296.t1 MTR_4g101310 59.091 88 35 1 65 151 47 134 2.61e-31 109
MS.gene60296.t1 MTR_2g026760 55.556 90 39 1 62 151 39 127 8.60e-27 97.8
MS.gene60296.t1 MTR_5g012340 50.575 87 41 2 65 150 91 176 1.40e-25 96.3
MS.gene60296.t1 MTR_5g012380 49.425 87 42 2 65 150 84 169 4.72e-25 94.7
MS.gene60296.t1 MTR_4g101270 52.941 85 38 2 67 150 103 186 2.39e-23 90.9
MS.gene60296.t1 MTR_2g026930 44.706 85 43 2 67 151 119 199 8.93e-20 81.6
MS.gene60296.t1 MTR_2g026920 44.706 85 43 2 67 151 115 195 1.90e-19 80.9
MS.gene60296.t1 MTR_3g082100 52.326 86 39 2 66 150 251 335 1.21e-17 78.2
MS.gene60296.t1 MTR_2g026775 44.872 78 38 2 76 151 686 760 3.39e-17 77.8
MS.gene60296.t1 MTR_4g115360 53.571 84 37 2 69 151 151 233 4.42e-13 64.7
MS.gene60296.t1 MTR_2g026820 50.000 70 31 2 74 142 542 608 6.35e-13 65.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene60296.t1 AT4G12490 45.055 182 69 3 1 151 1 182 4.62e-42 138
MS.gene60296.t1 AT2G45180 75.904 83 20 0 69 151 52 134 1.95e-39 130
MS.gene60296.t1 AT4G12470 66.292 89 29 1 64 151 73 161 1.50e-36 123
MS.gene60296.t1 AT4G12480 67.045 88 28 1 65 151 81 168 1.10e-35 121
MS.gene60296.t1 AT4G12500 64.773 88 30 1 65 151 90 177 5.99e-35 120
MS.gene60296.t1 AT1G62510 66.667 84 27 1 68 150 66 149 5.23e-33 114
MS.gene60296.t1 AT4G00165 49.533 107 48 2 45 150 27 128 3.12e-31 109
MS.gene60296.t1 AT4G00165 49.533 107 48 2 45 150 27 128 3.12e-31 109
MS.gene60296.t1 AT4G12520 47.059 153 55 4 1 151 1 129 1.16e-30 107
MS.gene60296.t1 AT4G12510 47.059 153 55 4 1 151 1 129 1.16e-30 107
MS.gene60296.t1 AT1G12100 43.151 146 60 4 7 151 9 132 1.47e-30 107
MS.gene60296.t1 AT5G46890 61.957 92 32 2 60 151 39 127 3.21e-30 106
MS.gene60296.t1 AT1G12090 65.000 80 27 1 72 150 58 137 1.42e-29 105
MS.gene60296.t1 AT5G46900 66.250 80 25 1 72 151 50 127 4.04e-29 103
MS.gene60296.t1 AT3G22142 56.701 97 39 3 55 150 1384 1478 2.66e-27 106
MS.gene60296.t1 AT4G12550 49.451 91 45 1 62 151 21 111 6.15e-27 97.8
MS.gene60296.t1 AT4G22460 47.059 119 44 2 32 149 31 131 3.08e-26 96.7
MS.gene60296.t1 AT4G12530 50.588 85 42 0 66 150 31 115 1.08e-25 94.7
MS.gene60296.t1 AT4G12545 50.549 91 41 2 62 151 21 108 1.54e-24 91.7
MS.gene60296.t1 AT2G10940 57.143 84 34 2 69 151 208 290 3.58e-23 92.4
MS.gene60296.t1 AT2G10940 57.143 84 34 2 69 151 208 290 3.58e-23 92.4
MS.gene60296.t1 AT3G22120 56.977 86 34 3 67 150 265 349 3.19e-21 88.2
MS.gene60296.t1 AT3G22120 56.977 86 34 3 67 150 248 332 5.12e-21 87.4
MS.gene60296.t1 AT4G15160 52.326 86 39 2 67 150 97 182 2.90e-19 80.1
MS.gene60296.t1 AT4G15160 52.326 86 39 2 67 150 179 264 4.98e-19 81.3
MS.gene60296.t1 AT1G62500 48.193 83 41 2 69 150 212 293 8.39e-19 80.9
MS.gene60296.t1 AT4G22490 38.710 93 50 3 61 150 28 116 3.16e-15 67.8

Find 34 sgRNAs with CRISPR-Local

Find 39 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGAGAGAAAATAATATAATT+AGG 0.201862 1.1:-8398595 None:intergenic
TTGCCTTTGTACTGCTATTA+AGG 0.221337 1.1:+8398907 MS.gene60296:CDS
GCTATTAAGGCTAATGTTCT+TGG 0.285703 1.1:+8398920 MS.gene60296:CDS
GCTTCAAGGTTTGGTTGATT+TGG 0.319262 1.1:+8398874 MS.gene60296:CDS
CTAGGCTTTGGCTTTGGCTT+AGG 0.338065 1.1:-8398677 None:intergenic
TTTGGCTTTGGCTTAGGCTT+AGG 0.367383 1.1:-8398671 None:intergenic
GGAGGACTAGGCTTTGGCTT+TGG 0.385876 1.1:-8398683 None:intergenic
GCTGGTGGAGGACTAGGCTT+TGG 0.388141 1.1:-8398689 None:intergenic
GGTGTGTGTGCTGATGTTCT+AGG 0.388503 1.1:+8398800 MS.gene60296:CDS
GAGAGAAAATAATATAATTA+GGG 0.389348 1.1:-8398594 None:intergenic
GGACTTGTTAATATTATTGT+TGG 0.394838 1.1:+8398821 MS.gene60296:CDS
AAGTGTTGTGCATTGCTTCA+AGG 0.409572 1.1:+8398860 MS.gene60296:CDS
TTGTGCATTGCTTCAAGGTT+TGG 0.410333 1.1:+8398865 MS.gene60296:CDS
AATTAGGGCACTAAGCTTGT+TGG 0.421182 1.1:-8398579 None:intergenic
GGTGAAGCTGGTGGAGGACT+AGG 0.427892 1.1:-8398695 None:intergenic
GGACATGGTGTTGGTGAAGC+TGG 0.438461 1.1:-8398707 None:intergenic
AGTAGGAGACTGAGTGTGAT+AGG 0.453246 1.1:-8398959 None:intergenic
GGAGGTGTTGCTGAAGGTGT+TGG 0.497717 1.1:-8398731 None:intergenic
GCTGAAGGTGTTGGAGGACA+TGG 0.512621 1.1:-8398722 None:intergenic
ACAAGAAAGTCTATGAACAT+TGG 0.516294 1.1:-8399015 None:intergenic
TGTGATGTTGAAGGAGTAGG+AGG 0.527946 1.1:-8398749 None:intergenic
TTAGGCTTAGGAGATGGAGT+TGG 0.532997 1.1:-8398659 None:intergenic
TTTGGCTTAGGCTTAGGAGA+TGG 0.535668 1.1:-8398665 None:intergenic
GCACAACACTTGCTAGAAGC+AGG 0.549118 1.1:-8398848 None:intergenic
GTGAAAATGTTGCATAAGCA+AGG 0.554272 1.1:-8398616 None:intergenic
CCTACTGACACATTAAAGCT+AGG 0.564359 1.1:+8398779 MS.gene60296:CDS
CATGGTGTTGGTGAAGCTGG+TGG 0.586112 1.1:-8398704 None:intergenic
GGAGTAGGAGGTGTTGCTGA+AGG 0.590328 1.1:-8398737 None:intergenic
GGTGTTGGAGGACATGGTGT+TGG 0.594079 1.1:-8398716 None:intergenic
GGTGTTGGTGAAGCTGGTGG+AGG 0.615450 1.1:-8398701 None:intergenic
CCTAGCTTTAATGTGTCAGT+AGG 0.615781 1.1:-8398779 None:intergenic
GGTGTTGCTGAAGGTGTTGG+AGG 0.621506 1.1:-8398728 None:intergenic
TAGCCTTAATAGCAGTACAA+AGG 0.655083 1.1:-8398910 None:intergenic
TATGAACATTGGAAACCCGA+AGG 0.685560 1.1:-8399004 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GAGAGAAAATAATATAATTA+GGG - chr1.1:8398597-8398616 None:intergenic 15.0%
!! GGAGAGAAAATAATATAATT+AGG - chr1.1:8398598-8398617 None:intergenic 20.0%
!!! GGACTTGTTAATATTATTGT+TGG + chr1.1:8398821-8398840 MS.gene60296:CDS 25.0%
TTGTGAAAAATCTGTTCCTT+CGG + chr1.1:8398988-8399007 MS.gene60296:CDS 30.0%
! ATTTTTCACAAGCACTAAGT+AGG - chr1.1:8398979-8398998 None:intergenic 30.0%
GCTATTAAGGCTAATGTTCT+TGG + chr1.1:8398920-8398939 MS.gene60296:CDS 35.0%
GTGAAAATGTTGCATAAGCA+AGG - chr1.1:8398619-8398638 None:intergenic 35.0%
TGTGAAAAATCTGTTCCTTC+GGG + chr1.1:8398989-8399008 MS.gene60296:CDS 35.0%
TTGCCTTTGTACTGCTATTA+AGG + chr1.1:8398907-8398926 MS.gene60296:CDS 35.0%
! GGACATTTTTGTGATGTTGA+AGG - chr1.1:8398761-8398780 None:intergenic 35.0%
! TAGCCTTAATAGCAGTACAA+AGG - chr1.1:8398913-8398932 None:intergenic 35.0%
! TTTTGTGATGTTGAAGGAGT+AGG - chr1.1:8398755-8398774 None:intergenic 35.0%
CCTACTGACACATTAAAGCT+AGG + chr1.1:8398779-8398798 MS.gene60296:CDS 40.0%
TATGAACATTGGAAACCCGA+AGG - chr1.1:8399007-8399026 None:intergenic 40.0%
TTGTGCATTGCTTCAAGGTT+TGG + chr1.1:8398865-8398884 MS.gene60296:CDS 40.0%
! AAGTGTTGTGCATTGCTTCA+AGG + chr1.1:8398860-8398879 MS.gene60296:CDS 40.0%
! AATTAGGGCACTAAGCTTGT+TGG - chr1.1:8398582-8398601 None:intergenic 40.0%
! GCAACATTTTCACATGCTTG+TGG + chr1.1:8398626-8398645 MS.gene60296:CDS 40.0%
! GCTTCAAGGTTTGGTTGATT+TGG + chr1.1:8398874-8398893 MS.gene60296:CDS 40.0%
!! CCTAGCTTTAATGTGTCAGT+AGG - chr1.1:8398782-8398801 None:intergenic 40.0%
AGTAGGAGACTGAGTGTGAT+AGG - chr1.1:8398962-8398981 None:intergenic 45.0%
TGTGATGTTGAAGGAGTAGG+AGG - chr1.1:8398752-8398771 None:intergenic 45.0%
TTAGGCTTAGGAGATGGAGT+TGG - chr1.1:8398662-8398681 None:intergenic 45.0%
! TTTGGCTTAGGCTTAGGAGA+TGG - chr1.1:8398668-8398687 None:intergenic 45.0%
!! TTTGGCTTTGGCTTAGGCTT+AGG - chr1.1:8398674-8398693 None:intergenic 45.0%
! GCACAACACTTGCTAGAAGC+AGG - chr1.1:8398851-8398870 None:intergenic 50.0%
! GGTGTGTGTGCTGATGTTCT+AGG + chr1.1:8398800-8398819 MS.gene60296:CDS 50.0%
!! CTAGGCTTTGGCTTTGGCTT+AGG - chr1.1:8398680-8398699 None:intergenic 50.0%
! GGAGGACTAGGCTTTGGCTT+TGG - chr1.1:8398686-8398705 None:intergenic 55.0%
! GGTGTTGGAGGACATGGTGT+TGG - chr1.1:8398719-8398738 None:intergenic 55.0%
!! CATGGTGTTGGTGAAGCTGG+TGG - chr1.1:8398707-8398726 None:intergenic 55.0%
!! GCTGAAGGTGTTGGAGGACA+TGG - chr1.1:8398725-8398744 None:intergenic 55.0%
!! GGACATGGTGTTGGTGAAGC+TGG - chr1.1:8398710-8398729 None:intergenic 55.0%
!! GGAGGTGTTGCTGAAGGTGT+TGG - chr1.1:8398734-8398753 None:intergenic 55.0%
!! GGAGTAGGAGGTGTTGCTGA+AGG - chr1.1:8398740-8398759 None:intergenic 55.0%
!! GGTGTTGCTGAAGGTGTTGG+AGG - chr1.1:8398731-8398750 None:intergenic 55.0%
GCTGGTGGAGGACTAGGCTT+TGG - chr1.1:8398692-8398711 None:intergenic 60.0%
GGTGAAGCTGGTGGAGGACT+AGG - chr1.1:8398698-8398717 None:intergenic 60.0%
!! GGTGTTGGTGAAGCTGGTGG+AGG - chr1.1:8398704-8398723 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr1.1 gene 8398572 8399027 8398572 ID=MS.gene60296
chr1.1 mRNA 8398572 8399027 8398572 ID=MS.gene60296.t1;Parent=MS.gene60296
chr1.1 exon 8398572 8399027 8398572 ID=MS.gene60296.t1.exon1;Parent=MS.gene60296.t1
chr1.1 CDS 8398572 8399027 8398572 ID=cds.MS.gene60296.t1;Parent=MS.gene60296.t1
Gene Sequence

>MS.gene60296

ATGGCTTCCAACAAGCTTAGTGCCCTAATTATATTATTTTCTCTCCTTGCTTATGCAACATTTTCACATGCTTGTGGTTCATGCAAACCAACTCCATCTCCTAAGCCTAAGCCAAAGCCAAAGCCTAGTCCTCCACCAGCTTCACCAACACCATGTCCTCCAACACCTTCAGCAACACCTCCTACTCCTTCAACATCACAAAAATGTCCTACTGACACATTAAAGCTAGGTGTGTGTGCTGATGTTCTAGGACTTGTTAATATTATTGTTGGAAGTCCTGCTTCTAGCAAGTGTTGTGCATTGCTTCAAGGTTTGGTTGATTTGGATGCAGCAATTTGCCTTTGTACTGCTATTAAGGCTAATGTTCTTGGTATCAACTTGAATGTTCCTATCACACTCAGTCTCCTACTTAGTGCTTGTGAAAAATCTGTTCCTTCGGGTTTCCAATGTTCATAG

Protein sequence

>MS.gene60296.t1

MASNKLSALIILFSLLAYATFSHACGSCKPTPSPKPKPKPKPSPPPASPTPCPPTPSATPPTPSTSQKCPTDTLKLGVCADVLGLVNIIVGSPASSKCCALLQGLVDLDAAICLCTAIKANVLGINLNVPITLSLLLSACEKSVPSGFQCS