AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene072048


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene072048.t1 MTR_2g006290 91.503 153 10 1 1 153 1 150 8.66e-101 286
MS.gene072048.t1 MTR_8g088720 61.290 155 46 3 3 153 4 148 4.01e-62 188
MS.gene072048.t1 MTR_1g112030 55.333 150 58 3 3 151 14 155 6.82e-51 160
MS.gene072048.t1 MTR_1g112030 55.333 150 58 3 3 151 18 159 7.08e-51 160
MS.gene072048.t1 MTR_4g053120 58.519 135 42 3 28 151 23 154 1.23e-50 159
MS.gene072048.t1 MTR_1g112050 55.705 149 53 4 6 151 15 153 1.04e-49 157
MS.gene072048.t1 MTR_2g086510 61.157 121 43 2 32 151 37 154 5.71e-48 152
MS.gene072048.t1 MTR_5g006320 38.947 95 57 1 57 151 50 143 2.06e-17 74.3
MS.gene072048.t1 MTR_4g098490 39.024 82 50 0 70 151 77 158 1.30e-16 73.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene072048.t1 AT1G60950 64.626 147 47 3 6 151 5 147 3.88e-61 186
MS.gene072048.t1 AT1G10960 63.699 146 50 2 6 151 5 147 7.43e-55 169
MS.gene072048.t1 AT2G27510 50.318 157 69 4 1 151 1 154 2.17e-45 146
MS.gene072048.t1 AT5G10000 43.662 142 75 3 14 151 7 147 1.09e-34 118
MS.gene072048.t1 AT4G14890 34.043 141 82 3 21 151 5 144 1.25e-18 77.8
MS.gene072048.t1 AT1G32550 37.037 108 68 0 44 151 46 153 5.63e-18 76.6
MS.gene072048.t1 AT1G32550 34.677 124 62 3 44 151 46 166 2.71e-12 62.0

Find 34 sgRNAs with CRISPR-Local

Find 39 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCAAATTCTTGAGTTCCTTC+TGG 0.323416 2.4:-76079069 None:intergenic
GAACCAGCTCTGCATGAGTA+TGG 0.373256 2.4:-76079147 None:intergenic
ATGATCAGATTGAAGAAGGT+TGG 0.396228 2.4:+76079238 MS.gene072048:CDS
TTCTTCCTTGTGAGTCTCAA+TGG 0.400867 2.4:-76079298 None:intergenic
TCAAGAATGTAAACATCATC+TGG 0.411090 2.4:-76079096 None:intergenic
GGTTCTTGTTCTTCATGTGC+TGG 0.425033 2.4:+76079165 MS.gene072048:CDS
ACCGCTGGGAAAAGCCTTTG+TGG 0.440903 2.4:-76078968 None:intergenic
GCAACCGCACCTGCTTTGTA+TGG 0.461704 2.4:+76078877 MS.gene072048:CDS
GTGCTGACTGACACTGGCAT+TGG 0.464692 2.4:-76078934 None:intergenic
TGATCAGATTGAAGAAGGTT+GGG 0.468092 2.4:+76079239 MS.gene072048:CDS
CTTCCATACTCATGCAGAGC+TGG 0.477015 2.4:+76079144 MS.gene072048:CDS
CTTGACCATGCTGAGGAAGT+TGG 0.477077 2.4:+76079114 MS.gene072048:CDS
CATTGGCTGCCTCCTGAGGA+AGG 0.479539 2.4:-76078917 None:intergenic
AGTTGCAATGGCTAGTTACA+AGG 0.482157 2.4:+76079032 MS.gene072048:CDS
CAGTGAGCACCTCCTTCCTC+AGG 0.501999 2.4:+76078905 MS.gene072048:CDS
AGCCTTTGTGGTTGTTGTGG+TGG 0.506920 2.4:-76078956 None:intergenic
CTTCAATCTGATCATCATCA+AGG 0.519281 2.4:-76079230 None:intergenic
TGTGCTGGCAAAGTTGCTGC+TGG 0.520711 2.4:+76079180 MS.gene072048:CDS
AAAAGCCTTTGTGGTTGTTG+TGG 0.532495 2.4:-76078959 None:intergenic
GATGATGATCAGATTGAAGA+AGG 0.539674 2.4:+76079234 MS.gene072048:CDS
GTGGTGGTGCTGACTGACAC+TGG 0.553388 2.4:-76078940 None:intergenic
CTGGCATTGGCTGCCTCCTG+AGG 0.569091 2.4:-76078921 None:intergenic
TGGCTGCCTCCTGAGGAAGG+AGG 0.573079 2.4:-76078914 None:intergenic
ACTGCAGTTCCATACAAAGC+AGG 0.605761 2.4:-76078886 None:intergenic
GTGAAGCTTGTTACACCAGA+AGG 0.613290 2.4:+76079054 MS.gene072048:CDS
TGAGCACCTCCTTCCTCAGG+AGG 0.621811 2.4:+76078908 MS.gene072048:CDS
TGGTGATCTTGCAGTTGCAA+TGG 0.626955 2.4:+76079020 MS.gene072048:CDS
AAAAGTAACACAGGAAAGCG+TGG 0.634896 2.4:+76079000 MS.gene072048:CDS
TTACATTCTTGACCATGCTG+AGG 0.655914 2.4:+76079107 MS.gene072048:CDS
CAATACCAACTTCCTCAGCA+TGG 0.657416 2.4:-76079119 None:intergenic
GGTGAAGTTGATCAGTCAGA+TGG 0.657495 2.4:+76079201 MS.gene072048:CDS
CACCACCACAACAACCACAA+AGG 0.670170 2.4:+76078954 MS.gene072048:CDS
AGTTCCATACAAAGCAGGTG+CGG 0.688139 2.4:-76078881 None:intergenic
TGTTACCATTGAGACTCACA+AGG 0.695016 2.4:+76079293 MS.gene072048:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTTGGTTTAAAAAGTAACAC+AGG + chr2.4:76078991-76079010 MS.gene072048:CDS 25.0%
TCAAGAATGTAAACATCATC+TGG - chr2.4:76079099-76079118 None:intergenic 30.0%
ATGATCAGATTGAAGAAGGT+TGG + chr2.4:76079238-76079257 MS.gene072048:CDS 35.0%
CTTCAATCTGATCATCATCA+AGG - chr2.4:76079233-76079252 None:intergenic 35.0%
GATGATGATCAGATTGAAGA+AGG + chr2.4:76079234-76079253 MS.gene072048:CDS 35.0%
TCAAATTCTTGAGTTCCTTC+TGG - chr2.4:76079072-76079091 None:intergenic 35.0%
TGATCAGATTGAAGAAGGTT+GGG + chr2.4:76079239-76079258 MS.gene072048:CDS 35.0%
! ACTTTTTAAACCAAAACCGC+TGG - chr2.4:76078986-76079005 None:intergenic 35.0%
! CTTTTTAAACCAAAACCGCT+GGG - chr2.4:76078985-76079004 None:intergenic 35.0%
AAAAGCCTTTGTGGTTGTTG+TGG - chr2.4:76078962-76078981 None:intergenic 40.0%
AAAAGTAACACAGGAAAGCG+TGG + chr2.4:76079000-76079019 MS.gene072048:CDS 40.0%
TGTTACCATTGAGACTCACA+AGG + chr2.4:76079293-76079312 MS.gene072048:CDS 40.0%
TTACATTCTTGACCATGCTG+AGG + chr2.4:76079107-76079126 MS.gene072048:CDS 40.0%
TTCTTCCTTGTGAGTCTCAA+TGG - chr2.4:76079301-76079320 None:intergenic 40.0%
! AGTTGCAATGGCTAGTTACA+AGG + chr2.4:76079032-76079051 MS.gene072048:CDS 40.0%
ACTGCAGTTCCATACAAAGC+AGG - chr2.4:76078889-76078908 None:intergenic 45.0%
AGTTCCATACAAAGCAGGTG+CGG - chr2.4:76078884-76078903 None:intergenic 45.0%
CAATACCAACTTCCTCAGCA+TGG - chr2.4:76079122-76079141 None:intergenic 45.0%
GGTTCTTGTTCTTCATGTGC+TGG + chr2.4:76079165-76079184 MS.gene072048:CDS 45.0%
TGGTGATCTTGCAGTTGCAA+TGG + chr2.4:76079020-76079039 MS.gene072048:CDS 45.0%
! GGTGAAGTTGATCAGTCAGA+TGG + chr2.4:76079201-76079220 MS.gene072048:CDS 45.0%
!! GTGAAGCTTGTTACACCAGA+AGG + chr2.4:76079054-76079073 MS.gene072048:CDS 45.0%
AGCCTTTGTGGTTGTTGTGG+TGG - chr2.4:76078959-76078978 None:intergenic 50.0%
CACCACCACAACAACCACAA+AGG + chr2.4:76078954-76078973 MS.gene072048:CDS 50.0%
CTTCCATACTCATGCAGAGC+TGG + chr2.4:76079144-76079163 MS.gene072048:CDS 50.0%
CTTGACCATGCTGAGGAAGT+TGG + chr2.4:76079114-76079133 MS.gene072048:CDS 50.0%
GAACCAGCTCTGCATGAGTA+TGG - chr2.4:76079150-76079169 None:intergenic 50.0%
! AAGGCTTTTCCCAGCGGTTT+TGG + chr2.4:76078973-76078992 MS.gene072048:CDS 50.0%
! ACCACAAAGGCTTTTCCCAG+CGG + chr2.4:76078967-76078986 MS.gene072048:CDS 50.0%
ACCGCTGGGAAAAGCCTTTG+TGG - chr2.4:76078971-76078990 None:intergenic 55.0%
GCAACCGCACCTGCTTTGTA+TGG + chr2.4:76078877-76078896 MS.gene072048:CDS 55.0%
! TGTGCTGGCAAAGTTGCTGC+TGG + chr2.4:76079180-76079199 MS.gene072048:CDS 55.0%
!! GTGCTGACTGACACTGGCAT+TGG - chr2.4:76078937-76078956 None:intergenic 55.0%
CAGTGAGCACCTCCTTCCTC+AGG + chr2.4:76078905-76078924 MS.gene072048:CDS 60.0%
TGAGCACCTCCTTCCTCAGG+AGG + chr2.4:76078908-76078927 MS.gene072048:CDS 60.0%
! CATTGGCTGCCTCCTGAGGA+AGG - chr2.4:76078920-76078939 None:intergenic 60.0%
!! GTGGTGGTGCTGACTGACAC+TGG - chr2.4:76078943-76078962 None:intergenic 60.0%
TGGCTGCCTCCTGAGGAAGG+AGG - chr2.4:76078917-76078936 None:intergenic 65.0%
!! CTGGCATTGGCTGCCTCCTG+AGG - chr2.4:76078924-76078943 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 76078874 76079335 76078874 ID=MS.gene072048
chr2.4 mRNA 76078874 76079335 76078874 ID=MS.gene072048.t1;Parent=MS.gene072048
chr2.4 exon 76078874 76079335 76078874 ID=MS.gene072048.t1.exon1;Parent=MS.gene072048.t1
chr2.4 CDS 76078874 76079335 76078874 ID=cds.MS.gene072048.t1;Parent=MS.gene072048.t1
Gene Sequence

>MS.gene072048

ATGGCAACCGCACCTGCTTTGTATGGAACTGCAGTGAGCACCTCCTTCCTCAGGAGGCAGCCAATGCCAGTGTCAGTCAGCACCACCACAACAACCACAAAGGCTTTTCCCAGCGGTTTTGGTTTAAAAAGTAACACAGGAAAGCGTGGTGATCTTGCAGTTGCAATGGCTAGTTACAAGGTGAAGCTTGTTACACCAGAAGGAACTCAAGAATTTGAATGTCCAGATGATGTTTACATTCTTGACCATGCTGAGGAAGTTGGTATTGAACTTCCATACTCATGCAGAGCTGGTTCTTGTTCTTCATGTGCTGGCAAAGTTGCTGCTGGTGAAGTTGATCAGTCAGATGGTTCTTTCCTTGATGATGATCAGATTGAAGAAGGTTGGGTTCTTACATGTGTTGCTTATGCTAAGTCTGATGTTACCATTGAGACTCACAAGGAAGAAGAACTCACTGCTTAA

Protein sequence

>MS.gene072048.t1

MATAPALYGTAVSTSFLRRQPMPVSVSTTTTTTKAFPSGFGLKSNTGKRGDLAVAMASYKVKLVTPEGTQEFECPDDVYILDHAEEVGIELPYSCRAGSCSSCAGKVAAGEVDQSDGSFLDDDQIEEGWVLTCVAYAKSDVTIETHKEEELTA