AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene68130


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene68130.t1 MTR_3g118210 99.558 226 1 0 1 226 47 272 9.90e-165 455
MS.gene68130.t1 MTR_6g006970 42.718 206 114 4 12 216 49 251 9.85e-47 156
MS.gene68130.t1 MTR_1g093060 40.777 206 118 4 12 216 102 304 1.06e-42 147
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene68130.t1 AT2G43030 82.743 226 39 0 1 226 46 271 4.47e-130 368
MS.gene68130.t1 AT3G17465 37.037 216 133 3 11 225 77 290 2.47e-38 135

Find 78 sgRNAs with CRISPR-Local

Find 89 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTAACAATCTTAAGCTTTC+TGG 0.097361 3.1:-92902277 None:intergenic
AAAGAGACTAATTAATTCTT+TGG 0.209215 3.1:-92902402 None:intergenic
AAAGCTCATCATTCCCAATT+TGG 0.228676 3.1:-92901687 None:intergenic
CAAATTGACCAAACCTGAAA+TGG 0.282604 3.1:+92901844 MS.gene68130:CDS
CATTCCTATGCGACATCTTC+AGG 0.315485 3.1:+92901889 MS.gene68130:CDS
ATGATCAAAGGTGCTCTTCC+TGG 0.365904 3.1:+92902324 MS.gene68130:CDS
TTTCGTCTTCAATCCGTTGA+TGG 0.384156 3.1:+92901914 MS.gene68130:CDS
TCAAATACAAGGCGCTGATT+AGG 0.398930 3.1:-92901944 None:intergenic
AAGATGACCCATTTCAGGTT+TGG 0.399688 3.1:-92901852 None:intergenic
CTCTTGGAACCATGGCTCAT+TGG 0.403785 3.1:-92902156 None:intergenic
TGGTGTGATGGGTACCAAAT+TGG 0.414953 3.1:+92901673 MS.gene68130:CDS
GTTGTTCCCGTCACTGTTGT+CGG 0.415769 3.1:+92901728 MS.gene68130:CDS
GGTGATCTTGTTGATGTCTC+TGG 0.428399 3.1:+92901983 MS.gene68130:CDS
AAGCTTTCTGGTCTTCCTGT+TGG 0.434373 3.1:-92902265 None:intergenic
ACGCCGTCCAAGTTGGTTAC+CGG 0.438083 3.1:+92901807 MS.gene68130:CDS
TCCAAGTTGTCTGTGACTCT+TGG 0.442433 3.1:-92902172 None:intergenic
TCCAAGAGTCACAGACAACT+TGG 0.460086 3.1:+92902171 MS.gene68130:CDS
TTCTTGCCAACAATCTTAGC+GGG 0.460342 3.1:-92902375 None:intergenic
ACTGTTGTCGGTTTCAAAGA+AGG 0.461503 3.1:+92901740 MS.gene68130:CDS
AGAGGCTGGTGTTGGTGTGA+TGG 0.463975 3.1:+92901661 MS.gene68130:CDS
TACACCTGGTCGCGTATACA+AGG 0.469173 3.1:+92902212 MS.gene68130:CDS
TTTCAGGTTTGGTCAATTTG+CGG 0.469193 3.1:-92901841 None:intergenic
GTTGTAACCGTCGGTGGCGT+CGG 0.473671 3.1:-92901786 None:intergenic
AAAGAAGATGCCTGGTAGGA+TGG 0.474224 3.1:+92902236 MS.gene68130:CDS
GAGGCTGGTGTTGGTGTGAT+GGG 0.474712 3.1:+92901662 MS.gene68130:CDS
TAACCCTCCGGTAACCAACT+TGG 0.478957 3.1:-92901814 None:intergenic
CACAGACAACTTGGATCCAT+TGG 0.481197 3.1:+92902180 MS.gene68130:CDS
GTAGGATGGGTGGAACCAAC+AGG 0.493432 3.1:+92902250 MS.gene68130:CDS
TACGCGACCAGGTGTAGTTC+CGG 0.494636 3.1:-92902205 None:intergenic
TTTCCCTTGTATACGCGACC+AGG 0.499382 3.1:-92902216 None:intergenic
AGAAGATTACCAGGCTTACC+AGG 0.500896 3.1:-92902342 None:intergenic
TGAAATGGGTCATCTTGAAA+AGG 0.503372 3.1:+92901859 MS.gene68130:CDS
ACGGGAACAACTTCACCGTT+AGG 0.508452 3.1:-92901716 None:intergenic
AAGAAGATGCCTGGTAGGAT+GGG 0.509948 3.1:+92902237 MS.gene68130:CDS
GGTGTGATGGGTACCAAATT+GGG 0.512034 3.1:+92901674 MS.gene68130:CDS
AAATTGACCAAACCTGAAAT+GGG 0.513032 3.1:+92901845 MS.gene68130:CDS
TTGAAACCGACAACAGTGAC+GGG 0.513527 3.1:-92901734 None:intergenic
GGTGCTCTTCCTGGTAAGCC+TGG 0.514459 3.1:+92902333 MS.gene68130:CDS
AAGCGTCATAACTTCAAAAG+AGG 0.517261 3.1:+92902132 MS.gene68130:CDS
AGGTGTAGTTCCGGCACCAA+TGG 0.518535 3.1:-92902196 None:intergenic
TTGGTTCCACCCATCCTACC+AGG 0.519178 3.1:-92902246 None:intergenic
ATGGGTCATCTTGAAAAGGT+TGG 0.519826 3.1:+92901863 MS.gene68130:CDS
GATGTCTCTGGCACCACCAT+CGG 0.522268 3.1:+92901995 MS.gene68130:CDS
GGTGGTGAAAATGAGCTTAG+AGG 0.522460 3.1:+92901643 MS.gene68130:CDS
GTAAGCCTGGTAATCTTCTA+CGG 0.530779 3.1:+92902346 MS.gene68130:CDS
GAACAACTTCACCGTTAGGT+TGG 0.533148 3.1:-92901712 None:intergenic
CTCTGGCACCACCATCGGCA+AGG 0.534330 3.1:+92902000 MS.gene68130:CDS
TACAAGGGAAAGAAGATGCC+TGG 0.536322 3.1:+92902228 MS.gene68130:CDS
GTCTGTGACTCTTGGAACCA+TGG 0.543733 3.1:-92902164 None:intergenic
CTGAAGATGTCGCATAGGAA+TGG 0.560330 3.1:-92901888 None:intergenic
CAACTTGGATCCATTGGTGC+CGG 0.569215 3.1:+92902186 MS.gene68130:CDS
ATTTGCGGTCTCTAACCCTC+CGG 0.569415 3.1:-92901826 None:intergenic
ATTAGGTTCAAAACCATCAA+CGG 0.570807 3.1:-92901927 None:intergenic
TCTGGCACCACCATCGGCAA+GGG 0.572275 3.1:+92902001 MS.gene68130:CDS
CTTAATGTTATCATGATCAA+AGG 0.572918 3.1:+92902312 MS.gene68130:CDS
ATTGGTGCCGGAACTACACC+TGG 0.580799 3.1:+92902198 MS.gene68130:CDS
TTTGAAACCGACAACAGTGA+CGG 0.586413 3.1:-92901735 None:intergenic
CCGTCCAAGTTGGTTACCGG+AGG 0.591201 3.1:+92901810 MS.gene68130:CDS
GTTCTTGCCAACAATCTTAG+CGG 0.594511 3.1:-92902376 None:intergenic
AAGATGCCTGGTAGGATGGG+TGG 0.594546 3.1:+92902240 MS.gene68130:CDS
ACCTTGAAAACCCTTGCCGA+TGG 0.600155 3.1:-92902011 None:intergenic
TGAAAAGCTCATCAAATACA+AGG 0.600821 3.1:-92901955 None:intergenic
GCTATCCGTAGAAGATTACC+AGG 0.602366 3.1:-92902351 None:intergenic
AGGGAAAGAAGATGCCTGGT+AGG 0.605040 3.1:+92902232 MS.gene68130:CDS
TTGAAAACCCTTGCCGATGG+TGG 0.607518 3.1:-92902008 None:intergenic
ATCAAAACCGACGCCACCGA+CGG 0.609949 3.1:+92901779 MS.gene68130:CDS
GGTTACAACGCCGTCCAAGT+TGG 0.618166 3.1:+92901800 MS.gene68130:CDS
GTGAAAATGAGCTTAGAGGC+TGG 0.634928 3.1:+92901647 MS.gene68130:CDS
TCTTGCCAACAATCTTAGCG+GGG 0.638407 3.1:-92902374 None:intergenic
CCTCCGGTAACCAACTTGGA+CGG 0.639997 3.1:-92901810 None:intergenic
ATGAGCTTAGAGGCTGGTGT+TGG 0.640133 3.1:+92901653 MS.gene68130:CDS
AAAAGAGGACCAATGAGCCA+TGG 0.640937 3.1:+92902147 MS.gene68130:CDS
CGTCCAAGTTGGTTACCGGA+GGG 0.643249 3.1:+92901811 MS.gene68130:CDS
CTTGCCAACAATCTTAGCGG+GGG 0.643846 3.1:-92902373 None:intergenic
GACGGCGTTGTAACCGTCGG+TGG 0.648118 3.1:-92901792 None:intergenic
AACTCCTGAAGATGTCGCAT+AGG 0.655881 3.1:-92901893 None:intergenic
TTGGACGGCGTTGTAACCGT+CGG 0.663802 3.1:-92901795 None:intergenic
ACACCTGGTCGCGTATACAA+GGG 0.687415 3.1:+92902213 MS.gene68130:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAATAATATCTTTCACTAA+TGG + chr3.1:92902079-92902098 MS.gene68130:intron 15.0%
! CTTAATGTTATCATGATCAA+AGG + chr3.1:92902312-92902331 MS.gene68130:CDS 25.0%
! TTTAACAATCTTAAGCTTTC+TGG - chr3.1:92902280-92902299 None:intergenic 25.0%
AAATTGACCAAACCTGAAAT+GGG + chr3.1:92901845-92901864 MS.gene68130:CDS 30.0%
ATGAACTTACTTACTTTGAC+AGG + chr3.1:92902102-92902121 MS.gene68130:intron 30.0%
ATTAGGTTCAAAACCATCAA+CGG - chr3.1:92901930-92901949 None:intergenic 30.0%
TATCAACTCAACAACTAGAA+GGG - chr3.1:92902062-92902081 None:intergenic 30.0%
TGAAAAGCTCATCAAATACA+AGG - chr3.1:92901958-92901977 None:intergenic 30.0%
TTATCAACTCAACAACTAGA+AGG - chr3.1:92902063-92902082 None:intergenic 30.0%
! TTTGATGAGCTTTTCAATGA+AGG + chr3.1:92901962-92901981 MS.gene68130:CDS 30.0%
AAAGCTCATCATTCCCAATT+TGG - chr3.1:92901690-92901709 None:intergenic 35.0%
AACTTACTTACTTTGACAGG+TGG + chr3.1:92902105-92902124 MS.gene68130:intron 35.0%
AAGCGTCATAACTTCAAAAG+AGG + chr3.1:92902132-92902151 MS.gene68130:CDS 35.0%
CAAATTGACCAAACCTGAAA+TGG + chr3.1:92901844-92901863 MS.gene68130:CDS 35.0%
TGAAATGGGTCATCTTGAAA+AGG + chr3.1:92901859-92901878 MS.gene68130:CDS 35.0%
!! ATGAGCTTTTTCCAACCTAA+CGG + chr3.1:92901701-92901720 MS.gene68130:CDS 35.0%
!! TTTCAGGTTTGGTCAATTTG+CGG - chr3.1:92901844-92901863 None:intergenic 35.0%
!! TTTTCAAGATGACCCATTTC+AGG - chr3.1:92901860-92901879 None:intergenic 35.0%
AACTAGAAGGGAATGCAAAG+AGG - chr3.1:92902050-92902069 None:intergenic 40.0%
ACTAGAAGGGAATGCAAAGA+GGG - chr3.1:92902049-92902068 None:intergenic 40.0%
ATGGGTCATCTTGAAAAGGT+TGG + chr3.1:92901863-92901882 MS.gene68130:CDS 40.0%
GTAAGCCTGGTAATCTTCTA+CGG + chr3.1:92902346-92902365 MS.gene68130:CDS 40.0%
GTTCTTGCCAACAATCTTAG+CGG - chr3.1:92902379-92902398 None:intergenic 40.0%
TTCTTGCCAACAATCTTAGC+GGG - chr3.1:92902378-92902397 None:intergenic 40.0%
TTTCGTCTTCAATCCGTTGA+TGG + chr3.1:92901914-92901933 MS.gene68130:CDS 40.0%
! TTTGAAACCGACAACAGTGA+CGG - chr3.1:92901738-92901757 None:intergenic 40.0%
!! AAGATGACCCATTTCAGGTT+TGG - chr3.1:92901855-92901874 None:intergenic 40.0%
!! ACTGTTGTCGGTTTCAAAGA+AGG + chr3.1:92901740-92901759 MS.gene68130:CDS 40.0%
!! TCAAATACAAGGCGCTGATT+AGG - chr3.1:92901947-92901966 None:intergenic 40.0%
AAAAGAGGACCAATGAGCCA+TGG + chr3.1:92902147-92902166 MS.gene68130:CDS 45.0%
AAAGAAGATGCCTGGTAGGA+TGG + chr3.1:92902236-92902255 MS.gene68130:CDS 45.0%
AACTCCTGAAGATGTCGCAT+AGG - chr3.1:92901896-92901915 None:intergenic 45.0%
AAGAAGATGCCTGGTAGGAT+GGG + chr3.1:92902237-92902256 MS.gene68130:CDS 45.0%
AGAAGATTACCAGGCTTACC+AGG - chr3.1:92902345-92902364 None:intergenic 45.0%
CACAGACAACTTGGATCCAT+TGG + chr3.1:92902180-92902199 MS.gene68130:CDS 45.0%
CATTCCTATGCGACATCTTC+AGG + chr3.1:92901889-92901908 MS.gene68130:CDS 45.0%
CTGAAGATGTCGCATAGGAA+TGG - chr3.1:92901891-92901910 None:intergenic 45.0%
GAACAACTTCACCGTTAGGT+TGG - chr3.1:92901715-92901734 None:intergenic 45.0%
GCTATCCGTAGAAGATTACC+AGG - chr3.1:92902354-92902373 None:intergenic 45.0%
GGTGATCTTGTTGATGTCTC+TGG + chr3.1:92901983-92902002 MS.gene68130:CDS 45.0%
GGTGGTGAAAATGAGCTTAG+AGG + chr3.1:92901643-92901662 MS.gene68130:CDS 45.0%
GTGAAAATGAGCTTAGAGGC+TGG + chr3.1:92901647-92901666 MS.gene68130:CDS 45.0%
TACAAGGGAAAGAAGATGCC+TGG + chr3.1:92902228-92902247 MS.gene68130:CDS 45.0%
TCCAAGAGTCACAGACAACT+TGG + chr3.1:92902171-92902190 MS.gene68130:CDS 45.0%
TCCAAGTTGTCTGTGACTCT+TGG - chr3.1:92902175-92902194 None:intergenic 45.0%
TCTTGCCAACAATCTTAGCG+GGG - chr3.1:92902377-92902396 None:intergenic 45.0%
TTGAAACCGACAACAGTGAC+GGG - chr3.1:92901737-92901756 None:intergenic 45.0%
! AAGCTTTCTGGTCTTCCTGT+TGG - chr3.1:92902268-92902287 None:intergenic 45.0%
!! ATGATCAAAGGTGCTCTTCC+TGG + chr3.1:92902324-92902343 MS.gene68130:CDS 45.0%
!! GGTGTGATGGGTACCAAATT+GGG + chr3.1:92901674-92901693 MS.gene68130:CDS 45.0%
!! TGGTGTGATGGGTACCAAAT+TGG + chr3.1:92901673-92901692 MS.gene68130:CDS 45.0%
ACACCTGGTCGCGTATACAA+GGG + chr3.1:92902213-92902232 MS.gene68130:CDS 50.0%
ACCTTGAAAACCCTTGCCGA+TGG - chr3.1:92902014-92902033 None:intergenic 50.0%
ACGGGAACAACTTCACCGTT+AGG - chr3.1:92901719-92901738 None:intergenic 50.0%
AGGGAAAGAAGATGCCTGGT+AGG + chr3.1:92902232-92902251 MS.gene68130:CDS 50.0%
ATAGCCCCCGCTAAGATTGT+TGG + chr3.1:92902369-92902388 MS.gene68130:CDS 50.0%
ATGAGCTTAGAGGCTGGTGT+TGG + chr3.1:92901653-92901672 MS.gene68130:CDS 50.0%
ATTTGCGGTCTCTAACCCTC+CGG - chr3.1:92901829-92901848 None:intergenic 50.0%
CAACTTGGATCCATTGGTGC+CGG + chr3.1:92902186-92902205 MS.gene68130:CDS 50.0%
CTCTTGGAACCATGGCTCAT+TGG - chr3.1:92902159-92902178 None:intergenic 50.0%
CTTGCCAACAATCTTAGCGG+GGG - chr3.1:92902376-92902395 None:intergenic 50.0%
GTCTGTGACTCTTGGAACCA+TGG - chr3.1:92902167-92902186 None:intergenic 50.0%
GTTGTTCCCGTCACTGTTGT+CGG + chr3.1:92901728-92901747 MS.gene68130:CDS 50.0%
TAACCCTCCGGTAACCAACT+TGG - chr3.1:92901817-92901836 None:intergenic 50.0%
TACACCTGGTCGCGTATACA+AGG + chr3.1:92902212-92902231 MS.gene68130:CDS 50.0%
TTGAAAACCCTTGCCGATGG+TGG - chr3.1:92902011-92902030 None:intergenic 50.0%
TTTCCCTTGTATACGCGACC+AGG - chr3.1:92902219-92902238 None:intergenic 50.0%
! ACCATCGGCAAGGGTTTTCA+AGG + chr3.1:92902010-92902029 MS.gene68130:CDS 50.0%
AAGATGCCTGGTAGGATGGG+TGG + chr3.1:92902240-92902259 MS.gene68130:CDS 55.0%
AGGTGTAGTTCCGGCACCAA+TGG - chr3.1:92902199-92902218 None:intergenic 55.0%
ATCAAAACCGACGCCACCGA+CGG + chr3.1:92901779-92901798 MS.gene68130:CDS 55.0%
CCTCCGGTAACCAACTTGGA+CGG - chr3.1:92901813-92901832 None:intergenic 55.0%
GATGTCTCTGGCACCACCAT+CGG + chr3.1:92901995-92902014 MS.gene68130:CDS 55.0%
GGTTACAACGCCGTCCAAGT+TGG + chr3.1:92901800-92901819 MS.gene68130:CDS 55.0%
GTAGGATGGGTGGAACCAAC+AGG + chr3.1:92902250-92902269 MS.gene68130:CDS 55.0%
TACGCGACCAGGTGTAGTTC+CGG - chr3.1:92902208-92902227 None:intergenic 55.0%
TTGGACGGCGTTGTAACCGT+CGG - chr3.1:92901798-92901817 None:intergenic 55.0%
TTGGTTCCACCCATCCTACC+AGG - chr3.1:92902249-92902268 None:intergenic 55.0%
!! ACGCCGTCCAAGTTGGTTAC+CGG + chr3.1:92901807-92901826 MS.gene68130:CDS 55.0%
!! AGAGGCTGGTGTTGGTGTGA+TGG + chr3.1:92901661-92901680 MS.gene68130:CDS 55.0%
!! ATTGGTGCCGGAACTACACC+TGG + chr3.1:92902198-92902217 MS.gene68130:CDS 55.0%
!! CGTCCAAGTTGGTTACCGGA+GGG + chr3.1:92901811-92901830 MS.gene68130:CDS 55.0%
!! GAGGCTGGTGTTGGTGTGAT+GGG + chr3.1:92901662-92901681 MS.gene68130:CDS 55.0%
GGTGCTCTTCCTGGTAAGCC+TGG + chr3.1:92902333-92902352 MS.gene68130:CDS 60.0%
GTTGTAACCGTCGGTGGCGT+CGG - chr3.1:92901789-92901808 None:intergenic 60.0%
TCTGGCACCACCATCGGCAA+GGG + chr3.1:92902001-92902020 MS.gene68130:CDS 60.0%
!! CCGTCCAAGTTGGTTACCGG+AGG + chr3.1:92901810-92901829 MS.gene68130:CDS 60.0%
CTCTGGCACCACCATCGGCA+AGG + chr3.1:92902000-92902019 MS.gene68130:CDS 65.0%
GACGGCGTTGTAACCGTCGG+TGG - chr3.1:92901795-92901814 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr3.1 gene 92901641 92902413 92901641 ID=MS.gene68130
chr3.1 mRNA 92901641 92902413 92901641 ID=MS.gene68130.t1;Parent=MS.gene68130
chr3.1 exon 92901641 92902031 92901641 ID=MS.gene68130.t1.exon1;Parent=MS.gene68130.t1
chr3.1 CDS 92901641 92902031 92901641 ID=cds.MS.gene68130.t1;Parent=MS.gene68130.t1
chr3.1 exon 92902124 92902413 92902124 ID=MS.gene68130.t1.exon2;Parent=MS.gene68130.t1
chr3.1 CDS 92902124 92902413 92902124 ID=cds.MS.gene68130.t1;Parent=MS.gene68130.t1
Gene Sequence

>MS.gene68130

ATGGTGGTGAAAATGAGCTTAGAGGCTGGTGTTGGTGTGATGGGTACCAAATTGGGAATGATGAGCTTTTTCCAACCTAACGGTGAAGTTGTTCCCGTCACTGTTGTCGGTTTCAAAGAAGGCAACATCGTCACTCAAATCAAAACCGACGCCACCGACGGTTACAACGCCGTCCAAGTTGGTTACCGGAGGGTTAGAGACCGCAAATTGACCAAACCTGAAATGGGTCATCTTGAAAAGGTTGGAGCCATTCCTATGCGACATCTTCAGGAGTTTCGTCTTCAATCCGTTGATGGTTTTGAACCTAATCAGCGCCTTGTATTTGATGAGCTTTTCAATGAAGGTGATCTTGTTGATGTCTCTGGCACCACCATCGGCAAGGGTTTTCAAGGTCTGTAACTCTCTCCCTCTTTGCATTCCCTTCTAGTTGTTGAGTTGATAATAATATCTTTCACTAATGGATGAACTTACTTACTTTGACAGGTGGTATCAAGCGTCATAACTTCAAAAGAGGACCAATGAGCCATGGTTCCAAGAGTCACAGACAACTTGGATCCATTGGTGCCGGAACTACACCTGGTCGCGTATACAAGGGAAAGAAGATGCCTGGTAGGATGGGTGGAACCAACAGGAAGACCAGAAAGCTTAAGATTGTTAAAATTGATAAAGATCTTAATGTTATCATGATCAAAGGTGCTCTTCCTGGTAAGCCTGGTAATCTTCTACGGATAGCCCCCGCTAAGATTGTTGGCAAGAACATTCCAAAGAATTAA

Protein sequence

>MS.gene68130.t1

MVVKMSLEAGVGVMGTKLGMMSFFQPNGEVVPVTVVGFKEGNIVTQIKTDATDGYNAVQVGYRRVRDRKLTKPEMGHLEKVGAIPMRHLQEFRLQSVDGFEPNQRLVFDELFNEGDLVDVSGTTIGKGFQGGIKRHNFKRGPMSHGSKSHRQLGSIGAGTTPGRVYKGKKMPGRMGGTNRKTRKLKIVKIDKDLNVIMIKGALPGKPGNLLRIAPAKIVGKNIPKN