Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene32425.t1 | XP_013466249.1 | 96.9 | 130 | 4 | 0 | 1 | 130 | 1 | 130 | 4.40E-61 | 243.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene32425.t1 | Q9ZT05 | 96.9 | 127 | 4 | 0 | 1 | 127 | 1 | 127 | 6.8e-63 | 241.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene32425.t1 | I3SQF1 | 96.9 | 130 | 4 | 0 | 1 | 130 | 1 | 130 | 3.1e-61 | 243.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049217 | MS.gene32425 | 0.814488 | 1.50E-51 | -1.69E-46 |
| MS.gene049285 | MS.gene32425 | 0.818221 | 2.19E-52 | -1.69E-46 |
| MS.gene049320 | MS.gene32425 | 0.836898 | 7.14E-57 | -1.69E-46 |
| MS.gene049351 | MS.gene32425 | 0.813148 | 2.96E-51 | -1.69E-46 |
| MS.gene049356 | MS.gene32425 | 0.816978 | 4.19E-52 | -1.69E-46 |
| MS.gene049369 | MS.gene32425 | 0.839314 | 1.71E-57 | -1.69E-46 |
| MS.gene049427 | MS.gene32425 | 0.827559 | 1.46E-54 | -1.69E-46 |
| MS.gene049609 | MS.gene32425 | 0.837271 | 5.73E-57 | -1.69E-46 |
| MS.gene049614 | MS.gene32425 | 0.831976 | 1.23E-55 | -1.69E-46 |
| MS.gene049661 | MS.gene32425 | 0.848698 | 5.19E-60 | -1.69E-46 |
| MS.gene049662 | MS.gene32425 | 0.835317 | 1.80E-56 | -1.69E-46 |
| MS.gene049760 | MS.gene32425 | 0.804455 | 2.14E-49 | -1.69E-46 |
| MS.gene049805 | MS.gene32425 | 0.861121 | 1.28E-63 | -1.69E-46 |
| MS.gene050076 | MS.gene32425 | 0.858505 | 7.87E-63 | -1.69E-46 |
| MS.gene050163 | MS.gene32425 | 0.811505 | 6.77E-51 | -1.69E-46 |
| MS.gene050333 | MS.gene32425 | 0.806633 | 7.48E-50 | -1.69E-46 |
| MS.gene050397 | MS.gene32425 | 0.823935 | 1.06E-53 | -1.69E-46 |
| MS.gene050432 | MS.gene32425 | 0.904662 | 9.71E-80 | -1.69E-46 |
| MS.gene050520 | MS.gene32425 | 0.816477 | 5.42E-52 | -1.69E-46 |
| MS.gene050593 | MS.gene32425 | 0.825466 | 4.62E-54 | -1.69E-46 |
| MS.gene050612 | MS.gene32425 | 0.802334 | 5.89E-49 | -1.69E-46 |
| MS.gene050670 | MS.gene32425 | 0.801514 | 8.69E-49 | -1.69E-46 |
| MS.gene050686 | MS.gene32425 | 0.829862 | 4.06E-55 | -1.69E-46 |
| MS.gene050699 | MS.gene32425 | 0.805287 | 1.44E-49 | -1.69E-46 |
| MS.gene050767 | MS.gene32425 | 0.892601 | 1.37E-74 | -1.69E-46 |
| MS.gene050770 | MS.gene32425 | 0.894395 | 2.57E-75 | -1.69E-46 |
| MS.gene050772 | MS.gene32425 | 0.861692 | 8.58E-64 | -1.69E-46 |
| MS.gene05101 | MS.gene32425 | 0.835088 | 2.06E-56 | -1.69E-46 |
| MS.gene05105 | MS.gene32425 | 0.850672 | 1.46E-60 | -1.69E-46 |
| MS.gene051228 | MS.gene32425 | 0.870697 | 1.20E-66 | -1.69E-46 |
| MS.gene051387 | MS.gene32425 | 0.800826 | 1.20E-48 | -1.69E-46 |
| MS.gene051471 | MS.gene32425 | 0.800726 | 1.26E-48 | -1.69E-46 |
| MS.gene051666 | MS.gene32425 | 0.819901 | 9.10E-53 | -1.69E-46 |
| MS.gene051692 | MS.gene32425 | 0.855407 | 6.44E-62 | -1.69E-46 |
| MS.gene051810 | MS.gene32425 | 0.870314 | 1.60E-66 | -1.69E-46 |
| MS.gene051858 | MS.gene32425 | 0.824842 | 6.48E-54 | -1.69E-46 |
| MS.gene052078 | MS.gene32425 | 0.835694 | 1.45E-56 | -1.69E-46 |
| MS.gene052271 | MS.gene32425 | 0.874117 | 8.68E-68 | -1.69E-46 |
| MS.gene052290 | MS.gene32425 | 0.847312 | 1.25E-59 | -1.69E-46 |
| MS.gene052310 | MS.gene32425 | 0.82944 | 5.14E-55 | -1.69E-46 |
| MS.gene052322 | MS.gene32425 | 0.811596 | 6.47E-51 | -1.69E-46 |
| MS.gene052326 | MS.gene32425 | 0.801654 | 8.13E-49 | -1.69E-46 |
| MS.gene052340 | MS.gene32425 | 0.808225 | 3.44E-50 | -1.69E-46 |
| MS.gene052379 | MS.gene32425 | 0.854343 | 1.31E-61 | -1.69E-46 |
| MS.gene052421 | MS.gene32425 | 0.840511 | 8.32E-58 | -1.69E-46 |
| MS.gene052481 | MS.gene32425 | 0.808564 | 2.91E-50 | -1.69E-46 |
| MS.gene052484 | MS.gene32425 | 0.83901 | 2.05E-57 | -1.69E-46 |
| MS.gene052485 | MS.gene32425 | 0.818121 | 2.31E-52 | -1.69E-46 |
| MS.gene052508 | MS.gene32425 | 0.808311 | 3.30E-50 | -1.69E-46 |
| MS.gene052615 | MS.gene32425 | 0.854498 | 1.18E-61 | -1.69E-46 |
| MS.gene052916 | MS.gene32425 | 0.810272 | 1.25E-50 | -1.69E-46 |
| MS.gene053009 | MS.gene32425 | 0.848127 | 7.47E-60 | -1.69E-46 |
| MS.gene053103 | MS.gene32425 | 0.842679 | 2.23E-58 | -1.69E-46 |
| MS.gene053104 | MS.gene32425 | 0.851826 | 6.89E-61 | -1.69E-46 |
| MS.gene053207 | MS.gene32425 | 0.849159 | 3.87E-60 | -1.69E-46 |
| MS.gene053209 | MS.gene32425 | 0.884732 | 1.49E-71 | -1.69E-46 |
| MS.gene053213 | MS.gene32425 | 0.847852 | 8.89E-60 | -1.69E-46 |
| MS.gene053286 | MS.gene32425 | 0.834818 | 2.41E-56 | -1.69E-46 |
| MS.gene053322 | MS.gene32425 | 0.870211 | 1.73E-66 | -1.69E-46 |
| MS.gene053384 | MS.gene32425 | 0.822241 | 2.63E-53 | -1.69E-46 |
| MS.gene053449 | MS.gene32425 | 0.850097 | 2.12E-60 | -1.69E-46 |
| MS.gene053574 | MS.gene32425 | 0.805231 | 1.48E-49 | -1.69E-46 |
| MS.gene053632 | MS.gene32425 | 0.834382 | 3.10E-56 | -1.69E-46 |
| MS.gene053813 | MS.gene32425 | 0.859262 | 4.67E-63 | -1.69E-46 |
| MS.gene053916 | MS.gene32425 | 0.846262 | 2.43E-59 | -1.69E-46 |
| MS.gene054376 | MS.gene32425 | 0.87728 | 7.13E-69 | -1.69E-46 |
| MS.gene054543 | MS.gene32425 | 0.804111 | 2.53E-49 | -1.69E-46 |
| MS.gene054600 | MS.gene32425 | 0.828887 | 7.00E-55 | -1.69E-46 |
| MS.gene054642 | MS.gene32425 | 0.850639 | 1.49E-60 | -1.69E-46 |
| MS.gene054802 | MS.gene32425 | 0.854601 | 1.10E-61 | -1.69E-46 |
| MS.gene054803 | MS.gene32425 | 0.850746 | 1.39E-60 | -1.69E-46 |
| MS.gene054804 | MS.gene32425 | 0.859046 | 5.42E-63 | -1.69E-46 |
| MS.gene05505 | MS.gene32425 | 0.864681 | 1.02E-64 | -1.69E-46 |
| MS.gene055116 | MS.gene32425 | 0.82306 | 1.70E-53 | -1.69E-46 |
| MS.gene055206 | MS.gene32425 | 0.830871 | 2.30E-55 | -1.69E-46 |
| MS.gene055254 | MS.gene32425 | 0.869501 | 2.95E-66 | -1.69E-46 |
| MS.gene055277 | MS.gene32425 | 0.862431 | 5.09E-64 | -1.69E-46 |
| MS.gene055289 | MS.gene32425 | 0.808856 | 2.52E-50 | -1.69E-46 |
| MS.gene055362 | MS.gene32425 | 0.81961 | 1.06E-52 | -1.69E-46 |
| MS.gene055397 | MS.gene32425 | 0.875008 | 4.32E-68 | -1.69E-46 |
| MS.gene055412 | MS.gene32425 | 0.827652 | 1.39E-54 | -1.69E-46 |
| MS.gene055499 | MS.gene32425 | 0.87668 | 1.15E-68 | -1.69E-46 |
| MS.gene055636 | MS.gene32425 | 0.831136 | 1.98E-55 | -1.69E-46 |
| MS.gene055638 | MS.gene32425 | 0.812413 | 4.29E-51 | -1.69E-46 |
| MS.gene055647 | MS.gene32425 | 0.840569 | 8.04E-58 | -1.69E-46 |
| MS.gene055722 | MS.gene32425 | 0.866093 | 3.67E-65 | -1.69E-46 |
| MS.gene05592 | MS.gene32425 | 0.92007 | 2.04E-87 | -1.69E-46 |
| MS.gene055941 | MS.gene32425 | 0.82101 | 5.07E-53 | -1.69E-46 |
| MS.gene056191 | MS.gene32425 | 0.830281 | 3.21E-55 | -1.69E-46 |
| MS.gene056374 | MS.gene32425 | 0.863713 | 2.05E-64 | -1.69E-46 |
| MS.gene056407 | MS.gene32425 | 0.802953 | 4.39E-49 | -1.69E-46 |
| MS.gene056410 | MS.gene32425 | 0.819628 | 1.05E-52 | -1.69E-46 |
| MS.gene056422 | MS.gene32425 | 0.807987 | 3.86E-50 | -1.69E-46 |
| MS.gene056487 | MS.gene32425 | 0.808518 | 2.98E-50 | -1.69E-46 |
| MS.gene056511 | MS.gene32425 | 0.811892 | 5.58E-51 | -1.69E-46 |
| MS.gene056609 | MS.gene32425 | 0.876123 | 1.79E-68 | -1.69E-46 |
| MS.gene056657 | MS.gene32425 | 0.829467 | 5.07E-55 | -1.69E-46 |
| MS.gene056897 | MS.gene32425 | 0.825258 | 5.17E-54 | -1.69E-46 |
| MS.gene057011 | MS.gene32425 | 0.832654 | 8.36E-56 | -1.69E-46 |
| MS.gene057076 | MS.gene32425 | 0.829941 | 3.88E-55 | -1.69E-46 |
| MS.gene057123 | MS.gene32425 | 0.805154 | 1.53E-49 | -1.69E-46 |
| MS.gene057124 | MS.gene32425 | 0.834897 | 2.30E-56 | -1.69E-46 |
| MS.gene057129 | MS.gene32425 | 0.904433 | 1.23E-79 | -1.69E-46 |
| MS.gene057146 | MS.gene32425 | 0.811366 | 7.26E-51 | -1.69E-46 |
| MS.gene057295 | MS.gene32425 | 0.82495 | 6.11E-54 | -1.69E-46 |
| MS.gene057331 | MS.gene32425 | 0.869279 | 3.49E-66 | -1.69E-46 |
| MS.gene05733 | MS.gene32425 | 0.803709 | 3.06E-49 | -1.69E-46 |
| MS.gene05735 | MS.gene32425 | 0.856022 | 4.26E-62 | -1.69E-46 |
| MS.gene057416 | MS.gene32425 | 0.850118 | 2.09E-60 | -1.69E-46 |
| MS.gene057450 | MS.gene32425 | 0.801771 | 7.69E-49 | -1.69E-46 |
| MS.gene057926 | MS.gene32425 | 0.838403 | 2.94E-57 | -1.69E-46 |
| MS.gene057927 | MS.gene32425 | 0.801893 | 7.26E-49 | -1.69E-46 |
| MS.gene058006 | MS.gene32425 | 0.872628 | 2.75E-67 | -1.69E-46 |
| MS.gene058019 | MS.gene32425 | 0.826181 | 3.12E-54 | -1.69E-46 |
| MS.gene058130 | MS.gene32425 | 0.812518 | 4.07E-51 | -1.69E-46 |
| MS.gene058293 | MS.gene32425 | 0.872582 | 2.85E-67 | -1.69E-46 |
| MS.gene058323 | MS.gene32425 | 0.855601 | 5.65E-62 | -1.69E-46 |
| MS.gene058365 | MS.gene32425 | 0.813823 | 2.11E-51 | -1.69E-46 |
| MS.gene058419 | MS.gene32425 | 0.833208 | 6.09E-56 | -1.69E-46 |
| MS.gene058660 | MS.gene32425 | 0.84292 | 1.93E-58 | -1.69E-46 |
| MS.gene058723 | MS.gene32425 | 0.842172 | 3.04E-58 | -1.69E-46 |
| MS.gene058787 | MS.gene32425 | 0.800235 | 1.59E-48 | -1.69E-46 |
| MS.gene058903 | MS.gene32425 | 0.800481 | 1.41E-48 | -1.69E-46 |
| MS.gene058928 | MS.gene32425 | 0.822939 | 1.81E-53 | -1.69E-46 |
| MS.gene059029 | MS.gene32425 | 0.834852 | 2.36E-56 | -1.69E-46 |
| MS.gene059326 | MS.gene32425 | 0.823952 | 1.05E-53 | -1.69E-46 |
| MS.gene059366 | MS.gene32425 | 0.816959 | 4.23E-52 | -1.69E-46 |
| MS.gene059536 | MS.gene32425 | 0.83071 | 2.52E-55 | -1.69E-46 |
| MS.gene059676 | MS.gene32425 | 0.827698 | 1.35E-54 | -1.69E-46 |
| MS.gene059677 | MS.gene32425 | 0.808978 | 2.37E-50 | -1.69E-46 |
| MS.gene059688 | MS.gene32425 | 0.862635 | 4.41E-64 | -1.69E-46 |
| MS.gene059692 | MS.gene32425 | 0.879318 | 1.37E-69 | -1.69E-46 |
| MS.gene059695 | MS.gene32425 | 0.873118 | 1.88E-67 | -1.69E-46 |
| MS.gene059711 | MS.gene32425 | 0.832667 | 8.30E-56 | -1.69E-46 |
| MS.gene059718 | MS.gene32425 | 0.817549 | 3.11E-52 | -1.69E-46 |
| MS.gene059858 | MS.gene32425 | 0.80325 | 3.81E-49 | -1.69E-46 |
| MS.gene059894 | MS.gene32425 | 0.821167 | 4.66E-53 | -1.69E-46 |
| MS.gene059902 | MS.gene32425 | 0.821387 | 4.15E-53 | -1.69E-46 |
| MS.gene059960 | MS.gene32425 | 0.870285 | 1.64E-66 | -1.69E-46 |
| MS.gene059974 | MS.gene32425 | 0.833474 | 5.22E-56 | -1.69E-46 |
| MS.gene060005 | MS.gene32425 | 0.809408 | 1.92E-50 | -1.69E-46 |
| MS.gene060193 | MS.gene32425 | 0.836104 | 1.14E-56 | -1.69E-46 |
| MS.gene060356 | MS.gene32425 | 0.804193 | 2.43E-49 | -1.69E-46 |
| MS.gene06037 | MS.gene32425 | 0.84453 | 7.14E-59 | -1.69E-46 |
| MS.gene060403 | MS.gene32425 | 0.803706 | 3.07E-49 | -1.69E-46 |
| MS.gene060804 | MS.gene32425 | 0.868738 | 5.22E-66 | -1.69E-46 |
| MS.gene060818 | MS.gene32425 | 0.866339 | 3.06E-65 | -1.69E-46 |
| MS.gene060835 | MS.gene32425 | 0.823443 | 1.38E-53 | -1.69E-46 |
| MS.gene060837 | MS.gene32425 | 0.832429 | 9.50E-56 | -1.69E-46 |
| MS.gene06092 | MS.gene32425 | 0.81319 | 2.90E-51 | -1.69E-46 |
| MS.gene061041 | MS.gene32425 | 0.85408 | 1.56E-61 | -1.69E-46 |
| MS.gene061049 | MS.gene32425 | 0.817139 | 3.85E-52 | -1.69E-46 |
| MS.gene061051 | MS.gene32425 | -0.840958 | 6.35E-58 | -1.69E-46 |
| MS.gene061134 | MS.gene32425 | 0.80278 | 4.77E-49 | -1.69E-46 |
| MS.gene061150 | MS.gene32425 | 0.803928 | 2.76E-49 | -1.69E-46 |
| MS.gene061200 | MS.gene32425 | 0.876083 | 1.85E-68 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene32425.t1 | MTR_1g026820 | 96.923 | 130 | 4 | 0 | 1 | 130 | 1 | 130 | 3.76e-87 | 249 |
| MS.gene32425.t1 | MTR_1g026820 | 97.959 | 98 | 2 | 0 | 33 | 130 | 1 | 98 | 3.99e-64 | 190 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene32425.t1 | AT1G30380 | 81.679 | 131 | 22 | 2 | 1 | 130 | 1 | 130 | 1.29e-72 | 213 |
Find 52 sgRNAs with CRISPR-Local
Find 68 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATTGGTGTTGGTGTTGTTCT+TGG | 0.264103 | 1.4:-12665703 | MS.gene32425:CDS |
| GCGCAAAGGCAAGGGTGGTT+TGG | 0.284320 | 1.4:-12666248 | MS.gene32425:CDS |
| ATCTTCCAGCAAACAACATT+AGG | 0.298751 | 1.4:+12665863 | None:intergenic |
| CGCAAAGGCAAGGGTGGTTT+GGG | 0.299534 | 1.4:-12666247 | MS.gene32425:CDS |
| TTGCCTTTGCGCCTTGTCAT+AGG | 0.312155 | 1.4:+12666259 | None:intergenic |
| GTTCACCCTTGCTGATACTT+TGG | 0.359682 | 1.4:-12665749 | MS.gene32425:CDS |
| TTGCAGACTGGTGACCCAGC+TGG | 0.370604 | 1.4:-12665772 | MS.gene32425:CDS |
| ACCTTCTCCTATCCAGGGTT+TGG | 0.377236 | 1.4:-12666525 | MS.gene32425:intron |
| ATGTTGTTTGCTGGAAGATT+TGG | 0.379579 | 1.4:-12665859 | MS.gene32425:CDS |
| TTGAAATCATACCACATTAG+TGG | 0.382365 | 1.4:+12666198 | None:intergenic |
| GGAGTTCGTTGTGACTTCAT+TGG | 0.392047 | 1.4:-12666226 | MS.gene32425:CDS |
| TCTTCCAGCAAACAACATTA+GGG | 0.393888 | 1.4:+12665864 | None:intergenic |
| ACAACCCTAATGTTGTTTGC+TGG | 0.398888 | 1.4:-12665868 | MS.gene32425:CDS |
| TGCAGACTGGTGACCCAGCT+GGG | 0.430536 | 1.4:-12665771 | MS.gene32425:CDS |
| CTTTGCGCCTTGTCATAGGT+TGG | 0.430581 | 1.4:+12666263 | None:intergenic |
| ACTCAAACCTTCTCCTATCC+AGG | 0.437202 | 1.4:-12666531 | MS.gene32425:CDS |
| TCAGCAAGGGTGAACCCAGC+TGG | 0.438597 | 1.4:+12665757 | None:intergenic |
| GATTGGCTCCCTCAGCAAAC+AGG | 0.449260 | 1.4:-12665837 | MS.gene32425:CDS |
| TTTGCGCCTTGTCATAGGTT+GGG | 0.452938 | 1.4:+12666264 | None:intergenic |
| ACTTTGGCCTGTGGGACTGT+TGG | 0.461540 | 1.4:-12665733 | MS.gene32425:CDS |
| ACAACTTTGCCTCAATTCAC+TGG | 0.462189 | 1.4:-12666566 | MS.gene32425:CDS |
| AGCAACAGCAGGACTAAAGC+TGG | 0.487183 | 1.4:-12665812 | MS.gene32425:CDS |
| GTTGCTTTCCTGTTTGCTGA+GGG | 0.491197 | 1.4:+12665829 | None:intergenic |
| GTTTGCTGGAAGATTTGGAT+TGG | 0.494741 | 1.4:-12665854 | MS.gene32425:CDS |
| TTGCTGATACTTTGGCCTGT+GGG | 0.511025 | 1.4:-12665741 | MS.gene32425:CDS |
| CTTGCTGATACTTTGGCCTG+TGG | 0.513317 | 1.4:-12665742 | MS.gene32425:CDS |
| TATGACAAGGCGCAAAGGCA+AGG | 0.517020 | 1.4:-12666257 | MS.gene32425:CDS |
| GGGACTGTTGGACACATCAT+TGG | 0.518734 | 1.4:-12665721 | MS.gene32425:CDS |
| GTTCTTGGTCTTAAGAACAT+TGG | 0.536798 | 1.4:-12665688 | MS.gene32425:CDS |
| AGGGACTCAGGCTTGCAGAC+TGG | 0.536809 | 1.4:-12665784 | MS.gene32425:CDS |
| CAGGACTAAAGCTGGAGACA+AGG | 0.539368 | 1.4:-12665804 | MS.gene32425:CDS |
| TGTTGCTTTCCTGTTTGCTG+AGG | 0.553786 | 1.4:+12665828 | None:intergenic |
| ACCAAACCCTGGATAGGAGA+AGG | 0.553964 | 1.4:+12666524 | None:intergenic |
| GTTGGACACATCATTGGTGT+TGG | 0.557734 | 1.4:-12665715 | MS.gene32425:CDS |
| AAGCTGGAGACAAGGGACTC+AGG | 0.559360 | 1.4:-12665796 | MS.gene32425:CDS |
| GATGTGTCCAACAGTCCCAC+AGG | 0.566507 | 1.4:+12665726 | None:intergenic |
| CAGCAAGGGTGAACCCAGCT+GGG | 0.567280 | 1.4:+12665758 | None:intergenic |
| ATGACAAGGCGCAAAGGCAA+GGG | 0.568554 | 1.4:-12666256 | MS.gene32425:CDS |
| ATAGGAGAAGGTTTGAGTTG+TGG | 0.576762 | 1.4:+12666536 | None:intergenic |
| TTAATTACCAAACCCTGGAT+AGG | 0.584959 | 1.4:+12666518 | None:intergenic |
| CATTGGTTCATCCACTAATG+TGG | 0.586749 | 1.4:-12666209 | MS.gene32425:intron |
| AGGACTAAAGCTGGAGACAA+GGG | 0.595838 | 1.4:-12665803 | MS.gene32425:CDS |
| CTCAAACCTTCTCCTATCCA+GGG | 0.595941 | 1.4:-12666530 | MS.gene32425:CDS |
| CACAGGCCAAAGTATCAGCA+AGG | 0.601389 | 1.4:+12665743 | None:intergenic |
| AGTGGTTAATTACCAAACCC+TGG | 0.602633 | 1.4:+12666513 | None:intergenic |
| GGTCTAAGACCAGTGAATTG+AGG | 0.606924 | 1.4:+12666557 | None:intergenic |
| GCAAACAGGAAAGCAACAGC+AGG | 0.611764 | 1.4:-12665823 | MS.gene32425:CDS |
| CAACCTATGACAAGGCGCAA+AGG | 0.611923 | 1.4:-12666262 | MS.gene32425:CDS |
| ACAAGGCGCAAAGGCAAGGG+TGG | 0.641021 | 1.4:-12666253 | MS.gene32425:CDS |
| ACAGGCCAAAGTATCAGCAA+GGG | 0.672894 | 1.4:+12665744 | None:intergenic |
| TTGCTGCCCAACCTATGACA+AGG | 0.674324 | 1.4:-12666270 | MS.gene32425:CDS |
| ACATGTTGGACAGATTATGG+TGG | 0.710941 | 1.4:-12665896 | MS.gene32425:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTTAAATATATATAGTATGA+AGG | + | chr1.4:12665919-12665938 | None:intergenic | 10.0% |
| !!! | TTTAAGTTTTATAATTCATA+AGG | + | chr1.4:12665883-12665902 | None:intergenic | 10.0% |
| !! | ATTCATATTTGTAAATTGTA+TGG | + | chr1.4:12665851-12665870 | None:intergenic | 15.0% |
| !!! | TGTTTATTTTTTTTATGTGA+AGG | - | chr1.4:12665969-12665988 | MS.gene32425:intron | 15.0% |
| !! | AAAACTATACATATCATGAT+TGG | + | chr1.4:12666245-12666264 | None:intergenic | 20.0% |
| !! | ATGAATAAAAACATTGGAAT+TGG | + | chr1.4:12666099-12666118 | None:intergenic | 20.0% |
| !! | TATTCTATATACTTACTTCT+TGG | - | chr1.4:12665824-12665843 | MS.gene32425:CDS | 20.0% |
| ! | AAGTTACAAATTCTAACAAG+TGG | + | chr1.4:12666314-12666333 | None:intergenic | 25.0% |
| AAATGATGACATGAAAAGAG+TGG | + | chr1.4:12665770-12665789 | None:intergenic | 30.0% | |
| AAGCATAGTGTCACAATAAT+TGG | + | chr1.4:12666273-12666292 | None:intergenic | 30.0% | |
| CGCACTATGAATAAAAACAT+TGG | + | chr1.4:12666105-12666124 | None:intergenic | 30.0% | |
| TAACGAGTTTCGCAAAATTA+CGG | + | chr1.4:12666220-12666239 | None:intergenic | 30.0% | |
| TTGAAATCATACCACATTAG+TGG | + | chr1.4:12666067-12666086 | None:intergenic | 30.0% | |
| ! | TATGATTGACTTTGACATGT+TGG | - | chr1.4:12666352-12666371 | MS.gene32425:intron | 30.0% |
| ATCTTCCAGCAAACAACATT+AGG | + | chr1.4:12666402-12666421 | None:intergenic | 35.0% | |
| TCTTCCAGCAAACAACATTA+GGG | + | chr1.4:12666401-12666420 | None:intergenic | 35.0% | |
| TTAATTACCAAACCCTGGAT+AGG | + | chr1.4:12665747-12665766 | None:intergenic | 35.0% | |
| TTGACATGTTGGACAGATTA+TGG | - | chr1.4:12666363-12666382 | MS.gene32425:intron | 35.0% | |
| ! | ATGTTGTTTGCTGGAAGATT+TGG | - | chr1.4:12666403-12666422 | MS.gene32425:intron | 35.0% |
| ! | TTTTATTCATAGTGCGTGTC+TGG | - | chr1.4:12666109-12666128 | MS.gene32425:intron | 35.0% |
| !! | GTTCTTGGTCTTAAGAACAT+TGG | - | chr1.4:12666574-12666593 | MS.gene32425:CDS | 35.0% |
| ACAACTTTGCCTCAATTCAC+TGG | - | chr1.4:12665696-12665715 | MS.gene32425:CDS | 40.0% | |
| ACATGTTGGACAGATTATGG+TGG | - | chr1.4:12666366-12666385 | MS.gene32425:intron | 40.0% | |
| AGTGGTTAATTACCAAACCC+TGG | + | chr1.4:12665752-12665771 | None:intergenic | 40.0% | |
| ! | ACAACCCTAATGTTGTTTGC+TGG | - | chr1.4:12666394-12666413 | MS.gene32425:intron | 40.0% |
| ! | GTTTGCTGGAAGATTTGGAT+TGG | - | chr1.4:12666408-12666427 | MS.gene32425:intron | 40.0% |
| !! | ATAGGAGAAGGTTTGAGTTG+TGG | + | chr1.4:12665729-12665748 | None:intergenic | 40.0% |
| !! | ATTGGTGTTGGTGTTGTTCT+TGG | - | chr1.4:12666559-12666578 | MS.gene32425:CDS | 40.0% |
| !! | CATTGGTTCATCCACTAATG+TGG | - | chr1.4:12666053-12666072 | MS.gene32425:intron | 40.0% |
| ACTCAAACCTTCTCCTATCC+AGG | - | chr1.4:12665731-12665750 | MS.gene32425:CDS | 45.0% | |
| AGGACTAAAGCTGGAGACAA+GGG | - | chr1.4:12666459-12666478 | MS.gene32425:intron | 45.0% | |
| CTCAAACCTTCTCCTATCCA+GGG | - | chr1.4:12665732-12665751 | MS.gene32425:CDS | 45.0% | |
| GGAGTTCGTTGTGACTTCAT+TGG | - | chr1.4:12666036-12666055 | MS.gene32425:intron | 45.0% | |
| GTTCACCCTTGCTGATACTT+TGG | - | chr1.4:12666513-12666532 | MS.gene32425:intron | 45.0% | |
| GTTGGACACATCATTGGTGT+TGG | - | chr1.4:12666547-12666566 | MS.gene32425:CDS | 45.0% | |
| TTGCTGATACTTTGGCCTGT+GGG | - | chr1.4:12666521-12666540 | MS.gene32425:intron | 45.0% | |
| TTTGCGCCTTGTCATAGGTT+GGG | + | chr1.4:12666001-12666020 | None:intergenic | 45.0% | |
| ! | ACAGGCCAAAGTATCAGCAA+GGG | + | chr1.4:12666521-12666540 | None:intergenic | 45.0% |
| ! | GGTCTAAGACCAGTGAATTG+AGG | + | chr1.4:12665708-12665727 | None:intergenic | 45.0% |
| ! | GTTGCTTTCCTGTTTGCTGA+GGG | + | chr1.4:12666436-12666455 | None:intergenic | 45.0% |
| !! | TGTTGCTTTCCTGTTTGCTG+AGG | + | chr1.4:12666437-12666456 | None:intergenic | 45.0% |
| !!! | TACTATATATATTTAAATTT+TGG | - | chr1.4:12665922-12665941 | MS.gene32425:intron | 5.0% |
| ACCAAACCCTGGATAGGAGA+AGG | + | chr1.4:12665741-12665760 | None:intergenic | 50.0% | |
| ACCTTCTCCTATCCAGGGTT+TGG | - | chr1.4:12665737-12665756 | MS.gene32425:CDS | 50.0% | |
| AGCAACAGCAGGACTAAAGC+TGG | - | chr1.4:12666450-12666469 | MS.gene32425:intron | 50.0% | |
| ATGACAAGGCGCAAAGGCAA+GGG | - | chr1.4:12666006-12666025 | MS.gene32425:intron | 50.0% | |
| CAACCTATGACAAGGCGCAA+AGG | - | chr1.4:12666000-12666019 | MS.gene32425:intron | 50.0% | |
| CAGGACTAAAGCTGGAGACA+AGG | - | chr1.4:12666458-12666477 | MS.gene32425:intron | 50.0% | |
| CTTGCTGATACTTTGGCCTG+TGG | - | chr1.4:12666520-12666539 | MS.gene32425:intron | 50.0% | |
| CTTTGCGCCTTGTCATAGGT+TGG | + | chr1.4:12666002-12666021 | None:intergenic | 50.0% | |
| GCAAACAGGAAAGCAACAGC+AGG | - | chr1.4:12666439-12666458 | MS.gene32425:intron | 50.0% | |
| GGGACTGTTGGACACATCAT+TGG | - | chr1.4:12666541-12666560 | MS.gene32425:CDS | 50.0% | |
| TATGACAAGGCGCAAAGGCA+AGG | - | chr1.4:12666005-12666024 | MS.gene32425:intron | 50.0% | |
| TTGCCTTTGCGCCTTGTCAT+AGG | + | chr1.4:12666006-12666025 | None:intergenic | 50.0% | |
| TTGCTGCCCAACCTATGACA+AGG | - | chr1.4:12665992-12666011 | MS.gene32425:intron | 50.0% | |
| ! | CACAGGCCAAAGTATCAGCA+AGG | + | chr1.4:12666522-12666541 | None:intergenic | 50.0% |
| AAGCTGGAGACAAGGGACTC+AGG | - | chr1.4:12666466-12666485 | MS.gene32425:intron | 55.0% | |
| ACTTTGGCCTGTGGGACTGT+TGG | - | chr1.4:12666529-12666548 | MS.gene32425:CDS | 55.0% | |
| CGCAAAGGCAAGGGTGGTTT+GGG | - | chr1.4:12666015-12666034 | MS.gene32425:intron | 55.0% | |
| GATGTGTCCAACAGTCCCAC+AGG | + | chr1.4:12666539-12666558 | None:intergenic | 55.0% | |
| ! | GATTGGCTCCCTCAGCAAAC+AGG | - | chr1.4:12666425-12666444 | MS.gene32425:intron | 55.0% |
| ACAAGGCGCAAAGGCAAGGG+TGG | - | chr1.4:12666009-12666028 | MS.gene32425:intron | 60.0% | |
| AGGGACTCAGGCTTGCAGAC+TGG | - | chr1.4:12666478-12666497 | MS.gene32425:intron | 60.0% | |
| CAGCAAGGGTGAACCCAGCT+GGG | + | chr1.4:12666507-12666526 | None:intergenic | 60.0% | |
| GCGCAAAGGCAAGGGTGGTT+TGG | - | chr1.4:12666014-12666033 | MS.gene32425:intron | 60.0% | |
| TCAGCAAGGGTGAACCCAGC+TGG | + | chr1.4:12666508-12666527 | None:intergenic | 60.0% | |
| TGCAGACTGGTGACCCAGCT+GGG | - | chr1.4:12666491-12666510 | MS.gene32425:intron | 60.0% | |
| TTGCAGACTGGTGACCCAGC+TGG | - | chr1.4:12666490-12666509 | MS.gene32425:intron | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.4 | gene | 12665678 | 12666606 | 12665678 | ID=MS.gene32425 |
| chr1.4 | mRNA | 12665678 | 12666606 | 12665678 | ID=MS.gene32425.t1;Parent=MS.gene32425 |
| chr1.4 | exon | 12666526 | 12666606 | 12666526 | ID=MS.gene32425.t1.exon1;Parent=MS.gene32425.t1 |
| chr1.4 | CDS | 12666526 | 12666606 | 12666526 | ID=cds.MS.gene32425.t1;Parent=MS.gene32425.t1 |
| chr1.4 | exon | 12666210 | 12666293 | 12666210 | ID=MS.gene32425.t1.exon2;Parent=MS.gene32425.t1 |
| chr1.4 | CDS | 12666210 | 12666293 | 12666210 | ID=cds.MS.gene32425.t1;Parent=MS.gene32425.t1 |
| chr1.4 | exon | 12665678 | 12665905 | 12665678 | ID=MS.gene32425.t1.exon3;Parent=MS.gene32425.t1 |
| chr1.4 | CDS | 12665678 | 12665905 | 12665678 | ID=cds.MS.gene32425.t1;Parent=MS.gene32425.t1 |
| Gene Sequence |
| Protein sequence |
