Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene55694.t1 | XP_013460500.1 | 94.8 | 192 | 6 | 2 | 1 | 188 | 23 | 214 | 4.10E-92 | 347.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene55694.t1 | O80363 | 79.1 | 196 | 33 | 4 | 1 | 188 | 10 | 205 | 6.4e-78 | 291.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene55694.t1 | A0A072UXJ8 | 94.8 | 192 | 6 | 2 | 1 | 188 | 23 | 214 | 2.9e-92 | 347.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049171 | MS.gene55694 | 0.846422 | 2.19E-59 | -1.69E-46 |
| MS.gene049173 | MS.gene55694 | 0.807563 | 4.76E-50 | -1.69E-46 |
| MS.gene049217 | MS.gene55694 | 0.869568 | 2.81E-66 | -1.69E-46 |
| MS.gene049222 | MS.gene55694 | 0.851451 | 8.80E-61 | -1.69E-46 |
| MS.gene049232 | MS.gene55694 | 0.80504 | 1.62E-49 | -1.69E-46 |
| MS.gene049285 | MS.gene55694 | 0.902382 | 1.03E-78 | -1.69E-46 |
| MS.gene049320 | MS.gene55694 | 0.885193 | 1.00E-71 | -1.69E-46 |
| MS.gene049627 | MS.gene55694 | 0.823536 | 1.31E-53 | -1.69E-46 |
| MS.gene049633 | MS.gene55694 | 0.807579 | 4.72E-50 | -1.69E-46 |
| MS.gene049805 | MS.gene55694 | 0.80149 | 8.79E-49 | -1.69E-46 |
| MS.gene05010 | MS.gene55694 | 0.842849 | 2.01E-58 | -1.69E-46 |
| MS.gene050163 | MS.gene55694 | 0.914417 | 1.96E-84 | -1.69E-46 |
| MS.gene050165 | MS.gene55694 | 0.916926 | 9.88E-86 | -1.69E-46 |
| MS.gene050333 | MS.gene55694 | 0.910014 | 3.01E-82 | -1.69E-46 |
| MS.gene050444 | MS.gene55694 | 0.817932 | 2.55E-52 | -1.69E-46 |
| MS.gene050504 | MS.gene55694 | 0.888267 | 6.87E-73 | -1.69E-46 |
| MS.gene050518 | MS.gene55694 | 0.901571 | 2.34E-78 | -1.69E-46 |
| MS.gene050577 | MS.gene55694 | 0.809517 | 1.82E-50 | -1.69E-46 |
| MS.gene050612 | MS.gene55694 | 0.8936 | 5.41E-75 | -1.69E-46 |
| MS.gene050675 | MS.gene55694 | 0.856969 | 2.25E-62 | -1.69E-46 |
| MS.gene050699 | MS.gene55694 | 0.852709 | 3.86E-61 | -1.69E-46 |
| MS.gene050767 | MS.gene55694 | 0.845723 | 3.40E-59 | -1.69E-46 |
| MS.gene050770 | MS.gene55694 | 0.847501 | 1.11E-59 | -1.69E-46 |
| MS.gene050772 | MS.gene55694 | 0.851533 | 8.34E-61 | -1.69E-46 |
| MS.gene05079 | MS.gene55694 | -0.803685 | 3.10E-49 | -1.69E-46 |
| MS.gene05080 | MS.gene55694 | 0.810597 | 1.07E-50 | -1.69E-46 |
| MS.gene050868 | MS.gene55694 | 0.818117 | 2.32E-52 | -1.69E-46 |
| MS.gene050871 | MS.gene55694 | 0.81343 | 2.57E-51 | -1.69E-46 |
| MS.gene050876 | MS.gene55694 | 0.850476 | 1.66E-60 | -1.69E-46 |
| MS.gene05087 | MS.gene55694 | 0.800541 | 1.37E-48 | -1.69E-46 |
| MS.gene050986 | MS.gene55694 | 0.852475 | 4.51E-61 | -1.69E-46 |
| MS.gene05100 | MS.gene55694 | 0.858344 | 8.79E-63 | -1.69E-46 |
| MS.gene051228 | MS.gene55694 | 0.857821 | 1.26E-62 | -1.69E-46 |
| MS.gene051317 | MS.gene55694 | 0.829803 | 4.20E-55 | -1.69E-46 |
| MS.gene051366 | MS.gene55694 | -0.802861 | 4.59E-49 | -1.69E-46 |
| MS.gene051434 | MS.gene55694 | 0.844091 | 9.37E-59 | -1.69E-46 |
| MS.gene051551 | MS.gene55694 | 0.807944 | 3.95E-50 | -1.69E-46 |
| MS.gene051666 | MS.gene55694 | 0.822697 | 2.06E-53 | -1.69E-46 |
| MS.gene051694 | MS.gene55694 | 0.82743 | 1.57E-54 | -1.69E-46 |
| MS.gene051810 | MS.gene55694 | 0.877852 | 4.51E-69 | -1.69E-46 |
| MS.gene051858 | MS.gene55694 | 0.83773 | 4.37E-57 | -1.69E-46 |
| MS.gene051949 | MS.gene55694 | 0.89008 | 1.36E-73 | -1.69E-46 |
| MS.gene052271 | MS.gene55694 | 0.867108 | 1.74E-65 | -1.69E-46 |
| MS.gene052379 | MS.gene55694 | 0.857569 | 1.49E-62 | -1.69E-46 |
| MS.gene052506 | MS.gene55694 | 0.800202 | 1.61E-48 | -1.69E-46 |
| MS.gene052822 | MS.gene55694 | 0.825693 | 4.08E-54 | -1.69E-46 |
| MS.gene052870 | MS.gene55694 | 0.814817 | 1.27E-51 | -1.69E-46 |
| MS.gene052872 | MS.gene55694 | 0.822956 | 1.79E-53 | -1.69E-46 |
| MS.gene052916 | MS.gene55694 | 0.864529 | 1.14E-64 | -1.69E-46 |
| MS.gene053009 | MS.gene55694 | 0.853956 | 1.70E-61 | -1.69E-46 |
| MS.gene053207 | MS.gene55694 | 0.801472 | 8.86E-49 | -1.69E-46 |
| MS.gene053213 | MS.gene55694 | 0.853116 | 2.96E-61 | -1.69E-46 |
| MS.gene053284 | MS.gene55694 | 0.844763 | 6.18E-59 | -1.69E-46 |
| MS.gene053285 | MS.gene55694 | 0.83907 | 1.97E-57 | -1.69E-46 |
| MS.gene053286 | MS.gene55694 | 0.853013 | 3.16E-61 | -1.69E-46 |
| MS.gene053322 | MS.gene55694 | 0.848655 | 5.34E-60 | -1.69E-46 |
| MS.gene053384 | MS.gene55694 | 0.8001 | 1.69E-48 | -1.69E-46 |
| MS.gene053561 | MS.gene55694 | -0.819515 | 1.12E-52 | -1.69E-46 |
| MS.gene053632 | MS.gene55694 | 0.834515 | 2.87E-56 | -1.69E-46 |
| MS.gene053824 | MS.gene55694 | 0.815394 | 9.45E-52 | -1.69E-46 |
| MS.gene053916 | MS.gene55694 | 0.843604 | 1.26E-58 | -1.69E-46 |
| MS.gene054374 | MS.gene55694 | 0.839788 | 1.28E-57 | -1.69E-46 |
| MS.gene054376 | MS.gene55694 | 0.808699 | 2.72E-50 | -1.69E-46 |
| MS.gene054543 | MS.gene55694 | 0.800685 | 1.28E-48 | -1.69E-46 |
| MS.gene054600 | MS.gene55694 | 0.830855 | 2.32E-55 | -1.69E-46 |
| MS.gene054642 | MS.gene55694 | 0.904268 | 1.47E-79 | -1.69E-46 |
| MS.gene054802 | MS.gene55694 | 0.822643 | 2.12E-53 | -1.69E-46 |
| MS.gene054803 | MS.gene55694 | 0.819915 | 9.04E-53 | -1.69E-46 |
| MS.gene054804 | MS.gene55694 | 0.825711 | 4.04E-54 | -1.69E-46 |
| MS.gene054954 | MS.gene55694 | 0.880397 | 5.68E-70 | -1.69E-46 |
| MS.gene055154 | MS.gene55694 | -0.804558 | 2.04E-49 | -1.69E-46 |
| MS.gene055206 | MS.gene55694 | 0.812354 | 4.42E-51 | -1.69E-46 |
| MS.gene055224 | MS.gene55694 | 0.806733 | 7.13E-50 | -1.69E-46 |
| MS.gene055362 | MS.gene55694 | 0.870976 | 9.71E-67 | -1.69E-46 |
| MS.gene055397 | MS.gene55694 | 0.876141 | 1.77E-68 | -1.69E-46 |
| MS.gene055499 | MS.gene55694 | 0.852913 | 3.38E-61 | -1.69E-46 |
| MS.gene055502 | MS.gene55694 | 0.856997 | 2.20E-62 | -1.69E-46 |
| MS.gene055577 | MS.gene55694 | 0.864429 | 1.22E-64 | -1.69E-46 |
| MS.gene055634 | MS.gene55694 | 0.860397 | 2.13E-63 | -1.69E-46 |
| MS.gene055636 | MS.gene55694 | 0.860055 | 2.70E-63 | -1.69E-46 |
| MS.gene055638 | MS.gene55694 | 0.880796 | 4.09E-70 | -1.69E-46 |
| MS.gene055647 | MS.gene55694 | 0.819464 | 1.15E-52 | -1.69E-46 |
| MS.gene055722 | MS.gene55694 | 0.816395 | 5.65E-52 | -1.69E-46 |
| MS.gene055748 | MS.gene55694 | 0.856321 | 3.48E-62 | -1.69E-46 |
| MS.gene055750 | MS.gene55694 | 0.840131 | 1.05E-57 | -1.69E-46 |
| MS.gene055751 | MS.gene55694 | 0.865899 | 4.22E-65 | -1.69E-46 |
| MS.gene055776 | MS.gene55694 | 0.83039 | 3.02E-55 | -1.69E-46 |
| MS.gene05592 | MS.gene55694 | 0.850328 | 1.82E-60 | -1.69E-46 |
| MS.gene055941 | MS.gene55694 | 0.82871 | 7.73E-55 | -1.69E-46 |
| MS.gene055956 | MS.gene55694 | -0.835216 | 1.91E-56 | -1.69E-46 |
| MS.gene056083 | MS.gene55694 | 0.824276 | 8.81E-54 | -1.69E-46 |
| MS.gene056374 | MS.gene55694 | 0.857942 | 1.16E-62 | -1.69E-46 |
| MS.gene056409 | MS.gene55694 | 0.812914 | 3.34E-51 | -1.69E-46 |
| MS.gene056504 | MS.gene55694 | 0.815066 | 1.12E-51 | -1.69E-46 |
| MS.gene056510 | MS.gene55694 | 0.845656 | 3.54E-59 | -1.69E-46 |
| MS.gene056549 | MS.gene55694 | 0.837666 | 4.54E-57 | -1.69E-46 |
| MS.gene056657 | MS.gene55694 | 0.882999 | 6.50E-71 | -1.69E-46 |
| MS.gene056692 | MS.gene55694 | 0.847523 | 1.10E-59 | -1.69E-46 |
| MS.gene056898 | MS.gene55694 | 0.812495 | 4.12E-51 | -1.69E-46 |
| MS.gene057129 | MS.gene55694 | 0.887312 | 1.59E-72 | -1.69E-46 |
| MS.gene057246 | MS.gene55694 | 0.85737 | 1.71E-62 | -1.69E-46 |
| MS.gene057331 | MS.gene55694 | 0.82568 | 4.11E-54 | -1.69E-46 |
| MS.gene057416 | MS.gene55694 | 0.84116 | 5.62E-58 | -1.69E-46 |
| MS.gene057926 | MS.gene55694 | 0.830916 | 2.24E-55 | -1.69E-46 |
| MS.gene057986 | MS.gene55694 | 0.840722 | 7.33E-58 | -1.69E-46 |
| MS.gene058323 | MS.gene55694 | 0.860103 | 2.61E-63 | -1.69E-46 |
| MS.gene058419 | MS.gene55694 | 0.877012 | 8.84E-69 | -1.69E-46 |
| MS.gene058544 | MS.gene55694 | 0.826086 | 3.29E-54 | -1.69E-46 |
| MS.gene058723 | MS.gene55694 | 0.861387 | 1.06E-63 | -1.69E-46 |
| MS.gene058773 | MS.gene55694 | 0.886721 | 2.67E-72 | -1.69E-46 |
| MS.gene059022 | MS.gene55694 | 0.880122 | 7.12E-70 | -1.69E-46 |
| MS.gene059023 | MS.gene55694 | 0.834412 | 3.04E-56 | -1.69E-46 |
| MS.gene059029 | MS.gene55694 | 0.865494 | 5.67E-65 | -1.69E-46 |
| MS.gene059079 | MS.gene55694 | 0.866262 | 3.24E-65 | -1.69E-46 |
| MS.gene059102 | MS.gene55694 | -0.823353 | 1.45E-53 | -1.69E-46 |
| MS.gene05913 | MS.gene55694 | 0.801546 | 8.56E-49 | -1.69E-46 |
| MS.gene05934 | MS.gene55694 | 0.812789 | 3.55E-51 | -1.69E-46 |
| MS.gene059384 | MS.gene55694 | 0.801711 | 7.92E-49 | -1.69E-46 |
| MS.gene059536 | MS.gene55694 | 0.812254 | 4.65E-51 | -1.69E-46 |
| MS.gene059677 | MS.gene55694 | 0.900084 | 1.04E-77 | -1.69E-46 |
| MS.gene059718 | MS.gene55694 | 0.857394 | 1.68E-62 | -1.69E-46 |
| MS.gene059756 | MS.gene55694 | 0.82667 | 2.39E-54 | -1.69E-46 |
| MS.gene059801 | MS.gene55694 | 0.800746 | 1.25E-48 | -1.69E-46 |
| MS.gene059865 | MS.gene55694 | 0.840871 | 6.70E-58 | -1.69E-46 |
| MS.gene059902 | MS.gene55694 | 0.834781 | 2.46E-56 | -1.69E-46 |
| MS.gene059960 | MS.gene55694 | 0.86944 | 3.09E-66 | -1.69E-46 |
| MS.gene060193 | MS.gene55694 | 0.845327 | 4.35E-59 | -1.69E-46 |
| MS.gene060336 | MS.gene55694 | -0.809345 | 1.98E-50 | -1.69E-46 |
| MS.gene060337 | MS.gene55694 | -0.850996 | 1.18E-60 | -1.69E-46 |
| MS.gene060703 | MS.gene55694 | 0.841073 | 5.93E-58 | -1.69E-46 |
| MS.gene060762 | MS.gene55694 | 0.863712 | 2.05E-64 | -1.69E-46 |
| MS.gene06077 | MS.gene55694 | 0.834055 | 3.74E-56 | -1.69E-46 |
| MS.gene060818 | MS.gene55694 | 0.811227 | 7.78E-51 | -1.69E-46 |
| MS.gene06092 | MS.gene55694 | 0.824267 | 8.86E-54 | -1.69E-46 |
| MS.gene060982 | MS.gene55694 | 0.821764 | 3.39E-53 | -1.69E-46 |
| MS.gene061134 | MS.gene55694 | 0.819901 | 9.10E-53 | -1.69E-46 |
| MS.gene061150 | MS.gene55694 | 0.888173 | 7.47E-73 | -1.69E-46 |
| MS.gene061171 | MS.gene55694 | 0.814972 | 1.17E-51 | -1.69E-46 |
| MS.gene061200 | MS.gene55694 | 0.877619 | 5.44E-69 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene55694.t1 | MTR_3g062930 | 94.792 | 192 | 6 | 2 | 1 | 188 | 23 | 214 | 1.10e-127 | 358 |
| MS.gene55694.t1 | MTR_3g095900 | 44.144 | 111 | 62 | 0 | 78 | 188 | 3 | 113 | 5.62e-29 | 105 |
| MS.gene55694.t1 | MTR_8g103900 | 44.144 | 111 | 62 | 0 | 78 | 188 | 3 | 113 | 8.65e-29 | 105 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene55694.t1 | AT3G54210 | 73.575 | 193 | 43 | 3 | 1 | 188 | 22 | 211 | 3.05e-92 | 268 |
| MS.gene55694.t1 | AT5G09770 | 45.946 | 111 | 60 | 0 | 78 | 188 | 3 | 113 | 1.26e-29 | 107 |
| MS.gene55694.t1 | AT5G64650 | 45.045 | 111 | 61 | 0 | 78 | 188 | 3 | 113 | 4.80e-29 | 105 |
Find 65 sgRNAs with CRISPR-Local
Find 122 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCTTGATGCGGCCATATTTA+AGG | 0.180492 | 3.3:+56983113 | None:intergenic |
| GGAAGTAGGGATTGGGATTT+GGG | 0.213556 | 3.3:+56984279 | None:intergenic |
| GGGAAGTAGGGATTGGGATT+TGG | 0.214226 | 3.3:+56984278 | None:intergenic |
| TTTCTTTCGCCGTAACGTTC+TGG | 0.272629 | 3.3:+56982947 | None:intergenic |
| AAGATGGTTCTCTACATAAA+AGG | 0.312212 | 3.3:-56983030 | MS.gene55694:CDS |
| GGGATTTGGGTCTGGAAATT+CGG | 0.343491 | 3.3:+56984292 | None:intergenic |
| ACAACACAGCTCCTTAAATA+TGG | 0.358835 | 3.3:-56983124 | MS.gene55694:CDS |
| ATTTAAGGAGCTGTGTTGTT+AGG | 0.359978 | 3.3:+56983128 | None:intergenic |
| GGGCAAGAGCCAGTGCAATT+AGG | 0.362008 | 3.3:-56983084 | MS.gene55694:CDS |
| ATGAGTGCCTTACGCTGATC+AGG | 0.382221 | 3.3:+56983157 | None:intergenic |
| CATTATCACCCCGCCTTGGT+AGG | 0.385376 | 3.3:+56982897 | None:intergenic |
| TAGGGATTGGGATTTGGGTC+TGG | 0.393604 | 3.3:+56984284 | None:intergenic |
| CCTGATGGAGGAGCGAAGAT+TGG | 0.401689 | 3.3:+56984231 | None:intergenic |
| AATTGCACTGGCTCTTGCCC+TGG | 0.404316 | 3.3:+56983087 | None:intergenic |
| GAACGTTACGGCGAAAGAAA+TGG | 0.422924 | 3.3:-56982944 | MS.gene55694:CDS |
| CCAATCTTCGCTCCTCCATC+AGG | 0.435435 | 3.3:-56984231 | MS.gene55694:intron |
| CCGACCACCATTGTCACTAA+TGG | 0.452049 | 3.3:+56983228 | None:intergenic |
| GCGGAAGTAGGAGGAATTGA+AGG | 0.464667 | 3.3:+56984351 | None:intergenic |
| GGAAAGTGCCAAAACTCAAC+AGG | 0.464799 | 3.3:-56983183 | MS.gene55694:CDS |
| TATAAGAACCCTACCAAGGC+GGG | 0.471140 | 3.3:-56982906 | MS.gene55694:CDS |
| CTGATGGAGGAGCGAAGATT+GGG | 0.477070 | 3.3:+56984232 | None:intergenic |
| TCCCTACTTCCCTCTTTCAC+CGG | 0.487319 | 3.3:-56984267 | MS.gene55694:CDS |
| GAAAGAGGGAAGTAGGGATT+GGG | 0.487973 | 3.3:+56984272 | None:intergenic |
| GATTGGGAGAGGACGAAGTC+CGG | 0.490357 | 3.3:+56984248 | None:intergenic |
| ATGATCACTCTTGCAAAAGA+TGG | 0.491383 | 3.3:-56983046 | MS.gene55694:CDS |
| AGTAGGAGGAATTGAAGGTA+AGG | 0.505266 | 3.3:+56984356 | None:intergenic |
| TGAAAGAGGGAAGTAGGGAT+TGG | 0.507962 | 3.3:+56984271 | None:intergenic |
| GCGGGGTGATAATGCACCTA+TGG | 0.509687 | 3.3:-56982888 | MS.gene55694:CDS |
| AGGACGAAGTCCGGTGAAAG+AGG | 0.511134 | 3.3:+56984257 | None:intergenic |
| CATTAGTGACAATGGTGGTC+GGG | 0.513162 | 3.3:-56983227 | MS.gene55694:CDS |
| GCAGAGGTTCCAGAACGTTA+CGG | 0.513766 | 3.3:-56982956 | MS.gene55694:CDS |
| GTCCGGTGAAAGAGGGAAGT+AGG | 0.521063 | 3.3:+56984265 | None:intergenic |
| CTGGAAATTCGGAGTGTGGT+TGG | 0.530465 | 3.3:+56984303 | None:intergenic |
| CGGGTCTATGCTATGAGACA+TGG | 0.537503 | 3.3:-56983208 | MS.gene55694:CDS |
| CAGCGTAAGGCACTCATTCG+AGG | 0.548108 | 3.3:-56983151 | MS.gene55694:CDS |
| ATTATCACCCCGCCTTGGTA+GGG | 0.549919 | 3.3:+56982898 | None:intergenic |
| TGAGTGCCTTACGCTGATCA+GGG | 0.550575 | 3.3:+56983158 | None:intergenic |
| AACATACTTCCTAATTGCAC+TGG | 0.550604 | 3.3:+56983075 | None:intergenic |
| CCATTAGTGACAATGGTGGT+CGG | 0.552255 | 3.3:-56983228 | MS.gene55694:CDS |
| TCCGGTGAAAGAGGGAAGTA+GGG | 0.554379 | 3.3:+56984266 | None:intergenic |
| CGCAATGAAGAAGCGGAAGT+AGG | 0.554403 | 3.3:+56984339 | None:intergenic |
| TGTTCATGCTTTATTTGCAG+AGG | 0.556642 | 3.3:-56982972 | MS.gene55694:CDS |
| GGACGAAGTCCGGTGAAAGA+GGG | 0.581835 | 3.3:+56984258 | None:intergenic |
| GGAGGAGCGAAGATTGGGAG+AGG | 0.593002 | 3.3:+56984237 | None:intergenic |
| ACAAGTTCAATGTATGCCAT+AGG | 0.593731 | 3.3:+56982872 | None:intergenic |
| GGGTCTGGAAATTCGGAGTG+TGG | 0.595280 | 3.3:+56984299 | None:intergenic |
| GCTTCTCATAGATGAAGCCA+AGG | 0.606561 | 3.3:+56982999 | None:intergenic |
| GGTGCATTATCACCCCGCCT+TGG | 0.608519 | 3.3:+56982893 | None:intergenic |
| AGTTTCACCATTAGTGACAA+TGG | 0.609075 | 3.3:-56983235 | MS.gene55694:CDS |
| TTGCCCTGGTTGTCTTGATG+CGG | 0.610798 | 3.3:+56983101 | None:intergenic |
| ATGGCCGCATCAAGACAACC+AGG | 0.621297 | 3.3:-56983105 | MS.gene55694:CDS |
| AATGAAGAAGCGGAAGTAGG+AGG | 0.624583 | 3.3:+56984342 | None:intergenic |
| CATAAAAGGAGACAAGCCCT+TGG | 0.625171 | 3.3:-56983016 | MS.gene55694:CDS |
| AGTGAAGCGCAATGAAGAAG+CGG | 0.636897 | 3.3:+56984332 | None:intergenic |
| GAGTGCCTTACGCTGATCAG+GGG | 0.649273 | 3.3:+56983159 | None:intergenic |
| AATGAAGGTATACCTGATGG+AGG | 0.654673 | 3.3:+56984219 | None:intergenic |
| CGTTACGGCGAAAGAAATGG+TGG | 0.664215 | 3.3:-56982941 | MS.gene55694:CDS |
| AGTGCCTTACGCTGATCAGG+GGG | 0.665172 | 3.3:+56983160 | None:intergenic |
| CTTCTCATAGATGAAGCCAA+GGG | 0.669074 | 3.3:+56983000 | None:intergenic |
| ATAAGAACCCTACCAAGGCG+GGG | 0.673186 | 3.3:-56982905 | MS.gene55694:CDS |
| TTCACCATTAGTGACAATGG+TGG | 0.695911 | 3.3:-56983232 | MS.gene55694:CDS |
| GAATTATAAGAACCCTACCA+AGG | 0.696668 | 3.3:-56982910 | MS.gene55694:CDS |
| TGGCCGCATCAAGACAACCA+GGG | 0.726099 | 3.3:-56983104 | MS.gene55694:CDS |
| TTATAAGAACCCTACCAAGG+CGG | 0.735151 | 3.3:-56982907 | MS.gene55694:CDS |
| TCTATGCTATGAGACATGGA+AGG | 0.735339 | 3.3:-56983204 | MS.gene55694:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | CCAAAAAAAAAAAAAAAACA+TGG | - | chr3.3:56983594-56983613 | MS.gene55694:intron | 15.0% |
| !!! | AAATTATGCATTTGTTTTAT+GGG | - | chr3.3:56983085-56983104 | MS.gene55694:CDS | 15.0% |
| !!! | CCATGTTTTTTTTTTTTTTT+TGG | + | chr3.3:56983597-56983616 | None:intergenic | 15.0% |
| !! | ATTTAATTATTCTATTGCCT+CGG | - | chr3.3:56983229-56983248 | MS.gene55694:CDS | 20.0% |
| !! | TAGATAATAATTACGTTTAC+AGG | - | chr3.3:56983284-56983303 | MS.gene55694:intron | 20.0% |
| !!! | AATTATGCATTTGTTTTATG+GGG | - | chr3.3:56983086-56983105 | MS.gene55694:CDS | 20.0% |
| !!! | GAAATTATGCATTTGTTTTA+TGG | - | chr3.3:56983084-56983103 | MS.gene55694:CDS | 20.0% |
| ! | AGTATAACTGGATTGAATTT+CGG | + | chr3.3:56983682-56983701 | None:intergenic | 25.0% |
| ! | AGTTATTGAAGTAGTATAAC+TGG | + | chr3.3:56983694-56983713 | None:intergenic | 25.0% |
| ! | ATAGTTCTCACCTTAAAATT+CGG | - | chr3.3:56983453-56983472 | MS.gene55694:intron | 25.0% |
| ! | CAAGATGATTCTTAAAATTC+GGG | + | chr3.3:56983504-56983523 | None:intergenic | 25.0% |
| ! | CCAAGATGATTCTTAAAATT+CGG | + | chr3.3:56983505-56983524 | None:intergenic | 25.0% |
| !! | TTTTGTATCAGCTAGAAATA+CGG | - | chr3.3:56983405-56983424 | MS.gene55694:intron | 25.0% |
| !!! | ATTTTAAGGTGAGAACTATT+CGG | + | chr3.3:56983452-56983471 | None:intergenic | 25.0% |
| AACTTGAACCAAGTAAAATG+AGG | + | chr3.3:56983837-56983856 | None:intergenic | 30.0% | |
| AAGATGGTTCTCTACATAAA+AGG | - | chr3.3:56984213-56984232 | MS.gene55694:intron | 30.0% | |
| AAGTAAGGCATAGTGTTTAA+GGG | + | chr3.3:56983635-56983654 | None:intergenic | 30.0% | |
| ATGCAATGGAAAAGAAATGA+AGG | + | chr3.3:56983042-56983061 | None:intergenic | 30.0% | |
| TGCTGAATTTCAAGAGATAA+TGG | + | chr3.3:56983941-56983960 | None:intergenic | 30.0% | |
| TTATAAACCAGAAATCCTAG+AGG | + | chr3.3:56983558-56983577 | None:intergenic | 30.0% | |
| ! | CATCAATACCTCATTTTACT+TGG | - | chr3.3:56983826-56983845 | MS.gene55694:intron | 30.0% |
| ! | TCCAACAAAACCGAATTTTA+AGG | + | chr3.3:56983466-56983485 | None:intergenic | 30.0% |
| !! | ACCTTAAAATTCGGTTTTGT+TGG | - | chr3.3:56983462-56983481 | MS.gene55694:intron | 30.0% |
| !! | AGGATTTCTGGTTTATAACA+TGG | - | chr3.3:56983560-56983579 | MS.gene55694:intron | 30.0% |
| !! | CAAGAGATAATGGATACTAT+GGG | + | chr3.3:56983931-56983950 | None:intergenic | 30.0% |
| !! | CAAGTAAAATGAGGTATTGA+TGG | + | chr3.3:56983828-56983847 | None:intergenic | 30.0% |
| !! | CCGAATTTTAAGAATCATCT+TGG | - | chr3.3:56983502-56983521 | MS.gene55694:intron | 30.0% |
| !! | TCAAGAGATAATGGATACTA+TGG | + | chr3.3:56983932-56983951 | None:intergenic | 30.0% |
| !! | TTTACTAGTAGCTTAGCTTT+TGG | - | chr3.3:56983165-56983184 | MS.gene55694:CDS | 30.0% |
| !!! | CATTTGTTTTATGGGGAAAT+TGG | - | chr3.3:56983093-56983112 | MS.gene55694:CDS | 30.0% |
| !!! | TTTTAAGGTGAGAACTATTC+GGG | + | chr3.3:56983451-56983470 | None:intergenic | 30.0% |
| AAAGAGAGTCTCTTATGCAA+TGG | + | chr3.3:56983056-56983075 | None:intergenic | 35.0% | |
| AACATACTTCCTAATTGCAC+TGG | + | chr3.3:56984171-56984190 | None:intergenic | 35.0% | |
| ACAACACAGCTCCTTAAATA+TGG | - | chr3.3:56984119-56984138 | MS.gene55694:intron | 35.0% | |
| ACAAGTTCAATGTATGCCAT+AGG | + | chr3.3:56984374-56984393 | None:intergenic | 35.0% | |
| AGTTTCACCATTAGTGACAA+TGG | - | chr3.3:56984008-56984027 | MS.gene55694:intron | 35.0% | |
| ATAACTGGATTGAATTTCGG+TGG | + | chr3.3:56983679-56983698 | None:intergenic | 35.0% | |
| ATCAGCTAGAAATACGGTAT+AGG | - | chr3.3:56983411-56983430 | MS.gene55694:intron | 35.0% | |
| ATCTATAGCGAGGACAAATT+CGG | + | chr3.3:56983358-56983377 | None:intergenic | 35.0% | |
| ATGATCACTCTTGCAAAAGA+TGG | - | chr3.3:56984197-56984216 | MS.gene55694:intron | 35.0% | |
| GAAGTAAGGCATAGTGTTTA+AGG | + | chr3.3:56983636-56983655 | None:intergenic | 35.0% | |
| GAATTATAAGAACCCTACCA+AGG | - | chr3.3:56984333-56984352 | MS.gene55694:CDS | 35.0% | |
| TTGACACAAGTAATAATCCG+AGG | + | chr3.3:56983249-56983268 | None:intergenic | 35.0% | |
| ! | AGAAATGAAGGTATACCTGA+TGG | + | chr3.3:56983030-56983049 | None:intergenic | 35.0% |
| ! | ATTTAAGGAGCTGTGTTGTT+AGG | + | chr3.3:56984118-56984137 | None:intergenic | 35.0% |
| !! | CAGATCAAAGGTGTTATCTT+AGG | - | chr3.3:56983773-56983792 | MS.gene55694:intron | 35.0% |
| !! | TGTTCATGCTTTATTTGCAG+AGG | - | chr3.3:56984271-56984290 | MS.gene55694:CDS | 35.0% |
| AGAACTTTCTGCTACTGGAA+TGG | - | chr3.3:56983750-56983769 | MS.gene55694:intron | 40.0% | |
| AGTAGGAGGAATTGAAGGTA+AGG | + | chr3.3:56982890-56982909 | None:intergenic | 40.0% | |
| ATCTATGTCACTGACGCATA+TGG | - | chr3.3:56983202-56983221 | MS.gene55694:CDS | 40.0% | |
| CTTCTCATAGATGAAGCCAA+GGG | + | chr3.3:56984246-56984265 | None:intergenic | 40.0% | |
| TCTATAGCGAGGACAAATTC+GGG | + | chr3.3:56983357-56983376 | None:intergenic | 40.0% | |
| TCTATGCTATGAGACATGGA+AGG | - | chr3.3:56984039-56984058 | MS.gene55694:intron | 40.0% | |
| TCTTGATGCGGCCATATTTA+AGG | + | chr3.3:56984133-56984152 | None:intergenic | 40.0% | |
| TGAGAAGAACTTTCTGCTAC+TGG | - | chr3.3:56983745-56983764 | MS.gene55694:intron | 40.0% | |
| TTATAAGAACCCTACCAAGG+CGG | - | chr3.3:56984336-56984355 | MS.gene55694:CDS | 40.0% | |
| TTCACCATTAGTGACAATGG+TGG | - | chr3.3:56984011-56984030 | MS.gene55694:intron | 40.0% | |
| ! | AATGAAGGTATACCTGATGG+AGG | + | chr3.3:56983027-56983046 | None:intergenic | 40.0% |
| ! | AGTGATTGGTTAGGGAAGTA+AGG | + | chr3.3:56983650-56983669 | None:intergenic | 40.0% |
| AAACACATCCACTCATGCTC+AGG | + | chr3.3:56983388-56983407 | None:intergenic | 45.0% | |
| AATGAAGAAGCGGAAGTAGG+AGG | + | chr3.3:56982904-56982923 | None:intergenic | 45.0% | |
| AGGACTGAACATCTATAGCG+AGG | + | chr3.3:56983368-56983387 | None:intergenic | 45.0% | |
| AGTGAAGCGCAATGAAGAAG+CGG | + | chr3.3:56982914-56982933 | None:intergenic | 45.0% | |
| CATAAAAGGAGACAAGCCCT+TGG | - | chr3.3:56984227-56984246 | MS.gene55694:intron | 45.0% | |
| CATTAGTGACAATGGTGGTC+GGG | - | chr3.3:56984016-56984035 | MS.gene55694:intron | 45.0% | |
| CCATTAGTGACAATGGTGGT+CGG | - | chr3.3:56984015-56984034 | MS.gene55694:intron | 45.0% | |
| CTACTGGAATGGCAGATCAA+AGG | - | chr3.3:56983761-56983780 | MS.gene55694:intron | 45.0% | |
| GAAAGAGGGAAGTAGGGATT+GGG | + | chr3.3:56982974-56982993 | None:intergenic | 45.0% | |
| GAACGTTACGGCGAAAGAAA+TGG | - | chr3.3:56984299-56984318 | MS.gene55694:CDS | 45.0% | |
| GAATCATCTTGGCACTCTTC+TGG | - | chr3.3:56983513-56983532 | MS.gene55694:intron | 45.0% | |
| GCTTCTCATAGATGAAGCCA+AGG | + | chr3.3:56984247-56984266 | None:intergenic | 45.0% | |
| GGAAGTAGGGATTGGGATTT+GGG | + | chr3.3:56982967-56982986 | None:intergenic | 45.0% | |
| GGGATTTGGGTCTGGAAATT+CGG | + | chr3.3:56982954-56982973 | None:intergenic | 45.0% | |
| TATAAGAACCCTACCAAGGC+GGG | - | chr3.3:56984337-56984356 | MS.gene55694:CDS | 45.0% | |
| TGAAAGAGGGAAGTAGGGAT+TGG | + | chr3.3:56982975-56982994 | None:intergenic | 45.0% | |
| TTTCTTTCGCCGTAACGTTC+TGG | + | chr3.3:56984299-56984318 | None:intergenic | 45.0% | |
| ! | GGACGAGAAGTGATTGGTTA+GGG | + | chr3.3:56983658-56983677 | None:intergenic | 45.0% |
| ! | TCTCATTTTCCTGCACCTCT+AGG | - | chr3.3:56983540-56983559 | MS.gene55694:intron | 45.0% |
| ! | TGGACGAGAAGTGATTGGTT+AGG | + | chr3.3:56983659-56983678 | None:intergenic | 45.0% |
| !! | GGAAAGTGCCAAAACTCAAC+AGG | - | chr3.3:56984060-56984079 | MS.gene55694:intron | 45.0% |
| ATAAGAACCCTACCAAGGCG+GGG | - | chr3.3:56984338-56984357 | MS.gene55694:CDS | 50.0% | |
| ATAGCGAGGACAAATTCGGG+TGG | + | chr3.3:56983354-56983373 | None:intergenic | 50.0% | |
| ATGAGTGCCTTACGCTGATC+AGG | + | chr3.3:56984089-56984108 | None:intergenic | 50.0% | |
| ATTATCACCCCGCCTTGGTA+GGG | + | chr3.3:56984348-56984367 | None:intergenic | 50.0% | |
| CCGACCACCATTGTCACTAA+TGG | + | chr3.3:56984018-56984037 | None:intergenic | 50.0% | |
| CGCAATGAAGAAGCGGAAGT+AGG | + | chr3.3:56982907-56982926 | None:intergenic | 50.0% | |
| CGGGTCTATGCTATGAGACA+TGG | - | chr3.3:56984035-56984054 | MS.gene55694:intron | 50.0% | |
| CGTTACGGCGAAAGAAATGG+TGG | - | chr3.3:56984302-56984321 | MS.gene55694:CDS | 50.0% | |
| CTGATGGAGGAGCGAAGATT+GGG | + | chr3.3:56983014-56983033 | None:intergenic | 50.0% | |
| CTGGAAATTCGGAGTGTGGT+TGG | + | chr3.3:56982943-56982962 | None:intergenic | 50.0% | |
| GCAGAGGTTCCAGAACGTTA+CGG | - | chr3.3:56984287-56984306 | MS.gene55694:CDS | 50.0% | |
| GCGGAAGTAGGAGGAATTGA+AGG | + | chr3.3:56982895-56982914 | None:intergenic | 50.0% | |
| GGGAAGTAGGGATTGGGATT+TGG | + | chr3.3:56982968-56982987 | None:intergenic | 50.0% | |
| TAGGGATTGGGATTTGGGTC+TGG | + | chr3.3:56982962-56982981 | None:intergenic | 50.0% | |
| TCCCTACTTCCCTCTTTCAC+CGG | - | chr3.3:56982976-56982995 | MS.gene55694:CDS | 50.0% | |
| TCCGGTGAAAGAGGGAAGTA+GGG | + | chr3.3:56982980-56982999 | None:intergenic | 50.0% | |
| TGAGTGCCTTACGCTGATCA+GGG | + | chr3.3:56984088-56984107 | None:intergenic | 50.0% | |
| TGTTCAGTCCTGAGCATGAG+TGG | - | chr3.3:56983377-56983396 | MS.gene55694:intron | 50.0% | |
| TTCGGTGGACGAGAAGTGAT+TGG | + | chr3.3:56983664-56983683 | None:intergenic | 50.0% | |
| TTGCCCTGGTTGTCTTGATG+CGG | + | chr3.3:56984145-56984164 | None:intergenic | 50.0% | |
| ! | ACCAGAAATCCTAGAGGTGC+AGG | + | chr3.3:56983552-56983571 | None:intergenic | 50.0% |
| !! | TCCTGCACCTCTAGGATTTC+TGG | - | chr3.3:56983548-56983567 | MS.gene55694:intron | 50.0% |
| AGGACGAAGTCCGGTGAAAG+AGG | + | chr3.3:56982989-56983008 | None:intergenic | 55.0% | |
| AGTGCCTTACGCTGATCAGG+GGG | + | chr3.3:56984086-56984105 | None:intergenic | 55.0% | |
| ATGGCCGCATCAAGACAACC+AGG | - | chr3.3:56984138-56984157 | MS.gene55694:intron | 55.0% | |
| CATTATCACCCCGCCTTGGT+AGG | + | chr3.3:56984349-56984368 | None:intergenic | 55.0% | |
| CCAATCTTCGCTCCTCCATC+AGG | - | chr3.3:56983012-56983031 | MS.gene55694:CDS | 55.0% | |
| CCTGATGGAGGAGCGAAGAT+TGG | + | chr3.3:56983015-56983034 | None:intergenic | 55.0% | |
| GAGTGCCTTACGCTGATCAG+GGG | + | chr3.3:56984087-56984106 | None:intergenic | 55.0% | |
| GATTGGGAGAGGACGAAGTC+CGG | + | chr3.3:56982998-56983017 | None:intergenic | 55.0% | |
| GGACGAAGTCCGGTGAAAGA+GGG | + | chr3.3:56982988-56983007 | None:intergenic | 55.0% | |
| GGGCAAGAGCCAGTGCAATT+AGG | - | chr3.3:56984159-56984178 | MS.gene55694:intron | 55.0% | |
| GGGTCTGGAAATTCGGAGTG+TGG | + | chr3.3:56982947-56982966 | None:intergenic | 55.0% | |
| GTCCGGTGAAAGAGGGAAGT+AGG | + | chr3.3:56982981-56983000 | None:intergenic | 55.0% | |
| TGGCCGCATCAAGACAACCA+GGG | - | chr3.3:56984139-56984158 | MS.gene55694:intron | 55.0% | |
| ! | CAGCGTAAGGCACTCATTCG+AGG | - | chr3.3:56984092-56984111 | MS.gene55694:intron | 55.0% |
| ! | GCGGGGTGATAATGCACCTA+TGG | - | chr3.3:56984355-56984374 | MS.gene55694:CDS | 55.0% |
| ! | TCAGGGGGCCTGTTGAGTTT+TGG | + | chr3.3:56984071-56984090 | None:intergenic | 55.0% |
| !! | AATTGCACTGGCTCTTGCCC+TGG | + | chr3.3:56984159-56984178 | None:intergenic | 55.0% |
| GGAGGAGCGAAGATTGGGAG+AGG | + | chr3.3:56983009-56983028 | None:intergenic | 60.0% | |
| GGTGCATTATCACCCCGCCT+TGG | + | chr3.3:56984353-56984372 | None:intergenic | 60.0% | |
| CAGGCCCCCTGATCAGCGTA+AGG | - | chr3.3:56984079-56984098 | MS.gene55694:intron | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 56982868 | 56984397 | 56982868 | ID=MS.gene55694 |
| chr3.3 | mRNA | 56982868 | 56984397 | 56982868 | ID=MS.gene55694.t1;Parent=MS.gene55694 |
| chr3.3 | exon | 56984232 | 56984397 | 56984232 | ID=MS.gene55694.t1.exon1;Parent=MS.gene55694.t1 |
| chr3.3 | CDS | 56984232 | 56984397 | 56984232 | ID=cds.MS.gene55694.t1;Parent=MS.gene55694.t1 |
| chr3.3 | exon | 56982868 | 56983268 | 56982868 | ID=MS.gene55694.t1.exon2;Parent=MS.gene55694.t1 |
| chr3.3 | CDS | 56982868 | 56983268 | 56982868 | ID=cds.MS.gene55694.t1;Parent=MS.gene55694.t1 |
| Gene Sequence |
| Protein sequence |
