AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene66460


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene66460.t1 MTR_1g018030 95.361 194 7 2 2 195 1 192 2.91e-135 376
MS.gene66460.t1 MTR_7g073990 42.953 149 74 2 47 187 97 242 1.69e-31 114
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene66460.t1 AT1G26220 61.386 202 65 5 2 195 1 197 2.53e-79 235
MS.gene66460.t1 AT1G32070 41.985 131 60 1 76 190 127 257 6.73e-29 108
MS.gene66460.t1 AT1G32070 40.909 132 61 1 76 190 126 257 6.24e-28 105
MS.gene66460.t1 AT1G32070 40.909 132 61 1 76 190 127 258 6.37e-28 105

Find 50 sgRNAs with CRISPR-Local

Find 60 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATACTTTGTTAAGTGCTTCT+AGG 0.216082 1.4:+4948751 None:intergenic
TACTCTGAGCCAAGAGTTCT+TGG 0.233326 1.4:-4948465 MS.gene66460:CDS
AACAACAACATCCCATATTA+TGG 0.310068 1.4:+4948581 None:intergenic
GTTGTTGATCCTTCGTTTCA+AGG 0.353170 1.4:-4948564 MS.gene66460:CDS
CTCAGAGTAAAGAGCAATAT+TGG 0.361910 1.4:+4948479 None:intergenic
GATCCTTCGTTTCAAGGGCT+TGG 0.366151 1.4:-4948558 MS.gene66460:CDS
TCGTTTCAAGGGCTTGGACT+AGG 0.403696 1.4:-4948552 MS.gene66460:CDS
CTGTTGTCGAGAGGCTTATG+AGG 0.408843 1.4:-4948524 MS.gene66460:CDS
AAGTTAAGTCGTAGGGAGGA+TGG 0.419412 1.4:+4948909 None:intergenic
CGTTTCAAGGGCTTGGACTA+GGG 0.426846 1.4:-4948551 MS.gene66460:CDS
CGATAAAAGCCAAGAACTCT+TGG 0.431331 1.4:+4948456 None:intergenic
TAGGCTTGCTTGACAAGAAA+TGG 0.442809 1.4:+4948851 None:intergenic
TTGTTGATCCTTCGTTTCAA+GGG 0.483590 1.4:-4948563 MS.gene66460:CDS
GTTGCATTTGCAAGAGCTAC+TGG 0.489343 1.4:-4948621 MS.gene66460:CDS
ATTCTCCGCCGAACCGCAGA+GGG 0.489832 1.4:-4948780 MS.gene66460:CDS
GGTTTAACCCCTCTGCGGTT+CGG 0.494949 1.4:+4948772 None:intergenic
TTTGAAGAAGTTAAGTCGTA+GGG 0.496530 1.4:+4948902 None:intergenic
TGAGGGATCTTGTAGGAAGA+GGG 0.520334 1.4:-4948506 MS.gene66460:CDS
ACTCGAGCACACTGACTCAA+TGG 0.522170 1.4:-4948679 MS.gene66460:CDS
ATTTGAAGAAGTTAAGTCGT+AGG 0.522993 1.4:+4948901 None:intergenic
ATGAGGGATCTTGTAGGAAG+AGG 0.534453 1.4:-4948507 MS.gene66460:CDS
AGTCGTAGGGAGGATGGAAG+AGG 0.540218 1.4:+4948915 None:intergenic
ATATTGAGAGTGGGTAGTCT+AGG 0.541441 1.4:+4948832 None:intergenic
ACCATTCCACGAATACCATC+AGG 0.547694 1.4:+4948411 None:intergenic
AGGCTTATGAGGGATCTTGT+AGG 0.549706 1.4:-4948513 MS.gene66460:CDS
AGCACACTGACTCAATGGTA+TGG 0.558828 1.4:-4948674 MS.gene66460:CDS
ACAAAGTATTCGTAGCAGTA+GGG 0.561588 1.4:-4948737 MS.gene66460:CDS
TCCTGATGGTATTCGTGGAA+TGG 0.563938 1.4:-4948412 MS.gene66460:CDS
AAATGGTGAGGTTTCGTAGT+GGG 0.566008 1.4:+4948868 None:intergenic
AACAAAGTATTCGTAGCAGT+AGG 0.566118 1.4:-4948738 MS.gene66460:CDS
TTAACCCCTCTGCGGTTCGG+CGG 0.568840 1.4:+4948775 None:intergenic
GAAGAAGTTAAGTCGTAGGG+AGG 0.570827 1.4:+4948905 None:intergenic
GCTGATCCTGATGGTATTCG+TGG 0.578470 1.4:-4948417 MS.gene66460:CDS
CATTCTCCGCCGAACCGCAG+AGG 0.581744 1.4:-4948781 MS.gene66460:CDS
GTTTCGTCGGATATTGAGAG+TGG 0.593105 1.4:+4948822 None:intergenic
TTGCTTGACAAGAAATGGTG+AGG 0.596575 1.4:+4948856 None:intergenic
GATAATATGCTGCTCTTACG+TGG 0.618724 1.4:-4948951 None:intergenic
AGTCCAAGCCCTTGAAACGA+AGG 0.624386 1.4:+4948555 None:intergenic
TGTTGTCGAGAGGCTTATGA+GGG 0.625783 1.4:-4948523 MS.gene66460:CDS
TTTGCAAGAGCTACTGGTGA+TGG 0.636078 1.4:-4948615 MS.gene66460:CDS
TTCTAGGTTTAACCCCTCTG+CGG 0.644451 1.4:+4948767 None:intergenic
TTTCGTCGGATATTGAGAGT+GGG 0.648674 1.4:+4948823 None:intergenic
TAGGGAAAGCTGTTGTCGAG+AGG 0.652617 1.4:-4948533 MS.gene66460:CDS
CAATGGTATGGATACAAGAG+AGG 0.657246 1.4:-4948662 MS.gene66460:CDS
TCGTAGCAGTAGGGTTTCCG+CGG 0.663293 1.4:-4948728 MS.gene66460:CDS
GGATCAGCAACAAAACCCAA+AGG 0.674227 1.4:+4948432 None:intergenic
GAGGAAGTCGCAGAATAATG+CGG 0.702415 1.4:-4948643 MS.gene66460:CDS
GAAATGGTGAGGTTTCGTAG+TGG 0.706232 1.4:+4948867 None:intergenic
GACGAAACCCTAGAAAAGCG+AGG 0.715831 1.4:-4948807 MS.gene66460:CDS
TTCTCCGCCGAACCGCAGAG+GGG 0.756624 1.4:-4948779 MS.gene66460:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TGTTTTCAATGCCATAATAT+GGG - chr1.4:4948733-4948752 MS.gene66460:CDS 25.0%
AACAACAACATCCCATATTA+TGG + chr1.4:4948747-4948766 None:intergenic 30.0%
ATACTTTGTTAAGTGCTTCT+AGG + chr1.4:4948577-4948596 None:intergenic 30.0%
ATTTGAAGAAGTTAAGTCGT+AGG + chr1.4:4948427-4948446 None:intergenic 30.0%
TTTGAAGAAGTTAAGTCGTA+GGG + chr1.4:4948426-4948445 None:intergenic 30.0%
! GTGTTTTCAATGCCATAATA+TGG - chr1.4:4948732-4948751 MS.gene66460:CDS 30.0%
CTCAGAGTAAAGAGCAATAT+TGG + chr1.4:4948849-4948868 None:intergenic 35.0%
TTGTTGATCCTTCGTTTCAA+GGG - chr1.4:4948762-4948781 MS.gene66460:CDS 35.0%
! AACAAAGTATTCGTAGCAGT+AGG - chr1.4:4948587-4948606 MS.gene66460:CDS 35.0%
! ACAAAGTATTCGTAGCAGTA+GGG - chr1.4:4948588-4948607 MS.gene66460:CDS 35.0%
!! AATGAAACCTCGCTTTTCTA+GGG + chr1.4:4948528-4948547 None:intergenic 35.0%
AAATGGTGAGGTTTCGTAGT+GGG + chr1.4:4948460-4948479 None:intergenic 40.0%
ATATTGAGAGTGGGTAGTCT+AGG + chr1.4:4948496-4948515 None:intergenic 40.0%
CAATGGTATGGATACAAGAG+AGG - chr1.4:4948663-4948682 MS.gene66460:CDS 40.0%
CGATAAAAGCCAAGAACTCT+TGG + chr1.4:4948872-4948891 None:intergenic 40.0%
GTTGTTGATCCTTCGTTTCA+AGG - chr1.4:4948761-4948780 MS.gene66460:CDS 40.0%
TTGCTTGACAAGAAATGGTG+AGG + chr1.4:4948472-4948491 None:intergenic 40.0%
TTTCGTCGGATATTGAGAGT+GGG + chr1.4:4948505-4948524 None:intergenic 40.0%
! AGTGCAATGCGTATTTTCTC+AGG + chr1.4:4948629-4948648 None:intergenic 40.0%
! GTGCAATGCGTATTTTCTCA+GGG + chr1.4:4948628-4948647 None:intergenic 40.0%
! TAGGCTTGCTTGACAAGAAA+TGG + chr1.4:4948477-4948496 None:intergenic 40.0%
!! CTTGGCTTTTATCGACCTTT+GGG - chr1.4:4948878-4948897 MS.gene66460:CDS 40.0%
!! GAATGAAACCTCGCTTTTCT+AGG + chr1.4:4948529-4948548 None:intergenic 40.0%
!! TCTTGGCTTTTATCGACCTT+TGG - chr1.4:4948877-4948896 MS.gene66460:CDS 40.0%
AAGTTAAGTCGTAGGGAGGA+TGG + chr1.4:4948419-4948438 None:intergenic 45.0%
ACCATTCCACGAATACCATC+AGG + chr1.4:4948917-4948936 None:intergenic 45.0%
AGGCTTATGAGGGATCTTGT+AGG - chr1.4:4948812-4948831 MS.gene66460:CDS 45.0%
ATGAGGGATCTTGTAGGAAG+AGG - chr1.4:4948818-4948837 MS.gene66460:CDS 45.0%
GAAATGGTGAGGTTTCGTAG+TGG + chr1.4:4948461-4948480 None:intergenic 45.0%
GAAGAAGTTAAGTCGTAGGG+AGG + chr1.4:4948423-4948442 None:intergenic 45.0%
GAGGAAGTCGCAGAATAATG+CGG - chr1.4:4948682-4948701 MS.gene66460:CDS 45.0%
GGATCAGCAACAAAACCCAA+AGG + chr1.4:4948896-4948915 None:intergenic 45.0%
GTTGCATTTGCAAGAGCTAC+TGG - chr1.4:4948704-4948723 MS.gene66460:CDS 45.0%
GTTTCGTCGGATATTGAGAG+TGG + chr1.4:4948506-4948525 None:intergenic 45.0%
TACTCTGAGCCAAGAGTTCT+TGG - chr1.4:4948860-4948879 MS.gene66460:CDS 45.0%
TCCTGATGGTATTCGTGGAA+TGG - chr1.4:4948913-4948932 MS.gene66460:CDS 45.0%
TGAGGGATCTTGTAGGAAGA+GGG - chr1.4:4948819-4948838 MS.gene66460:CDS 45.0%
TGTTGTCGAGAGGCTTATGA+GGG - chr1.4:4948802-4948821 MS.gene66460:CDS 45.0%
TTCTAGGTTTAACCCCTCTG+CGG + chr1.4:4948561-4948580 None:intergenic 45.0%
TTTGCAAGAGCTACTGGTGA+TGG - chr1.4:4948710-4948729 MS.gene66460:CDS 45.0%
!! AGCACACTGACTCAATGGTA+TGG - chr1.4:4948651-4948670 MS.gene66460:CDS 45.0%
!!! GGTTTTGTTGCTGATCCTGA+TGG - chr1.4:4948899-4948918 MS.gene66460:CDS 45.0%
!!! TCGCTTTTCTAGGGTTTCGT+CGG + chr1.4:4948519-4948538 None:intergenic 45.0%
AGTCCAAGCCCTTGAAACGA+AGG + chr1.4:4948773-4948792 None:intergenic 50.0%
CGTTTCAAGGGCTTGGACTA+GGG - chr1.4:4948774-4948793 MS.gene66460:CDS 50.0%
CTGTTGTCGAGAGGCTTATG+AGG - chr1.4:4948801-4948820 MS.gene66460:CDS 50.0%
GACGAAACCCTAGAAAAGCG+AGG - chr1.4:4948518-4948537 MS.gene66460:CDS 50.0%
GATCCTTCGTTTCAAGGGCT+TGG - chr1.4:4948767-4948786 MS.gene66460:CDS 50.0%
GCTGATCCTGATGGTATTCG+TGG - chr1.4:4948908-4948927 MS.gene66460:CDS 50.0%
TAGGGAAAGCTGTTGTCGAG+AGG - chr1.4:4948792-4948811 MS.gene66460:CDS 50.0%
TCGTTTCAAGGGCTTGGACT+AGG - chr1.4:4948773-4948792 MS.gene66460:CDS 50.0%
!! ACTCGAGCACACTGACTCAA+TGG - chr1.4:4948646-4948665 MS.gene66460:CDS 50.0%
AGTCGTAGGGAGGATGGAAG+AGG + chr1.4:4948413-4948432 None:intergenic 55.0%
GGTTTAACCCCTCTGCGGTT+CGG + chr1.4:4948556-4948575 None:intergenic 55.0%
TCGTAGCAGTAGGGTTTCCG+CGG - chr1.4:4948597-4948616 MS.gene66460:CDS 55.0%
! ATTTTCTCAGGGTTGCGCCG+CGG + chr1.4:4948617-4948636 None:intergenic 55.0%
ATTCTCCGCCGAACCGCAGA+GGG - chr1.4:4948545-4948564 MS.gene66460:CDS 60.0%
! TTAACCCCTCTGCGGTTCGG+CGG + chr1.4:4948553-4948572 None:intergenic 60.0%
CATTCTCCGCCGAACCGCAG+AGG - chr1.4:4948544-4948563 MS.gene66460:CDS 65.0%
TTCTCCGCCGAACCGCAGAG+GGG - chr1.4:4948546-4948565 MS.gene66460:CDS 65.0%


Chromosome Type Strat End Strand Name
chr1.4 gene 4948380 4948967 4948380 ID=MS.gene66460
chr1.4 mRNA 4948380 4948967 4948380 ID=MS.gene66460.t1;Parent=MS.gene66460
chr1.4 exon 4948380 4948967 4948380 ID=MS.gene66460.t1.exon1;Parent=MS.gene66460.t1
chr1.4 CDS 4948380 4948967 4948380 ID=cds.MS.gene66460.t1;Parent=MS.gene66460.t1
Gene Sequence

>MS.gene66460

ATGCTGCTCTTACGTGGCAACATCTATGCTCCTCTTCCATCCTCCCTACGACTTAACTTCTTCAAATCACAAACTCACCCACTACGAAACCTCACCATTTCTTGTCAAGCAAGCCTAGACTACCCACTCTCAATATCCGACGAAACCCTAGAAAAGCGAGGTTTCATTCTCCGCCGAACCGCAGAGGGGTTAAACCTAGAAGCACTTAACAAAGTATTCGTAGCAGTAGGGTTTCCGCGGCGCAACCCTGAGAAAATACGCATTGCACTCGAGCACACTGACTCAATGGTATGGATACAAGAGAGGAAGTCGCAGAATAATGCGGTTGCATTTGCAAGAGCTACTGGTGATGGTGTTTTCAATGCCATAATATGGGATGTTGTTGTTGATCCTTCGTTTCAAGGGCTTGGACTAGGGAAAGCTGTTGTCGAGAGGCTTATGAGGGATCTTGTAGGAAGAGGGATTACCAATATTGCTCTTTACTCTGAGCCAAGAGTTCTTGGCTTTTATCGACCTTTGGGTTTTGTTGCTGATCCTGATGGTATTCGTGGAATGGTTTACTCGACAAAAAACAAAAGAAAAAAATAG

Protein sequence

>MS.gene66460.t1

MLLLRGNIYAPLPSSLRLNFFKSQTHPLRNLTISCQASLDYPLSISDETLEKRGFILRRTAEGLNLEALNKVFVAVGFPRRNPEKIRIALEHTDSMVWIQERKSQNNAVAFARATGDGVFNAIIWDVVVDPSFQGLGLGKAVVERLMRDLVGRGITNIALYSEPRVLGFYRPLGFVADPDGIRGMVYSTKNKRKK