Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene85923.t1 | XP_003614000.1 | 98.3 | 176 | 3 | 0 | 1 | 176 | 1 | 176 | 2.50E-91 | 344.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene85923.t1 | B7FMG8 | 98.3 | 176 | 3 | 0 | 1 | 176 | 1 | 176 | 1.8e-91 | 344.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049171 | MS.gene85923 | 0.869959 | 2.09E-66 | -1.69E-46 |
| MS.gene049173 | MS.gene85923 | 0.867766 | 1.07E-65 | -1.69E-46 |
| MS.gene049217 | MS.gene85923 | 0.87955 | 1.14E-69 | -1.69E-46 |
| MS.gene049222 | MS.gene85923 | 0.874231 | 7.94E-68 | -1.69E-46 |
| MS.gene049285 | MS.gene85923 | 0.901912 | 1.66E-78 | -1.69E-46 |
| MS.gene049320 | MS.gene85923 | 0.876364 | 1.48E-68 | -1.69E-46 |
| MS.gene049369 | MS.gene85923 | 0.807501 | 4.90E-50 | -1.69E-46 |
| MS.gene049427 | MS.gene85923 | 0.846458 | 2.15E-59 | -1.69E-46 |
| MS.gene049609 | MS.gene85923 | 0.846107 | 2.67E-59 | -1.69E-46 |
| MS.gene049614 | MS.gene85923 | 0.833092 | 6.50E-56 | -1.69E-46 |
| MS.gene049627 | MS.gene85923 | 0.803146 | 4.01E-49 | -1.69E-46 |
| MS.gene049633 | MS.gene85923 | 0.846232 | 2.47E-59 | -1.69E-46 |
| MS.gene049805 | MS.gene85923 | 0.846115 | 2.66E-59 | -1.69E-46 |
| MS.gene05010 | MS.gene85923 | 0.805556 | 1.26E-49 | -1.69E-46 |
| MS.gene050163 | MS.gene85923 | 0.884743 | 1.48E-71 | -1.69E-46 |
| MS.gene050165 | MS.gene85923 | 0.882569 | 9.33E-71 | -1.69E-46 |
| MS.gene050323 | MS.gene85923 | 0.812469 | 4.17E-51 | -1.69E-46 |
| MS.gene050333 | MS.gene85923 | 0.886493 | 3.26E-72 | -1.69E-46 |
| MS.gene050432 | MS.gene85923 | 0.82632 | 2.89E-54 | -1.69E-46 |
| MS.gene050444 | MS.gene85923 | 0.896912 | 2.34E-76 | -1.69E-46 |
| MS.gene050504 | MS.gene85923 | 0.864535 | 1.13E-64 | -1.69E-46 |
| MS.gene050518 | MS.gene85923 | 0.855296 | 6.94E-62 | -1.69E-46 |
| MS.gene050577 | MS.gene85923 | 0.810242 | 1.27E-50 | -1.69E-46 |
| MS.gene050593 | MS.gene85923 | 0.834828 | 2.39E-56 | -1.69E-46 |
| MS.gene050612 | MS.gene85923 | 0.871783 | 5.25E-67 | -1.69E-46 |
| MS.gene050675 | MS.gene85923 | 0.852524 | 4.36E-61 | -1.69E-46 |
| MS.gene050699 | MS.gene85923 | 0.853475 | 2.33E-61 | -1.69E-46 |
| MS.gene050747 | MS.gene85923 | 0.827361 | 1.63E-54 | -1.69E-46 |
| MS.gene050767 | MS.gene85923 | 0.838941 | 2.13E-57 | -1.69E-46 |
| MS.gene050770 | MS.gene85923 | 0.844644 | 6.65E-59 | -1.69E-46 |
| MS.gene050772 | MS.gene85923 | 0.869084 | 4.04E-66 | -1.69E-46 |
| MS.gene05079 | MS.gene85923 | -0.815026 | 1.14E-51 | -1.69E-46 |
| MS.gene05080 | MS.gene85923 | 0.833497 | 5.15E-56 | -1.69E-46 |
| MS.gene050868 | MS.gene85923 | 0.835428 | 1.69E-56 | -1.69E-46 |
| MS.gene050871 | MS.gene85923 | 0.83204 | 1.19E-55 | -1.69E-46 |
| MS.gene050950 | MS.gene85923 | 0.82389 | 1.09E-53 | -1.69E-46 |
| MS.gene050986 | MS.gene85923 | 0.834998 | 2.17E-56 | -1.69E-46 |
| MS.gene05100 | MS.gene85923 | 0.856723 | 2.65E-62 | -1.69E-46 |
| MS.gene05105 | MS.gene85923 | 0.80249 | 5.47E-49 | -1.69E-46 |
| MS.gene051228 | MS.gene85923 | 0.907003 | 8.10E-81 | -1.69E-46 |
| MS.gene051317 | MS.gene85923 | 0.809292 | 2.03E-50 | -1.69E-46 |
| MS.gene051434 | MS.gene85923 | 0.810302 | 1.23E-50 | -1.69E-46 |
| MS.gene051551 | MS.gene85923 | 0.824961 | 6.08E-54 | -1.69E-46 |
| MS.gene051617 | MS.gene85923 | 0.850566 | 1.56E-60 | -1.69E-46 |
| MS.gene051666 | MS.gene85923 | 0.872843 | 2.33E-67 | -1.69E-46 |
| MS.gene051692 | MS.gene85923 | 0.823123 | 1.64E-53 | -1.69E-46 |
| MS.gene051694 | MS.gene85923 | 0.823563 | 1.29E-53 | -1.69E-46 |
| MS.gene051734 | MS.gene85923 | 0.804102 | 2.54E-49 | -1.69E-46 |
| MS.gene051810 | MS.gene85923 | 0.881934 | 1.59E-70 | -1.69E-46 |
| MS.gene051838 | MS.gene85923 | 0.809134 | 2.20E-50 | -1.69E-46 |
| MS.gene051858 | MS.gene85923 | 0.884045 | 2.68E-71 | -1.69E-46 |
| MS.gene051874 | MS.gene85923 | 0.814143 | 1.79E-51 | -1.69E-46 |
| MS.gene051949 | MS.gene85923 | 0.904109 | 1.73E-79 | -1.69E-46 |
| MS.gene052129 | MS.gene85923 | -0.806426 | 8.27E-50 | -1.69E-46 |
| MS.gene052164 | MS.gene85923 | 0.815791 | 7.71E-52 | -1.69E-46 |
| MS.gene052271 | MS.gene85923 | 0.878814 | 2.07E-69 | -1.69E-46 |
| MS.gene052290 | MS.gene85923 | 0.850344 | 1.80E-60 | -1.69E-46 |
| MS.gene052379 | MS.gene85923 | 0.902856 | 6.32E-79 | -1.69E-46 |
| MS.gene052484 | MS.gene85923 | 0.832035 | 1.19E-55 | -1.69E-46 |
| MS.gene052503 | MS.gene85923 | 0.805974 | 1.03E-49 | -1.69E-46 |
| MS.gene052615 | MS.gene85923 | 0.827688 | 1.36E-54 | -1.69E-46 |
| MS.gene052822 | MS.gene85923 | 0.813094 | 3.05E-51 | -1.69E-46 |
| MS.gene052870 | MS.gene85923 | 0.902525 | 8.87E-79 | -1.69E-46 |
| MS.gene052872 | MS.gene85923 | 0.901378 | 2.85E-78 | -1.69E-46 |
| MS.gene052916 | MS.gene85923 | 0.824311 | 8.65E-54 | -1.69E-46 |
| MS.gene052966 | MS.gene85923 | 0.821847 | 3.25E-53 | -1.69E-46 |
| MS.gene053009 | MS.gene85923 | 0.837963 | 3.81E-57 | -1.69E-46 |
| MS.gene053103 | MS.gene85923 | 0.850324 | 1.83E-60 | -1.69E-46 |
| MS.gene053104 | MS.gene85923 | 0.880247 | 6.42E-70 | -1.69E-46 |
| MS.gene053107 | MS.gene85923 | 0.810524 | 1.10E-50 | -1.69E-46 |
| MS.gene053203 | MS.gene85923 | 0.80315 | 4.00E-49 | -1.69E-46 |
| MS.gene053207 | MS.gene85923 | 0.846623 | 1.93E-59 | -1.69E-46 |
| MS.gene053213 | MS.gene85923 | 0.859697 | 3.46E-63 | -1.69E-46 |
| MS.gene053286 | MS.gene85923 | 0.855435 | 6.32E-62 | -1.69E-46 |
| MS.gene053322 | MS.gene85923 | 0.871388 | 7.10E-67 | -1.69E-46 |
| MS.gene053384 | MS.gene85923 | 0.848347 | 6.49E-60 | -1.69E-46 |
| MS.gene053449 | MS.gene85923 | 0.818645 | 1.76E-52 | -1.69E-46 |
| MS.gene053479 | MS.gene85923 | 0.814042 | 1.88E-51 | -1.69E-46 |
| MS.gene053574 | MS.gene85923 | 0.82317 | 1.60E-53 | -1.69E-46 |
| MS.gene053632 | MS.gene85923 | 0.877252 | 7.30E-69 | -1.69E-46 |
| MS.gene053824 | MS.gene85923 | 0.863861 | 1.84E-64 | -1.69E-46 |
| MS.gene053916 | MS.gene85923 | 0.849438 | 3.23E-60 | -1.69E-46 |
| MS.gene054163 | MS.gene85923 | 0.813486 | 2.50E-51 | -1.69E-46 |
| MS.gene054376 | MS.gene85923 | 0.819497 | 1.13E-52 | -1.69E-46 |
| MS.gene054408 | MS.gene85923 | 0.80868 | 2.75E-50 | -1.69E-46 |
| MS.gene054543 | MS.gene85923 | 0.819085 | 1.40E-52 | -1.69E-46 |
| MS.gene054595 | MS.gene85923 | 0.818572 | 1.83E-52 | -1.69E-46 |
| MS.gene054600 | MS.gene85923 | 0.872632 | 2.74E-67 | -1.69E-46 |
| MS.gene054642 | MS.gene85923 | 0.898335 | 5.87E-77 | -1.69E-46 |
| MS.gene05470 | MS.gene85923 | 0.81921 | 1.31E-52 | -1.69E-46 |
| MS.gene05494 | MS.gene85923 | 0.802435 | 5.62E-49 | -1.69E-46 |
| MS.gene054954 | MS.gene85923 | 0.869823 | 2.32E-66 | -1.69E-46 |
| MS.gene054955 | MS.gene85923 | 0.801539 | 8.59E-49 | -1.69E-46 |
| MS.gene05505 | MS.gene85923 | 0.841544 | 4.46E-58 | -1.69E-46 |
| MS.gene055206 | MS.gene85923 | 0.881406 | 2.46E-70 | -1.69E-46 |
| MS.gene055212 | MS.gene85923 | 0.85556 | 5.81E-62 | -1.69E-46 |
| MS.gene055224 | MS.gene85923 | 0.814498 | 1.49E-51 | -1.69E-46 |
| MS.gene055254 | MS.gene85923 | 0.830031 | 3.69E-55 | -1.69E-46 |
| MS.gene055289 | MS.gene85923 | 0.81383 | 2.10E-51 | -1.69E-46 |
| MS.gene055362 | MS.gene85923 | 0.871065 | 9.08E-67 | -1.69E-46 |
| MS.gene055397 | MS.gene85923 | 0.865932 | 4.13E-65 | -1.69E-46 |
| MS.gene055499 | MS.gene85923 | 0.842797 | 2.08E-58 | -1.69E-46 |
| MS.gene055502 | MS.gene85923 | 0.876331 | 1.52E-68 | -1.69E-46 |
| MS.gene055577 | MS.gene85923 | 0.866974 | 1.92E-65 | -1.69E-46 |
| MS.gene055636 | MS.gene85923 | 0.867404 | 1.40E-65 | -1.69E-46 |
| MS.gene055638 | MS.gene85923 | 0.861303 | 1.13E-63 | -1.69E-46 |
| MS.gene055647 | MS.gene85923 | 0.843856 | 1.08E-58 | -1.69E-46 |
| MS.gene055722 | MS.gene85923 | 0.88597 | 5.14E-72 | -1.69E-46 |
| MS.gene055748 | MS.gene85923 | 0.850155 | 2.04E-60 | -1.69E-46 |
| MS.gene055750 | MS.gene85923 | 0.832989 | 6.90E-56 | -1.69E-46 |
| MS.gene055751 | MS.gene85923 | 0.852769 | 3.71E-61 | -1.69E-46 |
| MS.gene055776 | MS.gene85923 | 0.809276 | 2.05E-50 | -1.69E-46 |
| MS.gene05589 | MS.gene85923 | 0.811446 | 6.97E-51 | -1.69E-46 |
| MS.gene05592 | MS.gene85923 | 0.875942 | 2.07E-68 | -1.69E-46 |
| MS.gene055941 | MS.gene85923 | 0.884853 | 1.34E-71 | -1.69E-46 |
| MS.gene055956 | MS.gene85923 | -0.816249 | 6.10E-52 | -1.69E-46 |
| MS.gene056083 | MS.gene85923 | 0.835515 | 1.60E-56 | -1.69E-46 |
| MS.gene056374 | MS.gene85923 | 0.871451 | 6.76E-67 | -1.69E-46 |
| MS.gene056449 | MS.gene85923 | 0.829 | 6.58E-55 | -1.69E-46 |
| MS.gene056487 | MS.gene85923 | 0.807625 | 4.61E-50 | -1.69E-46 |
| MS.gene056504 | MS.gene85923 | 0.833461 | 5.26E-56 | -1.69E-46 |
| MS.gene056510 | MS.gene85923 | 0.861458 | 1.01E-63 | -1.69E-46 |
| MS.gene056549 | MS.gene85923 | 0.857704 | 1.36E-62 | -1.69E-46 |
| MS.gene056609 | MS.gene85923 | 0.839566 | 1.47E-57 | -1.69E-46 |
| MS.gene056657 | MS.gene85923 | 0.892414 | 1.62E-74 | -1.69E-46 |
| MS.gene056692 | MS.gene85923 | 0.826644 | 2.42E-54 | -1.69E-46 |
| MS.gene056897 | MS.gene85923 | 0.866324 | 3.10E-65 | -1.69E-46 |
| MS.gene056898 | MS.gene85923 | 0.818552 | 1.85E-52 | -1.69E-46 |
| MS.gene056903 | MS.gene85923 | 0.820948 | 5.24E-53 | -1.69E-46 |
| MS.gene056946 | MS.gene85923 | 0.825892 | 3.66E-54 | -1.69E-46 |
| MS.gene056947 | MS.gene85923 | 0.816653 | 4.95E-52 | -1.69E-46 |
| MS.gene057011 | MS.gene85923 | 0.81878 | 1.64E-52 | -1.69E-46 |
| MS.gene057076 | MS.gene85923 | 0.834458 | 2.96E-56 | -1.69E-46 |
| MS.gene057129 | MS.gene85923 | 0.89004 | 1.41E-73 | -1.69E-46 |
| MS.gene057146 | MS.gene85923 | 0.823022 | 1.73E-53 | -1.69E-46 |
| MS.gene057246 | MS.gene85923 | 0.849747 | 2.65E-60 | -1.69E-46 |
| MS.gene057331 | MS.gene85923 | 0.846501 | 2.09E-59 | -1.69E-46 |
| MS.gene057416 | MS.gene85923 | 0.853136 | 2.92E-61 | -1.69E-46 |
| MS.gene057658 | MS.gene85923 | 0.830073 | 3.60E-55 | -1.69E-46 |
| MS.gene057734 | MS.gene85923 | 0.801621 | 8.26E-49 | -1.69E-46 |
| MS.gene057805 | MS.gene85923 | 0.818832 | 1.60E-52 | -1.69E-46 |
| MS.gene057831 | MS.gene85923 | 0.834774 | 2.47E-56 | -1.69E-46 |
| MS.gene057856 | MS.gene85923 | 0.813165 | 2.94E-51 | -1.69E-46 |
| MS.gene057857 | MS.gene85923 | 0.838759 | 2.38E-57 | -1.69E-46 |
| MS.gene057926 | MS.gene85923 | 0.872817 | 2.38E-67 | -1.69E-46 |
| MS.gene058006 | MS.gene85923 | 0.811357 | 7.29E-51 | -1.69E-46 |
| MS.gene058019 | MS.gene85923 | 0.829041 | 6.43E-55 | -1.69E-46 |
| MS.gene058293 | MS.gene85923 | 0.802553 | 5.31E-49 | -1.69E-46 |
| MS.gene058323 | MS.gene85923 | 0.825398 | 4.79E-54 | -1.69E-46 |
| MS.gene058338 | MS.gene85923 | 0.815255 | 1.02E-51 | -1.69E-46 |
| MS.gene05837 | MS.gene85923 | 0.806911 | 6.54E-50 | -1.69E-46 |
| MS.gene058419 | MS.gene85923 | 0.892903 | 1.03E-74 | -1.69E-46 |
| MS.gene058544 | MS.gene85923 | 0.849986 | 2.27E-60 | -1.69E-46 |
| MS.gene058588 | MS.gene85923 | 0.802778 | 4.77E-49 | -1.69E-46 |
| MS.gene058723 | MS.gene85923 | 0.877486 | 6.05E-69 | -1.69E-46 |
| MS.gene058773 | MS.gene85923 | 0.889772 | 1.80E-73 | -1.69E-46 |
| MS.gene058838 | MS.gene85923 | 0.833976 | 3.91E-56 | -1.69E-46 |
| MS.gene059022 | MS.gene85923 | 0.865785 | 4.59E-65 | -1.69E-46 |
| MS.gene059023 | MS.gene85923 | 0.864487 | 1.17E-64 | -1.69E-46 |
| MS.gene059029 | MS.gene85923 | 0.88283 | 7.49E-71 | -1.69E-46 |
| MS.gene059079 | MS.gene85923 | 0.822465 | 2.33E-53 | -1.69E-46 |
| MS.gene05912 | MS.gene85923 | 0.825746 | 3.96E-54 | -1.69E-46 |
| MS.gene05913 | MS.gene85923 | 0.878273 | 3.21E-69 | -1.69E-46 |
| MS.gene05914 | MS.gene85923 | 0.811978 | 5.34E-51 | -1.69E-46 |
| MS.gene059305 | MS.gene85923 | 0.804151 | 2.48E-49 | -1.69E-46 |
| MS.gene05934 | MS.gene85923 | 0.872702 | 2.60E-67 | -1.69E-46 |
| MS.gene059536 | MS.gene85923 | 0.847512 | 1.10E-59 | -1.69E-46 |
| MS.gene059676 | MS.gene85923 | 0.802922 | 4.46E-49 | -1.69E-46 |
| MS.gene059677 | MS.gene85923 | 0.888083 | 8.08E-73 | -1.69E-46 |
| MS.gene059692 | MS.gene85923 | 0.802274 | 6.06E-49 | -1.69E-46 |
| MS.gene059695 | MS.gene85923 | 0.801199 | 1.01E-48 | -1.69E-46 |
| MS.gene059718 | MS.gene85923 | 0.874617 | 5.87E-68 | -1.69E-46 |
| MS.gene059756 | MS.gene85923 | 0.837808 | 4.18E-57 | -1.69E-46 |
| MS.gene059801 | MS.gene85923 | 0.822518 | 2.27E-53 | -1.69E-46 |
| MS.gene059858 | MS.gene85923 | 0.83603 | 1.19E-56 | -1.69E-46 |
| MS.gene059865 | MS.gene85923 | 0.847102 | 1.43E-59 | -1.69E-46 |
| MS.gene059902 | MS.gene85923 | 0.877142 | 7.97E-69 | -1.69E-46 |
| MS.gene059960 | MS.gene85923 | 0.877239 | 7.37E-69 | -1.69E-46 |
| MS.gene060193 | MS.gene85923 | 0.835509 | 1.61E-56 | -1.69E-46 |
| MS.gene060341 | MS.gene85923 | 0.832847 | 7.48E-56 | -1.69E-46 |
| MS.gene060703 | MS.gene85923 | 0.857076 | 2.09E-62 | -1.69E-46 |
| MS.gene060727 | MS.gene85923 | 0.813789 | 2.14E-51 | -1.69E-46 |
| MS.gene060728 | MS.gene85923 | 0.812414 | 4.29E-51 | -1.69E-46 |
| MS.gene060762 | MS.gene85923 | 0.88797 | 8.94E-73 | -1.69E-46 |
| MS.gene06077 | MS.gene85923 | 0.822224 | 2.66E-53 | -1.69E-46 |
| MS.gene060804 | MS.gene85923 | 0.868873 | 4.72E-66 | -1.69E-46 |
| MS.gene060818 | MS.gene85923 | 0.880829 | 3.98E-70 | -1.69E-46 |
| MS.gene060832 | MS.gene85923 | 0.836675 | 8.14E-57 | -1.69E-46 |
| MS.gene060835 | MS.gene85923 | 0.800251 | 1.57E-48 | -1.69E-46 |
| MS.gene060837 | MS.gene85923 | 0.810362 | 1.20E-50 | -1.69E-46 |
| MS.gene06092 | MS.gene85923 | 0.840245 | 9.77E-58 | -1.69E-46 |
| MS.gene060981 | MS.gene85923 | 0.806065 | 9.86E-50 | -1.69E-46 |
| MS.gene060982 | MS.gene85923 | 0.831588 | 1.53E-55 | -1.69E-46 |
| MS.gene061041 | MS.gene85923 | 0.857834 | 1.25E-62 | -1.69E-46 |
| MS.gene061134 | MS.gene85923 | 0.82229 | 2.56E-53 | -1.69E-46 |
| MS.gene061150 | MS.gene85923 | 0.883371 | 4.75E-71 | -1.69E-46 |
| MS.gene061200 | MS.gene85923 | 0.875713 | 2.48E-68 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene85923.t1 | MTR_5g043580 | 98.295 | 176 | 3 | 0 | 1 | 176 | 1 | 176 | 1.92e-128 | 357 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene85923.t1 | AT2G03420 | 73.585 | 106 | 28 | 0 | 69 | 174 | 63 | 168 | 1.00e-53 | 168 |
Find 36 sgRNAs with CRISPR-Local
Find 87 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTGCTTACGAGGGGCGGAAT+GGG | 0.329826 | 5.3:-31051912 | MS.gene85923:CDS |
| CTTCTATTGCAATTCTTCTC+TGG | 0.348974 | 5.3:-31053286 | MS.gene85923:CDS |
| ATGAATTTGTCCCTTGCCTC+TGG | 0.354711 | 5.3:+31051857 | None:intergenic |
| CCTCAAAAGAAGGTTCAGTT+TGG | 0.387035 | 5.3:-31053395 | MS.gene85923:CDS |
| TGAAACCATTAAAACGTTTG+AGG | 0.439460 | 5.3:+31052746 | None:intergenic |
| AAGTCAATCTGAGGCAGCTC+AGG | 0.441780 | 5.3:+31052769 | None:intergenic |
| ACAGGACTAGAGTCTGTTCC+TGG | 0.456712 | 5.3:-31052793 | MS.gene85923:CDS |
| GTAAGAATGATGAGAAGATA+AGG | 0.480601 | 5.3:+31053340 | None:intergenic |
| CGAGGGGCGGAATGGGGAGT+TGG | 0.490686 | 5.3:-31051905 | MS.gene85923:CDS |
| ACTGCTTACGAGGGGCGGAA+TGG | 0.499290 | 5.3:-31051913 | MS.gene85923:CDS |
| GCTCAAGCAGTTTCTTCACC+AGG | 0.505465 | 5.3:+31052578 | None:intergenic |
| CATAGCAATGTTGAAGGAGA+AGG | 0.511435 | 5.3:-31051837 | MS.gene85923:CDS |
| GCACATGCTGGATTCATGTC+AGG | 0.519014 | 5.3:-31052820 | MS.gene85923:CDS |
| ATCTGAGGCAGCTCAGGACC+AGG | 0.523597 | 5.3:+31052775 | None:intergenic |
| CAAATTCATAGCAATGTTGA+AGG | 0.527626 | 5.3:-31051843 | MS.gene85923:CDS |
| ACTCAGCAATCCTCAAAAGA+AGG | 0.529490 | 5.3:-31053405 | MS.gene85923:CDS |
| ATTCTTCTCTGGTCAACTCC+AGG | 0.534134 | 5.3:-31053275 | MS.gene85923:intron |
| GTGGAAATCTGAATGAAAGA+TGG | 0.538239 | 5.3:+31053440 | None:intergenic |
| CTCAAGCAGTTTCTTCACCA+GGG | 0.543128 | 5.3:+31052579 | None:intergenic |
| GGGAATGACTCCAGAGGCAA+GGG | 0.550474 | 5.3:-31051867 | MS.gene85923:CDS |
| GGATTCATGTCAGGAATCAC+AGG | 0.551319 | 5.3:-31052811 | MS.gene85923:CDS |
| TGCTTACGAGGGGCGGAATG+GGG | 0.568619 | 5.3:-31051911 | MS.gene85923:CDS |
| AATGAAAGATGGTAATGAAG+AGG | 0.576236 | 5.3:+31053451 | None:intergenic |
| AGGGAATGACTCCAGAGGCA+AGG | 0.577514 | 5.3:-31051868 | MS.gene85923:CDS |
| GAGAACAAGTACTGCTTACG+AGG | 0.584541 | 5.3:-31051923 | MS.gene85923:CDS |
| GTTGGTGATTGTTCAGCAGA+GGG | 0.594398 | 5.3:-31051887 | MS.gene85923:CDS |
| TCAAGCAGTTTCTTCACCAG+GGG | 0.603793 | 5.3:+31052580 | None:intergenic |
| AGTTGGTGATTGTTCAGCAG+AGG | 0.606424 | 5.3:-31051888 | MS.gene85923:CDS |
| CAAGTACTGCTTACGAGGGG+CGG | 0.612792 | 5.3:-31051918 | MS.gene85923:CDS |
| GCAATGTTGAAGGAGAAGGT+TGG | 0.630694 | 5.3:-31051833 | MS.gene85923:CDS |
| CGTTTGAGGAAGTCAATCTG+AGG | 0.656409 | 5.3:+31052760 | None:intergenic |
| AAGATTTAAAGAGACTCCCC+TGG | 0.658995 | 5.3:-31052596 | MS.gene85923:CDS |
| AGAACAAGTACTGCTTACGA+GGG | 0.671236 | 5.3:-31051922 | MS.gene85923:CDS |
| ATTGCAATAGAAGCAGCCAA+AGG | 0.674848 | 5.3:+31053296 | None:intergenic |
| AGCAGAGGGAATGACTCCAG+AGG | 0.703314 | 5.3:-31051873 | MS.gene85923:CDS |
| GAACAAGTACTGCTTACGAG+GGG | 0.743478 | 5.3:-31051921 | MS.gene85923:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TAATGTCCAATTAAAAAAAT+TGG | - | chr5.3:31052918-31052937 | MS.gene85923:intron | 15.0% |
| !! | TTTGAATGGTTAATATATTT+CGG | - | chr5.3:31053168-31053187 | MS.gene85923:intron | 15.0% |
| !! | AAATTGTGTATGAGAAATAT+TGG | + | chr5.3:31052877-31052896 | None:intergenic | 20.0% |
| !! | AATACATCATATAAGTAACT+AGG | - | chr5.3:31053029-31053048 | MS.gene85923:intron | 20.0% |
| !! | AATATTTCTCATACACAATT+TGG | - | chr5.3:31052876-31052895 | MS.gene85923:intron | 20.0% |
| !! | AATGTTTCACTTTAATTCTT+AGG | + | chr5.3:31052351-31052370 | None:intergenic | 20.0% |
| !! | AGTTTCATTTCATGAAATTT+AGG | + | chr5.3:31052598-31052617 | None:intergenic | 20.0% |
| !! | ATTGACAAAAATGTAAGAAT+TGG | - | chr5.3:31053082-31053101 | MS.gene85923:intron | 20.0% |
| !! | TTATGATTATTATTTCACAG+AGG | - | chr5.3:31052631-31052650 | MS.gene85923:CDS | 20.0% |
| ! | ACCATTCAAAAATAAAGTCA+TGG | + | chr5.3:31053158-31053177 | None:intergenic | 25.0% |
| ! | CTCACAAAAAAGAATATTAC+AGG | + | chr5.3:31053121-31053140 | None:intergenic | 25.0% |
| ! | CTTTAATTCTTAGGAATCAT+AGG | + | chr5.3:31052342-31052361 | None:intergenic | 25.0% |
| ! | TAGCCTAAAAAAAAGATAAG+AGG | + | chr5.3:31053004-31053023 | None:intergenic | 25.0% |
| ! | TCCAATTAAAAAAATTGGCT+CGG | - | chr5.3:31052923-31052942 | MS.gene85923:intron | 25.0% |
| ! | TTAATACCAAGACGAATAAT+AGG | + | chr5.3:31053248-31053267 | None:intergenic | 25.0% |
| !! | TAAGATAGTTTGAGTTAGTT+AGG | - | chr5.3:31052217-31052236 | MS.gene85923:intron | 25.0% |
| !! | TTGATTATGATTTGATGACA+CGG | + | chr5.3:31052160-31052179 | None:intergenic | 25.0% |
| !! | TTTTGTCAATATAAGAAGAC+AGG | + | chr5.3:31053072-31053091 | None:intergenic | 25.0% |
| !!! | ACCATGACTTTATTTTTGAA+TGG | - | chr5.3:31053154-31053173 | MS.gene85923:intron | 25.0% |
| !!! | ATTTCTGTGTTTTATTCTTC+AGG | - | chr5.3:31053320-31053339 | MS.gene85923:CDS | 25.0% |
| ACATATCAACATAGCACTTT+AGG | + | chr5.3:31052837-31052856 | None:intergenic | 30.0% | |
| ATATGAGATAGAGATAGCAT+AGG | + | chr5.3:31052295-31052314 | None:intergenic | 30.0% | |
| CAAATTCATAGCAATGTTGA+AGG | - | chr5.3:31053438-31053457 | MS.gene85923:CDS | 30.0% | |
| CATATCAACATAGCACTTTA+GGG | + | chr5.3:31052836-31052855 | None:intergenic | 30.0% | |
| CTACATTAAAGCATGGTAAA+AGG | + | chr5.3:31052040-31052059 | None:intergenic | 30.0% | |
| GTAAGAATGATGAGAAGATA+AGG | + | chr5.3:31051944-31051963 | None:intergenic | 30.0% | |
| TCTTCAAAAATCAGTTCCTT+TGG | - | chr5.3:31051969-31051988 | MS.gene85923:intron | 30.0% | |
| TGAAACCATTAAAACGTTTG+AGG | + | chr5.3:31052538-31052557 | None:intergenic | 30.0% | |
| TTAGATCCTATTATTCGTCT+TGG | - | chr5.3:31053239-31053258 | MS.gene85923:intron | 30.0% | |
| ! | AAAGTGCTATGTTGATATGT+CGG | - | chr5.3:31052837-31052856 | MS.gene85923:intron | 30.0% |
| ! | AATGAAAGATGGTAATGAAG+AGG | + | chr5.3:31051833-31051852 | None:intergenic | 30.0% |
| ! | CATTTTCAGCATATTTCTTC+TGG | + | chr5.3:31052661-31052680 | None:intergenic | 30.0% |
| !!! | GAGCCTCTTATCTTTTTTTT+AGG | - | chr5.3:31052998-31053017 | MS.gene85923:intron | 30.0% |
| !!! | TCCGAGCCAATTTTTTTAAT+TGG | + | chr5.3:31052927-31052946 | None:intergenic | 30.0% |
| ATAAGAGGCTCAATAGATGT+TGG | + | chr5.3:31052989-31053008 | None:intergenic | 35.0% | |
| ATAGGATCTAATGCATACAC+CGG | + | chr5.3:31053230-31053249 | None:intergenic | 35.0% | |
| CTTCTATTGCAATTCTTCTC+TGG | - | chr5.3:31051995-31052014 | MS.gene85923:intron | 35.0% | |
| GTGGAAATCTGAATGAAAGA+TGG | + | chr5.3:31051844-31051863 | None:intergenic | 35.0% | |
| TGACGATTATGATTGATCGA+CGG | - | chr5.3:31052381-31052400 | MS.gene85923:intron | 35.0% | |
| TTCATTAGAGACACATGCTA+AGG | - | chr5.3:31052082-31052101 | MS.gene85923:intron | 35.0% | |
| TTGCATGCTACATTAAAGCA+TGG | + | chr5.3:31052047-31052066 | None:intergenic | 35.0% | |
| ! | ATTGCTGAGTTTTTCACAGA+AGG | + | chr5.3:31051866-31051885 | None:intergenic | 35.0% |
| ! | GACTTCCTCAAACGTTTTAA+TGG | - | chr5.3:31052530-31052549 | MS.gene85923:intron | 35.0% |
| ! | TGGTAAAAGGTTACTTTACC+TGG | + | chr5.3:31052027-31052046 | None:intergenic | 35.0% |
| ! | TTGATATGTCGGATACGTTT+TGG | - | chr5.3:31052848-31052867 | MS.gene85923:intron | 35.0% |
| AAGATTTAAAGAGACTCCCC+TGG | - | chr5.3:31052685-31052704 | MS.gene85923:intron | 40.0% | |
| ACTCAGCAATCCTCAAAAGA+AGG | - | chr5.3:31051876-31051895 | MS.gene85923:CDS | 40.0% | |
| ATCTAATGCATACACCGGAT+AGG | + | chr5.3:31053225-31053244 | None:intergenic | 40.0% | |
| ATTGCAATAGAAGCAGCCAA+AGG | + | chr5.3:31051988-31052007 | None:intergenic | 40.0% | |
| CATAGCAATGTTGAAGGAGA+AGG | - | chr5.3:31053444-31053463 | MS.gene85923:CDS | 40.0% | |
| CCTCAAAAGAAGGTTCAGTT+TGG | - | chr5.3:31051886-31051905 | MS.gene85923:CDS | 40.0% | |
| GTGTCCAAGATATGTCTAAC+AGG | + | chr5.3:31052950-31052969 | None:intergenic | 40.0% | |
| ! | AGAACAAGTACTGCTTACGA+GGG | - | chr5.3:31053359-31053378 | MS.gene85923:CDS | 40.0% |
| ! | CCAAACTGAACCTTCTTTTG+AGG | + | chr5.3:31051889-31051908 | None:intergenic | 40.0% |
| !!! | TCTTATTTTGCAGCACATGC+TGG | - | chr5.3:31052449-31052468 | MS.gene85923:intron | 40.0% |
| ATGAATTTGTCCCTTGCCTC+TGG | + | chr5.3:31053427-31053446 | None:intergenic | 45.0% | |
| ATTCTTCTCTGGTCAACTCC+AGG | - | chr5.3:31052006-31052025 | MS.gene85923:intron | 45.0% | |
| CGGACCTGTTAGACATATCT+TGG | - | chr5.3:31052943-31052962 | MS.gene85923:intron | 45.0% | |
| CGTTTGAGGAAGTCAATCTG+AGG | + | chr5.3:31052524-31052543 | None:intergenic | 45.0% | |
| CTCAAGCAGTTTCTTCACCA+GGG | + | chr5.3:31052705-31052724 | None:intergenic | 45.0% | |
| GCAATGTTGAAGGAGAAGGT+TGG | - | chr5.3:31053448-31053467 | MS.gene85923:CDS | 45.0% | |
| GGATTCATGTCAGGAATCAC+AGG | - | chr5.3:31052470-31052489 | MS.gene85923:intron | 45.0% | |
| GTGTCTCTCTTTGTCCTATC+CGG | - | chr5.3:31053208-31053227 | MS.gene85923:intron | 45.0% | |
| TCAAGCAGTTTCTTCACCAG+GGG | + | chr5.3:31052704-31052723 | None:intergenic | 45.0% | |
| ! | AGGCTCAATAGATGTTGGCT+CGG | + | chr5.3:31052984-31053003 | None:intergenic | 45.0% |
| ! | CAACATAGCACTTTAGGGAG+AGG | + | chr5.3:31052831-31052850 | None:intergenic | 45.0% |
| ! | GAACAAGTACTGCTTACGAG+GGG | - | chr5.3:31053360-31053379 | MS.gene85923:CDS | 45.0% |
| ! | GAGAACAAGTACTGCTTACG+AGG | - | chr5.3:31053358-31053377 | MS.gene85923:CDS | 45.0% |
| ! | GCTGAGTTTTTCACAGAAGG+TGG | + | chr5.3:31051863-31051882 | None:intergenic | 45.0% |
| !! | AGTTGGTGATTGTTCAGCAG+AGG | - | chr5.3:31053393-31053412 | MS.gene85923:CDS | 45.0% |
| !! | GTTGGTGATTGTTCAGCAGA+GGG | - | chr5.3:31053394-31053413 | MS.gene85923:CDS | 45.0% |
| ACAGGACTAGAGTCTGTTCC+TGG | - | chr5.3:31052488-31052507 | MS.gene85923:intron | 50.0% | |
| CAAAGAGAGACACCGCAACA+CGG | + | chr5.3:31053201-31053220 | None:intergenic | 50.0% | |
| GCACATGCTGGATTCATGTC+AGG | - | chr5.3:31052461-31052480 | MS.gene85923:intron | 50.0% | |
| GCTCAAGCAGTTTCTTCACC+AGG | + | chr5.3:31052706-31052725 | None:intergenic | 50.0% | |
| ! | AAGTCAATCTGAGGCAGCTC+AGG | + | chr5.3:31052515-31052534 | None:intergenic | 50.0% |
| AGCAGAGGGAATGACTCCAG+AGG | - | chr5.3:31053408-31053427 | MS.gene85923:CDS | 55.0% | |
| AGGGAATGACTCCAGAGGCA+AGG | - | chr5.3:31053413-31053432 | MS.gene85923:CDS | 55.0% | |
| GGGAATGACTCCAGAGGCAA+GGG | - | chr5.3:31053414-31053433 | MS.gene85923:CDS | 55.0% | |
| GGTGTCTGTGCTGTGCTCAT+AGG | + | chr5.3:31052810-31052829 | None:intergenic | 55.0% | |
| TTCGGACACAAGCCGTGTTG+CGG | - | chr5.3:31053186-31053205 | MS.gene85923:intron | 55.0% | |
| ! | CAAGTACTGCTTACGAGGGG+CGG | - | chr5.3:31053363-31053382 | MS.gene85923:CDS | 55.0% |
| ACTGCTTACGAGGGGCGGAA+TGG | - | chr5.3:31053368-31053387 | MS.gene85923:CDS | 60.0% | |
| CTGCTTACGAGGGGCGGAAT+GGG | - | chr5.3:31053369-31053388 | MS.gene85923:CDS | 60.0% | |
| TGCTTACGAGGGGCGGAATG+GGG | - | chr5.3:31053370-31053389 | MS.gene85923:CDS | 60.0% | |
| ! | ATCTGAGGCAGCTCAGGACC+AGG | + | chr5.3:31052509-31052528 | None:intergenic | 60.0% |
| CGAGGGGCGGAATGGGGAGT+TGG | - | chr5.3:31053376-31053395 | MS.gene85923:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.3 | gene | 31051823 | 31053480 | 31051823 | ID=MS.gene85923 |
| chr5.3 | mRNA | 31051823 | 31053480 | 31051823 | ID=MS.gene85923.t1;Parent=MS.gene85923 |
| chr5.3 | exon | 31053276 | 31053480 | 31053276 | ID=MS.gene85923.t1.exon1;Parent=MS.gene85923.t1 |
| chr5.3 | CDS | 31053276 | 31053480 | 31053276 | ID=cds.MS.gene85923.t1;Parent=MS.gene85923.t1 |
| chr5.3 | exon | 31052752 | 31052841 | 31052752 | ID=MS.gene85923.t1.exon2;Parent=MS.gene85923.t1 |
| chr5.3 | CDS | 31052752 | 31052841 | 31052752 | ID=cds.MS.gene85923.t1;Parent=MS.gene85923.t1 |
| chr5.3 | exon | 31052556 | 31052652 | 31052556 | ID=MS.gene85923.t1.exon3;Parent=MS.gene85923.t1 |
| chr5.3 | CDS | 31052556 | 31052652 | 31052556 | ID=cds.MS.gene85923.t1;Parent=MS.gene85923.t1 |
| chr5.3 | exon | 31051823 | 31051961 | 31051823 | ID=MS.gene85923.t1.exon4;Parent=MS.gene85923.t1 |
| chr5.3 | CDS | 31051823 | 31051961 | 31051823 | ID=cds.MS.gene85923.t1;Parent=MS.gene85923.t1 |
| Gene Sequence |
| Protein sequence |
