AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene068917


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene068917.t1 MTR_2g045080 90.643 171 6 1 1 161 1 171 4.75e-112 315
MS.gene068917.t1 MTR_2g045050 76.398 161 38 0 1 161 1 161 2.61e-92 265
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene068917.t1 AT1G48320 63.462 156 55 1 6 161 3 156 1.41e-69 207
MS.gene068917.t1 AT5G48950 54.667 150 65 2 11 157 3 152 8.71e-58 177
MS.gene068917.t1 AT5G48950 60.185 108 41 1 11 116 3 110 4.26e-43 139

Find 21 sgRNAs with CRISPR-Local

Find 85 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATTTACCCCTTCATACATT+TGG 0.265244 2.1:-45899450 MS.gene068917:CDS
AGCATTCACCTGAATGGTTT+TGG 0.290561 2.1:+45898727 None:intergenic
GTGTCATCAAACAAAGTTTC+TGG 0.297801 2.1:-45899411 MS.gene068917:CDS
TGCATACTTCTTTAGCATAT+TGG 0.303673 2.1:+45898081 None:intergenic
AAAACCAAAAGCTGCTGATT+TGG 0.309085 2.1:-45899472 MS.gene068917:CDS
CCTCCATGAAGCACACTAAA+AGG 0.309265 2.1:+45898905 None:intergenic
AAGTGTTGCCAAAACCATTC+AGG 0.313827 2.1:-45898735 MS.gene068917:intron
AAATCCAAATCAGCAGCTTT+TGG 0.333199 2.1:+45899468 None:intergenic
ATTTACCCCTTCATACATTT+GGG 0.334094 2.1:-45899449 MS.gene068917:CDS
TGTGGGTATAAAAGAGTTGC+TGG 0.445165 2.1:-45898830 MS.gene068917:CDS
AAGAGGAGCATTCACCTGAA+TGG 0.482948 2.1:+45898721 None:intergenic
GCTGAAGCTCTTGCAAGTAT+TGG 0.487992 2.1:-45898866 MS.gene068917:CDS
AGGTGTGGGATGTGAAAATC+TGG 0.507075 2.1:-45898204 MS.gene068917:CDS
ACTCAAACCCAAATGTATGA+AGG 0.525757 2.1:+45899442 None:intergenic
TTGGAGCACATGTTGCTTGT+GGG 0.528993 2.1:-45898847 MS.gene068917:CDS
CTCAAACCCAAATGTATGAA+GGG 0.530132 2.1:+45899443 None:intergenic
CATTTGAAATCAGCAATGCT+TGG 0.536764 2.1:-45898788 MS.gene068917:CDS
AGTCACTGAAAAGTGTTGCC+AGG 0.553880 2.1:-45899379 MS.gene068917:intron
GGAGGACCATCAATCATCAT+CGG 0.605735 2.1:-45899496 MS.gene068917:CDS
ATTGGAGCACATGTTGCTTG+TGG 0.616187 2.1:-45898848 MS.gene068917:CDS
TCAAACCCAAATGTATGAAG+GGG 0.634737 2.1:+45899444 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATAAGAGAATTTATATAAA+TGG + chr2.1:45899001-45899020 None:intergenic 10.0%
!! ATTTATATAAATTCTCTTAT+TGG - chr2.1:45899000-45899019 MS.gene068917:intron 10.0%
!!! ATATCATCTCATATTTATTT+TGG - chr2.1:45899091-45899110 MS.gene068917:intron 15.0%
!! ATATATGAACAAGAAAAAGT+AGG - chr2.1:45898609-45898628 MS.gene068917:intron 20.0%
!!! ACTTTGAGAAAAATATAAAG+AGG + chr2.1:45898869-45898888 None:intergenic 20.0%
!!! TTTACTTGCTTACATTTTTT+GGG - chr2.1:45899290-45899309 MS.gene068917:intron 20.0%
!!! TTTTACTTGCTTACATTTTT+TGG - chr2.1:45899289-45899308 MS.gene068917:intron 20.0%
! AATTAGGAGTGATTTGATAT+TGG - chr2.1:45898633-45898652 MS.gene068917:intron 25.0%
! ACAAGAAAAAGTAGGTAATT+AGG - chr2.1:45898617-45898636 MS.gene068917:intron 25.0%
! ACGAAAAACTATGAAAACAA+AGG + chr2.1:45898223-45898242 None:intergenic 25.0%
! ATCATGATTGGTATAATCAT+TGG + chr2.1:45899158-45899177 None:intergenic 25.0%
! CACATAAAGATAAATCGTTT+GGG + chr2.1:45898931-45898950 None:intergenic 25.0%
! CGAAAAACTATGAAAACAAA+GGG + chr2.1:45898222-45898241 None:intergenic 25.0%
! CGGATTCTGATTTAAATAAT+TGG + chr2.1:45898896-45898915 None:intergenic 25.0%
! GACAATGCATAAAATATGTA+AGG - chr2.1:45898358-45898377 MS.gene068917:intron 25.0%
! GCTATAAAGTAAGTAAAACA+TGG - chr2.1:45899132-45899151 MS.gene068917:intron 25.0%
! TCACATAAAGATAAATCGTT+TGG + chr2.1:45898932-45898951 None:intergenic 25.0%
! TTGAGTATGTTACATAGTTA+TGG - chr2.1:45898553-45898572 MS.gene068917:intron 25.0%
!! AAATTCTCTTATTGGTGATT+TGG - chr2.1:45899008-45899027 MS.gene068917:intron 25.0%
!! TGTTTTACTTACTTTATAGC+CGG + chr2.1:45899132-45899151 None:intergenic 25.0%
!!! CTCATATTTATTTTGGTACT+TGG - chr2.1:45899098-45899117 MS.gene068917:intron 25.0%
!!! TTACTTGCTTACATTTTTTG+GGG - chr2.1:45899291-45899310 MS.gene068917:intron 25.0%
AATTAAGCACATATACTCCT+TGG - chr2.1:45898287-45898306 MS.gene068917:intron 30.0%
ATTTACCCCTTCATACATTT+GGG - chr2.1:45898121-45898140 MS.gene068917:CDS 30.0%
TGCATAAAATATGTAAGGTC+CGG - chr2.1:45898363-45898382 MS.gene068917:intron 30.0%
TGCATACTTCTTTAGCATAT+TGG + chr2.1:45899492-45899511 None:intergenic 30.0%
TTGAAGACAATGTCACTAAT+TGG - chr2.1:45898966-45898985 MS.gene068917:intron 30.0%
!!! GTTCTGTTTTTTGAGTTTGA+AGG + chr2.1:45899401-45899420 None:intergenic 30.0%
!!! TTTTGGGGTTAGAATTGTTT+GGG - chr2.1:45899306-45899325 MS.gene068917:intron 30.0%
!!! TTTTTGGGGTTAGAATTGTT+TGG - chr2.1:45899305-45899324 MS.gene068917:intron 30.0%
AAAGAGACATGATCATGTGT+TGG - chr2.1:45898409-45898428 MS.gene068917:intron 35.0%
ACAATGTCACTAATTGGCTA+TGG - chr2.1:45898972-45898991 MS.gene068917:intron 35.0%
ACATGATCATGTCTAATCCA+AGG + chr2.1:45898307-45898326 None:intergenic 35.0%
ACTCAAACCCAAATGTATGA+AGG + chr2.1:45898131-45898150 None:intergenic 35.0%
CATAAAATATGTAAGGTCCG+GGG - chr2.1:45898365-45898384 MS.gene068917:intron 35.0%
CTCAAACCCAAATGTATGAA+GGG + chr2.1:45898130-45898149 None:intergenic 35.0%
GATTTACCCCTTCATACATT+TGG - chr2.1:45898120-45898139 MS.gene068917:CDS 35.0%
GCATAAAATATGTAAGGTCC+GGG - chr2.1:45898364-45898383 MS.gene068917:intron 35.0%
GTGTCATCAAACAAAGTTTC+TGG - chr2.1:45898159-45898178 MS.gene068917:CDS 35.0%
TCAAACCCAAATGTATGAAG+GGG + chr2.1:45898129-45898148 None:intergenic 35.0%
! AAAACCAAAAGCTGCTGATT+TGG - chr2.1:45898098-45898117 MS.gene068917:CDS 35.0%
! AAATCCAAATCAGCAGCTTT+TGG + chr2.1:45898105-45898124 None:intergenic 35.0%
! ACTTTTCAGTGACTTGAAGA+TGG + chr2.1:45898185-45898204 None:intergenic 35.0%
! ATGATCATGTCTCTTTTTGG+TGG + chr2.1:45898405-45898424 None:intergenic 35.0%
! CACATGATCATGTCTCTTTT+TGG + chr2.1:45898408-45898427 None:intergenic 35.0%
! CATTTGAAATCAGCAATGCT+TGG - chr2.1:45898782-45898801 MS.gene068917:CDS 35.0%
!! TCTTTTGCATAATCTGGAAC+AGG + chr2.1:45899464-45899483 None:intergenic 35.0%
!!! ATGGTTTTGGCAACACTTAA+AGG + chr2.1:45898833-45898852 None:intergenic 35.0%
!!! TGGTTTTGGCAACACTTAAA+GGG + chr2.1:45898832-45898851 None:intergenic 35.0%
ACACGAACACCAACAAAACA+CGG + chr2.1:45898533-45898552 None:intergenic 40.0%
AGTTGAGCTCGAATCATGAT+TGG + chr2.1:45899170-45899189 None:intergenic 40.0%
CGTTTGGGAAAACTTGAGTA+CGG + chr2.1:45898916-45898935 None:intergenic 40.0%
GATATGTGTCAGTATCACGT+CGG + chr2.1:45898454-45898473 None:intergenic 40.0%
TGAAAACAAAGGGTTTGACC+TGG + chr2.1:45898212-45898231 None:intergenic 40.0%
TGTGGGTATAAAAGAGTTGC+TGG - chr2.1:45898740-45898759 MS.gene068917:CDS 40.0%
TTACACTTTGATCCTGTTGC+AGG - chr2.1:45899346-45899365 MS.gene068917:intron 40.0%
! AAGTGTTGCCAAAACCATTC+AGG - chr2.1:45898835-45898854 MS.gene068917:CDS 40.0%
! AGCATTCACCTGAATGGTTT+TGG + chr2.1:45898846-45898865 None:intergenic 40.0%
! CTTTTAGTGTGCTTCATGGA+GGG - chr2.1:45898666-45898685 MS.gene068917:intron 40.0%
! GCAGCTTCTTTTGCATAATC+TGG + chr2.1:45899470-45899489 None:intergenic 40.0%
! GTTTTGCCGATGATGATTGA+TGG + chr2.1:45898083-45898102 None:intergenic 40.0%
! TGAGCTTAGTTCAGTTGGTA+TGG - chr2.1:45898336-45898355 MS.gene068917:intron 40.0%
! TGTCTATGTCCGTGTTTTGT+TGG - chr2.1:45898521-45898540 MS.gene068917:intron 40.0%
! TTTTAGTGTGCTTCATGGAG+GGG - chr2.1:45898667-45898686 MS.gene068917:intron 40.0%
!! GGTTTTGGCAACACTTAAAG+GGG + chr2.1:45898831-45898850 None:intergenic 40.0%
AGGTGTGGGATGTGAAAATC+TGG - chr2.1:45899366-45899385 MS.gene068917:intron 45.0%
CCTCCATGAAGCACACTAAA+AGG + chr2.1:45898668-45898687 None:intergenic 45.0%
GCTGAAGCTCTTGCAAGTAT+TGG - chr2.1:45898704-45898723 MS.gene068917:intron 45.0%
GGAGGACCATCAATCATCAT+CGG - chr2.1:45898074-45898093 MS.gene068917:CDS 45.0%
TGGGGTTAGAATTGTTTGGG+CGG - chr2.1:45899309-45899328 MS.gene068917:intron 45.0%
TTTGATCCTGTTGCAGGTGT+GGG - chr2.1:45899352-45899371 MS.gene068917:intron 45.0%
! ATTGGAGCACATGTTGCTTG+TGG - chr2.1:45898722-45898741 MS.gene068917:intron 45.0%
! CAGCCTTTTAGTGTGCTTCA+TGG - chr2.1:45898662-45898681 MS.gene068917:intron 45.0%
! CCTTTTAGTGTGCTTCATGG+AGG - chr2.1:45898665-45898684 MS.gene068917:intron 45.0%
! TTGGAGCACATGTTGCTTGT+GGG - chr2.1:45898723-45898742 MS.gene068917:intron 45.0%
!! AAGAGGAGCATTCACCTGAA+TGG + chr2.1:45898852-45898871 None:intergenic 45.0%
!! AGTCACTGAAAAGTGTTGCC+AGG - chr2.1:45898191-45898210 MS.gene068917:CDS 45.0%
CCAACTGAACTAAGCTCACG+AGG + chr2.1:45898334-45898353 None:intergenic 50.0%
CCTCGTGAGCTTAGTTCAGT+TGG - chr2.1:45898331-45898350 MS.gene068917:intron 50.0%
CTTTGATCCTGTTGCAGGTG+TGG - chr2.1:45899351-45899370 MS.gene068917:intron 50.0%
! TTGGTACTTGGCTCGATGTC+CGG - chr2.1:45899110-45899129 MS.gene068917:intron 50.0%
!!! GTCTCTTTTTGGTGGTGACC+AGG + chr2.1:45898397-45898416 None:intergenic 50.0%
TCACATCCCACACCTGCAAC+AGG + chr2.1:45899361-45899380 None:intergenic 55.0%
TAAGGTCCGGGGTTCGAACC+TGG - chr2.1:45898376-45898395 MS.gene068917:intron 60.0%
TGGTGACCAGGTTCGAACCC+CGG + chr2.1:45898385-45898404 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr2.1 gene 45898072 45899520 45898072 ID=MS.gene068917
chr2.1 mRNA 45898072 45899520 45898072 ID=MS.gene068917.t1;Parent=MS.gene068917
chr2.1 exon 45899380 45899520 45899380 ID=MS.gene068917.t1.exon1;Parent=MS.gene068917.t1
chr2.1 CDS 45899380 45899520 45899380 ID=cds.MS.gene068917.t1;Parent=MS.gene068917.t1
chr2.1 exon 45898736 45898927 45898736 ID=MS.gene068917.t1.exon2;Parent=MS.gene068917.t1
chr2.1 CDS 45898736 45898927 45898736 ID=cds.MS.gene068917.t1;Parent=MS.gene068917.t1
chr2.1 exon 45898072 45898224 45898072 ID=MS.gene068917.t1.exon3;Parent=MS.gene068917.t1
chr2.1 CDS 45898072 45898224 45898072 ID=cds.MS.gene068917.t1;Parent=MS.gene068917.t1
Gene Sequence

>MS.gene068917

ATGGAGGACCATCAATCATCATCGGCAAAACCAAAAGCTGCTGATTTGGATTTACCCCTTCATACATTTGGGTTTGAGTTTCAAGATGTGTCATCAAACAAAGTTTCTGGCCATCTTCAAGTCACTGAAAAGTGTTGCCAGGTCAAACCCTTTGTTTTCATAGTTTTTCGTCTCTTAATTCTTATAACAATTATTTGATACTATGATTCTTGAAAAATTAAGCACATATACTCCTTGGATTAGACATGATCATGTGTGTCCTCGTGAGCTTAGTTCAGTTGGTATGGACAATGCATAAAATATGTAAGGTCCGGGGTTCGAACCTGGTCACCACCAAAAAGAGACATGATCATGTGTTGGATATGTAACATGTCTGACACCGACGTGATACTGACACATATCATTATATTGAATTATGTTATTTTCTCAAATTATTATCAGCTTTGACGTGTCTATGTCCGTGTTTTGTTGGTGTTCGTGTTTGAGTATGTTACATAGTTATGGAATCATATTATTTTTTATATTAATTTCTCACATATATATGAACAAGAAAAAGTAGGTAATTAGGAGTGATTTGATATTGGATGTTGCAGCCTTTTAGTGTGCTTCATGGAGGGGTATCAGCAGTGATAGCTGAAGCTCTTGCAAGTATTGGAGCACATGTTGCTTGTGGGTATAAAAGAGTTGCTGGAATTCAACTTAGCATCAATCATTTGAAATCAGCAATGCTTGGTGACTTGATCTATGCTGAAGCAACCCCTTTAAGTGTTGCCAAAACCATTCAGGTGAATGCTCCTCTTTATATTTTTCTCAAAGTCTGACCAATTATTTAAATCAGAATCCGTACTCAAGTTTTCCCAAACGATTTATCTTTATGTGAAGCACACTATATAATTGAAGACAATGTCACTAATTGGCTATGGTTACCATTTATATAAATTCTCTTATTGGTGATTTGGCGCATAATAATAACAATAGAACATAAGACATACTCTAAGATGTTTTTGTAATTTTATTAGATATCATCTCATATTTATTTTGGTACTTGGCTCGATGTCCGGCTATAAAGTAAGTAAAACATGGCCAATGATTATACCAATCATGATTCGAGCTCAACTCTCTTAAACAATTCGTCTTTAACTGAAGTTCATTAATGACTTGAGTATAATCATTTGTTTGAATCATCTCATTGTTTACTACAAATGTTGTGTTTAAATTTTTACTTGCTTACATTTTTTGGGGTTAGAATTGTTTGGGCGGATATGATCGTTATCTTACACTTTGATCCTGTTGCAGGTGTGGGATGTGAAAATCTGGAAGATCGATCCTTCAAACTCAAAAAACAGAACGTTGATAGCATATTCTAGAGTAACTCTTAAAAGCAACATGCCTGTTCCAGATTATGCAAAAGAAGCTGCCAATATGCTAAAGAAGTATGCAAAGCTGTAA

Protein sequence

>MS.gene068917.t1

MEDHQSSSAKPKAADLDLPLHTFGFEFQDVSSNKVSGHLQVTEKCCQPFSVLHGGVSAVIAEALASIGAHVACGYKRVAGIQLSINHLKSAMLGDLIYAEATPLSVAKTIQVWDVKIWKIDPSNSKNRTLIAYSRVTLKSNMPVPDYAKEAANMLKKYAKL