AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene062071


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene062071.t1 MTR_2g045080 90.058 171 7 1 1 161 1 171 2.25e-111 313
MS.gene062071.t1 MTR_2g045050 75.776 161 39 0 1 161 1 161 9.95e-92 263
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene062071.t1 AT1G48320 64.103 156 54 1 6 161 3 156 6.91e-70 208
MS.gene062071.t1 AT5G48950 55.333 150 64 2 11 157 3 152 3.08e-58 179
MS.gene062071.t1 AT5G48950 60.185 108 41 1 11 116 3 110 4.03e-43 139

Find 21 sgRNAs with CRISPR-Local

Find 88 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATTTACCCCTTCATACATT+TGG 0.265244 2.3:-45220171 MS.gene062071:CDS
AGCATTCACCTGAATGGTTT+TGG 0.290561 2.3:+45219448 None:intergenic
GTGTCATCAAACAAAGTTTC+TGG 0.297801 2.3:-45220132 MS.gene062071:CDS
AAAACCAAAAGCTGCTGATT+TGG 0.309085 2.3:-45220193 MS.gene062071:CDS
CCTCCATGAAGCACACTAAA+AGG 0.309265 2.3:+45219626 None:intergenic
AAGTGTTGCCAAAACCATTC+AGG 0.313827 2.3:-45219456 MS.gene062071:intron
AAATCCAAATCAGCAGCTTT+TGG 0.333199 2.3:+45220189 None:intergenic
ATTTACCCCTTCATACATTT+GGG 0.334094 2.3:-45220170 MS.gene062071:CDS
TGCATACTTCTTTAGCATAT+TGG 0.339467 2.3:+45218802 None:intergenic
TGTGGGTATAAAAGAGTTGC+TGG 0.445165 2.3:-45219551 MS.gene062071:CDS
AAGAGGAGCATTCACCTGAA+TGG 0.482948 2.3:+45219442 None:intergenic
GCTGAAGCTCTTGCAAGTAT+TGG 0.487992 2.3:-45219587 MS.gene062071:CDS
AGGTGTGGGATGTGAAAATC+TGG 0.507075 2.3:-45218925 MS.gene062071:CDS
ACTCAAACCCAAATGTATGA+AGG 0.525757 2.3:+45220163 None:intergenic
TTGGAGCACATGTTGCTTGT+GGG 0.528993 2.3:-45219568 MS.gene062071:CDS
CTCAAACCCAAATGTATGAA+GGG 0.530132 2.3:+45220164 None:intergenic
CATTTGAAATCAGCAATGCT+TGG 0.536764 2.3:-45219509 MS.gene062071:CDS
AGTCACTGAAAAGTGTTGCC+AGG 0.553880 2.3:-45220100 MS.gene062071:intron
GGAGGACCATCAATCATCAT+CGG 0.605735 2.3:-45220217 MS.gene062071:CDS
ATTGGAGCACATGTTGCTTG+TGG 0.616187 2.3:-45219569 MS.gene062071:CDS
TCAAACCCAAATGTATGAAG+GGG 0.634737 2.3:+45220165 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATAAGAGAATTTATATAAA+TGG + chr2.3:45219722-45219741 None:intergenic 10.0%
!! ATTTATATAAATTCTCTTAT+TGG - chr2.3:45219721-45219740 MS.gene062071:intron 10.0%
!! AAAAATTTAAACACAACATT+TGG + chr2.3:45219994-45220013 None:intergenic 15.0%
!!! ATATCATCTCATATTTATTT+TGG - chr2.3:45219812-45219831 MS.gene062071:intron 15.0%
!! ATATATGAACAAGAAAAAGT+AGG - chr2.3:45219330-45219349 MS.gene062071:intron 20.0%
!! TTCAATATAATGATATGTGT+CGG + chr2.3:45219186-45219205 None:intergenic 20.0%
!!! ACTTTGAGAAAAATATAAAG+AGG + chr2.3:45219590-45219609 None:intergenic 20.0%
!!! TTTACTTGCTTACATTTTTT+GGG - chr2.3:45220011-45220030 MS.gene062071:intron 20.0%
!!! TTTTACTTGCTTACATTTTT+TGG - chr2.3:45220010-45220029 MS.gene062071:intron 20.0%
! AATTAGGAGTGATTTGATAT+TGG - chr2.3:45219354-45219373 MS.gene062071:intron 25.0%
! ACAAGAAAAAGTAGGTAATT+AGG - chr2.3:45219338-45219357 MS.gene062071:intron 25.0%
! ACGAAAAACTATGAAAACAA+AGG + chr2.3:45218944-45218963 None:intergenic 25.0%
! ATCATGATTGGTATAATCAT+TGG + chr2.3:45219879-45219898 None:intergenic 25.0%
! CACATAAAGATAAATCGTTT+GGG + chr2.3:45219652-45219671 None:intergenic 25.0%
! CGAAAAACTATGAAAACAAA+GGG + chr2.3:45218943-45218962 None:intergenic 25.0%
! CGGATTCTGATTTAAATAAT+TGG + chr2.3:45219617-45219636 None:intergenic 25.0%
! GCTATAAAGTAAGTAAAACA+TGG - chr2.3:45219853-45219872 MS.gene062071:intron 25.0%
! TCACATAAAGATAAATCGTT+TGG + chr2.3:45219653-45219672 None:intergenic 25.0%
! TTGAGTATGTTACATAGTTA+TGG - chr2.3:45219274-45219293 MS.gene062071:intron 25.0%
!! AAATTCTCTTATTGGTGATT+TGG - chr2.3:45219729-45219748 MS.gene062071:intron 25.0%
!! TGTTTTACTTACTTTATAGC+CGG + chr2.3:45219853-45219872 None:intergenic 25.0%
!!! CTCATATTTATTTTGGTACT+TGG - chr2.3:45219819-45219838 MS.gene062071:intron 25.0%
!!! GTTTTGTTTTTTGAGTTTGA+AGG + chr2.3:45220122-45220141 None:intergenic 25.0%
!!! TTACTTGCTTACATTTTTTG+GGG - chr2.3:45220012-45220031 MS.gene062071:intron 25.0%
AATTAAGCACATATACTCCT+TGG - chr2.3:45219008-45219027 MS.gene062071:intron 30.0%
ATTTACCCCTTCATACATTT+GGG - chr2.3:45218842-45218861 MS.gene062071:CDS 30.0%
GACAATGCATAAAATATGCA+AGG - chr2.3:45219079-45219098 MS.gene062071:intron 30.0%
TGCATACTTCTTTAGCATAT+TGG + chr2.3:45220213-45220232 None:intergenic 30.0%
TTGAAGACAATGTCACTAAT+TGG - chr2.3:45219687-45219706 MS.gene062071:intron 30.0%
!!! TTTTGGGGTTAGAATTGTTT+GGG - chr2.3:45220027-45220046 MS.gene062071:intron 30.0%
!!! TTTTTGGGGTTAGAATTGTT+TGG - chr2.3:45220026-45220045 MS.gene062071:intron 30.0%
AAAGAGACATGATCATGTGT+TGG - chr2.3:45219130-45219149 MS.gene062071:intron 35.0%
ACAATGTCACTAATTGGCTA+TGG - chr2.3:45219693-45219712 MS.gene062071:intron 35.0%
ACATGATCATGTCTAATCCA+AGG + chr2.3:45219028-45219047 None:intergenic 35.0%
ACTCAAACCCAAATGTATGA+AGG + chr2.3:45218852-45218871 None:intergenic 35.0%
CTCAAACCCAAATGTATGAA+GGG + chr2.3:45218851-45218870 None:intergenic 35.0%
GATTTACCCCTTCATACATT+TGG - chr2.3:45218841-45218860 MS.gene062071:CDS 35.0%
GTGTCATCAAACAAAGTTTC+TGG - chr2.3:45218880-45218899 MS.gene062071:CDS 35.0%
TCAAACCCAAATGTATGAAG+GGG + chr2.3:45218850-45218869 None:intergenic 35.0%
TGCATAAAATATGCAAGGTC+CGG - chr2.3:45219084-45219103 MS.gene062071:intron 35.0%
! AAAACCAAAAGCTGCTGATT+TGG - chr2.3:45218819-45218838 MS.gene062071:CDS 35.0%
! AAATCCAAATCAGCAGCTTT+TGG + chr2.3:45218826-45218845 None:intergenic 35.0%
! ACTTTTCAGTGACTTGAAGA+TGG + chr2.3:45218906-45218925 None:intergenic 35.0%
! ATGATCATGTCTCTTTTTGG+TGG + chr2.3:45219126-45219145 None:intergenic 35.0%
! CACATGATCATGTCTCTTTT+TGG + chr2.3:45219129-45219148 None:intergenic 35.0%
! CATTTGAAATCAGCAATGCT+TGG - chr2.3:45219503-45219522 MS.gene062071:CDS 35.0%
!! TCTTTTGCATAATCTGGAAC+AGG + chr2.3:45220185-45220204 None:intergenic 35.0%
!!! ATGGTTTTGGCAACACTTAA+AGG + chr2.3:45219554-45219573 None:intergenic 35.0%
!!! TGGTTTTGGCAACACTTAAA+GGG + chr2.3:45219553-45219572 None:intergenic 35.0%
ACACGAACACCAACAAAACA+CGG + chr2.3:45219254-45219273 None:intergenic 40.0%
AGTTGAGCTCGAATCATGAT+TGG + chr2.3:45219891-45219910 None:intergenic 40.0%
CATAAAATATGCAAGGTCCG+GGG - chr2.3:45219086-45219105 MS.gene062071:intron 40.0%
CGTTTGGGAAAACTTGAGTA+CGG + chr2.3:45219637-45219656 None:intergenic 40.0%
GCATAAAATATGCAAGGTCC+GGG - chr2.3:45219085-45219104 MS.gene062071:intron 40.0%
TGAAAACAAAGGGTTTGACC+TGG + chr2.3:45218933-45218952 None:intergenic 40.0%
TGTGGGTATAAAAGAGTTGC+TGG - chr2.3:45219461-45219480 MS.gene062071:CDS 40.0%
! AAGTGTTGCCAAAACCATTC+AGG - chr2.3:45219556-45219575 MS.gene062071:CDS 40.0%
! AGCATTCACCTGAATGGTTT+TGG + chr2.3:45219567-45219586 None:intergenic 40.0%
! CTTTTAGTGTGCTTCATGGA+GGG - chr2.3:45219387-45219406 MS.gene062071:intron 40.0%
! GCAGCTTCTTTTGCATAATC+TGG + chr2.3:45220191-45220210 None:intergenic 40.0%
! GTTTTGCCGATGATGATTGA+TGG + chr2.3:45218804-45218823 None:intergenic 40.0%
! TGAGCTTAGTTCAGTTGGTA+TGG - chr2.3:45219057-45219076 MS.gene062071:intron 40.0%
! TGTCTATGTCCGTGTTTTGT+TGG - chr2.3:45219242-45219261 MS.gene062071:intron 40.0%
! TTTTAGTGTGCTTCATGGAG+GGG - chr2.3:45219388-45219407 MS.gene062071:intron 40.0%
!! ATCATGTCTCTTTTTGGTGG+TGG + chr2.3:45219123-45219142 None:intergenic 40.0%
!! GGTTTTGGCAACACTTAAAG+GGG + chr2.3:45219552-45219571 None:intergenic 40.0%
AGGTGTGGGATGTGAAAATC+TGG - chr2.3:45220087-45220106 MS.gene062071:intron 45.0%
CCTCCATGAAGCACACTAAA+AGG + chr2.3:45219389-45219408 None:intergenic 45.0%
GCTGAAGCTCTTGCAAGTAT+TGG - chr2.3:45219425-45219444 MS.gene062071:intron 45.0%
GGAGGACCATCAATCATCAT+CGG - chr2.3:45218795-45218814 MS.gene062071:CDS 45.0%
TGGGGTTAGAATTGTTTGGG+CGG - chr2.3:45220030-45220049 MS.gene062071:intron 45.0%
TTACACTTCGATCCTGTTGC+AGG - chr2.3:45220067-45220086 MS.gene062071:intron 45.0%
! ATTGGAGCACATGTTGCTTG+TGG - chr2.3:45219443-45219462 MS.gene062071:intron 45.0%
! CAGCCTTTTAGTGTGCTTCA+TGG - chr2.3:45219383-45219402 MS.gene062071:intron 45.0%
! CCTTTTAGTGTGCTTCATGG+AGG - chr2.3:45219386-45219405 MS.gene062071:intron 45.0%
! GATATGTGTCGGTATCACGT+CGG + chr2.3:45219175-45219194 None:intergenic 45.0%
! TTGGAGCACATGTTGCTTGT+GGG - chr2.3:45219444-45219463 MS.gene062071:intron 45.0%
!! AAGAGGAGCATTCACCTGAA+TGG + chr2.3:45219573-45219592 None:intergenic 45.0%
!! AGTCACTGAAAAGTGTTGCC+AGG - chr2.3:45218912-45218931 MS.gene062071:CDS 45.0%
CCAACTGAACTAAGCTCACG+AGG + chr2.3:45219055-45219074 None:intergenic 50.0%
CCTCGTGAGCTTAGTTCAGT+TGG - chr2.3:45219052-45219071 MS.gene062071:intron 50.0%
TTCGATCCTGTTGCAGGTGT+GGG - chr2.3:45220073-45220092 MS.gene062071:intron 50.0%
! TTGGTACTTGGCTCGATGTC+CGG - chr2.3:45219831-45219850 MS.gene062071:intron 50.0%
CTTCGATCCTGTTGCAGGTG+TGG - chr2.3:45220072-45220091 MS.gene062071:intron 55.0%
TCACATCCCACACCTGCAAC+AGG + chr2.3:45220082-45220101 None:intergenic 55.0%
!!! GTCTCTTTTTGGTGGTGGCC+AGG + chr2.3:45219118-45219137 None:intergenic 55.0%
CAAGGTCCGGGGTTCGAACC+TGG - chr2.3:45219097-45219116 MS.gene062071:intron 65.0%
TGGTGGCCAGGTTCGAACCC+CGG + chr2.3:45219106-45219125 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr2.3 gene 45218793 45220241 45218793 ID=MS.gene062071
chr2.3 mRNA 45218793 45220241 45218793 ID=MS.gene062071.t1;Parent=MS.gene062071
chr2.3 exon 45220101 45220241 45220101 ID=MS.gene062071.t1.exon1;Parent=MS.gene062071.t1
chr2.3 CDS 45220101 45220241 45220101 ID=cds.MS.gene062071.t1;Parent=MS.gene062071.t1
chr2.3 exon 45219457 45219648 45219457 ID=MS.gene062071.t1.exon2;Parent=MS.gene062071.t1
chr2.3 CDS 45219457 45219648 45219457 ID=cds.MS.gene062071.t1;Parent=MS.gene062071.t1
chr2.3 exon 45218793 45218945 45218793 ID=MS.gene062071.t1.exon3;Parent=MS.gene062071.t1
chr2.3 CDS 45218793 45218945 45218793 ID=cds.MS.gene062071.t1;Parent=MS.gene062071.t1
Gene Sequence

>MS.gene062071

ATGGAGGACCATCAATCATCATCGGCAAAACCAAAAGCTGCTGATTTGGATTTACCCCTTCATACATTTGGGTTTGAGTTTCAAGATGTGTCATCAAACAAAGTTTCTGGCCATCTTCAAGTCACTGAAAAGTGTTGCCAGGTCAAACCCTTTGTTTTCATAGTTTTTCGTCTCTTAATTCTTATAACAATTATTTGATACTATGATTCTTGAAAAATTAAGCACATATACTCCTTGGATTAGACATGATCATGTGTGTCCTCGTGAGCTTAGTTCAGTTGGTATGGACAATGCATAAAATATGCAAGGTCCGGGGTTCGAACCTGGCCACCACCAAAAAGAGACATGATCATGTGTTGGATATGTAACATGTCTGACACCGACGTGATACCGACACATATCATTATATTGAATTATGTTATTTTCTCAAATTATTATCAGCTTTGACGTGTCTATGTCCGTGTTTTGTTGGTGTTCGTGTTTGAGTATGTTACATAGTTATGGAATCATATTATTTTTTATATTAATTTCTCACATATATATGAACAAGAAAAAGTAGGTAATTAGGAGTGATTTGATATTGGATGTTGCAGCCTTTTAGTGTGCTTCATGGAGGGGTATCAGCAGTGATAGCTGAAGCTCTTGCAAGTATTGGAGCACATGTTGCTTGTGGGTATAAAAGAGTTGCTGGAATTCAACTTAGCATCAATCATTTGAAATCAGCAATGCTTGGTGACTTGATCTATGCTGAAGCAACCCCTTTAAGTGTTGCCAAAACCATTCAGGTGAATGCTCCTCTTTATATTTTTCTCAAAGTCTGACCAATTATTTAAATCAGAATCCGTACTCAAGTTTTCCCAAACGATTTATCTTTATGTGAAGCACACTATATAATTGAAGACAATGTCACTAATTGGCTATGGTTACCATTTATATAAATTCTCTTATTGGTGATTTGGCGCATAATAATAACAATAGAACATAAGACATACTCTAAGATGTTTTTGTAATTTTATTAGATATCATCTCATATTTATTTTGGTACTTGGCTCGATGTCCGGCTATAAAGTAAGTAAAACATGGCCAATGATTATACCAATCATGATTCGAGCTCAACTCTCTTAAACAATTCGTCTTTAACTGAAGTTCATTAATGACTTGAGTATAATCATTTGTTTGAATCATCTCATTGTTTACTCCAAATGTTGTGTTTAAATTTTTACTTGCTTACATTTTTTGGGGTTAGAATTGTTTGGGCGGATATGATCGTTATCTTACACTTCGATCCTGTTGCAGGTGTGGGATGTGAAAATCTGGAAGATCGATCCTTCAAACTCAAAAAACAAAACGTTGATAGCATATTCTAGAGTAACTCTTAAAAGCAACATGCCTGTTCCAGATTATGCAAAAGAAGCTGCCAATATGCTAAAGAAGTATGCAAAACTGTAA

Protein sequence

>MS.gene062071.t1

MEDHQSSSAKPKAADLDLPLHTFGFEFQDVSSNKVSGHLQVTEKCCQPFSVLHGGVSAVIAEALASIGAHVACGYKRVAGIQLSINHLKSAMLGDLIYAEATPLSVAKTIQVWDVKIWKIDPSNSKNKTLIAYSRVTLKSNMPVPDYAKEAANMLKKYAKL