Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene073954.t1 | XP_024630994.1 | 96.7 | 61 | 2 | 0 | 1 | 61 | 20 | 80 | 1.10E-22 | 115.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene073954.t1 | Q9T0H2 | 59.7 | 62 | 24 | 1 | 1 | 61 | 1 | 62 | 1.8e-10 | 65.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene073954.t1 | A0A1S2XYN4 | 73.8 | 61 | 15 | 1 | 1 | 61 | 1 | 60 | 7.9e-15 | 88.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049159 | MS.gene073954 | 0.823848 | 1.11E-53 | -1.69E-46 |
| MS.gene049255 | MS.gene073954 | 0.876643 | 1.19E-68 | -1.69E-46 |
| MS.gene049341 | MS.gene073954 | 0.822516 | 2.27E-53 | -1.69E-46 |
| MS.gene049382 | MS.gene073954 | 0.80724 | 5.57E-50 | -1.69E-46 |
| MS.gene049653 | MS.gene073954 | 0.817394 | 3.37E-52 | -1.69E-46 |
| MS.gene049756 | MS.gene073954 | 0.830257 | 3.25E-55 | -1.69E-46 |
| MS.gene049821 | MS.gene073954 | 0.806298 | 8.80E-50 | -1.69E-46 |
| MS.gene050020 | MS.gene073954 | 0.802301 | 5.99E-49 | -1.69E-46 |
| MS.gene050277 | MS.gene073954 | 0.845598 | 3.68E-59 | -1.69E-46 |
| MS.gene050303 | MS.gene073954 | 0.830779 | 2.42E-55 | -1.69E-46 |
| MS.gene050437 | MS.gene073954 | 0.813598 | 2.36E-51 | -1.69E-46 |
| MS.gene050623 | MS.gene073954 | -0.815347 | 9.69E-52 | -1.69E-46 |
| MS.gene050689 | MS.gene073954 | 0.813914 | 2.01E-51 | -1.69E-46 |
| MS.gene050721 | MS.gene073954 | 0.80201 | 6.87E-49 | -1.69E-46 |
| MS.gene050724 | MS.gene073954 | 0.814146 | 1.79E-51 | -1.69E-46 |
| MS.gene050780 | MS.gene073954 | 0.80044 | 1.44E-48 | -1.69E-46 |
| MS.gene050807 | MS.gene073954 | 0.818065 | 2.38E-52 | -1.69E-46 |
| MS.gene050815 | MS.gene073954 | 0.868686 | 5.43E-66 | -1.69E-46 |
| MS.gene051088 | MS.gene073954 | 0.804906 | 1.72E-49 | -1.69E-46 |
| MS.gene051241 | MS.gene073954 | 0.830286 | 3.20E-55 | -1.69E-46 |
| MS.gene051243 | MS.gene073954 | 0.871039 | 9.26E-67 | -1.69E-46 |
| MS.gene051245 | MS.gene073954 | 0.838057 | 3.60E-57 | -1.69E-46 |
| MS.gene051328 | MS.gene073954 | 0.860487 | 2.00E-63 | -1.69E-46 |
| MS.gene051349 | MS.gene073954 | 0.812069 | 5.10E-51 | -1.69E-46 |
| MS.gene05134 | MS.gene073954 | 0.839155 | 1.88E-57 | -1.69E-46 |
| MS.gene051451 | MS.gene073954 | 0.800876 | 1.17E-48 | -1.69E-46 |
| MS.gene051452 | MS.gene073954 | 0.838709 | 2.45E-57 | -1.69E-46 |
| MS.gene051535 | MS.gene073954 | 0.81393 | 1.99E-51 | -1.69E-46 |
| MS.gene051757 | MS.gene073954 | 0.804017 | 2.64E-49 | -1.69E-46 |
| MS.gene051789 | MS.gene073954 | 0.811036 | 8.56E-51 | -1.69E-46 |
| MS.gene05188 | MS.gene073954 | 0.831605 | 1.52E-55 | -1.69E-46 |
| MS.gene051975 | MS.gene073954 | 0.827022 | 1.97E-54 | -1.69E-46 |
| MS.gene052012 | MS.gene073954 | 0.843935 | 1.03E-58 | -1.69E-46 |
| MS.gene052286 | MS.gene073954 | 0.811511 | 6.75E-51 | -1.69E-46 |
| MS.gene052302 | MS.gene073954 | 0.800597 | 1.34E-48 | -1.69E-46 |
| MS.gene052376 | MS.gene073954 | 0.810849 | 9.40E-51 | -1.69E-46 |
| MS.gene052426 | MS.gene073954 | 0.814124 | 1.81E-51 | -1.69E-46 |
| MS.gene053006 | MS.gene073954 | 0.803222 | 3.86E-49 | -1.69E-46 |
| MS.gene053008 | MS.gene073954 | 0.869677 | 2.59E-66 | -1.69E-46 |
| MS.gene053058 | MS.gene073954 | 0.82545 | 4.66E-54 | -1.69E-46 |
| MS.gene053074 | MS.gene073954 | 0.825401 | 4.78E-54 | -1.69E-46 |
| MS.gene053080 | MS.gene073954 | 0.802175 | 6.36E-49 | -1.69E-46 |
| MS.gene053226 | MS.gene073954 | 0.817935 | 2.55E-52 | -1.69E-46 |
| MS.gene053590 | MS.gene073954 | 0.804171 | 2.46E-49 | -1.69E-46 |
| MS.gene053931 | MS.gene073954 | 0.815932 | 7.18E-52 | -1.69E-46 |
| MS.gene054472 | MS.gene073954 | 0.801807 | 7.56E-49 | -1.69E-46 |
| MS.gene054657 | MS.gene073954 | 0.850956 | 1.21E-60 | -1.69E-46 |
| MS.gene054658 | MS.gene073954 | 0.821145 | 4.72E-53 | -1.69E-46 |
| MS.gene05510 | MS.gene073954 | 0.825379 | 4.84E-54 | -1.69E-46 |
| MS.gene05517 | MS.gene073954 | 0.861876 | 7.54E-64 | -1.69E-46 |
| MS.gene055512 | MS.gene073954 | 0.821705 | 3.50E-53 | -1.69E-46 |
| MS.gene055623 | MS.gene073954 | 0.866786 | 2.21E-65 | -1.69E-46 |
| MS.gene055818 | MS.gene073954 | 0.811127 | 8.18E-51 | -1.69E-46 |
| MS.gene055841 | MS.gene073954 | 0.810771 | 9.77E-51 | -1.69E-46 |
| MS.gene056181 | MS.gene073954 | 0.803806 | 2.92E-49 | -1.69E-46 |
| MS.gene056506 | MS.gene073954 | 0.809115 | 2.22E-50 | -1.69E-46 |
| MS.gene056631 | MS.gene073954 | 0.861715 | 8.44E-64 | -1.69E-46 |
| MS.gene056760 | MS.gene073954 | 0.853202 | 2.79E-61 | -1.69E-46 |
| MS.gene056787 | MS.gene073954 | 0.802378 | 5.77E-49 | -1.69E-46 |
| MS.gene056986 | MS.gene073954 | 0.815806 | 7.66E-52 | -1.69E-46 |
| MS.gene057057 | MS.gene073954 | 0.801033 | 1.09E-48 | -1.69E-46 |
| MS.gene057060 | MS.gene073954 | 0.816447 | 5.50E-52 | -1.69E-46 |
| MS.gene057140 | MS.gene073954 | 0.873316 | 1.62E-67 | -1.69E-46 |
| MS.gene057208 | MS.gene073954 | 0.805256 | 1.46E-49 | -1.69E-46 |
| MS.gene057234 | MS.gene073954 | 0.802398 | 5.72E-49 | -1.69E-46 |
| MS.gene057392 | MS.gene073954 | 0.809842 | 1.55E-50 | -1.69E-46 |
| MS.gene057693 | MS.gene073954 | 0.85949 | 3.99E-63 | -1.69E-46 |
| MS.gene057705 | MS.gene073954 | 0.815028 | 1.14E-51 | -1.69E-46 |
| MS.gene05795 | MS.gene073954 | 0.810447 | 1.15E-50 | -1.69E-46 |
| MS.gene058084 | MS.gene073954 | 0.833037 | 6.71E-56 | -1.69E-46 |
| MS.gene058126 | MS.gene073954 | 0.81846 | 1.94E-52 | -1.69E-46 |
| MS.gene058146 | MS.gene073954 | 0.833813 | 4.30E-56 | -1.69E-46 |
| MS.gene058238 | MS.gene073954 | 0.836143 | 1.11E-56 | -1.69E-46 |
| MS.gene058548 | MS.gene073954 | 0.825975 | 3.50E-54 | -1.69E-46 |
| MS.gene058549 | MS.gene073954 | 0.814117 | 1.81E-51 | -1.69E-46 |
| MS.gene058595 | MS.gene073954 | 0.800051 | 1.73E-48 | -1.69E-46 |
| MS.gene058646 | MS.gene073954 | 0.804957 | 1.68E-49 | -1.69E-46 |
| MS.gene058906 | MS.gene073954 | 0.848903 | 4.56E-60 | -1.69E-46 |
| MS.gene058976 | MS.gene073954 | 0.809955 | 1.46E-50 | -1.69E-46 |
| MS.gene059006 | MS.gene073954 | 0.811003 | 8.70E-51 | -1.69E-46 |
| MS.gene059151 | MS.gene073954 | 0.802557 | 5.30E-49 | -1.69E-46 |
| MS.gene059317 | MS.gene073954 | 0.815666 | 8.22E-52 | -1.69E-46 |
| MS.gene059569 | MS.gene073954 | 0.835327 | 1.79E-56 | -1.69E-46 |
| MS.gene05978 | MS.gene073954 | 0.81334 | 2.69E-51 | -1.69E-46 |
| MS.gene05979 | MS.gene073954 | 0.836447 | 9.31E-57 | -1.69E-46 |
| MS.gene059830 | MS.gene073954 | 0.835958 | 1.24E-56 | -1.69E-46 |
| MS.gene059842 | MS.gene073954 | 0.829846 | 4.09E-55 | -1.69E-46 |
| MS.gene060296 | MS.gene073954 | 0.80179 | 7.63E-49 | -1.69E-46 |
| MS.gene060297 | MS.gene073954 | 0.806069 | 9.84E-50 | -1.69E-46 |
| MS.gene060310 | MS.gene073954 | 0.806667 | 7.36E-50 | -1.69E-46 |
| MS.gene060322 | MS.gene073954 | 0.835291 | 1.83E-56 | -1.69E-46 |
| MS.gene060429 | MS.gene073954 | 0.847747 | 9.51E-60 | -1.69E-46 |
| MS.gene060572 | MS.gene073954 | 0.806419 | 8.30E-50 | -1.69E-46 |
| MS.gene060586 | MS.gene073954 | 0.807205 | 5.66E-50 | -1.69E-46 |
| MS.gene060683 | MS.gene073954 | 0.80208 | 6.65E-49 | -1.69E-46 |
| MS.gene060685 | MS.gene073954 | 0.800875 | 1.17E-48 | -1.69E-46 |
| MS.gene060802 | MS.gene073954 | 0.820265 | 7.51E-53 | -1.69E-46 |
| MS.gene060803 | MS.gene073954 | 0.856723 | 2.65E-62 | -1.69E-46 |
| MS.gene060907 | MS.gene073954 | 0.815428 | 9.29E-52 | -1.69E-46 |
| MS.gene060998 | MS.gene073954 | 0.814571 | 1.44E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene073954.t1 | MTR_1g045890 | 94.286 | 35 | 2 | 0 | 1 | 35 | 20 | 54 | 6.88e-17 | 67.4 |
| MS.gene073954.t1 | MTR_1g045890 | 75.000 | 48 | 11 | 1 | 1 | 47 | 20 | 67 | 1.01e-16 | 67.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 13 sgRNAs with CRISPR-Local
Find 12 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGCGACTTTGCTATCATTGA+CGG | 0.418660 | 1.1:-28724877 | None:intergenic |
| ATGAGAACAATGGATTTGTT+CGG | 0.449405 | 1.1:+28724771 | MS.gene073954:CDS |
| ATCATTGACGGCAATACCTT+CGG | 0.457365 | 1.1:-28724865 | None:intergenic |
| GGATTTGTTCGGAGAGGAGA+TGG | 0.472087 | 1.1:+28724782 | MS.gene073954:CDS |
| CAGCATCATCGAAATCATCT+TGG | 0.487074 | 1.1:-28724923 | None:intergenic |
| TCAGATAACAACCATGGCTA+TGG | 0.492608 | 1.1:+28724809 | MS.gene073954:CDS |
| ATCATCGAAATCATCTTGGA+AGG | 0.593027 | 1.1:-28724919 | None:intergenic |
| AACAATGGATTTGTTCGGAG+AGG | 0.601463 | 1.1:+28724776 | MS.gene073954:CDS |
| AATTTCGATATCTTATCCGA+AGG | 0.608255 | 1.1:+28724849 | MS.gene073954:CDS |
| TTTGTTCGGAGAGGAGATGG+AGG | 0.616272 | 1.1:+28724785 | MS.gene073954:CDS |
| GTCATCAACATCCATAGCCA+TGG | 0.620045 | 1.1:-28724820 | None:intergenic |
| TAGTCGAAGAATGAGAACAA+TGG | 0.638863 | 1.1:+28724761 | None:intergenic |
| GGAGGATCAGATAACAACCA+TGG | 0.690661 | 1.1:+28724803 | MS.gene073954:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AATTTCGATATCTTATCCGA+AGG | + | chr1.1:28724849-28724868 | MS.gene073954:CDS | 30.0% | |
| ! | ATGAGAACAATGGATTTGTT+CGG | + | chr1.1:28724771-28724790 | MS.gene073954:CDS | 30.0% |
| ATCATCGAAATCATCTTGGA+AGG | - | chr1.1:28724922-28724941 | None:intergenic | 35.0% | |
| ATCATTGACGGCAATACCTT+CGG | - | chr1.1:28724868-28724887 | None:intergenic | 40.0% | |
| CAGCATCATCGAAATCATCT+TGG | - | chr1.1:28724926-28724945 | None:intergenic | 40.0% | |
| TCAGATAACAACCATGGCTA+TGG | + | chr1.1:28724809-28724828 | MS.gene073954:CDS | 40.0% | |
| ! | AACAATGGATTTGTTCGGAG+AGG | + | chr1.1:28724776-28724795 | MS.gene073954:CDS | 40.0% |
| ! | AGCGACTTTGCTATCATTGA+CGG | - | chr1.1:28724880-28724899 | None:intergenic | 40.0% |
| GGAGGATCAGATAACAACCA+TGG | + | chr1.1:28724803-28724822 | MS.gene073954:CDS | 45.0% | |
| GTCATCAACATCCATAGCCA+TGG | - | chr1.1:28724823-28724842 | None:intergenic | 45.0% | |
| TTTGTTCGGAGAGGAGATGG+AGG | + | chr1.1:28724785-28724804 | MS.gene073954:CDS | 50.0% | |
| ! | GGATTTGTTCGGAGAGGAGA+TGG | + | chr1.1:28724782-28724801 | MS.gene073954:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.1 | gene | 28724771 | 28724956 | 28724771 | ID=MS.gene073954 |
| chr1.1 | mRNA | 28724771 | 28724956 | 28724771 | ID=MS.gene073954.t1;Parent=MS.gene073954 |
| chr1.1 | exon | 28724771 | 28724956 | 28724771 | ID=MS.gene073954.t1.exon1;Parent=MS.gene073954.t1 |
| chr1.1 | CDS | 28724771 | 28724956 | 28724771 | ID=cds.MS.gene073954.t1;Parent=MS.gene073954.t1 |
| Gene Sequence |
| Protein sequence |
