AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene38712


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene38712.t1 MTR_3g101740 91.667 156 7 1 1 150 63 218 8.97e-97 278
MS.gene38712.t1 MTR_3g112410 37.168 113 63 2 33 145 13 117 4.92e-15 67.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene38712.t1 AT1G07700 76.515 132 30 1 14 145 74 204 5.99e-71 212
MS.gene38712.t1 AT1G07700 76.515 132 30 1 14 145 74 204 5.99e-71 212
MS.gene38712.t1 AT1G07700 76.515 132 30 1 14 145 74 204 5.99e-71 212
MS.gene38712.t1 AT1G07700 76.515 132 30 1 14 145 87 217 2.80e-70 211
MS.gene38712.t1 AT5G42980 33.628 113 67 2 33 145 12 116 2.26e-12 60.5
MS.gene38712.t1 AT3G51030 36.111 108 61 2 33 140 13 112 4.68e-12 59.7

Find 30 sgRNAs with CRISPR-Local

Find 48 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCTTTCTTTGTCTCTGGTT+GGG 0.298576 3.3:-84238638 None:intergenic
AACAATCCTTTCTTTGTCTC+TGG 0.330128 3.3:-84238643 None:intergenic
CTAGATAAGTCTAAAGGAAC+TGG 0.380813 3.3:+84237785 MS.gene38712:CDS
GCAAAGTTGTGCAAGAAATC+TGG 0.381106 3.3:+84237875 MS.gene38712:CDS
TTGCGGAACGACTTAGAATC+AGG 0.406477 3.3:+84238246 MS.gene38712:CDS
AATTCTCTCACACATTCAAC+AGG 0.416746 3.3:-84237734 None:intergenic
TTATGCTTTAGAAAGATAAC+AGG 0.421854 3.3:-84237911 None:intergenic
ATCCTTTCTTTGTCTCTGGT+TGG 0.431773 3.3:-84238639 None:intergenic
TGCAACTTCCACAAGAGGTT+CGG 0.443956 3.3:-84237832 None:intergenic
CTCTTCCACTTCTACAAAGA+TGG 0.449719 3.3:+84238597 MS.gene38712:CDS
GATTTCTTCCGAACCTCTTG+TGG 0.457067 3.3:+84237824 MS.gene38712:CDS
AAGCCTTGTTGACATTCAAC+GGG 0.478995 3.3:-84237683 None:intergenic
ACATCATCATCATCTTCATC+AGG 0.492703 3.3:-84237707 None:intergenic
CAAGCCTTGTTGACATTCAA+CGG 0.505128 3.3:-84237684 None:intergenic
AAGTTGCAAGTATATTGAGC+AGG 0.512637 3.3:+84237847 MS.gene38712:CDS
CTACAAAGATGGAAAACTGT+TGG 0.519244 3.3:+84238608 MS.gene38712:CDS
AAAATTCTAGATAAGTCTAA+AGG 0.519884 3.3:+84237779 MS.gene38712:CDS
TCTAAGTCGTTCCGCAACCT+CGG 0.520659 3.3:-84238240 None:intergenic
AACTGGATCTCTTGTTGTGG+TGG 0.529192 3.3:+84237802 MS.gene38712:CDS
TGATGAAGAATCCGAGGTTG+CGG 0.530168 3.3:+84238229 MS.gene38712:CDS
TCCCAACCAGAGACAAAGAA+AGG 0.565898 3.3:+84238637 MS.gene38712:CDS
ATACTTGCAACTTCCACAAG+AGG 0.567703 3.3:-84237837 None:intergenic
AGTTGCAAGTATATTGAGCA+GGG 0.585364 3.3:+84237848 MS.gene38712:CDS
CTTCAAGAGATGAGTACTTG+AGG 0.592510 3.3:-84238674 None:intergenic
AAGAGATGAGTACTTGAGGA+TGG 0.618825 3.3:-84238670 None:intergenic
TGCGGAACGACTTAGAATCA+GGG 0.632999 3.3:+84238247 MS.gene38712:CDS
TCTTTGTAGAAGTGGAAGAG+AGG 0.655662 3.3:-84238594 None:intergenic
AGGAACTGGATCTCTTGTTG+TGG 0.658617 3.3:+84237799 MS.gene38712:CDS
TATCCCGTTGAATGTCAACA+AGG 0.662409 3.3:+84237680 MS.gene38712:CDS
TGAGTATGATGAAGAATCCG+AGG 0.729308 3.3:+84238223 MS.gene38712:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTATTCAATAAGTTGATTT+TGG - chr3.3:84238550-84238569 None:intergenic 15.0%
!! AAAATTCTAGATAAGTCTAA+AGG + chr3.3:84237779-84237798 MS.gene38712:CDS 20.0%
!! ACTATTAGTTACAAATTAGT+TGG + chr3.3:84237985-84238004 MS.gene38712:intron 20.0%
!! TTAGATATTTATAGCATGTA+GGG - chr3.3:84238511-84238530 None:intergenic 20.0%
! AGAATACAAATGTGATACAT+TGG - chr3.3:84238082-84238101 None:intergenic 25.0%
! GTTAGATATTTATAGCATGT+AGG - chr3.3:84238512-84238531 None:intergenic 25.0%
! TAATAATCACAAACTAATGC+AGG + chr3.3:84238566-84238585 MS.gene38712:intron 25.0%
!! TATGGATTAAAAACTGTTAG+CGG + chr3.3:84238013-84238032 MS.gene38712:intron 25.0%
!!! TTATGCTTTAGAAAGATAAC+AGG - chr3.3:84237914-84237933 None:intergenic 25.0%
AAACATGAAGATGATACTGA+TGG - chr3.3:84238315-84238334 None:intergenic 30.0%
ATCAATTACCTGCAATAAGA+GGG - chr3.3:84238204-84238223 None:intergenic 30.0%
CTTAGTATGCATTCAATTGA+TGG + chr3.3:84238392-84238411 MS.gene38712:intron 30.0%
TTAACTGTTCAGTTTGTCTA+TGG + chr3.3:84237956-84237975 MS.gene38712:intron 30.0%
!! ACAAATTAGTTGGTGACTTA+TGG + chr3.3:84237995-84238014 MS.gene38712:intron 30.0%
!! GATGGATAATGTTGTTTCTT+AGG + chr3.3:84238410-84238429 MS.gene38712:intron 30.0%
AACAATCCTTTCTTTGTCTC+TGG - chr3.3:84238646-84238665 None:intergenic 35.0%
AAGTTGCAAGTATATTGAGC+AGG + chr3.3:84237847-84237866 MS.gene38712:CDS 35.0%
AATTCTCTCACACATTCAAC+AGG - chr3.3:84237737-84237756 None:intergenic 35.0%
ACATCATCATCATCTTCATC+AGG - chr3.3:84237710-84237729 None:intergenic 35.0%
AGTTGCAAGTATATTGAGCA+GGG + chr3.3:84237848-84237867 MS.gene38712:CDS 35.0%
CATCAATTACCTGCAATAAG+AGG - chr3.3:84238205-84238224 None:intergenic 35.0%
CTACAAAGATGGAAAACTGT+TGG + chr3.3:84238608-84238627 MS.gene38712:CDS 35.0%
CTAGATAAGTCTAAAGGAAC+TGG + chr3.3:84237785-84237804 MS.gene38712:CDS 35.0%
! GTTTTCCATCTTTGTAGAAG+TGG - chr3.3:84238605-84238624 None:intergenic 35.0%
AAGAGATGAGTACTTGAGGA+TGG - chr3.3:84238673-84238692 None:intergenic 40.0%
ATACTTGCAACTTCCACAAG+AGG - chr3.3:84237840-84237859 None:intergenic 40.0%
ATCCTTTCTTTGTCTCTGGT+TGG - chr3.3:84238642-84238661 None:intergenic 40.0%
CATGAAGATGATACTGATGG+CGG - chr3.3:84238312-84238331 None:intergenic 40.0%
CTCTTCCACTTCTACAAAGA+TGG + chr3.3:84238597-84238616 MS.gene38712:CDS 40.0%
CTGTTCAGTTTGTCTATGGT+TGG + chr3.3:84237960-84237979 MS.gene38712:intron 40.0%
CTTCAAGAGATGAGTACTTG+AGG - chr3.3:84238677-84238696 None:intergenic 40.0%
GCAAAGTTGTGCAAGAAATC+TGG + chr3.3:84237875-84237894 MS.gene38712:CDS 40.0%
TATCACTTCCCTCTTATTGC+AGG + chr3.3:84238193-84238212 MS.gene38712:intron 40.0%
TATCCCGTTGAATGTCAACA+AGG + chr3.3:84237680-84237699 MS.gene38712:CDS 40.0%
TCCTTTCTTTGTCTCTGGTT+GGG - chr3.3:84238641-84238660 None:intergenic 40.0%
TCTTTGTAGAAGTGGAAGAG+AGG - chr3.3:84238597-84238616 None:intergenic 40.0%
TGAGTATGATGAAGAATCCG+AGG + chr3.3:84238223-84238242 MS.gene38712:CDS 40.0%
!! AAGCCTTGTTGACATTCAAC+GGG - chr3.3:84237686-84237705 None:intergenic 40.0%
!! CAAGCCTTGTTGACATTCAA+CGG - chr3.3:84237687-84237706 None:intergenic 40.0%
AGGAACTGGATCTCTTGTTG+TGG + chr3.3:84237799-84237818 MS.gene38712:CDS 45.0%
GATTTCTTCCGAACCTCTTG+TGG + chr3.3:84237824-84237843 MS.gene38712:CDS 45.0%
TCCCAACCAGAGACAAAGAA+AGG + chr3.3:84238637-84238656 MS.gene38712:CDS 45.0%
TGATGAAGAATCCGAGGTTG+CGG + chr3.3:84238229-84238248 MS.gene38712:CDS 45.0%
TGCAACTTCCACAAGAGGTT+CGG - chr3.3:84237835-84237854 None:intergenic 45.0%
TGCGGAACGACTTAGAATCA+GGG + chr3.3:84238247-84238266 MS.gene38712:CDS 45.0%
TTGCGGAACGACTTAGAATC+AGG + chr3.3:84238246-84238265 MS.gene38712:CDS 45.0%
! AACTGGATCTCTTGTTGTGG+TGG + chr3.3:84237802-84237821 MS.gene38712:CDS 45.0%
TCTAAGTCGTTCCGCAACCT+CGG - chr3.3:84238243-84238262 None:intergenic 50.0%


Chromosome Type Strat End Strand Name
chr3.3 gene 84237665 84238716 84237665 ID=MS.gene38712
chr3.3 mRNA 84237665 84238716 84237665 ID=MS.gene38712.t1;Parent=MS.gene38712
chr3.3 exon 84237665 84237934 84237665 ID=MS.gene38712.t1.exon1;Parent=MS.gene38712.t1
chr3.3 CDS 84237665 84237934 84237665 ID=cds.MS.gene38712.t1;Parent=MS.gene38712.t1
chr3.3 exon 84238215 84238268 84238215 ID=MS.gene38712.t1.exon2;Parent=MS.gene38712.t1
chr3.3 CDS 84238215 84238268 84238215 ID=cds.MS.gene38712.t1;Parent=MS.gene38712.t1
chr3.3 exon 84238588 84238716 84238588 ID=MS.gene38712.t1.exon3;Parent=MS.gene38712.t1
chr3.3 CDS 84238588 84238716 84238588 ID=cds.MS.gene38712.t1;Parent=MS.gene38712.t1
Gene Sequence

>MS.gene38712

ATGAGGTACAATTTCTATCCCGTTGAATGTCAACAAGGCTTGCCTGATGAAGATGATGATGATGTCTGTCCTGTTGAATGTGTGAGAGAATTCAAGACTGATGAAGAGTTCTTGAAAATTCTAGATAAGTCTAAAGGAACTGGATCTCTTGTTGTGGTGGATTTCTTCCGAACCTCTTGTGGAAGTTGCAAGTATATTGAGCAGGGTTTTGCAAAGTTGTGCAAGAAATCTGGTAGCAATGATGTTCCTGTTATCTTTCTAAAGCATAATGTAAGTATTCTATATCTGTTGTTAACTGTTCAGTTTGTCTATGGTTGGTTACTATTAGTTACAAATTAGTTGGTGACTTATGGATTAAAAACTGTTAGCGGTGATTTGTGTTAGTTCAATGAGTTGTTTTTTATAAATAGAAAACCAATGTATCACATTTGTATTCTCATTATTTGCTTTATCATTGACTCTCAAACTAGAGATTTTATCTGATTAATAGTTAAACTGAGCATGTTAACTTTTATCTAGACTCTTAGTTATCACTTCCCTCTTATTGCAGGTAATTGATGAGTATGATGAAGAATCCGAGGTTGCGGAACGACTTAGAATCAGGGTATTTATTTCTCTTTTATTGATTTCAGACTAGCAAGTATCCGCCATCAGTATCATCTTCATGTTTCTTGTCTAATAATAACTTGTCTTTAAAATCTAGATGCTTCATTAGCATGATTACTCTCTTAGTATGCATTCAATTGATGGATAATGTTGTTTCTTAGGATGCATTCAATTGATGCGTAATGTTGATTACTCTCTCAAAACTATATTACTCTATTGATGATAATTTTATCTTCTCCCTACATGCTATAAATATCTAACTGTGCATCATTTTCACCAAAATCAACTTATTGAATAATAATCACAAACTAATGCAGGCAGTGCCTCTCTTCCACTTCTACAAAGATGGAAAACTGTTGGAAGCATTCCCAACCAGAGACAAAGAAAGGATTGTTGCTGCCATCCTCAAGTACTCATCTCTTGAAGCAGAAGATATTTTGAGCTAA

Protein sequence

>MS.gene38712.t1

MRYNFYPVECQQGLPDEDDDDVCPVECVREFKTDEEFLKILDKSKGTGSLVVVDFFRTSCGSCKYIEQGFAKLCKKSGSNDVPVIFLKHNVIDEYDEESEVAERLRIRAVPLFHFYKDGKLLEAFPTRDKERIVAAILKYSSLEAEDILS