Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene45806.t1 | RHN59274.1 | 96.8 | 440 | 14 | 0 | 1 | 440 | 8 | 447 | 6.10E-240 | 839.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene45806.t1 | O22145 | 74.2 | 438 | 107 | 3 | 4 | 440 | 44 | 476 | 5.1e-187 | 655.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene45806.t1 | G7JFU1 | 96.8 | 440 | 14 | 0 | 1 | 440 | 1 | 440 | 4.4e-240 | 839.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049171 | MS.gene45806 | 0.851573 | 8.13E-61 | -1.69E-46 |
| MS.gene049217 | MS.gene45806 | 0.823078 | 1.68E-53 | -1.69E-46 |
| MS.gene049222 | MS.gene45806 | 0.861422 | 1.04E-63 | -1.69E-46 |
| MS.gene049232 | MS.gene45806 | 0.862077 | 6.54E-64 | -1.69E-46 |
| MS.gene049285 | MS.gene45806 | 0.860903 | 1.49E-63 | -1.69E-46 |
| MS.gene049320 | MS.gene45806 | 0.838722 | 2.43E-57 | -1.69E-46 |
| MS.gene049609 | MS.gene45806 | 0.800939 | 1.14E-48 | -1.69E-46 |
| MS.gene049627 | MS.gene45806 | 0.846647 | 1.90E-59 | -1.69E-46 |
| MS.gene049633 | MS.gene45806 | 0.845612 | 3.64E-59 | -1.69E-46 |
| MS.gene049657 | MS.gene45806 | 0.803208 | 3.89E-49 | -1.69E-46 |
| MS.gene049865 | MS.gene45806 | 0.829171 | 5.98E-55 | -1.69E-46 |
| MS.gene05010 | MS.gene45806 | 0.847378 | 1.20E-59 | -1.69E-46 |
| MS.gene050163 | MS.gene45806 | 0.834286 | 3.27E-56 | -1.69E-46 |
| MS.gene050165 | MS.gene45806 | 0.84055 | 8.13E-58 | -1.69E-46 |
| MS.gene050170 | MS.gene45806 | 0.864552 | 1.12E-64 | -1.69E-46 |
| MS.gene050323 | MS.gene45806 | 0.860972 | 1.42E-63 | -1.69E-46 |
| MS.gene050333 | MS.gene45806 | 0.851713 | 7.42E-61 | -1.69E-46 |
| MS.gene050496 | MS.gene45806 | 0.859311 | 4.52E-63 | -1.69E-46 |
| MS.gene050504 | MS.gene45806 | 0.876856 | 1.00E-68 | -1.69E-46 |
| MS.gene050518 | MS.gene45806 | 0.867353 | 1.46E-65 | -1.69E-46 |
| MS.gene050577 | MS.gene45806 | 0.858123 | 1.02E-62 | -1.69E-46 |
| MS.gene050612 | MS.gene45806 | 0.848785 | 4.91E-60 | -1.69E-46 |
| MS.gene050675 | MS.gene45806 | 0.850847 | 1.30E-60 | -1.69E-46 |
| MS.gene050699 | MS.gene45806 | 0.850266 | 1.90E-60 | -1.69E-46 |
| MS.gene050747 | MS.gene45806 | 0.833229 | 6.01E-56 | -1.69E-46 |
| MS.gene050772 | MS.gene45806 | 0.825239 | 5.22E-54 | -1.69E-46 |
| MS.gene05080 | MS.gene45806 | 0.818535 | 1.86E-52 | -1.69E-46 |
| MS.gene050868 | MS.gene45806 | 0.858426 | 8.31E-63 | -1.69E-46 |
| MS.gene050871 | MS.gene45806 | 0.85612 | 3.99E-62 | -1.69E-46 |
| MS.gene050876 | MS.gene45806 | 0.853814 | 1.86E-61 | -1.69E-46 |
| MS.gene05087 | MS.gene45806 | 0.833001 | 6.85E-56 | -1.69E-46 |
| MS.gene050904 | MS.gene45806 | 0.883561 | 4.04E-71 | -1.69E-46 |
| MS.gene050950 | MS.gene45806 | 0.830213 | 3.33E-55 | -1.69E-46 |
| MS.gene050986 | MS.gene45806 | 0.864451 | 1.20E-64 | -1.69E-46 |
| MS.gene051268 | MS.gene45806 | 0.807379 | 5.20E-50 | -1.69E-46 |
| MS.gene051317 | MS.gene45806 | 0.873443 | 1.46E-67 | -1.69E-46 |
| MS.gene051551 | MS.gene45806 | 0.82983 | 4.13E-55 | -1.69E-46 |
| MS.gene052164 | MS.gene45806 | 0.83758 | 4.78E-57 | -1.69E-46 |
| MS.gene052482 | MS.gene45806 | 0.863014 | 3.37E-64 | -1.69E-46 |
| MS.gene052486 | MS.gene45806 | 0.876255 | 1.61E-68 | -1.69E-46 |
| MS.gene052503 | MS.gene45806 | 0.812525 | 4.06E-51 | -1.69E-46 |
| MS.gene052506 | MS.gene45806 | 0.807269 | 5.49E-50 | -1.69E-46 |
| MS.gene052822 | MS.gene45806 | 0.81471 | 1.34E-51 | -1.69E-46 |
| MS.gene052823 | MS.gene45806 | 0.814286 | 1.66E-51 | -1.69E-46 |
| MS.gene052825 | MS.gene45806 | 0.809554 | 1.79E-50 | -1.69E-46 |
| MS.gene052966 | MS.gene45806 | 0.854246 | 1.40E-61 | -1.69E-46 |
| MS.gene053203 | MS.gene45806 | 0.865548 | 5.45E-65 | -1.69E-46 |
| MS.gene053213 | MS.gene45806 | 0.832453 | 9.37E-56 | -1.69E-46 |
| MS.gene053286 | MS.gene45806 | 0.835966 | 1.23E-56 | -1.69E-46 |
| MS.gene053420 | MS.gene45806 | -0.802887 | 4.53E-49 | -1.69E-46 |
| MS.gene053479 | MS.gene45806 | 0.81093 | 9.02E-51 | -1.69E-46 |
| MS.gene053480 | MS.gene45806 | 0.822471 | 2.33E-53 | -1.69E-46 |
| MS.gene053574 | MS.gene45806 | 0.825435 | 4.70E-54 | -1.69E-46 |
| MS.gene053626 | MS.gene45806 | 0.826983 | 2.01E-54 | -1.69E-46 |
| MS.gene053633 | MS.gene45806 | 0.811008 | 8.68E-51 | -1.69E-46 |
| MS.gene053824 | MS.gene45806 | 0.849543 | 3.02E-60 | -1.69E-46 |
| MS.gene054408 | MS.gene45806 | 0.809284 | 2.04E-50 | -1.69E-46 |
| MS.gene054595 | MS.gene45806 | 0.838429 | 2.89E-57 | -1.69E-46 |
| MS.gene054600 | MS.gene45806 | 0.816959 | 4.23E-52 | -1.69E-46 |
| MS.gene054638 | MS.gene45806 | 0.818365 | 2.04E-52 | -1.69E-46 |
| MS.gene054642 | MS.gene45806 | 0.820716 | 5.92E-53 | -1.69E-46 |
| MS.gene05470 | MS.gene45806 | 0.854222 | 1.42E-61 | -1.69E-46 |
| MS.gene054954 | MS.gene45806 | 0.873068 | 1.96E-67 | -1.69E-46 |
| MS.gene055362 | MS.gene45806 | 0.849298 | 3.54E-60 | -1.69E-46 |
| MS.gene055502 | MS.gene45806 | 0.854449 | 1.22E-61 | -1.69E-46 |
| MS.gene055577 | MS.gene45806 | 0.861912 | 7.35E-64 | -1.69E-46 |
| MS.gene055748 | MS.gene45806 | 0.856214 | 3.74E-62 | -1.69E-46 |
| MS.gene055750 | MS.gene45806 | 0.866472 | 2.78E-65 | -1.69E-46 |
| MS.gene055751 | MS.gene45806 | 0.866117 | 3.60E-65 | -1.69E-46 |
| MS.gene055776 | MS.gene45806 | 0.807763 | 4.31E-50 | -1.69E-46 |
| MS.gene05589 | MS.gene45806 | 0.851542 | 8.29E-61 | -1.69E-46 |
| MS.gene055941 | MS.gene45806 | 0.802608 | 5.17E-49 | -1.69E-46 |
| MS.gene056040 | MS.gene45806 | 0.803442 | 3.48E-49 | -1.69E-46 |
| MS.gene056409 | MS.gene45806 | 0.855783 | 5.00E-62 | -1.69E-46 |
| MS.gene056449 | MS.gene45806 | 0.807048 | 6.11E-50 | -1.69E-46 |
| MS.gene056450 | MS.gene45806 | 0.882424 | 1.05E-70 | -1.69E-46 |
| MS.gene056504 | MS.gene45806 | 0.865485 | 5.71E-65 | -1.69E-46 |
| MS.gene056510 | MS.gene45806 | 0.869037 | 4.18E-66 | -1.69E-46 |
| MS.gene056610 | MS.gene45806 | 0.842727 | 2.17E-58 | -1.69E-46 |
| MS.gene056657 | MS.gene45806 | 0.839536 | 1.49E-57 | -1.69E-46 |
| MS.gene056692 | MS.gene45806 | 0.871368 | 7.21E-67 | -1.69E-46 |
| MS.gene056878 | MS.gene45806 | 0.812911 | 3.34E-51 | -1.69E-46 |
| MS.gene056898 | MS.gene45806 | 0.800826 | 1.20E-48 | -1.69E-46 |
| MS.gene057658 | MS.gene45806 | 0.813277 | 2.78E-51 | -1.69E-46 |
| MS.gene057831 | MS.gene45806 | 0.856734 | 2.63E-62 | -1.69E-46 |
| MS.gene057872 | MS.gene45806 | 0.825951 | 3.54E-54 | -1.69E-46 |
| MS.gene05787 | MS.gene45806 | 0.818782 | 1.64E-52 | -1.69E-46 |
| MS.gene058157 | MS.gene45806 | 0.822658 | 2.11E-53 | -1.69E-46 |
| MS.gene058334 | MS.gene45806 | -0.817642 | 2.97E-52 | -1.69E-46 |
| MS.gene05837 | MS.gene45806 | 0.859714 | 3.42E-63 | -1.69E-46 |
| MS.gene058419 | MS.gene45806 | 0.833935 | 4.01E-56 | -1.69E-46 |
| MS.gene058544 | MS.gene45806 | 0.815143 | 1.08E-51 | -1.69E-46 |
| MS.gene058719 | MS.gene45806 | 0.826713 | 2.33E-54 | -1.69E-46 |
| MS.gene058773 | MS.gene45806 | 0.857169 | 1.96E-62 | -1.69E-46 |
| MS.gene058838 | MS.gene45806 | 0.815488 | 9.01E-52 | -1.69E-46 |
| MS.gene059022 | MS.gene45806 | 0.857305 | 1.79E-62 | -1.69E-46 |
| MS.gene059023 | MS.gene45806 | 0.871428 | 6.89E-67 | -1.69E-46 |
| MS.gene05912 | MS.gene45806 | 0.802833 | 4.65E-49 | -1.69E-46 |
| MS.gene059181 | MS.gene45806 | 0.812545 | 4.02E-51 | -1.69E-46 |
| MS.gene059677 | MS.gene45806 | 0.855922 | 4.56E-62 | -1.69E-46 |
| MS.gene059718 | MS.gene45806 | 0.851526 | 8.38E-61 | -1.69E-46 |
| MS.gene059756 | MS.gene45806 | 0.868283 | 7.32E-66 | -1.69E-46 |
| MS.gene059801 | MS.gene45806 | 0.834789 | 2.45E-56 | -1.69E-46 |
| MS.gene059837 | MS.gene45806 | 0.846394 | 2.23E-59 | -1.69E-46 |
| MS.gene059865 | MS.gene45806 | 0.851225 | 1.02E-60 | -1.69E-46 |
| MS.gene06000 | MS.gene45806 | 0.845041 | 5.20E-59 | -1.69E-46 |
| MS.gene060595 | MS.gene45806 | -0.832546 | 8.89E-56 | -1.69E-46 |
| MS.gene060703 | MS.gene45806 | 0.834538 | 2.83E-56 | -1.69E-46 |
| MS.gene060762 | MS.gene45806 | 0.872766 | 2.47E-67 | -1.69E-46 |
| MS.gene060778 | MS.gene45806 | 0.840474 | 8.51E-58 | -1.69E-46 |
| MS.gene06077 | MS.gene45806 | 0.891728 | 3.05E-74 | -1.69E-46 |
| MS.gene06092 | MS.gene45806 | 0.806264 | 8.95E-50 | -1.69E-46 |
| MS.gene060982 | MS.gene45806 | 0.867688 | 1.14E-65 | -1.69E-46 |
| MS.gene061150 | MS.gene45806 | 0.837077 | 6.43E-57 | -1.69E-46 |
| MS.gene061171 | MS.gene45806 | 0.800981 | 1.12E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene45806.t1 | MTR_4g024920 | 96.818 | 440 | 14 | 0 | 1 | 440 | 1 | 440 | 0.0 | 876 |
| MS.gene45806.t1 | MTR_4g024920 | 96.364 | 440 | 15 | 1 | 1 | 440 | 24 | 462 | 0.0 | 868 |
| MS.gene45806.t1 | MTR_1g090817 | 29.464 | 336 | 210 | 10 | 49 | 383 | 1 | 310 | 1.70e-28 | 115 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene45806.t1 | AT2G45270 | 70.450 | 467 | 108 | 4 | 4 | 440 | 10 | 476 | 0.0 | 684 |
| MS.gene45806.t1 | AT4G22720 | 27.222 | 360 | 232 | 10 | 49 | 405 | 5 | 337 | 1.87e-26 | 109 |
| MS.gene45806.t1 | AT4G22720 | 27.222 | 360 | 232 | 10 | 49 | 405 | 5 | 337 | 1.87e-26 | 109 |
Find 111 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGCCGAAGAAATAGAAATTT+TGG | 0.151870 | 4.4:-76676493 | None:intergenic |
| CGCTGCGGGTAGAGTGTTTA+TGG | 0.165878 | 4.4:-76669315 | None:intergenic |
| TTCATGGCTTTACTTATTTC+AGG | 0.170241 | 4.4:+76673889 | MS.gene45806:CDS |
| CGATGGAGATATTGTCGTTT+TGG | 0.239610 | 4.4:+76669369 | MS.gene45806:CDS |
| CTCATTCTTGCACGTGATCT+TGG | 0.264225 | 4.4:+76675714 | MS.gene45806:CDS |
| ACTGCAGTTGCTGTTACTAT+TGG | 0.293726 | 4.4:+76672331 | MS.gene45806:CDS |
| ACAATATCTCCATCGCGTTT+TGG | 0.306487 | 4.4:-76669361 | None:intergenic |
| CAGAAGGCTCGGAGAATTGC+AGG | 0.314185 | 4.4:+76673592 | MS.gene45806:CDS |
| AGATTTGCAGTTCCCGTTCA+TGG | 0.329140 | 4.4:+76673873 | MS.gene45806:CDS |
| ACTCAATTGCCAGTCTTACT+TGG | 0.331056 | 4.4:-76676098 | None:intergenic |
| GCAGACCTGCTTGTGAAGTA+TGG | 0.338654 | 4.4:+76671582 | MS.gene45806:CDS |
| TGCCAAAATTTCTATTTCTT+CGG | 0.359407 | 4.4:+76676491 | MS.gene45806:CDS |
| AGAAGAACCAGAAGACTTTC+TGG | 0.361040 | 4.4:+76677243 | MS.gene45806:CDS |
| CAATCCTTATGCTGCCTCAT+TGG | 0.365186 | 4.4:-76676048 | None:intergenic |
| AGATGTGAAGAGTGAAGGTT+TGG | 0.374267 | 4.4:-76669272 | None:intergenic |
| ATGAATTCGTGCCGTTCTTA+AGG | 0.381153 | 4.4:-76677626 | None:intergenic |
| AATGGCTTGGACTTGATATA+AGG | 0.384502 | 4.4:+76675802 | MS.gene45806:CDS |
| TGTCTGTTGCAGGTTGTCTC+TGG | 0.399323 | 4.4:+76676873 | MS.gene45806:intron |
| AGTTGCTGTTACTATTGGTC+CGG | 0.399928 | 4.4:+76672336 | MS.gene45806:CDS |
| TGTACGCCCAAGGTGGCCGC+TGG | 0.403286 | 4.4:+76677567 | MS.gene45806:CDS |
| GAGTCAGGTTATATCTTCTC+AGG | 0.414648 | 4.4:+76669544 | MS.gene45806:CDS |
| GGCTTTAATTTACCAATTGT+TGG | 0.414914 | 4.4:+76673616 | MS.gene45806:CDS |
| GGTTCTTCTGCAGGAGGTGG+AGG | 0.418700 | 4.4:-76677229 | None:intergenic |
| TCATTCTTGCACGTGATCTT+GGG | 0.424423 | 4.4:+76675715 | MS.gene45806:CDS |
| AAACATACCAGAAAGTCTTC+TGG | 0.427747 | 4.4:-76677250 | None:intergenic |
| TTGCGTCAAATCAATATGTT+CGG | 0.427836 | 4.4:+76676901 | MS.gene45806:CDS |
| TCTGGTTCTTCTGCAGGAGG+TGG | 0.428517 | 4.4:-76677232 | None:intergenic |
| GAATAAATCATTACCATTGT+CGG | 0.429178 | 4.4:-76676991 | None:intergenic |
| AAATTTACGCGGGAAATTGT+TGG | 0.431992 | 4.4:-76669244 | None:intergenic |
| AAGTCTTCTGGTTCTTCTGC+AGG | 0.443693 | 4.4:-76677238 | None:intergenic |
| AACTAGTATGCCCTCCTCCT+CGG | 0.447114 | 4.4:+76676961 | MS.gene45806:CDS |
| CTCAATTGCCAGTCTTACTT+GGG | 0.447580 | 4.4:-76676097 | None:intergenic |
| CAGTCATATAAGCTTTATCA+AGG | 0.452366 | 4.4:-76672297 | None:intergenic |
| ACTACCAGATGTCTTATAGA+AGG | 0.455291 | 4.4:-76676769 | None:intergenic |
| GTTTATGGGGAAGATGAATT+CGG | 0.460133 | 4.4:-76669300 | None:intergenic |
| CTTATGACAAGTCAGCAAAA+TGG | 0.461556 | 4.4:+76675784 | MS.gene45806:CDS |
| AAGTGGTGGTCCTGCTATAG+AGG | 0.468477 | 4.4:+76675827 | MS.gene45806:CDS |
| CTTGGGCAATACATACAACT+TGG | 0.471005 | 4.4:+76675732 | MS.gene45806:CDS |
| GCTGCGGGTAGAGTGTTTAT+GGG | 0.473782 | 4.4:-76669314 | None:intergenic |
| GGAGCCTTCTATAAGACATC+TGG | 0.477018 | 4.4:+76676765 | MS.gene45806:CDS |
| GATTGCAACTTCTCTTATGC+TGG | 0.480476 | 4.4:+76676066 | MS.gene45806:CDS |
| TGCGTCAAATCAATATGTTC+GGG | 0.489887 | 4.4:+76676902 | MS.gene45806:CDS |
| ATGTGATGGACGCCAACAAT+TGG | 0.491621 | 4.4:-76673628 | None:intergenic |
| AAATAAGTAAAGCCATGAAC+GGG | 0.493745 | 4.4:-76673885 | None:intergenic |
| GTTGCTGTTACTATTGGTCC+GGG | 0.495817 | 4.4:+76672337 | MS.gene45806:CDS |
| GTACGCCCAAGGTGGCCGCT+GGG | 0.501523 | 4.4:+76677568 | MS.gene45806:CDS |
| GGTTCCAATGAGGCAGCATA+AGG | 0.503532 | 4.4:+76676044 | MS.gene45806:intron |
| GACAAGTCAGCAAAATGGCT+TGG | 0.508751 | 4.4:+76675789 | MS.gene45806:CDS |
| TTGGTTTAGGTGAGAAGTGA+CGG | 0.511315 | 4.4:+76669512 | MS.gene45806:intron |
| TGCTATAGAGGAGCTTGCTA+AGG | 0.520558 | 4.4:+76675839 | MS.gene45806:CDS |
| TCGGTACAAAGCCGAGGAGG+AGG | 0.523415 | 4.4:-76676972 | None:intergenic |
| GAATAGGTGTAATGGTTGCT+TGG | 0.523654 | 4.4:+76677170 | MS.gene45806:intron |
| GGTTTAAGTCTCTGCCTTCG+TGG | 0.527530 | 4.4:+76672358 | MS.gene45806:CDS |
| CTGTTGCAGGTTGTCTCTGG+TGG | 0.533183 | 4.4:+76676876 | MS.gene45806:intron |
| GAGGAGGGCATACTAGTTGA+AGG | 0.534013 | 4.4:-76676956 | None:intergenic |
| GATACGGTTGTGAAGAAGAA+TGG | 0.534130 | 4.4:+76676933 | MS.gene45806:CDS |
| CATGGAGGCTCATGCTCTAG+TGG | 0.537949 | 4.4:+76673648 | MS.gene45806:CDS |
| TGTTCGGGCTCAGCTTGATA+CGG | 0.538444 | 4.4:+76676917 | MS.gene45806:CDS |
| GGTGTAATGGTTGCTTGGAC+TGG | 0.539870 | 4.4:+76677175 | MS.gene45806:intron |
| ACTACAATAGATGATGCTAT+TGG | 0.540839 | 4.4:+76675756 | MS.gene45806:CDS |
| TACGCCCAAGGTGGCCGCTG+GGG | 0.542060 | 4.4:+76677569 | MS.gene45806:CDS |
| AATTCAGTGGGCATTGAAGA+TGG | 0.542394 | 4.4:+76676744 | MS.gene45806:CDS |
| TTAGCAAGCTCCTCTATAGC+AGG | 0.544377 | 4.4:-76675837 | None:intergenic |
| AAAATAAAAGCTTACGATAG+CGG | 0.546361 | 4.4:-76669422 | None:intergenic |
| TCACTCGCAAGTGATAGATC+AGG | 0.549311 | 4.4:+76671635 | MS.gene45806:CDS |
| TGAATTAGAGATGTAAGAGA+AGG | 0.551377 | 4.4:-76677649 | None:intergenic |
| AATTGCAGTATTACATCTTG+AGG | 0.551614 | 4.4:+76676705 | MS.gene45806:CDS |
| ACGCCCAAGGTGGCCGCTGG+GGG | 0.559762 | 4.4:+76677570 | MS.gene45806:CDS |
| AATTGTTGGCGTCCATCACA+TGG | 0.562392 | 4.4:+76673630 | MS.gene45806:CDS |
| TCTTCTGGTTCTTCTGCAGG+AGG | 0.563864 | 4.4:-76677235 | None:intergenic |
| GAGCATGAGCCTCCATGTGA+TGG | 0.565179 | 4.4:-76673642 | None:intergenic |
| TATAGAGGAGCTTGCTAAGG+AGG | 0.565375 | 4.4:+76675842 | MS.gene45806:CDS |
| CGCCTCCATACTTCACAAGC+AGG | 0.575654 | 4.4:-76671587 | None:intergenic |
| CAGTATTACATCTTGAGGAA+AGG | 0.576903 | 4.4:+76676710 | MS.gene45806:CDS |
| CGGAGAGATGTGAAGAGTGA+AGG | 0.577110 | 4.4:-76669277 | None:intergenic |
| GATGTGAAGAGTGAAGGTTT+GGG | 0.579788 | 4.4:-76669271 | None:intergenic |
| GAAATAAGTAAAGCCATGAA+CGG | 0.580635 | 4.4:-76673886 | None:intergenic |
| CCTCGGCTTTGTACCGACAA+TGG | 0.582726 | 4.4:+76676978 | MS.gene45806:CDS |
| TTCAGTATGATGTACGCCCA+AGG | 0.592571 | 4.4:+76677557 | MS.gene45806:intron |
| GGGGAAGCAAGAGACGCTGC+GGG | 0.594025 | 4.4:-76669329 | None:intergenic |
| CGGTACAAAGCCGAGGAGGA+GGG | 0.596485 | 4.4:-76676971 | None:intergenic |
| AGAAGGCTCGGAGAATTGCA+GGG | 0.598558 | 4.4:+76673593 | MS.gene45806:CDS |
| GGACTTGATATAAGGAGAAG+TGG | 0.600241 | 4.4:+76675810 | MS.gene45806:CDS |
| TAAAGCTTATATGACTGAGA+AGG | 0.603601 | 4.4:+76672303 | MS.gene45806:CDS |
| ATAACTAACCTGAAAAGAAG+CGG | 0.603786 | 4.4:-76676550 | None:intergenic |
| AGGCTCATGCTCTAGTGGCT+AGG | 0.604785 | 4.4:+76673653 | MS.gene45806:CDS |
| ATAGAGGAGCTTGCTAAGGA+GGG | 0.607465 | 4.4:+76675843 | MS.gene45806:CDS |
| TTTCAGTCGCTCCTTAAGAA+CGG | 0.607827 | 4.4:+76677615 | MS.gene45806:CDS |
| GGTATTGAGCACTTTCGCGT+AGG | 0.608335 | 4.4:+76677196 | MS.gene45806:CDS |
| AACCGAACTCCAAAACGCGA+TGG | 0.612279 | 4.4:+76669352 | MS.gene45806:CDS |
| GAAGGCTCGGAGAATTGCAG+GGG | 0.619023 | 4.4:+76673594 | MS.gene45806:CDS |
| GGTGTGAACGAGCAATTCAG+TGG | 0.634788 | 4.4:+76676731 | MS.gene45806:CDS |
| AGTCATATAAGCTTTATCAA+GGG | 0.646467 | 4.4:-76672296 | None:intergenic |
| GTTTGCTGTAGGTTCCAATG+AGG | 0.646943 | 4.4:+76676034 | MS.gene45806:intron |
| AATAGATGATGCTATTGGTG+AGG | 0.648851 | 4.4:+76675761 | MS.gene45806:CDS |
| GTGTGAACGAGCAATTCAGT+GGG | 0.650776 | 4.4:+76676732 | MS.gene45806:CDS |
| CTGCGGGTAGAGTGTTTATG+GGG | 0.652166 | 4.4:-76669313 | None:intergenic |
| GACCTGCTTGTGAAGTATGG+AGG | 0.654035 | 4.4:+76671585 | MS.gene45806:CDS |
| TATGGGGAAGATGAATTCGG+CGG | 0.655822 | 4.4:-76669297 | None:intergenic |
| TTTGGGAAAGAAATTTACGC+GGG | 0.658999 | 4.4:-76669254 | None:intergenic |
| TGGGGAAGCAAGAGACGCTG+CGG | 0.660145 | 4.4:-76669330 | None:intergenic |
| CTTGATATAAGGAGAAGTGG+TGG | 0.665095 | 4.4:+76675813 | MS.gene45806:CDS |
| CGAAGGCAGAGACTTAAACC+CGG | 0.668882 | 4.4:-76672355 | None:intergenic |
| TTGTCGGTACAAAGCCGAGG+AGG | 0.690039 | 4.4:-76676975 | None:intergenic |
| CCATTGTCGGTACAAAGCCG+AGG | 0.695752 | 4.4:-76676978 | None:intergenic |
| ATAAAAGCTTACGATAGCGG+CGG | 0.697433 | 4.4:-76669419 | None:intergenic |
| AAGGCTCGGAGAATTGCAGG+GGG | 0.698297 | 4.4:+76673595 | MS.gene45806:CDS |
| GTTTGGGAAAGAAATTTACG+CGG | 0.712488 | 4.4:-76669255 | None:intergenic |
| AGTATGATGTACGCCCAAGG+TGG | 0.716562 | 4.4:+76677560 | MS.gene45806:intron |
| AAGTTCGAGTATTACCACGA+AGG | 0.736459 | 4.4:-76672372 | None:intergenic |
| TGTTGGCGTCCATCACATGG+AGG | 0.747158 | 4.4:+76673633 | MS.gene45806:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 76669235 | 76677690 | 76669235 | ID=MS.gene45806 |
| chr4.4 | mRNA | 76669235 | 76677690 | 76669235 | ID=MS.gene45806.t1;Parent=MS.gene45806 |
| chr4.4 | exon | 76669235 | 76669429 | 76669235 | ID=MS.gene45806.t1.exon1;Parent=MS.gene45806.t1 |
| chr4.4 | CDS | 76669235 | 76669429 | 76669235 | ID=cds.MS.gene45806.t1;Parent=MS.gene45806.t1 |
| chr4.4 | exon | 76669521 | 76669565 | 76669521 | ID=MS.gene45806.t1.exon2;Parent=MS.gene45806.t1 |
| chr4.4 | CDS | 76669521 | 76669565 | 76669521 | ID=cds.MS.gene45806.t1;Parent=MS.gene45806.t1 |
| chr4.4 | exon | 76671582 | 76671656 | 76671582 | ID=MS.gene45806.t1.exon3;Parent=MS.gene45806.t1 |
| chr4.4 | CDS | 76671582 | 76671656 | 76671582 | ID=cds.MS.gene45806.t1;Parent=MS.gene45806.t1 |
| chr4.4 | exon | 76672283 | 76672379 | 76672283 | ID=MS.gene45806.t1.exon4;Parent=MS.gene45806.t1 |
| chr4.4 | CDS | 76672283 | 76672379 | 76672283 | ID=cds.MS.gene45806.t1;Parent=MS.gene45806.t1 |
| chr4.4 | exon | 76673584 | 76673674 | 76673584 | ID=MS.gene45806.t1.exon5;Parent=MS.gene45806.t1 |
| chr4.4 | CDS | 76673584 | 76673674 | 76673584 | ID=cds.MS.gene45806.t1;Parent=MS.gene45806.t1 |
| chr4.4 | exon | 76673861 | 76673910 | 76673861 | ID=MS.gene45806.t1.exon6;Parent=MS.gene45806.t1 |
| chr4.4 | CDS | 76673861 | 76673910 | 76673861 | ID=cds.MS.gene45806.t1;Parent=MS.gene45806.t1 |
| chr4.4 | exon | 76675700 | 76675890 | 76675700 | ID=MS.gene45806.t1.exon7;Parent=MS.gene45806.t1 |
| chr4.4 | CDS | 76675700 | 76675890 | 76675700 | ID=cds.MS.gene45806.t1;Parent=MS.gene45806.t1 |
| chr4.4 | exon | 76676045 | 76676130 | 76676045 | ID=MS.gene45806.t1.exon8;Parent=MS.gene45806.t1 |
| chr4.4 | CDS | 76676045 | 76676130 | 76676045 | ID=cds.MS.gene45806.t1;Parent=MS.gene45806.t1 |
| chr4.4 | exon | 76676488 | 76676563 | 76676488 | ID=MS.gene45806.t1.exon9;Parent=MS.gene45806.t1 |
| chr4.4 | CDS | 76676488 | 76676563 | 76676488 | ID=cds.MS.gene45806.t1;Parent=MS.gene45806.t1 |
| chr4.4 | exon | 76676703 | 76676786 | 76676703 | ID=MS.gene45806.t1.exon10;Parent=MS.gene45806.t1 |
| chr4.4 | CDS | 76676703 | 76676786 | 76676703 | ID=cds.MS.gene45806.t1;Parent=MS.gene45806.t1 |
| chr4.4 | exon | 76676885 | 76676999 | 76676885 | ID=MS.gene45806.t1.exon11;Parent=MS.gene45806.t1 |
| chr4.4 | CDS | 76676885 | 76676999 | 76676885 | ID=cds.MS.gene45806.t1;Parent=MS.gene45806.t1 |
| chr4.4 | exon | 76677176 | 76677264 | 76677176 | ID=MS.gene45806.t1.exon12;Parent=MS.gene45806.t1 |
| chr4.4 | CDS | 76677176 | 76677264 | 76677176 | ID=cds.MS.gene45806.t1;Parent=MS.gene45806.t1 |
| chr4.4 | exon | 76677562 | 76677690 | 76677562 | ID=MS.gene45806.t1.exon13;Parent=MS.gene45806.t1 |
| chr4.4 | CDS | 76677562 | 76677690 | 76677562 | ID=cds.MS.gene45806.t1;Parent=MS.gene45806.t1 |
| Gene Sequence |
| Protein sequence |
