AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene90793


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene90793.t1 MTR_1g116170 89.143 175 15 2 1 175 1 171 5.14e-109 308
MS.gene90793.t1 MTR_1g116170 85.714 133 15 2 1 133 1 129 4.97e-77 226
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene90793.t1 AT2G34860 56.069 173 64 4 15 175 14 186 6.15e-59 182
MS.gene90793.t1 AT2G34860 56.069 173 64 4 15 175 14 186 6.15e-59 182

Find 40 sgRNAs with CRISPR-Local

Find 49 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACATACCTTCTTCTAAGAAT+TGG 0.272269 1.4:+88155735 None:intergenic
CTTGTCTGCCCTGTTTGTTT+AGG 0.322236 1.4:-88155844 MS.gene90793:CDS
ACTGAATGTCCTAATTGTTA+TGG 0.323214 1.4:-88155877 MS.gene90793:CDS
CGGAATGGATTGCAATTTGC+AGG 0.323737 1.4:-88156457 None:intergenic
CTGAGAAGACCTTTATTGTT+TGG 0.336791 1.4:+88155811 None:intergenic
TTTCCTCGACCATAACAATT+AGG 0.345884 1.4:+88155868 None:intergenic
TGCCCTGTTTGTTTAGGAAC+TGG 0.352275 1.4:-88155838 MS.gene90793:CDS
CTGCTTGATAAGATGTATAA+TGG 0.353896 1.4:-88155769 MS.gene90793:CDS
GAAAATGGAAAGCTCTTAAC+AGG 0.369385 1.4:-88155927 MS.gene90793:CDS
TCAAGCAGTTTCTTTGCATC+AGG 0.377519 1.4:+88155784 None:intergenic
CATACCTTCTTCTAAGAATT+GGG 0.381922 1.4:+88155736 None:intergenic
TGTGTGGTGGTACAGGAAAA+TGG 0.392406 1.4:-88155942 MS.gene90793:CDS
ACTGGTGTGCCAAACAATAA+AGG 0.405844 1.4:-88155820 MS.gene90793:CDS
ACACCAGTTCCTAAACAAAC+AGG 0.409014 1.4:+88155835 None:intergenic
AGTTGGAAGAGGAGGAAGAG+TGG 0.470888 1.4:+88156331 None:intergenic
ACCGCTCTTGATAAACCTCC+AGG 0.498198 1.4:-88156122 MS.gene90793:CDS
CCACCACAATTTCTGCAACC+TGG 0.498937 1.4:+88156104 None:intergenic
GTTTGTTTGATAAAAGAAGT+TGG 0.500841 1.4:+88156408 None:intergenic
TCCACTCTTCCTCAAAATCA+AGG 0.504399 1.4:-88156357 MS.gene90793:CDS
CAAACAATAAAGGTCTTCTC+AGG 0.512552 1.4:-88155810 MS.gene90793:CDS
GGTGATATGTGTGGTGGTAC+AGG 0.517437 1.4:-88155949 MS.gene90793:CDS
TGCAGAAATTGTGGTGGAAG+TGG 0.524731 1.4:-88156098 MS.gene90793:CDS
TCTTCTAAGAATTGGGAAGT+AGG 0.526104 1.4:+88155743 None:intergenic
ACATACCTTGGAGTTGGAAG+AGG 0.537745 1.4:+88156320 None:intergenic
TGATAAAAGAAGTTGGAGGA+AGG 0.545348 1.4:+88156415 None:intergenic
TGTCCTAATTGTTATGGTCG+AGG 0.554383 1.4:-88155871 MS.gene90793:CDS
CCTCCAGGTTGCAGAAATTG+TGG 0.558014 1.4:-88156107 MS.gene90793:CDS
ATACATACATACCTTGGAGT+TGG 0.560487 1.4:+88156314 None:intergenic
ACATACATACATACATACCT+TGG 0.567889 1.4:+88156308 None:intergenic
TACCTTGGAGTTGGAAGAGG+AGG 0.582188 1.4:+88156323 None:intergenic
TTCCTCCTCTTCCAACTCCA+AGG 0.584619 1.4:-88156325 MS.gene90793:CDS
ACCTGGAGGTTTATCAAGAG+CGG 0.598366 1.4:+88156121 None:intergenic
CATTTAGGTGATATGTGTGG+TGG 0.616105 1.4:-88155955 MS.gene90793:intron
ACAATAAAGGTCTTCTCAGG+AGG 0.622506 1.4:-88155807 MS.gene90793:CDS
CCACAATTTCTGCAACCTGG+AGG 0.630868 1.4:+88156107 None:intergenic
CCAGGTTGCAGAAATTGTGG+TGG 0.631837 1.4:-88156104 MS.gene90793:CDS
TTTCATTTAGGTGATATGTG+TGG 0.652582 1.4:-88155958 MS.gene90793:intron
CACCAGTTCCTAAACAAACA+GGG 0.654127 1.4:+88155836 None:intergenic
TGTTTGATAAAAGAAGTTGG+AGG 0.669703 1.4:+88156411 None:intergenic
GAAGTTGGAGGAAGGCATCG+AGG 0.706960 1.4:+88156423 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTTGTTGCATTAATTAAAAA+TGG + chr1.4:88156048-88156067 None:intergenic 15.0%
!!! TACTACTTTTGATTTCATTT+AGG - chr1.4:88156230-88156249 MS.gene90793:intron 20.0%
! ATATCATATGATATGAATGC+AGG - chr1.4:88155948-88155967 MS.gene90793:CDS 25.0%
! GTTTGTTTGATAAAAGAAGT+TGG + chr1.4:88155795-88155814 None:intergenic 25.0%
!! ACTTCTTTTATCAAACAAAC+AGG - chr1.4:88155795-88155814 MS.gene90793:CDS 25.0%
ACATACATACATACATACCT+TGG + chr1.4:88155895-88155914 None:intergenic 30.0%
ACTGAATGTCCTAATTGTTA+TGG - chr1.4:88156323-88156342 MS.gene90793:CDS 30.0%
CTGCTTGATAAGATGTATAA+TGG - chr1.4:88156431-88156450 MS.gene90793:CDS 30.0%
TGATTATGAGAAAAGAGAAG+AGG + chr1.4:88156145-88156164 None:intergenic 30.0%
TGTTTGATAAAAGAAGTTGG+AGG + chr1.4:88155792-88155811 None:intergenic 30.0%
TTTCATTTAGGTGATATGTG+TGG - chr1.4:88156242-88156261 MS.gene90793:intron 30.0%
! CTTTTATCAAACAAACAGGA+AGG - chr1.4:88155799-88155818 MS.gene90793:CDS 30.0%
ATACATACATACCTTGGAGT+TGG + chr1.4:88155889-88155908 None:intergenic 35.0%
CAAACAATAAAGGTCTTCTC+AGG - chr1.4:88156390-88156409 MS.gene90793:CDS 35.0%
CTGAGAAGACCTTTATTGTT+TGG + chr1.4:88156392-88156411 None:intergenic 35.0%
GAAAATGGAAAGCTCTTAAC+AGG - chr1.4:88156273-88156292 MS.gene90793:intron 35.0%
TGATAAAAGAAGTTGGAGGA+AGG + chr1.4:88155788-88155807 None:intergenic 35.0%
TTTCCTCGACCATAACAATT+AGG + chr1.4:88156335-88156354 None:intergenic 35.0%
ACAATAAAGGTCTTCTCAGG+AGG - chr1.4:88156393-88156412 MS.gene90793:CDS 40.0%
ACACCAGTTCCTAAACAAAC+AGG + chr1.4:88156368-88156387 None:intergenic 40.0%
ACTGGTGTGCCAAACAATAA+AGG - chr1.4:88156380-88156399 MS.gene90793:CDS 40.0%
CACCAGTTCCTAAACAAACA+GGG + chr1.4:88156367-88156386 None:intergenic 40.0%
CATTTAGGTGATATGTGTGG+TGG - chr1.4:88156245-88156264 MS.gene90793:intron 40.0%
GCCTGGAAACAACATTTACT+GGG + chr1.4:88155996-88156015 None:intergenic 40.0%
GGAAGAAGAAGAAGAAGAAG+TGG - chr1.4:88155969-88155988 MS.gene90793:intron 40.0%
TCAAGCAGTTTCTTTGCATC+AGG + chr1.4:88156419-88156438 None:intergenic 40.0%
TCCACTCTTCCTCAAAATCA+AGG - chr1.4:88155843-88155862 MS.gene90793:CDS 40.0%
TGTCCTAATTGTTATGGTCG+AGG - chr1.4:88156329-88156348 MS.gene90793:CDS 40.0%
! ACATTTAAGACATTGACGCC+TGG + chr1.4:88156013-88156032 None:intergenic 40.0%
!! TCCCAGTAAATGTTGTTTCC+AGG - chr1.4:88155992-88156011 MS.gene90793:intron 40.0%
!! TCCTTGATTTTGAGGAAGAG+TGG + chr1.4:88155847-88155866 None:intergenic 40.0%
ACATACCTTGGAGTTGGAAG+AGG + chr1.4:88155883-88155902 None:intergenic 45.0%
ACCTGGAGGTTTATCAAGAG+CGG + chr1.4:88156082-88156101 None:intergenic 45.0%
CGCCTGGAAACAACATTTAC+TGG + chr1.4:88155997-88156016 None:intergenic 45.0%
CTTGTCTGCCCTGTTTGTTT+AGG - chr1.4:88156356-88156375 MS.gene90793:CDS 45.0%
TGCCCTGTTTGTTTAGGAAC+TGG - chr1.4:88156362-88156381 MS.gene90793:CDS 45.0%
TGTGTGGTGGTACAGGAAAA+TGG - chr1.4:88156258-88156277 MS.gene90793:intron 45.0%
! GAGTGGTGTCCTTGATTTTG+AGG + chr1.4:88155855-88155874 None:intergenic 45.0%
! TGCAGAAATTGTGGTGGAAG+TGG - chr1.4:88156102-88156121 MS.gene90793:CDS 45.0%
ACCGCTCTTGATAAACCTCC+AGG - chr1.4:88156078-88156097 MS.gene90793:intron 50.0%
AGTTGGAAGAGGAGGAAGAG+TGG + chr1.4:88155872-88155891 None:intergenic 50.0%
CCACAATTTCTGCAACCTGG+AGG + chr1.4:88156096-88156115 None:intergenic 50.0%
CCACCACAATTTCTGCAACC+TGG + chr1.4:88156099-88156118 None:intergenic 50.0%
CCAGGTTGCAGAAATTGTGG+TGG - chr1.4:88156096-88156115 MS.gene90793:CDS 50.0%
CCTCCAGGTTGCAGAAATTG+TGG - chr1.4:88156093-88156112 MS.gene90793:CDS 50.0%
GGTGATATGTGTGGTGGTAC+AGG - chr1.4:88156251-88156270 MS.gene90793:intron 50.0%
TACCTTGGAGTTGGAAGAGG+AGG + chr1.4:88155880-88155899 None:intergenic 50.0%
TTCCTCCTCTTCCAACTCCA+AGG - chr1.4:88155875-88155894 MS.gene90793:CDS 50.0%
!! GAAGTTGGAGGAAGGCATCG+AGG + chr1.4:88155780-88155799 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr1.4 gene 88155747 88156475 88155747 ID=MS.gene90793
chr1.4 mRNA 88155747 88156475 88155747 ID=MS.gene90793.t1;Parent=MS.gene90793
chr1.4 exon 88156294 88156475 88156294 ID=MS.gene90793.t1.exon1;Parent=MS.gene90793.t1
chr1.4 CDS 88156294 88156475 88156294 ID=cds.MS.gene90793.t1;Parent=MS.gene90793.t1
chr1.4 exon 88156087 88156208 88156087 ID=MS.gene90793.t1.exon2;Parent=MS.gene90793.t1
chr1.4 CDS 88156087 88156208 88156087 ID=cds.MS.gene90793.t1;Parent=MS.gene90793.t1
chr1.4 exon 88155747 88155970 88155747 ID=MS.gene90793.t1.exon3;Parent=MS.gene90793.t1
chr1.4 CDS 88155747 88155970 88155747 ID=cds.MS.gene90793.t1;Parent=MS.gene90793.t1
Gene Sequence

>MS.gene90793

ATGGATTGCAATTTGCAGGCGTCTGTTATTCCTCGATGCCTTCCTCCAACTTCTTTTATCAAACAAACAGGAAGGACGCGTTCTTTTATTGTTAAATCCACTCTTCCTCAAAATCAAGGACACCACTCTTCCTCCTCTTCCAACTCCAAGGTATGTATGTATGTATGTATGTTTATATACGAGTTTCAATTAACTTAATTAATATCATATGATATGAATGCAGGAAGAAGAAGAAGAAGAAGTGGTCCCAGTAAATGTTGTTTCCAGGCGTCAATGTCTTAAATGTTTATGTTCAACTCCATTTTTAATTAATGCAACAAACGCAGCAGCAACCGCTCTTGATAAACCTCCAGGTTGCAGAAATTGTGGTGGAAGTGGTAACATCATCTGTGAGTCCTCTTCTCTTTTCTCATAATCAAAATACTTTTATAATATTCTTGCTTATTATTATAATAATAATAATAATAATAATAATAATGAAAATACTACTTTTGATTTCATTTAGGTGATATGTGTGGTGGTACAGGAAAATGGAAAGCTCTTAACAGGAAGCGAGCACAAGATGTTTATGAGTTTACTGAATGTCCTAATTGTTATGGTCGAGGAAAGCTTGTCTGCCCTGTTTGTTTAGGAACTGGTGTGCCAAACAATAAAGGTCTTCTCAGGAGGCCTGATGCAAAGAAACTGCTTGATAAGATGTATAATGGACGCCTACTTCCCAATTCTTAG

Protein sequence

>MS.gene90793.t1

MDCNLQASVIPRCLPPTSFIKQTGRTRSFIVKSTLPQNQGHHSSSSSNSKVCMYVCMFIYERQCLKCLCSTPFLINATNAAATALDKPPGCRNCGGSGNIICDMCGGTGKWKALNRKRAQDVYEFTECPNCYGRGKLVCPVCLGTGVPNNKGLLRRPDAKKLLDKMYNGRLLPNS