Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0868550 | AFK43527.1 | 90.741 | 54 | 4 | 1 | 15 | 67 | 2 | 55 | 1.76e-22 | 93.2 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0868550 | I3STD5 | 90.741 | 54 | 4 | 1 | 15 | 67 | 2 | 55 | 8.42e-23 | 93.2 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0025340 | Msa0868550 | 0.800985 | 1.114413e-48 | -8.615850e-47 |
| Msa0028450 | Msa0868550 | 0.802847 | 4.620138e-49 | -8.615850e-47 |
| Msa0028940 | Msa0868550 | 0.800883 | 1.169524e-48 | -8.615850e-47 |
| Msa0046090 | Msa0868550 | 0.804652 | 1.949096e-49 | -8.615850e-47 |
| Msa0067820 | Msa0868550 | 0.804776 | 1.836381e-49 | -8.615850e-47 |
| Msa0093830 | Msa0868550 | 0.801611 | 8.296170e-49 | -8.615850e-47 |
| Msa0606230 | Msa0868550 | 0.811488 | 6.826641e-51 | -8.615850e-47 |
| Msa0616570 | Msa0868550 | 0.810170 | 1.316605e-50 | -8.615850e-47 |
| Msa0623350 | Msa0868550 | 0.810453 | 1.143967e-50 | -8.615850e-47 |
| Msa0624260 | Msa0868550 | 0.810240 | 1.271664e-50 | -8.615850e-47 |
| Msa0624360 | Msa0868550 | 0.809295 | 2.031117e-50 | -8.615850e-47 |
| Msa0649140 | Msa0868550 | 0.824553 | 7.582944e-54 | -8.615850e-47 |
| Msa0660160 | Msa0868550 | 0.810598 | 1.064271e-50 | -8.615850e-47 |
| Msa0667710 | Msa0868550 | 0.812923 | 3.319798e-51 | -8.615850e-47 |
| Msa0694810 | Msa0868550 | 0.811729 | 6.052931e-51 | -8.615850e-47 |
| Msa0699900 | Msa0868550 | 0.807650 | 4.556024e-50 | -8.615850e-47 |
| Msa0708450 | Msa0868550 | 0.801027 | 1.092757e-48 | -8.615850e-47 |
| Msa0748470 | Msa0868550 | 0.813438 | 2.560184e-51 | -8.615850e-47 |
| Msa0831710 | Msa0868550 | 0.803456 | 3.456207e-49 | -8.615850e-47 |
| Msa0147070 | Msa0868550 | 0.810472 | 1.133358e-50 | -8.615850e-47 |
| Msa0170550 | Msa0868550 | 0.813311 | 2.728939e-51 | -8.615850e-47 |
| Msa0195850 | Msa0868550 | 0.804582 | 2.016061e-49 | -8.615850e-47 |
| Msa0196620 | Msa0868550 | 0.819721 | 1.001119e-52 | -8.615850e-47 |
| Msa0231180 | Msa0868550 | 0.800860 | 1.182112e-48 | -8.615850e-47 |
| Msa0371050 | Msa0868550 | 0.813492 | 2.491089e-51 | -8.615850e-47 |
| Msa0384570 | Msa0868550 | 0.814640 | 1.390017e-51 | -8.615850e-47 |
| Msa0431310 | Msa0868550 | 0.808374 | 3.196501e-50 | -8.615850e-47 |
| Msa0439130 | Msa0868550 | 0.807225 | 5.608084e-50 | -8.615850e-47 |
| Msa0475080 | Msa0868550 | 0.810968 | 8.851301e-51 | -8.615850e-47 |
| Msa0275260 | Msa0868550 | 0.818723 | 1.688823e-52 | -8.615850e-47 |
| Msa0284980 | Msa0868550 | 0.819727 | 9.979345e-53 | -8.615850e-47 |
| Msa0305810 | Msa0868550 | 0.803178 | 3.946407e-49 | -8.615850e-47 |
| Msa0522510 | Msa0868550 | 0.804051 | 2.600947e-49 | -8.615850e-47 |
| Msa0532910 | Msa0868550 | 0.809485 | 1.848702e-50 | -8.615850e-47 |
| Msa0556690 | Msa0868550 | 0.807240 | 5.567455e-50 | -8.615850e-47 |
| Msa0562040 | Msa0868550 | 0.809736 | 1.633000e-50 | -8.615850e-47 |
| Msa0562740 | Msa0868550 | 0.803912 | 2.779315e-49 | -8.615850e-47 |
| Msa0565930 | Msa0868550 | 0.805043 | 1.615095e-49 | -8.615850e-47 |
| Msa0583810 | Msa0868550 | 0.801832 | 7.474604e-49 | -8.615850e-47 |
| Msa0868550 | Msa0868570 | 0.959082 | 4.732987e-117 | -8.615850e-47 |
| Msa0868550 | Msa0880200 | 0.811016 | 8.641968e-51 | -8.615850e-47 |
| Msa0868550 | Msa0883880 | 0.800378 | 1.482226e-48 | -8.615850e-47 |
| Msa0868550 | Msa0940400 | 0.807955 | 3.924710e-50 | -8.615850e-47 |
| Msa0868550 | Msa0985570 | 0.809312 | 2.014138e-50 | -8.615850e-47 |
| Msa0868550 | Msa1044520 | 0.815650 | 8.290521e-52 | -8.615850e-47 |
| Msa0868550 | Msa1066930 | 0.806452 | 8.168345e-50 | -8.615850e-47 |
| Msa0868550 | Msa1070710 | 0.809738 | 1.631252e-50 | -8.615850e-47 |
| Msa0868550 | Msa1118890 | 0.802245 | 6.147463e-49 | -8.615850e-47 |
| Msa0868550 | Msa1126980 | 0.803653 | 3.145386e-49 | -8.615850e-47 |
| Msa0868550 | Msa1127530 | 0.813437 | 2.560914e-51 | -8.615850e-47 |
| Msa0868550 | Msa1134840 | 0.806386 | 8.437226e-50 | -8.615850e-47 |
| Msa0868550 | Msa1154130 | 0.802205 | 6.266650e-49 | -8.615850e-47 |
| Msa0868550 | Msa1165330 | 0.809325 | 2.000791e-50 | -8.615850e-47 |
| Msa0868550 | Msa1177300 | 0.810909 | 9.118066e-51 | -8.615850e-47 |
| Msa0868550 | Msa1190300 | 0.800591 | 1.341283e-48 | -8.615850e-47 |
| Msa0868550 | Msa1190670 | 0.810138 | 1.338166e-50 | -8.615850e-47 |
| Msa0868550 | Msa1197210 | 0.807998 | 3.842948e-50 | -8.615850e-47 |
| Msa0868550 | Msa1223050 | 0.827076 | 1.909662e-54 | -8.615850e-47 |
| Msa0868550 | Msa1223520 | 0.808081 | 3.690008e-50 | -8.615850e-47 |
| Msa0868550 | Msa1235880 | 0.800937 | 1.140083e-48 | -8.615850e-47 |
| Msa0868550 | Msa1236090 | 0.819005 | 1.457050e-52 | -8.615850e-47 |
| Msa0868550 | Msa1244940 | 0.809073 | 2.265864e-50 | -8.615850e-47 |
| Msa0868550 | Msa1251890 | 0.804199 | 2.422633e-49 | -8.615850e-47 |
| Msa0868550 | Msa1263460 | 0.801753 | 7.760729e-49 | -8.615850e-47 |
| Msa0868550 | Msa1263600 | 0.822020 | 2.961618e-53 | -8.615850e-47 |
| Msa0868550 | Msa1264300 | 0.810651 | 1.036997e-50 | -8.615850e-47 |
| Msa0868550 | Msa1315990 | 0.811606 | 6.435828e-51 | -8.615850e-47 |
| Msa0868550 | Msa1374610 | 0.805657 | 1.200909e-49 | -8.615850e-47 |
| Msa0868550 | Msa1387650 | 0.805127 | 1.550552e-49 | -8.615850e-47 |
| Msa0868550 | Msa1405990 | 0.805024 | 1.630112e-49 | -8.615850e-47 |
| Msa0868550 | Msa1420630 | 0.810392 | 1.179143e-50 | -8.615850e-47 |
| Msa0868550 | Msa1425570 | 0.811056 | 8.473420e-51 | -8.615850e-47 |
| Msa0868550 | Msa1444750 | 0.814587 | 1.427714e-51 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 32 sgRNAs with CRISPR-Local
Find 49 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGGGATAGAGGTTTGTTCTT+GGG | 0.240869 | 6_1:-2141255 | Msa0868560:CDS |
| CCTTATGCGGTTGATGAAAT+TGG | 0.244779 | 6_1:-2141471 | Msa0868560:CDS |
| CCAAAGGTTCAGCCACTAAA+TGG | 0.299723 | 6_1:+2141415 | Msa0868550:CDS |
| GGGTAAAGTTAATGAGTTTA+GGG | 0.308790 | 6_1:-2141275 | Msa0868560:CDS |
| AGGGGATAGAGGTTTGTTCT+TGG | 0.343355 | 6_1:-2141256 | Msa0868560:CDS |
| TGGGTAAAGTTAATGAGTTT+AGG | 0.351146 | 6_1:-2141276 | Msa0868560:CDS |
| CCAATTTCATCAACCGCATA+AGG | 0.356856 | 6_1:+2141471 | Msa0868550:CDS |
| CCATTTAGTGGCTGAACCTT+TGG | 0.364491 | 6_1:-2141415 | Msa0868560:CDS |
| GGGATAGAGGTTTGTTCTTG+GGG | 0.405491 | 6_1:-2141254 | Msa0868560:CDS |
| GATGGGGATTTGTTGTTAGC+AGG | 0.421119 | 6_1:-2141357 | Msa0868560:CDS |
| GCTGAACCTTTGGTTGGTGG+TGG | 0.421703 | 6_1:-2141405 | Msa0868560:CDS |
| TTAATGAGTTTAGGGGATAG+AGG | 0.427839 | 6_1:-2141267 | Msa0868560:CDS |
| AGACTCAAGTGTCCATTTAG+TGG | 0.456450 | 6_1:-2141427 | Msa0868560:CDS |
| AACGATCACACAATTCCCTT+TGG | 0.471989 | 6_1:+2141188 | Msa0868550:CDS |
| CAATTTCATCAACCGCATAA+GGG | 0.479592 | 6_1:+2141472 | Msa0868550:CDS |
| GTGGCTGAACCTTTGGTTGG+TGG | 0.479791 | 6_1:-2141408 | Msa0868560:CDS |
| AATTCTTAGTGGAGAGTGAT+GGG | 0.480200 | 6_1:-2141374 | Msa0868560:CDS |
| ACTAAATGGACACTTGAGTC+TGG | 0.486588 | 6_1:+2141429 | Msa0868550:CDS |
| AAATTCTTAGTGGAGAGTGA+TGG | 0.487377 | 6_1:-2141375 | Msa0868560:CDS |
| TGAAATTGGTAAGACAGTTA+TGG | 0.491867 | 6_1:-2141457 | Msa0868560:CDS |
| CTGAACCTTTGGTTGGTGGT+GGG | 0.492608 | 6_1:-2141404 | Msa0868560:CDS |
| CTGATTTGTATGTTGCCAAA+GGG | 0.496509 | 6_1:-2141203 | Msa0868560:CDS |
| TGGGAAGAAGAAATTCTTAG+TGG | 0.503235 | 6_1:-2141385 | Msa0868560:CDS |
| TTTGTTTAAGCTTAATGAGA+AGG | 0.523648 | 6_1:-2141307 | Msa0868560:CDS |
| TCTGATTTGTATGTTGCCAA+AGG | 0.533512 | 6_1:-2141204 | Msa0868560:CDS |
| TTAGTGGCTGAACCTTTGGT+TGG | 0.535767 | 6_1:-2141411 | Msa0868560:CDS |
| TTAATGAGAAGGAGAAGAAG+TGG | 0.537156 | 6_1:-2141296 | Msa0868560:CDS |
| TAATGAGAAGGAGAAGAAGT+GGG | 0.568007 | 6_1:-2141295 | Msa0868560:CDS |
| TTCTTCCCACCACCAACCAA+AGG | 0.575003 | 6_1:+2141399 | Msa0868550:CDS |
| ATTGGTAAGACAGTTATGGT+TGG | 0.599573 | 6_1:-2141453 | Msa0868560:CDS |
| ATTCTTAGTGGAGAGTGATG+GGG | 0.629858 | 6_1:-2141373 | Msa0868560:CDS |
| GGTAAAGTTAATGAGTTTAG+GGG | 0.645059 | 6_1:-2141274 | Msa0868560:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AATCAAATAGAAAGTATACA+CGG | - | chr6_1:2140857-2140876 | None:intergenic | 20.0% |
| !! | ATGTGTATCATTCAAATTAA+TGG | + | chr6_1:2140890-2140909 | Msa0868550:intron | 20.0% |
| !! | AAATTAATGGAAAATGTGTA+GGG | + | chr6_1:2140903-2140922 | Msa0868550:intron | 20.0% |
| ! | CAAATTAATGGAAAATGTGT+AGG | + | chr6_1:2140902-2140921 | Msa0868550:intron | 25.0% |
| ! | ATGTGTAGGGTAAAAATTAA+TGG | + | chr6_1:2140916-2140935 | Msa0868550:intron | 25.0% |
| ! | TTTGTTTAAGCTTAATGAGA+AGG | - | chr6_1:2141310-2141329 | Msa0868560:CDS | 25.0% |
| CAAGATAACAATAACATACC+TGG | + | chr6_1:2141016-2141035 | Msa0868550:intron | 30.0% | |
| GGTAAAGTTAATGAGTTTAG+GGG | - | chr6_1:2141277-2141296 | Msa0868560:CDS | 30.0% | |
| GGGTAAAGTTAATGAGTTTA+GGG | - | chr6_1:2141278-2141297 | Msa0868560:CDS | 30.0% | |
| TGGGTAAAGTTAATGAGTTT+AGG | - | chr6_1:2141279-2141298 | Msa0868560:CDS | 30.0% | |
| TGAAATTGGTAAGACAGTTA+TGG | - | chr6_1:2141460-2141479 | Msa0868560:CDS | 30.0% | |
| ! | TTGGATCTGATTTTGTTACC+AGG | - | chr6_1:2141037-2141056 | None:intergenic | 35.0% |
| CAAAATCAGATCCAATTTCG+AGG | + | chr6_1:2141042-2141061 | Msa0868550:intron | 35.0% | |
| ! | CTGATTTGTATGTTGCCAAA+GGG | - | chr6_1:2141206-2141225 | Msa0868560:CDS | 35.0% |
| ! | TCTGATTTGTATGTTGCCAA+AGG | - | chr6_1:2141207-2141226 | Msa0868560:CDS | 35.0% |
| TTAATGAGTTTAGGGGATAG+AGG | - | chr6_1:2141270-2141289 | Msa0868560:CDS | 35.0% | |
| TAATGAGAAGGAGAAGAAGT+GGG | - | chr6_1:2141298-2141317 | Msa0868560:CDS | 35.0% | |
| TTAATGAGAAGGAGAAGAAG+TGG | - | chr6_1:2141299-2141318 | Msa0868560:CDS | 35.0% | |
| ! | TGTTGATGCGTTTTCTGTAA+GGG | - | chr6_1:2141337-2141356 | Msa0868560:CDS | 35.0% |
| AATTCTTAGTGGAGAGTGAT+GGG | - | chr6_1:2141377-2141396 | Msa0868560:CDS | 35.0% | |
| AAATTCTTAGTGGAGAGTGA+TGG | - | chr6_1:2141378-2141397 | Msa0868560:CDS | 35.0% | |
| TGGGAAGAAGAAATTCTTAG+TGG | - | chr6_1:2141388-2141407 | Msa0868560:CDS | 35.0% | |
| ATTGGTAAGACAGTTATGGT+TGG | - | chr6_1:2141456-2141475 | Msa0868560:CDS | 35.0% | |
| AATCAGATCCAATTTCGAGG+TGG | + | chr6_1:2141045-2141064 | Msa0868550:intron | 40.0% | |
| ! | TTTTGGACTTGGATCAAGGT+CGG | - | chr6_1:2141116-2141135 | Msa0868560:CDS | 40.0% |
| !! | TGTGTTTTGGACTTGGATCA+AGG | - | chr6_1:2141120-2141139 | Msa0868560:CDS | 40.0% |
| TAAGAAGACTAAGTCGACCT+GGG | - | chr6_1:2141155-2141174 | Msa0868560:CDS | 40.0% | |
| TTAAGAAGACTAAGTCGACC+TGG | - | chr6_1:2141156-2141175 | Msa0868560:CDS | 40.0% | |
| AACGATCACACAATTCCCTT+TGG | + | chr6_1:2141188-2141207 | Msa0868550:CDS | 40.0% | |
| !! | GTGTTGATGCGTTTTCTGTA+AGG | - | chr6_1:2141338-2141357 | Msa0868560:CDS | 40.0% |
| ATTCTTAGTGGAGAGTGATG+GGG | - | chr6_1:2141376-2141395 | Msa0868560:CDS | 40.0% | |
| AGACTCAAGTGTCCATTTAG+TGG | - | chr6_1:2141430-2141449 | Msa0868560:CDS | 40.0% | |
| ACTAAATGGACACTTGAGTC+TGG | + | chr6_1:2141429-2141448 | Msa0868550:CDS | 40.0% | |
| TGTTCTGTCCACCTCGAAAT+TGG | - | chr6_1:2141056-2141075 | Msa0868560:CDS | 45.0% | |
| !! | TGAGAGCTGTGTTTTGGACT+TGG | - | chr6_1:2141127-2141146 | Msa0868560:CDS | 45.0% |
| ! | GTCACATGAGAGCTGTGTTT+TGG | - | chr6_1:2141133-2141152 | Msa0868560:CDS | 45.0% |
| ! | GGGATAGAGGTTTGTTCTTG+GGG | - | chr6_1:2141257-2141276 | Msa0868560:CDS | 45.0% |
| ! | GGGGATAGAGGTTTGTTCTT+GGG | - | chr6_1:2141258-2141277 | Msa0868560:CDS | 45.0% |
| ! | AGGGGATAGAGGTTTGTTCT+TGG | - | chr6_1:2141259-2141278 | Msa0868560:CDS | 45.0% |
| !! | GATGGGGATTTGTTGTTAGC+AGG | - | chr6_1:2141360-2141379 | Msa0868560:CDS | 45.0% |
| TTAGTGGCTGAACCTTTGGT+TGG | - | chr6_1:2141414-2141433 | Msa0868560:CDS | 45.0% | |
| CCATTTAGTGGCTGAACCTT+TGG | - | chr6_1:2141418-2141437 | Msa0868560:CDS | 45.0% | |
| CCAAAGGTTCAGCCACTAAA+TGG | + | chr6_1:2141415-2141434 | Msa0868550:CDS | 45.0% | |
| TCGAGGTGGACAGAACAAGT+TGG | + | chr6_1:2141059-2141078 | Msa0868550:intron | 50.0% | |
| TTCTTCCCACCACCAACCAA+AGG | + | chr6_1:2141399-2141418 | Msa0868550:CDS | 50.0% | |
| !! | CTGAACCTTTGGTTGGTGGT+GGG | - | chr6_1:2141407-2141426 | Msa0868560:CDS | 50.0% |
| ACACAGCTCTCATGTGACCC+AGG | + | chr6_1:2141135-2141154 | Msa0868550:intron | 55.0% | |
| !! | GCTGAACCTTTGGTTGGTGG+TGG | - | chr6_1:2141408-2141427 | Msa0868560:CDS | 55.0% |
| GTGGCTGAACCTTTGGTTGG+TGG | - | chr6_1:2141411-2141430 | Msa0868560:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6_1 | gene | 2140826 | 2141491 | 2140826 | ID=Msa0868550;Name=Msa0868550 |
| chr6_1 | mRNA | 2140826 | 2141491 | 2140826 | ID=Msa0868550-mRNA-1;Parent=Msa0868550;Name=Msa0868550-mRNA-1;_AED=0.48;_eAED=0.53;_QI=0|0|0|0.5|1|1|2|0|113 |
| chr6_1 | exon | 2140826 | 2140832 | 2140826 | ID=Msa0868550-mRNA-1:exon:610;Parent=Msa0868550-mRNA-1 |
| chr6_1 | exon | 2141157 | 2141491 | 2141157 | ID=Msa0868550-mRNA-1:exon:611;Parent=Msa0868550-mRNA-1 |
| chr6_1 | CDS | 2140826 | 2140832 | 2140826 | ID=Msa0868550-mRNA-1:cds;Parent=Msa0868550-mRNA-1 |
| chr6_1 | CDS | 2141157 | 2141491 | 2141157 | ID=Msa0868550-mRNA-1:cds;Parent=Msa0868550-mRNA-1 |
| Gene Sequence |
| Protein sequence |
