AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0868570


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 25 sgRNAs with CRISPR-Local

Find 35 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTGTTAATTATTGAGGATTT+TGG 0.148562 6_1:+2143603 Msa0868570:CDS
GCTGAATAAGATTCCTCTAT+TGG 0.255868 6_1:+2143749 Msa0868570:CDS
TCTTCAAATATATTAAGTTT+TGG 0.295681 6_1:-2143660 None:intergenic
CAAAGTTGTTTCTTCATTGT+TGG 0.297204 6_1:-2143727 None:intergenic
ACTTAGATTCAGAACATATC+AGG 0.355882 6_1:+2143455 Msa0868570:CDS
TTTGATCCAGATTTGTTACT+TGG 0.376451 6_1:-2143937 None:intergenic
GCCTGGTCTGTTAATTATTG+AGG 0.400311 6_1:+2143596 Msa0868570:CDS
AACACTCCAAGTAACAAATC+TGG 0.416266 6_1:+2143931 Msa0868570:CDS
AGCGACGTCAGCCTTGTCAT+TGG 0.423496 6_1:-2143893 None:intergenic
GGATCAATCAACGGGACATA+AGG 0.431213 6_1:-2143639 None:intergenic
TAGGGAAATGCCCGCCTGCC+TGG 0.440332 6_1:+2143579 Msa0868570:intron
GTCTCTATCGGAAGTGGCAT+TGG 0.440777 6_1:-2143412 None:intergenic
TTTGTTAAACTTACCAATAG+AGG 0.443679 6_1:-2143762 None:intergenic
GGAAGTGGCATTGGCTTGAA+TGG 0.458677 6_1:-2143403 None:intergenic
AAAGATGTCTCTATCGGAAG+TGG 0.544294 6_1:-2143418 None:intergenic
CAATCAACGGGACATAAGGT+TGG 0.544757 6_1:-2143635 None:intergenic
GTTGATAAAGATGTCTCTAT+CGG 0.564645 6_1:-2143424 None:intergenic
AATTAACAGACCAGGCAGGC+GGG 0.580326 6_1:-2143589 None:intergenic
CAATAATTAACAGACCAGGC+AGG 0.599473 6_1:-2143593 None:intergenic
GGTGATAATCGCAATTTACA+CGG 0.601171 6_1:-2143381 None:intergenic
GAAGTGGCATTGGCTTGAAT+GGG 0.601775 6_1:-2143402 None:intergenic
TCCTCAATAATTAACAGACC+AGG 0.611424 6_1:-2143597 None:intergenic
TAATTAACAGACCAGGCAGG+CGG 0.628066 6_1:-2143590 None:intergenic
ACTTGCAATGAAGAAGATGA+TGG 0.638058 6_1:+2143699 Msa0868570:CDS
ATGTAGTGATTCCAATGACA+AGG 0.645229 6_1:+2143882 Msa0868570:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATCATAATATTTAGATCA+AGG - chr6_1:2143539-2143558 None:intergenic 15.0%
!!! TCTTCAAATATATTAAGTTT+TGG - chr6_1:2143663-2143682 None:intergenic 15.0%
!!! AAAATGTGTATTTTTGTCAT+AGG + chr6_1:2143560-2143579 Msa0868570:intron 20.0%
!!! AAATGTGTATTTTTGTCATA+GGG + chr6_1:2143561-2143580 Msa0868570:intron 20.0%
!! CTGTTAATTATTGAGGATTT+TGG + chr6_1:2143603-2143622 Msa0868570:CDS 25.0%
!!! TTAAGTTTTGGATCAATCAA+CGG - chr6_1:2143651-2143670 None:intergenic 25.0%
! TTTGTTAAACTTACCAATAG+AGG - chr6_1:2143765-2143784 None:intergenic 25.0%
! GTTGATAAAGATGTCTCTAT+CGG - chr6_1:2143427-2143446 None:intergenic 30.0%
ACTTAGATTCAGAACATATC+AGG + chr6_1:2143455-2143474 Msa0868570:CDS 30.0%
GTTTAGAAAAACACTTACGT+TGG - chr6_1:2143496-2143515 None:intergenic 30.0%
!!! TAAGTTTTGGATCAATCAAC+GGG - chr6_1:2143650-2143669 None:intergenic 30.0%
!! CAAAGTTGTTTCTTCATTGT+TGG - chr6_1:2143730-2143749 None:intergenic 30.0%
TAATACACCATACTATGTTC+AGG + chr6_1:2143849-2143868 Msa0868570:intron 30.0%
!! TTTGATCCAGATTTGTTACT+TGG - chr6_1:2143940-2143959 None:intergenic 30.0%
GGTGATAATCGCAATTTACA+CGG - chr6_1:2143384-2143403 None:intergenic 35.0%
AGAAAAACACTTACGTTGGT+AGG - chr6_1:2143492-2143511 None:intergenic 35.0%
TCCTCAATAATTAACAGACC+AGG - chr6_1:2143600-2143619 None:intergenic 35.0%
ACTTGCAATGAAGAAGATGA+TGG + chr6_1:2143699-2143718 Msa0868570:CDS 35.0%
GCTGAATAAGATTCCTCTAT+TGG + chr6_1:2143749-2143768 Msa0868570:CDS 35.0%
ATAGCTTCCTGAACATAGTA+TGG - chr6_1:2143859-2143878 None:intergenic 35.0%
ATGTAGTGATTCCAATGACA+AGG + chr6_1:2143882-2143901 Msa0868570:CDS 35.0%
!! ACTTGGAGTGTTTTCAATAC+TGG - chr6_1:2143923-2143942 None:intergenic 35.0%
AACACTCCAAGTAACAAATC+TGG + chr6_1:2143931-2143950 Msa0868570:CDS 35.0%
AAAGATGTCTCTATCGGAAG+TGG - chr6_1:2143421-2143440 None:intergenic 40.0%
CAATAATTAACAGACCAGGC+AGG - chr6_1:2143596-2143615 None:intergenic 40.0%
GCCTGGTCTGTTAATTATTG+AGG + chr6_1:2143596-2143615 Msa0868570:CDS 40.0%
!! GAAGTGGCATTGGCTTGAAT+GGG - chr6_1:2143405-2143424 None:intergenic 45.0%
TAATTAACAGACCAGGCAGG+CGG - chr6_1:2143593-2143612 None:intergenic 45.0%
CAATCAACGGGACATAAGGT+TGG - chr6_1:2143638-2143657 None:intergenic 45.0%
GGATCAATCAACGGGACATA+AGG - chr6_1:2143642-2143661 None:intergenic 45.0%
!! GGAAGTGGCATTGGCTTGAA+TGG - chr6_1:2143406-2143425 None:intergenic 50.0%
GTCTCTATCGGAAGTGGCAT+TGG - chr6_1:2143415-2143434 None:intergenic 50.0%
AATTAACAGACCAGGCAGGC+GGG - chr6_1:2143592-2143611 None:intergenic 50.0%
! AGCGACGTCAGCCTTGTCAT+TGG - chr6_1:2143896-2143915 None:intergenic 55.0%
TAGGGAAATGCCCGCCTGCC+TGG + chr6_1:2143579-2143598 Msa0868570:intron 65.0%
Chromosome Type Strat End Strand Name
chr6_1 gene 2143369 2143963 2143369 ID=Msa0868570;Name=Msa0868570
chr6_1 mRNA 2143369 2143963 2143369 ID=Msa0868570-mRNA-1;Parent=Msa0868570;Name=Msa0868570-mRNA-1;_AED=0.34;_eAED=0.35;_QI=0|0|0|0.66|1|1|3|0|136
chr6_1 exon 2143369 2143497 2143369 ID=Msa0868570-mRNA-1:exon:604;Parent=Msa0868570-mRNA-1
chr6_1 exon 2143582 2143770 2143582 ID=Msa0868570-mRNA-1:exon:605;Parent=Msa0868570-mRNA-1
chr6_1 exon 2143871 2143963 2143871 ID=Msa0868570-mRNA-1:exon:606;Parent=Msa0868570-mRNA-1
chr6_1 CDS 2143369 2143497 2143369 ID=Msa0868570-mRNA-1:cds;Parent=Msa0868570-mRNA-1
chr6_1 CDS 2143582 2143770 2143582 ID=Msa0868570-mRNA-1:cds;Parent=Msa0868570-mRNA-1
chr6_1 CDS 2143871 2143963 2143871 ID=Msa0868570-mRNA-1:cds;Parent=Msa0868570-mRNA-1
Gene Sequence

>Msa0868570

ATGCATGCTAAGCCGTGTAAATTGCGATTATCACCCATTCAAGCCAATGCCACTTCCGATAGAGACATCTTTATCAACTCACTCAAACTTAGATTCAGAACATATCAGGTTGAATTTGATCCTACCAACGGAAATGCCCGCCTGCCTGGTCTGTTAATTATTGAGGATTTTGGTTTCGAACTCCAACCTTATGTCCCGTTGATTGATCCAAAACTTAATATATTTGAAGAAATTGTTATCAAATACACTTGCAATGAAGAAGATGATGGTGAGTCCAACAATGAAGAAACAACTTTGCTGAATAAGATTCCTCTATTGGAAGCTATAGAATGTAGTGATTCCAATGACAAGGCTGACGTCGCTTTAACCAGTATTGAAAACACTCCAAGTAACAAATCTGGATCAAAATGA

Protein sequence

>Msa0868570

MHAKPCKLRLSPIQANATSDRDIFINSLKLRFRTYQVEFDPTNGNARLPGLLIIEDFGFELQPYVPLIDPKLNIFEEIVIKYTCNEEDDGESNNEETTLLNKIPLLEAIECSDSNDKADVALTSIENTPSNKSGSK*