Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1180700 | XP_003627044.1 | 94.934 | 454 | 22 | 1 | 1 | 453 | 1 | 454 | 0.0 | 801 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1180700 | G7LGP0 | 94.934 | 454 | 22 | 1 | 1 | 453 | 1 | 454 | 0.0 | 801 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0016040 | Msa1180700 | 0.815569 | 8.644358e-52 | -8.615850e-47 |
| Msa0030070 | Msa1180700 | 0.815006 | 1.152914e-51 | -8.615850e-47 |
| Msa0032980 | Msa1180700 | 0.815277 | 1.004107e-51 | -8.615850e-47 |
| Msa0040640 | Msa1180700 | 0.816731 | 4.753830e-52 | -8.615850e-47 |
| Msa0067380 | Msa1180700 | 0.812624 | 3.861490e-51 | -8.615850e-47 |
| Msa0090440 | Msa1180700 | 0.814110 | 1.820271e-51 | -8.615850e-47 |
| Msa0091350 | Msa1180700 | 0.818096 | 2.343180e-52 | -8.615850e-47 |
| Msa0106630 | Msa1180700 | 0.806052 | 9.918187e-50 | -8.615850e-47 |
| Msa0113410 | Msa1180700 | 0.809508 | 1.828104e-50 | -8.615850e-47 |
| Msa0607800 | Msa1180700 | 0.822899 | 1.850385e-53 | -8.615850e-47 |
| Msa0613630 | Msa1180700 | 0.829089 | 6.257149e-55 | -8.615850e-47 |
| Msa0620500 | Msa1180700 | 0.818503 | 1.895175e-52 | -8.615850e-47 |
| Msa0625620 | Msa1180700 | 0.810004 | 1.429760e-50 | -8.615850e-47 |
| Msa0640800 | Msa1180700 | 0.803789 | 2.948173e-49 | -8.615850e-47 |
| Msa0647050 | Msa1180700 | 0.805254 | 1.458506e-49 | -8.615850e-47 |
| Msa0647440 | Msa1180700 | 0.817002 | 4.132027e-52 | -8.615850e-47 |
| Msa0648040 | Msa1180700 | 0.814549 | 1.456023e-51 | -8.615850e-47 |
| Msa0648760 | Msa1180700 | 0.830041 | 3.671151e-55 | -8.615850e-47 |
| Msa0657210 | Msa1180700 | 0.820321 | 7.297884e-53 | -8.615850e-47 |
| Msa0690320 | Msa1180700 | 0.801685 | 8.011787e-49 | -8.615850e-47 |
| Msa0690740 | Msa1180700 | 0.820094 | 8.222367e-53 | -8.615850e-47 |
| Msa0691270 | Msa1180700 | 0.809385 | 1.942083e-50 | -8.615850e-47 |
| Msa0701250 | Msa1180700 | 0.832317 | 1.012623e-55 | -8.615850e-47 |
| Msa0707260 | Msa1180700 | 0.804565 | 2.032273e-49 | -8.615850e-47 |
| Msa0714520 | Msa1180700 | 0.811953 | 5.410200e-51 | -8.615850e-47 |
| Msa0717900 | Msa1180700 | 0.826091 | 3.281302e-54 | -8.615850e-47 |
| Msa0720280 | Msa1180700 | 0.802411 | 5.681490e-49 | -8.615850e-47 |
| Msa0745720 | Msa1180700 | 0.805577 | 1.248373e-49 | -8.615850e-47 |
| Msa0800660 | Msa1180700 | 0.807462 | 4.994739e-50 | -8.615850e-47 |
| Msa0816080 | Msa1180700 | 0.819251 | 1.281063e-52 | -8.615850e-47 |
| Msa0837680 | Msa1180700 | 0.804178 | 2.446672e-49 | -8.615850e-47 |
| Msa0125590 | Msa1180700 | 0.824336 | 8.527910e-54 | -8.615850e-47 |
| Msa0127340 | Msa1180700 | 0.804782 | 1.831207e-49 | -8.615850e-47 |
| Msa0127680 | Msa1180700 | 0.807412 | 5.120255e-50 | -8.615850e-47 |
| Msa0153210 | Msa1180700 | 0.801658 | 8.116968e-49 | -8.615850e-47 |
| Msa0158900 | Msa1180700 | 0.831670 | 1.463472e-55 | -8.615850e-47 |
| Msa0165830 | Msa1180700 | 0.834051 | 3.747597e-56 | -8.615850e-47 |
| Msa0170070 | Msa1180700 | 0.818185 | 2.237061e-52 | -8.615850e-47 |
| Msa0186180 | Msa1180700 | 0.824747 | 6.826791e-54 | -8.615850e-47 |
| Msa0373170 | Msa1180700 | 0.832667 | 8.294812e-56 | -8.615850e-47 |
| Msa0373590 | Msa1180700 | 0.801602 | 8.335032e-49 | -8.615850e-47 |
| Msa0382230 | Msa1180700 | 0.802687 | 4.983519e-49 | -8.615850e-47 |
| Msa0421130 | Msa1180700 | 0.804403 | 2.196839e-49 | -8.615850e-47 |
| Msa0421750 | Msa1180700 | 0.817493 | 3.204919e-52 | -8.615850e-47 |
| Msa0433760 | Msa1180700 | 0.834616 | 2.704120e-56 | -8.615850e-47 |
| Msa0466520 | Msa1180700 | 0.831286 | 1.819382e-55 | -8.615850e-47 |
| Msa0466880 | Msa1180700 | 0.808973 | 2.380033e-50 | -8.615850e-47 |
| Msa0467690 | Msa1180700 | 0.833611 | 4.829366e-56 | -8.615850e-47 |
| Msa0468700 | Msa1180700 | 0.800490 | 1.406620e-48 | -8.615850e-47 |
| Msa0477630 | Msa1180700 | 0.825901 | 3.639654e-54 | -8.615850e-47 |
| Msa0478140 | Msa1180700 | 0.839183 | 1.845456e-57 | -8.615850e-47 |
| Msa0478200 | Msa1180700 | 0.834766 | 2.479070e-56 | -8.615850e-47 |
| Msa0480730 | Msa1180700 | 0.812480 | 4.151271e-51 | -8.615850e-47 |
| Msa1000780 | Msa1180700 | 0.805844 | 1.096785e-49 | -8.615850e-47 |
| Msa1008670 | Msa1180700 | 0.817655 | 2.947069e-52 | -8.615850e-47 |
| Msa1008680 | Msa1180700 | 0.800514 | 1.390878e-48 | -8.615850e-47 |
| Msa1082380 | Msa1180700 | 0.800716 | 1.264906e-48 | -8.615850e-47 |
| Msa1106220 | Msa1180700 | 0.813741 | 2.195591e-51 | -8.615850e-47 |
| Msa0265310 | Msa1180700 | 0.800259 | 1.567270e-48 | -8.615850e-47 |
| Msa1148630 | Msa1180700 | 0.820384 | 7.055934e-53 | -8.615850e-47 |
| Msa1162880 | Msa1180700 | 0.805549 | 1.265252e-49 | -8.615850e-47 |
| Msa1180700 | Msa1188790 | 0.818851 | 1.579959e-52 | -8.615850e-47 |
| Msa1180700 | Msa1191000 | 0.807207 | 5.657233e-50 | -8.615850e-47 |
| Msa1180700 | Msa1215980 | 0.818653 | 1.751655e-52 | -8.615850e-47 |
| Msa1180700 | Msa1218820 | 0.815894 | 7.316191e-52 | -8.615850e-47 |
| Msa1180700 | Msa1231780 | 0.830027 | 3.699787e-55 | -8.615850e-47 |
| Msa1180700 | Msa1242960 | 0.807080 | 6.018804e-50 | -8.615850e-47 |
| Msa1180700 | Msa1254420 | 0.800251 | 1.572923e-48 | -8.615850e-47 |
| Msa1180700 | Msa1254540 | 0.830271 | 3.226792e-55 | -8.615850e-47 |
| Msa1180700 | Msa1263740 | 0.825871 | 3.699918e-54 | -8.615850e-47 |
| Msa1180700 | Msa1263780 | 0.807794 | 4.247213e-50 | -8.615850e-47 |
| Msa1180700 | Msa1267930 | 0.831666 | 1.467166e-55 | -8.615850e-47 |
| Msa1180700 | Msa1294460 | 0.827307 | 1.681678e-54 | -8.615850e-47 |
| Msa1180700 | Msa1294790 | 0.820855 | 5.501615e-53 | -8.615850e-47 |
| Msa1180700 | Msa1300460 | 0.812616 | 3.876047e-51 | -8.615850e-47 |
| Msa1180700 | Msa1305780 | 0.817069 | 3.992435e-52 | -8.615850e-47 |
| Msa1180700 | Msa1305920 | 0.804354 | 2.248748e-49 | -8.615850e-47 |
| Msa1180700 | Msa1332950 | 0.824544 | 7.622170e-54 | -8.615850e-47 |
| Msa1180700 | Msa1381470 | 0.837448 | 5.165761e-57 | -8.615850e-47 |
| Msa1180700 | Msa1387500 | 0.816628 | 5.012650e-52 | -8.615850e-47 |
| Msa1180700 | Msa1393560 | 0.823574 | 1.287246e-53 | -8.615850e-47 |
| Msa1180700 | Msa1399190 | 0.814474 | 1.512818e-51 | -8.615850e-47 |
| Msa1180700 | Msa1406720 | 0.829847 | 4.094031e-55 | -8.615850e-47 |
| Msa1180700 | Msa1426320 | 0.821307 | 4.329551e-53 | -8.615850e-47 |
| Msa1180700 | Msa1434640 | 0.801857 | 7.386456e-49 | -8.615850e-47 |
| Msa1180700 | Msa1442260 | 0.816580 | 5.140319e-52 | -8.615850e-47 |
| Msa1180700 | Msa1457840 | 0.829105 | 6.201703e-55 | -8.615850e-47 |
| Msa0503990 | Msa1180700 | 0.806369 | 8.507601e-50 | -8.615850e-47 |
| Msa0507810 | Msa1180700 | 0.804615 | 1.983715e-49 | -8.615850e-47 |
| Msa0507920 | Msa1180700 | 0.801268 | 9.754334e-49 | -8.615850e-47 |
| Msa0513080 | Msa1180700 | 0.804579 | 2.019167e-49 | -8.615850e-47 |
| Msa0514020 | Msa1180700 | 0.831601 | 1.521690e-55 | -8.615850e-47 |
| Msa0514500 | Msa1180700 | 0.800560 | 1.361197e-48 | -8.615850e-47 |
| Msa0520680 | Msa1180700 | 0.800103 | 1.685890e-48 | -8.615850e-47 |
| Msa0520870 | Msa1180700 | 0.809509 | 1.827019e-50 | -8.615850e-47 |
| Msa0521680 | Msa1180700 | 0.837002 | 6.719236e-57 | -8.615850e-47 |
| Msa0541740 | Msa1180700 | 0.832180 | 1.094878e-55 | -8.615850e-47 |
| Msa0550590 | Msa1180700 | 0.842619 | 2.315050e-58 | -8.615850e-47 |
| Msa0554300 | Msa1180700 | 0.813295 | 2.750981e-51 | -8.615850e-47 |
| Msa0563900 | Msa1180700 | 0.808865 | 2.509848e-50 | -8.615850e-47 |
| Msa0572830 | Msa1180700 | 0.815939 | 7.148154e-52 | -8.615850e-47 |
| Msa0586200 | Msa1180700 | 0.807237 | 5.576279e-50 | -8.615850e-47 |
| Msa0867110 | Msa1180700 | 0.803839 | 2.878471e-49 | -8.615850e-47 |
| Msa0879920 | Msa1180700 | 0.804720 | 1.886880e-49 | -8.615850e-47 |
| Msa0900090 | Msa1180700 | 0.843369 | 1.462150e-58 | -8.615850e-47 |
| Msa0909760 | Msa1180700 | 0.813357 | 2.667041e-51 | -8.615850e-47 |
| Msa0931310 | Msa1180700 | 0.803237 | 3.836176e-49 | -8.615850e-47 |
| Msa0965470 | Msa1180700 | 0.803073 | 4.148843e-49 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1180700 | MtrunA17_Chr8g0340711 | 94.934 | 454 | 22 | 1 | 1 | 453 | 1 | 454 | 0.0 | 801 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1180700 | AT4G12540.2 | 36.364 | 462 | 218 | 15 | 20 | 453 | 24 | 437 | 1.47e-66 | 220 |
| Msa1180700 | AT4G12540.5 | 36.364 | 462 | 218 | 15 | 20 | 453 | 24 | 437 | 1.47e-66 | 220 |
| Msa1180700 | AT4G12540.3 | 36.364 | 462 | 218 | 15 | 20 | 453 | 17 | 430 | 2.14e-66 | 219 |
| Msa1180700 | AT4G12540.1 | 34.864 | 479 | 219 | 16 | 20 | 453 | 24 | 454 | 1.48e-62 | 210 |
| Msa1180700 | AT4G12540.4 | 36.285 | 463 | 214 | 16 | 24 | 453 | 15 | 429 | 2.47e-62 | 209 |
Find 88 sgRNAs with CRISPR-Local
Find 149 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAACTCAGTGAAATCTAATT+TGG | 0.166349 | 8_1:-14908736 | None:intergenic |
| GAAAGTGAACTTTGTAATTT+TGG | 0.178876 | 8_1:-14908492 | None:intergenic |
| GCAAGCAAATAATATCTTAT+TGG | 0.199209 | 8_1:+14909767 | Msa1180700:three_prime_UTR |
| CTCCTTCGCCTCTTGTTTCA+TGG | 0.201608 | 8_1:+14908613 | Msa1180700:CDS |
| TCAAGCAAAGCACAAGATTT+TGG | 0.208841 | 8_1:-14908948 | None:intergenic |
| AGAGAAAGGGCAGGCTTTAT+TGG | 0.223544 | 8_1:-14908810 | None:intergenic |
| ATACTTTGAAGAAGGAATTA+TGG | 0.234678 | 8_1:+14909570 | Msa1180700:CDS |
| CACACGACATATTTCAGATA+TGG | 0.242872 | 8_1:-14908973 | None:intergenic |
| TGAAATGTTGTCGCGGTTAA+AGG | 0.258164 | 8_1:+14907815 | Msa1180700:CDS |
| AATAATGATTCAGTGCTTTA+CGG | 0.286271 | 8_1:+14908768 | Msa1180700:CDS |
| GATACTAATGTAGTCTCTTT+AGG | 0.311138 | 8_1:-14908591 | None:intergenic |
| GATAACAATGATTTCGATAA+TGG | 0.320197 | 8_1:-14908690 | None:intergenic |
| GTAAAGCACTGAATCATTAT+TGG | 0.331032 | 8_1:-14908766 | None:intergenic |
| TCAGAAGATTCTGAAGCTTC+TGG | 0.335637 | 8_1:+14909053 | Msa1180700:CDS |
| TCCTTTCCAATTGCTGACTT+TGG | 0.336920 | 8_1:-14909140 | None:intergenic |
| TACTTTGAAGAAGGAATTAT+GGG | 0.338127 | 8_1:+14909571 | Msa1180700:CDS |
| GAAAGGGTTTCTTGACCTAT+TGG | 0.342475 | 8_1:+14909661 | Msa1180700:CDS |
| TTGTCCAACGATTTCTCATC+AGG | 0.375325 | 8_1:-14909227 | None:intergenic |
| GGTTTGTGGTTGGTGTGGTT+TGG | 0.378722 | 8_1:-14908715 | None:intergenic |
| TTTGGCTTGATTATGACCTT+TGG | 0.381837 | 8_1:-14909339 | None:intergenic |
| TCTTTCCACTCTTCTTCATC+AGG | 0.384489 | 8_1:+14907529 | Msa1180700:CDS |
| CGATGGTGTGACGCAGGGTT+TGG | 0.387486 | 8_1:+14909079 | Msa1180700:CDS |
| TGTGTTCCAAAGTCAGCAAT+TGG | 0.392723 | 8_1:+14909134 | Msa1180700:CDS |
| ATTGACTTGTTGCTTAAGAA+TGG | 0.395329 | 8_1:-14909291 | None:intergenic |
| TAATGAACTCCAACAGGAAA+AGG | 0.401395 | 8_1:-14909023 | None:intergenic |
| CAATGTTTCCCCATGAGATC+TGG | 0.404517 | 8_1:-14909113 | None:intergenic |
| GTTTGAAACTTAGACTCAAA+TGG | 0.405729 | 8_1:-14909620 | None:intergenic |
| GGAGGTGAGGTAAGCCAAAC+AGG | 0.412175 | 8_1:-14909179 | None:intergenic |
| AAGAAGAGTGTAAGCAAAGA+GGG | 0.415838 | 8_1:+14907756 | Msa1180700:CDS |
| AATAAAGCCTGCCCTTTCTC+TGG | 0.439735 | 8_1:+14908812 | Msa1180700:CDS |
| ATGTTGAAGCATCACCTGTT+TGG | 0.440053 | 8_1:+14909165 | Msa1180700:CDS |
| CAAATTAGATTTCACTGAGT+TGG | 0.440495 | 8_1:+14908737 | Msa1180700:CDS |
| ACACGACATATTTCAGATAT+GGG | 0.441396 | 8_1:-14908972 | None:intergenic |
| GTGAAATCTAATTTGGTTTG+TGG | 0.455967 | 8_1:-14908729 | None:intergenic |
| GAGTGGAAAGATCGGAATGA+AGG | 0.459544 | 8_1:-14907517 | None:intergenic |
| AATCTAATTTGGTTTGTGGT+TGG | 0.462236 | 8_1:-14908725 | None:intergenic |
| CCTCTTCACTCACCAGAGAA+AGG | 0.463469 | 8_1:-14908824 | None:intergenic |
| TCACTGTAATGAACTCCAAC+AGG | 0.463669 | 8_1:-14909029 | None:intergenic |
| GATCGGAATGAAGGAGAGTG+AGG | 0.469677 | 8_1:-14907508 | None:intergenic |
| CACAGGAAGCTTCTTCCAAT+AGG | 0.469810 | 8_1:-14909676 | None:intergenic |
| AGGGCAGGCTTTATTGGAGT+TGG | 0.475554 | 8_1:-14908804 | None:intergenic |
| CAATAAATTGAATGTGGAAG+AGG | 0.483861 | 8_1:+14909310 | Msa1180700:CDS |
| AATGAACTCCAACAGGAAAA+GGG | 0.490414 | 8_1:-14909022 | None:intergenic |
| TGGCAGCGATGGTGTGACGC+AGG | 0.497686 | 8_1:+14909073 | Msa1180700:CDS |
| GCATGAATTGATTTCTTCGC+CGG | 0.499731 | 8_1:+14908863 | Msa1180700:CDS |
| CTCTTCACTCACCAGAGAAA+GGG | 0.500227 | 8_1:-14908823 | None:intergenic |
| TCAGTAAGAGTTCTCAGAAA+GGG | 0.502446 | 8_1:+14909645 | Msa1180700:CDS |
| GTGTGGGTAATGATAAGTTT+CGG | 0.503235 | 8_1:+14908991 | Msa1180700:CDS |
| GAAATCGTTGGACAAGGAAA+AGG | 0.507749 | 8_1:+14909235 | Msa1180700:CDS |
| CCTTTCTCTGGTGAGTGAAG+AGG | 0.513821 | 8_1:+14908824 | Msa1180700:CDS |
| AATGATTTCGATAATGGAAG+TGG | 0.516836 | 8_1:-14908684 | None:intergenic |
| CGCCATGAAACAAGAGGCGA+AGG | 0.518234 | 8_1:-14908615 | None:intergenic |
| TTCACTTTCATCAACTTCAG+TGG | 0.526000 | 8_1:+14908506 | Msa1180700:CDS |
| GTTGAAGTATCCAGATCTCA+TGG | 0.526419 | 8_1:+14909103 | Msa1180700:CDS |
| TCTGAAGCTTCTGGCAGCGA+TGG | 0.532379 | 8_1:+14909062 | Msa1180700:CDS |
| GGAATGAAGGAGAGTGAGGG+AGG | 0.533569 | 8_1:-14907504 | None:intergenic |
| ATGAAGAAGAGTGGAAAGAT+CGG | 0.534016 | 8_1:-14907525 | None:intergenic |
| ATCGGAATGAAGGAGAGTGA+GGG | 0.539341 | 8_1:-14907507 | None:intergenic |
| GTCAGTAAGAGTTCTCAGAA+AGG | 0.553014 | 8_1:+14909644 | Msa1180700:CDS |
| TGGTGAAAATACTTTGAAGA+AGG | 0.558898 | 8_1:+14909562 | Msa1180700:CDS |
| CATATCTGAAATATGTCGTG+TGG | 0.561959 | 8_1:+14908974 | Msa1180700:CDS |
| ATACAAAGAGGGAAGCGTGC+TGG | 0.568703 | 8_1:+14909542 | Msa1180700:CDS |
| TCACTCACCAGAGAAAGGGC+AGG | 0.573111 | 8_1:-14908819 | None:intergenic |
| AATTTGGTTTGTGGTTGGTG+TGG | 0.575140 | 8_1:-14908720 | None:intergenic |
| GAAGAAGAGTGTAAGCAAAG+AGG | 0.579188 | 8_1:+14907755 | Msa1180700:CDS |
| AGGAGTCATCATATAAAGCA+TGG | 0.581661 | 8_1:-14908907 | None:intergenic |
| AAGCACTGAATCATTATTGG+AGG | 0.583598 | 8_1:-14908763 | None:intergenic |
| ACAAGTCAATAAATTGAATG+TGG | 0.587588 | 8_1:+14909304 | Msa1180700:CDS |
| TTGAAGTATCCAGATCTCAT+GGG | 0.591173 | 8_1:+14909104 | Msa1180700:CDS |
| AGAAACCTGATGAAGAAGAG+TGG | 0.593393 | 8_1:-14907534 | None:intergenic |
| ATTGAAGCGATTCGTTGTTG+CGG | 0.600976 | 8_1:-14907450 | None:intergenic |
| CCTGAAAACACGATACAAAG+AGG | 0.604121 | 8_1:+14909530 | Msa1180700:CDS |
| AGAAGAGTGTAAGCAAAGAG+GGG | 0.609819 | 8_1:+14907757 | Msa1180700:CDS |
| ATGAACTCCAACAGGAAAAG+GGG | 0.611782 | 8_1:-14909021 | None:intergenic |
| GACACCTGATGAGAAATCGT+TGG | 0.616032 | 8_1:+14909223 | Msa1180700:CDS |
| CTGAAAACACGATACAAAGA+GGG | 0.620143 | 8_1:+14909531 | Msa1180700:CDS |
| AACTCCAACAGGAAAAGGGG+TGG | 0.626121 | 8_1:-14909018 | None:intergenic |
| TGATGAGAAATCGTTGGACA+AGG | 0.631692 | 8_1:+14909229 | Msa1180700:CDS |
| TCCAAAGTCAGCAATTGGAA+AGG | 0.633354 | 8_1:+14909139 | Msa1180700:CDS |
| TTAGCACGCCATGAAACAAG+AGG | 0.638632 | 8_1:-14908621 | None:intergenic |
| ATATCTGAAATATGTCGTGT+GGG | 0.639858 | 8_1:+14908975 | Msa1180700:CDS |
| GTGGAAGAGGATATTTCCAA+AGG | 0.646607 | 8_1:+14909323 | Msa1180700:CDS |
| TGAAGTATCCAGATCTCATG+GGG | 0.668354 | 8_1:+14909105 | Msa1180700:CDS |
| TGTTGTCTGAAATGTTGTCG+CGG | 0.705372 | 8_1:+14907808 | Msa1180700:CDS |
| TGTTATCAAATGCAGCCATG+GGG | 0.714680 | 8_1:+14908468 | Msa1180700:intron |
| ATATAGAGAGCACTCCCATG+TGG | 0.723054 | 8_1:+14909501 | Msa1180700:CDS |
| GCTAATTGTACTGTTCAGCG+AGG | 0.740131 | 8_1:+14908639 | Msa1180700:CDS |
| AGTCATCATATAAAGCATGG+AGG | 0.749944 | 8_1:-14908904 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTATTCTATTATAATTACTT+TGG | + | chr8_1:14907614-14907633 | Msa1180700:intron | 10.0% |
| !!! | TGTTATTTGAATTTTTAGAT+TGG | - | chr8_1:14907382-14907401 | None:intergenic | 15.0% |
| !!! | TCCATTTTTTTTTTCATTTT+AGG | + | chr8_1:14907574-14907593 | Msa1180700:intron | 15.0% |
| !!! | CCATTTTTTTTTTCATTTTA+GGG | + | chr8_1:14907575-14907594 | Msa1180700:intron | 15.0% |
| !!! | TAGTTTGATTGTTTAATTTT+AGG | + | chr8_1:14907649-14907668 | Msa1180700:intron | 15.0% |
| !!! | TATTTGAATTTTTAGATTGG+AGG | - | chr8_1:14907379-14907398 | None:intergenic | 20.0% |
| !! | CCCTAAAATGAAAAAAAAAA+TGG | - | chr8_1:14907578-14907597 | None:intergenic | 20.0% |
| !! | AAACAAACTAAAAAATACAG+CGG | - | chr8_1:14907863-14907882 | None:intergenic | 20.0% |
| !!! | AAGGTAAAATAATTTGTGTT+TGG | + | chr8_1:14908040-14908059 | Msa1180700:intron | 20.0% |
| !! | TAGTCATTAAATGTATGAAA+AGG | + | chr8_1:14908130-14908149 | Msa1180700:intron | 20.0% |
| !!! | ATCATCGTTGATTTGTTTTT+GGG | - | chr8_1:14907477-14907496 | None:intergenic | 25.0% |
| !!! | GCTGTATTTTTTAGTTTGTT+TGG | + | chr8_1:14907862-14907881 | Msa1180700:intron | 25.0% |
| !!! | TTTTAGTTTGTTTGGTTTTG+TGG | + | chr8_1:14907870-14907889 | Msa1180700:intron | 25.0% |
| !! | TCATTTTGCAAAACTGTTTT+CGG | + | chr8_1:14907918-14907937 | Msa1180700:intron | 25.0% |
| !! | GTCTTTTGTTAGTAATTTCA+TGG | + | chr8_1:14908208-14908227 | Msa1180700:intron | 25.0% |
| ! | TGACTAACAAAAGAGATATT+CGG | + | chr8_1:14908302-14908321 | Msa1180700:intron | 25.0% |
| ! | CAAAGATTAAACAACAATCT+TGG | - | chr8_1:14908391-14908410 | None:intergenic | 25.0% |
| !! | GAAAGTGAACTTTGTAATTT+TGG | - | chr8_1:14908495-14908514 | None:intergenic | 25.0% |
| ! | GATAACAATGATTTCGATAA+TGG | - | chr8_1:14908693-14908712 | None:intergenic | 25.0% |
| ! | AATAATGATTCAGTGCTTTA+CGG | + | chr8_1:14908768-14908787 | Msa1180700:CDS | 25.0% |
| !!! | CAAAAACTTGTGTTTTGTTA+GGG | + | chr8_1:14909201-14909220 | Msa1180700:CDS | 25.0% |
| ! | ACAAGTCAATAAATTGAATG+TGG | + | chr8_1:14909304-14909323 | Msa1180700:CDS | 25.0% |
| !!! | TTGGTTACAACAAGATTTTT+TGG | - | chr8_1:14909360-14909379 | None:intergenic | 25.0% |
| !! | AAAAAATCTTGTTGTAACCA+AGG | + | chr8_1:14909359-14909378 | Msa1180700:CDS | 25.0% |
| ! | AGCTATAAGGAATATATAAC+AGG | - | chr8_1:14909397-14909416 | None:intergenic | 25.0% |
| !!! | TATATTCCTTATAGCTTGTT+TGG | + | chr8_1:14909401-14909420 | Msa1180700:intron | 25.0% |
| !!! | TTGTGTTTTTTCTTCTTTTC+AGG | + | chr8_1:14909455-14909474 | Msa1180700:intron | 25.0% |
| !! | ATACTTTGAAGAAGGAATTA+TGG | + | chr8_1:14909570-14909589 | Msa1180700:CDS | 25.0% |
| !! | TACTTTGAAGAAGGAATTAT+GGG | + | chr8_1:14909571-14909590 | Msa1180700:CDS | 25.0% |
| ! | GCAAGCAAATAATATCTTAT+TGG | + | chr8_1:14909767-14909786 | Msa1180700:three_prime_UTR | 25.0% |
| !! | TTGAATTTTTAGATTGGAGG+CGG | - | chr8_1:14907376-14907395 | None:intergenic | 30.0% |
| !!! | TCATCGTTGATTTGTTTTTG+GGG | - | chr8_1:14907476-14907495 | None:intergenic | 30.0% |
| !!! | CATCATCGTTGATTTGTTTT+TGG | - | chr8_1:14907478-14907497 | None:intergenic | 30.0% |
| TGGATACATTCTTGTAAAAG+CGG | + | chr8_1:14908060-14908079 | Msa1180700:intron | 30.0% | |
| GATACTAATGTAGTCTCTTT+AGG | - | chr8_1:14908594-14908613 | None:intergenic | 30.0% | |
| !! | AATGATTTCGATAATGGAAG+TGG | - | chr8_1:14908687-14908706 | None:intergenic | 30.0% |
| !! | AATCTAATTTGGTTTGTGGT+TGG | - | chr8_1:14908728-14908747 | None:intergenic | 30.0% |
| !! | GTGAAATCTAATTTGGTTTG+TGG | - | chr8_1:14908732-14908751 | None:intergenic | 30.0% |
| CAACTCAGTGAAATCTAATT+TGG | - | chr8_1:14908739-14908758 | None:intergenic | 30.0% | |
| CAAATTAGATTTCACTGAGT+TGG | + | chr8_1:14908737-14908756 | Msa1180700:CDS | 30.0% | |
| !! | GTAAAGCACTGAATCATTAT+TGG | - | chr8_1:14908769-14908788 | None:intergenic | 30.0% |
| !! | GTGTCTATTTTAGAAAGATC+CGG | - | chr8_1:14908885-14908904 | None:intergenic | 30.0% |
| ACACGACATATTTCAGATAT+GGG | - | chr8_1:14908975-14908994 | None:intergenic | 30.0% | |
| ! | ATATCTGAAATATGTCGTGT+GGG | + | chr8_1:14908975-14908994 | Msa1180700:CDS | 30.0% |
| !! | AACAAAACACAAGTTTTTGG+AGG | - | chr8_1:14909200-14909219 | None:intergenic | 30.0% |
| ! | CCTAACAAAACACAAGTTTT+TGG | - | chr8_1:14909203-14909222 | None:intergenic | 30.0% |
| !! | CCAAAAACTTGTGTTTTGTT+AGG | + | chr8_1:14909200-14909219 | Msa1180700:CDS | 30.0% |
| !! | ATTGACTTGTTGCTTAAGAA+TGG | - | chr8_1:14909294-14909313 | None:intergenic | 30.0% |
| CAATAAATTGAATGTGGAAG+AGG | + | chr8_1:14909310-14909329 | Msa1180700:CDS | 30.0% | |
| TGTTAACCAAACAAGCTATA+AGG | - | chr8_1:14909410-14909429 | None:intergenic | 30.0% | |
| !! | TATAGCTTGTTTGGTTAACA+TGG | + | chr8_1:14909410-14909429 | Msa1180700:intron | 30.0% |
| !! | ATAGCTTGTTTGGTTAACAT+GGG | + | chr8_1:14909411-14909430 | Msa1180700:intron | 30.0% |
| ! | TGGTGAAAATACTTTGAAGA+AGG | + | chr8_1:14909562-14909581 | Msa1180700:CDS | 30.0% |
| ! | GTTTGAAACTTAGACTCAAA+TGG | - | chr8_1:14909623-14909642 | None:intergenic | 30.0% |
| ! | ATTTTCAGCTTATTCATCAC+AGG | - | chr8_1:14909696-14909715 | None:intergenic | 30.0% |
| ATGAAGAAGAGTGGAAAGAT+CGG | - | chr8_1:14907528-14907547 | None:intergenic | 35.0% | |
| ! | AAGAAGAGTGTAAGCAAAGA+GGG | + | chr8_1:14907756-14907775 | Msa1180700:CDS | 35.0% |
| AAAGGTAAAACCCCTAAGTT+TGG | + | chr8_1:14907833-14907852 | Msa1180700:intron | 35.0% | |
| GTTGAACACTAATGTATGCT+TGG | + | chr8_1:14907992-14908011 | Msa1180700:intron | 35.0% | |
| ACTAATGTATGCTTGGTTAG+CGG | + | chr8_1:14907999-14908018 | Msa1180700:intron | 35.0% | |
| GGAGAGGAAAATTGAATTGA+AGG | + | chr8_1:14908021-14908040 | Msa1180700:intron | 35.0% | |
| GGATACATTCTTGTAAAAGC+GGG | + | chr8_1:14908061-14908080 | Msa1180700:intron | 35.0% | |
| GTGTCTTTACTTGATCAGTT+CGG | - | chr8_1:14908235-14908254 | None:intergenic | 35.0% | |
| TTGTTATCAAATGCAGCCAT+GGG | + | chr8_1:14908467-14908486 | Msa1180700:intron | 35.0% | |
| TTCACTTTCATCAACTTCAG+TGG | + | chr8_1:14908506-14908525 | Msa1180700:CDS | 35.0% | |
| !! | AAGCACTGAATCATTATTGG+AGG | - | chr8_1:14908766-14908785 | None:intergenic | 35.0% |
| AGTCATCATATAAAGCATGG+AGG | - | chr8_1:14908907-14908926 | None:intergenic | 35.0% | |
| AGGAGTCATCATATAAAGCA+TGG | - | chr8_1:14908910-14908929 | None:intergenic | 35.0% | |
| ! | TCAAGCAAAGCACAAGATTT+TGG | - | chr8_1:14908951-14908970 | None:intergenic | 35.0% |
| CACACGACATATTTCAGATA+TGG | - | chr8_1:14908976-14908995 | None:intergenic | 35.0% | |
| ! | CATATCTGAAATATGTCGTG+TGG | + | chr8_1:14908974-14908993 | Msa1180700:CDS | 35.0% |
| GTGTGGGTAATGATAAGTTT+CGG | + | chr8_1:14908991-14909010 | Msa1180700:CDS | 35.0% | |
| AATGAACTCCAACAGGAAAA+GGG | - | chr8_1:14909025-14909044 | None:intergenic | 35.0% | |
| TAATGAACTCCAACAGGAAA+AGG | - | chr8_1:14909026-14909045 | None:intergenic | 35.0% | |
| ! | TTGAAGTATCCAGATCTCAT+GGG | + | chr8_1:14909104-14909123 | Msa1180700:CDS | 35.0% |
| !! | TTTGGCTTGATTATGACCTT+TGG | - | chr8_1:14909342-14909361 | None:intergenic | 35.0% |
| !!! | TTCTTTTCAGGTGATTTTGC+TGG | + | chr8_1:14909467-14909486 | Msa1180700:intron | 35.0% |
| CTGAAAACACGATACAAAGA+GGG | + | chr8_1:14909531-14909550 | Msa1180700:CDS | 35.0% | |
| TCAGTAAGAGTTCTCAGAAA+GGG | + | chr8_1:14909645-14909664 | Msa1180700:CDS | 35.0% | |
| ATTGAAGCGATTCGTTGTTG+CGG | - | chr8_1:14907453-14907472 | None:intergenic | 40.0% | |
| TCTTTCCACTCTTCTTCATC+AGG | + | chr8_1:14907529-14907548 | Msa1180700:CDS | 40.0% | |
| AGAAACCTGATGAAGAAGAG+TGG | - | chr8_1:14907537-14907556 | None:intergenic | 40.0% | |
| ! | GAAGAAGAGTGTAAGCAAAG+AGG | + | chr8_1:14907755-14907774 | Msa1180700:CDS | 40.0% |
| ! | AGAAGAGTGTAAGCAAAGAG+GGG | + | chr8_1:14907757-14907776 | Msa1180700:CDS | 40.0% |
| ! | TGTTGTCTGAAATGTTGTCG+CGG | + | chr8_1:14907808-14907827 | Msa1180700:CDS | 40.0% |
| TGAAATGTTGTCGCGGTTAA+AGG | + | chr8_1:14907815-14907834 | Msa1180700:CDS | 40.0% | |
| CTAATGTATGCTTGGTTAGC+GGG | + | chr8_1:14908000-14908019 | Msa1180700:intron | 40.0% | |
| GACAACACTTGACACATTCA+AGG | + | chr8_1:14908364-14908383 | Msa1180700:intron | 40.0% | |
| GTTGTTATCAAATGCAGCCA+TGG | + | chr8_1:14908466-14908485 | Msa1180700:intron | 40.0% | |
| TGTTATCAAATGCAGCCATG+GGG | + | chr8_1:14908468-14908487 | Msa1180700:intron | 40.0% | |
| !! | CTTTGTAATTTTGGTCCCCA+TGG | - | chr8_1:14908486-14908505 | None:intergenic | 40.0% |
| !! | AATTTGGTTTGTGGTTGGTG+TGG | - | chr8_1:14908723-14908742 | None:intergenic | 40.0% |
| ! | GCATGAATTGATTTCTTCGC+CGG | + | chr8_1:14908863-14908882 | Msa1180700:CDS | 40.0% |
| ! | AGCAAAGCACAAGATTTTGG+AGG | - | chr8_1:14908948-14908967 | None:intergenic | 40.0% |
| ATGAACTCCAACAGGAAAAG+GGG | - | chr8_1:14909024-14909043 | None:intergenic | 40.0% | |
| TCACTGTAATGAACTCCAAC+AGG | - | chr8_1:14909032-14909051 | None:intergenic | 40.0% | |
| !! | TCAGAAGATTCTGAAGCTTC+TGG | + | chr8_1:14909053-14909072 | Msa1180700:CDS | 40.0% |
| ! | GTTGAAGTATCCAGATCTCA+TGG | + | chr8_1:14909103-14909122 | Msa1180700:CDS | 40.0% |
| ! | TGAAGTATCCAGATCTCATG+GGG | + | chr8_1:14909105-14909124 | Msa1180700:CDS | 40.0% |
| TGTGTTCCAAAGTCAGCAAT+TGG | + | chr8_1:14909134-14909153 | Msa1180700:CDS | 40.0% | |
| TCCAAAGTCAGCAATTGGAA+AGG | + | chr8_1:14909139-14909158 | Msa1180700:CDS | 40.0% | |
| !! | TCCTTTCCAATTGCTGACTT+TGG | - | chr8_1:14909143-14909162 | None:intergenic | 40.0% |
| ! | ATGTTGAAGCATCACCTGTT+TGG | + | chr8_1:14909165-14909184 | Msa1180700:CDS | 40.0% |
| !! | AACACAAGTTTTTGGAGGTG+AGG | - | chr8_1:14909195-14909214 | None:intergenic | 40.0% |
| TTGTCCAACGATTTCTCATC+AGG | - | chr8_1:14909230-14909249 | None:intergenic | 40.0% | |
| TGATGAGAAATCGTTGGACA+AGG | + | chr8_1:14909229-14909248 | Msa1180700:CDS | 40.0% | |
| GAAATCGTTGGACAAGGAAA+AGG | + | chr8_1:14909235-14909254 | Msa1180700:CDS | 40.0% | |
| GTGGAAGAGGATATTTCCAA+AGG | + | chr8_1:14909323-14909342 | Msa1180700:CDS | 40.0% | |
| TTGTTGTAACCAAGGTAGTG+TGG | + | chr8_1:14909367-14909386 | Msa1180700:intron | 40.0% | |
| !! | TTTTCAGGATTCAGCCACAT+GGG | - | chr8_1:14909518-14909537 | None:intergenic | 40.0% |
| ! | CCTCTTTGTATCGTGTTTTC+AGG | - | chr8_1:14909533-14909552 | None:intergenic | 40.0% |
| CCTGAAAACACGATACAAAG+AGG | + | chr8_1:14909530-14909549 | Msa1180700:CDS | 40.0% | |
| GTCAGTAAGAGTTCTCAGAA+AGG | + | chr8_1:14909644-14909663 | Msa1180700:CDS | 40.0% | |
| GAAAGGGTTTCTTGACCTAT+TGG | + | chr8_1:14909661-14909680 | Msa1180700:CDS | 40.0% | |
| ATCGGAATGAAGGAGAGTGA+GGG | - | chr8_1:14907510-14907529 | None:intergenic | 45.0% | |
| GAGTGGAAAGATCGGAATGA+AGG | - | chr8_1:14907520-14907539 | None:intergenic | 45.0% | |
| ACAGCGGAGAACCAAACTTA+GGG | - | chr8_1:14907847-14907866 | None:intergenic | 45.0% | |
| TACAGCGGAGAACCAAACTT+AGG | - | chr8_1:14907848-14907867 | None:intergenic | 45.0% | |
| TTAGCACGCCATGAAACAAG+AGG | - | chr8_1:14908624-14908643 | None:intergenic | 45.0% | |
| GCTAATTGTACTGTTCAGCG+AGG | + | chr8_1:14908639-14908658 | Msa1180700:CDS | 45.0% | |
| ! | AGAGAAAGGGCAGGCTTTAT+TGG | - | chr8_1:14908813-14908832 | None:intergenic | 45.0% |
| AATAAAGCCTGCCCTTTCTC+TGG | + | chr8_1:14908812-14908831 | Msa1180700:CDS | 45.0% | |
| CTCTTCACTCACCAGAGAAA+GGG | - | chr8_1:14908826-14908845 | None:intergenic | 45.0% | |
| !! | GGAGGTGACATTTTCAAGCA+AGG | - | chr8_1:14908930-14908949 | None:intergenic | 45.0% |
| CAATGTTTCCCCATGAGATC+TGG | - | chr8_1:14909116-14909135 | None:intergenic | 45.0% | |
| GACACCTGATGAGAAATCGT+TGG | + | chr8_1:14909223-14909242 | Msa1180700:CDS | 45.0% | |
| !! | ATATAGAGAGCACTCCCATG+TGG | + | chr8_1:14909501-14909520 | Msa1180700:CDS | 45.0% |
| !! | GTTTTCAGGATTCAGCCACA+TGG | - | chr8_1:14909519-14909538 | None:intergenic | 45.0% |
| CACAGGAAGCTTCTTCCAAT+AGG | - | chr8_1:14909679-14909698 | None:intergenic | 45.0% | |
| GATCGGAATGAAGGAGAGTG+AGG | - | chr8_1:14907511-14907530 | None:intergenic | 50.0% | |
| CAGCGGAGAACCAAACTTAG+GGG | - | chr8_1:14907846-14907865 | None:intergenic | 50.0% | |
| ! | CTCCTTCGCCTCTTGTTTCA+TGG | + | chr8_1:14908613-14908632 | Msa1180700:CDS | 50.0% |
| ! | GGTTTGTGGTTGGTGTGGTT+TGG | - | chr8_1:14908718-14908737 | None:intergenic | 50.0% |
| !! | AGGGCAGGCTTTATTGGAGT+TGG | - | chr8_1:14908807-14908826 | None:intergenic | 50.0% |
| CCTCTTCACTCACCAGAGAA+AGG | - | chr8_1:14908827-14908846 | None:intergenic | 50.0% | |
| CCTTTCTCTGGTGAGTGAAG+AGG | + | chr8_1:14908824-14908843 | Msa1180700:CDS | 50.0% | |
| ! | AAGTCCACCCCTTTTCCTGT+TGG | + | chr8_1:14909014-14909033 | Msa1180700:CDS | 50.0% |
| AACTCCAACAGGAAAAGGGG+TGG | - | chr8_1:14909021-14909040 | None:intergenic | 50.0% | |
| ACAGGTGAACCACACTACCT+TGG | - | chr8_1:14909379-14909398 | None:intergenic | 50.0% | |
| ! | ATACAAAGAGGGAAGCGTGC+TGG | + | chr8_1:14909542-14909561 | Msa1180700:CDS | 50.0% |
| GGAATGAAGGAGAGTGAGGG+AGG | - | chr8_1:14907507-14907526 | None:intergenic | 55.0% | |
| GTATGCTTGGTTAGCGGGAG+AGG | + | chr8_1:14908005-14908024 | Msa1180700:intron | 55.0% | |
| CGCCATGAAACAAGAGGCGA+AGG | - | chr8_1:14908618-14908637 | None:intergenic | 55.0% | |
| TCACTCACCAGAGAAAGGGC+AGG | - | chr8_1:14908822-14908841 | None:intergenic | 55.0% | |
| ! | TCTGAAGCTTCTGGCAGCGA+TGG | + | chr8_1:14909062-14909081 | Msa1180700:CDS | 55.0% |
| GGAGGTGAGGTAAGCCAAAC+AGG | - | chr8_1:14909182-14909201 | None:intergenic | 55.0% | |
| CGATGGTGTGACGCAGGGTT+TGG | + | chr8_1:14909079-14909098 | Msa1180700:CDS | 60.0% | |
| TGGCAGCGATGGTGTGACGC+AGG | + | chr8_1:14909073-14909092 | Msa1180700:CDS | 65.0% | |
| GGCAGCGATGGTGTGACGCA+GGG | + | chr8_1:14909074-14909093 | Msa1180700:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8_1 | gene | 14907373 | 14909850 | 14907373 | ID=Msa1180700;Name=Msa1180700 |
| chr8_1 | mRNA | 14907373 | 14909850 | 14907373 | ID=Msa1180700-mRNA-1;Parent=Msa1180700;Name=Msa1180700-mRNA-1;_AED=0.04;_eAED=0.04;_QI=58|1|1|1|1|1|4|144|453 |
| chr8_1 | exon | 14907373 | 14907550 | 14907373 | ID=Msa1180700-mRNA-1:exon:5545;Parent=Msa1180700-mRNA-1 |
| chr8_1 | exon | 14907723 | 14907836 | 14907723 | ID=Msa1180700-mRNA-1:exon:5546;Parent=Msa1180700-mRNA-1 |
| chr8_1 | exon | 14908483 | 14909380 | 14908483 | ID=Msa1180700-mRNA-1:exon:5547;Parent=Msa1180700-mRNA-1 |
| chr8_1 | exon | 14909477 | 14909850 | 14909477 | ID=Msa1180700-mRNA-1:exon:5548;Parent=Msa1180700-mRNA-1 |
| chr8_1 | five_prime_UTR | 14907373 | 14907430 | 14907373 | ID=Msa1180700-mRNA-1:five_prime_utr;Parent=Msa1180700-mRNA-1 |
| chr8_1 | CDS | 14907431 | 14907550 | 14907431 | ID=Msa1180700-mRNA-1:cds;Parent=Msa1180700-mRNA-1 |
| chr8_1 | CDS | 14907723 | 14907836 | 14907723 | ID=Msa1180700-mRNA-1:cds;Parent=Msa1180700-mRNA-1 |
| chr8_1 | CDS | 14908483 | 14909380 | 14908483 | ID=Msa1180700-mRNA-1:cds;Parent=Msa1180700-mRNA-1 |
| chr8_1 | CDS | 14909477 | 14909706 | 14909477 | ID=Msa1180700-mRNA-1:cds;Parent=Msa1180700-mRNA-1 |
| chr8_1 | three_prime_UTR | 14909707 | 14909850 | 14909707 | ID=Msa1180700-mRNA-1:three_prime_utr;Parent=Msa1180700-mRNA-1 |
| Gene Sequence |
| Protein sequence |
