Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene067002.t1 | XP_013466249.1 | 97.7 | 130 | 3 | 0 | 1 | 130 | 1 | 130 | 2.00E-61 | 245 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene067002.t1 | Q9ZT05 | 97.6 | 127 | 3 | 0 | 1 | 127 | 1 | 127 | 3.1e-63 | 242.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene067002.t1 | I3SQF1 | 97.7 | 130 | 3 | 0 | 1 | 130 | 1 | 130 | 1.4e-61 | 245.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049173 | MS.gene067002 | 0.813381 | 2.63E-51 | -1.69E-46 |
| MS.gene049320 | MS.gene067002 | 0.801462 | 8.90E-49 | -1.69E-46 |
| MS.gene049356 | MS.gene067002 | 0.818346 | 2.06E-52 | -1.69E-46 |
| MS.gene049369 | MS.gene067002 | 0.834875 | 2.33E-56 | -1.69E-46 |
| MS.gene049427 | MS.gene067002 | 0.816894 | 4.37E-52 | -1.69E-46 |
| MS.gene049491 | MS.gene067002 | 0.822884 | 1.87E-53 | -1.69E-46 |
| MS.gene049609 | MS.gene067002 | 0.83942 | 1.60E-57 | -1.69E-46 |
| MS.gene049614 | MS.gene067002 | 0.807975 | 3.89E-50 | -1.69E-46 |
| MS.gene049661 | MS.gene067002 | 0.862897 | 3.66E-64 | -1.69E-46 |
| MS.gene049662 | MS.gene067002 | 0.858944 | 5.82E-63 | -1.69E-46 |
| MS.gene049758 | MS.gene067002 | 0.802488 | 5.48E-49 | -1.69E-46 |
| MS.gene049805 | MS.gene067002 | 0.810477 | 1.13E-50 | -1.69E-46 |
| MS.gene050076 | MS.gene067002 | 0.861809 | 7.90E-64 | -1.69E-46 |
| MS.gene050301 | MS.gene067002 | 0.823491 | 1.35E-53 | -1.69E-46 |
| MS.gene050397 | MS.gene067002 | 0.847005 | 1.52E-59 | -1.69E-46 |
| MS.gene050404 | MS.gene067002 | 0.810786 | 9.70E-51 | -1.69E-46 |
| MS.gene050432 | MS.gene067002 | 0.890871 | 6.66E-74 | -1.69E-46 |
| MS.gene050529 | MS.gene067002 | 0.808591 | 2.87E-50 | -1.69E-46 |
| MS.gene050593 | MS.gene067002 | 0.824053 | 9.94E-54 | -1.69E-46 |
| MS.gene050670 | MS.gene067002 | 0.810715 | 1.00E-50 | -1.69E-46 |
| MS.gene050686 | MS.gene067002 | 0.808746 | 2.66E-50 | -1.69E-46 |
| MS.gene050767 | MS.gene067002 | 0.890896 | 6.51E-74 | -1.69E-46 |
| MS.gene050770 | MS.gene067002 | 0.893753 | 4.69E-75 | -1.69E-46 |
| MS.gene050772 | MS.gene067002 | 0.850498 | 1.63E-60 | -1.69E-46 |
| MS.gene05101 | MS.gene067002 | 0.859301 | 4.55E-63 | -1.69E-46 |
| MS.gene05105 | MS.gene067002 | 0.878951 | 1.85E-69 | -1.69E-46 |
| MS.gene051228 | MS.gene067002 | 0.869214 | 3.66E-66 | -1.69E-46 |
| MS.gene051666 | MS.gene067002 | 0.822799 | 1.95E-53 | -1.69E-46 |
| MS.gene051692 | MS.gene067002 | 0.833794 | 4.35E-56 | -1.69E-46 |
| MS.gene051810 | MS.gene067002 | 0.836067 | 1.16E-56 | -1.69E-46 |
| MS.gene051858 | MS.gene067002 | 0.823608 | 1.26E-53 | -1.69E-46 |
| MS.gene051877 | MS.gene067002 | 0.810305 | 1.23E-50 | -1.69E-46 |
| MS.gene052078 | MS.gene067002 | 0.843335 | 1.49E-58 | -1.69E-46 |
| MS.gene052188 | MS.gene067002 | 0.817099 | 3.93E-52 | -1.69E-46 |
| MS.gene052271 | MS.gene067002 | 0.847868 | 8.81E-60 | -1.69E-46 |
| MS.gene052287 | MS.gene067002 | 0.80206 | 6.71E-49 | -1.69E-46 |
| MS.gene052290 | MS.gene067002 | 0.860833 | 1.57E-63 | -1.69E-46 |
| MS.gene052293 | MS.gene067002 | 0.805962 | 1.04E-49 | -1.69E-46 |
| MS.gene052310 | MS.gene067002 | 0.850312 | 1.84E-60 | -1.69E-46 |
| MS.gene052322 | MS.gene067002 | 0.805724 | 1.16E-49 | -1.69E-46 |
| MS.gene052326 | MS.gene067002 | 0.811666 | 6.25E-51 | -1.69E-46 |
| MS.gene052340 | MS.gene067002 | 0.833894 | 4.10E-56 | -1.69E-46 |
| MS.gene052379 | MS.gene067002 | 0.827094 | 1.89E-54 | -1.69E-46 |
| MS.gene052421 | MS.gene067002 | 0.813089 | 3.05E-51 | -1.69E-46 |
| MS.gene052481 | MS.gene067002 | 0.800039 | 1.74E-48 | -1.69E-46 |
| MS.gene052484 | MS.gene067002 | 0.834941 | 2.24E-56 | -1.69E-46 |
| MS.gene052485 | MS.gene067002 | 0.804901 | 1.73E-49 | -1.69E-46 |
| MS.gene052508 | MS.gene067002 | 0.830539 | 2.77E-55 | -1.69E-46 |
| MS.gene052510 | MS.gene067002 | 0.817966 | 2.51E-52 | -1.69E-46 |
| MS.gene053009 | MS.gene067002 | 0.855366 | 6.62E-62 | -1.69E-46 |
| MS.gene053103 | MS.gene067002 | 0.843997 | 9.93E-59 | -1.69E-46 |
| MS.gene053104 | MS.gene067002 | 0.866781 | 2.22E-65 | -1.69E-46 |
| MS.gene053207 | MS.gene067002 | 0.83534 | 1.78E-56 | -1.69E-46 |
| MS.gene053209 | MS.gene067002 | 0.909648 | 4.51E-82 | -1.69E-46 |
| MS.gene053213 | MS.gene067002 | 0.819621 | 1.06E-52 | -1.69E-46 |
| MS.gene053286 | MS.gene067002 | 0.807715 | 4.41E-50 | -1.69E-46 |
| MS.gene053322 | MS.gene067002 | 0.852078 | 5.84E-61 | -1.69E-46 |
| MS.gene053384 | MS.gene067002 | 0.823351 | 1.45E-53 | -1.69E-46 |
| MS.gene053449 | MS.gene067002 | 0.832116 | 1.14E-55 | -1.69E-46 |
| MS.gene053557 | MS.gene067002 | 0.810739 | 9.93E-51 | -1.69E-46 |
| MS.gene053574 | MS.gene067002 | 0.814318 | 1.64E-51 | -1.69E-46 |
| MS.gene053632 | MS.gene067002 | 0.804531 | 2.07E-49 | -1.69E-46 |
| MS.gene053813 | MS.gene067002 | 0.811064 | 8.44E-51 | -1.69E-46 |
| MS.gene053916 | MS.gene067002 | 0.808428 | 3.11E-50 | -1.69E-46 |
| MS.gene054376 | MS.gene067002 | 0.893228 | 7.64E-75 | -1.69E-46 |
| MS.gene054600 | MS.gene067002 | 0.809843 | 1.55E-50 | -1.69E-46 |
| MS.gene054642 | MS.gene067002 | 0.823815 | 1.13E-53 | -1.69E-46 |
| MS.gene054802 | MS.gene067002 | 0.821003 | 5.09E-53 | -1.69E-46 |
| MS.gene054803 | MS.gene067002 | 0.820669 | 6.07E-53 | -1.69E-46 |
| MS.gene054804 | MS.gene067002 | 0.826221 | 3.06E-54 | -1.69E-46 |
| MS.gene05505 | MS.gene067002 | 0.843883 | 1.07E-58 | -1.69E-46 |
| MS.gene055116 | MS.gene067002 | 0.87797 | 4.10E-69 | -1.69E-46 |
| MS.gene055206 | MS.gene067002 | 0.80004 | 1.74E-48 | -1.69E-46 |
| MS.gene055212 | MS.gene067002 | 0.823104 | 1.66E-53 | -1.69E-46 |
| MS.gene055254 | MS.gene067002 | 0.901854 | 1.76E-78 | -1.69E-46 |
| MS.gene055277 | MS.gene067002 | 0.859593 | 3.72E-63 | -1.69E-46 |
| MS.gene055289 | MS.gene067002 | 0.806004 | 1.02E-49 | -1.69E-46 |
| MS.gene055397 | MS.gene067002 | 0.854431 | 1.24E-61 | -1.69E-46 |
| MS.gene055422 | MS.gene067002 | 0.804378 | 2.22E-49 | -1.69E-46 |
| MS.gene055499 | MS.gene067002 | 0.865327 | 6.40E-65 | -1.69E-46 |
| MS.gene055636 | MS.gene067002 | 0.806449 | 8.18E-50 | -1.69E-46 |
| MS.gene055647 | MS.gene067002 | 0.835922 | 1.27E-56 | -1.69E-46 |
| MS.gene055722 | MS.gene067002 | 0.878071 | 3.78E-69 | -1.69E-46 |
| MS.gene05592 | MS.gene067002 | 0.897717 | 1.07E-76 | -1.69E-46 |
| MS.gene056083 | MS.gene067002 | 0.803298 | 3.73E-49 | -1.69E-46 |
| MS.gene056191 | MS.gene067002 | 0.84881 | 4.84E-60 | -1.69E-46 |
| MS.gene056374 | MS.gene067002 | 0.819559 | 1.09E-52 | -1.69E-46 |
| MS.gene056407 | MS.gene067002 | 0.814546 | 1.46E-51 | -1.69E-46 |
| MS.gene056410 | MS.gene067002 | 0.863248 | 2.85E-64 | -1.69E-46 |
| MS.gene056511 | MS.gene067002 | 0.820241 | 7.61E-53 | -1.69E-46 |
| MS.gene056609 | MS.gene067002 | 0.888914 | 3.87E-73 | -1.69E-46 |
| MS.gene056612 | MS.gene067002 | 0.800928 | 1.15E-48 | -1.69E-46 |
| MS.gene056657 | MS.gene067002 | 0.803546 | 3.31E-49 | -1.69E-46 |
| MS.gene056897 | MS.gene067002 | 0.835206 | 1.92E-56 | -1.69E-46 |
| MS.gene05702 | MS.gene067002 | 0.822901 | 1.85E-53 | -1.69E-46 |
| MS.gene057076 | MS.gene067002 | 0.810584 | 1.07E-50 | -1.69E-46 |
| MS.gene057123 | MS.gene067002 | 0.803458 | 3.45E-49 | -1.69E-46 |
| MS.gene057129 | MS.gene067002 | 0.877088 | 8.32E-69 | -1.69E-46 |
| MS.gene057146 | MS.gene067002 | 0.803241 | 3.83E-49 | -1.69E-46 |
| MS.gene057295 | MS.gene067002 | 0.844579 | 6.93E-59 | -1.69E-46 |
| MS.gene057331 | MS.gene067002 | 0.870394 | 1.51E-66 | -1.69E-46 |
| MS.gene05733 | MS.gene067002 | 0.8107 | 1.01E-50 | -1.69E-46 |
| MS.gene05735 | MS.gene067002 | 0.826339 | 2.86E-54 | -1.69E-46 |
| MS.gene057416 | MS.gene067002 | 0.832689 | 8.19E-56 | -1.69E-46 |
| MS.gene057450 | MS.gene067002 | 0.811227 | 7.78E-51 | -1.69E-46 |
| MS.gene057796 | MS.gene067002 | 0.818801 | 1.62E-52 | -1.69E-46 |
| MS.gene057926 | MS.gene067002 | 0.830424 | 2.96E-55 | -1.69E-46 |
| MS.gene058006 | MS.gene067002 | 0.809723 | 1.64E-50 | -1.69E-46 |
| MS.gene058019 | MS.gene067002 | 0.824895 | 6.30E-54 | -1.69E-46 |
| MS.gene05806 | MS.gene067002 | 0.814526 | 1.47E-51 | -1.69E-46 |
| MS.gene058082 | MS.gene067002 | 0.860093 | 2.63E-63 | -1.69E-46 |
| MS.gene058293 | MS.gene067002 | 0.846013 | 2.84E-59 | -1.69E-46 |
| MS.gene058323 | MS.gene067002 | 0.818007 | 2.45E-52 | -1.69E-46 |
| MS.gene058365 | MS.gene067002 | 0.813945 | 1.98E-51 | -1.69E-46 |
| MS.gene058419 | MS.gene067002 | 0.804535 | 2.06E-49 | -1.69E-46 |
| MS.gene058459 | MS.gene067002 | 0.819001 | 1.46E-52 | -1.69E-46 |
| MS.gene058660 | MS.gene067002 | 0.849768 | 2.61E-60 | -1.69E-46 |
| MS.gene058723 | MS.gene067002 | 0.860534 | 1.93E-63 | -1.69E-46 |
| MS.gene058748 | MS.gene067002 | 0.832319 | 1.01E-55 | -1.69E-46 |
| MS.gene058787 | MS.gene067002 | 0.833502 | 5.14E-56 | -1.69E-46 |
| MS.gene058928 | MS.gene067002 | 0.815896 | 7.31E-52 | -1.69E-46 |
| MS.gene059029 | MS.gene067002 | 0.826902 | 2.10E-54 | -1.69E-46 |
| MS.gene05907 | MS.gene067002 | 0.804231 | 2.39E-49 | -1.69E-46 |
| MS.gene05913 | MS.gene067002 | 0.81801 | 2.45E-52 | -1.69E-46 |
| MS.gene059326 | MS.gene067002 | 0.82456 | 7.55E-54 | -1.69E-46 |
| MS.gene059366 | MS.gene067002 | 0.803453 | 3.46E-49 | -1.69E-46 |
| MS.gene059518 | MS.gene067002 | 0.800158 | 1.64E-48 | -1.69E-46 |
| MS.gene059536 | MS.gene067002 | 0.839782 | 1.29E-57 | -1.69E-46 |
| MS.gene059597 | MS.gene067002 | 0.819494 | 1.13E-52 | -1.69E-46 |
| MS.gene059676 | MS.gene067002 | 0.835438 | 1.68E-56 | -1.69E-46 |
| MS.gene059688 | MS.gene067002 | 0.879838 | 8.99E-70 | -1.69E-46 |
| MS.gene059692 | MS.gene067002 | 0.900875 | 4.73E-78 | -1.69E-46 |
| MS.gene059695 | MS.gene067002 | 0.895797 | 6.81E-76 | -1.69E-46 |
| MS.gene059711 | MS.gene067002 | 0.841633 | 4.22E-58 | -1.69E-46 |
| MS.gene059858 | MS.gene067002 | 0.820829 | 5.58E-53 | -1.69E-46 |
| MS.gene059894 | MS.gene067002 | 0.828563 | 8.39E-55 | -1.69E-46 |
| MS.gene059902 | MS.gene067002 | 0.819715 | 1.00E-52 | -1.69E-46 |
| MS.gene059960 | MS.gene067002 | 0.845471 | 3.98E-59 | -1.69E-46 |
| MS.gene059974 | MS.gene067002 | 0.852985 | 3.22E-61 | -1.69E-46 |
| MS.gene060005 | MS.gene067002 | 0.818131 | 2.30E-52 | -1.69E-46 |
| MS.gene060356 | MS.gene067002 | 0.800497 | 1.40E-48 | -1.69E-46 |
| MS.gene06037 | MS.gene067002 | 0.867404 | 1.40E-65 | -1.69E-46 |
| MS.gene060403 | MS.gene067002 | 0.818932 | 1.51E-52 | -1.69E-46 |
| MS.gene060804 | MS.gene067002 | 0.888949 | 3.75E-73 | -1.69E-46 |
| MS.gene060818 | MS.gene067002 | 0.852163 | 5.53E-61 | -1.69E-46 |
| MS.gene060835 | MS.gene067002 | 0.818329 | 2.07E-52 | -1.69E-46 |
| MS.gene060837 | MS.gene067002 | 0.832418 | 9.56E-56 | -1.69E-46 |
| MS.gene061041 | MS.gene067002 | 0.867393 | 1.41E-65 | -1.69E-46 |
| MS.gene061049 | MS.gene067002 | 0.842382 | 2.68E-58 | -1.69E-46 |
| MS.gene061051 | MS.gene067002 | -0.85141 | 9.04E-61 | -1.69E-46 |
| MS.gene061200 | MS.gene067002 | 0.858555 | 7.60E-63 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene067002.t1 | MTR_1g026820 | 97.692 | 130 | 3 | 0 | 1 | 130 | 1 | 130 | 5.83e-88 | 251 |
| MS.gene067002.t1 | MTR_1g026820 | 98.980 | 98 | 1 | 0 | 33 | 130 | 1 | 98 | 5.12e-65 | 192 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene067002.t1 | AT1G30380 | 82.443 | 131 | 21 | 2 | 1 | 130 | 1 | 130 | 2.16e-73 | 215 |
Find 50 sgRNAs with CRISPR-Local
Find 66 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATTGGTGTTGGTGTTGTTCT+TGG | 0.264103 | 1.3:-12760850 | MS.gene067002:CDS |
| GCGCAAGGGCAAGGGTGCTT+TGG | 0.287491 | 1.3:-12761361 | MS.gene067002:CDS |
| ATCTTCCAGCAAACAACATT+AGG | 0.298751 | 1.3:+12761010 | None:intergenic |
| TTGCCCTTGCGCCTTGTCAT+AGG | 0.312155 | 1.3:+12761372 | None:intergenic |
| CGCAAGGGCAAGGGTGCTTT+GGG | 0.326430 | 1.3:-12761360 | MS.gene067002:CDS |
| GTTCACCCTTGCTGATACTT+TGG | 0.359682 | 1.3:-12760896 | MS.gene067002:CDS |
| TTGCAGACTGGTGACCCAGC+TGG | 0.370604 | 1.3:-12760919 | MS.gene067002:CDS |
| ACCTTCTCCTATCCAGGGTT+TGG | 0.377236 | 1.3:-12761638 | MS.gene067002:intron |
| ATGTTGTTTGCTGGAAGATT+TGG | 0.379579 | 1.3:-12761006 | MS.gene067002:CDS |
| TTGAAATCATACCACATTAG+TGG | 0.382365 | 1.3:+12761311 | None:intergenic |
| GGAGTTCGTTGTGACTTCAT+TGG | 0.392047 | 1.3:-12761339 | MS.gene067002:CDS |
| TCTTCCAGCAAACAACATTA+GGG | 0.393888 | 1.3:+12761011 | None:intergenic |
| ACAACCCTAATGTTGTTTGC+TGG | 0.398888 | 1.3:-12761015 | MS.gene067002:CDS |
| CTTGCGCCTTGTCATAGGTT+GGG | 0.405272 | 1.3:+12761377 | None:intergenic |
| CCTTGCGCCTTGTCATAGGT+TGG | 0.408508 | 1.3:+12761376 | None:intergenic |
| TGCAGACTGGTGACCCAGCT+GGG | 0.430536 | 1.3:-12760918 | MS.gene067002:CDS |
| ACTCAAACCTTCTCCTATCC+AGG | 0.437202 | 1.3:-12761644 | MS.gene067002:CDS |
| TCAGCAAGGGTGAACCCAGC+TGG | 0.438597 | 1.3:+12760904 | None:intergenic |
| GATTGGCTCCCTCAGCAAAC+AGG | 0.449260 | 1.3:-12760984 | MS.gene067002:CDS |
| CTTGCTGATACTTTGGCTTG+TGG | 0.450020 | 1.3:-12760889 | MS.gene067002:CDS |
| ACAACTTTGCCTCAATTCAC+TGG | 0.462189 | 1.3:-12761679 | MS.gene067002:CDS |
| GGAACTGTTGGACACATCAT+TGG | 0.485809 | 1.3:-12760868 | MS.gene067002:CDS |
| ACTTTGGCTTGTGGAACTGT+TGG | 0.485908 | 1.3:-12760880 | MS.gene067002:CDS |
| AGCAACAGCAGGACTAAAGC+TGG | 0.487183 | 1.3:-12760959 | MS.gene067002:CDS |
| GTTGCTTTCCTGTTTGCTGA+GGG | 0.491197 | 1.3:+12760976 | None:intergenic |
| TATGACAAGGCGCAAGGGCA+AGG | 0.493251 | 1.3:-12761370 | MS.gene067002:CDS |
| GTTTGCTGGAAGATTTGGAT+TGG | 0.494741 | 1.3:-12761001 | MS.gene067002:CDS |
| GTTCTTGGTCTTAAGAACAT+TGG | 0.536798 | 1.3:-12760835 | MS.gene067002:CDS |
| AGGGACTCAGGCTTGCAGAC+TGG | 0.536809 | 1.3:-12760931 | MS.gene067002:CDS |
| CAGGACTAAAGCTGGAGACA+AGG | 0.539368 | 1.3:-12760951 | MS.gene067002:CDS |
| ATGACAAGGCGCAAGGGCAA+GGG | 0.543094 | 1.3:-12761369 | MS.gene067002:CDS |
| TGTTGCTTTCCTGTTTGCTG+AGG | 0.553786 | 1.3:+12760975 | None:intergenic |
| ACCAAACCCTGGATAGGAGA+AGG | 0.553964 | 1.3:+12761637 | None:intergenic |
| CCAACCTATGACAAGGCGCA+AGG | 0.557141 | 1.3:-12761376 | MS.gene067002:CDS |
| AAGCTGGAGACAAGGGACTC+AGG | 0.559360 | 1.3:-12760943 | MS.gene067002:CDS |
| CAGCAAGGGTGAACCCAGCT+GGG | 0.567280 | 1.3:+12760905 | None:intergenic |
| ATAGGAGAAGGTTTGAGTTG+TGG | 0.576762 | 1.3:+12761649 | None:intergenic |
| TTAATTACCAAACCCTGGAT+AGG | 0.584959 | 1.3:+12761631 | None:intergenic |
| CATTGGTTCATCCACTAATG+TGG | 0.586749 | 1.3:-12761322 | MS.gene067002:intron |
| CAACCTATGACAAGGCGCAA+GGG | 0.587502 | 1.3:-12761375 | MS.gene067002:CDS |
| AGGACTAAAGCTGGAGACAA+GGG | 0.595838 | 1.3:-12760950 | MS.gene067002:CDS |
| CTCAAACCTTCTCCTATCCA+GGG | 0.595941 | 1.3:-12761643 | MS.gene067002:CDS |
| CACAAGCCAAAGTATCAGCA+AGG | 0.599093 | 1.3:+12760890 | None:intergenic |
| AGTGGTTAATTACCAAACCC+TGG | 0.602633 | 1.3:+12761626 | None:intergenic |
| GGTCTAAGACCAGTGAATTG+AGG | 0.606924 | 1.3:+12761670 | None:intergenic |
| GCAAACAGGAAAGCAACAGC+AGG | 0.611764 | 1.3:-12760970 | MS.gene067002:CDS |
| GTTGGACACATCATTGGTGT+TGG | 0.612231 | 1.3:-12760862 | MS.gene067002:CDS |
| ACAAGCCAAAGTATCAGCAA+GGG | 0.663311 | 1.3:+12760891 | None:intergenic |
| TTGCTGCCCAACCTATGACA+AGG | 0.674324 | 1.3:-12761383 | MS.gene067002:CDS |
| ACATGTTGGACAGATTATGG+TGG | 0.710941 | 1.3:-12761043 | MS.gene067002:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TACTGTATATATTTAAATTT+TGG | - | chr1.3:12761069-12761088 | MS.gene067002:intron | 10.0% |
| !!! | TTTAAGTTTTATAATTCATA+AGG | + | chr1.3:12761030-12761049 | None:intergenic | 10.0% |
| !! | TTTAAATATATACAGTATGA+AGG | + | chr1.3:12761066-12761085 | None:intergenic | 15.0% |
| !!! | ATTCATTTTTGTAAATTGTA+TGG | + | chr1.3:12760998-12761017 | None:intergenic | 15.0% |
| !! | AAAACTATACATATCATGAT+TGG | + | chr1.3:12761392-12761411 | None:intergenic | 20.0% |
| !! | ATGAATAAAAACATTGGAAT+TGG | + | chr1.3:12761246-12761265 | None:intergenic | 20.0% |
| !! | TATTCTATATACTTACTTCT+TGG | - | chr1.3:12760971-12760990 | MS.gene067002:CDS | 20.0% |
| !!! | TGTATATGTTTTTTATGTGA+AGG | - | chr1.3:12761116-12761135 | MS.gene067002:intron | 20.0% |
| AAATGATGACATGAAAAGAG+TGG | + | chr1.3:12760917-12760936 | None:intergenic | 30.0% | |
| AAGTTACAGATTCTAACAAG+TGG | + | chr1.3:12761444-12761463 | None:intergenic | 30.0% | |
| CGCACTATGAATAAAAACAT+TGG | + | chr1.3:12761252-12761271 | None:intergenic | 30.0% | |
| TTGAAATCATACCACATTAG+TGG | + | chr1.3:12761214-12761233 | None:intergenic | 30.0% | |
| TTTGCAAGTTTCGCAATAAT+TGG | + | chr1.3:12761420-12761439 | None:intergenic | 30.0% | |
| ! | TATGATTGACTTTGACATGT+TGG | - | chr1.3:12761465-12761484 | MS.gene067002:intron | 30.0% |
| ATCTTCCAGCAAACAACATT+AGG | + | chr1.3:12761515-12761534 | None:intergenic | 35.0% | |
| TCTTCCAGCAAACAACATTA+GGG | + | chr1.3:12761514-12761533 | None:intergenic | 35.0% | |
| TTAATTACCAAACCCTGGAT+AGG | + | chr1.3:12760894-12760913 | None:intergenic | 35.0% | |
| TTGACATGTTGGACAGATTA+TGG | - | chr1.3:12761476-12761495 | MS.gene067002:intron | 35.0% | |
| ! | ATGTTGTTTGCTGGAAGATT+TGG | - | chr1.3:12761516-12761535 | MS.gene067002:intron | 35.0% |
| ! | TAACGAGCATCGCAAAATTA+CGG | + | chr1.3:12761367-12761386 | None:intergenic | 35.0% |
| ! | TTTTATTCATAGTGCGTGTC+TGG | - | chr1.3:12761256-12761275 | MS.gene067002:intron | 35.0% |
| !! | GTTCTTGGTCTTAAGAACAT+TGG | - | chr1.3:12761687-12761706 | MS.gene067002:CDS | 35.0% |
| ACAACTTTGCCTCAATTCAC+TGG | - | chr1.3:12760843-12760862 | MS.gene067002:CDS | 40.0% | |
| ACATGTTGGACAGATTATGG+TGG | - | chr1.3:12761479-12761498 | MS.gene067002:intron | 40.0% | |
| AGTGGTTAATTACCAAACCC+TGG | + | chr1.3:12760899-12760918 | None:intergenic | 40.0% | |
| ! | ACAACCCTAATGTTGTTTGC+TGG | - | chr1.3:12761507-12761526 | MS.gene067002:intron | 40.0% |
| ! | ACAAGCCAAAGTATCAGCAA+GGG | + | chr1.3:12761634-12761653 | None:intergenic | 40.0% |
| ! | GTTTGCTGGAAGATTTGGAT+TGG | - | chr1.3:12761521-12761540 | MS.gene067002:intron | 40.0% |
| !! | ATAGGAGAAGGTTTGAGTTG+TGG | + | chr1.3:12760876-12760895 | None:intergenic | 40.0% |
| !! | ATTGGTGTTGGTGTTGTTCT+TGG | - | chr1.3:12761672-12761691 | MS.gene067002:CDS | 40.0% |
| !! | CATTGGTTCATCCACTAATG+TGG | - | chr1.3:12761200-12761219 | MS.gene067002:intron | 40.0% |
| ACTCAAACCTTCTCCTATCC+AGG | - | chr1.3:12760878-12760897 | MS.gene067002:CDS | 45.0% | |
| AGGACTAAAGCTGGAGACAA+GGG | - | chr1.3:12761572-12761591 | MS.gene067002:intron | 45.0% | |
| CTCAAACCTTCTCCTATCCA+GGG | - | chr1.3:12760879-12760898 | MS.gene067002:CDS | 45.0% | |
| GGAACTGTTGGACACATCAT+TGG | - | chr1.3:12761654-12761673 | MS.gene067002:CDS | 45.0% | |
| GGAGTTCGTTGTGACTTCAT+TGG | - | chr1.3:12761183-12761202 | MS.gene067002:intron | 45.0% | |
| GTTCACCCTTGCTGATACTT+TGG | - | chr1.3:12761626-12761645 | MS.gene067002:intron | 45.0% | |
| GTTGGACACATCATTGGTGT+TGG | - | chr1.3:12761660-12761679 | MS.gene067002:CDS | 45.0% | |
| ! | CACAAGCCAAAGTATCAGCA+AGG | + | chr1.3:12761635-12761654 | None:intergenic | 45.0% |
| ! | GGTCTAAGACCAGTGAATTG+AGG | + | chr1.3:12760855-12760874 | None:intergenic | 45.0% |
| ! | GTTGCTTTCCTGTTTGCTGA+GGG | + | chr1.3:12761549-12761568 | None:intergenic | 45.0% |
| !! | ACTTTGGCTTGTGGAACTGT+TGG | - | chr1.3:12761642-12761661 | MS.gene067002:CDS | 45.0% |
| !! | CTTGCTGATACTTTGGCTTG+TGG | - | chr1.3:12761633-12761652 | MS.gene067002:intron | 45.0% |
| !! | TGTTGCTTTCCTGTTTGCTG+AGG | + | chr1.3:12761550-12761569 | None:intergenic | 45.0% |
| ACCAAACCCTGGATAGGAGA+AGG | + | chr1.3:12760888-12760907 | None:intergenic | 50.0% | |
| ACCTTCTCCTATCCAGGGTT+TGG | - | chr1.3:12760884-12760903 | MS.gene067002:CDS | 50.0% | |
| AGCAACAGCAGGACTAAAGC+TGG | - | chr1.3:12761563-12761582 | MS.gene067002:intron | 50.0% | |
| CAACCTATGACAAGGCGCAA+GGG | - | chr1.3:12761147-12761166 | MS.gene067002:intron | 50.0% | |
| CAGGACTAAAGCTGGAGACA+AGG | - | chr1.3:12761571-12761590 | MS.gene067002:intron | 50.0% | |
| CTTGCGCCTTGTCATAGGTT+GGG | + | chr1.3:12761148-12761167 | None:intergenic | 50.0% | |
| GCAAACAGGAAAGCAACAGC+AGG | - | chr1.3:12761552-12761571 | MS.gene067002:intron | 50.0% | |
| TTGCTGCCCAACCTATGACA+AGG | - | chr1.3:12761139-12761158 | MS.gene067002:intron | 50.0% | |
| AAGCTGGAGACAAGGGACTC+AGG | - | chr1.3:12761579-12761598 | MS.gene067002:intron | 55.0% | |
| ATGACAAGGCGCAAGGGCAA+GGG | - | chr1.3:12761153-12761172 | MS.gene067002:intron | 55.0% | |
| CCAACCTATGACAAGGCGCA+AGG | - | chr1.3:12761146-12761165 | MS.gene067002:intron | 55.0% | |
| CCTTGCGCCTTGTCATAGGT+TGG | + | chr1.3:12761149-12761168 | None:intergenic | 55.0% | |
| TATGACAAGGCGCAAGGGCA+AGG | - | chr1.3:12761152-12761171 | MS.gene067002:intron | 55.0% | |
| TTGCCCTTGCGCCTTGTCAT+AGG | + | chr1.3:12761153-12761172 | None:intergenic | 55.0% | |
| ! | GATTGGCTCCCTCAGCAAAC+AGG | - | chr1.3:12761538-12761557 | MS.gene067002:intron | 55.0% |
| AGGGACTCAGGCTTGCAGAC+TGG | - | chr1.3:12761591-12761610 | MS.gene067002:intron | 60.0% | |
| CAGCAAGGGTGAACCCAGCT+GGG | + | chr1.3:12761620-12761639 | None:intergenic | 60.0% | |
| TCAGCAAGGGTGAACCCAGC+TGG | + | chr1.3:12761621-12761640 | None:intergenic | 60.0% | |
| TGCAGACTGGTGACCCAGCT+GGG | - | chr1.3:12761604-12761623 | MS.gene067002:intron | 60.0% | |
| TTGCAGACTGGTGACCCAGC+TGG | - | chr1.3:12761603-12761622 | MS.gene067002:intron | 60.0% | |
| !! | CGCAAGGGCAAGGGTGCTTT+GGG | - | chr1.3:12761162-12761181 | MS.gene067002:intron | 60.0% |
| !! | GCGCAAGGGCAAGGGTGCTT+TGG | - | chr1.3:12761161-12761180 | MS.gene067002:intron | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.3 | gene | 12760825 | 12761719 | 12760825 | ID=MS.gene067002 |
| chr1.3 | mRNA | 12760825 | 12761719 | 12760825 | ID=MS.gene067002.t1;Parent=MS.gene067002 |
| chr1.3 | exon | 12761639 | 12761719 | 12761639 | ID=MS.gene067002.t1.exon1;Parent=MS.gene067002.t1 |
| chr1.3 | CDS | 12761639 | 12761719 | 12761639 | ID=cds.MS.gene067002.t1;Parent=MS.gene067002.t1 |
| chr1.3 | exon | 12761323 | 12761406 | 12761323 | ID=MS.gene067002.t1.exon2;Parent=MS.gene067002.t1 |
| chr1.3 | CDS | 12761323 | 12761406 | 12761323 | ID=cds.MS.gene067002.t1;Parent=MS.gene067002.t1 |
| chr1.3 | exon | 12760825 | 12761052 | 12760825 | ID=MS.gene067002.t1.exon3;Parent=MS.gene067002.t1 |
| chr1.3 | CDS | 12760825 | 12761052 | 12760825 | ID=cds.MS.gene067002.t1;Parent=MS.gene067002.t1 |
| Gene Sequence |
| Protein sequence |
