AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene23137


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene23137.t1 MTR_7g111880 96.000 250 9 1 1 249 1 250 3.30e-172 474
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene23137.t1 AT4G15510 64.659 249 76 5 10 247 40 287 4.09e-105 306
MS.gene23137.t1 AT4G15510 73.039 204 52 2 46 247 3 205 2.02e-103 298
MS.gene23137.t1 AT4G15510 73.039 204 52 2 46 247 3 205 2.02e-103 298
MS.gene23137.t1 AT4G15510 73.039 204 52 2 46 247 3 205 2.02e-103 298
MS.gene23137.t1 AT4G15510 69.595 148 40 3 35 178 72 218 1.67e-62 196

Find 0 sgRNAs with CRISPR-Local

Find 72 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATTCTTGATGAAAATATAT+CGG + 51770:10447-10466 MS.gene23137:CDS 15.0%
!!! ATATTTTCATCAAGAATATA+AGG - 51770:10445-10464 None:intergenic 15.0%
!! AAATAAAATAATTGTGACAG+AGG - 51770:10173-10192 None:intergenic 20.0%
!! TACATTTATCAGAAAAATTG+TGG + 51770:10674-10693 MS.gene23137:intron 20.0%
! GAATATGTTGATACATTTGA+TGG + 51770:10349-10368 MS.gene23137:CDS 25.0%
AAAACTTACGGTAATTTGAG+GGG + 51770:10722-10741 MS.gene23137:intron 30.0%
AGAAAATGTGTTTCAAGAAG+AGG + 51770:10991-11010 MS.gene23137:CDS 30.0%
CAAAACTTACGGTAATTTGA+GGG + 51770:10721-10740 MS.gene23137:intron 30.0%
CATTCAACTATCCATCAAAT+TGG + 51770:10377-10396 MS.gene23137:CDS 30.0%
CTTACTTGAATCCAATTTGA+TGG - 51770:10391-10410 None:intergenic 30.0%
TCAAATTGGATTCAAGTAAG+AGG + 51770:10391-10410 MS.gene23137:CDS 30.0%
! CAAATTGTACTATCAAGTTG+AGG + 51770:10645-10664 MS.gene23137:CDS 30.0%
! TCTTCTTGAAACACATTTTC+TGG - 51770:10992-11011 None:intergenic 30.0%
!! AATGACATTGCTTTTCTTCT+AGG - 51770:10262-10281 None:intergenic 30.0%
ATGGATGGAATTTACCGAAT+TGG - 51770:10136-10155 None:intergenic 35.0%
ATTCAAGACTGTTCAAGACT+TGG + 51770:10498-10517 MS.gene23137:CDS 35.0%
CTCTGTTCTTGCAAAACTTA+CGG + 51770:10710-10729 MS.gene23137:intron 35.0%
GCAAAACTTACGGTAATTTG+AGG + 51770:10720-10739 MS.gene23137:intron 35.0%
GTAATCTCAACTCATACAGT+TGG - 51770:10964-10983 None:intergenic 35.0%
GTCTTGAATTTCGAAGAAGA+AGG - 51770:10487-10506 None:intergenic 35.0%
TGGATGGAATTTACCGAATT+GGG - 51770:10135-10154 None:intergenic 35.0%
TTACTGAGTTTATGTCCACT+AGG + 51770:10563-10582 MS.gene23137:CDS 35.0%
TTCAAGACTGTTCAAGACTT+GGG + 51770:10499-10518 MS.gene23137:CDS 35.0%
! GGGTGTCTTTTATATGGTAA+TGG + 51770:10742-10761 MS.gene23137:intron 35.0%
!!! TGGTGCTGATATTTTCTTCA+GGG + 51770:10417-10436 MS.gene23137:CDS 35.0%
!!! TTTGAGGGGTGTCTTTTATA+TGG + 51770:10736-10755 MS.gene23137:intron 35.0%
ACATATTCACGAAACACACG+AGG - 51770:10337-10356 None:intergenic 40.0%
ACCCGTTGTTGTGATTGTAT+TGG - 51770:10049-10068 None:intergenic 40.0%
ACTTACATTCTCTCCGACAT+TGG + 51770:10292-10311 MS.gene23137:CDS 40.0%
AGAGGAGAAAGCTCATGAAA+TGG - 51770:10155-10174 None:intergenic 40.0%
ATGGAAGTGAAATGGTGACA+TGG - 51770:10085-10104 None:intergenic 40.0%
GAATTTACCGAATTGGGTGT+GGG - 51770:10129-10148 None:intergenic 40.0%
GAGAAAGCTCATGAAATGGA+TGG - 51770:10151-10170 None:intergenic 40.0%
GCCAATACAATCACAACAAC+GGG + 51770:10045-10064 MS.gene23137:CDS 40.0%
GGCCAATACAATCACAACAA+CGG + 51770:10044-10063 MS.gene23137:CDS 40.0%
TAGCGCTAGACTACAATGTA+TGG - 51770:10770-10789 None:intergenic 40.0%
TGCTAATGCTGTACCAATGT+CGG - 51770:10308-10327 None:intergenic 40.0%
TGCTGTTATGCCACAAGATA+GGG + 51770:10883-10902 MS.gene23137:CDS 40.0%
TGGAAGTGAAATGGTGACAT+GGG - 51770:10084-10103 None:intergenic 40.0%
TTTCAACTCCTAGAACAGAC+AGG - 51770:10937-10956 None:intergenic 40.0%
! TTGCTGTTATGCCACAAGAT+AGG + 51770:10882-10901 MS.gene23137:CDS 40.0%
!! TGTGTGTTGTTTGTGTTGCT+AGG + 51770:10826-10845 MS.gene23137:intron 40.0%
!!! CTGGTGCTGATATTTTCTTC+AGG + 51770:10416-10435 MS.gene23137:CDS 40.0%
ACGTTTAACACCAAGCCTAG+TGG - 51770:10581-10600 None:intergenic 45.0%
AGTGAAATGGTGACATGGGT+CGG - 51770:10080-10099 None:intergenic 45.0%
ATCATCAGCAACTCTCTCAG+AGG - 51770:10623-10642 None:intergenic 45.0%
CATTTCTCCCACACCCAATT+CGG + 51770:10119-10138 MS.gene23137:CDS 45.0%
GAGTGAACTTCGTCAAGTCA+TGG + 51770:11015-11034 MS.gene23137:CDS 45.0%
GAGTTTATGTCCACTAGGCT+TGG + 51770:10568-10587 MS.gene23137:CDS 45.0%
GGAATTTACCGAATTGGGTG+TGG - 51770:10130-10149 None:intergenic 45.0%
GGCTTCGTTTCGAGCAAATA+AGG + 51770:11036-11055 MS.gene23137:CDS 45.0%
TCTGAGAGAGTTGCTGATGA+TGG + 51770:10622-10641 MS.gene23137:CDS 45.0%
TGTTATGCCACAAGATAGGG+TGG + 51770:10886-10905 MS.gene23137:CDS 45.0%
! TGGATTCAAGTAAGAGGTGC+TGG + 51770:10397-10416 MS.gene23137:CDS 45.0%
!! ACTTTCTTCCCAGCTTGTTG+AGG - 51770:10529-10548 None:intergenic 45.0%
AAACGCACCACCCTATCTTG+TGG - 51770:10896-10915 None:intergenic 50.0%
ACAAGATAGGGTGGTGCGTT+TGG + 51770:10895-10914 MS.gene23137:CDS 50.0%
ATAGGGTGGTGCGTTTGGAA+TGG + 51770:10900-10919 MS.gene23137:CDS 50.0%
ATCAGCAACTCTCTCAGAGG+TGG - 51770:10620-10639 None:intergenic 50.0%
CGAATTGGGTGTGGGAGAAA+TGG - 51770:10121-10140 None:intergenic 50.0%
CGAGGGGAATGGAAGTGAAA+TGG - 51770:10093-10112 None:intergenic 50.0%
GTGAAATGGTGACATGGGTC+GGG - 51770:10079-10098 None:intergenic 50.0%
! TAGGGTGGTGCGTTTGGAAT+GGG + 51770:10901-10920 MS.gene23137:CDS 50.0%
! TGCGTTTGGAATGGGATAGG+AGG + 51770:10909-10928 MS.gene23137:CDS 50.0%
! TGGTGCGTTTGGAATGGGAT+AGG + 51770:10906-10925 MS.gene23137:CDS 50.0%
!! AGGAGGTACCTGTCTGTTCT+AGG + 51770:10926-10945 MS.gene23137:CDS 50.0%
AAATGGAGCAGCGAGGGGAA+TGG - 51770:10104-10123 None:intergenic 55.0%
TGGGAGAAATGGAGCAGCGA+GGG - 51770:10110-10129 None:intergenic 55.0%
TGGGTTCGCCTCAACAAGCT+GGG + 51770:10518-10537 MS.gene23137:CDS 55.0%
TTGGGTTCGCCTCAACAAGC+TGG + 51770:10517-10536 MS.gene23137:CDS 55.0%
GGGAGAAATGGAGCAGCGAG+GGG - 51770:10109-10128 None:intergenic 60.0%
GTGGGAGAAATGGAGCAGCG+AGG - 51770:10111-10130 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
51770 gene 10042 11069 10042 ID=MS.gene23137
51770 mRNA 10042 11069 10042 ID=MS.gene23137.t1;Parent=MS.gene23137
51770 exon 10042 10140 10042 ID=MS.gene23137.t1.exon1;Parent=MS.gene23137.t1
51770 CDS 10042 10140 10042 ID=cds.MS.gene23137.t1;Parent=MS.gene23137.t1
51770 exon 10238 10666 10238 ID=MS.gene23137.t1.exon2;Parent=MS.gene23137.t1
51770 CDS 10238 10666 10238 ID=cds.MS.gene23137.t1;Parent=MS.gene23137.t1
51770 exon 10848 11069 10848 ID=MS.gene23137.t1.exon3;Parent=MS.gene23137.t1
51770 CDS 10848 11069 10848 ID=cds.MS.gene23137.t1;Parent=MS.gene23137.t1
Gene Sequence

>MS.gene23137

ATGGCCAATACAATCACAACAACGGGTTTTCTAGCCCGACCCATGTCACCATTTCACTTCCATTCCCCTCGCTGCTCCATTTCTCCCACACCCAATTCGGTAAATTCCATCCATTTCATGAGCTTTCTCCTCTGTCACAATTATTTTATTTTATTTACTAAATCTTTGATTTTTGTCTCTGCTAATATATAATCAGATTAAAGCAGCTTTTGCAGTTCCTAGAAGAAAAGCAATGTCATTAATCTTATCAACTTACATTCTCTCCGACATTGGTACAGCATTAGCACAACAACCTCGTGTGTTTCGTGAATATGTTGATACATTTGATGGTTATTCATTCAACTATCCATCAAATTGGATTCAAGTAAGAGGTGCTGGTGCTGATATTTTCTTCAGGGATCCTTATATTCTTGATGAAAATATATCGGTCGAAGTTTCTTCGCCTTCTTCTTCGAAATTCAAGACTGTTCAAGACTTGGGTTCGCCTCAACAAGCTGGGAAGAAAGTTCTTGATCAGTATCTTACTGAGTTTATGTCCACTAGGCTTGGTGTTAAACGTGAATCTAATATTCTTTCCACCTCTGAGAGAGTTGCTGATGATGGCAAATTGTACTATCAAGTTGAGGTTCGTTTACATTTATCAGAAAAATTGTGGTTAATCACGTTAACTCTGTTCTTGCAAAACTTACGGTAATTTGAGGGGTGTCTTTTATATGGTAATGGAACCATACATTGTAGTCTAGCGCTACTTGTTGCAAAATGACATTAAATGATTGATTGATTGTGTGTGTTGTTTGTGTTGCTAGGCGAACATAAAGTCATATGCTAGTAACAACGAGCTTGCTGTTATGCCACAAGATAGGGTGGTGCGTTTGGAATGGGATAGGAGGTACCTGTCTGTTCTAGGAGTTGAAAACAACCAACTGTATGAGTTGAGATTACAAGTGCCAGAAAATGTGTTTCAAGAAGAGGAGAGTGAACTTCGTCAAGTCATGGCTTCGTTTCGAGCAAATAAGGTTGTTCGTTAG

Protein sequence

>MS.gene23137.t1

MANTITTTGFLARPMSPFHFHSPRCSISPTPNSIKAAFAVPRRKAMSLILSTYILSDIGTALAQQPRVFREYVDTFDGYSFNYPSNWIQVRGAGADIFFRDPYILDENISVEVSSPSSSKFKTVQDLGSPQQAGKKVLDQYLTEFMSTRLGVKRESNILSTSERVADDGKLYYQVEANIKSYASNNELAVMPQDRVVRLEWDRRYLSVLGVENNQLYELRLQVPENVFQEEESELRQVMASFRANKVVR