AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene68729


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene68729.t1 MTR_3g118210 99.558 226 1 0 1 226 47 272 9.90e-165 455
MS.gene68729.t1 MTR_6g006970 42.718 206 114 4 12 216 49 251 9.85e-47 156
MS.gene68729.t1 MTR_1g093060 40.777 206 118 4 12 216 102 304 1.06e-42 147
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene68729.t1 AT2G43030 82.743 226 39 0 1 226 46 271 4.47e-130 368
MS.gene68729.t1 AT3G17465 37.037 216 133 3 11 225 77 290 2.47e-38 135

Find 78 sgRNAs with CRISPR-Local

Find 89 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTAACAATCTTAAGCTTTC+TGG 0.097361 3.2:-93188162 None:intergenic
AAAGAGACTAATTAATTCTT+TGG 0.209215 3.2:-93188287 None:intergenic
AAAGCTCATCATTCCCAATT+TGG 0.228676 3.2:-93187572 None:intergenic
CAAATTGACCAAACCTGAAA+TGG 0.282604 3.2:+93187729 MS.gene68729:CDS
CATTCCTATGCGACATCTTC+AGG 0.315485 3.2:+93187774 MS.gene68729:CDS
ATGATCAAAGGTGCTCTTCC+TGG 0.365904 3.2:+93188209 MS.gene68729:CDS
TTTCGTCTTCAATCCGTTGA+TGG 0.384156 3.2:+93187799 MS.gene68729:CDS
TCAAATACAAGGCGCTGATT+AGG 0.398930 3.2:-93187829 None:intergenic
AAGATGACCCATTTCAGGTT+TGG 0.399688 3.2:-93187737 None:intergenic
CTCTTGGAACCATGGCTCAT+TGG 0.403785 3.2:-93188041 None:intergenic
TGGTGTGATGGGTACCAAAT+TGG 0.414953 3.2:+93187558 MS.gene68729:CDS
GTTGTTCCCGTCACTGTTGT+CGG 0.415769 3.2:+93187613 MS.gene68729:CDS
GGTGATCTTGTTGATGTCTC+TGG 0.428399 3.2:+93187868 MS.gene68729:CDS
AAGCTTTCTGGTCTTCCTGT+TGG 0.434373 3.2:-93188150 None:intergenic
ACGCCGTCCAAGTTGGTTAC+CGG 0.438083 3.2:+93187692 MS.gene68729:CDS
TCCAAGTTGTCTGTGACTCT+TGG 0.442433 3.2:-93188057 None:intergenic
TCCAAGAGTCACAGACAACT+TGG 0.460086 3.2:+93188056 MS.gene68729:CDS
TTCTTGCCAACAATCTTAGC+GGG 0.460342 3.2:-93188260 None:intergenic
ACTGTTGTCGGTTTCAAAGA+AGG 0.461503 3.2:+93187625 MS.gene68729:CDS
AGAGGCTGGTGTTGGTGTGA+TGG 0.463975 3.2:+93187546 MS.gene68729:CDS
TACACCTGGTCGCGTATACA+AGG 0.469173 3.2:+93188097 MS.gene68729:CDS
TTTCAGGTTTGGTCAATTTG+CGG 0.469193 3.2:-93187726 None:intergenic
GTTGTAACCGTCGGTGGCGT+CGG 0.473671 3.2:-93187671 None:intergenic
AAAGAAGATGCCTGGTAGGA+TGG 0.474224 3.2:+93188121 MS.gene68729:CDS
GAGGCTGGTGTTGGTGTGAT+GGG 0.474712 3.2:+93187547 MS.gene68729:CDS
TAACCCTCCGGTAACCAACT+TGG 0.478957 3.2:-93187699 None:intergenic
CACAGACAACTTGGATCCAT+TGG 0.481197 3.2:+93188065 MS.gene68729:CDS
GTAGGATGGGTGGAACCAAC+AGG 0.493432 3.2:+93188135 MS.gene68729:CDS
TACGCGACCAGGTGTAGTTC+CGG 0.494636 3.2:-93188090 None:intergenic
TTTCCCTTGTATACGCGACC+AGG 0.499382 3.2:-93188101 None:intergenic
AGAAGATTACCAGGCTTACC+AGG 0.500896 3.2:-93188227 None:intergenic
TGAAATGGGTCATCTTGAAA+AGG 0.503372 3.2:+93187744 MS.gene68729:CDS
ACGGGAACAACTTCACCGTT+AGG 0.508452 3.2:-93187601 None:intergenic
AAGAAGATGCCTGGTAGGAT+GGG 0.509948 3.2:+93188122 MS.gene68729:CDS
GGTGTGATGGGTACCAAATT+GGG 0.512034 3.2:+93187559 MS.gene68729:CDS
AAATTGACCAAACCTGAAAT+GGG 0.513032 3.2:+93187730 MS.gene68729:CDS
TTGAAACCGACAACAGTGAC+GGG 0.513527 3.2:-93187619 None:intergenic
GGTGCTCTTCCTGGTAAGCC+TGG 0.514459 3.2:+93188218 MS.gene68729:CDS
AAGCGTCATAACTTCAAAAG+AGG 0.517261 3.2:+93188017 MS.gene68729:CDS
AGGTGTAGTTCCGGCACCAA+TGG 0.518535 3.2:-93188081 None:intergenic
TTGGTTCCACCCATCCTACC+AGG 0.519178 3.2:-93188131 None:intergenic
ATGGGTCATCTTGAAAAGGT+TGG 0.519826 3.2:+93187748 MS.gene68729:CDS
GATGTCTCTGGCACCACCAT+CGG 0.522268 3.2:+93187880 MS.gene68729:CDS
GGTGGTGAAAATGAGCTTAG+AGG 0.522460 3.2:+93187528 MS.gene68729:CDS
GTAAGCCTGGTAATCTTCTA+CGG 0.530779 3.2:+93188231 MS.gene68729:CDS
GAACAACTTCACCGTTAGGT+TGG 0.533148 3.2:-93187597 None:intergenic
CTCTGGCACCACCATCGGCA+AGG 0.534330 3.2:+93187885 MS.gene68729:CDS
TACAAGGGAAAGAAGATGCC+TGG 0.536322 3.2:+93188113 MS.gene68729:CDS
GTCTGTGACTCTTGGAACCA+TGG 0.543733 3.2:-93188049 None:intergenic
CTGAAGATGTCGCATAGGAA+TGG 0.560330 3.2:-93187773 None:intergenic
CAACTTGGATCCATTGGTGC+CGG 0.569215 3.2:+93188071 MS.gene68729:CDS
ATTTGCGGTCTCTAACCCTC+CGG 0.569415 3.2:-93187711 None:intergenic
ATTAGGTTCAAAACCATCAA+CGG 0.570807 3.2:-93187812 None:intergenic
TCTGGCACCACCATCGGCAA+GGG 0.572275 3.2:+93187886 MS.gene68729:CDS
CTTAATGTTATCATGATCAA+AGG 0.572918 3.2:+93188197 MS.gene68729:CDS
ATTGGTGCCGGAACTACACC+TGG 0.580799 3.2:+93188083 MS.gene68729:CDS
TTTGAAACCGACAACAGTGA+CGG 0.586413 3.2:-93187620 None:intergenic
CCGTCCAAGTTGGTTACCGG+AGG 0.591201 3.2:+93187695 MS.gene68729:CDS
GTTCTTGCCAACAATCTTAG+CGG 0.594511 3.2:-93188261 None:intergenic
AAGATGCCTGGTAGGATGGG+TGG 0.594546 3.2:+93188125 MS.gene68729:CDS
ACCTTGAAAACCCTTGCCGA+TGG 0.600155 3.2:-93187896 None:intergenic
TGAAAAGCTCATCAAATACA+AGG 0.600821 3.2:-93187840 None:intergenic
GCTATCCGTAGAAGATTACC+AGG 0.602366 3.2:-93188236 None:intergenic
AGGGAAAGAAGATGCCTGGT+AGG 0.605040 3.2:+93188117 MS.gene68729:CDS
TTGAAAACCCTTGCCGATGG+TGG 0.607518 3.2:-93187893 None:intergenic
ATCAAAACCGACGCCACCGA+CGG 0.609949 3.2:+93187664 MS.gene68729:CDS
GGTTACAACGCCGTCCAAGT+TGG 0.618166 3.2:+93187685 MS.gene68729:CDS
GTGAAAATGAGCTTAGAGGC+TGG 0.634928 3.2:+93187532 MS.gene68729:CDS
TCTTGCCAACAATCTTAGCG+GGG 0.638407 3.2:-93188259 None:intergenic
CCTCCGGTAACCAACTTGGA+CGG 0.639997 3.2:-93187695 None:intergenic
ATGAGCTTAGAGGCTGGTGT+TGG 0.640133 3.2:+93187538 MS.gene68729:CDS
AAAAGAGGACCAATGAGCCA+TGG 0.640937 3.2:+93188032 MS.gene68729:CDS
CGTCCAAGTTGGTTACCGGA+GGG 0.643249 3.2:+93187696 MS.gene68729:CDS
CTTGCCAACAATCTTAGCGG+GGG 0.643846 3.2:-93188258 None:intergenic
GACGGCGTTGTAACCGTCGG+TGG 0.648118 3.2:-93187677 None:intergenic
AACTCCTGAAGATGTCGCAT+AGG 0.655881 3.2:-93187778 None:intergenic
TTGGACGGCGTTGTAACCGT+CGG 0.663802 3.2:-93187680 None:intergenic
ACACCTGGTCGCGTATACAA+GGG 0.687415 3.2:+93188098 MS.gene68729:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAATAATATCTTTCACTAA+TGG + chr3.2:93187964-93187983 MS.gene68729:intron 15.0%
! CTTAATGTTATCATGATCAA+AGG + chr3.2:93188197-93188216 MS.gene68729:CDS 25.0%
! TTTAACAATCTTAAGCTTTC+TGG - chr3.2:93188165-93188184 None:intergenic 25.0%
AAATTGACCAAACCTGAAAT+GGG + chr3.2:93187730-93187749 MS.gene68729:CDS 30.0%
ATGAACTTACTTACTTTGAC+AGG + chr3.2:93187987-93188006 MS.gene68729:intron 30.0%
ATTAGGTTCAAAACCATCAA+CGG - chr3.2:93187815-93187834 None:intergenic 30.0%
TATCAACTCAACAACTAGAA+GGG - chr3.2:93187947-93187966 None:intergenic 30.0%
TGAAAAGCTCATCAAATACA+AGG - chr3.2:93187843-93187862 None:intergenic 30.0%
TTATCAACTCAACAACTAGA+AGG - chr3.2:93187948-93187967 None:intergenic 30.0%
! TTTGATGAGCTTTTCAATGA+AGG + chr3.2:93187847-93187866 MS.gene68729:CDS 30.0%
AAAGCTCATCATTCCCAATT+TGG - chr3.2:93187575-93187594 None:intergenic 35.0%
AACTTACTTACTTTGACAGG+TGG + chr3.2:93187990-93188009 MS.gene68729:intron 35.0%
AAGCGTCATAACTTCAAAAG+AGG + chr3.2:93188017-93188036 MS.gene68729:CDS 35.0%
CAAATTGACCAAACCTGAAA+TGG + chr3.2:93187729-93187748 MS.gene68729:CDS 35.0%
TGAAATGGGTCATCTTGAAA+AGG + chr3.2:93187744-93187763 MS.gene68729:CDS 35.0%
!! ATGAGCTTTTTCCAACCTAA+CGG + chr3.2:93187586-93187605 MS.gene68729:CDS 35.0%
!! TTTCAGGTTTGGTCAATTTG+CGG - chr3.2:93187729-93187748 None:intergenic 35.0%
!! TTTTCAAGATGACCCATTTC+AGG - chr3.2:93187745-93187764 None:intergenic 35.0%
AACTAGAAGGGAATGCAAAG+AGG - chr3.2:93187935-93187954 None:intergenic 40.0%
ACTAGAAGGGAATGCAAAGA+GGG - chr3.2:93187934-93187953 None:intergenic 40.0%
ATGGGTCATCTTGAAAAGGT+TGG + chr3.2:93187748-93187767 MS.gene68729:CDS 40.0%
GTAAGCCTGGTAATCTTCTA+CGG + chr3.2:93188231-93188250 MS.gene68729:CDS 40.0%
GTTCTTGCCAACAATCTTAG+CGG - chr3.2:93188264-93188283 None:intergenic 40.0%
TTCTTGCCAACAATCTTAGC+GGG - chr3.2:93188263-93188282 None:intergenic 40.0%
TTTCGTCTTCAATCCGTTGA+TGG + chr3.2:93187799-93187818 MS.gene68729:CDS 40.0%
! TTTGAAACCGACAACAGTGA+CGG - chr3.2:93187623-93187642 None:intergenic 40.0%
!! AAGATGACCCATTTCAGGTT+TGG - chr3.2:93187740-93187759 None:intergenic 40.0%
!! ACTGTTGTCGGTTTCAAAGA+AGG + chr3.2:93187625-93187644 MS.gene68729:CDS 40.0%
!! TCAAATACAAGGCGCTGATT+AGG - chr3.2:93187832-93187851 None:intergenic 40.0%
AAAAGAGGACCAATGAGCCA+TGG + chr3.2:93188032-93188051 MS.gene68729:CDS 45.0%
AAAGAAGATGCCTGGTAGGA+TGG + chr3.2:93188121-93188140 MS.gene68729:CDS 45.0%
AACTCCTGAAGATGTCGCAT+AGG - chr3.2:93187781-93187800 None:intergenic 45.0%
AAGAAGATGCCTGGTAGGAT+GGG + chr3.2:93188122-93188141 MS.gene68729:CDS 45.0%
AGAAGATTACCAGGCTTACC+AGG - chr3.2:93188230-93188249 None:intergenic 45.0%
CACAGACAACTTGGATCCAT+TGG + chr3.2:93188065-93188084 MS.gene68729:CDS 45.0%
CATTCCTATGCGACATCTTC+AGG + chr3.2:93187774-93187793 MS.gene68729:CDS 45.0%
CTGAAGATGTCGCATAGGAA+TGG - chr3.2:93187776-93187795 None:intergenic 45.0%
GAACAACTTCACCGTTAGGT+TGG - chr3.2:93187600-93187619 None:intergenic 45.0%
GCTATCCGTAGAAGATTACC+AGG - chr3.2:93188239-93188258 None:intergenic 45.0%
GGTGATCTTGTTGATGTCTC+TGG + chr3.2:93187868-93187887 MS.gene68729:CDS 45.0%
GGTGGTGAAAATGAGCTTAG+AGG + chr3.2:93187528-93187547 MS.gene68729:CDS 45.0%
GTGAAAATGAGCTTAGAGGC+TGG + chr3.2:93187532-93187551 MS.gene68729:CDS 45.0%
TACAAGGGAAAGAAGATGCC+TGG + chr3.2:93188113-93188132 MS.gene68729:CDS 45.0%
TCCAAGAGTCACAGACAACT+TGG + chr3.2:93188056-93188075 MS.gene68729:CDS 45.0%
TCCAAGTTGTCTGTGACTCT+TGG - chr3.2:93188060-93188079 None:intergenic 45.0%
TCTTGCCAACAATCTTAGCG+GGG - chr3.2:93188262-93188281 None:intergenic 45.0%
TTGAAACCGACAACAGTGAC+GGG - chr3.2:93187622-93187641 None:intergenic 45.0%
! AAGCTTTCTGGTCTTCCTGT+TGG - chr3.2:93188153-93188172 None:intergenic 45.0%
!! ATGATCAAAGGTGCTCTTCC+TGG + chr3.2:93188209-93188228 MS.gene68729:CDS 45.0%
!! GGTGTGATGGGTACCAAATT+GGG + chr3.2:93187559-93187578 MS.gene68729:CDS 45.0%
!! TGGTGTGATGGGTACCAAAT+TGG + chr3.2:93187558-93187577 MS.gene68729:CDS 45.0%
ACACCTGGTCGCGTATACAA+GGG + chr3.2:93188098-93188117 MS.gene68729:CDS 50.0%
ACCTTGAAAACCCTTGCCGA+TGG - chr3.2:93187899-93187918 None:intergenic 50.0%
ACGGGAACAACTTCACCGTT+AGG - chr3.2:93187604-93187623 None:intergenic 50.0%
AGGGAAAGAAGATGCCTGGT+AGG + chr3.2:93188117-93188136 MS.gene68729:CDS 50.0%
ATAGCCCCCGCTAAGATTGT+TGG + chr3.2:93188254-93188273 MS.gene68729:CDS 50.0%
ATGAGCTTAGAGGCTGGTGT+TGG + chr3.2:93187538-93187557 MS.gene68729:CDS 50.0%
ATTTGCGGTCTCTAACCCTC+CGG - chr3.2:93187714-93187733 None:intergenic 50.0%
CAACTTGGATCCATTGGTGC+CGG + chr3.2:93188071-93188090 MS.gene68729:CDS 50.0%
CTCTTGGAACCATGGCTCAT+TGG - chr3.2:93188044-93188063 None:intergenic 50.0%
CTTGCCAACAATCTTAGCGG+GGG - chr3.2:93188261-93188280 None:intergenic 50.0%
GTCTGTGACTCTTGGAACCA+TGG - chr3.2:93188052-93188071 None:intergenic 50.0%
GTTGTTCCCGTCACTGTTGT+CGG + chr3.2:93187613-93187632 MS.gene68729:CDS 50.0%
TAACCCTCCGGTAACCAACT+TGG - chr3.2:93187702-93187721 None:intergenic 50.0%
TACACCTGGTCGCGTATACA+AGG + chr3.2:93188097-93188116 MS.gene68729:CDS 50.0%
TTGAAAACCCTTGCCGATGG+TGG - chr3.2:93187896-93187915 None:intergenic 50.0%
TTTCCCTTGTATACGCGACC+AGG - chr3.2:93188104-93188123 None:intergenic 50.0%
! ACCATCGGCAAGGGTTTTCA+AGG + chr3.2:93187895-93187914 MS.gene68729:CDS 50.0%
AAGATGCCTGGTAGGATGGG+TGG + chr3.2:93188125-93188144 MS.gene68729:CDS 55.0%
AGGTGTAGTTCCGGCACCAA+TGG - chr3.2:93188084-93188103 None:intergenic 55.0%
ATCAAAACCGACGCCACCGA+CGG + chr3.2:93187664-93187683 MS.gene68729:CDS 55.0%
CCTCCGGTAACCAACTTGGA+CGG - chr3.2:93187698-93187717 None:intergenic 55.0%
GATGTCTCTGGCACCACCAT+CGG + chr3.2:93187880-93187899 MS.gene68729:CDS 55.0%
GGTTACAACGCCGTCCAAGT+TGG + chr3.2:93187685-93187704 MS.gene68729:CDS 55.0%
GTAGGATGGGTGGAACCAAC+AGG + chr3.2:93188135-93188154 MS.gene68729:CDS 55.0%
TACGCGACCAGGTGTAGTTC+CGG - chr3.2:93188093-93188112 None:intergenic 55.0%
TTGGACGGCGTTGTAACCGT+CGG - chr3.2:93187683-93187702 None:intergenic 55.0%
TTGGTTCCACCCATCCTACC+AGG - chr3.2:93188134-93188153 None:intergenic 55.0%
!! ACGCCGTCCAAGTTGGTTAC+CGG + chr3.2:93187692-93187711 MS.gene68729:CDS 55.0%
!! AGAGGCTGGTGTTGGTGTGA+TGG + chr3.2:93187546-93187565 MS.gene68729:CDS 55.0%
!! ATTGGTGCCGGAACTACACC+TGG + chr3.2:93188083-93188102 MS.gene68729:CDS 55.0%
!! CGTCCAAGTTGGTTACCGGA+GGG + chr3.2:93187696-93187715 MS.gene68729:CDS 55.0%
!! GAGGCTGGTGTTGGTGTGAT+GGG + chr3.2:93187547-93187566 MS.gene68729:CDS 55.0%
GGTGCTCTTCCTGGTAAGCC+TGG + chr3.2:93188218-93188237 MS.gene68729:CDS 60.0%
GTTGTAACCGTCGGTGGCGT+CGG - chr3.2:93187674-93187693 None:intergenic 60.0%
TCTGGCACCACCATCGGCAA+GGG + chr3.2:93187886-93187905 MS.gene68729:CDS 60.0%
!! CCGTCCAAGTTGGTTACCGG+AGG + chr3.2:93187695-93187714 MS.gene68729:CDS 60.0%
CTCTGGCACCACCATCGGCA+AGG + chr3.2:93187885-93187904 MS.gene68729:CDS 65.0%
GACGGCGTTGTAACCGTCGG+TGG - chr3.2:93187680-93187699 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr3.2 gene 93187526 93188298 93187526 ID=MS.gene68729
chr3.2 mRNA 93187526 93188298 93187526 ID=MS.gene68729.t1;Parent=MS.gene68729
chr3.2 exon 93187526 93187916 93187526 ID=MS.gene68729.t1.exon1;Parent=MS.gene68729.t1
chr3.2 CDS 93187526 93187916 93187526 ID=cds.MS.gene68729.t1;Parent=MS.gene68729.t1
chr3.2 exon 93188009 93188298 93188009 ID=MS.gene68729.t1.exon2;Parent=MS.gene68729.t1
chr3.2 CDS 93188009 93188298 93188009 ID=cds.MS.gene68729.t1;Parent=MS.gene68729.t1
Gene Sequence

>MS.gene68729

ATGGTGGTGAAAATGAGCTTAGAGGCTGGTGTTGGTGTGATGGGTACCAAATTGGGAATGATGAGCTTTTTCCAACCTAACGGTGAAGTTGTTCCCGTCACTGTTGTCGGTTTCAAAGAAGGCAACATCGTCACTCAAATCAAAACCGACGCCACCGACGGTTACAACGCCGTCCAAGTTGGTTACCGGAGGGTTAGAGACCGCAAATTGACCAAACCTGAAATGGGTCATCTTGAAAAGGTTGGAGCCATTCCTATGCGACATCTTCAGGAGTTTCGTCTTCAATCCGTTGATGGTTTTGAACCTAATCAGCGCCTTGTATTTGATGAGCTTTTCAATGAAGGTGATCTTGTTGATGTCTCTGGCACCACCATCGGCAAGGGTTTTCAAGGTCTGTAACTCTCTCCCTCTTTGCATTCCCTTCTAGTTGTTGAGTTGATAATAATATCTTTCACTAATGGATGAACTTACTTACTTTGACAGGTGGTATCAAGCGTCATAACTTCAAAAGAGGACCAATGAGCCATGGTTCCAAGAGTCACAGACAACTTGGATCCATTGGTGCCGGAACTACACCTGGTCGCGTATACAAGGGAAAGAAGATGCCTGGTAGGATGGGTGGAACCAACAGGAAGACCAGAAAGCTTAAGATTGTTAAAATTGATAAAGATCTTAATGTTATCATGATCAAAGGTGCTCTTCCTGGTAAGCCTGGTAATCTTCTACGGATAGCCCCCGCTAAGATTGTTGGCAAGAACATTCCAAAGAATTAA

Protein sequence

>MS.gene68729.t1

MVVKMSLEAGVGVMGTKLGMMSFFQPNGEVVPVTVVGFKEGNIVTQIKTDATDGYNAVQVGYRRVRDRKLTKPEMGHLEKVGAIPMRHLQEFRLQSVDGFEPNQRLVFDELFNEGDLVDVSGTTIGKGFQGGIKRHNFKRGPMSHGSKSHRQLGSIGAGTTPGRVYKGKKMPGRMGGTNRKTRKLKIVKIDKDLNVIMIKGALPGKPGNLLRIAPAKIVGKNIPKN