Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene33873.t1 | XP_013466249.1 | 96.9 | 130 | 4 | 0 | 1 | 130 | 1 | 130 | 4.40E-61 | 243.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene33873.t1 | Q9ZT05 | 96.9 | 127 | 4 | 0 | 1 | 127 | 1 | 127 | 6.8e-63 | 241.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene33873.t1 | I3SQF1 | 96.9 | 130 | 4 | 0 | 1 | 130 | 1 | 130 | 3.1e-61 | 243.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049346 | MS.gene33873 | 0.827685 | 1.37E-54 | -1.69E-46 |
| MS.gene049351 | MS.gene33873 | 0.831453 | 1.66E-55 | -1.69E-46 |
| MS.gene049427 | MS.gene33873 | 0.818909 | 1.53E-52 | -1.69E-46 |
| MS.gene049491 | MS.gene33873 | 0.830793 | 2.40E-55 | -1.69E-46 |
| MS.gene049609 | MS.gene33873 | 0.804689 | 1.92E-49 | -1.69E-46 |
| MS.gene049614 | MS.gene33873 | 0.802294 | 6.01E-49 | -1.69E-46 |
| MS.gene049661 | MS.gene33873 | 0.881815 | 1.75E-70 | -1.69E-46 |
| MS.gene049662 | MS.gene33873 | 0.876353 | 1.49E-68 | -1.69E-46 |
| MS.gene049758 | MS.gene33873 | 0.850556 | 1.57E-60 | -1.69E-46 |
| MS.gene049760 | MS.gene33873 | 0.825903 | 3.64E-54 | -1.69E-46 |
| MS.gene049805 | MS.gene33873 | 0.811089 | 8.34E-51 | -1.69E-46 |
| MS.gene050076 | MS.gene33873 | 0.886222 | 4.13E-72 | -1.69E-46 |
| MS.gene050099 | MS.gene33873 | 0.810715 | 1.00E-50 | -1.69E-46 |
| MS.gene050128 | MS.gene33873 | 0.813729 | 2.21E-51 | -1.69E-46 |
| MS.gene050301 | MS.gene33873 | 0.86 | 2.80E-63 | -1.69E-46 |
| MS.gene050397 | MS.gene33873 | 0.872945 | 2.15E-67 | -1.69E-46 |
| MS.gene050404 | MS.gene33873 | 0.816146 | 6.43E-52 | -1.69E-46 |
| MS.gene050406 | MS.gene33873 | 0.832322 | 1.01E-55 | -1.69E-46 |
| MS.gene050432 | MS.gene33873 | 0.906848 | 9.57E-81 | -1.69E-46 |
| MS.gene050464 | MS.gene33873 | 0.830014 | 3.73E-55 | -1.69E-46 |
| MS.gene050529 | MS.gene33873 | 0.836678 | 8.13E-57 | -1.69E-46 |
| MS.gene05053 | MS.gene33873 | 0.800878 | 1.17E-48 | -1.69E-46 |
| MS.gene050593 | MS.gene33873 | 0.807754 | 4.33E-50 | -1.69E-46 |
| MS.gene050670 | MS.gene33873 | 0.820568 | 6.40E-53 | -1.69E-46 |
| MS.gene050767 | MS.gene33873 | 0.894244 | 2.96E-75 | -1.69E-46 |
| MS.gene050770 | MS.gene33873 | 0.895297 | 1.10E-75 | -1.69E-46 |
| MS.gene050772 | MS.gene33873 | 0.805314 | 1.42E-49 | -1.69E-46 |
| MS.gene050783 | MS.gene33873 | 0.813958 | 1.97E-51 | -1.69E-46 |
| MS.gene05101 | MS.gene33873 | 0.883937 | 2.94E-71 | -1.69E-46 |
| MS.gene05105 | MS.gene33873 | 0.857601 | 1.46E-62 | -1.69E-46 |
| MS.gene051228 | MS.gene33873 | 0.818458 | 1.94E-52 | -1.69E-46 |
| MS.gene051471 | MS.gene33873 | 0.844122 | 9.19E-59 | -1.69E-46 |
| MS.gene051582 | MS.gene33873 | 0.808839 | 2.54E-50 | -1.69E-46 |
| MS.gene051692 | MS.gene33873 | 0.821546 | 3.81E-53 | -1.69E-46 |
| MS.gene051877 | MS.gene33873 | 0.827229 | 1.76E-54 | -1.69E-46 |
| MS.gene052078 | MS.gene33873 | 0.873903 | 1.03E-67 | -1.69E-46 |
| MS.gene052178 | MS.gene33873 | 0.843573 | 1.29E-58 | -1.69E-46 |
| MS.gene052188 | MS.gene33873 | 0.817932 | 2.55E-52 | -1.69E-46 |
| MS.gene052253 | MS.gene33873 | 0.821848 | 3.25E-53 | -1.69E-46 |
| MS.gene052271 | MS.gene33873 | 0.816002 | 6.92E-52 | -1.69E-46 |
| MS.gene052287 | MS.gene33873 | 0.813288 | 2.76E-51 | -1.69E-46 |
| MS.gene052290 | MS.gene33873 | 0.834553 | 2.80E-56 | -1.69E-46 |
| MS.gene052293 | MS.gene33873 | 0.803614 | 3.21E-49 | -1.69E-46 |
| MS.gene052310 | MS.gene33873 | 0.862622 | 4.45E-64 | -1.69E-46 |
| MS.gene052322 | MS.gene33873 | 0.840234 | 9.83E-58 | -1.69E-46 |
| MS.gene052326 | MS.gene33873 | 0.839871 | 1.22E-57 | -1.69E-46 |
| MS.gene052340 | MS.gene33873 | 0.836112 | 1.13E-56 | -1.69E-46 |
| MS.gene052421 | MS.gene33873 | 0.811713 | 6.10E-51 | -1.69E-46 |
| MS.gene052481 | MS.gene33873 | 0.818179 | 2.24E-52 | -1.69E-46 |
| MS.gene052484 | MS.gene33873 | 0.821623 | 3.66E-53 | -1.69E-46 |
| MS.gene052485 | MS.gene33873 | 0.815632 | 8.37E-52 | -1.69E-46 |
| MS.gene052508 | MS.gene33873 | 0.834792 | 2.44E-56 | -1.69E-46 |
| MS.gene052510 | MS.gene33873 | 0.814765 | 1.30E-51 | -1.69E-46 |
| MS.gene053009 | MS.gene33873 | 0.817476 | 3.23E-52 | -1.69E-46 |
| MS.gene053103 | MS.gene33873 | 0.832779 | 7.78E-56 | -1.69E-46 |
| MS.gene053104 | MS.gene33873 | 0.837071 | 6.45E-57 | -1.69E-46 |
| MS.gene053207 | MS.gene33873 | 0.810668 | 1.03E-50 | -1.69E-46 |
| MS.gene053209 | MS.gene33873 | 0.910857 | 1.17E-82 | -1.69E-46 |
| MS.gene053322 | MS.gene33873 | 0.850666 | 1.47E-60 | -1.69E-46 |
| MS.gene053557 | MS.gene33873 | 0.823263 | 1.52E-53 | -1.69E-46 |
| MS.gene053813 | MS.gene33873 | 0.80415 | 2.48E-49 | -1.69E-46 |
| MS.gene053836 | MS.gene33873 | 0.834391 | 3.08E-56 | -1.69E-46 |
| MS.gene054123 | MS.gene33873 | 0.817619 | 3.00E-52 | -1.69E-46 |
| MS.gene054376 | MS.gene33873 | 0.908987 | 9.36E-82 | -1.69E-46 |
| MS.gene05455 | MS.gene33873 | 0.81317 | 2.93E-51 | -1.69E-46 |
| MS.gene054618 | MS.gene33873 | 0.800302 | 1.54E-48 | -1.69E-46 |
| MS.gene054802 | MS.gene33873 | 0.852105 | 5.74E-61 | -1.69E-46 |
| MS.gene054803 | MS.gene33873 | 0.849587 | 2.94E-60 | -1.69E-46 |
| MS.gene054804 | MS.gene33873 | 0.854634 | 1.08E-61 | -1.69E-46 |
| MS.gene05505 | MS.gene33873 | 0.809131 | 2.20E-50 | -1.69E-46 |
| MS.gene055116 | MS.gene33873 | 0.88682 | 2.45E-72 | -1.69E-46 |
| MS.gene055254 | MS.gene33873 | 0.886036 | 4.85E-72 | -1.69E-46 |
| MS.gene055277 | MS.gene33873 | 0.910361 | 2.04E-82 | -1.69E-46 |
| MS.gene055390 | MS.gene33873 | 0.845786 | 3.27E-59 | -1.69E-46 |
| MS.gene055397 | MS.gene33873 | 0.840829 | 6.87E-58 | -1.69E-46 |
| MS.gene055422 | MS.gene33873 | 0.856799 | 2.52E-62 | -1.69E-46 |
| MS.gene055428 | MS.gene33873 | 0.852295 | 5.07E-61 | -1.69E-46 |
| MS.gene055499 | MS.gene33873 | 0.862633 | 4.41E-64 | -1.69E-46 |
| MS.gene055564 | MS.gene33873 | 0.802341 | 5.88E-49 | -1.69E-46 |
| MS.gene055604 | MS.gene33873 | 0.807721 | 4.40E-50 | -1.69E-46 |
| MS.gene055647 | MS.gene33873 | 0.814432 | 1.54E-51 | -1.69E-46 |
| MS.gene055712 | MS.gene33873 | 0.832874 | 7.37E-56 | -1.69E-46 |
| MS.gene055722 | MS.gene33873 | 0.806393 | 8.41E-50 | -1.69E-46 |
| MS.gene055896 | MS.gene33873 | 0.805217 | 1.49E-49 | -1.69E-46 |
| MS.gene05592 | MS.gene33873 | 0.868619 | 5.71E-66 | -1.69E-46 |
| MS.gene056191 | MS.gene33873 | 0.873921 | 1.01E-67 | -1.69E-46 |
| MS.gene056374 | MS.gene33873 | 0.802091 | 6.61E-49 | -1.69E-46 |
| MS.gene056407 | MS.gene33873 | 0.85391 | 1.75E-61 | -1.69E-46 |
| MS.gene056410 | MS.gene33873 | 0.850952 | 1.22E-60 | -1.69E-46 |
| MS.gene056609 | MS.gene33873 | 0.860591 | 1.86E-63 | -1.69E-46 |
| MS.gene056612 | MS.gene33873 | 0.843653 | 1.23E-58 | -1.69E-46 |
| MS.gene056796 | MS.gene33873 | 0.809653 | 1.70E-50 | -1.69E-46 |
| MS.gene057011 | MS.gene33873 | 0.813577 | 2.39E-51 | -1.69E-46 |
| MS.gene05702 | MS.gene33873 | 0.880417 | 5.58E-70 | -1.69E-46 |
| MS.gene057123 | MS.gene33873 | 0.809043 | 2.30E-50 | -1.69E-46 |
| MS.gene057124 | MS.gene33873 | 0.826374 | 2.81E-54 | -1.69E-46 |
| MS.gene057129 | MS.gene33873 | 0.871072 | 9.03E-67 | -1.69E-46 |
| MS.gene057295 | MS.gene33873 | 0.861225 | 1.19E-63 | -1.69E-46 |
| MS.gene057331 | MS.gene33873 | 0.845106 | 4.99E-59 | -1.69E-46 |
| MS.gene05733 | MS.gene33873 | 0.825492 | 4.55E-54 | -1.69E-46 |
| MS.gene05735 | MS.gene33873 | 0.810381 | 1.19E-50 | -1.69E-46 |
| MS.gene057416 | MS.gene33873 | 0.820583 | 6.35E-53 | -1.69E-46 |
| MS.gene057439 | MS.gene33873 | 0.816847 | 4.48E-52 | -1.69E-46 |
| MS.gene057448 | MS.gene33873 | 0.817359 | 3.44E-52 | -1.69E-46 |
| MS.gene057449 | MS.gene33873 | 0.810754 | 9.85E-51 | -1.69E-46 |
| MS.gene057450 | MS.gene33873 | 0.827996 | 1.15E-54 | -1.69E-46 |
| MS.gene057796 | MS.gene33873 | 0.814197 | 1.74E-51 | -1.69E-46 |
| MS.gene058002 | MS.gene33873 | 0.836367 | 9.76E-57 | -1.69E-46 |
| MS.gene058006 | MS.gene33873 | 0.829972 | 3.82E-55 | -1.69E-46 |
| MS.gene058019 | MS.gene33873 | 0.820371 | 7.11E-53 | -1.69E-46 |
| MS.gene058082 | MS.gene33873 | 0.843756 | 1.15E-58 | -1.69E-46 |
| MS.gene05811 | MS.gene33873 | 0.822912 | 1.84E-53 | -1.69E-46 |
| MS.gene058233 | MS.gene33873 | 0.846767 | 1.77E-59 | -1.69E-46 |
| MS.gene058293 | MS.gene33873 | 0.876535 | 1.29E-68 | -1.69E-46 |
| MS.gene058323 | MS.gene33873 | 0.822914 | 1.84E-53 | -1.69E-46 |
| MS.gene058365 | MS.gene33873 | 0.823023 | 1.73E-53 | -1.69E-46 |
| MS.gene058660 | MS.gene33873 | 0.878636 | 2.39E-69 | -1.69E-46 |
| MS.gene058787 | MS.gene33873 | 0.839929 | 1.18E-57 | -1.69E-46 |
| MS.gene058928 | MS.gene33873 | 0.815454 | 9.17E-52 | -1.69E-46 |
| MS.gene05907 | MS.gene33873 | 0.837987 | 3.76E-57 | -1.69E-46 |
| MS.gene059228 | MS.gene33873 | 0.819844 | 9.38E-53 | -1.69E-46 |
| MS.gene059294 | MS.gene33873 | 0.814184 | 1.75E-51 | -1.69E-46 |
| MS.gene059326 | MS.gene33873 | 0.848805 | 4.85E-60 | -1.69E-46 |
| MS.gene059518 | MS.gene33873 | 0.83628 | 1.03E-56 | -1.69E-46 |
| MS.gene059536 | MS.gene33873 | 0.835687 | 1.45E-56 | -1.69E-46 |
| MS.gene059597 | MS.gene33873 | 0.824108 | 9.65E-54 | -1.69E-46 |
| MS.gene059676 | MS.gene33873 | 0.830308 | 3.16E-55 | -1.69E-46 |
| MS.gene059688 | MS.gene33873 | 0.88779 | 1.05E-72 | -1.69E-46 |
| MS.gene059692 | MS.gene33873 | 0.903458 | 3.40E-79 | -1.69E-46 |
| MS.gene059695 | MS.gene33873 | 0.899816 | 1.36E-77 | -1.69E-46 |
| MS.gene059708 | MS.gene33873 | 0.804565 | 2.03E-49 | -1.69E-46 |
| MS.gene059711 | MS.gene33873 | 0.801705 | 7.94E-49 | -1.69E-46 |
| MS.gene059894 | MS.gene33873 | 0.866116 | 3.61E-65 | -1.69E-46 |
| MS.gene059960 | MS.gene33873 | 0.845306 | 4.41E-59 | -1.69E-46 |
| MS.gene059974 | MS.gene33873 | 0.84505 | 5.17E-59 | -1.69E-46 |
| MS.gene060005 | MS.gene33873 | 0.830954 | 2.20E-55 | -1.69E-46 |
| MS.gene06037 | MS.gene33873 | 0.906386 | 1.57E-80 | -1.69E-46 |
| MS.gene060403 | MS.gene33873 | 0.845046 | 5.18E-59 | -1.69E-46 |
| MS.gene060804 | MS.gene33873 | 0.855616 | 5.60E-62 | -1.69E-46 |
| MS.gene060818 | MS.gene33873 | 0.813198 | 2.89E-51 | -1.69E-46 |
| MS.gene060837 | MS.gene33873 | 0.801225 | 9.96E-49 | -1.69E-46 |
| MS.gene061041 | MS.gene33873 | 0.822114 | 2.82E-53 | -1.69E-46 |
| MS.gene061049 | MS.gene33873 | 0.886852 | 2.38E-72 | -1.69E-46 |
| MS.gene061051 | MS.gene33873 | -0.853984 | 1.66E-61 | -1.69E-46 |
| MS.gene061112 | MS.gene33873 | 0.812275 | 4.60E-51 | -1.69E-46 |
| MS.gene061154 | MS.gene33873 | 0.801905 | 7.22E-49 | -1.69E-46 |
| MS.gene061200 | MS.gene33873 | 0.843443 | 1.40E-58 | -1.69E-46 |
| MS.gene061265 | MS.gene33873 | 0.803326 | 3.68E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene33873.t1 | MTR_1g026820 | 96.923 | 130 | 4 | 0 | 1 | 130 | 1 | 130 | 2.01e-87 | 250 |
| MS.gene33873.t1 | MTR_1g026820 | 98.980 | 98 | 1 | 0 | 33 | 130 | 1 | 98 | 4.30e-65 | 192 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene33873.t1 | AT1G30380 | 81.679 | 131 | 22 | 2 | 1 | 130 | 1 | 130 | 5.92e-73 | 214 |
Find 53 sgRNAs with CRISPR-Local
Find 69 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATTGGTGTTGGTGTTGTTCT+TGG | 0.264103 | 1.2:-12891259 | MS.gene33873:CDS |
| GCGCAAGGGCAAGGGTGCTT+TGG | 0.287491 | 1.2:-12891782 | MS.gene33873:CDS |
| ATCTTCCAGCAAACAACATT+AGG | 0.298751 | 1.2:+12891419 | None:intergenic |
| TTGCCCTTGCGCCTTGTCAT+AGG | 0.312155 | 1.2:+12891793 | None:intergenic |
| CGCAAGGGCAAGGGTGCTTT+GGG | 0.326430 | 1.2:-12891781 | MS.gene33873:CDS |
| GTTCACCCTTGCTGATACTT+TGG | 0.359682 | 1.2:-12891305 | MS.gene33873:CDS |
| TTGCAGACTGGTGACCCAGC+TGG | 0.370604 | 1.2:-12891328 | MS.gene33873:CDS |
| ACCTTCTCCTATCCAGGGTT+TGG | 0.377236 | 1.2:-12892093 | MS.gene33873:intron |
| ATGTTGTTTGCTGGAAGATT+TGG | 0.379579 | 1.2:-12891415 | MS.gene33873:CDS |
| TTGAAATCATACCACATTAG+TGG | 0.382365 | 1.2:+12891732 | None:intergenic |
| GGAGTTCGTTGTGACTTCAT+TGG | 0.392047 | 1.2:-12891760 | MS.gene33873:CDS |
| TCTTCCAGCAAACAACATTA+GGG | 0.393888 | 1.2:+12891420 | None:intergenic |
| ACAACCCTAATGTTGTTTGC+TGG | 0.398888 | 1.2:-12891424 | MS.gene33873:CDS |
| CTTGCGCCTTGTCATAGGTT+GGG | 0.405272 | 1.2:+12891798 | None:intergenic |
| CCTTGCGCCTTGTCATAGGT+TGG | 0.408508 | 1.2:+12891797 | None:intergenic |
| TGCAGACTGGTGACCCAGCT+GGG | 0.430536 | 1.2:-12891327 | MS.gene33873:CDS |
| ACTCAAACCTTCTCCTATCC+AGG | 0.437202 | 1.2:-12892099 | MS.gene33873:CDS |
| TCAGCAAGGGTGAACCCAGC+TGG | 0.438597 | 1.2:+12891313 | None:intergenic |
| GATTGGCTCCCTCAGCAAAC+AGG | 0.449260 | 1.2:-12891393 | MS.gene33873:CDS |
| ACTTTGGCCTGTGGGACTGT+TGG | 0.461540 | 1.2:-12891289 | MS.gene33873:CDS |
| ACAACTTTGCCTCAATTCAC+TGG | 0.462189 | 1.2:-12892134 | MS.gene33873:CDS |
| AGCAACAGCAGGACTAAAGC+TGG | 0.487183 | 1.2:-12891368 | MS.gene33873:CDS |
| GTTGCTTTCCTGTTTGCTGA+GGG | 0.491197 | 1.2:+12891385 | None:intergenic |
| TATGACAAGGCGCAAGGGCA+AGG | 0.493251 | 1.2:-12891791 | MS.gene33873:CDS |
| GTTTGCTGGAAGATTTGGAT+TGG | 0.494741 | 1.2:-12891410 | MS.gene33873:CDS |
| TTGCTGATACTTTGGCCTGT+GGG | 0.511025 | 1.2:-12891297 | MS.gene33873:CDS |
| CTTGCTGATACTTTGGCCTG+TGG | 0.513317 | 1.2:-12891298 | MS.gene33873:CDS |
| GGGACTGTTGGACACATCAT+TGG | 0.518734 | 1.2:-12891277 | MS.gene33873:CDS |
| GTTCTTGGTCTTAAGAACAT+TGG | 0.536798 | 1.2:-12891244 | MS.gene33873:CDS |
| AGGGACTCAGGCTTGCAGAC+TGG | 0.536809 | 1.2:-12891340 | MS.gene33873:CDS |
| CAGGACTAAAGCTGGAGACA+AGG | 0.539368 | 1.2:-12891360 | MS.gene33873:CDS |
| ATGACAAGGCGCAAGGGCAA+GGG | 0.543094 | 1.2:-12891790 | MS.gene33873:CDS |
| GGTTTAAGACCAGTGAATTG+AGG | 0.543474 | 1.2:+12892125 | None:intergenic |
| ATAGGAGAAGGTTTGAGTTG+TGG | 0.551141 | 1.2:+12892104 | None:intergenic |
| TGTTGCTTTCCTGTTTGCTG+AGG | 0.553786 | 1.2:+12891384 | None:intergenic |
| ACCAAACCCTGGATAGGAGA+AGG | 0.553964 | 1.2:+12892092 | None:intergenic |
| CCAACCTATGACAAGGCGCA+AGG | 0.557141 | 1.2:-12891797 | MS.gene33873:CDS |
| GTTGGACACATCATTGGTGT+TGG | 0.557734 | 1.2:-12891271 | MS.gene33873:CDS |
| AAGCTGGAGACAAGGGACTC+AGG | 0.559360 | 1.2:-12891352 | MS.gene33873:CDS |
| GATGTGTCCAACAGTCCCAC+AGG | 0.566507 | 1.2:+12891282 | None:intergenic |
| CAGCAAGGGTGAACCCAGCT+GGG | 0.567280 | 1.2:+12891314 | None:intergenic |
| AGGCAAAGTTGTCATCATAG+AGG | 0.584160 | 1.2:+12892145 | None:intergenic |
| TTAATTACCAAACCCTGGAT+AGG | 0.584959 | 1.2:+12892086 | None:intergenic |
| CATTGGTTCATCCACTAATG+TGG | 0.586749 | 1.2:-12891743 | MS.gene33873:intron |
| CAACCTATGACAAGGCGCAA+GGG | 0.587502 | 1.2:-12891796 | MS.gene33873:CDS |
| GGTGGTTAATTACCAAACCC+TGG | 0.592935 | 1.2:+12892081 | None:intergenic |
| AGGACTAAAGCTGGAGACAA+GGG | 0.595838 | 1.2:-12891359 | MS.gene33873:CDS |
| CTCAAACCTTCTCCTATCCA+GGG | 0.595941 | 1.2:-12892098 | MS.gene33873:CDS |
| CACAGGCCAAAGTATCAGCA+AGG | 0.601389 | 1.2:+12891299 | None:intergenic |
| GCAAACAGGAAAGCAACAGC+AGG | 0.611764 | 1.2:-12891379 | MS.gene33873:CDS |
| ACAGGCCAAAGTATCAGCAA+GGG | 0.672894 | 1.2:+12891300 | None:intergenic |
| TTGCTGCCCAACCTATGACA+AGG | 0.674324 | 1.2:-12891804 | MS.gene33873:CDS |
| ACATGTTGGACAGATTATGG+TGG | 0.710941 | 1.2:-12891452 | MS.gene33873:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTAAGTTTTATAATTCTTA+AGG | + | chr1.2:12891467-12891486 | None:intergenic | 10.0% |
| !! | AAAACTATACATATCATGAT+TGG | + | chr1.2:12891839-12891858 | None:intergenic | 20.0% |
| !! | ATGAATAAAAACATTGGAAT+TGG | + | chr1.2:12891695-12891714 | None:intergenic | 20.0% |
| !! | TATTCTATATACTTACTTCT+TGG | - | chr1.2:12891409-12891428 | MS.gene33873:CDS | 20.0% |
| !!! | TGTGTTTTTTTTTTATGTGA+AGG | - | chr1.2:12891559-12891578 | MS.gene33873:intron | 20.0% |
| ! | AAGTTACAAATTCTAACAAG+TGG | + | chr1.2:12891891-12891910 | None:intergenic | 25.0% |
| ! | ACAAAATGATGACATGAAAA+AGG | + | chr1.2:12891329-12891348 | None:intergenic | 25.0% |
| ! | CACACTATGAATAAAAACAT+TGG | + | chr1.2:12891701-12891720 | None:intergenic | 25.0% |
| AAAAACATTGGAATTGGAGT+TGG | + | chr1.2:12891689-12891708 | None:intergenic | 30.0% | |
| AAATGATGACATGAAAAAGG+TGG | + | chr1.2:12891326-12891345 | None:intergenic | 30.0% | |
| TAACGAGTTTCGCAAAATTA+CGG | + | chr1.2:12891814-12891833 | None:intergenic | 30.0% | |
| TCATAGTGTGTTTGAATTGA+CGG | - | chr1.2:12891711-12891730 | MS.gene33873:intron | 30.0% | |
| TTGAAATCATACCACATTAG+TGG | + | chr1.2:12891657-12891676 | None:intergenic | 30.0% | |
| ! | TATGATTGACTTTGACATGT+TGG | - | chr1.2:12891920-12891939 | MS.gene33873:intron | 30.0% |
| ATCTTCCAGCAAACAACATT+AGG | + | chr1.2:12891970-12891989 | None:intergenic | 35.0% | |
| TCTTCCAGCAAACAACATTA+GGG | + | chr1.2:12891969-12891988 | None:intergenic | 35.0% | |
| TTAATTACCAAACCCTGGAT+AGG | + | chr1.2:12891303-12891322 | None:intergenic | 35.0% | |
| TTGACATGTTGGACAGATTA+TGG | - | chr1.2:12891931-12891950 | MS.gene33873:intron | 35.0% | |
| ! | ATGTTGTTTGCTGGAAGATT+TGG | - | chr1.2:12891971-12891990 | MS.gene33873:intron | 35.0% |
| !! | GTTCTTGGTCTTAAGAACAT+TGG | - | chr1.2:12892142-12892161 | MS.gene33873:CDS | 35.0% |
| ACAACTTTGCCTCAATTCAC+TGG | - | chr1.2:12891252-12891271 | MS.gene33873:CDS | 40.0% | |
| ACATGTTGGACAGATTATGG+TGG | - | chr1.2:12891934-12891953 | MS.gene33873:intron | 40.0% | |
| AGGCAAAGTTGTCATCATAG+AGG | + | chr1.2:12891244-12891263 | None:intergenic | 40.0% | |
| ! | ACAACCCTAATGTTGTTTGC+TGG | - | chr1.2:12891962-12891981 | MS.gene33873:intron | 40.0% |
| ! | GGTTTAAGACCAGTGAATTG+AGG | + | chr1.2:12891264-12891283 | None:intergenic | 40.0% |
| ! | GTTTGCTGGAAGATTTGGAT+TGG | - | chr1.2:12891976-12891995 | MS.gene33873:intron | 40.0% |
| !! | ATAGGAGAAGGTTTGAGTTG+TGG | + | chr1.2:12891285-12891304 | None:intergenic | 40.0% |
| !! | ATTGGTGTTGGTGTTGTTCT+TGG | - | chr1.2:12892127-12892146 | MS.gene33873:CDS | 40.0% |
| !! | CATTGGTTCATCCACTAATG+TGG | - | chr1.2:12891643-12891662 | MS.gene33873:intron | 40.0% |
| ACTCAAACCTTCTCCTATCC+AGG | - | chr1.2:12891287-12891306 | MS.gene33873:CDS | 45.0% | |
| AGGACTAAAGCTGGAGACAA+GGG | - | chr1.2:12892027-12892046 | MS.gene33873:intron | 45.0% | |
| CTCAAACCTTCTCCTATCCA+GGG | - | chr1.2:12891288-12891307 | MS.gene33873:CDS | 45.0% | |
| GGAGTTCGTTGTGACTTCAT+TGG | - | chr1.2:12891626-12891645 | MS.gene33873:intron | 45.0% | |
| GGTGGTTAATTACCAAACCC+TGG | + | chr1.2:12891308-12891327 | None:intergenic | 45.0% | |
| GTTCACCCTTGCTGATACTT+TGG | - | chr1.2:12892081-12892100 | MS.gene33873:intron | 45.0% | |
| GTTGGACACATCATTGGTGT+TGG | - | chr1.2:12892115-12892134 | MS.gene33873:CDS | 45.0% | |
| TTGCTGATACTTTGGCCTGT+GGG | - | chr1.2:12892089-12892108 | MS.gene33873:intron | 45.0% | |
| ! | ACAGGCCAAAGTATCAGCAA+GGG | + | chr1.2:12892089-12892108 | None:intergenic | 45.0% |
| ! | GTTGCTTTCCTGTTTGCTGA+GGG | + | chr1.2:12892004-12892023 | None:intergenic | 45.0% |
| !! | TGTTGCTTTCCTGTTTGCTG+AGG | + | chr1.2:12892005-12892024 | None:intergenic | 45.0% |
| !! | AATTTAAATATATATTATGA+AGG | + | chr1.2:12891503-12891522 | None:intergenic | 5.0% |
| !!! | CATAATATATATTTAAATTT+TGG | - | chr1.2:12891504-12891523 | MS.gene33873:intron | 5.0% |
| ACCAAACCCTGGATAGGAGA+AGG | + | chr1.2:12891297-12891316 | None:intergenic | 50.0% | |
| ACCTTCTCCTATCCAGGGTT+TGG | - | chr1.2:12891293-12891312 | MS.gene33873:CDS | 50.0% | |
| AGCAACAGCAGGACTAAAGC+TGG | - | chr1.2:12892018-12892037 | MS.gene33873:intron | 50.0% | |
| CAACCTATGACAAGGCGCAA+GGG | - | chr1.2:12891590-12891609 | MS.gene33873:intron | 50.0% | |
| CAGGACTAAAGCTGGAGACA+AGG | - | chr1.2:12892026-12892045 | MS.gene33873:intron | 50.0% | |
| CTTGCGCCTTGTCATAGGTT+GGG | + | chr1.2:12891591-12891610 | None:intergenic | 50.0% | |
| CTTGCTGATACTTTGGCCTG+TGG | - | chr1.2:12892088-12892107 | MS.gene33873:intron | 50.0% | |
| GCAAACAGGAAAGCAACAGC+AGG | - | chr1.2:12892007-12892026 | MS.gene33873:intron | 50.0% | |
| GGGACTGTTGGACACATCAT+TGG | - | chr1.2:12892109-12892128 | MS.gene33873:CDS | 50.0% | |
| TTGCTGCCCAACCTATGACA+AGG | - | chr1.2:12891582-12891601 | MS.gene33873:intron | 50.0% | |
| ! | CACAGGCCAAAGTATCAGCA+AGG | + | chr1.2:12892090-12892109 | None:intergenic | 50.0% |
| AAGCTGGAGACAAGGGACTC+AGG | - | chr1.2:12892034-12892053 | MS.gene33873:intron | 55.0% | |
| ACTTTGGCCTGTGGGACTGT+TGG | - | chr1.2:12892097-12892116 | MS.gene33873:CDS | 55.0% | |
| ATGACAAGGCGCAAGGGCAA+GGG | - | chr1.2:12891596-12891615 | MS.gene33873:intron | 55.0% | |
| CCAACCTATGACAAGGCGCA+AGG | - | chr1.2:12891589-12891608 | MS.gene33873:intron | 55.0% | |
| CCTTGCGCCTTGTCATAGGT+TGG | + | chr1.2:12891592-12891611 | None:intergenic | 55.0% | |
| GATGTGTCCAACAGTCCCAC+AGG | + | chr1.2:12892107-12892126 | None:intergenic | 55.0% | |
| TATGACAAGGCGCAAGGGCA+AGG | - | chr1.2:12891595-12891614 | MS.gene33873:intron | 55.0% | |
| TTGCCCTTGCGCCTTGTCAT+AGG | + | chr1.2:12891596-12891615 | None:intergenic | 55.0% | |
| ! | GATTGGCTCCCTCAGCAAAC+AGG | - | chr1.2:12891993-12892012 | MS.gene33873:intron | 55.0% |
| AGGGACTCAGGCTTGCAGAC+TGG | - | chr1.2:12892046-12892065 | MS.gene33873:intron | 60.0% | |
| CAGCAAGGGTGAACCCAGCT+GGG | + | chr1.2:12892075-12892094 | None:intergenic | 60.0% | |
| TCAGCAAGGGTGAACCCAGC+TGG | + | chr1.2:12892076-12892095 | None:intergenic | 60.0% | |
| TGCAGACTGGTGACCCAGCT+GGG | - | chr1.2:12892059-12892078 | MS.gene33873:intron | 60.0% | |
| TTGCAGACTGGTGACCCAGC+TGG | - | chr1.2:12892058-12892077 | MS.gene33873:intron | 60.0% | |
| !! | CGCAAGGGCAAGGGTGCTTT+GGG | - | chr1.2:12891605-12891624 | MS.gene33873:intron | 60.0% |
| !! | GCGCAAGGGCAAGGGTGCTT+TGG | - | chr1.2:12891604-12891623 | MS.gene33873:intron | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.2 | gene | 12891234 | 12892174 | 12891234 | ID=MS.gene33873 |
| chr1.2 | mRNA | 12891234 | 12892174 | 12891234 | ID=MS.gene33873.t1;Parent=MS.gene33873 |
| chr1.2 | exon | 12892094 | 12892174 | 12892094 | ID=MS.gene33873.t1.exon1;Parent=MS.gene33873.t1 |
| chr1.2 | CDS | 12892094 | 12892174 | 12892094 | ID=cds.MS.gene33873.t1;Parent=MS.gene33873.t1 |
| chr1.2 | exon | 12891744 | 12891827 | 12891744 | ID=MS.gene33873.t1.exon2;Parent=MS.gene33873.t1 |
| chr1.2 | CDS | 12891744 | 12891827 | 12891744 | ID=cds.MS.gene33873.t1;Parent=MS.gene33873.t1 |
| chr1.2 | exon | 12891234 | 12891461 | 12891234 | ID=MS.gene33873.t1.exon3;Parent=MS.gene33873.t1 |
| chr1.2 | CDS | 12891234 | 12891461 | 12891234 | ID=cds.MS.gene33873.t1;Parent=MS.gene33873.t1 |
| Gene Sequence |
| Protein sequence |
