AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene072571


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene072571.t1 MTR_7g111880 96.000 250 9 1 1 249 1 250 3.30e-172 474
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene072571.t1 AT4G15510 64.659 249 76 5 10 247 40 287 4.09e-105 306
MS.gene072571.t1 AT4G15510 73.039 204 52 2 46 247 3 205 2.02e-103 298
MS.gene072571.t1 AT4G15510 73.039 204 52 2 46 247 3 205 2.02e-103 298
MS.gene072571.t1 AT4G15510 73.039 204 52 2 46 247 3 205 2.02e-103 298
MS.gene072571.t1 AT4G15510 69.595 148 40 3 35 178 72 218 1.67e-62 196

Find 0 sgRNAs with CRISPR-Local

Find 70 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATTCTTGATGAAAATATAT+CGG + 51773:3763-3782 MS.gene072571:CDS 15.0%
!!! ATATTTTCATCAAGAATATA+AGG - 51773:3761-3780 None:intergenic 15.0%
!! AAATAAAATAATTGTGACAG+AGG - 51773:3489-3508 None:intergenic 20.0%
!! TACATTTATCAGAAAAATTG+TGG + 51773:3990-4009 MS.gene072571:intron 20.0%
! GAATATGTTGATACATTTGA+TGG + 51773:3665-3684 MS.gene072571:CDS 25.0%
AAAACTTACGGTAATTTGAG+GGG + 51773:4038-4057 MS.gene072571:intron 30.0%
AGAAAATGTGTTTCAAGAAG+AGG + 51773:4307-4326 MS.gene072571:CDS 30.0%
CAAAACTTACGGTAATTTGA+GGG + 51773:4037-4056 MS.gene072571:intron 30.0%
CATTCAACTATCCATCAAAT+TGG + 51773:3693-3712 MS.gene072571:CDS 30.0%
CTTACTTGAATCCAATTTGA+TGG - 51773:3707-3726 None:intergenic 30.0%
TCAAATTGGATTCAAGTAAG+AGG + 51773:3707-3726 MS.gene072571:CDS 30.0%
! CAAATTGTACTATCAAGTTG+AGG + 51773:3961-3980 MS.gene072571:CDS 30.0%
! TCTTCTTGAAACACATTTTC+TGG - 51773:4308-4327 None:intergenic 30.0%
!! AATGACATTGCTTTTCTTCT+AGG - 51773:3578-3597 None:intergenic 30.0%
ATGGATGGAATTTACCGAAT+TGG - 51773:3452-3471 None:intergenic 35.0%
ATTCAAGACTGTTCAAGACT+TGG + 51773:3814-3833 MS.gene072571:CDS 35.0%
CTCTGTTCTTGCAAAACTTA+CGG + 51773:4026-4045 MS.gene072571:intron 35.0%
GCAAAACTTACGGTAATTTG+AGG + 51773:4036-4055 MS.gene072571:intron 35.0%
GTAATCTCAACTCATACAGT+TGG - 51773:4280-4299 None:intergenic 35.0%
GTCTTGAATTTCGAAGAAGA+AGG - 51773:3803-3822 None:intergenic 35.0%
TGGATGGAATTTACCGAATT+GGG - 51773:3451-3470 None:intergenic 35.0%
TTACTGAGTTTATGTCCACT+AGG + 51773:3879-3898 MS.gene072571:CDS 35.0%
TTCAAGACTGTTCAAGACTT+GGG + 51773:3815-3834 MS.gene072571:CDS 35.0%
!!! TGGTGCTGATATTTTCTTCA+GGG + 51773:3733-3752 MS.gene072571:CDS 35.0%
!!! TTTGAGGGGTGTCTTTTATA+TGG + 51773:4052-4071 MS.gene072571:intron 35.0%
ACATATTCACGAAACACACG+AGG - 51773:3653-3672 None:intergenic 40.0%
ACCCGTTGTTGTGATTGTAT+TGG - 51773:3365-3384 None:intergenic 40.0%
ACTTACATTCTCTCCGACAT+TGG + 51773:3608-3627 MS.gene072571:CDS 40.0%
AGAGGAGAAAGCTCATGAAA+TGG - 51773:3471-3490 None:intergenic 40.0%
ATGGAAGTGAAATGGTGACA+TGG - 51773:3401-3420 None:intergenic 40.0%
GAATTTACCGAATTGGGTGT+GGG - 51773:3445-3464 None:intergenic 40.0%
GAGAAAGCTCATGAAATGGA+TGG - 51773:3467-3486 None:intergenic 40.0%
GCCAATACAATCACAACAAC+GGG + 51773:3361-3380 MS.gene072571:CDS 40.0%
GGCCAATACAATCACAACAA+CGG + 51773:3360-3379 MS.gene072571:CDS 40.0%
TGCTAATGCTGTACCAATGT+CGG - 51773:3624-3643 None:intergenic 40.0%
TGCTGTTATGCCACAAGATA+GGG + 51773:4199-4218 MS.gene072571:CDS 40.0%
TGGAAGTGAAATGGTGACAT+GGG - 51773:3400-3419 None:intergenic 40.0%
TTTCAACTCCTAGAACAGAC+AGG - 51773:4253-4272 None:intergenic 40.0%
! TTGCTGTTATGCCACAAGAT+AGG + 51773:4198-4217 MS.gene072571:CDS 40.0%
!! TGTGTGTTGTTTGTGTTGCT+AGG + 51773:4142-4161 MS.gene072571:intron 40.0%
!!! CTGGTGCTGATATTTTCTTC+AGG + 51773:3732-3751 MS.gene072571:CDS 40.0%
ACGTTTAACACCAAGCCTAG+TGG - 51773:3897-3916 None:intergenic 45.0%
AGTGAAATGGTGACATGGGT+CGG - 51773:3396-3415 None:intergenic 45.0%
ATCATCAGCAACTCTCTCAG+AGG - 51773:3939-3958 None:intergenic 45.0%
CATTTCTCCCACACCCAATT+CGG + 51773:3435-3454 MS.gene072571:CDS 45.0%
GAGTGAACTTCGTCAAGTCA+TGG + 51773:4331-4350 MS.gene072571:CDS 45.0%
GAGTTTATGTCCACTAGGCT+TGG + 51773:3884-3903 MS.gene072571:CDS 45.0%
GGAATTTACCGAATTGGGTG+TGG - 51773:3446-3465 None:intergenic 45.0%
GGCTTCGTTTCGAGCAAATA+AGG + 51773:4352-4371 MS.gene072571:CDS 45.0%
TCTGAGAGAGTTGCTGATGA+TGG + 51773:3938-3957 MS.gene072571:CDS 45.0%
TGTTATGCCACAAGATAGGG+TGG + 51773:4202-4221 MS.gene072571:CDS 45.0%
! TGGATTCAAGTAAGAGGTGC+TGG + 51773:3713-3732 MS.gene072571:CDS 45.0%
!! ACTTTCTTCCCAGCTTGTTG+AGG - 51773:3845-3864 None:intergenic 45.0%
AAACGCACCACCCTATCTTG+TGG - 51773:4212-4231 None:intergenic 50.0%
ACAAGATAGGGTGGTGCGTT+TGG + 51773:4211-4230 MS.gene072571:CDS 50.0%
ATAGGGTGGTGCGTTTGGAA+TGG + 51773:4216-4235 MS.gene072571:CDS 50.0%
ATCAGCAACTCTCTCAGAGG+TGG - 51773:3936-3955 None:intergenic 50.0%
CGAATTGGGTGTGGGAGAAA+TGG - 51773:3437-3456 None:intergenic 50.0%
CGAGGGGAATGGAAGTGAAA+TGG - 51773:3409-3428 None:intergenic 50.0%
GTGAAATGGTGACATGGGTC+GGG - 51773:3395-3414 None:intergenic 50.0%
! TAGGGTGGTGCGTTTGGAAT+GGG + 51773:4217-4236 MS.gene072571:CDS 50.0%
! TGCGTTTGGAATGGGATAGG+AGG + 51773:4225-4244 MS.gene072571:CDS 50.0%
! TGGTGCGTTTGGAATGGGAT+AGG + 51773:4222-4241 MS.gene072571:CDS 50.0%
!! AGGAGGTACCTGTCTGTTCT+AGG + 51773:4242-4261 MS.gene072571:CDS 50.0%
AAATGGAGCAGCGAGGGGAA+TGG - 51773:3420-3439 None:intergenic 55.0%
TGGGAGAAATGGAGCAGCGA+GGG - 51773:3426-3445 None:intergenic 55.0%
TGGGTTCGCCTCAACAAGCT+GGG + 51773:3834-3853 MS.gene072571:CDS 55.0%
TTGGGTTCGCCTCAACAAGC+TGG + 51773:3833-3852 MS.gene072571:CDS 55.0%
GGGAGAAATGGAGCAGCGAG+GGG - 51773:3425-3444 None:intergenic 60.0%
GTGGGAGAAATGGAGCAGCG+AGG - 51773:3427-3446 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
51773 gene 3358 4385 3358 ID=MS.gene072571
51773 mRNA 3358 4385 3358 ID=MS.gene072571.t1;Parent=MS.gene072571
51773 exon 3358 3456 3358 ID=MS.gene072571.t1.exon1;Parent=MS.gene072571.t1
51773 CDS 3358 3456 3358 ID=cds.MS.gene072571.t1;Parent=MS.gene072571.t1
51773 exon 3554 3982 3554 ID=MS.gene072571.t1.exon2;Parent=MS.gene072571.t1
51773 CDS 3554 3982 3554 ID=cds.MS.gene072571.t1;Parent=MS.gene072571.t1
51773 exon 4164 4385 4164 ID=MS.gene072571.t1.exon3;Parent=MS.gene072571.t1
51773 CDS 4164 4385 4164 ID=cds.MS.gene072571.t1;Parent=MS.gene072571.t1
Gene Sequence

>MS.gene072571

ATGGCCAATACAATCACAACAACGGGTTTTCTAGCCCGACCCATGTCACCATTTCACTTCCATTCCCCTCGCTGCTCCATTTCTCCCACACCCAATTCGGTAAATTCCATCCATTTCATGAGCTTTCTCCTCTGTCACAATTATTTTATTTTATTTACTAAATCTTTGATTTTTGTCTCTGCTAATATATAATCAGATTAAAGCAGCTTTTGCAGTTCCTAGAAGAAAAGCAATGTCATTAATCTTATCAACTTACATTCTCTCCGACATTGGTACAGCATTAGCACAACAACCTCGTGTGTTTCGTGAATATGTTGATACATTTGATGGTTATTCATTCAACTATCCATCAAATTGGATTCAAGTAAGAGGTGCTGGTGCTGATATTTTCTTCAGGGATCCTTATATTCTTGATGAAAATATATCGGTCGAAGTTTCTTCGCCTTCTTCTTCGAAATTCAAGACTGTTCAAGACTTGGGTTCGCCTCAACAAGCTGGGAAGAAAGTTCTTGATCAGTATCTTACTGAGTTTATGTCCACTAGGCTTGGTGTTAAACGTGAATCTAATATTCTTTCCACCTCTGAGAGAGTTGCTGATGATGGCAAATTGTACTATCAAGTTGAGGTTCGTTTACATTTATCAGAAAAATTGTGGTTAATCACGTTAACTCTGTTCTTGCAAAACTTACGGTAATTTGAGGGGTGTCTTTTATATGGTAATGAAATCATACATTGTAGTCTAGCGCTACTTGTTGCAAAATGACATTAAATGATTGATTGATTGTGTGTGTTGTTTGTGTTGCTAGGCGAACATTAAGTCATATGCTAGTAACAACGAGCTTGCTGTTATGCCACAAGATAGGGTGGTGCGTTTGGAATGGGATAGGAGGTACCTGTCTGTTCTAGGAGTTGAAAACAACCAACTGTATGAGTTGAGATTACAAGTGCCAGAAAATGTGTTTCAAGAAGAGGAGAGTGAACTTCGTCAAGTCATGGCTTCGTTTCGAGCAAATAAGGTTGTTCGTTAG

Protein sequence

>MS.gene072571.t1

MANTITTTGFLARPMSPFHFHSPRCSISPTPNSIKAAFAVPRRKAMSLILSTYILSDIGTALAQQPRVFREYVDTFDGYSFNYPSNWIQVRGAGADIFFRDPYILDENISVEVSSPSSSKFKTVQDLGSPQQAGKKVLDQYLTEFMSTRLGVKRESNILSTSERVADDGKLYYQVEANIKSYASNNELAVMPQDRVVRLEWDRRYLSVLGVENNQLYELRLQVPENVFQEEESELRQVMASFRANKVVR