AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene68129


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene68129.t1 MTR_3g118210 99.558 226 1 0 1 226 47 272 9.90e-165 455
MS.gene68129.t1 MTR_6g006970 42.718 206 114 4 12 216 49 251 9.85e-47 156
MS.gene68129.t1 MTR_1g093060 40.777 206 118 4 12 216 102 304 1.06e-42 147
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene68129.t1 AT2G43030 82.743 226 39 0 1 226 46 271 4.47e-130 368
MS.gene68129.t1 AT3G17465 37.037 216 133 3 11 225 77 290 2.47e-38 135

Find 78 sgRNAs with CRISPR-Local

Find 89 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTAACAATCTTAAGCTTTC+TGG 0.097361 3.1:-92911851 None:intergenic
AAAGAGACTAATTAATTCTT+TGG 0.209215 3.1:-92911976 None:intergenic
AAAGCTCATCATTCCCAATT+TGG 0.228676 3.1:-92911261 None:intergenic
CAAATTGACCAAACCTGAAA+TGG 0.282604 3.1:+92911418 MS.gene68129:CDS
CATTCCTATGCGACATCTTC+AGG 0.315485 3.1:+92911463 MS.gene68129:CDS
ATGATCAAAGGTGCTCTTCC+TGG 0.365904 3.1:+92911898 MS.gene68129:CDS
TTTCGTCTTCAATCCGTTGA+TGG 0.384156 3.1:+92911488 MS.gene68129:CDS
TCAAATACAAGGCGCTGATT+AGG 0.398930 3.1:-92911518 None:intergenic
AAGATGACCCATTTCAGGTT+TGG 0.399688 3.1:-92911426 None:intergenic
CTCTTGGAACCATGGCTCAT+TGG 0.403785 3.1:-92911730 None:intergenic
TGGTGTGATGGGTACCAAAT+TGG 0.414953 3.1:+92911247 MS.gene68129:CDS
GTTGTTCCCGTCACTGTTGT+CGG 0.415769 3.1:+92911302 MS.gene68129:CDS
GGTGATCTTGTTGATGTCTC+TGG 0.428399 3.1:+92911557 MS.gene68129:CDS
AAGCTTTCTGGTCTTCCTGT+TGG 0.434373 3.1:-92911839 None:intergenic
ACGCCGTCCAAGTTGGTTAC+CGG 0.438083 3.1:+92911381 MS.gene68129:CDS
TCCAAGTTGTCTGTGACTCT+TGG 0.442433 3.1:-92911746 None:intergenic
TCCAAGAGTCACAGACAACT+TGG 0.460086 3.1:+92911745 MS.gene68129:CDS
TTCTTGCCAACAATCTTAGC+GGG 0.460342 3.1:-92911949 None:intergenic
ACTGTTGTCGGTTTCAAAGA+AGG 0.461503 3.1:+92911314 MS.gene68129:CDS
AGAGGCTGGTGTTGGTGTGA+TGG 0.463975 3.1:+92911235 MS.gene68129:CDS
TACACCTGGTCGCGTATACA+AGG 0.469173 3.1:+92911786 MS.gene68129:CDS
TTTCAGGTTTGGTCAATTTG+CGG 0.469193 3.1:-92911415 None:intergenic
GTTGTAACCGTCGGTGGCGT+CGG 0.473671 3.1:-92911360 None:intergenic
AAAGAAGATGCCTGGTAGGA+TGG 0.474224 3.1:+92911810 MS.gene68129:CDS
GAGGCTGGTGTTGGTGTGAT+GGG 0.474712 3.1:+92911236 MS.gene68129:CDS
TAACCCTCCGGTAACCAACT+TGG 0.478957 3.1:-92911388 None:intergenic
CACAGACAACTTGGATCCAT+TGG 0.481197 3.1:+92911754 MS.gene68129:CDS
GTAGGATGGGTGGAACCAAC+AGG 0.493432 3.1:+92911824 MS.gene68129:CDS
TACGCGACCAGGTGTAGTTC+CGG 0.494636 3.1:-92911779 None:intergenic
TTTCCCTTGTATACGCGACC+AGG 0.499382 3.1:-92911790 None:intergenic
AGAAGATTACCAGGCTTACC+AGG 0.500896 3.1:-92911916 None:intergenic
TGAAATGGGTCATCTTGAAA+AGG 0.503372 3.1:+92911433 MS.gene68129:CDS
ACGGGAACAACTTCACCGTT+AGG 0.508452 3.1:-92911290 None:intergenic
AAGAAGATGCCTGGTAGGAT+GGG 0.509948 3.1:+92911811 MS.gene68129:CDS
GGTGTGATGGGTACCAAATT+GGG 0.512034 3.1:+92911248 MS.gene68129:CDS
AAATTGACCAAACCTGAAAT+GGG 0.513032 3.1:+92911419 MS.gene68129:CDS
TTGAAACCGACAACAGTGAC+GGG 0.513527 3.1:-92911308 None:intergenic
GGTGCTCTTCCTGGTAAGCC+TGG 0.514459 3.1:+92911907 MS.gene68129:CDS
AAGCGTCATAACTTCAAAAG+AGG 0.517261 3.1:+92911706 MS.gene68129:CDS
AGGTGTAGTTCCGGCACCAA+TGG 0.518535 3.1:-92911770 None:intergenic
TTGGTTCCACCCATCCTACC+AGG 0.519178 3.1:-92911820 None:intergenic
ATGGGTCATCTTGAAAAGGT+TGG 0.519826 3.1:+92911437 MS.gene68129:CDS
GATGTCTCTGGCACCACCAT+CGG 0.522268 3.1:+92911569 MS.gene68129:CDS
GGTGGTGAAAATGAGCTTAG+AGG 0.522460 3.1:+92911217 MS.gene68129:CDS
GTAAGCCTGGTAATCTTCTA+CGG 0.530779 3.1:+92911920 MS.gene68129:CDS
GAACAACTTCACCGTTAGGT+TGG 0.533148 3.1:-92911286 None:intergenic
CTCTGGCACCACCATCGGCA+AGG 0.534330 3.1:+92911574 MS.gene68129:CDS
TACAAGGGAAAGAAGATGCC+TGG 0.536322 3.1:+92911802 MS.gene68129:CDS
GTCTGTGACTCTTGGAACCA+TGG 0.543733 3.1:-92911738 None:intergenic
CTGAAGATGTCGCATAGGAA+TGG 0.560330 3.1:-92911462 None:intergenic
CAACTTGGATCCATTGGTGC+CGG 0.569215 3.1:+92911760 MS.gene68129:CDS
ATTTGCGGTCTCTAACCCTC+CGG 0.569415 3.1:-92911400 None:intergenic
ATTAGGTTCAAAACCATCAA+CGG 0.570807 3.1:-92911501 None:intergenic
TCTGGCACCACCATCGGCAA+GGG 0.572275 3.1:+92911575 MS.gene68129:CDS
CTTAATGTTATCATGATCAA+AGG 0.572918 3.1:+92911886 MS.gene68129:CDS
ATTGGTGCCGGAACTACACC+TGG 0.580799 3.1:+92911772 MS.gene68129:CDS
TTTGAAACCGACAACAGTGA+CGG 0.586413 3.1:-92911309 None:intergenic
CCGTCCAAGTTGGTTACCGG+AGG 0.591201 3.1:+92911384 MS.gene68129:CDS
GTTCTTGCCAACAATCTTAG+CGG 0.594511 3.1:-92911950 None:intergenic
AAGATGCCTGGTAGGATGGG+TGG 0.594546 3.1:+92911814 MS.gene68129:CDS
ACCTTGAAAACCCTTGCCGA+TGG 0.600155 3.1:-92911585 None:intergenic
TGAAAAGCTCATCAAATACA+AGG 0.600821 3.1:-92911529 None:intergenic
GCTATCCGTAGAAGATTACC+AGG 0.602366 3.1:-92911925 None:intergenic
AGGGAAAGAAGATGCCTGGT+AGG 0.605040 3.1:+92911806 MS.gene68129:CDS
TTGAAAACCCTTGCCGATGG+TGG 0.607518 3.1:-92911582 None:intergenic
ATCAAAACCGACGCCACCGA+CGG 0.609949 3.1:+92911353 MS.gene68129:CDS
GGTTACAACGCCGTCCAAGT+TGG 0.618166 3.1:+92911374 MS.gene68129:CDS
GTGAAAATGAGCTTAGAGGC+TGG 0.634928 3.1:+92911221 MS.gene68129:CDS
TCTTGCCAACAATCTTAGCG+GGG 0.638407 3.1:-92911948 None:intergenic
CCTCCGGTAACCAACTTGGA+CGG 0.639997 3.1:-92911384 None:intergenic
ATGAGCTTAGAGGCTGGTGT+TGG 0.640133 3.1:+92911227 MS.gene68129:CDS
AAAAGAGGACCAATGAGCCA+TGG 0.640937 3.1:+92911721 MS.gene68129:CDS
CGTCCAAGTTGGTTACCGGA+GGG 0.643249 3.1:+92911385 MS.gene68129:CDS
CTTGCCAACAATCTTAGCGG+GGG 0.643846 3.1:-92911947 None:intergenic
GACGGCGTTGTAACCGTCGG+TGG 0.648118 3.1:-92911366 None:intergenic
AACTCCTGAAGATGTCGCAT+AGG 0.655881 3.1:-92911467 None:intergenic
TTGGACGGCGTTGTAACCGT+CGG 0.663802 3.1:-92911369 None:intergenic
ACACCTGGTCGCGTATACAA+GGG 0.687415 3.1:+92911787 MS.gene68129:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAATAATATCTTTCACTAA+TGG + chr3.1:92911653-92911672 MS.gene68129:intron 15.0%
! CTTAATGTTATCATGATCAA+AGG + chr3.1:92911886-92911905 MS.gene68129:CDS 25.0%
! TTTAACAATCTTAAGCTTTC+TGG - chr3.1:92911854-92911873 None:intergenic 25.0%
AAATTGACCAAACCTGAAAT+GGG + chr3.1:92911419-92911438 MS.gene68129:CDS 30.0%
ATGAACTTACTTACTTTGAC+AGG + chr3.1:92911676-92911695 MS.gene68129:intron 30.0%
ATTAGGTTCAAAACCATCAA+CGG - chr3.1:92911504-92911523 None:intergenic 30.0%
TATCAACTCAACAACTAGAA+GGG - chr3.1:92911636-92911655 None:intergenic 30.0%
TGAAAAGCTCATCAAATACA+AGG - chr3.1:92911532-92911551 None:intergenic 30.0%
TTATCAACTCAACAACTAGA+AGG - chr3.1:92911637-92911656 None:intergenic 30.0%
! TTTGATGAGCTTTTCAATGA+AGG + chr3.1:92911536-92911555 MS.gene68129:CDS 30.0%
AAAGCTCATCATTCCCAATT+TGG - chr3.1:92911264-92911283 None:intergenic 35.0%
AACTTACTTACTTTGACAGG+TGG + chr3.1:92911679-92911698 MS.gene68129:intron 35.0%
AAGCGTCATAACTTCAAAAG+AGG + chr3.1:92911706-92911725 MS.gene68129:CDS 35.0%
CAAATTGACCAAACCTGAAA+TGG + chr3.1:92911418-92911437 MS.gene68129:CDS 35.0%
TGAAATGGGTCATCTTGAAA+AGG + chr3.1:92911433-92911452 MS.gene68129:CDS 35.0%
!! ATGAGCTTTTTCCAACCTAA+CGG + chr3.1:92911275-92911294 MS.gene68129:CDS 35.0%
!! TTTCAGGTTTGGTCAATTTG+CGG - chr3.1:92911418-92911437 None:intergenic 35.0%
!! TTTTCAAGATGACCCATTTC+AGG - chr3.1:92911434-92911453 None:intergenic 35.0%
AACTAGAAGGGAATGCAAAG+AGG - chr3.1:92911624-92911643 None:intergenic 40.0%
ACTAGAAGGGAATGCAAAGA+GGG - chr3.1:92911623-92911642 None:intergenic 40.0%
ATGGGTCATCTTGAAAAGGT+TGG + chr3.1:92911437-92911456 MS.gene68129:CDS 40.0%
GTAAGCCTGGTAATCTTCTA+CGG + chr3.1:92911920-92911939 MS.gene68129:CDS 40.0%
GTTCTTGCCAACAATCTTAG+CGG - chr3.1:92911953-92911972 None:intergenic 40.0%
TTCTTGCCAACAATCTTAGC+GGG - chr3.1:92911952-92911971 None:intergenic 40.0%
TTTCGTCTTCAATCCGTTGA+TGG + chr3.1:92911488-92911507 MS.gene68129:CDS 40.0%
! TTTGAAACCGACAACAGTGA+CGG - chr3.1:92911312-92911331 None:intergenic 40.0%
!! AAGATGACCCATTTCAGGTT+TGG - chr3.1:92911429-92911448 None:intergenic 40.0%
!! ACTGTTGTCGGTTTCAAAGA+AGG + chr3.1:92911314-92911333 MS.gene68129:CDS 40.0%
!! TCAAATACAAGGCGCTGATT+AGG - chr3.1:92911521-92911540 None:intergenic 40.0%
AAAAGAGGACCAATGAGCCA+TGG + chr3.1:92911721-92911740 MS.gene68129:CDS 45.0%
AAAGAAGATGCCTGGTAGGA+TGG + chr3.1:92911810-92911829 MS.gene68129:CDS 45.0%
AACTCCTGAAGATGTCGCAT+AGG - chr3.1:92911470-92911489 None:intergenic 45.0%
AAGAAGATGCCTGGTAGGAT+GGG + chr3.1:92911811-92911830 MS.gene68129:CDS 45.0%
AGAAGATTACCAGGCTTACC+AGG - chr3.1:92911919-92911938 None:intergenic 45.0%
CACAGACAACTTGGATCCAT+TGG + chr3.1:92911754-92911773 MS.gene68129:CDS 45.0%
CATTCCTATGCGACATCTTC+AGG + chr3.1:92911463-92911482 MS.gene68129:CDS 45.0%
CTGAAGATGTCGCATAGGAA+TGG - chr3.1:92911465-92911484 None:intergenic 45.0%
GAACAACTTCACCGTTAGGT+TGG - chr3.1:92911289-92911308 None:intergenic 45.0%
GCTATCCGTAGAAGATTACC+AGG - chr3.1:92911928-92911947 None:intergenic 45.0%
GGTGATCTTGTTGATGTCTC+TGG + chr3.1:92911557-92911576 MS.gene68129:CDS 45.0%
GGTGGTGAAAATGAGCTTAG+AGG + chr3.1:92911217-92911236 MS.gene68129:CDS 45.0%
GTGAAAATGAGCTTAGAGGC+TGG + chr3.1:92911221-92911240 MS.gene68129:CDS 45.0%
TACAAGGGAAAGAAGATGCC+TGG + chr3.1:92911802-92911821 MS.gene68129:CDS 45.0%
TCCAAGAGTCACAGACAACT+TGG + chr3.1:92911745-92911764 MS.gene68129:CDS 45.0%
TCCAAGTTGTCTGTGACTCT+TGG - chr3.1:92911749-92911768 None:intergenic 45.0%
TCTTGCCAACAATCTTAGCG+GGG - chr3.1:92911951-92911970 None:intergenic 45.0%
TTGAAACCGACAACAGTGAC+GGG - chr3.1:92911311-92911330 None:intergenic 45.0%
! AAGCTTTCTGGTCTTCCTGT+TGG - chr3.1:92911842-92911861 None:intergenic 45.0%
!! ATGATCAAAGGTGCTCTTCC+TGG + chr3.1:92911898-92911917 MS.gene68129:CDS 45.0%
!! GGTGTGATGGGTACCAAATT+GGG + chr3.1:92911248-92911267 MS.gene68129:CDS 45.0%
!! TGGTGTGATGGGTACCAAAT+TGG + chr3.1:92911247-92911266 MS.gene68129:CDS 45.0%
ACACCTGGTCGCGTATACAA+GGG + chr3.1:92911787-92911806 MS.gene68129:CDS 50.0%
ACCTTGAAAACCCTTGCCGA+TGG - chr3.1:92911588-92911607 None:intergenic 50.0%
ACGGGAACAACTTCACCGTT+AGG - chr3.1:92911293-92911312 None:intergenic 50.0%
AGGGAAAGAAGATGCCTGGT+AGG + chr3.1:92911806-92911825 MS.gene68129:CDS 50.0%
ATAGCCCCCGCTAAGATTGT+TGG + chr3.1:92911943-92911962 MS.gene68129:CDS 50.0%
ATGAGCTTAGAGGCTGGTGT+TGG + chr3.1:92911227-92911246 MS.gene68129:CDS 50.0%
ATTTGCGGTCTCTAACCCTC+CGG - chr3.1:92911403-92911422 None:intergenic 50.0%
CAACTTGGATCCATTGGTGC+CGG + chr3.1:92911760-92911779 MS.gene68129:CDS 50.0%
CTCTTGGAACCATGGCTCAT+TGG - chr3.1:92911733-92911752 None:intergenic 50.0%
CTTGCCAACAATCTTAGCGG+GGG - chr3.1:92911950-92911969 None:intergenic 50.0%
GTCTGTGACTCTTGGAACCA+TGG - chr3.1:92911741-92911760 None:intergenic 50.0%
GTTGTTCCCGTCACTGTTGT+CGG + chr3.1:92911302-92911321 MS.gene68129:CDS 50.0%
TAACCCTCCGGTAACCAACT+TGG - chr3.1:92911391-92911410 None:intergenic 50.0%
TACACCTGGTCGCGTATACA+AGG + chr3.1:92911786-92911805 MS.gene68129:CDS 50.0%
TTGAAAACCCTTGCCGATGG+TGG - chr3.1:92911585-92911604 None:intergenic 50.0%
TTTCCCTTGTATACGCGACC+AGG - chr3.1:92911793-92911812 None:intergenic 50.0%
! ACCATCGGCAAGGGTTTTCA+AGG + chr3.1:92911584-92911603 MS.gene68129:CDS 50.0%
AAGATGCCTGGTAGGATGGG+TGG + chr3.1:92911814-92911833 MS.gene68129:CDS 55.0%
AGGTGTAGTTCCGGCACCAA+TGG - chr3.1:92911773-92911792 None:intergenic 55.0%
ATCAAAACCGACGCCACCGA+CGG + chr3.1:92911353-92911372 MS.gene68129:CDS 55.0%
CCTCCGGTAACCAACTTGGA+CGG - chr3.1:92911387-92911406 None:intergenic 55.0%
GATGTCTCTGGCACCACCAT+CGG + chr3.1:92911569-92911588 MS.gene68129:CDS 55.0%
GGTTACAACGCCGTCCAAGT+TGG + chr3.1:92911374-92911393 MS.gene68129:CDS 55.0%
GTAGGATGGGTGGAACCAAC+AGG + chr3.1:92911824-92911843 MS.gene68129:CDS 55.0%
TACGCGACCAGGTGTAGTTC+CGG - chr3.1:92911782-92911801 None:intergenic 55.0%
TTGGACGGCGTTGTAACCGT+CGG - chr3.1:92911372-92911391 None:intergenic 55.0%
TTGGTTCCACCCATCCTACC+AGG - chr3.1:92911823-92911842 None:intergenic 55.0%
!! ACGCCGTCCAAGTTGGTTAC+CGG + chr3.1:92911381-92911400 MS.gene68129:CDS 55.0%
!! AGAGGCTGGTGTTGGTGTGA+TGG + chr3.1:92911235-92911254 MS.gene68129:CDS 55.0%
!! ATTGGTGCCGGAACTACACC+TGG + chr3.1:92911772-92911791 MS.gene68129:CDS 55.0%
!! CGTCCAAGTTGGTTACCGGA+GGG + chr3.1:92911385-92911404 MS.gene68129:CDS 55.0%
!! GAGGCTGGTGTTGGTGTGAT+GGG + chr3.1:92911236-92911255 MS.gene68129:CDS 55.0%
GGTGCTCTTCCTGGTAAGCC+TGG + chr3.1:92911907-92911926 MS.gene68129:CDS 60.0%
GTTGTAACCGTCGGTGGCGT+CGG - chr3.1:92911363-92911382 None:intergenic 60.0%
TCTGGCACCACCATCGGCAA+GGG + chr3.1:92911575-92911594 MS.gene68129:CDS 60.0%
!! CCGTCCAAGTTGGTTACCGG+AGG + chr3.1:92911384-92911403 MS.gene68129:CDS 60.0%
CTCTGGCACCACCATCGGCA+AGG + chr3.1:92911574-92911593 MS.gene68129:CDS 65.0%
GACGGCGTTGTAACCGTCGG+TGG - chr3.1:92911369-92911388 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr3.1 gene 92911215 92911987 92911215 ID=MS.gene68129
chr3.1 mRNA 92911215 92911987 92911215 ID=MS.gene68129.t1;Parent=MS.gene68129
chr3.1 exon 92911215 92911605 92911215 ID=MS.gene68129.t1.exon1;Parent=MS.gene68129.t1
chr3.1 CDS 92911215 92911605 92911215 ID=cds.MS.gene68129.t1;Parent=MS.gene68129.t1
chr3.1 exon 92911698 92911987 92911698 ID=MS.gene68129.t1.exon2;Parent=MS.gene68129.t1
chr3.1 CDS 92911698 92911987 92911698 ID=cds.MS.gene68129.t1;Parent=MS.gene68129.t1
Gene Sequence

>MS.gene68129

ATGGTGGTGAAAATGAGCTTAGAGGCTGGTGTTGGTGTGATGGGTACCAAATTGGGAATGATGAGCTTTTTCCAACCTAACGGTGAAGTTGTTCCCGTCACTGTTGTCGGTTTCAAAGAAGGCAACATCGTCACTCAAATCAAAACCGACGCCACCGACGGTTACAACGCCGTCCAAGTTGGTTACCGGAGGGTTAGAGACCGCAAATTGACCAAACCTGAAATGGGTCATCTTGAAAAGGTTGGAGCCATTCCTATGCGACATCTTCAGGAGTTTCGTCTTCAATCCGTTGATGGTTTTGAACCTAATCAGCGCCTTGTATTTGATGAGCTTTTCAATGAAGGTGATCTTGTTGATGTCTCTGGCACCACCATCGGCAAGGGTTTTCAAGGTCTGTAACTCTCTCCCTCTTTGCATTCCCTTCTAGTTGTTGAGTTGATAATAATATCTTTCACTAATGGATGAACTTACTTACTTTGACAGGTGGTATCAAGCGTCATAACTTCAAAAGAGGACCAATGAGCCATGGTTCCAAGAGTCACAGACAACTTGGATCCATTGGTGCCGGAACTACACCTGGTCGCGTATACAAGGGAAAGAAGATGCCTGGTAGGATGGGTGGAACCAACAGGAAGACCAGAAAGCTTAAGATTGTTAAAATTGATAAAGATCTTAATGTTATCATGATCAAAGGTGCTCTTCCTGGTAAGCCTGGTAATCTTCTACGGATAGCCCCCGCTAAGATTGTTGGCAAGAACATTCCAAAGAATTAA

Protein sequence

>MS.gene68129.t1

MVVKMSLEAGVGVMGTKLGMMSFFQPNGEVVPVTVVGFKEGNIVTQIKTDATDGYNAVQVGYRRVRDRKLTKPEMGHLEKVGAIPMRHLQEFRLQSVDGFEPNQRLVFDELFNEGDLVDVSGTTIGKGFQGGIKRHNFKRGPMSHGSKSHRQLGSIGAGTTPGRVYKGKKMPGRMGGTNRKTRKLKIVKIDKDLNVIMIKGALPGKPGNLLRIAPAKIVGKNIPKN