AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene23141


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene23141.t1 MTR_7g111880 96.000 250 9 1 1 249 1 250 3.30e-172 474
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene23141.t1 AT4G15510 64.659 249 76 5 10 247 40 287 4.09e-105 306
MS.gene23141.t1 AT4G15510 73.039 204 52 2 46 247 3 205 2.02e-103 298
MS.gene23141.t1 AT4G15510 73.039 204 52 2 46 247 3 205 2.02e-103 298
MS.gene23141.t1 AT4G15510 73.039 204 52 2 46 247 3 205 2.02e-103 298
MS.gene23141.t1 AT4G15510 69.595 148 40 3 35 178 72 218 1.67e-62 196

Find 0 sgRNAs with CRISPR-Local

Find 72 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATTCTTGATGAAAATATAT+CGG + 51771:8265-8284 MS.gene23141:CDS 15.0%
!!! ATATTTTCATCAAGAATATA+AGG - 51771:8263-8282 None:intergenic 15.0%
!! AAATAAAATAATTGTGACAG+AGG - 51771:7991-8010 None:intergenic 20.0%
!! TACATTTATCAGAAAAATTG+TGG + 51771:8492-8511 MS.gene23141:intron 20.0%
! GAATATGTTGATACATTTGA+TGG + 51771:8167-8186 MS.gene23141:CDS 25.0%
AAAACTTACGGTAATTTGAG+GGG + 51771:8540-8559 MS.gene23141:intron 30.0%
AGAAAATGTGTTTCAAGAAG+AGG + 51771:8809-8828 MS.gene23141:CDS 30.0%
CAAAACTTACGGTAATTTGA+GGG + 51771:8539-8558 MS.gene23141:intron 30.0%
CATTCAACTATCCATCAAAT+TGG + 51771:8195-8214 MS.gene23141:CDS 30.0%
CTTACTTGAATCCAATTTGA+TGG - 51771:8209-8228 None:intergenic 30.0%
TCAAATTGGATTCAAGTAAG+AGG + 51771:8209-8228 MS.gene23141:CDS 30.0%
! CAAATTGTACTATCAAGTTG+AGG + 51771:8463-8482 MS.gene23141:CDS 30.0%
! TCTTCTTGAAACACATTTTC+TGG - 51771:8810-8829 None:intergenic 30.0%
!! AATGACATTGCTTTTCTTCT+AGG - 51771:8080-8099 None:intergenic 30.0%
ATGGATGGAATTTACCGAAT+TGG - 51771:7954-7973 None:intergenic 35.0%
ATTCAAGACTGTTCAAGACT+TGG + 51771:8316-8335 MS.gene23141:CDS 35.0%
CTCTGTTCTTGCAAAACTTA+CGG + 51771:8528-8547 MS.gene23141:intron 35.0%
GCAAAACTTACGGTAATTTG+AGG + 51771:8538-8557 MS.gene23141:intron 35.0%
GTAATCTCAACTCATACAGT+TGG - 51771:8782-8801 None:intergenic 35.0%
GTCTTGAATTTCGAAGAAGA+AGG - 51771:8305-8324 None:intergenic 35.0%
TGGATGGAATTTACCGAATT+GGG - 51771:7953-7972 None:intergenic 35.0%
TTACTGAGTTTATGTCCACT+AGG + 51771:8381-8400 MS.gene23141:CDS 35.0%
TTCAAGACTGTTCAAGACTT+GGG + 51771:8317-8336 MS.gene23141:CDS 35.0%
! GGGTGTCTTTTATATGGTAA+TGG + 51771:8560-8579 MS.gene23141:intron 35.0%
!!! TGGTGCTGATATTTTCTTCA+GGG + 51771:8235-8254 MS.gene23141:CDS 35.0%
!!! TTTGAGGGGTGTCTTTTATA+TGG + 51771:8554-8573 MS.gene23141:intron 35.0%
ACATATTCACGAAACACACG+AGG - 51771:8155-8174 None:intergenic 40.0%
ACCCGTTGTTGTGATTGTAT+TGG - 51771:7867-7886 None:intergenic 40.0%
ACTTACATTCTCTCCGACAT+TGG + 51771:8110-8129 MS.gene23141:CDS 40.0%
AGAGGAGAAAGCTCATGAAA+TGG - 51771:7973-7992 None:intergenic 40.0%
ATGGAAGTGAAATGGTGACA+TGG - 51771:7903-7922 None:intergenic 40.0%
GAATTTACCGAATTGGGTGT+GGG - 51771:7947-7966 None:intergenic 40.0%
GAGAAAGCTCATGAAATGGA+TGG - 51771:7969-7988 None:intergenic 40.0%
GCCAATACAATCACAACAAC+GGG + 51771:7863-7882 MS.gene23141:CDS 40.0%
GGCCAATACAATCACAACAA+CGG + 51771:7862-7881 MS.gene23141:CDS 40.0%
TAGCGCTAGACTACAATGTA+TGG - 51771:8588-8607 None:intergenic 40.0%
TGCTAATGCTGTACCAATGT+CGG - 51771:8126-8145 None:intergenic 40.0%
TGCTGTTATGCCACAAGATA+GGG + 51771:8701-8720 MS.gene23141:CDS 40.0%
TGGAAGTGAAATGGTGACAT+GGG - 51771:7902-7921 None:intergenic 40.0%
TTTCAACTCCTAGAACAGAC+AGG - 51771:8755-8774 None:intergenic 40.0%
! TTGCTGTTATGCCACAAGAT+AGG + 51771:8700-8719 MS.gene23141:CDS 40.0%
!! TGTGTGTTGTTTGTGTTGCT+AGG + 51771:8644-8663 MS.gene23141:intron 40.0%
!!! CTGGTGCTGATATTTTCTTC+AGG + 51771:8234-8253 MS.gene23141:CDS 40.0%
ACGTTTAACACCAAGCCTAG+TGG - 51771:8399-8418 None:intergenic 45.0%
AGTGAAATGGTGACATGGGT+CGG - 51771:7898-7917 None:intergenic 45.0%
ATCATCAGCAACTCTCTCAG+AGG - 51771:8441-8460 None:intergenic 45.0%
CATTTCTCCCACACCCAATT+CGG + 51771:7937-7956 MS.gene23141:CDS 45.0%
GAGTGAACTTCGTCAAGTCA+TGG + 51771:8833-8852 MS.gene23141:CDS 45.0%
GAGTTTATGTCCACTAGGCT+TGG + 51771:8386-8405 MS.gene23141:CDS 45.0%
GGAATTTACCGAATTGGGTG+TGG - 51771:7948-7967 None:intergenic 45.0%
GGCTTCGTTTCGAGCAAATA+AGG + 51771:8854-8873 MS.gene23141:CDS 45.0%
TCTGAGAGAGTTGCTGATGA+TGG + 51771:8440-8459 MS.gene23141:CDS 45.0%
TGTTATGCCACAAGATAGGG+TGG + 51771:8704-8723 MS.gene23141:CDS 45.0%
! TGGATTCAAGTAAGAGGTGC+TGG + 51771:8215-8234 MS.gene23141:CDS 45.0%
!! ACTTTCTTCCCAGCTTGTTG+AGG - 51771:8347-8366 None:intergenic 45.0%
AAACGCACCACCCTATCTTG+TGG - 51771:8714-8733 None:intergenic 50.0%
ACAAGATAGGGTGGTGCGTT+TGG + 51771:8713-8732 MS.gene23141:CDS 50.0%
ATAGGGTGGTGCGTTTGGAA+TGG + 51771:8718-8737 MS.gene23141:CDS 50.0%
ATCAGCAACTCTCTCAGAGG+TGG - 51771:8438-8457 None:intergenic 50.0%
CGAATTGGGTGTGGGAGAAA+TGG - 51771:7939-7958 None:intergenic 50.0%
CGAGGGGAATGGAAGTGAAA+TGG - 51771:7911-7930 None:intergenic 50.0%
GTGAAATGGTGACATGGGTC+GGG - 51771:7897-7916 None:intergenic 50.0%
! TAGGGTGGTGCGTTTGGAAT+GGG + 51771:8719-8738 MS.gene23141:CDS 50.0%
! TGCGTTTGGAATGGGATAGG+AGG + 51771:8727-8746 MS.gene23141:CDS 50.0%
! TGGTGCGTTTGGAATGGGAT+AGG + 51771:8724-8743 MS.gene23141:CDS 50.0%
!! AGGAGGTACCTGTCTGTTCT+AGG + 51771:8744-8763 MS.gene23141:CDS 50.0%
AAATGGAGCAGCGAGGGGAA+TGG - 51771:7922-7941 None:intergenic 55.0%
TGGGAGAAATGGAGCAGCGA+GGG - 51771:7928-7947 None:intergenic 55.0%
TGGGTTCGCCTCAACAAGCT+GGG + 51771:8336-8355 MS.gene23141:CDS 55.0%
TTGGGTTCGCCTCAACAAGC+TGG + 51771:8335-8354 MS.gene23141:CDS 55.0%
GGGAGAAATGGAGCAGCGAG+GGG - 51771:7927-7946 None:intergenic 60.0%
GTGGGAGAAATGGAGCAGCG+AGG - 51771:7929-7948 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
51771 gene 7860 8887 7860 ID=MS.gene23141
51771 mRNA 7860 8887 7860 ID=MS.gene23141.t1;Parent=MS.gene23141
51771 exon 7860 7958 7860 ID=MS.gene23141.t1.exon1;Parent=MS.gene23141.t1
51771 CDS 7860 7958 7860 ID=cds.MS.gene23141.t1;Parent=MS.gene23141.t1
51771 exon 8056 8484 8056 ID=MS.gene23141.t1.exon2;Parent=MS.gene23141.t1
51771 CDS 8056 8484 8056 ID=cds.MS.gene23141.t1;Parent=MS.gene23141.t1
51771 exon 8666 8887 8666 ID=MS.gene23141.t1.exon3;Parent=MS.gene23141.t1
51771 CDS 8666 8887 8666 ID=cds.MS.gene23141.t1;Parent=MS.gene23141.t1
Gene Sequence

>MS.gene23141

ATGGCCAATACAATCACAACAACGGGTTTTCTAGCCCGACCCATGTCACCATTTCACTTCCATTCCCCTCGCTGCTCCATTTCTCCCACACCCAATTCGGTAAATTCCATCCATTTCATGAGCTTTCTCCTCTGTCACAATTATTTTATTTTATTTACTAAATCTTTGATTTTTGTCTCTGCTAATATATAATCAGATTAAAGCAGCTTTTGCAGTTCCTAGAAGAAAAGCAATGTCATTAATCTTATCAACTTACATTCTCTCCGACATTGGTACAGCATTAGCACAACAACCTCGTGTGTTTCGTGAATATGTTGATACATTTGATGGTTATTCATTCAACTATCCATCAAATTGGATTCAAGTAAGAGGTGCTGGTGCTGATATTTTCTTCAGGGATCCTTATATTCTTGATGAAAATATATCGGTCGAAGTTTCTTCGCCTTCTTCTTCGAAATTCAAGACTGTTCAAGACTTGGGTTCGCCTCAACAAGCTGGGAAGAAAGTTCTTGATCAGTATCTTACTGAGTTTATGTCCACTAGGCTTGGTGTTAAACGTGAATCTAATATTCTTTCCACCTCTGAGAGAGTTGCTGATGATGGCAAATTGTACTATCAAGTTGAGGTTCGTTTACATTTATCAGAAAAATTGTGGTTAATCACGTTAACTCTGTTCTTGCAAAACTTACGGTAATTTGAGGGGTGTCTTTTATATGGTAATGGAACCATACATTGTAGTCTAGCGCTACTTGTTGCAAAATGACATTAAATGATTGATTGATTGTGTGTGTTGTTTGTGTTGCTAGGCGAACATAAAGTCATATGCTAGTAACAACGAGCTTGCTGTTATGCCACAAGATAGGGTGGTGCGTTTGGAATGGGATAGGAGGTACCTGTCTGTTCTAGGAGTTGAAAACAACCAACTGTATGAGTTGAGATTACAAGTGCCAGAAAATGTGTTTCAAGAAGAGGAGAGTGAACTTCGTCAAGTCATGGCTTCGTTTCGAGCAAATAAGGTTGTTCGTTAG

Protein sequence

>MS.gene23141.t1

MANTITTTGFLARPMSPFHFHSPRCSISPTPNSIKAAFAVPRRKAMSLILSTYILSDIGTALAQQPRVFREYVDTFDGYSFNYPSNWIQVRGAGADIFFRDPYILDENISVEVSSPSSSKFKTVQDLGSPQQAGKKVLDQYLTEFMSTRLGVKRESNILSTSERVADDGKLYYQVEANIKSYASNNELAVMPQDRVVRLEWDRRYLSVLGVENNQLYELRLQVPENVFQEEESELRQVMASFRANKVVR