AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene061683


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene061683.t1 MTR_1g034320 96.761 247 8 0 1 247 1 247 5.98e-178 498
MS.gene061683.t1 MTR_1g034330 82.072 251 41 1 1 247 1 251 1.50e-151 431
MS.gene061683.t1 MTR_4g068550 63.968 247 85 3 1 247 1 243 5.73e-109 322
MS.gene061683.t1 MTR_3g111530 50.847 236 110 4 13 247 89 319 8.99e-71 226
MS.gene061683.t1 MTR_1g021652 48.333 240 118 4 8 247 52 285 9.23e-70 223
MS.gene061683.t1 MTR_2g087890 39.149 235 137 4 14 246 22 252 7.20e-44 154
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene061683.t1 AT5G42650 46.610 236 121 3 12 247 49 279 1.40e-68 220
MS.gene061683.t1 AT4G15440 36.184 152 94 2 94 245 4 152 4.73e-19 85.5

Find 50 sgRNAs with CRISPR-Local

Find 64 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAGATTGAAGATGGGCTTTC+TGG 0.174432 1.3:+19054315 MS.gene061683:CDS
CTTGCTCCACTTGCAACTTT+AGG 0.254713 1.3:+19054486 MS.gene061683:CDS
TCAAGATAATTGAAAATCTT+AGG 0.276501 1.3:-19054513 None:intergenic
TGCTATGACTCTTGGGTTTG+AGG 0.356639 1.3:-19054052 None:intergenic
GTCCTAAAATGTTTGATACT+TGG 0.383207 1.3:+19054451 MS.gene061683:CDS
TCTAAAAGTGCTATGACTCT+TGG 0.437203 1.3:-19054060 None:intergenic
CTAAAAGTGCTATGACTCTT+GGG 0.446715 1.3:-19054059 None:intergenic
AGAAGAATTTGTCTCTTCCC+TGG 0.447129 1.3:-19053963 None:intergenic
CCTTTCCTGCTTGTTTGACA+AGG 0.452440 1.3:+19054559 MS.gene061683:CDS
AAAGGGAGGCCATAGCTGCC+TGG 0.458877 1.3:-19053901 None:intergenic
CCTTTGATAAGTGCATGGTT+TGG 0.464225 1.3:-19054213 None:intergenic
AAACAAATCCCAGGCAGCTA+TGG 0.478988 1.3:+19053892 MS.gene061683:CDS
TCTTGGGTTTGAGGAAATGA+AGG 0.480085 1.3:-19054043 None:intergenic
GGTTTGGTTCAGTTGTGTCT+AGG 0.483967 1.3:-19054197 None:intergenic
CCTTGTCAAACAAGCAGGAA+AGG 0.488909 1.3:-19054559 None:intergenic
TAGTGGGAGCTCTTTGTTGG+TGG 0.491046 1.3:-19053869 None:intergenic
GAAACTATCCTTGGTGATCA+AGG 0.500468 1.3:+19054429 MS.gene061683:CDS
AACCCTTCTGAAACTATCCT+TGG 0.510155 1.3:+19054420 MS.gene061683:CDS
TAGTCGTGTCGGTCGAAGAT+CGG 0.514893 1.3:-19053931 None:intergenic
TTCAGAACCAACATGCCTCC+AGG 0.517385 1.3:+19054018 MS.gene061683:CDS
CGAAGATCGGTCCGATGAAA+GGG 0.519863 1.3:-19053918 None:intergenic
GATGGGCTTTCTGGTAAAAG+TGG 0.524979 1.3:+19054324 MS.gene061683:CDS
TCGAAGATCGGTCCGATGAA+AGG 0.525790 1.3:-19053919 None:intergenic
AGCTATGGCCTCCCTTTCAT+CGG 0.539465 1.3:+19053907 MS.gene061683:CDS
AAGGGAGGCCATAGCTGCCT+GGG 0.542022 1.3:-19053900 None:intergenic
CCAAACCATGCACTTATCAA+AGG 0.547827 1.3:+19054213 MS.gene061683:CDS
CTTGTCAAACAAGCAGGAAA+GGG 0.552808 1.3:-19054558 None:intergenic
AAAGTATAGTCTTAAAGAGA+GGG 0.558116 1.3:-19054278 None:intergenic
GAAAGTATAGTCTTAAAGAG+AGG 0.560352 1.3:-19054279 None:intergenic
AGAAACCTTTGATAAGTGCA+TGG 0.573003 1.3:-19054218 None:intergenic
CACGACTACTTCTACAACCA+GGG 0.577411 1.3:+19053946 MS.gene061683:CDS
TCTCACCTTGTCAAACAAGC+AGG 0.589767 1.3:-19054564 None:intergenic
ACACGACTACTTCTACAACC+AGG 0.590429 1.3:+19053945 MS.gene061683:CDS
GAAGGGACCTGGAGGCATGT+TGG 0.593712 1.3:-19054025 None:intergenic
TCACCAAGGATAGTTTCAGA+AGG 0.597461 1.3:-19054423 None:intergenic
CACCAAGGATAGTTTCAGAA+GGG 0.600780 1.3:-19054422 None:intergenic
AGATAATTGAAAATCTTAGG+AGG 0.601465 1.3:-19054510 None:intergenic
TTTGAGGAAATGAAGGGACC+TGG 0.609736 1.3:-19054036 None:intergenic
AGCACATACACGGTAACCAC+CGG 0.615577 1.3:-19054172 None:intergenic
CTCCCACTAAAACAAATCCC+AGG 0.620738 1.3:+19053883 MS.gene061683:CDS
GGAGGTCCTAAAGTTGCAAG+TGG 0.621056 1.3:-19054492 None:intergenic
CTTGGGTTTGAGGAAATGAA+GGG 0.627221 1.3:-19054042 None:intergenic
ATGCCGTCGACAAAATTCAC+CGG 0.643799 1.3:+19054153 MS.gene061683:CDS
AGATCGGTCCGATGAAAGGG+AGG 0.647150 1.3:-19053915 None:intergenic
GGTTGTAGAAGTAGTCGTGT+CGG 0.648799 1.3:-19053942 None:intergenic
ACGGCATGTAAGTGCCGTCG+AGG 0.653207 1.3:-19054137 None:intergenic
GAAGAGGATCGGGAAAGATG+CGG 0.653874 1.3:-19054085 None:intergenic
GAGGAAATGAAGGGACCTGG+AGG 0.681898 1.3:-19054033 None:intergenic
CCGTCGACAAAATTCACCGG+TGG 0.696972 1.3:+19054156 MS.gene061683:CDS
TGTCTAGGTAAGCACATACA+CGG 0.697884 1.3:-19054182 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TCAAGATAATTGAAAATCTT+AGG - chr1.3:19054516-19054535 None:intergenic 20.0%
!!! AGATTGTTGAATTGTATTTT+TGG - chr1.3:19053999-19054018 None:intergenic 20.0%
! AGATAATTGAAAATCTTAGG+AGG - chr1.3:19054513-19054532 None:intergenic 25.0%
!! AAAGTATAGTCTTAAAGAGA+GGG - chr1.3:19054281-19054300 None:intergenic 25.0%
!! AACCAAGTATCAAACATTTT+AGG - chr1.3:19054456-19054475 None:intergenic 25.0%
!!! TTTTAACTCTATTATGAGTG+TGG + chr1.3:19054356-19054375 MS.gene061683:CDS 25.0%
GTCCTAAAATGTTTGATACT+TGG + chr1.3:19054451-19054470 MS.gene061683:CDS 30.0%
! CTTTTAGTGAGATTGAAGAT+GGG + chr1.3:19054307-19054326 MS.gene061683:CDS 30.0%
! GAAAGTATAGTCTTAAAGAG+AGG - chr1.3:19054282-19054301 None:intergenic 30.0%
! TGAAGATATTTTGCTACGTA+CGG + chr1.3:19054533-19054552 MS.gene061683:CDS 30.0%
AGAAACCTTTGATAAGTGCA+TGG - chr1.3:19054221-19054240 None:intergenic 35.0%
! GCTTTTAGTGAGATTGAAGA+TGG + chr1.3:19054306-19054325 MS.gene061683:CDS 35.0%
!! CTAAAAGTGCTATGACTCTT+GGG - chr1.3:19054062-19054081 None:intergenic 35.0%
!! TCTAAAAGTGCTATGACTCT+TGG - chr1.3:19054063-19054082 None:intergenic 35.0%
!! TTTTTGTTGTCGAAGAGGAT+CGG - chr1.3:19054099-19054118 None:intergenic 35.0%
AGAAGAATTTGTCTCTTCCC+TGG - chr1.3:19053966-19053985 None:intergenic 40.0%
CACCAAGGATAGTTTCAGAA+GGG - chr1.3:19054425-19054444 None:intergenic 40.0%
CCAAACCATGCACTTATCAA+AGG + chr1.3:19054213-19054232 MS.gene061683:CDS 40.0%
CCTTTGATAAGTGCATGGTT+TGG - chr1.3:19054216-19054235 None:intergenic 40.0%
CTTGGGTTTGAGGAAATGAA+GGG - chr1.3:19054045-19054064 None:intergenic 40.0%
CTTGTCAAACAAGCAGGAAA+GGG - chr1.3:19054561-19054580 None:intergenic 40.0%
TCACCAAGGATAGTTTCAGA+AGG - chr1.3:19054426-19054445 None:intergenic 40.0%
TCTTGGGTTTGAGGAAATGA+AGG - chr1.3:19054046-19054065 None:intergenic 40.0%
TGTCTAGGTAAGCACATACA+CGG - chr1.3:19054185-19054204 None:intergenic 40.0%
! GAAACTATCCTTGGTGATCA+AGG + chr1.3:19054429-19054448 MS.gene061683:CDS 40.0%
! TTTTAGTGGGAGCTCTTTGT+TGG - chr1.3:19053875-19053894 None:intergenic 40.0%
!! AACCCTTCTGAAACTATCCT+TGG + chr1.3:19054420-19054439 MS.gene061683:CDS 40.0%
!! TTTTGTTGTCGAAGAGGATC+GGG - chr1.3:19054098-19054117 None:intergenic 40.0%
!!! CGACTTTTTTGTTGTCGAAG+AGG - chr1.3:19054104-19054123 None:intergenic 40.0%
!!! TGCCTGGGATTTGTTTTAGT+GGG - chr1.3:19053888-19053907 None:intergenic 40.0%
!!! TTTTAGGACCTTGATCACCA+AGG - chr1.3:19054440-19054459 None:intergenic 40.0%
AAACAAATCCCAGGCAGCTA+TGG + chr1.3:19053892-19053911 MS.gene061683:CDS 45.0%
ACACGACTACTTCTACAACC+AGG + chr1.3:19053945-19053964 MS.gene061683:CDS 45.0%
ATGCCGTCGACAAAATTCAC+CGG + chr1.3:19054153-19054172 MS.gene061683:CDS 45.0%
CACGACTACTTCTACAACCA+GGG + chr1.3:19053946-19053965 MS.gene061683:CDS 45.0%
CCTTGTCAAACAAGCAGGAA+AGG - chr1.3:19054562-19054581 None:intergenic 45.0%
CCTTTCCTGCTTGTTTGACA+AGG + chr1.3:19054559-19054578 MS.gene061683:CDS 45.0%
CTCCCACTAAAACAAATCCC+AGG + chr1.3:19053883-19053902 MS.gene061683:CDS 45.0%
CTTGCTCCACTTGCAACTTT+AGG + chr1.3:19054486-19054505 MS.gene061683:CDS 45.0%
GAAAAACTCAACGTCCTCGA+CGG + chr1.3:19054123-19054142 MS.gene061683:CDS 45.0%
GGTTGTAGAAGTAGTCGTGT+CGG - chr1.3:19053945-19053964 None:intergenic 45.0%
TGCTATGACTCTTGGGTTTG+AGG - chr1.3:19054055-19054074 None:intergenic 45.0%
TTTGAGGAAATGAAGGGACC+TGG - chr1.3:19054039-19054058 None:intergenic 45.0%
! GAGATTGAAGATGGGCTTTC+TGG + chr1.3:19054315-19054334 MS.gene061683:CDS 45.0%
! GATGGGCTTTCTGGTAAAAG+TGG + chr1.3:19054324-19054343 MS.gene061683:CDS 45.0%
! GGTTTGGTTCAGTTGTGTCT+AGG - chr1.3:19054200-19054219 None:intergenic 45.0%
!!! CTGCCTGGGATTTGTTTTAG+TGG - chr1.3:19053889-19053908 None:intergenic 45.0%
AGCACATACACGGTAACCAC+CGG - chr1.3:19054175-19054194 None:intergenic 50.0%
AGCTATGGCCTCCCTTTCAT+CGG + chr1.3:19053907-19053926 MS.gene061683:CDS 50.0%
CGAAGATCGGTCCGATGAAA+GGG - chr1.3:19053921-19053940 None:intergenic 50.0%
GAAGAGGATCGGGAAAGATG+CGG - chr1.3:19054088-19054107 None:intergenic 50.0%
GGAGGTCCTAAAGTTGCAAG+TGG - chr1.3:19054495-19054514 None:intergenic 50.0%
TAGTGGGAGCTCTTTGTTGG+TGG - chr1.3:19053872-19053891 None:intergenic 50.0%
TCGAAGATCGGTCCGATGAA+AGG - chr1.3:19053922-19053941 None:intergenic 50.0%
TTCAGAACCAACATGCCTCC+AGG + chr1.3:19054018-19054037 MS.gene061683:CDS 50.0%
! CCACCGGTGAATTTTGTCGA+CGG - chr1.3:19054159-19054178 None:intergenic 50.0%
! TAGTCGTGTCGGTCGAAGAT+CGG - chr1.3:19053934-19053953 None:intergenic 50.0%
AGATCGGTCCGATGAAAGGG+AGG - chr1.3:19053918-19053937 None:intergenic 55.0%
CCGTCGACAAAATTCACCGG+TGG + chr1.3:19054156-19054175 MS.gene061683:CDS 55.0%
GAGGAAATGAAGGGACCTGG+AGG - chr1.3:19054036-19054055 None:intergenic 55.0%
AAAGGGAGGCCATAGCTGCC+TGG - chr1.3:19053904-19053923 None:intergenic 60.0%
AAGGGAGGCCATAGCTGCCT+GGG - chr1.3:19053903-19053922 None:intergenic 60.0%
! ACGGCATGTAAGTGCCGTCG+AGG - chr1.3:19054140-19054159 None:intergenic 60.0%
! GAAGGGACCTGGAGGCATGT+TGG - chr1.3:19054028-19054047 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr1.3 gene 19053841 19054584 19053841 ID=MS.gene061683
chr1.3 mRNA 19053841 19054584 19053841 ID=MS.gene061683.t1;Parent=MS.gene061683
chr1.3 exon 19053841 19054584 19053841 ID=MS.gene061683.t1.exon1;Parent=MS.gene061683.t1
chr1.3 CDS 19053841 19054584 19053841 ID=cds.MS.gene061683.t1;Parent=MS.gene061683.t1
Gene Sequence

>MS.gene061683

ATGGCATCTTCAAAACAAGAACAATCTTCCACCAACAAAGAGCTCCCACTAAAACAAATCCCAGGCAGCTATGGCCTCCCTTTCATCGGACCGATCTTCGACCGACACGACTACTTCTACAACCAGGGAAGAGACAAATTCTTCTCAACAAGAATCCAAAAATACAATTCAACAATCTTCAGAACCAACATGCCTCCAGGTCCCTTCATTTCCTCAAACCCAAGAGTCATAGCACTTTTAGACGCCGCATCTTTCCCGATCCTCTTCGACAACAAAAAAGTCGAAAAACTCAACGTCCTCGACGGCACTTACATGCCGTCGACAAAATTCACCGGTGGTTACCGTGTATGTGCTTACCTAGACACAACTGAACCAAACCATGCACTTATCAAAGGTTTCTATCTTAATACTCTTTTATTAAGAAAAGACTCTTTTATCCCTCTCTTTAAGACTATACTTTCTGATGCTTTTAGTGAGATTGAAGATGGGCTTTCTGGTAAAAGTGGTAAAGCTGATTTTAACTCTATTATGAGTGTGGCTTCGTTTAACTTCATGTTTAAGCTTTTTTGTGATAATAAGAACCCTTCTGAAACTATCCTTGGTGATCAAGGTCCTAAAATGTTTGATACTTGGTTACTCTTTCAACTTGCTCCACTTGCAACTTTAGGACCTCCTAAGATTTTCAATTATCTTGAAGATATTTTGCTACGTACGGTGCCCTTTCCTGCTTGTTTGACAAGGTGA

Protein sequence

>MS.gene061683.t1

MASSKQEQSSTNKELPLKQIPGSYGLPFIGPIFDRHDYFYNQGRDKFFSTRIQKYNSTIFRTNMPPGPFISSNPRVIALLDAASFPILFDNKKVEKLNVLDGTYMPSTKFTGGYRVCAYLDTTEPNHALIKGFYLNTLLLRKDSFIPLFKTILSDAFSEIEDGLSGKSGKADFNSIMSVASFNFMFKLFCDNKNPSETILGDQGPKMFDTWLLFQLAPLATLGPPKIFNYLEDILLRTVPFPACLTR