Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene21037.t1 | RHN80872.1 | 98.3 | 173 | 2 | 1 | 1 | 172 | 32 | 204 | 1.40E-86 | 328.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene21037.t1 | B7FM98 | 98.3 | 173 | 2 | 1 | 1 | 172 | 1 | 173 | 9.8e-87 | 328.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049346 | MS.gene21037 | 0.8364 | 9.57E-57 | -1.69E-46 |
| MS.gene049351 | MS.gene21037 | 0.808837 | 2.55E-50 | -1.69E-46 |
| MS.gene049356 | MS.gene21037 | 0.867672 | 1.15E-65 | -1.69E-46 |
| MS.gene049369 | MS.gene21037 | 0.847527 | 1.09E-59 | -1.69E-46 |
| MS.gene049661 | MS.gene21037 | 0.812589 | 3.93E-51 | -1.69E-46 |
| MS.gene049662 | MS.gene21037 | 0.80858 | 2.89E-50 | -1.69E-46 |
| MS.gene049758 | MS.gene21037 | 0.82227 | 2.59E-53 | -1.69E-46 |
| MS.gene049907 | MS.gene21037 | 0.813213 | 2.87E-51 | -1.69E-46 |
| MS.gene050122 | MS.gene21037 | 0.812947 | 3.28E-51 | -1.69E-46 |
| MS.gene050301 | MS.gene21037 | 0.852664 | 3.98E-61 | -1.69E-46 |
| MS.gene050397 | MS.gene21037 | 0.82326 | 1.52E-53 | -1.69E-46 |
| MS.gene050432 | MS.gene21037 | 0.859282 | 4.61E-63 | -1.69E-46 |
| MS.gene050520 | MS.gene21037 | 0.829036 | 6.44E-55 | -1.69E-46 |
| MS.gene050670 | MS.gene21037 | 0.856847 | 2.44E-62 | -1.69E-46 |
| MS.gene05101 | MS.gene21037 | 0.80065 | 1.30E-48 | -1.69E-46 |
| MS.gene05105 | MS.gene21037 | 0.875258 | 3.55E-68 | -1.69E-46 |
| MS.gene051255 | MS.gene21037 | 0.850626 | 1.50E-60 | -1.69E-46 |
| MS.gene051360 | MS.gene21037 | 0.864316 | 1.33E-64 | -1.69E-46 |
| MS.gene051387 | MS.gene21037 | 0.809042 | 2.30E-50 | -1.69E-46 |
| MS.gene051525 | MS.gene21037 | 0.842211 | 2.97E-58 | -1.69E-46 |
| MS.gene051582 | MS.gene21037 | 0.813297 | 2.75E-51 | -1.69E-46 |
| MS.gene051692 | MS.gene21037 | 0.827684 | 1.37E-54 | -1.69E-46 |
| MS.gene052011 | MS.gene21037 | -0.809294 | 2.03E-50 | -1.69E-46 |
| MS.gene052077 | MS.gene21037 | 0.804224 | 2.39E-49 | -1.69E-46 |
| MS.gene052078 | MS.gene21037 | 0.803678 | 3.11E-49 | -1.69E-46 |
| MS.gene052136 | MS.gene21037 | 0.805734 | 1.16E-49 | -1.69E-46 |
| MS.gene052188 | MS.gene21037 | 0.853883 | 1.78E-61 | -1.69E-46 |
| MS.gene052253 | MS.gene21037 | 0.853522 | 2.26E-61 | -1.69E-46 |
| MS.gene052310 | MS.gene21037 | 0.839923 | 1.18E-57 | -1.69E-46 |
| MS.gene052322 | MS.gene21037 | 0.827732 | 1.33E-54 | -1.69E-46 |
| MS.gene052340 | MS.gene21037 | 0.872105 | 4.11E-67 | -1.69E-46 |
| MS.gene052414 | MS.gene21037 | 0.832538 | 8.93E-56 | -1.69E-46 |
| MS.gene052421 | MS.gene21037 | 0.828556 | 8.42E-55 | -1.69E-46 |
| MS.gene052481 | MS.gene21037 | 0.839276 | 1.75E-57 | -1.69E-46 |
| MS.gene052484 | MS.gene21037 | 0.818005 | 2.46E-52 | -1.69E-46 |
| MS.gene052485 | MS.gene21037 | 0.877999 | 4.00E-69 | -1.69E-46 |
| MS.gene052508 | MS.gene21037 | 0.811595 | 6.47E-51 | -1.69E-46 |
| MS.gene052982 | MS.gene21037 | 0.827145 | 1.84E-54 | -1.69E-46 |
| MS.gene052999 | MS.gene21037 | 0.816133 | 6.47E-52 | -1.69E-46 |
| MS.gene053103 | MS.gene21037 | 0.814943 | 1.19E-51 | -1.69E-46 |
| MS.gene053104 | MS.gene21037 | 0.802734 | 4.88E-49 | -1.69E-46 |
| MS.gene053169 | MS.gene21037 | 0.811247 | 7.70E-51 | -1.69E-46 |
| MS.gene053207 | MS.gene21037 | 0.804454 | 2.14E-49 | -1.69E-46 |
| MS.gene053209 | MS.gene21037 | 0.883894 | 3.05E-71 | -1.69E-46 |
| MS.gene053449 | MS.gene21037 | 0.831161 | 1.95E-55 | -1.69E-46 |
| MS.gene053557 | MS.gene21037 | 0.81729 | 3.56E-52 | -1.69E-46 |
| MS.gene053813 | MS.gene21037 | 0.83578 | 1.37E-56 | -1.69E-46 |
| MS.gene053836 | MS.gene21037 | 0.811556 | 6.60E-51 | -1.69E-46 |
| MS.gene053928 | MS.gene21037 | 0.864586 | 1.09E-64 | -1.69E-46 |
| MS.gene054010 | MS.gene21037 | 0.820138 | 8.03E-53 | -1.69E-46 |
| MS.gene054011 | MS.gene21037 | 0.814489 | 1.50E-51 | -1.69E-46 |
| MS.gene054012 | MS.gene21037 | 0.822064 | 2.89E-53 | -1.69E-46 |
| MS.gene054013 | MS.gene21037 | 0.820912 | 5.34E-53 | -1.69E-46 |
| MS.gene054123 | MS.gene21037 | 0.801702 | 7.95E-49 | -1.69E-46 |
| MS.gene054349 | MS.gene21037 | 0.805541 | 1.27E-49 | -1.69E-46 |
| MS.gene054556 | MS.gene21037 | 0.837382 | 5.37E-57 | -1.69E-46 |
| MS.gene05505 | MS.gene21037 | 0.816984 | 4.17E-52 | -1.69E-46 |
| MS.gene055116 | MS.gene21037 | 0.891327 | 4.39E-74 | -1.69E-46 |
| MS.gene055277 | MS.gene21037 | 0.838037 | 3.65E-57 | -1.69E-46 |
| MS.gene055422 | MS.gene21037 | 0.801461 | 8.91E-49 | -1.69E-46 |
| MS.gene055428 | MS.gene21037 | 0.814971 | 1.17E-51 | -1.69E-46 |
| MS.gene05553 | MS.gene21037 | 0.818871 | 1.56E-52 | -1.69E-46 |
| MS.gene055604 | MS.gene21037 | 0.831083 | 2.04E-55 | -1.69E-46 |
| MS.gene055633 | MS.gene21037 | 0.811353 | 7.31E-51 | -1.69E-46 |
| MS.gene055666 | MS.gene21037 | 0.814718 | 1.34E-51 | -1.69E-46 |
| MS.gene055722 | MS.gene21037 | 0.827017 | 1.97E-54 | -1.69E-46 |
| MS.gene055741 | MS.gene21037 | 0.81491 | 1.21E-51 | -1.69E-46 |
| MS.gene055744 | MS.gene21037 | 0.818744 | 1.67E-52 | -1.69E-46 |
| MS.gene055802 | MS.gene21037 | 0.802037 | 6.78E-49 | -1.69E-46 |
| MS.gene055909 | MS.gene21037 | 0.820099 | 8.20E-53 | -1.69E-46 |
| MS.gene055911 | MS.gene21037 | 0.825372 | 4.86E-54 | -1.69E-46 |
| MS.gene05592 | MS.gene21037 | 0.80352 | 3.35E-49 | -1.69E-46 |
| MS.gene05602 | MS.gene21037 | 0.810535 | 1.10E-50 | -1.69E-46 |
| MS.gene056191 | MS.gene21037 | 0.86928 | 3.49E-66 | -1.69E-46 |
| MS.gene056407 | MS.gene21037 | 0.856143 | 3.93E-62 | -1.69E-46 |
| MS.gene056410 | MS.gene21037 | 0.856431 | 3.23E-62 | -1.69E-46 |
| MS.gene056422 | MS.gene21037 | 0.805578 | 1.25E-49 | -1.69E-46 |
| MS.gene056511 | MS.gene21037 | 0.833434 | 5.35E-56 | -1.69E-46 |
| MS.gene056659 | MS.gene21037 | 0.811358 | 7.29E-51 | -1.69E-46 |
| MS.gene056723 | MS.gene21037 | 0.80143 | 9.04E-49 | -1.69E-46 |
| MS.gene056796 | MS.gene21037 | 0.821649 | 3.61E-53 | -1.69E-46 |
| MS.gene056902 | MS.gene21037 | 0.834217 | 3.41E-56 | -1.69E-46 |
| MS.gene05702 | MS.gene21037 | 0.826198 | 3.09E-54 | -1.69E-46 |
| MS.gene057124 | MS.gene21037 | 0.843305 | 1.52E-58 | -1.69E-46 |
| MS.gene057330 | MS.gene21037 | 0.812182 | 4.82E-51 | -1.69E-46 |
| MS.gene057331 | MS.gene21037 | 0.812284 | 4.58E-51 | -1.69E-46 |
| MS.gene05733 | MS.gene21037 | 0.815779 | 7.76E-52 | -1.69E-46 |
| MS.gene057396 | MS.gene21037 | 0.826639 | 2.43E-54 | -1.69E-46 |
| MS.gene057448 | MS.gene21037 | 0.840624 | 7.77E-58 | -1.69E-46 |
| MS.gene057449 | MS.gene21037 | 0.82215 | 2.76E-53 | -1.69E-46 |
| MS.gene057450 | MS.gene21037 | 0.839851 | 1.24E-57 | -1.69E-46 |
| MS.gene057469 | MS.gene21037 | 0.806486 | 8.04E-50 | -1.69E-46 |
| MS.gene057734 | MS.gene21037 | 0.801416 | 9.10E-49 | -1.69E-46 |
| MS.gene057749 | MS.gene21037 | 0.852096 | 5.78E-61 | -1.69E-46 |
| MS.gene057752 | MS.gene21037 | 0.800369 | 1.49E-48 | -1.69E-46 |
| MS.gene057823 | MS.gene21037 | 0.859296 | 4.56E-63 | -1.69E-46 |
| MS.gene058002 | MS.gene21037 | 0.848792 | 4.89E-60 | -1.69E-46 |
| MS.gene058006 | MS.gene21037 | 0.805143 | 1.54E-49 | -1.69E-46 |
| MS.gene058007 | MS.gene21037 | 0.825852 | 3.74E-54 | -1.69E-46 |
| MS.gene058082 | MS.gene21037 | 0.807814 | 4.21E-50 | -1.69E-46 |
| MS.gene05811 | MS.gene21037 | 0.847761 | 9.43E-60 | -1.69E-46 |
| MS.gene058293 | MS.gene21037 | 0.851646 | 7.75E-61 | -1.69E-46 |
| MS.gene058365 | MS.gene21037 | 0.839515 | 1.51E-57 | -1.69E-46 |
| MS.gene05842 | MS.gene21037 | 0.801123 | 1.04E-48 | -1.69E-46 |
| MS.gene058444 | MS.gene21037 | 0.82077 | 5.76E-53 | -1.69E-46 |
| MS.gene058660 | MS.gene21037 | 0.822209 | 2.68E-53 | -1.69E-46 |
| MS.gene058700 | MS.gene21037 | 0.829297 | 5.57E-55 | -1.69E-46 |
| MS.gene058710 | MS.gene21037 | 0.801646 | 8.16E-49 | -1.69E-46 |
| MS.gene058787 | MS.gene21037 | 0.827514 | 1.50E-54 | -1.69E-46 |
| MS.gene058928 | MS.gene21037 | 0.811223 | 7.80E-51 | -1.69E-46 |
| MS.gene058948 | MS.gene21037 | 0.842506 | 2.48E-58 | -1.69E-46 |
| MS.gene058949 | MS.gene21037 | 0.81542 | 9.33E-52 | -1.69E-46 |
| MS.gene058995 | MS.gene21037 | 0.826429 | 2.73E-54 | -1.69E-46 |
| MS.gene05907 | MS.gene21037 | 0.854468 | 1.21E-61 | -1.69E-46 |
| MS.gene059082 | MS.gene21037 | 0.802624 | 5.14E-49 | -1.69E-46 |
| MS.gene059294 | MS.gene21037 | 0.810785 | 9.70E-51 | -1.69E-46 |
| MS.gene059366 | MS.gene21037 | 0.826515 | 2.60E-54 | -1.69E-46 |
| MS.gene059420 | MS.gene21037 | 0.863956 | 1.72E-64 | -1.69E-46 |
| MS.gene059462 | MS.gene21037 | 0.807983 | 3.87E-50 | -1.69E-46 |
| MS.gene059518 | MS.gene21037 | 0.810651 | 1.04E-50 | -1.69E-46 |
| MS.gene059597 | MS.gene21037 | 0.815162 | 1.06E-51 | -1.69E-46 |
| MS.gene059674 | MS.gene21037 | 0.827513 | 1.50E-54 | -1.69E-46 |
| MS.gene059676 | MS.gene21037 | 0.846573 | 2.00E-59 | -1.69E-46 |
| MS.gene059688 | MS.gene21037 | 0.883813 | 3.26E-71 | -1.69E-46 |
| MS.gene059692 | MS.gene21037 | 0.893812 | 4.44E-75 | -1.69E-46 |
| MS.gene059695 | MS.gene21037 | 0.897211 | 1.75E-76 | -1.69E-46 |
| MS.gene059711 | MS.gene21037 | 0.830984 | 2.16E-55 | -1.69E-46 |
| MS.gene059807 | MS.gene21037 | 0.83653 | 8.86E-57 | -1.69E-46 |
| MS.gene05984 | MS.gene21037 | 0.821223 | 4.52E-53 | -1.69E-46 |
| MS.gene059876 | MS.gene21037 | 0.81488 | 1.23E-51 | -1.69E-46 |
| MS.gene059974 | MS.gene21037 | 0.848631 | 5.42E-60 | -1.69E-46 |
| MS.gene060193 | MS.gene21037 | 0.813064 | 3.09E-51 | -1.69E-46 |
| MS.gene06037 | MS.gene21037 | 0.850415 | 1.72E-60 | -1.69E-46 |
| MS.gene060403 | MS.gene21037 | 0.862775 | 3.99E-64 | -1.69E-46 |
| MS.gene060469 | MS.gene21037 | 0.82188 | 3.19E-53 | -1.69E-46 |
| MS.gene060569 | MS.gene21037 | 0.857076 | 2.09E-62 | -1.69E-46 |
| MS.gene060804 | MS.gene21037 | 0.828695 | 7.79E-55 | -1.69E-46 |
| MS.gene060835 | MS.gene21037 | 0.804118 | 2.52E-49 | -1.69E-46 |
| MS.gene060837 | MS.gene21037 | 0.876881 | 9.81E-69 | -1.69E-46 |
| MS.gene060986 | MS.gene21037 | 0.839052 | 2.00E-57 | -1.69E-46 |
| MS.gene061049 | MS.gene21037 | 0.856377 | 3.35E-62 | -1.69E-46 |
| MS.gene061051 | MS.gene21037 | -0.831281 | 1.82E-55 | -1.69E-46 |
| MS.gene061154 | MS.gene21037 | 0.86781 | 1.04E-65 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene21037.t1 | MTR_1g084200 | 98.266 | 173 | 2 | 1 | 1 | 172 | 1 | 173 | 4.56e-110 | 311 |
| MS.gene21037.t1 | MTR_7g111900 | 65.143 | 175 | 53 | 2 | 1 | 172 | 1 | 170 | 5.80e-74 | 219 |
| MS.gene21037.t1 | MTR_7g103200 | 67.516 | 157 | 46 | 1 | 1 | 157 | 1 | 152 | 3.08e-72 | 214 |
| MS.gene21037.t1 | MTR_2g019480 | 48.062 | 129 | 67 | 0 | 12 | 140 | 16 | 144 | 2.34e-41 | 136 |
| MS.gene21037.t1 | MTR_4g122930 | 48.299 | 147 | 71 | 3 | 1 | 145 | 1 | 144 | 5.09e-39 | 130 |
| MS.gene21037.t1 | MTR_2g046150 | 45.926 | 135 | 72 | 1 | 3 | 137 | 7 | 140 | 5.83e-37 | 125 |
| MS.gene21037.t1 | MTR_2g091215 | 46.512 | 129 | 69 | 0 | 9 | 137 | 4 | 132 | 6.02e-31 | 109 |
| MS.gene21037.t1 | MTR_2g091210 | 41.270 | 126 | 74 | 0 | 14 | 139 | 9 | 134 | 1.33e-30 | 108 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene21037.t1 | AT1G56580 | 60.241 | 166 | 65 | 1 | 1 | 165 | 1 | 166 | 1.93e-65 | 198 |
| MS.gene21037.t1 | AT1G09310 | 57.303 | 178 | 68 | 2 | 1 | 171 | 1 | 177 | 2.25e-64 | 196 |
| MS.gene21037.t1 | AT5G46230 | 43.846 | 130 | 73 | 0 | 9 | 138 | 11 | 140 | 7.46e-38 | 127 |
| MS.gene21037.t1 | AT4G24130 | 41.549 | 142 | 83 | 0 | 9 | 150 | 13 | 154 | 4.24e-37 | 125 |
| MS.gene21037.t1 | AT5G49600 | 44.444 | 153 | 74 | 4 | 3 | 145 | 1 | 152 | 7.69e-34 | 117 |
| MS.gene21037.t1 | AT1G30020 | 47.959 | 98 | 51 | 0 | 13 | 110 | 15 | 112 | 2.76e-30 | 108 |
Find 42 sgRNAs with CRISPR-Local
Find 47 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTATGTCCTTCAAAGGTAAA+AGG | 0.309948 | 1.4:+68809800 | None:intergenic |
| TGAGATAGAAGGAGAAGTTA+AGG | 0.310033 | 1.4:-68809500 | MS.gene21037:CDS |
| TCCATTGTATACCTCTGATT+TGG | 0.345924 | 1.4:+68809882 | None:intergenic |
| GTTGATAGGCTTGTGACTTA+TGG | 0.355562 | 1.4:-68809700 | MS.gene21037:CDS |
| ATCACATTCAGGGCTATTAC+AGG | 0.368505 | 1.4:-68809553 | MS.gene21037:CDS |
| CAACTGGTAAAATCACATTC+AGG | 0.368809 | 1.4:-68809564 | MS.gene21037:CDS |
| TAGAAGAATGTGGATATCAT+AGG | 0.386766 | 1.4:-68809780 | MS.gene21037:CDS |
| ACAAGTTTGAGAAAGTTGAT+AGG | 0.420157 | 1.4:-68809714 | MS.gene21037:CDS |
| GGTAAGATCAAGAAGCTAAC+TGG | 0.432806 | 1.4:-68809649 | MS.gene21037:CDS |
| TATTTCCAAGAGAAGTTCCT+TGG | 0.437882 | 1.4:+68809837 | None:intergenic |
| AGGTGAAGGAGCTCTTGGTT+TGG | 0.449978 | 1.4:-68809621 | MS.gene21037:CDS |
| AAAGTCCAAGGAACTTCTCT+TGG | 0.460248 | 1.4:-68809842 | MS.gene21037:CDS |
| GAAGTTCCTTGGACTTTGCT+TGG | 0.463735 | 1.4:+68809848 | None:intergenic |
| ACAGCAGCAGCAGTAGCAGC+AGG | 0.477244 | 1.4:+68809433 | None:intergenic |
| CAGTTGGTGGATCATCCAAC+TGG | 0.480486 | 1.4:+68809581 | None:intergenic |
| TTCAAAGGTAAAAGGCCATT+GGG | 0.484390 | 1.4:+68809808 | None:intergenic |
| TGTGAAGGTGAAGGAGCTCT+TGG | 0.487405 | 1.4:-68809626 | MS.gene21037:CDS |
| TCTATCTCAAAAGCTGAGGC+AGG | 0.495029 | 1.4:+68809514 | None:intergenic |
| CAGTTGGATGATCCACCAAC+TGG | 0.513052 | 1.4:-68809580 | MS.gene21037:CDS |
| CTTCAAAGGTAAAAGGCCAT+TGG | 0.515656 | 1.4:+68809807 | None:intergenic |
| AGGAGAAGTTAAGGATGTGA+AGG | 0.516179 | 1.4:-68809491 | MS.gene21037:CDS |
| CATTCTTCTATGTCCTTCAA+AGG | 0.524774 | 1.4:+68809793 | None:intergenic |
| AACTGGTAAAATCACATTCA+GGG | 0.526250 | 1.4:-68809563 | MS.gene21037:CDS |
| TGTGGATATCATAGGGAGAG+TGG | 0.541120 | 1.4:-68809772 | MS.gene21037:CDS |
| AAGGTAAAAGGCCATTGGGT+AGG | 0.553037 | 1.4:+68809812 | None:intergenic |
| AGAAGAATGTGGATATCATA+GGG | 0.557026 | 1.4:-68809779 | MS.gene21037:CDS |
| TCCACTGCATGAAATCCAGT+TGG | 0.581202 | 1.4:-68809596 | MS.gene21037:CDS |
| CAAGAAGCTAACTGGTGTGA+AGG | 0.582814 | 1.4:-68809641 | MS.gene21037:CDS |
| TGATTTGGCTCTGATCTCAT+CGG | 0.588078 | 1.4:+68809897 | None:intergenic |
| GCTAACTGGTGTGAAGGTGA+AGG | 0.589102 | 1.4:-68809635 | MS.gene21037:CDS |
| TCCAACTGGATTTCATGCAG+TGG | 0.601299 | 1.4:+68809595 | None:intergenic |
| TGCTGCTGTTGAAGTCAAAG+AGG | 0.615559 | 1.4:-68809419 | MS.gene21037:CDS |
| GCCAAATCAGAGGTATACAA+TGG | 0.617305 | 1.4:-68809883 | MS.gene21037:CDS |
| TGAAGTTACTGCCACTGTTG+AGG | 0.621958 | 1.4:-68809674 | MS.gene21037:CDS |
| CTATCTCAAAAGCTGAGGCA+GGG | 0.624154 | 1.4:+68809515 | None:intergenic |
| TCCTTCTATCTCAAAAGCTG+AGG | 0.629108 | 1.4:+68809510 | None:intergenic |
| TTGAAGGACATAGAAGAATG+TGG | 0.634606 | 1.4:-68809790 | MS.gene21037:CDS |
| GTTACTGCCACTGTTGAGGT+TGG | 0.637022 | 1.4:-68809670 | MS.gene21037:CDS |
| TTGGAAATAAACCTACCCAA+TGG | 0.663914 | 1.4:-68809823 | MS.gene21037:CDS |
| AATCTGCCAAGCAAAGTCCA+AGG | 0.673023 | 1.4:-68809854 | MS.gene21037:CDS |
| TGAGATCAGAGCCAAATCAG+AGG | 0.680456 | 1.4:-68809893 | MS.gene21037:CDS |
| GATCTTACCAACCTCAACAG+TGG | 0.683396 | 1.4:+68809663 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AACTGGTAAAATCACATTCA+GGG | - | chr1.4:68809761-68809780 | MS.gene21037:CDS | 30.0% | |
| AGAAGAATGTGGATATCATA+GGG | - | chr1.4:68809545-68809564 | MS.gene21037:CDS | 30.0% | |
| TAGAAGAATGTGGATATCAT+AGG | - | chr1.4:68809544-68809563 | MS.gene21037:CDS | 30.0% | |
| ! | ACAAGTTTGAGAAAGTTGAT+AGG | - | chr1.4:68809610-68809629 | MS.gene21037:CDS | 30.0% |
| CAACTGGTAAAATCACATTC+AGG | - | chr1.4:68809760-68809779 | MS.gene21037:CDS | 35.0% | |
| CATTCTTCTATGTCCTTCAA+AGG | + | chr1.4:68809534-68809553 | None:intergenic | 35.0% | |
| CTATGTCCTTCAAAGGTAAA+AGG | + | chr1.4:68809527-68809546 | None:intergenic | 35.0% | |
| TATTTCCAAGAGAAGTTCCT+TGG | + | chr1.4:68809490-68809509 | None:intergenic | 35.0% | |
| TCCATTGTATACCTCTGATT+TGG | + | chr1.4:68809445-68809464 | None:intergenic | 35.0% | |
| TGAGATAGAAGGAGAAGTTA+AGG | - | chr1.4:68809824-68809843 | MS.gene21037:CDS | 35.0% | |
| TTCAAAGGTAAAAGGCCATT+GGG | + | chr1.4:68809519-68809538 | None:intergenic | 35.0% | |
| TTGAAGGACATAGAAGAATG+TGG | - | chr1.4:68809534-68809553 | MS.gene21037:CDS | 35.0% | |
| TTGGAAATAAACCTACCCAA+TGG | - | chr1.4:68809501-68809520 | MS.gene21037:CDS | 35.0% | |
| ! | AATGTGATTTTACCAGTTGG+TGG | + | chr1.4:68809759-68809778 | None:intergenic | 35.0% |
| ! | CTGAATGTGATTTTACCAGT+TGG | + | chr1.4:68809762-68809781 | None:intergenic | 35.0% |
| AAAGTCCAAGGAACTTCTCT+TGG | - | chr1.4:68809482-68809501 | MS.gene21037:CDS | 40.0% | |
| AGGAGAAGTTAAGGATGTGA+AGG | - | chr1.4:68809833-68809852 | MS.gene21037:CDS | 40.0% | |
| ATCACATTCAGGGCTATTAC+AGG | - | chr1.4:68809771-68809790 | MS.gene21037:CDS | 40.0% | |
| CTTCAAAGGTAAAAGGCCAT+TGG | + | chr1.4:68809520-68809539 | None:intergenic | 40.0% | |
| GGTAAGATCAAGAAGCTAAC+TGG | - | chr1.4:68809675-68809694 | MS.gene21037:CDS | 40.0% | |
| TCCTTCTATCTCAAAAGCTG+AGG | + | chr1.4:68809817-68809836 | None:intergenic | 40.0% | |
| ! | CAATGGCCTTTTACCTTTGA+AGG | - | chr1.4:68809518-68809537 | MS.gene21037:CDS | 40.0% |
| ! | GCCAAATCAGAGGTATACAA+TGG | - | chr1.4:68809441-68809460 | MS.gene21037:CDS | 40.0% |
| ! | GTTGATAGGCTTGTGACTTA+TGG | - | chr1.4:68809624-68809643 | MS.gene21037:CDS | 40.0% |
| !! | TGATTTGGCTCTGATCTCAT+CGG | + | chr1.4:68809430-68809449 | None:intergenic | 40.0% |
| !!! | ATAGGGAGAGTGGTTTTGTT+TGG | - | chr1.4:68809562-68809581 | MS.gene21037:CDS | 40.0% |
| AAGGTAAAAGGCCATTGGGT+AGG | + | chr1.4:68809515-68809534 | None:intergenic | 45.0% | |
| AATCTGCCAAGCAAAGTCCA+AGG | - | chr1.4:68809470-68809489 | MS.gene21037:CDS | 45.0% | |
| CAAGAAGCTAACTGGTGTGA+AGG | - | chr1.4:68809683-68809702 | MS.gene21037:CDS | 45.0% | |
| CTATCTCAAAAGCTGAGGCA+GGG | + | chr1.4:68809812-68809831 | None:intergenic | 45.0% | |
| GAAGTTCCTTGGACTTTGCT+TGG | + | chr1.4:68809479-68809498 | None:intergenic | 45.0% | |
| GATCTTACCAACCTCAACAG+TGG | + | chr1.4:68809664-68809683 | None:intergenic | 45.0% | |
| TCCAACTGGATTTCATGCAG+TGG | + | chr1.4:68809732-68809751 | None:intergenic | 45.0% | |
| TCCACTGCATGAAATCCAGT+TGG | - | chr1.4:68809728-68809747 | MS.gene21037:CDS | 45.0% | |
| TCTATCTCAAAAGCTGAGGC+AGG | + | chr1.4:68809813-68809832 | None:intergenic | 45.0% | |
| TGAAGTTACTGCCACTGTTG+AGG | - | chr1.4:68809650-68809669 | MS.gene21037:CDS | 45.0% | |
| TGAGATCAGAGCCAAATCAG+AGG | - | chr1.4:68809431-68809450 | MS.gene21037:CDS | 45.0% | |
| TGCTGCTGTTGAAGTCAAAG+AGG | - | chr1.4:68809905-68809924 | MS.gene21037:CDS | 45.0% | |
| TGTGGATATCATAGGGAGAG+TGG | - | chr1.4:68809552-68809571 | MS.gene21037:CDS | 45.0% | |
| !!! | GCCTCAGCTTTTGAGATAGA+AGG | - | chr1.4:68809813-68809832 | MS.gene21037:CDS | 45.0% |
| AGGTGAAGGAGCTCTTGGTT+TGG | - | chr1.4:68809703-68809722 | MS.gene21037:CDS | 50.0% | |
| CAGTTGGATGATCCACCAAC+TGG | - | chr1.4:68809744-68809763 | MS.gene21037:CDS | 50.0% | |
| GCTAACTGGTGTGAAGGTGA+AGG | - | chr1.4:68809689-68809708 | MS.gene21037:CDS | 50.0% | |
| GTTACTGCCACTGTTGAGGT+TGG | - | chr1.4:68809654-68809673 | MS.gene21037:CDS | 50.0% | |
| TGTGAAGGTGAAGGAGCTCT+TGG | - | chr1.4:68809698-68809717 | MS.gene21037:CDS | 50.0% | |
| !! | CAGTTGGTGGATCATCCAAC+TGG | + | chr1.4:68809746-68809765 | None:intergenic | 50.0% |
| ACAGCAGCAGCAGTAGCAGC+AGG | + | chr1.4:68809894-68809913 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.4 | gene | 68809414 | 68809932 | 68809414 | ID=MS.gene21037 |
| chr1.4 | mRNA | 68809414 | 68809932 | 68809414 | ID=MS.gene21037.t1;Parent=MS.gene21037 |
| chr1.4 | exon | 68809414 | 68809932 | 68809414 | ID=MS.gene21037.t1.exon1;Parent=MS.gene21037.t1 |
| chr1.4 | CDS | 68809414 | 68809932 | 68809414 | ID=cds.MS.gene21037.t1;Parent=MS.gene21037.t1 |
| Gene Sequence |
| Protein sequence |
