Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene35751.t1 | XP_003597086.1 | 99.6 | 229 | 1 | 0 | 1 | 229 | 1 | 229 | 2.30E-129 | 471.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene35751.t1 | P26291 | 93.5 | 230 | 14 | 1 | 1 | 229 | 1 | 230 | 7.2e-124 | 444.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene35751.t1 | B7FGU7 | 99.6 | 229 | 1 | 0 | 1 | 229 | 1 | 229 | 1.6e-129 | 471.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049171 | MS.gene35751 | 0.801912 | 7.20E-49 | -1.69E-46 |
| MS.gene049173 | MS.gene35751 | 0.80534 | 1.40E-49 | -1.69E-46 |
| MS.gene049217 | MS.gene35751 | 0.809134 | 2.20E-50 | -1.69E-46 |
| MS.gene049222 | MS.gene35751 | 0.827201 | 1.78E-54 | -1.69E-46 |
| MS.gene049285 | MS.gene35751 | 0.85606 | 4.15E-62 | -1.69E-46 |
| MS.gene049320 | MS.gene35751 | 0.852816 | 3.60E-61 | -1.69E-46 |
| MS.gene049369 | MS.gene35751 | 0.819504 | 1.12E-52 | -1.69E-46 |
| MS.gene049427 | MS.gene35751 | 0.850612 | 1.52E-60 | -1.69E-46 |
| MS.gene049609 | MS.gene35751 | 0.855023 | 8.33E-62 | -1.69E-46 |
| MS.gene049614 | MS.gene35751 | 0.847904 | 8.61E-60 | -1.69E-46 |
| MS.gene049633 | MS.gene35751 | 0.829007 | 6.55E-55 | -1.69E-46 |
| MS.gene049805 | MS.gene35751 | 0.851975 | 6.25E-61 | -1.69E-46 |
| MS.gene050163 | MS.gene35751 | 0.840631 | 7.74E-58 | -1.69E-46 |
| MS.gene050165 | MS.gene35751 | 0.828391 | 9.23E-55 | -1.69E-46 |
| MS.gene050333 | MS.gene35751 | 0.83455 | 2.81E-56 | -1.69E-46 |
| MS.gene050504 | MS.gene35751 | 0.814361 | 1.60E-51 | -1.69E-46 |
| MS.gene050518 | MS.gene35751 | 0.823696 | 1.21E-53 | -1.69E-46 |
| MS.gene050593 | MS.gene35751 | 0.846977 | 1.55E-59 | -1.69E-46 |
| MS.gene050612 | MS.gene35751 | 0.82813 | 1.07E-54 | -1.69E-46 |
| MS.gene050675 | MS.gene35751 | 0.841623 | 4.25E-58 | -1.69E-46 |
| MS.gene050699 | MS.gene35751 | 0.828138 | 1.06E-54 | -1.69E-46 |
| MS.gene050747 | MS.gene35751 | 0.828601 | 8.21E-55 | -1.69E-46 |
| MS.gene050767 | MS.gene35751 | 0.833295 | 5.79E-56 | -1.69E-46 |
| MS.gene050770 | MS.gene35751 | 0.836139 | 1.11E-56 | -1.69E-46 |
| MS.gene050772 | MS.gene35751 | 0.878242 | 3.29E-69 | -1.69E-46 |
| MS.gene050950 | MS.gene35751 | 0.845045 | 5.19E-59 | -1.69E-46 |
| MS.gene050986 | MS.gene35751 | 0.829883 | 4.01E-55 | -1.69E-46 |
| MS.gene05100 | MS.gene35751 | 0.805209 | 1.49E-49 | -1.69E-46 |
| MS.gene05105 | MS.gene35751 | 0.831413 | 1.69E-55 | -1.69E-46 |
| MS.gene051228 | MS.gene35751 | 0.836115 | 1.13E-56 | -1.69E-46 |
| MS.gene051387 | MS.gene35751 | 0.805935 | 1.05E-49 | -1.69E-46 |
| MS.gene051551 | MS.gene35751 | 0.814984 | 1.17E-51 | -1.69E-46 |
| MS.gene051617 | MS.gene35751 | 0.819025 | 1.44E-52 | -1.69E-46 |
| MS.gene051810 | MS.gene35751 | 0.88287 | 7.25E-71 | -1.69E-46 |
| MS.gene051858 | MS.gene35751 | 0.819081 | 1.40E-52 | -1.69E-46 |
| MS.gene052271 | MS.gene35751 | 0.883405 | 4.62E-71 | -1.69E-46 |
| MS.gene052290 | MS.gene35751 | 0.804678 | 1.93E-49 | -1.69E-46 |
| MS.gene052310 | MS.gene35751 | 0.814371 | 1.59E-51 | -1.69E-46 |
| MS.gene052379 | MS.gene35751 | 0.854141 | 1.50E-61 | -1.69E-46 |
| MS.gene052421 | MS.gene35751 | 0.836371 | 9.73E-57 | -1.69E-46 |
| MS.gene052484 | MS.gene35751 | 0.805797 | 1.12E-49 | -1.69E-46 |
| MS.gene052615 | MS.gene35751 | 0.88389 | 3.06E-71 | -1.69E-46 |
| MS.gene052870 | MS.gene35751 | 0.826165 | 3.15E-54 | -1.69E-46 |
| MS.gene052872 | MS.gene35751 | 0.823944 | 1.05E-53 | -1.69E-46 |
| MS.gene052916 | MS.gene35751 | 0.826427 | 2.73E-54 | -1.69E-46 |
| MS.gene053009 | MS.gene35751 | 0.826742 | 2.30E-54 | -1.69E-46 |
| MS.gene053103 | MS.gene35751 | 0.80074 | 1.25E-48 | -1.69E-46 |
| MS.gene053104 | MS.gene35751 | 0.819762 | 9.80E-53 | -1.69E-46 |
| MS.gene053207 | MS.gene35751 | 0.811809 | 5.82E-51 | -1.69E-46 |
| MS.gene053213 | MS.gene35751 | 0.876076 | 1.86E-68 | -1.69E-46 |
| MS.gene053286 | MS.gene35751 | 0.869864 | 2.25E-66 | -1.69E-46 |
| MS.gene053322 | MS.gene35751 | 0.853211 | 2.78E-61 | -1.69E-46 |
| MS.gene053384 | MS.gene35751 | 0.800817 | 1.21E-48 | -1.69E-46 |
| MS.gene053449 | MS.gene35751 | 0.813983 | 1.94E-51 | -1.69E-46 |
| MS.gene053574 | MS.gene35751 | 0.866484 | 2.76E-65 | -1.69E-46 |
| MS.gene053632 | MS.gene35751 | 0.801585 | 8.40E-49 | -1.69E-46 |
| MS.gene053813 | MS.gene35751 | 0.810164 | 1.32E-50 | -1.69E-46 |
| MS.gene053912 | MS.gene35751 | 0.833493 | 5.17E-56 | -1.69E-46 |
| MS.gene054376 | MS.gene35751 | 0.819431 | 1.17E-52 | -1.69E-46 |
| MS.gene054408 | MS.gene35751 | 0.836561 | 8.71E-57 | -1.69E-46 |
| MS.gene054600 | MS.gene35751 | 0.819391 | 1.19E-52 | -1.69E-46 |
| MS.gene054642 | MS.gene35751 | 0.858005 | 1.11E-62 | -1.69E-46 |
| MS.gene054954 | MS.gene35751 | 0.828283 | 9.80E-55 | -1.69E-46 |
| MS.gene05505 | MS.gene35751 | 0.849239 | 3.67E-60 | -1.69E-46 |
| MS.gene055206 | MS.gene35751 | 0.811529 | 6.69E-51 | -1.69E-46 |
| MS.gene055212 | MS.gene35751 | 0.807847 | 4.14E-50 | -1.69E-46 |
| MS.gene055362 | MS.gene35751 | 0.825896 | 3.65E-54 | -1.69E-46 |
| MS.gene055397 | MS.gene35751 | 0.841741 | 3.95E-58 | -1.69E-46 |
| MS.gene055412 | MS.gene35751 | 0.829965 | 3.83E-55 | -1.69E-46 |
| MS.gene055499 | MS.gene35751 | 0.856343 | 3.43E-62 | -1.69E-46 |
| MS.gene055502 | MS.gene35751 | 0.841305 | 5.15E-58 | -1.69E-46 |
| MS.gene055636 | MS.gene35751 | 0.82233 | 2.51E-53 | -1.69E-46 |
| MS.gene055647 | MS.gene35751 | 0.854531 | 1.16E-61 | -1.69E-46 |
| MS.gene055722 | MS.gene35751 | 0.860256 | 2.35E-63 | -1.69E-46 |
| MS.gene05592 | MS.gene35751 | 0.858599 | 7.37E-63 | -1.69E-46 |
| MS.gene055941 | MS.gene35751 | 0.818073 | 2.37E-52 | -1.69E-46 |
| MS.gene056083 | MS.gene35751 | 0.817041 | 4.05E-52 | -1.69E-46 |
| MS.gene056374 | MS.gene35751 | 0.845994 | 2.87E-59 | -1.69E-46 |
| MS.gene056422 | MS.gene35751 | 0.806621 | 7.53E-50 | -1.69E-46 |
| MS.gene056510 | MS.gene35751 | 0.81728 | 3.58E-52 | -1.69E-46 |
| MS.gene056511 | MS.gene35751 | 0.811502 | 6.78E-51 | -1.69E-46 |
| MS.gene056549 | MS.gene35751 | 0.818869 | 1.56E-52 | -1.69E-46 |
| MS.gene056609 | MS.gene35751 | 0.843675 | 1.21E-58 | -1.69E-46 |
| MS.gene056657 | MS.gene35751 | 0.865746 | 4.72E-65 | -1.69E-46 |
| MS.gene056897 | MS.gene35751 | 0.850954 | 1.22E-60 | -1.69E-46 |
| MS.gene057011 | MS.gene35751 | 0.829182 | 5.94E-55 | -1.69E-46 |
| MS.gene057076 | MS.gene35751 | 0.865567 | 5.38E-65 | -1.69E-46 |
| MS.gene057129 | MS.gene35751 | 0.840745 | 7.23E-58 | -1.69E-46 |
| MS.gene057146 | MS.gene35751 | 0.818703 | 1.71E-52 | -1.69E-46 |
| MS.gene057331 | MS.gene35751 | 0.873541 | 1.36E-67 | -1.69E-46 |
| MS.gene057416 | MS.gene35751 | 0.86483 | 9.17E-65 | -1.69E-46 |
| MS.gene058019 | MS.gene35751 | 0.821704 | 3.50E-53 | -1.69E-46 |
| MS.gene058130 | MS.gene35751 | 0.856903 | 2.35E-62 | -1.69E-46 |
| MS.gene058323 | MS.gene35751 | 0.813202 | 2.88E-51 | -1.69E-46 |
| MS.gene05837 | MS.gene35751 | 0.808603 | 2.86E-50 | -1.69E-46 |
| MS.gene058419 | MS.gene35751 | 0.856144 | 3.92E-62 | -1.69E-46 |
| MS.gene058544 | MS.gene35751 | 0.825028 | 5.86E-54 | -1.69E-46 |
| MS.gene058723 | MS.gene35751 | 0.825974 | 3.50E-54 | -1.69E-46 |
| MS.gene058773 | MS.gene35751 | 0.821692 | 3.53E-53 | -1.69E-46 |
| MS.gene05883 | MS.gene35751 | 0.828629 | 8.08E-55 | -1.69E-46 |
| MS.gene058928 | MS.gene35751 | 0.801772 | 7.69E-49 | -1.69E-46 |
| MS.gene059022 | MS.gene35751 | 0.834511 | 2.87E-56 | -1.69E-46 |
| MS.gene059023 | MS.gene35751 | 0.80204 | 6.77E-49 | -1.69E-46 |
| MS.gene059029 | MS.gene35751 | 0.85289 | 3.43E-61 | -1.69E-46 |
| MS.gene05913 | MS.gene35751 | 0.814158 | 1.78E-51 | -1.69E-46 |
| MS.gene059305 | MS.gene35751 | 0.840398 | 8.91E-58 | -1.69E-46 |
| MS.gene05934 | MS.gene35751 | 0.806335 | 8.65E-50 | -1.69E-46 |
| MS.gene059536 | MS.gene35751 | 0.855382 | 6.55E-62 | -1.69E-46 |
| MS.gene059677 | MS.gene35751 | 0.840493 | 8.41E-58 | -1.69E-46 |
| MS.gene059688 | MS.gene35751 | 0.830543 | 2.77E-55 | -1.69E-46 |
| MS.gene059692 | MS.gene35751 | 0.825258 | 5.17E-54 | -1.69E-46 |
| MS.gene059695 | MS.gene35751 | 0.823616 | 1.26E-53 | -1.69E-46 |
| MS.gene059711 | MS.gene35751 | 0.820767 | 5.76E-53 | -1.69E-46 |
| MS.gene059718 | MS.gene35751 | 0.830698 | 2.54E-55 | -1.69E-46 |
| MS.gene059756 | MS.gene35751 | 0.801752 | 7.76E-49 | -1.69E-46 |
| MS.gene059858 | MS.gene35751 | 0.811907 | 5.54E-51 | -1.69E-46 |
| MS.gene059865 | MS.gene35751 | 0.830371 | 3.05E-55 | -1.69E-46 |
| MS.gene059902 | MS.gene35751 | 0.814595 | 1.42E-51 | -1.69E-46 |
| MS.gene059960 | MS.gene35751 | 0.849145 | 3.90E-60 | -1.69E-46 |
| MS.gene060762 | MS.gene35751 | 0.807932 | 3.97E-50 | -1.69E-46 |
| MS.gene060804 | MS.gene35751 | 0.847446 | 1.15E-59 | -1.69E-46 |
| MS.gene060818 | MS.gene35751 | 0.845863 | 3.11E-59 | -1.69E-46 |
| MS.gene060835 | MS.gene35751 | 0.815413 | 9.36E-52 | -1.69E-46 |
| MS.gene061041 | MS.gene35751 | 0.836965 | 6.87E-57 | -1.69E-46 |
| MS.gene061134 | MS.gene35751 | 0.841447 | 4.73E-58 | -1.69E-46 |
| MS.gene061150 | MS.gene35751 | 0.821252 | 4.46E-53 | -1.69E-46 |
| MS.gene061200 | MS.gene35751 | 0.853046 | 3.09E-61 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene35751.t1 | MTR_2g089510 | 99.563 | 229 | 1 | 0 | 1 | 229 | 1 | 229 | 9.73e-169 | 464 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene35751.t1 | AT4G03280 | 75.000 | 232 | 52 | 4 | 1 | 229 | 1 | 229 | 1.05e-108 | 312 |
| MS.gene35751.t1 | AT4G03280 | 78.365 | 208 | 41 | 3 | 25 | 229 | 4 | 210 | 1.21e-102 | 296 |
Find 80 sgRNAs with CRISPR-Local
Find 147 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTCTTCTCAGCTTGATTAAA+TGG | 0.193267 | 2.1:+12605102 | None:intergenic |
| ACCTTGAATCTTCTTCTTCT+TGG | 0.209001 | 2.1:-12606285 | MS.gene35751:CDS |
| GCTGGAATGCTTGTTCCTTA+TGG | 0.229044 | 2.1:-12606243 | MS.gene35751:CDS |
| TACTCTTACACAAGGATTAA+AGG | 0.280573 | 2.1:-12605423 | MS.gene35751:intron |
| CCAATCATCTGAGTCCTTGT+TGG | 0.323325 | 2.1:+12606387 | None:intergenic |
| GATACCTGAGAAGGGGTTGC+AGG | 0.386192 | 2.1:+12606556 | None:intergenic |
| GCTCTTTCACTTCCTACTGC+TGG | 0.406855 | 2.1:-12606261 | MS.gene35751:CDS |
| TGTGAGAGGACCTGCTCCTT+TGG | 0.407122 | 2.1:-12605031 | MS.gene35751:intron |
| GGAATTTCATGTCCATCAAT+AGG | 0.407782 | 2.1:-12606423 | MS.gene35751:CDS |
| GGATCCTTCCTTGTTCCACC+AGG | 0.427870 | 2.1:-12606222 | MS.gene35751:CDS |
| ATCCCCTGCAACCCCTTCTC+AGG | 0.428243 | 2.1:-12606560 | MS.gene35751:intron |
| GGAAGGTTGTGTTTGTTCCA+TGG | 0.434059 | 2.1:-12604366 | MS.gene35751:CDS |
| GTTGAAACAGATTTCAGAAC+AGG | 0.439951 | 2.1:-12604343 | MS.gene35751:CDS |
| GTCTCTCTCTTACTCATGTC+AGG | 0.452312 | 2.1:+12606306 | None:intergenic |
| GTCCTCTCACAACTCTTCCT+TGG | 0.456035 | 2.1:+12605043 | None:intergenic |
| CTGAAATCTGTTTCAACCCA+TGG | 0.465352 | 2.1:+12604349 | None:intergenic |
| ACCAAGAAGAAGAAGATTCA+AGG | 0.470226 | 2.1:+12606284 | None:intergenic |
| AGCATCACTTGCCAGGCTAC+AGG | 0.471775 | 2.1:-12606348 | MS.gene35751:CDS |
| TCAGATGATTGGTAAAGGTA+AGG | 0.479300 | 2.1:-12606376 | MS.gene35751:CDS |
| GGGAATGAGCATCACTTGCC+AGG | 0.491362 | 2.1:-12606355 | MS.gene35751:CDS |
| CAGCAGGAATGCCTGTAGCC+TGG | 0.498499 | 2.1:+12606337 | None:intergenic |
| GAAGGTTGTGTTTGTTCCAT+GGG | 0.499364 | 2.1:-12604365 | MS.gene35751:CDS |
| ATGCCGTATGCACTCACCTC+GGG | 0.502003 | 2.1:-12605134 | MS.gene35751:CDS |
| ATTGTACTGAGATCCGTGGC+AGG | 0.506893 | 2.1:+12605068 | None:intergenic |
| AATTTGCTTACCAAAGGAGC+AGG | 0.507050 | 2.1:+12605021 | None:intergenic |
| TACAGCTCAGGTTCTTCCAC+TGG | 0.509786 | 2.1:-12605524 | MS.gene35751:intron |
| AGGGGTTGCAGGGGATAGAG+TGG | 0.510029 | 2.1:+12606567 | None:intergenic |
| TTCACAAGTAGTCCTATTGA+TGG | 0.511244 | 2.1:+12606411 | None:intergenic |
| TTAATCCTTGTGTAAGAGTA+CGG | 0.511377 | 2.1:+12605426 | None:intergenic |
| ACACCCGAGGTGAGTGCATA+CGG | 0.514765 | 2.1:+12605131 | None:intergenic |
| TTGTGTAAGAGTACGGTCAC+CGG | 0.527077 | 2.1:+12605433 | None:intergenic |
| CCAACAAGGACTCAGATGAT+TGG | 0.533278 | 2.1:-12606387 | MS.gene35751:CDS |
| CAGATGATTGGTAAAGGTAA+GGG | 0.543592 | 2.1:-12606375 | MS.gene35751:CDS |
| CACTGGTGGTACTGTAGCAA+AGG | 0.547649 | 2.1:-12605507 | MS.gene35751:CDS |
| GATCCTTCCTTGTTCCACCA+GGG | 0.557310 | 2.1:-12606221 | MS.gene35751:intron |
| ATACCTGAGAAGGGGTTGCA+GGG | 0.561697 | 2.1:+12606557 | None:intergenic |
| TGTTGGACAGTCTCTTGCGT+TGG | 0.563000 | 2.1:-12604416 | MS.gene35751:intron |
| ATGTCAGGTACACGATCAGC+AGG | 0.563729 | 2.1:+12606321 | None:intergenic |
| CAGCTATGTTCTGGAAAGAG+TGG | 0.564531 | 2.1:-12606444 | MS.gene35751:CDS |
| GGATAGAGTGGTGGAAGACA+TGG | 0.564996 | 2.1:+12606579 | None:intergenic |
| GTGATGTTAGTGTGGATGAT+GGG | 0.565796 | 2.1:-12604387 | MS.gene35751:CDS |
| AAGTTCATCTGCCCCTGCCA+CGG | 0.566507 | 2.1:-12605081 | MS.gene35751:CDS |
| ATGATGTAGTTGCAGCGGAA+TGG | 0.567006 | 2.1:-12605472 | MS.gene35751:CDS |
| TGTACTGAGATCCGTGGCAG+GGG | 0.568736 | 2.1:+12605070 | None:intergenic |
| AGCTCAGGTTCTTCCACTGG+TGG | 0.569111 | 2.1:-12605521 | MS.gene35751:CDS |
| AGGACTCAGATGATTGGTAA+AGG | 0.572119 | 2.1:-12606381 | MS.gene35751:CDS |
| TGTGATGTTAGTGTGGATGA+TGG | 0.576215 | 2.1:-12604388 | MS.gene35751:CDS |
| TGTGTAAGAGTACGGTCACC+GGG | 0.581349 | 2.1:+12605434 | None:intergenic |
| TGGCTAAAGTCACATGGACC+CGG | 0.583593 | 2.1:-12605452 | MS.gene35751:CDS |
| TAACTAACCCTGGTGGAACA+AGG | 0.585258 | 2.1:+12606214 | None:intergenic |
| TCAGAACAGGTGATGCTCCA+TGG | 0.585443 | 2.1:-12604330 | MS.gene35751:CDS |
| ACTGTAGCAAAGGATGCCGT+TGG | 0.586270 | 2.1:-12605497 | MS.gene35751:CDS |
| GGAACAAGGAAGGATCCATA+AGG | 0.588651 | 2.1:+12606228 | None:intergenic |
| GGAACAAGCATTCCAGCAGT+AGG | 0.593273 | 2.1:+12606249 | None:intergenic |
| CTCTCTACCACTAGGTAGGT+AGG | 0.599627 | 2.1:+12605186 | None:intergenic |
| TTGTACTGAGATCCGTGGCA+GGG | 0.599883 | 2.1:+12605069 | None:intergenic |
| TGTTAGTGTGGATGATGGGA+AGG | 0.602477 | 2.1:-12604383 | MS.gene35751:CDS |
| TTTGCAATGATACCTGAGAA+GGG | 0.603738 | 2.1:+12606548 | None:intergenic |
| GACTACTTGTGAAGCCAACA+AGG | 0.607551 | 2.1:-12606401 | MS.gene35751:CDS |
| TTGCAATGATACCTGAGAAG+GGG | 0.608443 | 2.1:+12606549 | None:intergenic |
| TGCAACTACATCATTGCCAA+CGG | 0.609037 | 2.1:+12605481 | None:intergenic |
| GGTTGCAGGGGATAGAGTGG+TGG | 0.611449 | 2.1:+12606570 | None:intergenic |
| TAACCCTGGTGGAACAAGGA+AGG | 0.611521 | 2.1:+12606218 | None:intergenic |
| AATGCCGTATGCACTCACCT+CGG | 0.615718 | 2.1:-12605135 | MS.gene35751:CDS |
| TACCTGAGAAGGGGTTGCAG+GGG | 0.643153 | 2.1:+12606558 | None:intergenic |
| TGGCAATGATGTAGTTGCAG+CGG | 0.645737 | 2.1:-12605477 | MS.gene35751:CDS |
| GACCAAGGAAGAGTTGTGAG+AGG | 0.654345 | 2.1:-12605045 | MS.gene35751:CDS |
| GGCACATTGTGATGTTAGTG+TGG | 0.657647 | 2.1:-12604395 | MS.gene35751:CDS |
| CTTTGCTACAGTACCACCAG+TGG | 0.660969 | 2.1:+12605508 | None:intergenic |
| GCGGAATGGCTAAAGTCACA+TGG | 0.661778 | 2.1:-12605458 | MS.gene35751:CDS |
| AATAAAATAACTAACCCTGG+TGG | 0.665632 | 2.1:+12606207 | None:intergenic |
| TTCTGTCACTCTCTACCACT+AGG | 0.667971 | 2.1:+12605178 | None:intergenic |
| GTCACTCTCTACCACTAGGT+AGG | 0.672164 | 2.1:+12605182 | None:intergenic |
| GGTCATTGTACTGAGATCCG+TGG | 0.688553 | 2.1:+12605064 | None:intergenic |
| TAAGAAGTCTAAGACCACCA+TGG | 0.706343 | 2.1:+12604313 | None:intergenic |
| GGTGACCGTACTCTTACACA+AGG | 0.707111 | 2.1:-12605431 | MS.gene35751:CDS |
| GAACAGGTGATGCTCCATGG+TGG | 0.711317 | 2.1:-12604327 | MS.gene35751:CDS |
| GGATCTCAGTACAATGACCA+AGG | 0.711476 | 2.1:-12605060 | MS.gene35751:CDS |
| TAAATGGCACGACACACCCG+AGG | 0.714387 | 2.1:+12605118 | None:intergenic |
| GGGTGATCCTACCTACCTAG+TGG | 0.715843 | 2.1:-12605193 | MS.gene35751:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTATTTATTTATTTATTTT+TGG | - | chr2.1:12604428-12604447 | MS.gene35751:intron | 0.0% |
| !!! | AACAAATTTATTTATTTTTC+TGG | + | chr2.1:12606363-12606382 | None:intergenic | 10.0% |
| !! | AATAAGATGAAAATAGTTTA+TGG | + | chr2.1:12606086-12606105 | None:intergenic | 15.0% |
| !! | ATTTATCAAAGTAGTTATTA+TGG | + | chr2.1:12605676-12605695 | None:intergenic | 15.0% |
| !!! | AAATAAATTTGTTTAGTGAT+AGG | - | chr2.1:12606370-12606389 | MS.gene35751:CDS | 15.0% |
| !!! | TTAAGGTTTTATTCTATATT+TGG | - | chr2.1:12604804-12604823 | MS.gene35751:intron | 15.0% |
| !! | ACTTTGATAAATTCAATAAC+AGG | - | chr2.1:12605684-12605703 | MS.gene35751:intron | 20.0% |
| !! | ATAAATAAAATAACTAACCC+TGG | + | chr2.1:12604697-12604716 | None:intergenic | 20.0% |
| !! | CTTTGATAAATTCAATAACA+GGG | - | chr2.1:12605685-12605704 | MS.gene35751:intron | 20.0% |
| !! | GGAATATTAATGAACATTAT+TGG | - | chr2.1:12606453-12606472 | MS.gene35751:intron | 20.0% |
| !! | TAATTAAATTGTTCAGTGAT+AGG | - | chr2.1:12606228-12606247 | MS.gene35751:CDS | 20.0% |
| !!! | CTTTTTATGATTTGTTTCTT+TGG | - | chr2.1:12605972-12605991 | MS.gene35751:intron | 20.0% |
| !!! | TTAAAGCATCATAAAACATT+TGG | + | chr2.1:12605306-12605325 | None:intergenic | 20.0% |
| ! | AGTGTAATGTTGTATAGTAT+AGG | - | chr2.1:12604895-12604914 | MS.gene35751:intron | 25.0% |
| ! | CTTATGAAAACGACTTATAA+TGG | + | chr2.1:12605534-12605553 | None:intergenic | 25.0% |
| ! | GCAACATATAATTAGCAATA+TGG | + | chr2.1:12605274-12605293 | None:intergenic | 25.0% |
| ! | TAGCTATAAATTACCTCTTA+AGG | + | chr2.1:12606327-12606346 | None:intergenic | 25.0% |
| ! | TATCAAAGTAGTTATTATGG+TGG | + | chr2.1:12605673-12605692 | None:intergenic | 25.0% |
| ! | TATTTCTTGTGTTTACATGT+CGG | + | chr2.1:12604961-12604980 | None:intergenic | 25.0% |
| !!! | AACATTATTGGTCTTTATGT+TGG | - | chr2.1:12606465-12606484 | MS.gene35751:intron | 25.0% |
| !!! | CATGTTTTTAGCTCAAATTA+AGG | - | chr2.1:12604787-12604806 | MS.gene35751:intron | 25.0% |
| AATAAAATAACTAACCCTGG+TGG | + | chr2.1:12604694-12604713 | None:intergenic | 30.0% | |
| AGAGGTAATTTATAGCTACT+AGG | - | chr2.1:12606329-12606348 | MS.gene35751:CDS | 30.0% | |
| AGTGTATAGCAAATAACCAT+CGG | - | chr2.1:12605133-12605152 | MS.gene35751:CDS | 30.0% | |
| ATCAAATCATTAACCAGTCT+CGG | - | chr2.1:12605343-12605362 | MS.gene35751:intron | 30.0% | |
| GATACATGACTACATGAATT+AGG | + | chr2.1:12605183-12605202 | None:intergenic | 30.0% | |
| TAAGCACTTAAGATACTGTT+TGG | - | chr2.1:12605618-12605637 | MS.gene35751:intron | 30.0% | |
| TACTCTTACACAAGGATTAA+AGG | - | chr2.1:12605475-12605494 | MS.gene35751:CDS | 30.0% | |
| TTAATCCTTGTGTAAGAGTA+CGG | + | chr2.1:12605475-12605494 | None:intergenic | 30.0% | |
| TTCTTCTCAGCTTGATTAAA+TGG | + | chr2.1:12605799-12605818 | None:intergenic | 30.0% | |
| TTTAGTGATAGGATAGCTTA+GGG | - | chr2.1:12606381-12606400 | MS.gene35751:CDS | 30.0% | |
| ! | AACTAGTTTGTCAAGTGATA+AGG | + | chr2.1:12605909-12605928 | None:intergenic | 30.0% |
| ! | ATTTGTTTCTTTGGTCTGTT+TGG | - | chr2.1:12605981-12606000 | MS.gene35751:intron | 30.0% |
| ! | CAATTTGAAATCGTGTTCAA+GGG | + | chr2.1:12605062-12605081 | None:intergenic | 30.0% |
| ! | TCAATTTGAAATCGTGTTCA+AGG | + | chr2.1:12605063-12605082 | None:intergenic | 30.0% |
| !! | CTTATTTCTATATGCTCTCT+AGG | - | chr2.1:12606100-12606119 | MS.gene35751:intron | 30.0% |
| !! | TTTTCCAATTTGCTTACCAA+AGG | + | chr2.1:12605886-12605905 | None:intergenic | 30.0% |
| ACCAAGAAGAAGAAGATTCA+AGG | + | chr2.1:12604617-12604636 | None:intergenic | 35.0% | |
| ACCTCTTAAGGTGAATAAGT+GGG | + | chr2.1:12606315-12606334 | None:intergenic | 35.0% | |
| ACCTTGAATCTTCTTCTTCT+TGG | - | chr2.1:12604613-12604632 | MS.gene35751:intron | 35.0% | |
| ATGCATCATGTAATTGTTGC+TGG | - | chr2.1:12606432-12606451 | MS.gene35751:CDS | 35.0% | |
| CAATATCTAGCAGACTAGTA+TGG | + | chr2.1:12605091-12605110 | None:intergenic | 35.0% | |
| CAGATGATTGGTAAAGGTAA+GGG | - | chr2.1:12604523-12604542 | MS.gene35751:intron | 35.0% | |
| GACGTTGCATAATATATGCA+GGG | - | chr2.1:12606279-12606298 | MS.gene35751:CDS | 35.0% | |
| GGAATTTCATGTCCATCAAT+AGG | - | chr2.1:12604475-12604494 | MS.gene35751:intron | 35.0% | |
| GTTGAAACAGATTTCAGAAC+AGG | - | chr2.1:12606555-12606574 | MS.gene35751:intron | 35.0% | |
| GTTTAGTGATAGGATAGCTT+AGG | - | chr2.1:12606380-12606399 | MS.gene35751:CDS | 35.0% | |
| TACCAACTAAGTTAAGCTCA+CGG | + | chr2.1:12606257-12606276 | None:intergenic | 35.0% | |
| TACCTCTTAAGGTGAATAAG+TGG | + | chr2.1:12606316-12606335 | None:intergenic | 35.0% | |
| TCAGATGATTGGTAAAGGTA+AGG | - | chr2.1:12604522-12604541 | MS.gene35751:intron | 35.0% | |
| TCTTGTCTCATTAGCATCAA+AGG | - | chr2.1:12605215-12605234 | MS.gene35751:intron | 35.0% | |
| TTCACAAGTAGTCCTATTGA+TGG | + | chr2.1:12604490-12604509 | None:intergenic | 35.0% | |
| TTGTGTTTACATGTCGGTAT+CGG | + | chr2.1:12604955-12604974 | None:intergenic | 35.0% | |
| TTTGCAATGATACCTGAGAA+GGG | + | chr2.1:12604353-12604372 | None:intergenic | 35.0% | |
| ! | TGAGCTTAACTTAGTTGGTA+GGG | - | chr2.1:12606257-12606276 | MS.gene35751:CDS | 35.0% |
| ! | TTTTGCAATGATACCTGAGA+AGG | + | chr2.1:12604354-12604373 | None:intergenic | 35.0% |
| !!! | TTTTGGTTTCAGCTATGTTC+TGG | - | chr2.1:12604445-12604464 | MS.gene35751:intron | 35.0% |
| ACCAACTAAGTTAAGCTCAC+GGG | + | chr2.1:12606256-12606275 | None:intergenic | 40.0% | |
| AGGACTCAGATGATTGGTAA+AGG | - | chr2.1:12604517-12604536 | MS.gene35751:intron | 40.0% | |
| ATAGTACAATCGAAAGCCGA+TGG | + | chr2.1:12605152-12605171 | None:intergenic | 40.0% | |
| CCTCTTAAGGTGAATAAGTG+GGG | + | chr2.1:12606314-12606333 | None:intergenic | 40.0% | |
| CGACATAACTTCGATACATG+TGG | + | chr2.1:12605014-12605033 | None:intergenic | 40.0% | |
| CTAGGACAGCTTATAGCTTA+TGG | - | chr2.1:12606118-12606137 | MS.gene35751:intron | 40.0% | |
| CTGAAATCTGTTTCAACCCA+TGG | + | chr2.1:12606552-12606571 | None:intergenic | 40.0% | |
| GAAGGTTGTGTTTGTTCCAT+GGG | - | chr2.1:12606533-12606552 | MS.gene35751:intron | 40.0% | |
| GACTACATGAATTAGGTGCA+AGG | + | chr2.1:12605176-12605195 | None:intergenic | 40.0% | |
| GCAAGTGTACAATTGCAAAC+TGG | - | chr2.1:12605497-12605516 | MS.gene35751:CDS | 40.0% | |
| GGACGTTGCATAATATATGC+AGG | - | chr2.1:12606278-12606297 | MS.gene35751:CDS | 40.0% | |
| GTGAGCTTAACTTAGTTGGT+AGG | - | chr2.1:12606256-12606275 | MS.gene35751:CDS | 40.0% | |
| GTGATGTTAGTGTGGATGAT+GGG | - | chr2.1:12606511-12606530 | MS.gene35751:intron | 40.0% | |
| TCGGAAAACAATTACAGCTC+AGG | - | chr2.1:12605362-12605381 | MS.gene35751:intron | 40.0% | |
| TGCAACTACATCATTGCCAA+CGG | + | chr2.1:12605420-12605439 | None:intergenic | 40.0% | |
| TGCTCCTTTGGTAAGCAAAT+TGG | - | chr2.1:12605879-12605898 | MS.gene35751:intron | 40.0% | |
| TGTGATGTTAGTGTGGATGA+TGG | - | chr2.1:12606510-12606529 | MS.gene35751:intron | 40.0% | |
| TTGCAATGATACCTGAGAAG+GGG | + | chr2.1:12604352-12604371 | None:intergenic | 40.0% | |
| TTGCATAGACACACTGTTTG+AGG | - | chr2.1:12606029-12606048 | MS.gene35751:intron | 40.0% | |
| ! | AATTTGCTTACCAAAGGAGC+AGG | + | chr2.1:12605880-12605899 | None:intergenic | 40.0% |
| ! | ATTATGGTGGAAGCTTGAGA+AGG | + | chr2.1:12605660-12605679 | None:intergenic | 40.0% |
| ! | CTGTAATTGTTTTCCGAGAC+TGG | + | chr2.1:12605359-12605378 | None:intergenic | 40.0% |
| ! | TGACAAACTAGTTTAGCTGC+AGG | - | chr2.1:12605916-12605935 | MS.gene35751:intron | 40.0% |
| ATGATGTAGTTGCAGCGGAA+TGG | - | chr2.1:12605426-12605445 | MS.gene35751:CDS | 45.0% | |
| CCAACAAGGACTCAGATGAT+TGG | - | chr2.1:12604511-12604530 | MS.gene35751:intron | 45.0% | |
| CCAACTAAGTTAAGCTCACG+GGG | + | chr2.1:12606255-12606274 | None:intergenic | 45.0% | |
| CCAATCATCTGAGTCCTTGT+TGG | + | chr2.1:12604514-12604533 | None:intergenic | 45.0% | |
| CCCCACTTATTCACCTTAAG+AGG | - | chr2.1:12606311-12606330 | MS.gene35751:CDS | 45.0% | |
| GACTACTTGTGAAGCCAACA+AGG | - | chr2.1:12604497-12604516 | MS.gene35751:intron | 45.0% | |
| GCTGGAATGCTTGTTCCTTA+TGG | - | chr2.1:12604655-12604674 | MS.gene35751:intron | 45.0% | |
| GGAACAAGGAAGGATCCATA+AGG | + | chr2.1:12604673-12604692 | None:intergenic | 45.0% | |
| GGAAGGTTGTGTTTGTTCCA+TGG | - | chr2.1:12606532-12606551 | MS.gene35751:intron | 45.0% | |
| GGATCTCAGTACAATGACCA+AGG | - | chr2.1:12605838-12605857 | MS.gene35751:intron | 45.0% | |
| GGCACATTGTGATGTTAGTG+TGG | - | chr2.1:12606503-12606522 | MS.gene35751:intron | 45.0% | |
| GTCTCTCTCTTACTCATGTC+AGG | + | chr2.1:12604595-12604614 | None:intergenic | 45.0% | |
| TAACTAACCCTGGTGGAACA+AGG | + | chr2.1:12604687-12604706 | None:intergenic | 45.0% | |
| TGGCAATGATGTAGTTGCAG+CGG | - | chr2.1:12605421-12605440 | MS.gene35751:intron | 45.0% | |
| TTCTGTCACTCTCTACCACT+AGG | + | chr2.1:12605723-12605742 | None:intergenic | 45.0% | |
| ! | TTGTGTAAGAGTACGGTCAC+CGG | + | chr2.1:12605468-12605487 | None:intergenic | 45.0% |
| !! | CAGCTATGTTCTGGAAAGAG+TGG | - | chr2.1:12604454-12604473 | MS.gene35751:intron | 45.0% |
| !! | TGTTAGTGTGGATGATGGGA+AGG | - | chr2.1:12606515-12606534 | MS.gene35751:intron | 45.0% |
| !!! | ATTTTTAATATCTAATTTAA+AGG | - | chr2.1:12604383-12604402 | MS.gene35751:CDS | 5.0% |
| !!! | TTTTTAATATCTAATTTAAA+GGG | - | chr2.1:12604384-12604403 | MS.gene35751:CDS | 5.0% |
| AATGCCGTATGCACTCACCT+CGG | - | chr2.1:12605763-12605782 | MS.gene35751:intron | 50.0% | |
| ACTGTAGCAAAGGATGCCGT+TGG | - | chr2.1:12605401-12605420 | MS.gene35751:intron | 50.0% | |
| AGGTGAATAAGTGGGGTGTG+CGG | + | chr2.1:12606307-12606326 | None:intergenic | 50.0% | |
| ATACCTGAGAAGGGGTTGCA+GGG | + | chr2.1:12604344-12604363 | None:intergenic | 50.0% | |
| ATCGTGTTCAAGGGCTCGAT+AGG | + | chr2.1:12605053-12605072 | None:intergenic | 50.0% | |
| ATGTCAGGTACACGATCAGC+AGG | + | chr2.1:12604580-12604599 | None:intergenic | 50.0% | |
| ATTGTACTGAGATCCGTGGC+AGG | + | chr2.1:12605833-12605852 | None:intergenic | 50.0% | |
| CACTGGTGGTACTGTAGCAA+AGG | - | chr2.1:12605391-12605410 | MS.gene35751:intron | 50.0% | |
| CCCCGTGAGCTTAACTTAGT+TGG | - | chr2.1:12606252-12606271 | MS.gene35751:CDS | 50.0% | |
| CTCTCTACCACTAGGTAGGT+AGG | + | chr2.1:12605715-12605734 | None:intergenic | 50.0% | |
| CTTTGCTACAGTACCACCAG+TGG | + | chr2.1:12605393-12605412 | None:intergenic | 50.0% | |
| GACCAAGGAAGAGTTGTGAG+AGG | - | chr2.1:12605853-12605872 | MS.gene35751:intron | 50.0% | |
| GATCCTTCCTTGTTCCACCA+GGG | - | chr2.1:12604677-12604696 | MS.gene35751:intron | 50.0% | |
| GCGGAATGGCTAAAGTCACA+TGG | - | chr2.1:12605440-12605459 | MS.gene35751:CDS | 50.0% | |
| GCTCTTTCACTTCCTACTGC+TGG | - | chr2.1:12604637-12604656 | MS.gene35751:intron | 50.0% | |
| GGAACAAGCATTCCAGCAGT+AGG | + | chr2.1:12604652-12604671 | None:intergenic | 50.0% | |
| GGTCATTGTACTGAGATCCG+TGG | + | chr2.1:12605837-12605856 | None:intergenic | 50.0% | |
| GGTGACCGTACTCTTACACA+AGG | - | chr2.1:12605467-12605486 | MS.gene35751:CDS | 50.0% | |
| GTCACTCTCTACCACTAGGT+AGG | + | chr2.1:12605719-12605738 | None:intergenic | 50.0% | |
| GTCCTCTCACAACTCTTCCT+TGG | + | chr2.1:12605858-12605877 | None:intergenic | 50.0% | |
| TAACCCTGGTGGAACAAGGA+AGG | + | chr2.1:12604683-12604702 | None:intergenic | 50.0% | |
| TACAGCTCAGGTTCTTCCAC+TGG | - | chr2.1:12605374-12605393 | MS.gene35751:intron | 50.0% | |
| TCAGAACAGGTGATGCTCCA+TGG | - | chr2.1:12606568-12606587 | MS.gene35751:CDS | 50.0% | |
| TGGCTAAAGTCACATGGACC+CGG | - | chr2.1:12605446-12605465 | MS.gene35751:CDS | 50.0% | |
| TGTTGGACAGTCTCTTGCGT+TGG | - | chr2.1:12606482-12606501 | MS.gene35751:intron | 50.0% | |
| TTGTACTGAGATCCGTGGCA+GGG | + | chr2.1:12605832-12605851 | None:intergenic | 50.0% | |
| ! | TGTGTAAGAGTACGGTCACC+GGG | + | chr2.1:12605467-12605486 | None:intergenic | 50.0% |
| AAGTTCATCTGCCCCTGCCA+CGG | - | chr2.1:12605817-12605836 | MS.gene35751:intron | 55.0% | |
| ACACCCGAGGTGAGTGCATA+CGG | + | chr2.1:12605770-12605789 | None:intergenic | 55.0% | |
| AGCATCACTTGCCAGGCTAC+AGG | - | chr2.1:12604550-12604569 | MS.gene35751:intron | 55.0% | |
| AGCTCAGGTTCTTCCACTGG+TGG | - | chr2.1:12605377-12605396 | MS.gene35751:intron | 55.0% | |
| ATGCCGTATGCACTCACCTC+GGG | - | chr2.1:12605764-12605783 | MS.gene35751:intron | 55.0% | |
| GAACAGGTGATGCTCCATGG+TGG | - | chr2.1:12606571-12606590 | MS.gene35751:CDS | 55.0% | |
| GATACCTGAGAAGGGGTTGC+AGG | + | chr2.1:12604345-12604364 | None:intergenic | 55.0% | |
| GGATCCTTCCTTGTTCCACC+AGG | - | chr2.1:12604676-12604695 | MS.gene35751:intron | 55.0% | |
| GGGAATGAGCATCACTTGCC+AGG | - | chr2.1:12604543-12604562 | MS.gene35751:intron | 55.0% | |
| GGGTGATCCTACCTACCTAG+TGG | - | chr2.1:12605705-12605724 | MS.gene35751:intron | 55.0% | |
| GGTGAATAAGTGGGGTGTGC+GGG | + | chr2.1:12606306-12606325 | None:intergenic | 55.0% | |
| GTGAATAAGTGGGGTGTGCG+GGG | + | chr2.1:12606305-12606324 | None:intergenic | 55.0% | |
| TAAATGGCACGACACACCCG+AGG | + | chr2.1:12605783-12605802 | None:intergenic | 55.0% | |
| TACCTGAGAAGGGGTTGCAG+GGG | + | chr2.1:12604343-12604362 | None:intergenic | 55.0% | |
| TGTACTGAGATCCGTGGCAG+GGG | + | chr2.1:12605831-12605850 | None:intergenic | 55.0% | |
| TGTGAGAGGACCTGCTCCTT+TGG | - | chr2.1:12605867-12605886 | MS.gene35751:intron | 55.0% | |
| AGGGGTTGCAGGGGATAGAG+TGG | + | chr2.1:12604334-12604353 | None:intergenic | 60.0% | |
| ATCCCCTGCAACCCCTTCTC+AGG | - | chr2.1:12604338-12604357 | MS.gene35751:CDS | 60.0% | |
| CAGCAGGAATGCCTGTAGCC+TGG | + | chr2.1:12604564-12604583 | None:intergenic | 60.0% | |
| GGTTGCAGGGGATAGAGTGG+TGG | + | chr2.1:12604331-12604350 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.1 | gene | 12604321 | 12606599 | 12604321 | ID=MS.gene35751 |
| chr2.1 | mRNA | 12604321 | 12606599 | 12604321 | ID=MS.gene35751.t1;Parent=MS.gene35751 |
| chr2.1 | exon | 12606561 | 12606599 | 12606561 | ID=MS.gene35751.t1.exon1;Parent=MS.gene35751.t1 |
| chr2.1 | CDS | 12606561 | 12606599 | 12606561 | ID=cds.MS.gene35751.t1;Parent=MS.gene35751.t1 |
| chr2.1 | exon | 12606222 | 12606463 | 12606222 | ID=MS.gene35751.t1.exon2;Parent=MS.gene35751.t1 |
| chr2.1 | CDS | 12606222 | 12606463 | 12606222 | ID=cds.MS.gene35751.t1;Parent=MS.gene35751.t1 |
| chr2.1 | exon | 12605424 | 12605541 | 12605424 | ID=MS.gene35751.t1.exon3;Parent=MS.gene35751.t1 |
| chr2.1 | CDS | 12605424 | 12605541 | 12605424 | ID=cds.MS.gene35751.t1;Parent=MS.gene35751.t1 |
| chr2.1 | exon | 12605032 | 12605214 | 12605032 | ID=MS.gene35751.t1.exon4;Parent=MS.gene35751.t1 |
| chr2.1 | CDS | 12605032 | 12605214 | 12605032 | ID=cds.MS.gene35751.t1;Parent=MS.gene35751.t1 |
| chr2.1 | exon | 12604321 | 12604428 | 12604321 | ID=MS.gene35751.t1.exon5;Parent=MS.gene35751.t1 |
| chr2.1 | CDS | 12604321 | 12604428 | 12604321 | ID=cds.MS.gene35751.t1;Parent=MS.gene35751.t1 |
| Gene Sequence |
| Protein sequence |
