AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene49010


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene49010.t1 MTR_7g056413 95.122 205 10 0 1 205 1 205 9.16e-91 265
MS.gene49010.t1 MTR_2g026670 43.825 251 93 8 1 204 1 250 2.55e-45 150
MS.gene49010.t1 MTR_4g133900 79.167 72 15 0 4 75 8 79 3.29e-32 117
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene49010.t1 AT4G16380 65.333 75 26 0 2 76 13 87 1.04e-29 110
MS.gene49010.t1 AT4G16380 65.333 75 26 0 2 76 6 80 1.20e-29 110
MS.gene49010.t1 AT1G51090 35.784 204 97 5 1 204 1 170 1.13e-23 92.8
MS.gene49010.t1 AT1G49420 49.367 79 39 1 2 79 6 84 1.13e-18 80.5
MS.gene49010.t1 AT4G16380 62.222 45 17 0 32 76 15 59 5.31e-14 68.6

Find 67 sgRNAs with CRISPR-Local

Find 94 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATATTGTCTCTTACCTTCTT+TGG 0.194931 7.3:+48495891 None:intergenic
CATTCTTCTCATCATATATT+TGG 0.199717 7.3:+48495946 None:intergenic
GTTCCTTATGGGCATGTTTA+TGG 0.209138 7.3:-48495591 MS.gene49010:CDS
CCTTCGCCAGTTGTTCCTTA+TGG 0.209497 7.3:-48495603 MS.gene49010:CDS
CTTCGCCAGTTGTTCCTTAT+GGG 0.271859 7.3:-48495602 MS.gene49010:CDS
AACTATTTCTATGCTCTTGA+TGG 0.278008 7.3:+48495848 None:intergenic
TATTGTCTCTTACCTTCTTT+GGG 0.283371 7.3:+48495892 None:intergenic
CACAAGGACCCTTCAACTTA+TGG 0.333356 7.3:+48495443 None:intergenic
GCCGGAGCTGGTGCCGGAGC+TGG 0.340628 7.3:+48495708 None:intergenic
ATCGGCTTTGCCTCGGCCAC+GGG 0.356925 7.3:+48495771 None:intergenic
TCATGTGCTGGCTTAGGCTT+TGG 0.357337 7.3:+48495819 None:intergenic
CCATCCATAACTATCATATA+CGG 0.359961 7.3:+48495530 None:intergenic
GCCGGAGCTGGAGCTGGACT+TGG 0.364027 7.3:+48495720 None:intergenic
GGCGGGCCAGGTTGTGCAAT+CGG 0.376262 7.3:+48495753 None:intergenic
GCTGGTGCCGGAGCTGGAGC+TGG 0.380110 7.3:+48495714 None:intergenic
GCTGCAGCACACCACTCTTA+TGG 0.386416 7.3:+48495914 None:intergenic
GCCGGTGCCTGAGCCGGAGC+TGG 0.401075 7.3:+48495696 None:intergenic
AATCGGCTTTGCCTCGGCCA+CGG 0.401999 7.3:+48495770 None:intergenic
TCTTTGGGCTTCTCATGTGC+TGG 0.402264 7.3:+48495807 None:intergenic
GCCTGAGCCGGAGCTGGTGC+CGG 0.417776 7.3:+48495702 None:intergenic
TATGGGCATGTTTATGGTGC+TGG 0.421138 7.3:-48495585 MS.gene49010:CDS
TCCAGCTCCGGCACCAGCTC+CGG 0.434194 7.3:-48495709 MS.gene49010:CDS
TCCGGCACCAGCTCCGGCTC+AGG 0.434564 7.3:-48495703 MS.gene49010:CDS
ATGGAGTGGGCCTTGTTATG+TGG 0.439002 7.3:-48495511 MS.gene49010:CDS
ACATGCCCATAAGGAACAAC+TGG 0.446939 7.3:+48495597 None:intergenic
GGTGCAGTCGCAGAAGGAAG+TGG 0.461054 7.3:+48495672 None:intergenic
CATGTTTATGGTGCTGGACA+AGG 0.462627 7.3:-48495579 MS.gene49010:CDS
GGCTTCTCATGTGCTGGCTT+AGG 0.467240 7.3:+48495813 None:intergenic
TGTGCTGGCTTAGGCTTTGG+TGG 0.473606 7.3:+48495822 None:intergenic
AGTGGAGCCGGTGCCTGAGC+CGG 0.473677 7.3:+48495690 None:intergenic
TCTCTAAGCCATGGAGCAAA+AGG 0.474451 7.3:-48496522 None:intergenic
CGATTGCTTGCGCGAAGAAG+AGG 0.475381 7.3:-48495478 MS.gene49010:CDS
GAGACAATATTTGTTGTCAA+GGG 0.480293 7.3:-48495878 MS.gene49010:CDS
ACCAGCTCCGGCTCAGGCAC+CGG 0.483282 7.3:-48495697 MS.gene49010:CDS
GCACCATAAACATGCCCATA+AGG 0.487789 7.3:+48495588 None:intergenic
TTGTGCAATCGGCTTTGCCT+CGG 0.490744 7.3:+48495764 None:intergenic
TGGAGTGGGCCTTGTTATGT+GGG 0.498721 7.3:-48495510 MS.gene49010:CDS
GTCGCAGAAGGAAGTGGAGC+CGG 0.501463 7.3:+48495678 None:intergenic
TCCAAGTCCAGCTCCAGCTC+CGG 0.508493 7.3:-48495721 MS.gene49010:CDS
CGACATGTACCATAAGTTGA+AGG 0.510579 7.3:-48495452 None:intergenic
GCAAAGCCGATTGCACAACC+TGG 0.511042 7.3:-48495759 MS.gene49010:CDS
AGAGACAATATTTGTTGTCA+AGG 0.511179 7.3:-48495879 MS.gene49010:CDS
CAAATATATGATGAGAAGAA+TGG 0.524975 7.3:-48495945 MS.gene49010:CDS
CTTCTTCGCGCAAGCAATCG+TGG 0.538333 7.3:+48495481 None:intergenic
AGGCCCGTATATGATAGTTA+TGG 0.539945 7.3:-48495534 MS.gene49010:CDS
GGTGTGCTGCAGCCCAAAGA+AGG 0.550575 7.3:-48495904 MS.gene49010:CDS
ACAGCTGGTGCAGTCGCAGA+AGG 0.558520 7.3:+48495666 None:intergenic
CATCCATAACTATCATATAC+GGG 0.559099 7.3:+48495531 None:intergenic
TGGCGAAGGATATGCAAGAA+TGG 0.564765 7.3:+48495617 None:intergenic
ATGATAGTTATGGATGGAGT+GGG 0.567519 7.3:-48495524 MS.gene49010:CDS
TGGTGATGACCCACATAACA+AGG 0.583828 7.3:+48495501 None:intergenic
TTATGCAGGTTGCTATAATG+AGG 0.585675 7.3:-48496341 MS.gene49010:intron
TATGATAGTTATGGATGGAG+TGG 0.593289 7.3:-48495525 MS.gene49010:CDS
AGACAATATTTGTTGTCAAG+GGG 0.594817 7.3:-48495877 MS.gene49010:CDS
GCCTCCCGTAAAACTGTGGG+TGG 0.601345 7.3:+48495553 None:intergenic
ACGGGCCTCCCGTAAAACTG+TGG 0.609547 7.3:+48495549 None:intergenic
GTGTGAGGATACATTACAGC+TGG 0.610753 7.3:+48495651 None:intergenic
CCATAAGGAACAACTGGCGA+AGG 0.625985 7.3:+48495603 None:intergenic
GGTTGCTATAATGAGGCTCA+AGG 0.630973 7.3:-48496334 MS.gene49010:CDS
GACAATATTTGTTGTCAAGG+GGG 0.632032 7.3:-48495876 MS.gene49010:CDS
CCGTATATGATAGTTATGGA+TGG 0.637657 7.3:-48495530 MS.gene49010:CDS
ACTCACGAGGGTATTTGCAA+AGG 0.638434 7.3:+48496268 None:intergenic
CGGGCCTCCCGTAAAACTGT+GGG 0.643742 7.3:+48495550 None:intergenic
GGATATGCAAGAATGGACAT+TGG 0.650120 7.3:+48495624 None:intergenic
ATGGACATTGGCGTTGTGTG+AGG 0.663282 7.3:+48495636 None:intergenic
GTTTATGGTGCTGGACAAGG+TGG 0.674350 7.3:-48495576 MS.gene49010:CDS
TGGTATTGTGACCATAAGAG+TGG 0.707513 7.3:-48495925 MS.gene49010:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAATAAACAAATTAAGA+AGG + chr7.3:48495927-48495946 None:intergenic 10.0%
!! AAAATCAAGAAACTAAAATT+AGG + chr7.3:48495895-48495914 None:intergenic 15.0%
!!! AATTCAAGCATTTAATTTAA+TGG + chr7.3:48495578-48495597 None:intergenic 15.0%
!! GAAAGATAATAAAATATGAG+AGG + chr7.3:48495745-48495764 None:intergenic 20.0%
! CAAATATATGATGAGAAGAA+TGG - chr7.3:48496022-48496041 MS.gene49010:intron 25.0%
! CATTCTTCTCATCATATATT+TGG + chr7.3:48496024-48496043 None:intergenic 25.0%
! GCAATACTAACAAATTAAGA+AGG + chr7.3:48495956-48495975 None:intergenic 25.0%
! TGGTTATTACTAGAACTAAT+TGG + chr7.3:48495558-48495577 None:intergenic 25.0%
!! ATCTTTCCTCATACATATTT+TGG - chr7.3:48495758-48495777 MS.gene49010:CDS 25.0%
!! GGTGGTGTTAATATATTATT+AGG - chr7.3:48495784-48495803 MS.gene49010:CDS 25.0%
ACCATACCAAAATATGTATG+AGG + chr7.3:48495767-48495786 None:intergenic 30.0%
ATATTGTCTCTTACCTTCTT+TGG + chr7.3:48496079-48496098 None:intergenic 30.0%
CATCCATAACTATCATATAC+GGG + chr7.3:48496439-48496458 None:intergenic 30.0%
CCATCCATAACTATCATATA+CGG + chr7.3:48496440-48496459 None:intergenic 30.0%
TATTATTGAAAGACTCACGA+GGG + chr7.3:48495714-48495733 None:intergenic 30.0%
TATTGTCTCTTACCTTCTTT+GGG + chr7.3:48496078-48496097 None:intergenic 30.0%
TTATTATTGAAAGACTCACG+AGG + chr7.3:48495715-48495734 None:intergenic 30.0%
! AACTAATTGGCTTAGTTAAG+AGG + chr7.3:48495545-48495564 None:intergenic 30.0%
! TACAGCATTTTCTACATTGA+AGG + chr7.3:48495663-48495682 None:intergenic 30.0%
! TTCTTTTCTTGAACTTATGC+AGG - chr7.3:48495612-48495631 MS.gene49010:CDS 30.0%
!! AACTATTTCTATGCTCTTGA+TGG + chr7.3:48496122-48496141 None:intergenic 30.0%
!! AGACAATATTTGTTGTCAAG+GGG - chr7.3:48496090-48496109 MS.gene49010:intron 30.0%
!! AGAGACAATATTTGTTGTCA+AGG - chr7.3:48496088-48496107 MS.gene49010:intron 30.0%
!! GAGACAATATTTGTTGTCAA+GGG - chr7.3:48496089-48496108 MS.gene49010:intron 30.0%
!!! TCATACATATTTTGGTATGG+TGG - chr7.3:48495766-48495785 MS.gene49010:CDS 30.0%
!!! TCCTCATACATATTTTGGTA+TGG - chr7.3:48495763-48495782 MS.gene49010:CDS 30.0%
ATGATAGTTATGGATGGAGT+GGG - chr7.3:48496443-48496462 MS.gene49010:intron 35.0%
CCGTATATGATAGTTATGGA+TGG - chr7.3:48496437-48496456 MS.gene49010:intron 35.0%
TATGATAGTTATGGATGGAG+TGG - chr7.3:48496442-48496461 MS.gene49010:intron 35.0%
TTATGCAGGTTGCTATAATG+AGG - chr7.3:48495626-48495645 MS.gene49010:CDS 35.0%
! ATTGGCTTAGTTAAGAGGAA+TGG + chr7.3:48495540-48495559 None:intergenic 35.0%
!! GACAATATTTGTTGTCAAGG+GGG - chr7.3:48496091-48496110 MS.gene49010:intron 35.0%
!!! GGCTTTTTTTCTTGCTCTTT+GGG + chr7.3:48496178-48496197 None:intergenic 35.0%
AGGCCCGTATATGATAGTTA+TGG - chr7.3:48496433-48496452 MS.gene49010:intron 40.0%
GGATATGCAAGAATGGACAT+TGG + chr7.3:48496346-48496365 None:intergenic 40.0%
GTTCCTTATGGGCATGTTTA+TGG - chr7.3:48496376-48496395 MS.gene49010:intron 40.0%
TGGTATTGTGACCATAAGAG+TGG - chr7.3:48496042-48496061 MS.gene49010:intron 40.0%
!! AATATTTGTTGTCAAGGGGG+TGG - chr7.3:48496094-48496113 MS.gene49010:intron 40.0%
!! GACTTGGATTTTGTTTAGGC+GGG + chr7.3:48496234-48496253 None:intergenic 40.0%
!! GCTGGACTTGGATTTTGTTT+AGG + chr7.3:48496238-48496257 None:intergenic 40.0%
!! GGACTTGGATTTTGTTTAGG+CGG + chr7.3:48496235-48496254 None:intergenic 40.0%
!!! GGGCTTTTTTTCTTGCTCTT+TGG + chr7.3:48496179-48496198 None:intergenic 40.0%
ACATGCCCATAAGGAACAAC+TGG + chr7.3:48496373-48496392 None:intergenic 45.0%
AGAGCAAGAAAAAAAGCCCG+TGG - chr7.3:48496180-48496199 MS.gene49010:intron 45.0%
CTTCGCCAGTTGTTCCTTAT+GGG - chr7.3:48496365-48496384 MS.gene49010:intron 45.0%
GCACCATAAACATGCCCATA+AGG + chr7.3:48496382-48496401 None:intergenic 45.0%
GGTTGCTATAATGAGGCTCA+AGG - chr7.3:48495633-48495652 MS.gene49010:CDS 45.0%
GTGTGAGGATACATTACAGC+TGG + chr7.3:48496319-48496338 None:intergenic 45.0%
TATGGGCATGTTTATGGTGC+TGG - chr7.3:48496382-48496401 MS.gene49010:intron 45.0%
TGGCGAAGGATATGCAAGAA+TGG + chr7.3:48496353-48496372 None:intergenic 45.0%
! CATGTTTATGGTGCTGGACA+AGG - chr7.3:48496388-48496407 MS.gene49010:intron 45.0%
! TGGTGATGACCCACATAACA+AGG + chr7.3:48496469-48496488 None:intergenic 45.0%
!! ACTCACGAGGGTATTTGCAA+AGG + chr7.3:48495702-48495721 None:intergenic 45.0%
!! AATACAAAATTTATATTTAA+TGG + chr7.3:48495819-48495838 None:intergenic 5.0%
ATGGACATTGGCGTTGTGTG+AGG + chr7.3:48496334-48496353 None:intergenic 50.0%
CCATAAGGAACAACTGGCGA+AGG + chr7.3:48496367-48496386 None:intergenic 50.0%
CCTTCGCCAGTTGTTCCTTA+TGG - chr7.3:48496364-48496383 MS.gene49010:intron 50.0%
TCTTTGGGCTTCTCATGTGC+TGG + chr7.3:48496163-48496182 None:intergenic 50.0%
! GTTTATGGTGCTGGACAAGG+TGG - chr7.3:48496391-48496410 MS.gene49010:intron 50.0%
! TTGTGCAATCGGCTTTGCCT+CGG + chr7.3:48496206-48496225 None:intergenic 50.0%
!! ATGGAGTGGGCCTTGTTATG+TGG - chr7.3:48496456-48496475 MS.gene49010:intron 50.0%
!! TCATGTGCTGGCTTAGGCTT+TGG + chr7.3:48496151-48496170 None:intergenic 50.0%
!! TGGAGTGGGCCTTGTTATGT+GGG - chr7.3:48496457-48496476 MS.gene49010:intron 50.0%
AGAAAAAAAGCCCGTGGCCG+AGG - chr7.3:48496186-48496205 MS.gene49010:intron 55.0%
CGATTGCTTGCGCGAAGAAG+AGG - chr7.3:48496489-48496508 MS.gene49010:intron 55.0%
CTTCTTCGCGCAAGCAATCG+TGG + chr7.3:48496489-48496508 None:intergenic 55.0%
GCAAAGCCGATTGCACAACC+TGG - chr7.3:48496208-48496227 MS.gene49010:intron 55.0%
GCTGCAGCACACCACTCTTA+TGG + chr7.3:48496056-48496075 None:intergenic 55.0%
! GGTGGCCACCCACAGTTTTA+CGG - chr7.3:48496409-48496428 MS.gene49010:intron 55.0%
! GTGGCCACCCACAGTTTTAC+GGG - chr7.3:48496410-48496429 MS.gene49010:intron 55.0%
!! GGATTTTGTTTAGGCGGGCC+AGG + chr7.3:48496229-48496248 None:intergenic 55.0%
!! GGCTTCTCATGTGCTGGCTT+AGG + chr7.3:48496157-48496176 None:intergenic 55.0%
!! TGTGCTGGCTTAGGCTTTGG+TGG + chr7.3:48496148-48496167 None:intergenic 55.0%
ACAGCTGGTGCAGTCGCAGA+AGG + chr7.3:48496304-48496323 None:intergenic 60.0%
ACGGGCCTCCCGTAAAACTG+TGG + chr7.3:48496421-48496440 None:intergenic 60.0%
CGGGCCTCCCGTAAAACTGT+GGG + chr7.3:48496420-48496439 None:intergenic 60.0%
GCCTCCCGTAAAACTGTGGG+TGG + chr7.3:48496417-48496436 None:intergenic 60.0%
GGTGCAGTCGCAGAAGGAAG+TGG + chr7.3:48496298-48496317 None:intergenic 60.0%
GGTGTGCTGCAGCCCAAAGA+AGG - chr7.3:48496063-48496082 MS.gene49010:intron 60.0%
GTCGCAGAAGGAAGTGGAGC+CGG + chr7.3:48496292-48496311 None:intergenic 60.0%
TCCAAGTCCAGCTCCAGCTC+CGG - chr7.3:48496246-48496265 MS.gene49010:intron 60.0%
! AATCGGCTTTGCCTCGGCCA+CGG + chr7.3:48496200-48496219 None:intergenic 60.0%
! GCCACCCACAGTTTTACGGG+AGG - chr7.3:48496413-48496432 MS.gene49010:intron 60.0%
GGCGGGCCAGGTTGTGCAAT+CGG + chr7.3:48496217-48496236 None:intergenic 65.0%
! ATCGGCTTTGCCTCGGCCAC+GGG + chr7.3:48496199-48496218 None:intergenic 65.0%
ACCAGCTCCGGCTCAGGCAC+CGG - chr7.3:48496270-48496289 MS.gene49010:intron 70.0%
AGTGGAGCCGGTGCCTGAGC+CGG + chr7.3:48496280-48496299 None:intergenic 70.0%
GCCGGAGCTGGAGCTGGACT+TGG + chr7.3:48496250-48496269 None:intergenic 70.0%
TCCAGCTCCGGCACCAGCTC+CGG - chr7.3:48496258-48496277 MS.gene49010:intron 70.0%
GCCTGAGCCGGAGCTGGTGC+CGG + chr7.3:48496268-48496287 None:intergenic 75.0%
TCCGGCACCAGCTCCGGCTC+AGG - chr7.3:48496264-48496283 MS.gene49010:intron 75.0%
! GCTGGTGCCGGAGCTGGAGC+TGG + chr7.3:48496256-48496275 None:intergenic 75.0%
! GCCGGTGCCTGAGCCGGAGC+TGG + chr7.3:48496274-48496293 None:intergenic 80.0%
!! GCCGGAGCTGGTGCCGGAGC+TGG + chr7.3:48496262-48496281 None:intergenic 80.0%


Chromosome Type Strat End Strand Name
chr7.3 gene 48495455 48496534 48495455 ID=MS.gene49010
chr7.3 mRNA 48495455 48496534 48495455 ID=MS.gene49010.t1;Parent=MS.gene49010
chr7.3 exon 48496523 48496534 48496523 ID=MS.gene49010.t1.exon1;Parent=MS.gene49010.t1
chr7.3 CDS 48496523 48496534 48496523 ID=cds.MS.gene49010.t1;Parent=MS.gene49010.t1
chr7.3 exon 48496274 48496355 48496274 ID=MS.gene49010.t1.exon2;Parent=MS.gene49010.t1
chr7.3 CDS 48496274 48496355 48496274 ID=cds.MS.gene49010.t1;Parent=MS.gene49010.t1
chr7.3 exon 48495455 48495978 48495455 ID=MS.gene49010.t1.exon3;Parent=MS.gene49010.t1
chr7.3 CDS 48495455 48495978 48495455 ID=cds.MS.gene49010.t1;Parent=MS.gene49010.t1
Gene Sequence

>MS.gene49010

ATGGAGCAAAAGGTTAGTAACAATATTTACTTTAATTTGATATATTTCTGAATCATCAATGAAAAAATATATAATGTATTTTCCATTCCTCTTAACTAAGCCAATTAGTTCTAGTAATAACCATTAAATTAAATGCTTGAATTTTGATTAAGCAACTTTCTTTTCTTGAACTTATGCAGGTTGCTATAATGAGGCTCAAGGTTGACCTTCAATGTAGAAAATGCTGTAAGAAAGTCAAGAAGATCCTTTGCAAATACCCTCGTGAGTCTTTCAATAATAATTATTTTCCTCTCATATTTTATTATCTTTCCTCATACATATTTTGGTATGGTGGTGTTAATATATTATTAGGCTATAGTTACCATTAAATATAAATTTTGTATTGAAAATTGACAAAATTATATGAACAAGTATTTTTTTTCTTTTGCGCAATTTCTCCTAATTTTAGTTTCTTGATTTTTTTTATAAACCTTCTTAATTTGTTTATTTTTTACAATACCTTCTTAATTTGTTAGTATTGCGTTGTTTTTGTTGTTGCAAATTTCATGAATGAAAGAAATTCGAGACCAAATATATGATGAGAAGAATGGTATTGTGACCATAAGAGTGGTGTGCTGCAGCCCAAAGAAGGTAAGAGACAATATTTGTTGTCAAGGGGGTGGTACCATCAAGAGCATAGAAATAGTTGAACCACCAAAGCCTAAGCCAGCACATGAGAAGCCCAAAGAGCAAGAAAAAAAGCCCGTGGCCGAGGCAAAGCCGATTGCACAACCTGGCCCGCCTAAACAAAATCCAAGTCCAGCTCCAGCTCCGGCACCAGCTCCGGCTCAGGCACCGGCTCCACTTCCTTCTGCGACTGCACCAGCTGTAATGTATCCTCACACAACGCCAATGTCCATTCTTGCATATCCTTCGCCAGTTGTTCCTTATGGGCATGTTTATGGTGCTGGACAAGGTGGCCACCCACAGTTTTACGGGAGGCCCGTATATGATAGTTATGGATGGAGTGGGCCTTGTTATGTGGGTCATCACCACGATTGCTTGCGCGAAGAAGAGGCGTCGACATGTACCATAAGTTGA

Protein sequence

>MS.gene49010.t1

MEQKVAIMRLKVDLQCRKCCKKVKKILCKYPQIRDQIYDEKNGIVTIRVVCCSPKKVRDNICCQGGGTIKSIEIVEPPKPKPAHEKPKEQEKKPVAEAKPIAQPGPPKQNPSPAPAPAPAPAQAPAPLPSATAPAVMYPHTTPMSILAYPSPVVPYGHVYGAGQGGHPQFYGRPVYDSYGWSGPCYVGHHHDCLREEEASTCTIS