Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57028.t1 | XP_003615688.2 | 90.9 | 121 | 11 | 0 | 1 | 121 | 16 | 136 | 3.90E-57 | 230.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57028.t1 | Q9LUZ4 | 59.8 | 107 | 42 | 1 | 8 | 113 | 2 | 108 | 2.5e-28 | 126.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57028.t1 | G7JZH7 | 90.9 | 121 | 11 | 0 | 1 | 121 | 16 | 136 | 2.8e-57 | 230.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene04918 | MS.gene57028 | 0.819959 | 8.83E-53 | -1.69E-46 |
| MS.gene04920 | MS.gene57028 | 0.817453 | 3.27E-52 | -1.69E-46 |
| MS.gene049283 | MS.gene57028 | 0.866747 | 2.27E-65 | -1.69E-46 |
| MS.gene049368 | MS.gene57028 | 0.81653 | 5.27E-52 | -1.69E-46 |
| MS.gene049419 | MS.gene57028 | -0.851734 | 7.32E-61 | -1.69E-46 |
| MS.gene049499 | MS.gene57028 | 0.820519 | 6.57E-53 | -1.69E-46 |
| MS.gene049680 | MS.gene57028 | 0.814467 | 1.52E-51 | -1.69E-46 |
| MS.gene049983 | MS.gene57028 | 0.827895 | 1.22E-54 | -1.69E-46 |
| MS.gene04998 | MS.gene57028 | 0.803629 | 3.18E-49 | -1.69E-46 |
| MS.gene050085 | MS.gene57028 | 0.800558 | 1.36E-48 | -1.69E-46 |
| MS.gene050359 | MS.gene57028 | 0.81353 | 2.44E-51 | -1.69E-46 |
| MS.gene050414 | MS.gene57028 | 0.815757 | 7.85E-52 | -1.69E-46 |
| MS.gene050571 | MS.gene57028 | 0.802674 | 5.02E-49 | -1.69E-46 |
| MS.gene050646 | MS.gene57028 | 0.855138 | 7.71E-62 | -1.69E-46 |
| MS.gene050654 | MS.gene57028 | 0.867682 | 1.14E-65 | -1.69E-46 |
| MS.gene050941 | MS.gene57028 | 0.813702 | 2.24E-51 | -1.69E-46 |
| MS.gene050945 | MS.gene57028 | 0.828542 | 8.48E-55 | -1.69E-46 |
| MS.gene051003 | MS.gene57028 | 0.80987 | 1.53E-50 | -1.69E-46 |
| MS.gene051043 | MS.gene57028 | 0.836073 | 1.16E-56 | -1.69E-46 |
| MS.gene051122 | MS.gene57028 | 0.804829 | 1.79E-49 | -1.69E-46 |
| MS.gene051154 | MS.gene57028 | 0.816029 | 6.83E-52 | -1.69E-46 |
| MS.gene051184 | MS.gene57028 | 0.813713 | 2.23E-51 | -1.69E-46 |
| MS.gene051209 | MS.gene57028 | 0.812883 | 3.39E-51 | -1.69E-46 |
| MS.gene051234 | MS.gene57028 | 0.804353 | 2.25E-49 | -1.69E-46 |
| MS.gene051275 | MS.gene57028 | 0.81843 | 1.97E-52 | -1.69E-46 |
| MS.gene051343 | MS.gene57028 | 0.836612 | 8.45E-57 | -1.69E-46 |
| MS.gene051676 | MS.gene57028 | 0.809823 | 1.56E-50 | -1.69E-46 |
| MS.gene051699 | MS.gene57028 | 0.824453 | 8.01E-54 | -1.69E-46 |
| MS.gene051831 | MS.gene57028 | 0.81271 | 3.70E-51 | -1.69E-46 |
| MS.gene051975 | MS.gene57028 | 0.827587 | 1.44E-54 | -1.69E-46 |
| MS.gene052072 | MS.gene57028 | 0.804167 | 2.46E-49 | -1.69E-46 |
| MS.gene052118 | MS.gene57028 | 0.828946 | 6.78E-55 | -1.69E-46 |
| MS.gene052242 | MS.gene57028 | -0.805861 | 1.09E-49 | -1.69E-46 |
| MS.gene052307 | MS.gene57028 | 0.825646 | 4.18E-54 | -1.69E-46 |
| MS.gene052342 | MS.gene57028 | 0.806356 | 8.56E-50 | -1.69E-46 |
| MS.gene052463 | MS.gene57028 | 0.807969 | 3.90E-50 | -1.69E-46 |
| MS.gene052533 | MS.gene57028 | 0.817965 | 2.51E-52 | -1.69E-46 |
| MS.gene052609 | MS.gene57028 | 0.802195 | 6.29E-49 | -1.69E-46 |
| MS.gene052855 | MS.gene57028 | 0.807234 | 5.58E-50 | -1.69E-46 |
| MS.gene052932 | MS.gene57028 | 0.811805 | 5.83E-51 | -1.69E-46 |
| MS.gene053058 | MS.gene57028 | 0.831447 | 1.66E-55 | -1.69E-46 |
| MS.gene053094 | MS.gene57028 | 0.811537 | 6.66E-51 | -1.69E-46 |
| MS.gene053228 | MS.gene57028 | 0.804124 | 2.51E-49 | -1.69E-46 |
| MS.gene053298 | MS.gene57028 | 0.82185 | 3.24E-53 | -1.69E-46 |
| MS.gene053456 | MS.gene57028 | 0.804959 | 1.68E-49 | -1.69E-46 |
| MS.gene053564 | MS.gene57028 | 0.830637 | 2.63E-55 | -1.69E-46 |
| MS.gene053575 | MS.gene57028 | 0.804231 | 2.39E-49 | -1.69E-46 |
| MS.gene053726 | MS.gene57028 | 0.848207 | 7.10E-60 | -1.69E-46 |
| MS.gene053879 | MS.gene57028 | 0.847666 | 1.00E-59 | -1.69E-46 |
| MS.gene053931 | MS.gene57028 | 0.820655 | 6.11E-53 | -1.69E-46 |
| MS.gene053963 | MS.gene57028 | 0.832932 | 7.13E-56 | -1.69E-46 |
| MS.gene054004 | MS.gene57028 | 0.805436 | 1.34E-49 | -1.69E-46 |
| MS.gene054479 | MS.gene57028 | 0.827983 | 1.16E-54 | -1.69E-46 |
| MS.gene05469 | MS.gene57028 | 0.808889 | 2.48E-50 | -1.69E-46 |
| MS.gene054892 | MS.gene57028 | 0.872471 | 3.10E-67 | -1.69E-46 |
| MS.gene054893 | MS.gene57028 | 0.879388 | 1.30E-69 | -1.69E-46 |
| MS.gene054957 | MS.gene57028 | 0.80799 | 3.86E-50 | -1.69E-46 |
| MS.gene055147 | MS.gene57028 | 0.828932 | 6.83E-55 | -1.69E-46 |
| MS.gene055148 | MS.gene57028 | 0.814862 | 1.24E-51 | -1.69E-46 |
| MS.gene055150 | MS.gene57028 | 0.819066 | 1.41E-52 | -1.69E-46 |
| MS.gene055226 | MS.gene57028 | 0.80568 | 1.19E-49 | -1.69E-46 |
| MS.gene055311 | MS.gene57028 | 0.803225 | 3.86E-49 | -1.69E-46 |
| MS.gene055442 | MS.gene57028 | 0.804587 | 2.01E-49 | -1.69E-46 |
| MS.gene055513 | MS.gene57028 | 0.817554 | 3.11E-52 | -1.69E-46 |
| MS.gene055841 | MS.gene57028 | 0.809303 | 2.02E-50 | -1.69E-46 |
| MS.gene055864 | MS.gene57028 | 0.802976 | 4.34E-49 | -1.69E-46 |
| MS.gene056045 | MS.gene57028 | 0.838314 | 3.10E-57 | -1.69E-46 |
| MS.gene056071 | MS.gene57028 | 0.802171 | 6.37E-49 | -1.69E-46 |
| MS.gene056097 | MS.gene57028 | 0.802985 | 4.33E-49 | -1.69E-46 |
| MS.gene056699 | MS.gene57028 | 0.828645 | 8.01E-55 | -1.69E-46 |
| MS.gene056786 | MS.gene57028 | 0.800574 | 1.35E-48 | -1.69E-46 |
| MS.gene05686 | MS.gene57028 | 0.80105 | 1.08E-48 | -1.69E-46 |
| MS.gene056973 | MS.gene57028 | 0.823561 | 1.30E-53 | -1.69E-46 |
| MS.gene056986 | MS.gene57028 | 0.812194 | 4.79E-51 | -1.69E-46 |
| MS.gene057102 | MS.gene57028 | 0.831798 | 1.36E-55 | -1.69E-46 |
| MS.gene057155 | MS.gene57028 | 0.807373 | 5.22E-50 | -1.69E-46 |
| MS.gene057174 | MS.gene57028 | 0.819247 | 1.28E-52 | -1.69E-46 |
| MS.gene057208 | MS.gene57028 | 0.835304 | 1.81E-56 | -1.69E-46 |
| MS.gene057371 | MS.gene57028 | 0.807183 | 5.73E-50 | -1.69E-46 |
| MS.gene057622 | MS.gene57028 | 0.833739 | 4.49E-56 | -1.69E-46 |
| MS.gene057919 | MS.gene57028 | 0.800582 | 1.35E-48 | -1.69E-46 |
| MS.gene057999 | MS.gene57028 | 0.809901 | 1.50E-50 | -1.69E-46 |
| MS.gene058081 | MS.gene57028 | 0.809002 | 2.35E-50 | -1.69E-46 |
| MS.gene058095 | MS.gene57028 | 0.822152 | 2.76E-53 | -1.69E-46 |
| MS.gene058159 | MS.gene57028 | 0.838828 | 2.28E-57 | -1.69E-46 |
| MS.gene058279 | MS.gene57028 | 0.807819 | 4.19E-50 | -1.69E-46 |
| MS.gene058292 | MS.gene57028 | 0.803726 | 3.04E-49 | -1.69E-46 |
| MS.gene058317 | MS.gene57028 | 0.808946 | 2.41E-50 | -1.69E-46 |
| MS.gene058429 | MS.gene57028 | 0.830801 | 2.39E-55 | -1.69E-46 |
| MS.gene058592 | MS.gene57028 | 0.826765 | 2.27E-54 | -1.69E-46 |
| MS.gene058781 | MS.gene57028 | 0.827072 | 1.91E-54 | -1.69E-46 |
| MS.gene05878 | MS.gene57028 | 0.818586 | 1.81E-52 | -1.69E-46 |
| MS.gene059007 | MS.gene57028 | 0.806435 | 8.24E-50 | -1.69E-46 |
| MS.gene059144 | MS.gene57028 | 0.800208 | 1.61E-48 | -1.69E-46 |
| MS.gene059146 | MS.gene57028 | 0.831387 | 1.72E-55 | -1.69E-46 |
| MS.gene059170 | MS.gene57028 | 0.807271 | 5.48E-50 | -1.69E-46 |
| MS.gene05952 | MS.gene57028 | 0.858331 | 8.87E-63 | -1.69E-46 |
| MS.gene05985 | MS.gene57028 | -0.85658 | 2.92E-62 | -1.69E-46 |
| MS.gene059901 | MS.gene57028 | -0.805555 | 1.26E-49 | -1.69E-46 |
| MS.gene059965 | MS.gene57028 | 0.818287 | 2.12E-52 | -1.69E-46 |
| MS.gene060244 | MS.gene57028 | 0.808688 | 2.74E-50 | -1.69E-46 |
| MS.gene060259 | MS.gene57028 | 0.845856 | 3.13E-59 | -1.69E-46 |
| MS.gene060412 | MS.gene57028 | 0.820432 | 6.88E-53 | -1.69E-46 |
| MS.gene060527 | MS.gene57028 | 0.840142 | 1.04E-57 | -1.69E-46 |
| MS.gene060777 | MS.gene57028 | 0.837404 | 5.30E-57 | -1.69E-46 |
| MS.gene060850 | MS.gene57028 | 0.806487 | 8.03E-50 | -1.69E-46 |
| MS.gene060851 | MS.gene57028 | 0.806155 | 9.44E-50 | -1.69E-46 |
| MS.gene060852 | MS.gene57028 | 0.805105 | 1.57E-49 | -1.69E-46 |
| MS.gene060932 | MS.gene57028 | 0.842863 | 1.99E-58 | -1.69E-46 |
| MS.gene060933 | MS.gene57028 | 0.857323 | 1.76E-62 | -1.69E-46 |
| MS.gene060935 | MS.gene57028 | 0.857693 | 1.37E-62 | -1.69E-46 |
| MS.gene060937 | MS.gene57028 | 0.87146 | 6.72E-67 | -1.69E-46 |
| MS.gene061100 | MS.gene57028 | 0.83085 | 2.33E-55 | -1.69E-46 |
| MS.gene061188 | MS.gene57028 | 0.815687 | 8.14E-52 | -1.69E-46 |
| MS.gene06121 | MS.gene57028 | 0.806486 | 8.04E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57028.t1 | MTR_5g071070 | 90.909 | 121 | 11 | 0 | 1 | 121 | 16 | 136 | 1.24e-76 | 240 |
| MS.gene57028.t1 | MTR_1g075890 | 63.393 | 112 | 41 | 0 | 10 | 121 | 23 | 134 | 8.64e-42 | 146 |
| MS.gene57028.t1 | MTR_3g464260 | 64.444 | 90 | 32 | 0 | 24 | 113 | 29 | 118 | 1.28e-30 | 114 |
| MS.gene57028.t1 | MTR_3g464280 | 53.774 | 106 | 34 | 1 | 14 | 119 | 26 | 116 | 2.08e-30 | 114 |
| MS.gene57028.t1 | MTR_3g070880 | 49.038 | 104 | 53 | 0 | 18 | 121 | 8 | 111 | 3.09e-29 | 110 |
| MS.gene57028.t1 | MTR_5g082830 | 54.167 | 96 | 44 | 0 | 15 | 110 | 25 | 120 | 4.89e-29 | 110 |
| MS.gene57028.t1 | MTR_7g092070 | 54.945 | 91 | 41 | 0 | 20 | 110 | 47 | 137 | 2.59e-26 | 102 |
| MS.gene57028.t1 | MTR_5g071070 | 88.889 | 54 | 6 | 0 | 68 | 121 | 1 | 54 | 5.06e-26 | 102 |
| MS.gene57028.t1 | MTR_3g029590 | 47.312 | 93 | 49 | 0 | 18 | 110 | 43 | 135 | 2.08e-21 | 89.0 |
| MS.gene57028.t1 | MTR_0728s0020 | 46.237 | 93 | 49 | 1 | 20 | 111 | 31 | 123 | 1.77e-18 | 80.5 |
| MS.gene57028.t1 | MTR_1g050438 | 46.237 | 93 | 49 | 1 | 20 | 111 | 15 | 107 | 1.93e-18 | 80.5 |
| MS.gene57028.t1 | MTR_3g435590 | 39.362 | 94 | 57 | 0 | 17 | 110 | 29 | 122 | 3.38e-14 | 68.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57028.t1 | AT5G58620 | 59.813 | 107 | 42 | 1 | 8 | 113 | 2 | 108 | 1.36e-34 | 126 |
| MS.gene57028.t1 | AT3G55980 | 56.250 | 96 | 42 | 0 | 15 | 110 | 92 | 187 | 6.95e-31 | 115 |
| MS.gene57028.t1 | AT3G55980 | 56.250 | 96 | 42 | 0 | 15 | 110 | 25 | 120 | 8.47e-31 | 115 |
| MS.gene57028.t1 | AT2G40140 | 57.447 | 94 | 39 | 1 | 20 | 112 | 33 | 126 | 9.12e-30 | 112 |
| MS.gene57028.t1 | AT2G40140 | 57.447 | 94 | 39 | 1 | 20 | 112 | 33 | 126 | 9.12e-30 | 112 |
| MS.gene57028.t1 | AT2G41900 | 51.648 | 91 | 44 | 0 | 20 | 110 | 48 | 138 | 3.82e-23 | 94.0 |
| MS.gene57028.t1 | AT5G12850 | 45.455 | 99 | 53 | 1 | 15 | 113 | 37 | 134 | 2.12e-22 | 92.0 |
Find 0 sgRNAs with CRISPR-Local
Find 42 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAGCAAAATCTTGATAATCT+CGG | + | 37959:642-661 | MS.gene57028:intergenic | 25.0% |
| AAAAAATGCGATTGAAAACG+AGG | - | 37959:395-414 | MS.gene57028:CDS | 30.0% | |
| AAAAATGCGATTGAAAACGA+GGG | - | 37959:396-415 | MS.gene57028:CDS | 30.0% | |
| ACAAATTAGGTTATGAAGAG+AGG | - | 37959:469-488 | MS.gene57028:CDS | 30.0% | |
| ATGTGAATGGGAATAAAGAA+GGG | - | 37959:313-332 | MS.gene57028:CDS | 30.0% | |
| TATGTGAATGGGAATAAAGA+AGG | - | 37959:312-331 | MS.gene57028:CDS | 30.0% | |
| ! | AGCAAAATCTTGATAATCTC+GGG | + | 37959:641-660 | MS.gene57028:intergenic | 30.0% |
| !! | AAAGTTGGTTCGAACAAATT+AGG | - | 37959:456-475 | MS.gene57028:CDS | 30.0% |
| CTTATATTCTTGGAACAGGT+CGG | - | 37959:538-557 | MS.gene57028:CDS | 35.0% | |
| TTATATTCTTGGAACAGGTC+GGG | - | 37959:539-558 | MS.gene57028:CDS | 35.0% | |
| ! | CAAGATTTTGCTTGATGCAT+CGG | - | 37959:649-668 | MS.gene57028:CDS | 35.0% |
| ! | GCAAAATCTTGATAATCTCG+GGG | + | 37959:640-659 | MS.gene57028:intergenic | 35.0% |
| !! | GATGTATCGGCTTATATTCT+TGG | - | 37959:528-547 | MS.gene57028:CDS | 35.0% |
| GTATGGAAGCTTGGATGTAT+CGG | - | 37959:515-534 | MS.gene57028:CDS | 40.0% | |
| GTTGATGTTAATCGGTCTAG+TGG | - | 37959:561-580 | MS.gene57028:CDS | 40.0% | |
| TAAAGAAGGGCAGTATCATG+AGG | - | 37959:326-345 | MS.gene57028:CDS | 40.0% | |
| TCGGCTTATATTCTTGGAAC+AGG | - | 37959:534-553 | MS.gene57028:CDS | 40.0% | |
| TTGTGGTATGGAAGGAAAGT+TGG | - | 37959:441-460 | MS.gene57028:CDS | 40.0% | |
| ! | ATTTTCAGCTGCAGATGATG+TGG | - | 37959:365-384 | MS.gene57028:CDS | 40.0% |
| ATGAAGAGCAGTAGCACCAT+CGG | + | 37959:589-608 | MS.gene57028:intergenic | 45.0% | |
| ATGGGGTGTGAGTATGTGAA+TGG | - | 37959:300-319 | MS.gene57028:CDS | 45.0% | |
| CTCATGGTTGCAGCTATGTA+TGG | - | 37959:498-517 | MS.gene57028:CDS | 45.0% | |
| CTCTTCATTGTGCGGTTGTT+GGG | - | 37959:601-620 | MS.gene57028:CDS | 45.0% | |
| TACATAGCTGCAACCATGAG+AGG | + | 37959:498-517 | MS.gene57028:intergenic | 45.0% | |
| TCTTCATTGTGCGGTTGTTG+GGG | - | 37959:602-621 | MS.gene57028:CDS | 45.0% | |
| TGCAGCTATGTATGGAAGCT+TGG | - | 37959:506-525 | MS.gene57028:CDS | 45.0% | |
| TGCTACTGCTCTTCATTGTG+CGG | - | 37959:593-612 | MS.gene57028:CDS | 45.0% | |
| TGGGGTGTGAGTATGTGAAT+GGG | - | 37959:301-320 | MS.gene57028:CDS | 45.0% | |
| ! | GATTTTGCTTGATGCATCGG+CGG | - | 37959:652-671 | MS.gene57028:CDS | 45.0% |
| !! | ATGTTGATGGGGTTGGTTTG+TGG | - | 37959:424-443 | MS.gene57028:CDS | 45.0% |
| !! | CAGGTCGGGTTGATGTTAAT+CGG | - | 37959:553-572 | MS.gene57028:CDS | 45.0% |
| AACGAGGGTTGCGATGTTGA+TGG | - | 37959:411-430 | MS.gene57028:CDS | 50.0% | |
| ACGAGGGTTGCGATGTTGAT+GGG | - | 37959:412-431 | MS.gene57028:CDS | 50.0% | |
| CTTCATTGTGCGGTTGTTGG+GGG | - | 37959:603-622 | MS.gene57028:CDS | 50.0% | |
| GCTCTTCATTGTGCGGTTGT+TGG | - | 37959:600-619 | MS.gene57028:CDS | 50.0% | |
| TCAGCTGCAGATGATGTGGT+TGG | - | 37959:369-388 | MS.gene57028:CDS | 50.0% | |
| TGAAGAGAGGACACCTCTCA+TGG | - | 37959:482-501 | MS.gene57028:CDS | 50.0% | |
| ! | AATCGGTCTAGTGGATCCGA+TGG | - | 37959:570-589 | MS.gene57028:CDS | 50.0% |
| !! | GATGGGGTTGGTTTGTGGTA+TGG | - | 37959:429-448 | MS.gene57028:CDS | 50.0% |
| !! | GGGTTGGTTTGTGGTATGGA+AGG | - | 37959:433-452 | MS.gene57028:CDS | 50.0% |
| CGAGGGTTGCGATGTTGATG+GGG | - | 37959:413-432 | MS.gene57028:CDS | 55.0% | |
| ! | GGTTGCGATGTTGATGGGGT+TGG | - | 37959:417-436 | MS.gene57028:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 37959 | gene | 300 | 698 | 300 | ID=MS.gene57028 |
| 37959 | mRNA | 300 | 698 | 300 | ID=MS.gene57028.t1;Parent=MS.gene57028 |
| 37959 | exon | 300 | 698 | 300 | ID=MS.gene57028.t1.exon1;Parent=MS.gene57028.t1 |
| 37959 | CDS | 300 | 698 | 300 | ID=cds.MS.gene57028.t1;Parent=MS.gene57028.t1 |
| Gene Sequence |
| Protein sequence |
