AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene63489


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene63489.t1 MTR_3g068150 96.791 187 6 0 1 187 1 187 2.19e-126 355
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene63489.t1 AT2G44920 86.331 139 19 0 50 188 50 188 2.89e-81 240
MS.gene63489.t1 AT2G44920 86.232 138 19 0 50 187 50 187 2.47e-80 238

Find 44 sgRNAs with CRISPR-Local

Find 102 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACTCCTATCTCTCTGTTTC+TGG 0.179667 3.1:-56437375 MS.gene63489:intron
ACTAGGGGACAAGATCTTTC+TGG 0.219620 3.1:-56436833 MS.gene63489:CDS
AAATTTGAGCAATGCTAATT+TGG 0.246193 3.1:-56435820 MS.gene63489:CDS
CATACCGGCGCCTGTAATAT+TGG 0.263857 3.1:+56435758 None:intergenic
TTTAAAGGTGCAAAATTGTT+AGG 0.294153 3.1:-56436613 MS.gene63489:CDS
AAAATTGGCTTGACGCAGTA+TGG 0.314554 3.1:+56436633 None:intergenic
TTCTTCGATGCTGATTTAAC+AGG 0.320279 3.1:-56436583 MS.gene63489:intron
TTTAAGGGATCCAATATTAC+AGG 0.328394 3.1:-56435768 MS.gene63489:CDS
TGATCCTGCACTTGCATTCA+AGG 0.342254 3.1:-56437155 MS.gene63489:intron
GATGCTTGGTGAGTTTGCTT+TGG 0.375494 3.1:+56437259 None:intergenic
TAAGAGATGGTGATGAGGTT+TGG 0.381918 3.1:+56437430 None:intergenic
CATATGGTCAAGGAGTTACT+AGG 0.385923 3.1:-56436850 MS.gene63489:CDS
CTAGTAACTCCTTGACCATA+TGG 0.403791 3.1:+56436851 None:intergenic
CATGTCAGGTGCTGATCTTT+CGG 0.407019 3.1:-56436468 MS.gene63489:intron
TTGGAAGGTGCACTTGTAAC+GGG 0.417101 3.1:-56435801 MS.gene63489:CDS
CTAGGGGACAAGATCTTTCT+GGG 0.423220 3.1:-56436832 MS.gene63489:CDS
CGAACCAGAAACAGAGAGAT+AGG 0.437434 3.1:+56437371 None:intergenic
AACCTCATCACCATCTCTTA+AGG 0.447203 3.1:-56437427 MS.gene63489:CDS
AGAGATAGGAGTTGGTGAGC+AGG 0.464069 3.1:+56437385 None:intergenic
ATGAAGGGTGGAGGGCCATA+TGG 0.474954 3.1:-56436866 MS.gene63489:intron
TTACCTTTGTCACATTTGCC+AGG 0.489767 3.1:+56436417 None:intergenic
AGTTGGTGAGCAGGTTGAAG+AGG 0.491050 3.1:+56437394 None:intergenic
GGTGAGCAGGTTGAAGAGGA+AGG 0.497485 3.1:+56437398 None:intergenic
CAGAAACTCACATTATCATT+CGG 0.504813 3.1:-56437235 MS.gene63489:CDS
TTTGGAAGGTGCACTTGTAA+CGG 0.505123 3.1:-56435802 MS.gene63489:CDS
TTGAGCAATGCTAATTTGGA+AGG 0.506866 3.1:-56435816 MS.gene63489:CDS
TGGTGATGAATGAAGGGTGG+AGG 0.551341 3.1:-56436875 MS.gene63489:intron
AGAAACAGAGAGATAGGAGT+TGG 0.558423 3.1:+56437377 None:intergenic
TGATGAGGTTTGGAAGTTGA+AGG 0.562107 3.1:+56437440 None:intergenic
ATATGGTCAAGGAGTTACTA+GGG 0.565044 3.1:-56436849 MS.gene63489:CDS
TATACCTTGAATGCAAGTGC+AGG 0.570494 3.1:+56437151 None:intergenic
GGATCCAATATTACAGGCGC+CGG 0.582010 3.1:-56435762 MS.gene63489:CDS
GGTGGAGGGCCATATGGTCA+AGG 0.584439 3.1:-56436860 MS.gene63489:CDS
TACCTTTGTCACATTTGCCA+GGG 0.587787 3.1:+56436418 None:intergenic
TTCCCTGGCAAATGTGACAA+AGG 0.601317 3.1:-56436420 MS.gene63489:intron
ATGTGAGTTTCTGAGATGCT+TGG 0.612261 3.1:+56437245 None:intergenic
CTTTCTGGGAAAGATTTCAG+TGG 0.615907 3.1:-56436818 MS.gene63489:CDS
TTTGGAAGTTGAAGGCAAAG+AGG 0.617513 3.1:+56437448 None:intergenic
GGTGATGAATGAAGGGTGGA+GGG 0.622244 3.1:-56436874 MS.gene63489:intron
CATCAAGCAAGACTTCAAAA+CGG 0.635311 3.1:-56436786 MS.gene63489:intron
AAGGTGTTGACCTTAAGAGA+TGG 0.643063 3.1:+56437417 None:intergenic
GACCTTAAGAGATGGTGATG+AGG 0.653487 3.1:+56437425 None:intergenic
ATAGTGATACTACTACATAC+CGG 0.658992 3.1:+56435743 None:intergenic
TATGGTCAAGGAGTTACTAG+GGG 0.721516 3.1:-56436848 MS.gene63489:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GTTAAGAAATTAAATTAAAA+TGG - chr3.1:56437140-56437159 MS.gene63489:intron 10.0%
!! GTTAATAAACTGAATTATAT+TGG + chr3.1:56436923-56436942 None:intergenic 15.0%
!! TATAAGTATAACTGTTTATT+AGG + chr3.1:56437067-56437086 None:intergenic 15.0%
!! TTCTTCAAATAATATAACAA+AGG + chr3.1:56437094-56437113 None:intergenic 15.0%
!!! TTAATTTCTTAACATTCTTT+TGG + chr3.1:56437133-56437152 None:intergenic 15.0%
!!! TTTTTTGGTTAACAAATAAT+GGG - chr3.1:56436317-56436336 MS.gene63489:intron 15.0%
!!! CTTTTTTGGTTAACAAATAA+TGG - chr3.1:56436316-56436335 MS.gene63489:intron 20.0%
!!! GTTAAAGTTTTGTTCTTTTT+TGG - chr3.1:56436302-56436321 MS.gene63489:intron 20.0%
!!! TTTATTAGGTTGTTGAATAA+TGG + chr3.1:56437053-56437072 None:intergenic 20.0%
! AAACATTCATATTGAAGTGT+TGG + chr3.1:56436117-56436136 None:intergenic 25.0%
! AAATTTGAGCAATGCTAATT+TGG - chr3.1:56437402-56437421 MS.gene63489:CDS 25.0%
! AACTGAATTATATTGGACTA+AGG + chr3.1:56436916-56436935 None:intergenic 25.0%
! ACTGAATTATATTGGACTAA+GGG + chr3.1:56436915-56436934 None:intergenic 25.0%
! ATAACAACAAAGCAAAGAAA+AGG + chr3.1:56436953-56436972 None:intergenic 25.0%
! CAACACAATAAATTGAGAAA+TGG + chr3.1:56437361-56437380 None:intergenic 25.0%
! CATTTCTCAATTTATTGTGT+TGG - chr3.1:56437359-56437378 MS.gene63489:intron 25.0%
! CTTCAATATGAATGTTTATC+TGG - chr3.1:56436120-56436139 MS.gene63489:intron 25.0%
! TCAGCTAAATTAATACTTGT+AGG + chr3.1:56437289-56437308 None:intergenic 25.0%
! TTGTTAATTACAGTGATTTG+AGG + chr3.1:56437325-56437344 None:intergenic 25.0%
!! CATTCATTGTGATACATTTT+TGG + chr3.1:56436676-56436695 None:intergenic 25.0%
!! TTTAAAGGTGCAAAATTGTT+AGG - chr3.1:56436609-56436628 MS.gene63489:CDS 25.0%
!! TTTTTCATGCTCATTATGAT+AGG - chr3.1:56436832-56436851 MS.gene63489:CDS 25.0%
!!! ATTAAGCAAAGTTTTGAACA+AGG + chr3.1:56436979-56436998 None:intergenic 25.0%
!!! CAATTTTGCACCTTTAAAAT+TGG + chr3.1:56436607-56436626 None:intergenic 25.0%
AATAGCAGTGATTGAAAGTA+GGG - chr3.1:56436996-56437015 MS.gene63489:intron 30.0%
ATGAATGTAGTATACACACA+TGG - chr3.1:56436689-56436708 MS.gene63489:intron 30.0%
ATGGCAAATTAGAAGATTCA+AGG - chr3.1:56437159-56437178 MS.gene63489:CDS 30.0%
CAGAAACTCACATTATCATT+CGG - chr3.1:56435987-56436006 MS.gene63489:intron 30.0%
TAATAGCAGTGATTGAAAGT+AGG - chr3.1:56436995-56437014 MS.gene63489:intron 30.0%
TACAAACACTAACACAAACA+CGG + chr3.1:56436225-56436244 None:intergenic 30.0%
TCATTATGATAGGATCTGAT+GGG - chr3.1:56436842-56436861 MS.gene63489:CDS 30.0%
TGGCAAATTAGAAGATTCAA+GGG - chr3.1:56437160-56437179 MS.gene63489:CDS 30.0%
TTTAAGGGATCCAATATTAC+AGG - chr3.1:56437454-56437473 MS.gene63489:CDS 30.0%
! AACGGGAAATACATCTTTTA+AGG - chr3.1:56437438-56437457 MS.gene63489:CDS 30.0%
! ACGGGAAATACATCTTTTAA+GGG - chr3.1:56437439-56437458 MS.gene63489:CDS 30.0%
!! AACAAATAATGGGTATGGTT+TGG - chr3.1:56436327-56436346 MS.gene63489:intron 30.0%
!! TGGTTAACAAATAATGGGTA+TGG - chr3.1:56436322-56436341 MS.gene63489:intron 30.0%
!! TTTAAGTTGATTTGATGACG+CGG - chr3.1:56436495-56436514 MS.gene63489:intron 30.0%
!!! CACTACAAGATTTTTTTGAC+AGG + chr3.1:56437227-56437246 None:intergenic 30.0%
!!! TTGTGATACATTTTTGGTGT+TGG + chr3.1:56436670-56436689 None:intergenic 30.0%
ATATGGTCAAGGAGTTACTA+GGG - chr3.1:56436373-56436392 MS.gene63489:intron 35.0%
CATCAAGCAAGACTTCAAAA+CGG - chr3.1:56436436-56436455 MS.gene63489:CDS 35.0%
CTCATTATGATAGGATCTGA+TGG - chr3.1:56436841-56436860 MS.gene63489:CDS 35.0%
GACAAAACATTGACACTTGT+CGG + chr3.1:56436149-56436168 None:intergenic 35.0%
GGGGAGATATTGAATGTAAA+TGG + chr3.1:56436725-56436744 None:intergenic 35.0%
TTGAGCAATGCTAATTTGGA+AGG - chr3.1:56437406-56437425 MS.gene63489:CDS 35.0%
!! ATGGTTTGGTGATGAATGAA+GGG - chr3.1:56436341-56436360 MS.gene63489:intron 35.0%
!! TATGGTTTGGTGATGAATGA+AGG - chr3.1:56436340-56436359 MS.gene63489:intron 35.0%
!! TTCTTCGATGCTGATTTAAC+AGG - chr3.1:56436639-56436658 MS.gene63489:intron 35.0%
AACCTCATCACCATCTCTTA+AGG - chr3.1:56435795-56435814 MS.gene63489:CDS 40.0%
AACTCCTATCTCTCTGTTTC+TGG - chr3.1:56435847-56435866 MS.gene63489:intron 40.0%
AGAAACAGAGAGATAGGAGT+TGG + chr3.1:56435848-56435867 None:intergenic 40.0%
ATGACGCGGATAGTCAAATT+CGG - chr3.1:56436509-56436528 MS.gene63489:intron 40.0%
CACGGACACCAAACAAAATA+CGG + chr3.1:56436207-56436226 None:intergenic 40.0%
CATATGGTCAAGGAGTTACT+AGG - chr3.1:56436372-56436391 MS.gene63489:intron 40.0%
CTAGTAACTCCTTGACCATA+TGG + chr3.1:56436374-56436393 None:intergenic 40.0%
TACCTTTGTCACATTTGCCA+GGG + chr3.1:56436807-56436826 None:intergenic 40.0%
TATACCTTGAATGCAAGTGC+AGG + chr3.1:56436074-56436093 None:intergenic 40.0%
TATGGTCAAGGAGTTACTAG+GGG - chr3.1:56436374-56436393 MS.gene63489:intron 40.0%
TGATGAGGTTTGGAAGTTGA+AGG + chr3.1:56435785-56435804 None:intergenic 40.0%
TTACCTTTGTCACATTTGCC+AGG + chr3.1:56436808-56436827 None:intergenic 40.0%
TTTGGAAGTTGAAGGCAAAG+AGG + chr3.1:56435777-56435796 None:intergenic 40.0%
! CTGCGTCAAGCCAATTTTAA+AGG - chr3.1:56436594-56436613 MS.gene63489:CDS 40.0%
! CTTTCTGGGAAAGATTTCAG+TGG - chr3.1:56436404-56436423 MS.gene63489:intron 40.0%
! TAAGAGATGGTGATGAGGTT+TGG + chr3.1:56435795-56435814 None:intergenic 40.0%
! TGGTGTGTCCGTATTTTGTT+TGG - chr3.1:56436196-56436215 MS.gene63489:intron 40.0%
! TTTGGAAGGTGCACTTGTAA+CGG - chr3.1:56437420-56437439 MS.gene63489:CDS 40.0%
!! AAAATTGGCTTGACGCAGTA+TGG + chr3.1:56436592-56436611 None:intergenic 40.0%
!! AAGGTGTTGACCTTAAGAGA+TGG + chr3.1:56435808-56435827 None:intergenic 40.0%
!! ATGTGAGTTTCTGAGATGCT+TGG + chr3.1:56435980-56435999 None:intergenic 40.0%
!! CGATGCTGATTTAACAGGTT+TGG - chr3.1:56436644-56436663 MS.gene63489:intron 40.0%
ACTAGGGGACAAGATCTTTC+TGG - chr3.1:56436389-56436408 MS.gene63489:intron 45.0%
ATCAGCACCTGACATGACAA+CGG + chr3.1:56436750-56436769 None:intergenic 45.0%
CGAACCAGAAACAGAGAGAT+AGG + chr3.1:56435854-56435873 None:intergenic 45.0%
CTAGGGGACAAGATCTTTCT+GGG - chr3.1:56436390-56436409 MS.gene63489:intron 45.0%
GTATATGTGCTAGTGCTTCG+TGG - chr3.1:56436176-56436195 MS.gene63489:intron 45.0%
TCACTCTACCACTCTTCACA+GGG - chr3.1:56435933-56435952 MS.gene63489:intron 45.0%
TGATCCTGCACTTGCATTCA+AGG - chr3.1:56436067-56436086 MS.gene63489:intron 45.0%
TTCCCTGGCAAATGTGACAA+AGG - chr3.1:56436802-56436821 MS.gene63489:CDS 45.0%
TTGGCATTACTGCTTTCTGC+AGG - chr3.1:56437378-56437397 MS.gene63489:CDS 45.0%
! GACCTTAAGAGATGGTGATG+AGG + chr3.1:56435800-56435819 None:intergenic 45.0%
! GATGCTTGGTGAGTTTGCTT+TGG + chr3.1:56435966-56435985 None:intergenic 45.0%
! TTGGAAGGTGCACTTGTAAC+GGG - chr3.1:56437421-56437440 MS.gene63489:CDS 45.0%
!! CATGTCAGGTGCTGATCTTT+CGG - chr3.1:56436754-56436773 MS.gene63489:intron 45.0%
!! GTTTGGTGATGAATGAAGGG+TGG - chr3.1:56436344-56436363 MS.gene63489:intron 45.0%
!! TAGAAGTGCAGACTTTTCCC+TGG - chr3.1:56436787-56436806 MS.gene63489:CDS 45.0%
CATACCGGCGCCTGTAATAT+TGG + chr3.1:56437467-56437486 None:intergenic 50.0%
CTCACTCTACCACTCTTCAC+AGG - chr3.1:56435932-56435951 MS.gene63489:intron 50.0%
GATTGCTTCCCTGTGAAGAG+TGG + chr3.1:56435944-56435963 None:intergenic 50.0%
GGATCCAATATTACAGGCGC+CGG - chr3.1:56437460-56437479 MS.gene63489:CDS 50.0%
GGTGATGAATGAAGGGTGGA+GGG - chr3.1:56436348-56436367 MS.gene63489:intron 50.0%
TCAGCACCTGACATGACAAC+GGG + chr3.1:56436749-56436768 None:intergenic 50.0%
! TGGTGATGAATGAAGGGTGG+AGG - chr3.1:56436347-56436366 MS.gene63489:intron 50.0%
!! AGAGATAGGAGTTGGTGAGC+AGG + chr3.1:56435840-56435859 None:intergenic 50.0%
!! AGTTGGTGAGCAGGTTGAAG+AGG + chr3.1:56435831-56435850 None:intergenic 50.0%
ACCTGACATGACAACGGGTG+GGG + chr3.1:56436744-56436763 None:intergenic 55.0%
ATGAAGGGTGGAGGGCCATA+TGG - chr3.1:56436356-56436375 MS.gene63489:intron 55.0%
CACCTGACATGACAACGGGT+GGG + chr3.1:56436745-56436764 None:intergenic 55.0%
! GGTGAGCAGGTTGAAGAGGA+AGG + chr3.1:56435827-56435846 None:intergenic 55.0%
GCACCTGACATGACAACGGG+TGG + chr3.1:56436746-56436765 None:intergenic 60.0%
GGTGGAGGGCCATATGGTCA+AGG - chr3.1:56436362-56436381 MS.gene63489:intron 60.0%
TCCCCACCCGTTGTCATGTC+AGG - chr3.1:56436740-56436759 MS.gene63489:intron 60.0%


Chromosome Type Strat End Strand Name
chr3.1 gene 56435755 56437489 56435755 ID=MS.gene63489
chr3.1 mRNA 56435755 56437489 56435755 ID=MS.gene63489.t1;Parent=MS.gene63489
chr3.1 exon 56437376 56437489 56437376 ID=MS.gene63489.t1.exon1;Parent=MS.gene63489.t1
chr3.1 CDS 56437376 56437489 56437376 ID=cds.MS.gene63489.t1;Parent=MS.gene63489.t1
chr3.1 exon 56437156 56437290 56437156 ID=MS.gene63489.t1.exon2;Parent=MS.gene63489.t1
chr3.1 CDS 56437156 56437290 56437156 ID=cds.MS.gene63489.t1;Parent=MS.gene63489.t1
chr3.1 exon 56436787 56436882 56436787 ID=MS.gene63489.t1.exon3;Parent=MS.gene63489.t1
chr3.1 CDS 56436787 56436882 56436787 ID=cds.MS.gene63489.t1;Parent=MS.gene63489.t1
chr3.1 exon 56436584 56436656 56436584 ID=MS.gene63489.t1.exon4;Parent=MS.gene63489.t1
chr3.1 CDS 56436584 56436656 56436584 ID=cds.MS.gene63489.t1;Parent=MS.gene63489.t1
chr3.1 exon 56436421 56436482 56436421 ID=MS.gene63489.t1.exon5;Parent=MS.gene63489.t1
chr3.1 CDS 56436421 56436482 56436421 ID=cds.MS.gene63489.t1;Parent=MS.gene63489.t1
chr3.1 exon 56435755 56435844 56435755 ID=MS.gene63489.t1.exon6;Parent=MS.gene63489.t1
chr3.1 CDS 56435755 56435844 56435755 ID=cds.MS.gene63489.t1;Parent=MS.gene63489.t1
Gene Sequence

>MS.gene63489

ATGGCTCTTTTTCTGAACGCCTCTTTGCCTTCAACTTCCAAACCTCATCACCATCTCTTAAGGTCAACACCTTCCTCTTCAACCTGCTCACCAACTCCTATCTCTCTGTTTCTGGTTCGTATACTATACTAACTATACAATTTTATATTCATGAATCATCTTTCAAGTTTAACAATTCTCACTCTACCACTCTTCACAGGGAAGCAATCCAAAGCAAACTCACCAAGCATCTCAGAAACTCACATTATCATTCGGTGAATCAGTGTCTAGTGCAGCTCTAGTTGCTCTTCTATCTGCTTCTCTCTTCTTCGTTGATCCTGCACTTGCATTCAAGGTATATACTTAATAACTATGTATCGCCAACACTTCAATATGAATGTTTATCTGGTGTCCGACAAGTGTCAATGTTTTGTCGATGTTCGTATATGTGCTAGTGCTTCGTGGTGTGTCCGTATTTTGTTTGGTGTCCGTGTTTGTGTTAGTGTTTGTACTTCGTAGATAACTAATAATAATGTAATTTAACAGTTTGTAGTATTTAGATGAGATTGTTAAAGTTTTGTTCTTTTTTGGTTAACAAATAATGGGTATGGTTTGGTGATGAATGAAGGGTGGAGGGCCATATGGTCAAGGAGTTACTAGGGGACAAGATCTTTCTGGGAAAGATTTCAGTGGCAAGACACTCATCAAGCAAGACTTCAAAACGGTGTAATTTTTTATTTCTTCAAGAAAAAAAAAAAAAGTTTAAGTTGATTTGATGACGCGGATAGTCAAATTCGGTGTTTTAGATCAAAATGTAACATGTTATCTTATTCTTATTACTTGTTATATTACAGTCCATACTGCGTCAAGCCAATTTTAAAGGTGCAAAATTGTTAGGTGCTAGCTTCTTCGATGCTGATTTAACAGGTTTGGCCAACACCAAAAATGTATCACAATGAATGTAGTATACACACATGGTTTGTCATTTCCATTTACATTCAATATCTCCCCACCCGTTGTCATGTCAGGTGCTGATCTTTCGGATACTGATCTTAGAAGTGCAGACTTTTCCCTGGCAAATGTGACAAAGGTAAATCTTTTTTCATGCTCATTATGATAGGATCTGATGGGAATTTAATTTTATATAATTGCTGCTTATTACTAGTTAATAGATATCACCCTTAGTCCAATATAATTCAGTTTATTAACATGAGAACCTTTTCTTTGCTTTGTTGTTATCATCCTTGTTCAAAACTTTGCTTAATAGCAGTGATTGAAAGTAGGGAAATTGATAATAAACAGTTAAACACACAATTCCATTATTCAACAACCTAATAAACAGTTATACTTATATGTTCCTTTGTTATATTATTTGAAGAAGAGAAGTGATTAATGTCCAAAAGAATGTTAAGAAATTAAATTAAAATGGCAAATTAGAAGATTCAAGGGATATATCTTATAAGAGAGCTAATGAAATGTCATAAGTTTGTCCTGTCAAAAAAATCTTGTAGTGAACGCATACAAATCTGTTCTATATACTATACTATTTAGTCCTACAAGTATTAATTTAGCTGAAGCTGAAAATGTGCCTCAAATCACTGTAATTAACAAATGTTTTCTTCTGCCATTTCTCAATTTATTGTGTTGGCATTACTGCTTTCTGCAGGTAAATTTGAGCAATGCTAATTTGGAAGGTGCACTTGTAACGGGAAATACATCTTTTAAGGGATCCAATATTACAGGCGCCGGTATGTAG

Protein sequence

>MS.gene63489.t1

MALFLNASLPSTSKPHHHLLRSTPSSSTCSPTPISLFLGSNPKQTHQASQKLTLSFGESVSSAALVALLSASLFFVDPALAFKGGGPYGQGVTRGQDLSGKDFSGKTLIKQDFKTSILRQANFKGAKLLGASFFDADLTGADLSDTDLRSADFSLANVTKVNLSNANLEGALVTGNTSFKGSNITGAGM